<?xml version="1.0" encoding="UTF-8"?>
<uniprot xmlns="http://uniprot.org/uniprot"
 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
 xsi:schemaLocation="http://uniprot.org/uniprot http://www.uniprot.org/docs/uniprot.xsd">
<entry dataset="Swiss-Prot" created="1988-11-01" modified="2022-02-23" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>P09009</accession>
    <accession>Q3T4P5</accession>
    <name>TERM_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>DNA terminal protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein P8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">VIII</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1987" name="Nucleic Acids Res." volume="15" first="8999" last="9009">
            <title>Primary structure of the DNA terminal protein of bacteriophage PRD1.</title>
            <authorList>
                <person name="Hsieh J.-C."/>
                <person name="Jung G."/>
                <person name="Leavitt M.C."/>
                <person name="Ito J."/>
            </authorList>
            <dbReference type="PubMed" id="3684578"/>
            <dbReference type="DOI" id="10.1093/nar/15.21.8999"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1987" name="Gene" volume="57" first="121" last="130">
            <title>The complete nucleotide sequence of the left very early region of Escherichia coli bacteriophage PRD1 coding for the terminal protein and the DNA polymerase.</title>
            <authorList>
                <person name="Savilahti H."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="3322943"/>
            <dbReference type="DOI" id="10.1016/0378-1119(87)90183-1"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1991" name="Nucleic Acids Res." volume="19" first="3805" last="3810">
            <title>Mapping of the DNA linking tyrosine residue of the PRD1 terminal protein.</title>
            <authorList>
                <person name="Shiue S.Y."/>
                <person name="Hsieh J.C."/>
                <person name="Ito J."/>
            </authorList>
            <dbReference type="PubMed" id="1861973"/>
            <dbReference type="DOI" id="10.1093/nar/19.14.3805"/>
        </citation>
        <scope>COVALENT DNA LINKAGE AT TYR-190</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2010" name="Proc. Natl. Acad. Sci. U.S.A." volume="107" first="16548" last="16553">
            <title>Viral terminal protein directs early organization of phage DNA replication at the bacterial nucleoid.</title>
            <authorList>
                <person name="Munoz-Espin D."/>
                <person name="Holguera I."/>
                <person name="Ballesteros-Plaza D."/>
                <person name="Carballido-Lopez R."/>
                <person name="Salas M."/>
            </authorList>
            <dbReference type="PubMed" id="20823229"/>
            <dbReference type="DOI" id="10.1073/pnas.1010530107"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="18482" last="18487">
            <title>Functional eukaryotic nuclear localization signals are widespread in terminal proteins of bacteriophages.</title>
            <authorList>
                <person name="Redrejo-Rodriguez M."/>
                <person name="Munoz-Espin D."/>
                <person name="Holguera I."/>
                <person name="Mencia M."/>
                <person name="Salas M."/>
            </authorList>
            <dbReference type="PubMed" id="23091024"/>
            <dbReference type="DOI" id="10.1073/pnas.1216635109"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>NUCLEAR LOCALIZATION SIGNAL</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">Acts as a primer for viral genomic replication. DNA terminal protein is covalently linked to the 5'-ends of both strands of the genome through a phosphodiester bond between the beta-hydroxyl group of a tyrosine residue and the 5'-phosphate of the terminal deoxyadenylate. This protein is essential for DNA replication and is involved in the priming of DNA elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer with viral polymerase. Binds to ssDNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the tectivirus DNA terminal protein family.</text>
    </comment>
    <dbReference type="EMBL" id="X06321">
        <property type="protein sequence ID" value="CAA29636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="M22161">
        <property type="protein sequence ID" value="AAA32449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S01613">
        <property type="entry name" value="TPBPPR"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040681.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1194">Viral DNA replication</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000165347" description="DNA terminal protein">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal" evidence="4">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(5'-phospho-DNA)-tyrosine" evidence="2">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="1861973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="20823229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="23091024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="259" mass="29514" checksum="C58491546CE3B710" modified="1988-11-01" version="1">MAKKKPVEKNGLVYKEFQKQVSNLKKAGLIPKTLDVRKVKPTKHYKGLVSKYKDVATGGAKLAAIPNPAVIETLEARGESIIKKGGKAYLKARQQINQRGQIVNPFTVRVTKRGEVVRRYRKTTPEGKPVYITQRELPIKFENMEQWLTELKAAGFQLQPGEQIYFTFNGNYSRRTYTSFDEAFNKFMTYDIIIDAVAGKLKVEDEADLVKSVGFQRISGPEAKAYNRNRIVLPEMQFSQAAKKKYKRRQKRGYGSKGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1989-07-01" modified="2022-02-23" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>P10479</accession>
    <accession>Q3T4P4</accession>
    <name>DPOL_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>DNA-directed DNA polymerase</fullName>
            <ecNumber>2.7.7.7</ecNumber>
            <ecNumber evidence="1">3.1.11.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Protein P1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">I</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1987" name="Proc. Natl. Acad. Sci. U.S.A." volume="84" first="8287" last="8291">
            <title>Bacteriophage PRD1 DNA polymerase: evolution of DNA polymerases.</title>
            <authorList>
                <person name="Jung G."/>
                <person name="Leavitt M.C."/>
                <person name="Hsieh J.-C."/>
                <person name="Ito J."/>
            </authorList>
            <dbReference type="PubMed" id="3479792"/>
            <dbReference type="DOI" id="10.1073/pnas.84.23.8287"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1987" name="Gene" volume="57" first="121" last="130">
            <title>The complete nucleotide sequence of the left very early region of Escherichia coli bacteriophage PRD1 coding for the terminal protein and the DNA polymerase.</title>
            <authorList>
                <person name="Savilahti H."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="3322943"/>
            <dbReference type="DOI" id="10.1016/0378-1119(87)90183-1"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1987" name="Nucleic Acids Res." volume="15" first="8999" last="9009">
            <title>Primary structure of the DNA terminal protein of bacteriophage PRD1.</title>
            <authorList>
                <person name="Hsieh J.-C."/>
                <person name="Jung G."/>
                <person name="Leavitt M.C."/>
                <person name="Ito J."/>
            </authorList>
            <dbReference type="PubMed" id="3684578"/>
            <dbReference type="DOI" id="10.1093/nar/15.21.8999"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="1990" name="Biochem. Biophys. Res. Commun." volume="170" first="1294" last="1300">
            <title>Site-specific mutagenesis of PRD1 DNA polymerase: mutations in highly conserved regions of the family B DNA polymerase.</title>
            <authorList>
                <person name="Jung G."/>
                <person name="Leavitt M.C."/>
                <person name="Schultz M."/>
                <person name="Ito J."/>
            </authorList>
            <dbReference type="PubMed" id="2202298"/>
            <dbReference type="DOI" id="10.1016/0006-291X(90)90534-T"/>
        </citation>
        <scope>MUTAGENESIS</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Replicates the viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purpose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text>The N-terminus contains the 3'-5' exonuclease activity and the C-terminus contains the polymerase activity.</text>
    </comment>
    <comment type="miscellaneous">
        <text>This DNA polymerase requires a protein as a primer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7"/>
    <dbReference type="EC" id="3.1.11.-" evidence="1"/>
    <dbReference type="EMBL" id="J03018">
        <property type="protein sequence ID" value="AAA32452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="M22161">
        <property type="protein sequence ID" value="AAA32450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X06321">
        <property type="protein sequence ID" value="CAA29637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B27328">
        <property type="entry name" value="DJBPD1"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040682.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="SMR" id="P10479"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004868">
        <property type="entry name" value="DNA-dir_DNA_pol_B_mt/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03175">
        <property type="entry name" value="DNA_pol_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-1194">Viral DNA replication</keyword>
    <feature type="chain" id="PRO_0000046545" description="DNA-directed DNA polymerase">
        <location>
            <begin position="1"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P04415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="553" mass="63336" checksum="65E61D0599BDD51E" modified="1989-07-01" version="1">MPRRSRKKVEYKIAAFDFETDPFKHDRIPKPFSWGFYNGEIYKDYWGDDCIEQFIYWLDTIEEPHVIYAHNGGKFDFLFLMKYFRGKLKIVNGRILEVEHGIHKFRDSYAILPVPLAASDEKIEIDYGKMERETREQHKAEILEYLKGDCVTLHKMVSLFIAEFGMRLTIGGTAMNELKQFHPYDPVRKGFDEAMRPFYFGGRCQAFEKGIIEDDIKVYDVNSMYPHAMRNFRHPFSDEFYEANEITEETYFIEWEGENNGAVPVRTKTGLDFNQRSGIFHTSIHEWRAGIDTGTIKPNRIIRTINFTETTTFGAFIDHFFSKRDAAKKAGDLFHNIFYKLILNSSYGKFAQNPENYKEWCITEGGIYLEGYDGEGCEVQEHLDYILWGRPAEMFNYFNVAVAASITGAARSVLLRALAQAERPLYCDTDSIICRDLKNVPLDAYQLGAWDLEATGDKIAIAGKKLYALYAGDNCVKIASKGASLVPRDIGFLMPPDMEPKAAKKVAQQKAKNIGGEKILKVANGGVYDFVNDAPSFKLNGNVQFIKRTIKGT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1990-01-01" modified="2022-02-23" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>P13559</accession>
    <accession>Q3T4P3</accession>
    <name>ENLYS_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Endolysin</fullName>
            <ecNumber evidence="1 2">3.2.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Beta-1,4-N-acetylmuramidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Lysozyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Lytic enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Muramidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Protein P15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">XV</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1989" name="Eur. J. Biochem." volume="180" first="149" last="152">
            <title>Comparison of the amino acid sequence of the lytic enzyme from broad-host-range bacteriophage PRD1 with sequences of other cell-wall-peptidoglycan lytic enzymes.</title>
            <authorList>
                <person name="Pakula T.M."/>
                <person name="Savilahti H."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="2651121"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1989.tb14625.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1987" name="Gene" volume="57" first="121" last="130">
            <title>The complete nucleotide sequence of the left very early region of Escherichia coli bacteriophage PRD1 coding for the terminal protein and the DNA polymerase.</title>
            <authorList>
                <person name="Savilahti H."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="3322943"/>
            <dbReference type="DOI" id="10.1016/0378-1119(87)90183-1"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-98</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1994" name="Eur. J. Biochem." volume="225" first="341" last="346">
            <title>Gene XV of bacteriophage PRD1 encodes a lytic enzyme with muramidase activity.</title>
            <authorList>
                <person name="Caldentey J."/>
                <person name="Hanninen A.L."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="7925454"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1994.00341.x"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2002" name="J. Bacteriol." volume="184" first="104" last="110">
            <title>The lytic enzyme of bacteriophage PRD1 is associated with the viral membrane.</title>
            <authorList>
                <person name="Rydman P.S."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="11741849"/>
            <dbReference type="DOI" id="10.1128/JB.184.1.104-110.2002"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Endolysin involved in host peptidoglycan digestion after permeabilization of the cytoplasmic membrane by holin. Involved in host cell lysis and liberation of progeny phages. Displays strong lytic activity against chloroform-treated Gram-negative cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Optimum pH is 7.0-8.0.</text>
        </phDependence>
        <temperatureDependence>
            <text>Inactivation temperature above 4 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">May be associated with the viral membrane via P20 and P22.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 24 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="1 2"/>
    <dbReference type="EMBL" id="X14980">
        <property type="protein sequence ID" value="CAA33104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="M22161">
        <property type="protein sequence ID" value="AAA32451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S03568">
        <property type="entry name" value="LYBPD1"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040683.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="SMR" id="P13559"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024408">
        <property type="entry name" value="Muramidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016284">
        <property type="entry name" value="Phage_PRD1_P15_lysozyme"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11860">
        <property type="entry name" value="Muramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001069">
        <property type="entry name" value="Lytic_enz_p15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0929">Antimicrobial</keyword>
    <keyword id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword id="KW-0204">Cytolysis</keyword>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0578">Host cell lysis by virus</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000165348" description="Endolysin">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="11741849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="7925454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="149" mass="17269" checksum="50ABB718032939B5" modified="1991-02-01" version="2">MQYTLWDIISRVESNGNLKALRFEPEYYQRRMERGDWDNSIIQNIRAANKCSLGTARMIYCSSWGAVQIMGFNLYLNGAFNLSVAHFMENEAYQVNEFRRFLLKNGLTEYTPERLASDKAARVKFAKVYNGAESYADLILQACQFYGVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1990-08-01" modified="2022-02-23" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>P17637</accession>
    <accession>Q3T4L5</accession>
    <name>VP12_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Single-stranded DNA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein P12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">XII</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1989" name="Gene" volume="85" first="53" last="58">
            <title>The organization of the right-end early region of bacteriophage PRD1 genome.</title>
            <authorList>
                <person name="Pakula T.M."/>
                <person name="Savilahti H."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="2695404"/>
            <dbReference type="DOI" id="10.1016/0378-1119(89)90463-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1990" name="J. Bacteriol." volume="172" first="1889" last="1898">
            <title>Nucleotide sequence and transcription of the right early region of bacteriophage PRD1.</title>
            <authorList>
                <person name="Gerendasy D."/>
                <person name="Ito J."/>
            </authorList>
            <dbReference type="PubMed" id="2180910"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text>Binds to single-stranded DNA (ssDNA). Has a regulatory effect on phage DNA metabolism and transcription of early genes.</text>
    </comment>
    <dbReference type="EMBL" id="M33428">
        <property type="protein sequence ID" value="AAA32447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="M30146">
        <property type="protein sequence ID" value="AAA32453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A35148">
        <property type="entry name" value="A35148"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040702.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000165354" description="Single-stranded DNA-binding protein">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <sequence length="160" mass="16650" checksum="461004D25FC9F470" modified="1990-08-01" version="1">MEIVSKLTLKTIGAQPKPHSVKENTALASIYGRVRGKKVGQSTFGDFIKFEGEFEGVNIATGEVFRSGALILPKVLESLLAGAVDGENTVDFAVEIWAKPSEKGNTGYEYGVKPLIEPAASDELAALRNQVKAALPAPAAAGEAAAEAKPAAKAKAKAEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1990-08-01" modified="2022-02-23" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>P17638</accession>
    <accession>Q3T4L6</accession>
    <name>VP19_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Protein P19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">XIX</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1989" name="Gene" volume="85" first="53" last="58">
            <title>The organization of the right-end early region of bacteriophage PRD1 genome.</title>
            <authorList>
                <person name="Pakula T.M."/>
                <person name="Savilahti H."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="2695404"/>
            <dbReference type="DOI" id="10.1016/0378-1119(89)90463-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1990" name="J. Bacteriol." volume="172" first="1889" last="1898">
            <title>Nucleotide sequence and transcription of the right early region of bacteriophage PRD1.</title>
            <authorList>
                <person name="Gerendasy D."/>
                <person name="Ito J."/>
            </authorList>
            <dbReference type="PubMed" id="2180910"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text>Binds to single-stranded DNA (ssDNA).</text>
    </comment>
    <dbReference type="EMBL" id="M33428">
        <property type="protein sequence ID" value="AAA32448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="M30146">
        <property type="protein sequence ID" value="AAA32454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="JQ0187">
        <property type="entry name" value="JQ0187"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040701.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000165356" description="Protein P19">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2." evidence="1" ref="2">
        <original>YQVKGAIR</original>
        <variation>IKSRALSA</variation>
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2." evidence="1" ref="2">
        <location>
            <begin position="53"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="94" mass="10461" checksum="051999B024EA170E" modified="1990-08-01" version="1">MEKQTENTRPECPKAFYFVSIPGDFGQTPFASSLMYGSTALAAVYQVKGAIRVVSEHFDLRFADNGFTPAGVTQAEWLGKLITETFGFRLELFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1991-08-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>P22535</accession>
    <accession>Q3T4N2</accession>
    <name>CAPSD_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Major capsid protein P3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein P3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">III</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1990" name="Virology" volume="177" first="445" last="451">
            <title>Capsomer proteins of bacteriophage PRD1, a bacterial virus with a membrane.</title>
            <authorList>
                <person name="Bamford J.K.H."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="2196741"/>
            <dbReference type="DOI" id="10.1016/0042-6822(90)90508-O"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 2-11</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1993" name="J. Mol. Biol." volume="230" first="349" last="352">
            <title>Crystallization of the major coat protein of PRD1, a bacteriophage with an internal membrane.</title>
            <authorList>
                <person name="Stewart P.L."/>
                <person name="Ghosh S."/>
                <person name="Bamford D.H."/>
                <person name="Burnett R.M."/>
            </authorList>
            <dbReference type="PubMed" id="8450547"/>
            <dbReference type="DOI" id="10.1006/jmbi.1993.1148"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS)</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2002" name="Acta Crystallogr. D" volume="58" first="39" last="59">
            <title>The X-ray crystal structure of P3, the major coat protein of the lipid-containing bacteriophage PRD1, at 1.65 A resolution.</title>
            <authorList>
                <person name="Benson S.D."/>
                <person name="Bamford J.K.H."/>
                <person name="Bamford D.H."/>
                <person name="Burnett R.M."/>
            </authorList>
            <dbReference type="PubMed" id="11752778"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (1.65 ANGSTROMS)</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2004" name="Nature" volume="432" first="68" last="74">
            <title>Insights into assembly from structural analysis of bacteriophage PRD1.</title>
            <authorList>
                <person name="Abrescia N.G.A."/>
                <person name="Cockburn J.J.B."/>
                <person name="Grimes J.M."/>
                <person name="Sutton G.C."/>
                <person name="Diprose J.M."/>
                <person name="Butcher S.J."/>
                <person name="Fuller S.D."/>
                <person name="San Martin C."/>
                <person name="Burnett R.M."/>
                <person name="Stuart D.I."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K.H."/>
            </authorList>
            <dbReference type="PubMed" id="15525981"/>
            <dbReference type="DOI" id="10.1038/nature03056"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (4.2 ANGSTROMS)</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2004" name="Nature" volume="432" first="122" last="125">
            <title>Membrane structure and interactions with protein and DNA in bacteriophage PRD1.</title>
            <authorList>
                <person name="Cockburn J.J."/>
                <person name="Abrescia N.G."/>
                <person name="Grimes J.M."/>
                <person name="Sutton G.C."/>
                <person name="Diprose J.M."/>
                <person name="Benevides J.M."/>
                <person name="Thomas G.J. Jr."/>
                <person name="Bamford J.K.H."/>
                <person name="Bamford D.H."/>
                <person name="Stuart D.I."/>
            </authorList>
            <dbReference type="PubMed" id="15525993"/>
            <dbReference type="DOI" id="10.1038/nature03053"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (4.2 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text>Major capsid protein self-assembles to form an icosahedral capsid with a pseudo T=25 symmetry, about 66 nm in diameter, and consisting of 240 capsid proteins trimers. The capsid encapsulates an inner membrane and the genomic dsDNA genome. The major coat protein P3 and two assembly factors (P10 and P17) are needed during the assembly of the virus particle inside the host cell, when the capsid protein multimers are capable of enclosing the host-derived membrane, containing the virus-encoded membrane-associated proteins.</text>
    </comment>
    <comment type="subunit">
        <text>Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="online information" name="Virus Particle ExploreR db">
        <link uri="https://viperdb.scripps.edu/Info_Page.php?VDB=1hb9"/>
        <text>Icosahedral capsid structure</text>
    </comment>
    <comment type="online information" name="Virus Particle ExploreR db">
        <link uri="https://viperdb.scripps.edu/Info_Page.php?VDB=1gw7"/>
        <text>Icosahedral capsid structure</text>
    </comment>
    <comment type="online information" name="Virus Particle ExploreR db">
        <link uri="https://viperdb.scripps.edu/Info_Page.php?VDB=1gw8"/>
        <text>Icosahedral capsid structure of SUS607 mutant</text>
    </comment>
    <comment type="online information" name="Virus Particle ExploreR db">
        <link uri="https://viperdb.scripps.edu/Info_Page.php?VDB=1hb5"/>
        <text>Icosahedral virus capsid P3-shell structure</text>
    </comment>
    <comment type="online information" name="Virus Particle ExploreR db">
        <link uri="https://viperdb.scripps.edu/Info_Page.php?VDB=1hb7"/>
        <text>Icosahedral capsid structure of SUS1 mutant</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="M55567">
        <property type="protein sequence ID" value="AAA32445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A46345">
        <property type="entry name" value="A46345"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040692.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="PDB" id="1CJD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A/B/C=2-395"/>
    </dbReference>
    <dbReference type="PDB" id="1GW7">
        <property type="method" value="EM"/>
        <property type="resolution" value="13.50 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=2-395"/>
    </dbReference>
    <dbReference type="PDB" id="1GW8">
        <property type="method" value="EM"/>
        <property type="resolution" value="13.30 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=2-395"/>
    </dbReference>
    <dbReference type="PDB" id="1HB5">
        <property type="method" value="EM"/>
        <property type="resolution" value="12.00 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I=2-395"/>
    </dbReference>
    <dbReference type="PDB" id="1HB7">
        <property type="method" value="EM"/>
        <property type="resolution" value="14.00 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=2-395"/>
    </dbReference>
    <dbReference type="PDB" id="1HB9">
        <property type="method" value="EM"/>
        <property type="resolution" value="25.00 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=2-395"/>
    </dbReference>
    <dbReference type="PDB" id="1HQN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B/C=2-395"/>
    </dbReference>
    <dbReference type="PDB" id="1HX6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.65 A"/>
        <property type="chains" value="A/B/C=2-395"/>
    </dbReference>
    <dbReference type="PDB" id="1W8X">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="4.20 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-395"/>
    </dbReference>
    <dbReference type="PDBsum" id="1CJD"/>
    <dbReference type="PDBsum" id="1GW7"/>
    <dbReference type="PDBsum" id="1GW8"/>
    <dbReference type="PDBsum" id="1HB5"/>
    <dbReference type="PDBsum" id="1HB7"/>
    <dbReference type="PDBsum" id="1HB9"/>
    <dbReference type="PDBsum" id="1HQN"/>
    <dbReference type="PDBsum" id="1HX6"/>
    <dbReference type="PDBsum" id="1W8X"/>
    <dbReference type="SMR" id="P22535"/>
    <dbReference type="PRIDE" id="P22535"/>
    <dbReference type="EvolutionaryTrace" id="P22535"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019028">
        <property type="term" value="C:viral capsid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.9.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015108">
        <property type="entry name" value="Phage_PRD1_P3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016115">
        <property type="entry name" value="Phage_PRD1_P3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029053">
        <property type="entry name" value="Viral_coat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016112">
        <property type="entry name" value="VP_dsDNA_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09018">
        <property type="entry name" value="Phage_Capsid_P3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49749">
        <property type="entry name" value="SSF49749"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="initiator methionine" description="Removed; by host" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000165345" description="Major capsid protein P3">
        <location>
            <begin position="2"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="49"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="54"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="85"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="95"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="108"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="147"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="152"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="191"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="199"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="202"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="214"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="220"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="244"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="251"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="274"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="303"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="311"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="314"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="333"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="340"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="346"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="352"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="357"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="2196741"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="1CJD"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1HX6"/>
        </source>
    </evidence>
    <sequence length="395" mass="43448" checksum="44731365E74C4375" modified="2007-01-23" version="2">MAQVQQLTPAQQAALRNQQAMAANLQARQIVLQQSYPVIQQVETQTFDPANRSVFDVTPANVGIVKGFLVKVTAAITNNHATEAVALTDFGPANLVQRVIYYDPDNQRHTETSGWHLHFVNTAKQGAPFLSSMVTDSPIKYGDVMNVIDAPATIAAGATGELTMYYWVPLAYSETDLTGAVLANVPQSKQRLKLEFANNNTAFAAVGANPLEAIYQGAGAADCEFEEISYTVYQSYLDQLPVGQNGYILPLIDLSTLYNLENSAQAGLTPNVDFVVQYANLYRYLSTIAVFDNGGSFNAGTDINYLSQRTANFSDTRKLDPKTWAAQTRRRIATDFPKGVYYCDNRDKPIYTLQYGNVGFVVNPKTVNQNARLLMGYEYFTSRTELVNAGTISTT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1991-08-01" modified="2021-09-29" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>P22536</accession>
    <accession>Q3T4P0</accession>
    <name>P5_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Spike protein P5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein P5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">V</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1990" name="Virology" volume="177" first="445" last="451">
            <title>Capsomer proteins of bacteriophage PRD1, a bacterial virus with a membrane.</title>
            <authorList>
                <person name="Bamford J.K.H."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="2196741"/>
            <dbReference type="DOI" id="10.1016/0042-6822(90)90508-O"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 2-6</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1990" name="Nature" volume="344" first="497" last="497">
            <title>Collagenous proteins multiply.</title>
            <authorList>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K.H."/>
            </authorList>
            <dbReference type="PubMed" id="2320123"/>
            <dbReference type="DOI" id="10.1038/344497b0"/>
        </citation>
        <scope>COLLAGENOUS REGION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="Biochemistry" volume="39" first="10566" last="10573">
            <title>Assembly of bacteriophage PRD1 spike complex: role of the multidomain protein P5.</title>
            <authorList>
                <person name="Caldentey J."/>
                <person name="Tuma R."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="10956048"/>
            <dbReference type="DOI" id="10.1021/bi000711+"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2005" name="Mol. Cell" volume="18" first="161" last="170">
            <title>The structure of the bacteriophage PRD1 spike sheds light on the evolution of viral capsid architecture.</title>
            <authorList>
                <person name="Merckel M.C."/>
                <person name="Huiskonen J.T."/>
                <person name="Bamford D.H."/>
                <person name="Goldman A."/>
                <person name="Tuma R."/>
            </authorList>
            <dbReference type="PubMed" id="15837420"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2005.03.019"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 197-339</scope>
    </reference>
    <comment type="function">
        <text evidence="1">In association with P31 and P2, forms the spike complexes located at the 5-fold vertices of the capsid. Essential for viral infectivity.</text>
    </comment>
    <comment type="subunit">
        <text>Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text>The short collagen-like region is proposed to act as a trimerization signal leading to the fulfillment of the symmetry requirement of the minor capsomer.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="M55568">
        <property type="protein sequence ID" value="AAA32446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B46345">
        <property type="entry name" value="B46345"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040686.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="PDB" id="1YQ5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=197-340"/>
    </dbReference>
    <dbReference type="PDB" id="1YQ6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B/C=197-340"/>
    </dbReference>
    <dbReference type="PDB" id="1YQ8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A=142-340"/>
    </dbReference>
    <dbReference type="PDBsum" id="1YQ5"/>
    <dbReference type="PDBsum" id="1YQ6"/>
    <dbReference type="PDBsum" id="1YQ8"/>
    <dbReference type="SMR" id="P22536"/>
    <dbReference type="EvolutionaryTrace" id="P22536"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015044">
        <property type="entry name" value="Phage_PRD1_P5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015043">
        <property type="entry name" value="Phage_PRD1_P5_spike_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038663">
        <property type="entry name" value="PRD1_P5_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08948">
        <property type="entry name" value="DUF1859"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08949">
        <property type="entry name" value="DUF1860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="initiator methionine" description="Removed; by host" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000165346" description="Spike protein P5">
        <location>
            <begin position="2"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Collagen-like">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Domain-1">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Domain-2">
        <location>
            <begin position="142"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="153"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="158"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="176"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="184"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="200"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="209"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="215"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="244"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="282"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="311"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="327"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="331"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="10956048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="2196741"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1YQ5"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1YQ8"/>
        </source>
    </evidence>
    <sequence length="340" mass="34449" checksum="769A2AFD3FFC6302" modified="2007-01-23" version="2">MANQQIGGSTVTYNGAIPMGGPVAINSVIEIAGTEVLVDLKLDYATGKISGVQTLYIDLRDFLGDVTVTMPDTGQRITARAGTQGYYPVLSTNLMKFIVSATIDGKFPMNFINFPIALGVWPSGIKGDKGDPGAPGPAGGTVVVEDSGASFGESLLDTTSEPGKILVKRISGGSGITVTDYGDQVEIEASGGGGGGGGVTDALSLMYSTSTGGPASIAANALTDFDLSGALTVNSVGTGLTKSAAGIQLAAGKSGLYQITMTVKNNTVTTGNYLLRVKYGSSDFVVACPASSLTAGGTISLLIYCNVLGVPSLDVLKFSLCNDGAALSNYIINITAAKIN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2021-09-29" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>P27378</accession>
    <accession>Q3T4P2</accession>
    <name>P2_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Adsorption protein P2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein P2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">II</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 2-10</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="J. Biol. Chem." volume="276" first="46187" last="46195">
            <title>Solution structure of bacteriophage PRD1 vertex complex.</title>
            <authorList>
                <person name="Sokolova A."/>
                <person name="Malfois M."/>
                <person name="Caldentey J."/>
                <person name="Svergun D.I."/>
                <person name="Koch M.H."/>
                <person name="Bamford D.H."/>
                <person name="Tuma R."/>
            </authorList>
            <dbReference type="PubMed" id="11577098"/>
            <dbReference type="DOI" id="10.1074/jbc.M106848200"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="J. Struct. Biol." volume="131" first="159" last="163">
            <title>Crystallization and preliminary X-ray analysis of receptor-binding protein P2 of bacteriophage PRD1.</title>
            <authorList>
                <person name="Xu L."/>
                <person name="Butcher S.J."/>
                <person name="Benson S.D."/>
                <person name="Bamford D.H."/>
                <person name="Burnett R.M."/>
            </authorList>
            <dbReference type="PubMed" id="11042087"/>
            <dbReference type="DOI" id="10.1006/jsbi.2000.4275"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS)</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2003" name="Structure" volume="11" first="309" last="322">
            <title>The receptor binding protein P2 of PRD1, a virus targeting antibiotic-resistant bacteria, has a novel fold suggesting multiple functions.</title>
            <authorList>
                <person name="Xu L."/>
                <person name="Benson S.D."/>
                <person name="Butcher S.J."/>
                <person name="Bamford D.H."/>
                <person name="Burnett R.M."/>
            </authorList>
            <dbReference type="PubMed" id="12623018"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(03)00023-6"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Adsorption protein. In association with P31 and trimeric P5, forms the spike complexes located at the 5-fold vertices of the capsid. Involved in recognition and attachment to the receptor on the surface of the host. Likely triggers the processes of vertex disassembly, membrane tube formation, and subsequent DNA injection. Essential for viral infectivity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D40477">
        <property type="entry name" value="WMBPQ2"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040684.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="PDB" id="1N7U">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A=2-555"/>
    </dbReference>
    <dbReference type="PDB" id="1N7V">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A=2-556"/>
    </dbReference>
    <dbReference type="PDBsum" id="1N7U"/>
    <dbReference type="PDBsum" id="1N7V"/>
    <dbReference type="SMR" id="P27378"/>
    <dbReference type="PRIDE" id="P27378"/>
    <dbReference type="EvolutionaryTrace" id="P27378"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.105.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036325">
        <property type="entry name" value="P2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015297">
        <property type="entry name" value="Phage_PRD1_P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015948">
        <property type="entry name" value="Phage_PRD1_P2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015949">
        <property type="entry name" value="Phage_PRD1_P2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09214">
        <property type="entry name" value="Prd1-P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89428">
        <property type="entry name" value="SSF89428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1161">Viral attachment to host cell</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="initiator methionine" description="Removed; by host" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000165349" description="Adsorption protein P2">
        <location>
            <begin position="2"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="disulfide bond">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="3"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="8"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="14"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="28"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="33"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="41"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="63"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="86"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="118"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="123"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="159"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="170"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="192"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="197"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="202"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="210"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="219"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="245"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="259"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="263"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="284"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="294"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="324"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="333"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="340"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="348"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="354"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="398"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="404"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="420"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="425"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="434"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="446"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="451"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="467"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="474"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="490"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="501"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="507"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="513"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="522"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="529"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="547"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="11577098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="1853567"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1N7U"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1N7V"/>
        </source>
    </evidence>
    <sequence length="591" mass="63822" checksum="FAF2DAACBE83DD79" modified="2007-01-23" version="3">MANFNVPKLGVFPVAAVFDIDNVPEDSSATGSRWLPSIYQGGNYWGGGPQALHAQVSNFDSSNRLPYNPRTENNPAGNCAFAFNPFGQYISNISSAQSVHRRIYGIDLNDEPLFSPNAASITNGGNPTMSQDTGYHNIGPINTAYKAEIFRPVNPLPMSDTAPDPETLEPGQTEPLIKSDGVYSNSGIASFIFDRPVTEPNPNWPPLPPPVIPIIYPTPALGIGAAAAYGFGYQVTVYRWEEIPVEFIADPETCPAQPTTDKVIIRTTDLNPEGSPCAYEAGIILVRQTSNPMNAVAGRLVPYVEDIAVDIFLTGKFFTLNPPLRITNNYFADDEVKENTVTIGNYTTTLSSAYYAVYKTDGYGGATCFIASGGAGISALVQLQDNSVLDVLYYSLPLSLGGSKAAIDEWVANNCGLFPMSGGLDKTTLLEIPRRQLEAINPQDGPGQYDLFILDDSGAYASFSSFIGYPEAAYYVAGAATFMDVENPDEIIFILRNGAGWYACEIGDALKIADDEFDSVDYFAYRGGVMFIGSARYTEGGDPLPIKYRAIIPGLPRGRLPRVVLEYQAVGMSFIPCQTHCLGKGGIISKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>P27379</accession>
    <accession>Q3T4N7</accession>
    <name>PKG6_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Packaging efficiency factor P6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">VI</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 1-6</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="J. Virol." volume="77" first="6314" last="6321">
            <title>The unique vertex of bacterial virus PRD1 is connected to the viral internal membrane.</title>
            <authorList>
                <person name="Stromsten N.J."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K.H."/>
            </authorList>
            <dbReference type="PubMed" id="12743288"/>
            <dbReference type="DOI" id="10.1128/JVI.77.11.6314-6321.2003"/>
        </citation>
        <scope>IDENTIFICATION IN THE PORTAL COMPLEX</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2014" name="PLoS Biol." volume="12" first="E1002024" last="E1002024">
            <title>A structural model of the genome packaging process in a membrane-containing double stranded DNA virus.</title>
            <authorList>
                <person name="Hong C."/>
                <person name="Oksanen H.M."/>
                <person name="Liu X."/>
                <person name="Jakana J."/>
                <person name="Bamford D.H."/>
                <person name="Chiu W."/>
            </authorList>
            <dbReference type="PubMed" id="25514469"/>
            <dbReference type="DOI" id="10.1371/journal.pbio.1002024"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>IDENTIFICATION IN THE PORTAL COMPLEX</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Together with the packaging ATPase P9, forms the external part of the portal vertex that is embeded in the capsid and which plays critical roles in genome packaging and genome ejection. Both proteins multimerize as a single ring-shaped heterdodecamer arranged around a central channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Heterodimer of P6 and P9; further multimerizes as hexamers of heterodimers. Part of the dodecameric portal complex that is composed of the packaging efficiency factor P6, the DNA packaging ATPase P9, and the internal heterododecamer P20/P22 which spans the virion inner membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Virion</location>
        </subcellularLocation>
        <text evidence="2">Localizes at the unique vertex of the capsid, which extends to the internal membrane via two small integral membrane proteins, P20 and P22. Packaging efficiency factor P6 attaches to the virion via these proteins and is, in turn, necessary for the attachment of DNA packaging protein P9 to the capsid.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H40477">
        <property type="entry name" value="WMBPP6"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040688.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="SMR" id="P27379"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0231">Viral genome packaging</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000165350" description="Packaging efficiency factor P6">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12743288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="25514469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="166" mass="17573" checksum="DC6D2DBE282195DA" modified="1992-08-01" version="1">MDTEEIKEEMQEAAEAAIENAVETAELETAAIKAEGAAAAAEQSAEQAAVMAATLAASVEANAAQQIAEHSEQVQTQEEKISWLENQVMAMASNLQMMQEAVTALTVSQSLTPEPSPVPAVEVEAMPEAVTVEILPESAGDQQEAEPVPSVGDQQETAPRKRFRAI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2022-02-23" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>P27380</accession>
    <accession>Q3T4M0</accession>
    <accession>Q3T4M1</accession>
    <name>EXLYS_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Transglycosylase</fullName>
            <ecNumber evidence="3">4.2.2.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Protein P7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">VII</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PARTIAL PROTEIN SEQUENCE</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1994" name="Trends Biochem. Sci." volume="19" first="106" last="107">
            <title>A conserved domain in putative bacterial and bacteriophage transglycosylases.</title>
            <authorList>
                <person name="Koonin E.V."/>
                <person name="Rudd K.E."/>
            </authorList>
            <dbReference type="PubMed" id="8203016"/>
            <dbReference type="DOI" id="10.1016/0968-0004(94)90201-1"/>
        </citation>
        <scope>SIMILARITY TO SLT</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="Mol. Microbiol." volume="37" first="356" last="363">
            <title>Bacteriophage PRD1 DNA entry uses a viral membrane-associated transglycosylase activity.</title>
            <authorList>
                <person name="Rydman P.S."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="10931330"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.2000.01996.x"/>
        </citation>
        <scope>ALTERNATIVE INITIATION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2002" name="J. Bacteriol." volume="184" first="104" last="110">
            <title>The lytic enzyme of bacteriophage PRD1 is associated with the viral membrane.</title>
            <authorList>
                <person name="Rydman P.S."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="11741849"/>
            <dbReference type="DOI" id="10.1128/JB.184.1.104-110.2002"/>
        </citation>
        <scope>SUBCELLULAR LOCATION (P7)</scope>
        <scope>SUBCELLULAR LOCATION (P14)</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2002" name="Mol. Microbiol." volume="46" first="1199" last="1209">
            <title>Sequential model of phage PRD1 DNA delivery: active involvement of the viral membrane.</title>
            <authorList>
                <person name="Grahn A.M."/>
                <person name="Daugelavicius R."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="12453208"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.2002.03250.x"/>
        </citation>
        <scope>FUNCTION (ISOFORM P7)</scope>
    </reference>
    <comment type="function">
        <molecule>Isoform Transglycosylase P7</molecule>
        <text evidence="5">Component of the phage ejection machinery which acts as an exolysin. Muralytic protein involved in host peptidoglycan digestion necessary for viral DNA entry into the host cell.</text>
    </comment>
    <comment type="function">
        <molecule>Isoform Protein P14</molecule>
        <text evidence="5">Does not display any enzymatic activity. Required for DNA injection in the membrane transformation event. Involved in the formation of the membrane tail tube to connect the virus interior with the host cytosol. Essential for viral infectivity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Exolytic cleavage of the (1-&gt;4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.</text>
            <dbReference type="EC" id="4.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heteromultimer composed of proteins P7 and P14.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Isoform Transglycosylase P7</molecule>
        <subcellularLocation>
            <location evidence="4">Virion membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="6">Part of the capsid inner membrane. Approximately 20 copies per virion.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Isoform Protein P14</molecule>
        <subcellularLocation>
            <location evidence="4">Virion membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="6">Approximately 20 copies per virion.</text>
    </comment>
    <comment type="alternative products">
        <event type="alternative initiation"/>
        <isoform>
            <id>P27380-1</id>
            <name evidence="3">Transglycosylase P7</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>P27380-2</id>
            <name evidence="3">Protein P14</name>
            <sequence type="described" ref="VSP_022588"/>
        </isoform>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transglycosylase Slt family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.n1" evidence="3"/>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B36777">
        <property type="entry name" value="WMBPG7"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040700.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_001542614.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="SMR" id="P27380"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000189">
        <property type="entry name" value="Transglyc_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00922">
        <property type="entry name" value="TRANSGLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0024">Alternative initiation</keyword>
    <keyword id="KW-0929">Antimicrobial</keyword>
    <keyword id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword id="KW-1231">Capsid inner membrane protein</keyword>
    <keyword id="KW-1235">Degradation of host cell envelope components during virus entry</keyword>
    <keyword id="KW-1236">Degradation of host peptidoglycans during virus entry</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-1171">Viral genome ejection through host cell envelope</keyword>
    <keyword id="KW-1162">Viral penetration into host cytoplasm</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="initiator methionine" description="Removed; by host">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000196561" description="Transglycosylase">
        <location>
            <begin position="2"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Slt-type domain">
        <location>
            <begin position="19"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_022588" description="In isoform Protein P14." evidence="7">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10931330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="11741849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="12453208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="10931330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <sequence length="265" mass="27074" checksum="332B210FC6136F3C" modified="2007-01-23" version="3">MSGALQWWETIGAASAQYNLDPRLVAGVVQTESSGNPRTTSGVGAMGLMQLMPATAKSLGVTNAYDPTQNIYGGAALLRENLDRYGDVNTALLAYHGGTNQANWGAKTKSYPGKVMKNINLLFGNSGPVVTPAAGIAPVSGAQEMTAVNISDYTAPDLTGLTMGAGSPDFTGGASGSWGEENIPWYRVDKHVANAAGSAYDAVTDAVSAPVEAAGNYALRGVVIIAAVAIVVVGLYFLFQDEINSAAMKMIPAGKAAGAAAKALA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>P27381</accession>
    <accession>Q3T4N5</accession>
    <name>PKG9_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>DNA packaging ATPase P9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein P9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">IX</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 2-7</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="348" first="617" last="629">
            <title>In vitro DNA packaging of PRD1: a common mechanism for internal-membrane viruses.</title>
            <authorList>
                <person name="Stromsten N.J."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K.H."/>
            </authorList>
            <dbReference type="PubMed" id="15826659"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.03.002"/>
        </citation>
        <scope>IDENTIFICATION IN THE PORTAL COMPLEX</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2014" name="PLoS Biol." volume="12" first="E1002024" last="E1002024">
            <title>A structural model of the genome packaging process in a membrane-containing double stranded DNA virus.</title>
            <authorList>
                <person name="Hong C."/>
                <person name="Oksanen H.M."/>
                <person name="Liu X."/>
                <person name="Jakana J."/>
                <person name="Bamford D.H."/>
                <person name="Chiu W."/>
            </authorList>
            <dbReference type="PubMed" id="25514469"/>
            <dbReference type="DOI" id="10.1371/journal.pbio.1002024"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>IDENTIFICATION IN THE PORTAL COMPLEX</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Together with the packaging efficiency factor P6, forms the external part of the portal vertex that is embeded in the capsid and which plays critical roles in genome packaging and genome ejection. Both proteins multimerize as a single ring-shaped heterdodecamer arranged around a central channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Heterodimer of P6 and P9; further multimerizes as hexamers of heterodimers. Part of the dodecameric portal complex that is composed of the packaging efficiency factor P6, the DNA packaging ATPase P9, and the internal heterododecamer P20/P22 which spans the virion inner membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
        <text evidence="4">Component of the capsid. Localizes at the unique vertex of the capsid, which extends to the internal membrane via two small integral membrane proteins, P20 and P22.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="I40477">
        <property type="entry name" value="WMBPQ9"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040689.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="SMR" id="P27381"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019028">
        <property type="term" value="C:viral capsid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019073">
        <property type="term" value="P:viral DNA genome packaging"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CACAO"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0231">Viral genome packaging</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="initiator methionine" description="Removed; by host" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000165351" description="DNA packaging ATPase P9">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15826659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="1853567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="25514469"/>
        </source>
    </evidence>
    <sequence length="227" mass="25787" checksum="1125CCF088FC0DEB" modified="2007-01-23" version="3">MTIRMPNDRQRILVLGKTGTGKTCAAVWHLSQKDFKRKAWIVLNHKGDDLIDSIEGANHVDLNFRPKKPGLYIYHPIPDVDDAEVTQLLWDIHAMGDIGVYVDEGYMIPNRDPAFQALLTQGRSKKIPMIILSQRPVWLTRFAISESDFFQIFQLGDQRDRQTVQGFVPVDLEKLMQAPVNTVPALKKFHSIYYDVGANNCVIMTPVPTADAVLARFDLGKKRKQTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>P27382</accession>
    <accession>Q3T4M3</accession>
    <name>VP11_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Infectivity protein P11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">XI</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PARTIAL PROTEIN SEQUENCE</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2002" name="Mol. Microbiol." volume="46" first="1199" last="1209">
            <title>Sequential model of phage PRD1 DNA delivery: active involvement of the viral membrane.</title>
            <authorList>
                <person name="Grahn A.M."/>
                <person name="Daugelavicius R."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="12453208"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.2002.03250.x"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the phage ejection machinery. Pilot protein for the formation of the tube that conducts the genome into the target cell. Probably involved in penetration of the bacterial outer membrane and for making the peptidoglycan layer accessible to the viral transglycosylase. Essential for viral infectivity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Virion membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="3">Part of the capsid inner membrane.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="I36776">
        <property type="entry name" value="WMBPXB"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040698.2">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="TCDB" id="1.K.2.1.1">
        <property type="family name" value="the prd1 phage dna delivery (prd1-dd) family"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1231">Capsid inner membrane protein</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-1171">Viral genome ejection through host cell envelope</keyword>
    <keyword id="KW-1162">Viral penetration into host cytoplasm</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="chain" id="PRO_0000165353" description="Infectivity protein P11">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12453208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="207" mass="22209" checksum="D910C1BC0BF5B0D1" modified="2007-01-23" version="2">MEKVKAWLIKYKWWIVAAIGGLAAFLLLKNRGGGSGGGGEYMVGSGPVYQQAGSGAVDNTMALAALQANTQLSAQNAQLQAQMDASRLQLETQLNIETLAADNAHYSTQSQLQLGMAQVDLSKYLGDLQSTTSTALAGMQSDTAKYQSNIQLQAENIRANTSLAEIDAQKYIVGKQADIAKYQAKTERRGQDYGFALGLLNFGGKFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2022-02-23" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>P27383</accession>
    <accession>Q3T4N9</accession>
    <name>VP17_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Protein P17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">XVII</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1999" name="Eur. J. Biochem." volume="260" first="549" last="558">
            <title>Purification and characterization of the assembly factor P17 of the lipid-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Caldentey J."/>
                <person name="Hanninen A.L."/>
                <person name="Holopainen J.M."/>
                <person name="Bamford J.K.H."/>
                <person name="Kinnunen P.K."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="10095794"/>
            <dbReference type="DOI" id="10.1046/j.1432-1327.1999.00202.x"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text>Assembly protein that acts late in phage assembly, after capsid protein folding and multimerization, and sorting of membrane proteins has occurred. The major coat protein P3 and two assembly factors (P10 and P17) are needed during the assembly of the virus particle inside the host cell, when the capsid protein multimers are capable of enclosing the host-derived membrane, containing the virus-encoded membrane-associated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G40477">
        <property type="entry name" value="WMBP77"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040687.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="SMR" id="P27383"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000165355" description="Protein P17">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10095794"/>
        </source>
    </evidence>
    <sequence length="86" mass="9538" checksum="54C44109CAD67FAA" modified="1992-08-01" version="1">MMGKGFEMMVASAIRAAGINPDELMEKANTLVHNLNYQLDRFGQRLDSIDSRLSVIEKALDISPAEKPDNQPELTGITFEGDNNDQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>P27384</accession>
    <accession>Q3T4P1</accession>
    <name>VP31_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Penton protein P31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>GpC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">XXXI</name>
        <name type="synonym">C</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 28-40</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="J. Biol. Chem." volume="276" first="46187" last="46195">
            <title>Solution structure of bacteriophage PRD1 vertex complex.</title>
            <authorList>
                <person name="Sokolova A."/>
                <person name="Malfois M."/>
                <person name="Caldentey J."/>
                <person name="Svergun D.I."/>
                <person name="Koch M.H."/>
                <person name="Bamford D.H."/>
                <person name="Tuma R."/>
            </authorList>
            <dbReference type="PubMed" id="11577098"/>
            <dbReference type="DOI" id="10.1074/jbc.M106848200"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2004" name="Nature" volume="432" first="68" last="74">
            <title>Insights into assembly from structural analysis of bacteriophage PRD1.</title>
            <authorList>
                <person name="Abrescia N.G.A."/>
                <person name="Cockburn J.J.B."/>
                <person name="Grimes J.M."/>
                <person name="Sutton G.C."/>
                <person name="Diprose J.M."/>
                <person name="Butcher S.J."/>
                <person name="Fuller S.D."/>
                <person name="San Martin C."/>
                <person name="Burnett R.M."/>
                <person name="Stuart D.I."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K.H."/>
            </authorList>
            <dbReference type="PubMed" id="15525981"/>
            <dbReference type="DOI" id="10.1038/nature03056"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (4.2 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">In association with P2 and trimeric P5, forms the spike complexes located at the 5-fold vertices of the capsid. Essential for viral infectivity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E40477">
        <property type="entry name" value="WMBPQC"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040685.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="PDB" id="1W8X">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="4.20 A"/>
        <property type="chains" value="N=1-126"/>
    </dbReference>
    <dbReference type="PDBsum" id="1W8X"/>
    <dbReference type="SMR" id="P27384"/>
    <dbReference type="EvolutionaryTrace" id="P27384"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015043">
        <property type="entry name" value="Phage_PRD1_P5_spike_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08948">
        <property type="entry name" value="DUF1859"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000165357" description="Penton protein P31">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="11577098"/>
        </source>
    </evidence>
    <sequence length="126" mass="13746" checksum="B2415982C59287EC" modified="1992-08-01" version="1">MNVNNPNQMTVTPVYNGCDSGEGPQSVRGYFDAVAGENVKYDLTYLADTQGFTGVQCIYIDNAENDGAFEIDVEETGQRIKCPAGKQGYFPLLVPGRAKFVARHLGSGKKSVPLFFLNFTIAQGVW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2022-02-23" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>P27386</accession>
    <accession>Q3T4N4</accession>
    <name>YPI_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Putative uncharacterized 4.5 kDa protein in genes IX-XX intergenic region</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>ORFi</fullName>
        </alternativeName>
    </protein>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A36776">
        <property type="entry name" value="WMBPPI"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040690.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="SMR" id="P27386"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000165364" description="Putative uncharacterized 4.5 kDa protein in genes IX-XX intergenic region">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="42" mass="4541" checksum="B89A83DED4FFA8FA" modified="1992-08-01" version="1">MTENIISWNVANWVTVILMALAGYAVLALAAKVINNRAQEPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>P27387</accession>
    <accession>Q3T4N3</accession>
    <name>PKG20_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Packaging protein P20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">XX</name>
        <name type="synonym">J</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="J. Virol." volume="77" first="6314" last="6321">
            <title>The unique vertex of bacterial virus PRD1 is connected to the viral internal membrane.</title>
            <authorList>
                <person name="Stromsten N.J."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K.H."/>
            </authorList>
            <dbReference type="PubMed" id="12743288"/>
            <dbReference type="DOI" id="10.1128/JVI.77.11.6314-6321.2003"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>IDENTIFICATION IN THE PORTAL COMPLEX</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2014" name="PLoS Biol." volume="12" first="E1002024" last="E1002024">
            <title>A structural model of the genome packaging process in a membrane-containing double stranded DNA virus.</title>
            <authorList>
                <person name="Hong C."/>
                <person name="Oksanen H.M."/>
                <person name="Liu X."/>
                <person name="Jakana J."/>
                <person name="Bamford D.H."/>
                <person name="Chiu W."/>
            </authorList>
            <dbReference type="PubMed" id="25514469"/>
            <dbReference type="DOI" id="10.1371/journal.pbio.1002024"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>IDENTIFICATION IN THE PORTAL COMPLEX</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Together with P22, forms the internal part of the portal complex embeded in the virion internal membrane and which plays critical roles in genome packaging and genome ejection. Both proteins multimerize as a single ring-shaped heterdodecamer arranged around a central channel and interact with the P6/P9 external part of the portal.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Heterodimer of P20 and P22; further multimerizes as hexamers of heterodimers. Part of the dodecameric portal complex that is composed of the packaging efficiency factor P6, the DNA packaging ATPase P9, and the internal heterododecamer P20/P22 which spans the virion inner membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion membrane</location>
            <topology>Single-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="2">Part of the capsid inner membrane. Binds the portal complex at the capsid vertex.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B36776">
        <property type="entry name" value="WMBPPJ"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040691.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="SMR" id="P27387"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039641">
        <property type="term" value="C:viral inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055036">
        <property type="term" value="C:virion membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1231">Capsid inner membrane protein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0231">Viral genome packaging</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000165365" description="Packaging protein P20">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12743288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="25514469"/>
        </source>
    </evidence>
    <sequence length="42" mass="4671" checksum="E1BB890A4897A3EE" modified="1992-08-01" version="1">MVNWELLKNPINWLIVILMLTIAGMAATLVCNHFGKNAVTSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2022-02-23" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>P27388</accession>
    <accession>Q3T4N1</accession>
    <name>PKG22_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Packaging protein P22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>GpK</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Protein K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">XXII</name>
        <name type="synonym">K</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 1-9</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="J. Virol." volume="77" first="6314" last="6321">
            <title>The unique vertex of bacterial virus PRD1 is connected to the viral internal membrane.</title>
            <authorList>
                <person name="Stromsten N.J."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K.H."/>
            </authorList>
            <dbReference type="PubMed" id="12743288"/>
            <dbReference type="DOI" id="10.1128/JVI.77.11.6314-6321.2003"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>IDENTIFICATION IN THE PORTAL COMPLEX</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2014" name="PLoS Biol." volume="12" first="E1002024" last="E1002024">
            <title>A structural model of the genome packaging process in a membrane-containing double stranded DNA virus.</title>
            <authorList>
                <person name="Hong C."/>
                <person name="Oksanen H.M."/>
                <person name="Liu X."/>
                <person name="Jakana J."/>
                <person name="Bamford D.H."/>
                <person name="Chiu W."/>
            </authorList>
            <dbReference type="PubMed" id="25514469"/>
            <dbReference type="DOI" id="10.1371/journal.pbio.1002024"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>IDENTIFICATION IN THE PORTAL COMPLEX</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Together with P22, forms the internal part of the portal complex embeded in the virion internal membrane and which plays critical roles in genome packaging and genome ejection. Both proteins multimerize as a single ring-shaped heterdodecamer arranged around a central channel and interact with the P6/P9 external part of the portal.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Heterodimer of P20 and P22; further multimerizes as hexamers of heterodimers. Part of the dodecameric portal complex that is composed of the packaging efficiency factor P6, the DNA packaging ATPase P9, and the internal heterododecamer P20/P22 which spans the virion inner membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion membrane</location>
            <topology>Single-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="2">Part of the capsid inner membrane. Binds the portal complex at the capsid vertex.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E36776">
        <property type="entry name" value="WMBPKB"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040694.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="SMR" id="P27388"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1231">Capsid inner membrane protein</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0231">Viral genome packaging</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000165359" description="Packaging protein P22">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12743288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="25514469"/>
        </source>
    </evidence>
    <sequence length="47" mass="5493" checksum="58649E1BAAEEBAAA" modified="1992-08-01" version="1">MQLITDMAEWSSKPFRPDMSLTGWLAFVGLIIVAIILWQQIIRFIIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2022-02-23" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>P27389</accession>
    <accession>Q3T4M8</accession>
    <name>VP18_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Protein P18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>GpM</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Protein M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">XVIII</name>
        <name type="synonym">M</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2002" name="Mol. Microbiol." volume="46" first="1199" last="1209">
            <title>Sequential model of phage PRD1 DNA delivery: active involvement of the viral membrane.</title>
            <authorList>
                <person name="Grahn A.M."/>
                <person name="Daugelavicius R."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="12453208"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.2002.03250.x"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the phage injection machinery. Required for DNA injection in the membrane transformation event. Involved in the formation of the membrane tail tube to connect the virus interior with the host cytosol. Essential for viral infectivity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Virion membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="3">Part of the capsid inner membrane.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F36776">
        <property type="entry name" value="WMBPMB"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040695.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="TCDB" id="1.K.2.1.1">
        <property type="family name" value="the prd1 phage dna delivery (prd1-dd) family"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1231">Capsid inner membrane protein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-1171">Viral genome ejection through host cell envelope</keyword>
    <keyword id="KW-1162">Viral penetration into host cytoplasm</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="chain" id="PRO_0000165360" description="Protein P18">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12453208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="90" mass="9789" checksum="0CE80254B79683E4" modified="1992-08-01" version="1">MPFGLIVIGIILAIAAYRDTLGELFSIIKDVSKDAKGFGYWVLAAVILGFAASIKPIKEPVNAFMILLMIVLLIRKRGAIDQISNQLRGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2022-02-23" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>P27390</accession>
    <accession>Q3T4M6</accession>
    <name>VP34_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Protein P34</fullName>
            <shortName>Protein O</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>GpO</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">XXXIV</name>
        <name type="synonym">O</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 61-68</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="2">Part of the capsid inner membrane.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G36776">
        <property type="entry name" value="WMBPUB"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040696.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="SMR" id="P27390"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020085">
        <property type="entry name" value="DNA_delivery_prot_P32/34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11087">
        <property type="entry name" value="PRD1_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1231">Capsid inner membrane protein</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000165361" description="Protein P34">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="68" mass="6695" checksum="86E351A8AF13746A" modified="1992-08-01" version="1">MNDFVGPIVTVLTAIIGVAILAVLVSRNSNTAGVIKAGSGGFSSMLGTALSPVTGGTGFAMTNNYSGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>P27391</accession>
    <accession>Q3T4M5</accession>
    <name>VP30_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Minor capsid protein P30</fullName>
            <shortName>Protein P</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>GpP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">XXX</name>
        <name type="synonym">P</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 2-10</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="J. Mol. Biol." volume="313" first="785" last="795">
            <title>A minor capsid protein P30 is essential for bacteriophage PRD1 capsid assembly.</title>
            <authorList>
                <person name="Rydman P.S."/>
                <person name="Bamford J.K.H."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="11697904"/>
            <dbReference type="DOI" id="10.1006/jmbi.2001.5068"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2004" name="Nature" volume="432" first="68" last="74">
            <title>Insights into assembly from structural analysis of bacteriophage PRD1.</title>
            <authorList>
                <person name="Abrescia N.G.A."/>
                <person name="Cockburn J.J.B."/>
                <person name="Grimes J.M."/>
                <person name="Sutton G.C."/>
                <person name="Diprose J.M."/>
                <person name="Butcher S.J."/>
                <person name="Fuller S.D."/>
                <person name="San Martin C."/>
                <person name="Burnett R.M."/>
                <person name="Stuart D.I."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K.H."/>
            </authorList>
            <dbReference type="PubMed" id="15525981"/>
            <dbReference type="DOI" id="10.1038/nature03056"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (4.2 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Minor capsid protein essential for stable capsid assembly of complete particles.</text>
    </comment>
    <comment type="subunit">
        <text>Dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
        <text>The capsid contains 60 copies of P30.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H36776">
        <property type="entry name" value="WMBPWB"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040697.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="PDB" id="1W8X">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="4.20 A"/>
        <property type="chains" value="M=2-84"/>
    </dbReference>
    <dbReference type="PDBsum" id="1W8X"/>
    <dbReference type="SMR" id="P27391"/>
    <dbReference type="EvolutionaryTrace" id="P27391"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015379">
        <property type="entry name" value="Tecti-min-caps"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09300">
        <property type="entry name" value="Tecti-min-caps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="initiator methionine" description="Removed; by host" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000165362" description="Minor capsid protein P30">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="11697904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="1853567"/>
        </source>
    </evidence>
    <sequence length="84" mass="9187" checksum="1F81BAAFDF197943" modified="2007-01-23" version="3">MALINPQFPYAGPVPIPGPAPTETMPLLNYRVEGRIAGIQQARQFMPFLQGPHRAVAEQTYHAIGTGIQMGQTFNQPLINTQEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>P27392</accession>
    <accession>Q3T4M2</accession>
    <name>VP16_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Protein P16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>GpS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Protein S</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">XVI</name>
        <name type="synonym">S</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PARTIAL PROTEIN SEQUENCE</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2004" name="J. Virol." volume="78" first="9790" last="9797">
            <title>Integral membrane protein P16 of bacteriophage PRD1 stabilizes the adsorption vertex structure.</title>
            <authorList>
                <person name="Jaatinen S.T."/>
                <person name="Viitanen S.J."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K.H."/>
            </authorList>
            <dbReference type="PubMed" id="15331712"/>
            <dbReference type="DOI" id="10.1128/JVI.78.18.9790-9797.2004"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2004" name="Nature" volume="432" first="68" last="74">
            <title>Insights into assembly from structural analysis of bacteriophage PRD1.</title>
            <authorList>
                <person name="Abrescia N.G.A."/>
                <person name="Cockburn J.J.B."/>
                <person name="Grimes J.M."/>
                <person name="Sutton G.C."/>
                <person name="Diprose J.M."/>
                <person name="Butcher S.J."/>
                <person name="Fuller S.D."/>
                <person name="San Martin C."/>
                <person name="Burnett R.M."/>
                <person name="Stuart D.I."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K.H."/>
            </authorList>
            <dbReference type="PubMed" id="15525981"/>
            <dbReference type="DOI" id="10.1038/nature03056"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (4.2 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Protein of the infection vertex complex, which increases the vertex stability. Anchors the vertex structure to the viral membrane. Essential for viral infectivity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Virion membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="3">Part of the capsid inner membrane.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A36777">
        <property type="entry name" value="WMBPSB"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_040699.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="PDB" id="1W8X">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="4.20 A"/>
        <property type="chains" value="P=1-117"/>
    </dbReference>
    <dbReference type="PDBsum" id="1W8X"/>
    <dbReference type="SMR" id="P27392"/>
    <dbReference type="EvolutionaryTrace" id="P27392"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="DisProt" id="DP01012"/>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1231">Capsid inner membrane protein</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000165363" description="Protein P16">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15331712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="117" mass="12551" checksum="0995B6CBD35BDE7C" modified="1992-08-01" version="1">MDKKKLLYWVGGGLVLILIWLWFRNRPAAQVASNWEGPPYMTYNQPQAGSVTLPVAGYTSPSPTLPNRNRSCGCNPAVSAAMAQGADLASKLTDSITSQLNDYASSLNDYLASQAGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-12-01" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>P28732</accession>
    <accession>Q3T4N6</accession>
    <name>VP10_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Protein P10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">X</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text>Assembly protein. The major coat protein P3 and two assembly factors (P10 and P17) are needed during the assembly of the virus particle inside the host cell, when the capsid protein multimers are capable of enclosing the host-derived membrane, containing the virus-encoded membrane-associated proteins.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E36777">
        <property type="entry name" value="WMBPTB"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_001542609.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0118">Viral capsid assembly</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <feature type="chain" id="PRO_0000165352" description="Protein P10">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="203" mass="20688" checksum="064C2BB9C08151D9" modified="1992-12-01" version="1">MAGKSSNGDGVKFADDAGSGNSIDGIAIIDPGTVTSASGGSGSDAGSGDSGNTPRKRGRPAGSGTGAKRGRPAGNSAAKKVPGNLGVEKILFSLHLMASSALKVEELALDEKEAEALAGAVQEVASHYDITPDPKIMAWAGLFGVCASIYGPRVAAYKMRAAAEKRDKKEAAKPMAQKVQEAKPTEHDPAAPYIEQLPVSNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2020-08-12" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q3T4L7</accession>
    <name>Q3T4L7_BPPRD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein ORFv</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ORFv</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_001542617.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAX45901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002143"/>
        </source>
    </evidence>
    <sequence length="90" mass="10230" checksum="FFF5EDDACEADE3F5" modified="2005-10-11" version="1">MKLNKFLIVLCLPMFAACSTTAPKIETVYLVPPSSLLTECAAPVYPFITWRDLVEAYAKEKAARESCGLQIQEIKNWFKETVPSESRKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2020-08-12" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q3T4L8</accession>
    <name>Q3T4L8_BPPRD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein ORFu</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ORFu</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_001542616.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAX45900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002143"/>
        </source>
    </evidence>
    <sequence length="120" mass="12711" checksum="3EADDE689FD9168C" modified="2005-10-11" version="1">MIKWVLSNLKSVAAIAVALLMLANFGYSILAAKKITGLQNEIKAIEEKATANAKAVETLQSVRASDAKAIAAIQEGQTIISGKLSRTISGLGRVSDETKQILDSPMPADVRGLFDNSPEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3T4L9</accession>
    <name>HOLIN_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Holin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein P35</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">XXXV</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="J. Bacteriol." volume="185" first="3795" last="3803">
            <title>Identification and mutational analysis of bacteriophage PRD1 holin protein P35.</title>
            <authorList>
                <person name="Rydman P.S."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="12813073"/>
            <dbReference type="DOI" id="10.1128/JB.185.13.3795-3803.2003"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>MUTAGENESIS</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2005" name="J. Bacteriol." volume="187" first="5397" last="5405">
            <title>The holin protein of bacteriophage PRD1 forms a pore for small-molecule and endolysin translocation.</title>
            <authorList>
                <person name="Ziedaite G."/>
                <person name="Daugelavicius R."/>
                <person name="Bamford J.K.H."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="16030234"/>
            <dbReference type="DOI" id="10.1128/JB.187.15.5397-5405.2005"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 4 5">Isoform Holin: Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space (PubMed:12813073, PubMed:16030234). Determines the precise timing of host cell lysis (By similarity). Participates with the endolysin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles from the host cell.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Isoform Antiholin: Counteracts the aggregation of the holin molecules and thus of pore formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homomultimer. Isoform Holin: Interacts with isoform Antiholin; this interaction blocks the holin homomultimerization and delays host cell lysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="6">Classified as a class I holin.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_001542615.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="TCDB" id="1.E.5.1.1">
        <property type="family name" value="the prd1 phage p35 holin (p35 holin) family"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032126">
        <property type="entry name" value="LydA_holin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16083">
        <property type="entry name" value="Phage_holin_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0929">Antimicrobial</keyword>
    <keyword id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword id="KW-0204">Cytolysis</keyword>
    <keyword id="KW-1030">Host cell inner membrane</keyword>
    <keyword id="KW-0578">Host cell lysis by virus</keyword>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <feature type="chain" id="PRO_0000234092" description="Holin">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P03705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="12813073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="16030234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="117" mass="12778" checksum="F73511CBE428EDC2" modified="2005-10-11" version="1">MMENDEWWKYLIFPLVATLGGIVNYSKRALAMKRFSKLEFAVEAVSAAFVGLMVTLGGAAMDLSPHWLGMAAGMSGWMGADFVKAVFSQFVQSKIAPINQPGPIDSDNDKPGRTFND</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2020-08-12" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q3T4M4</accession>
    <name>Q3T4M4_BPPRD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein ORFq</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ORFq</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_001542613.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAX45899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002143"/>
        </source>
    </evidence>
    <sequence length="36" mass="4153" checksum="28EDA9B3A7FF20C4" modified="2005-10-11" version="1">MKTAIFILAVMFAALLWLGREHCILCQWFKRISNGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2020-10-07" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3T4M7</accession>
    <name>VP32_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Protein P32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">XXXII</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2002" name="Mol. Microbiol." volume="46" first="1199" last="1209">
            <title>Sequential model of phage PRD1 DNA delivery: active involvement of the viral membrane.</title>
            <authorList>
                <person name="Grahn A.M."/>
                <person name="Daugelavicius R."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="12453208"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.2002.03250.x"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2002" name="J. Virol." volume="76" first="4866" last="4872">
            <title>The small viral membrane-associated protein P32 is involved in bacteriophage PRD1 DNA entry.</title>
            <authorList>
                <person name="Grahn A.M."/>
                <person name="Daugelavicius R."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="11967303"/>
            <dbReference type="DOI" id="10.1128/JVI.76.10.4866-4872.2002"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Component of the phage injection machinery. Required for DNA injection in the membrane transformation event. Involved in the formation of the membrane tail tube to connect the virus interior with the host cytosol. Essential for viral infectivity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Virion membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="4">Part of the capsid inner membrane.</text>
    </comment>
    <comment type="miscellaneous">
        <text>PRD1 virions are composed of a tail-less icosahedral capsid of diameter 63 nm, an inner protein-lipid membrane, and a dsDNA genome which is located inside the lipid vesicle. The DNA is packaged into a preformed procapsid. The internal membrane plays an active role in DNA delivery to the host cell by forming a tubular structure used for injecting the DNA into the host cytoplasm.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_001542612.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q3T4M7"/>
    <dbReference type="TCDB" id="1.K.2.1.1">
        <property type="family name" value="the prd1 phage dna delivery (prd1-dd) family"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020085">
        <property type="entry name" value="DNA_delivery_prot_P32/34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11087">
        <property type="entry name" value="PRD1_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1231">Capsid inner membrane protein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-1171">Viral genome ejection through host cell envelope</keyword>
    <keyword id="KW-1162">Viral penetration into host cytoplasm</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="chain" id="PRO_0000234090" description="Protein P32">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11967303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12453208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="54" mass="5457" checksum="A640CC34F11B8A55" modified="2005-10-11" version="1">MGEFGKTLITIVTAIIGVAIIAVIVSQRSNTAGVIQSATSGFSNILKSALAPII</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2020-08-12" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q3T4N0</accession>
    <name>Q3T4N0_BPPRD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein ORFx</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ORFx</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_001542610.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAX45902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002143"/>
        </source>
    </evidence>
    <sequence length="27" mass="2974" checksum="0A7EDD984B21E054" modified="2005-10-11" version="1">MKIFGISVFTIAIIALAYYLGTKRIIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2022-02-23" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>Q3T4N8</accession>
    <name>VP33_BPPRD</name>
    <protein>
        <recommendedName>
            <fullName>Protein P33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">XXXIII</name>
    </gene>
    <organism>
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text>Assembly protein.</text>
    </comment>
    <comment type="miscellaneous">
        <text>PRD1 virions are composed of a tail-less icosahedral capsid of diameter 63 nm, an inner protein-lipid membrane, and a dsDNA genome which is located inside the lipid vesicle. The DNA is packaged into a preformed procapsid. The internal membrane plays an active role in DNA delivery to the host cell by forming a tubular structure used for injecting the DNA into the host cytoplasm.</text>
    </comment>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_001542608.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q3T4N8"/>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000234091" description="Protein P33">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="68" mass="7451" checksum="09BB00AC23B2B7BE" modified="2005-10-11" version="1">MTNEEKLIAEIDSAGLSAEMLESMPSPEMFPLIIVNLQGRIAELEARETEMLARVDTLIARLAHLGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-08-16" modified="2020-08-12" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q6EDW7</accession>
    <accession>Q3T4M9</accession>
    <accession>Q3T4R0</accession>
    <name>Q6EDW7_BPPRD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein ORFl</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ORFl</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Enterobacteria phage PRD1</name>
        <name type="synonym">Bacteriophage PRD1</name>
        <dbReference type="NCBI Taxonomy" id="10658"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Varidnaviria</taxon>
            <taxon>Bamfordvirae</taxon>
            <taxon>Preplasmiviricota</taxon>
            <taxon>Tectiliviricetes</taxon>
            <taxon>Kalamavirales</taxon>
            <taxon>Tectiviridae</taxon>
            <taxon>Alphatectivirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Pseudomonas aeruginosa</name>
        <dbReference type="NCBI Taxonomy" id="287"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas fluorescens</name>
        <dbReference type="NCBI Taxonomy" id="294"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Pseudomonas putida</name>
        <name type="synonym">Arthrobacter siderocapsulatus</name>
        <dbReference type="NCBI Taxonomy" id="303"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Acinetobacter calcoaceticus</name>
        <dbReference type="NCBI Taxonomy" id="471"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Salmonella typhimurium</name>
        <dbReference type="NCBI Taxonomy" id="90371"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Escherichia coli</name>
        <dbReference type="NCBI Taxonomy" id="562"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Proteus mirabilis</name>
        <dbReference type="NCBI Taxonomy" id="584"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Vibrio cholerae</name>
        <dbReference type="NCBI Taxonomy" id="666"/>
    </organismHost>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="1991" name="Virology" volume="183" first="658" last="676">
            <title>Genome organization of membrane-containing bacteriophage PRD1.</title>
            <authorList>
                <person name="Bamford J.K."/>
                <person name="Hanninen A.L."/>
                <person name="Pakula T.M."/>
                <person name="Ojala P.M."/>
                <person name="Kalkkinen N."/>
                <person name="Frilander M."/>
                <person name="Bamford D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1853567"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90995-N"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="427" last="440">
            <title>A snapshot of viral evolution from genome analysis of the tectiviridae family.</title>
            <authorList>
                <person name="Saren A.M."/>
                <person name="Ravantti J.J."/>
                <person name="Benson S.D."/>
                <person name="Burnett R.M."/>
                <person name="Paulin L."/>
                <person name="Bamford D.H."/>
                <person name="Bamford J.K."/>
            </authorList>
            <dbReference type="PubMed" id="15946683"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.059"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <dbReference type="EMBL" id="AY848689">
        <property type="protein sequence ID" value="AAX45898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_001542611.1">
        <property type="nucleotide sequence ID" value="NC_001421.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002143">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAX45898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002143"/>
        </source>
    </evidence>
    <sequence length="40" mass="4399" checksum="A075E7BCDC91720E" modified="2004-08-16" version="1">MRGNLALAMILFGFVVYAANSGAFGRLLYVIGFRDKPEGY</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>