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<entry dataset="TrEMBL" created="2017-03-15" modified="2021-04-07" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>A0A1P7U1I1</accession>
    <name>A0A1P7U1I1_HCMVM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane glycoprotein UL8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">UL8</name>
        <name evidence="2" type="ORF">HHV5wtgp014</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2003" name="J. Gen. Virol." volume="84" first="657" last="663">
            <title>Homology between the human cytomegalovirus RL11 gene family and human adenovirus E3 genes.</title>
            <authorList>
                <person name="Davison A.J."/>
                <person name="Akter P."/>
                <person name="Cunningham C."/>
                <person name="Dolan A."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Wilkinson G.W."/>
            </authorList>
            <dbReference type="PubMed" id="12604818"/>
            <dbReference type="DOI" id="10.1099/vir.0.18856-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Merlin</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2003" name="J. Gen. Virol." volume="84" first="1117" last="1122">
            <title>Two novel spliced genes in human cytomegalovirus.</title>
            <authorList>
                <person name="Akter P."/>
                <person name="Cunningham C."/>
                <person name="McSharry B.P."/>
                <person name="Dolan A."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="12692276"/>
            <dbReference type="DOI" id="10.1099/vir.0.18952-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Merlin</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="3">
        <citation type="journal article" date="2003" name="J. Gen. Virol." volume="84" first="2511" last="2516">
            <title>The most abundantly transcribed human cytomegalovirus gene (beta 2.7) is non-essential for growth in vitro.</title>
            <authorList>
                <person name="McSharry B.P."/>
                <person name="Tomasec P."/>
                <person name="Neale M.L."/>
                <person name="Wilkinson G.W."/>
            </authorList>
            <dbReference type="PubMed" id="12917473"/>
            <dbReference type="DOI" id="10.1099/vir.0.19298-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Merlin</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="4">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Merlin</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31574.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081468.2">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="DNASU" id="3077499"/>
    <dbReference type="GeneID" id="3077499"/>
    <dbReference type="KEGG" id="vg:3077499"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="SSF48726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAR31574.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000938"/>
        </source>
    </evidence>
    <sequence length="324" mass="35743" checksum="FB3675A3C0B79AE2" modified="2017-03-15" version="1">MASDVGSHPLTVTRFRCRVHYVYNKLLILTLFAPVILESVIYVSGPQGGNVTLVSNFTSNISARWFRWDGNDSHLICFYKRGEGLSTPYVGLSLSCAANQITIFNLTLNDSGRYGAEGFTRSGENETFLWYNLTVKPKPLETTPASNVTTIVTTTSTVTDAKSNVTGNVSLAPQLRAVAGFSHQTPLENNTHLALGEGFVPTMTSSRLSASENYDGNYEFTETANTTRTNTSDWITLGSSASLLKSTETAVNLSNATTVIPQPVEYPAGGVQYQRAATHYSWMLIIVIILIIFIIICLRAPRKIYHHWKDSKQYGQVFMTDTEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5H8R0</accession>
    <name>U147A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL147A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL147A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081569.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5H8R0"/>
    <dbReference type="DNASU" id="3077569"/>
    <dbReference type="GeneID" id="3077569"/>
    <dbReference type="KEGG" id="vg:3077569"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033644">
        <property type="term" value="C:host cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418260" description="Uncharacterized protein UL147A">
        <location>
            <begin position="26"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="75" mass="8291" checksum="8BCF27F868104C1B" modified="2011-06-28" version="1" precursor="true">MSLFYRAVALGTLSALVWYSTSILAEINENSCSSSSVDHEDCEEPDEIVREEQDYRALLAFSLVICGTLLVTCVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F5H8R6</accession>
    <name>UL96_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL96</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL96</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae UL96 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081543.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5H8R6"/>
    <dbReference type="DNASU" id="3077498"/>
    <dbReference type="GeneID" id="3077498"/>
    <dbReference type="KEGG" id="vg:3077498"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022614">
        <property type="entry name" value="Herpesvirus_UL96"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10867">
        <property type="entry name" value="DUF2664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000418319" description="Protein UL96">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="60"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="127" mass="14465" checksum="8C61D317FC407275" modified="2011-06-28" version="1">MTSVNKQLLKDVMRVDLERQQHQFLRRTYGPQHRLTTQQALTVMRVAAREQTRYSQRTTQCVAAHLLEQRAAVQQELQRARQLQSGNVDDALDSLTELKDTVDDVRATLVDSVSATCDLDLEVDDAV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F5H991</accession>
    <name>US26_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein US26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US26</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae US22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081610.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5H991"/>
    <dbReference type="GeneID" id="3077545"/>
    <dbReference type="KEGG" id="vg:3077545"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418324" description="Protein US26">
        <location>
            <begin position="1"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="603" mass="69953" checksum="0F31C31C3616FD31" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F5H9F9</accession>
    <name>UL88_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL88</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL88</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in incorporating a subset of tegument proteins into the virion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with UL48.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Accumulates in a juxtanuclear location reminiscent of the viral cytoplasmic assembly compartment.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae U59/UL88 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081536.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BioGRID" id="1678062">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="DNASU" id="3077509"/>
    <dbReference type="GeneID" id="3077509"/>
    <dbReference type="KEGG" id="vg:3077509"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072517">
        <property type="term" value="C:host cell viral assembly compartment"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007616">
        <property type="entry name" value="Herpes_U59/UL88"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04529">
        <property type="entry name" value="Herpes_U59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000418289" description="Protein UL88">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="429" mass="47689" checksum="9D1AAA685AAABD08" modified="2011-06-28" version="1">MMEAAAAAAAAFRPEERPTPGWHDAALLMDDGTVREHAFRNGPLSQLIRRVLPPPPDAEDDVVFASELCFYCSGRFNRRSSVFSIYWQKHSDLVYALTGITHCAKLVVECGQLGSSRLRWRDGDASGEERRGDDDSRDELYDVPGIYMIRVNDGGSTGPRHVIWPGTSVLWAPDVVITTVQRRISAARALVNTFRQYFFLLERRSHEELVLCPPEMEERLAPLLQSATRGDSDMFDGVVASAYHRLRMSNIPRSSARLLEHCVGLAGAKKLLLLDVPRLENYFLCQVCLYELDEDEMGEEMLGMLAGKPEDAAVSGASGGFLLHRKTMKLAACLCLLLNSLHLHQEALEALDPPPPRVEENDLVNVVLRRYYRSHGGVQARTLAAARALLADYAETFSPLGSFTRLGYDRLVSADAGVSRRHLVALLRA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5H9N4</accession>
    <name>VP22_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Tegument protein UL24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL24</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2007-08" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Davison A.J."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae US22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31589.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081483.2">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="DNASU" id="3077489"/>
    <dbReference type="GeneID" id="3077489"/>
    <dbReference type="KEGG" id="vg:3077489"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072517">
        <property type="term" value="C:host cell viral assembly compartment"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000418280" description="Tegument protein UL24">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="358" mass="40186" checksum="A0184BE082937DAE" modified="2014-02-19" version="2">MEETRAGRYIVRAGSGGQTEDTASGAESVLATLAAVRTRRRSVVCDGPPGSPTDSARHMSDLASLALTAEFGLGCLEAYVRINAGQVLPVVWPPGWNLVLQEIETDEDFKPEDVKAWSHYLCCQTRLAFVGRFVNEGVLSPDQQKKTAVCLISDEGYVFCYVREDTAVYYLARNLMEFARVGLRAVETLHCMRYLTSSLVKRYFRPLLRAWSLGLDTMARFIIRHHGQFMPLTYPPGTELRLCNLRCFENSVEGGHLLRNIKTAFGMRVLGLGTVSLKGENAPFPHLRWPVDLIPIVVAYTGAVYACDVRDDRYIRVGDNLNTFMCLGLNLLFENRRFSGHNGIYDRVPDCPKGRQHR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F5H9N9</accession>
    <name>US17_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein US17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US17</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081602.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="GeneID" id="3077567"/>
    <dbReference type="KEGG" id="vg:3077567"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033644">
        <property type="term" value="C:host cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418294" description="Protein US17">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="293" mass="31909" checksum="A261D7DD1F872E2C" modified="2011-06-28" version="1">MSPNSEATGTAWAPPPPRPSRGVIMISSVSTNDVRRFLLCMRVYSTVAVQGTCTFLLCLGLVLAFPHLKGTVFLCCTGFMPPLSLMVPTICLALLHGKRDEGSFTSPPSPGLLTIYSVLTTLSVIVASACSSSTLVTFSGLLACVLFSLCSCVTGLAGHNHRRWQVIVTLFVIGVIAFLIALYLQPVPLGHKLFLGYYAMALSFMLVVTVFDTTRLFEIAWSEADLLTLCLYENLVYLYLLILILFTTEDSLDKLIAWMTWLSSRATGATNAASISGCDLLREVQRNLTRTMA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5H9T4</accession>
    <name>UL09_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane glycoprotein UL9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL9</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HHV-5 UL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081469.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="DNASU" id="3077459"/>
    <dbReference type="GeneID" id="3077459"/>
    <dbReference type="KEGG" id="vg:3077459"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033644">
        <property type="term" value="C:host cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416712" description="Membrane glycoprotein UL9">
        <location>
            <begin position="21"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="233" mass="26998" checksum="A4FF47905792261D" modified="2011-06-28" version="1" precursor="true">MSKRLQVFPWITILFYTSKSQYWNYMTIPCTPTVGYGSHNISLHPLNNSLFQDDVFEWYIDKPMVTNKLCLYQSNERIKSNLDSPNIMWQCTDNRTLILMNLTTTYSRNYYFQSFKYLGQGVPKPNNLCYNVSVHFTHQTHCHTTTSSLYPPTSVHDSLEISQSFTSTNFTHTAVHYAAGNVEAQHDTATSHTMWIIPLVIVITIIVLICFKFPQKAWNKFTQYRYSGMLAAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5H9Z4</accession>
    <name>UL20_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane protein UL20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL20</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">Does not reach the host cell surface but is rapidely targeted to endosomes and lysosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 UL20 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081479.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="DNASU" id="3077434"/>
    <dbReference type="GeneID" id="3077434"/>
    <dbReference type="KEGG" id="vg:3077434"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044167">
        <property type="term" value="C:host cell endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035142">
        <property type="entry name" value="UL20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17582">
        <property type="entry name" value="UL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418246" description="Membrane protein UL20">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="26"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="245"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="338" mass="38330" checksum="A7FF32248B36C116" modified="2011-06-28" version="1">MLGIRAMLVMLDYYWIQLITNNGTRSNNTDTIFVSLLTGPNGVTRTAIGGLYSNYTNLTGAFGFTSTNMSATNSSAEDNWSVTNLTESCINRGESYVTTIWLLDCTKNDTYWYYGNAYNHTCEGTISGYLLGMCKLWKSWVNNITSYNTVRVESLGNETRCMLLPRQYTLNATVEWYNKSEGDVPEEFMDYVILTPLAVLTCGLQEAYILDKGRRYMYLFSVSCVGITGTVSIILVSLSLLILICYYRCGRLLICPRGFELLPEFTEEEEEKEKLLTHNDIEVQVPIRTRRLLVPWIRESKMWVLPPPLPPRPPHLIEFPPSPPPSPGPMHMVVCMPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-02-23" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F5HA06</accession>
    <name>UL147_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Putative viral CXC chemokine 2</fullName>
            <shortName>vCXCL2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL147</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the intercrine alpha (chemokine CxC) family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081570.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HA06"/>
    <dbReference type="PRIDE" id="F5HA06"/>
    <dbReference type="DNASU" id="3077419"/>
    <dbReference type="GeneID" id="3077419"/>
    <dbReference type="KEGG" id="vg:3077419"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00273">
        <property type="entry name" value="Chemokine_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033899">
        <property type="entry name" value="CXC_Chemokine_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="SSF54117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000416443" description="Putative viral CXC chemokine 2">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="52"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="159" mass="18718" checksum="A88906C486B1C542" modified="2011-06-28" version="1">MLLTWLHHPILNSRIKLLSVRYLSLTAYMLLAICPIAVRLLELEDYDKRCRCNNQILLNTLPVGTELLKPIAASESCNRQEVLAILKDKGTKCLNPNAQAVRRHINRLFFRLVLDEEQRIYDVVSTNIEFGAWPVPTAYKAFLWKYAKKLNYHYFRLRW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5HA93</accession>
    <name>TRX1_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Triplex capsid protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">TRX1</name>
        <name type="ordered locus">UL46</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Structural component of the T=16 icosahedral capsid. The capsid is composed of pentamers and hexamers of major capsid protein/MCP, which are linked together by heterotrimers called triplexes. These triplexes are formed by a single molecule of triplex protein 1/TRX1 and two copies of triplex protein 2/TRX2. Additionally, TRX1 is required for efficient transport of TRX2 to the nucleus, which is the site of capsid assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with TRX2, MCP and capsid vertex component 2/CVC2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae TRX1 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081504.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HA93"/>
    <dbReference type="PRIDE" id="F5HA93"/>
    <dbReference type="GeneID" id="3077431"/>
    <dbReference type="KEGG" id="vg:3077431"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04018">
        <property type="entry name" value="HSV_TRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004999">
        <property type="entry name" value="Herpes_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03327">
        <property type="entry name" value="Herpes_VP19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000418256" description="Triplex capsid protein 1">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04018"/>
        </source>
    </evidence>
    <sequence length="290" mass="33027" checksum="ADB19CE379503D17" modified="2011-06-28" version="1">MDARAVAKRPRDPADEDNELVTALKAKREVNTISVRYLYHADHQALTARFFVPEGLVEFEAQPGALLIRMETGCDSPRHLYISLYLLGIRASNVSASTRCLLESVYTASAARAALQWLDLGPHLLHRRLETLGCVKTVSLGITSLLTCVMRGYLYNTLKTEVFALMIPKDMYLTWEETRGRLQYVYLIIVYDYDGPETRPGIYVLTSSIAHWQTLVDVARGKFARERCSFVNRRITRPRQIPLCTGVIQKLGWCLADDIHTSFLVHKELKLSVVRLDNFSVELGDFREFV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5HAC7</accession>
    <name>CEP2_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytoplasmic envelopment protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL94</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Plays a critical role in cytoplasmic virus egress. Participates in the final step of tegumentation and envelope acquisition within the host cytoplasm by directly interacting with the capsid. Upon virion binding to target cell, a signaling cascade is triggered to disrupt the interaction with the capsid, thereby preparing capsid uncoating (By similarity). Additionally, antagonizes the viral DNA-triggered antiviral immune response by targeting host STING1 and preventing its dimerization and trafficking.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Interacts with cytoplasmic envelopment protein 3 and with the capsid (By similarity). Interacts with host STING1; this interaction prevents viral DNA-triggered antiviral immune response.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host nucleus</location>
        </subcellularLocation>
        <text evidence="2">Localizes in the host nucleus up to 18 hours postinfection, but at later times localizes to punctate, cytoplasmic structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae cytoplasmic envelopment protein 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081541.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HAC7"/>
    <dbReference type="DNASU" id="3077496"/>
    <dbReference type="GeneID" id="3077496"/>
    <dbReference type="KEGG" id="vg:3077496"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072517">
        <property type="term" value="C:host cell viral assembly compartment"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039503">
        <property type="term" value="P:suppression by virus of host innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04039">
        <property type="entry name" value="HSV_CEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004286">
        <property type="entry name" value="Herpes_UL16/UL94"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03044">
        <property type="entry name" value="Herpes_UL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-0426">Late protein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000417840" description="Cytoplasmic envelopment protein 2">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Nuclear localization signal 1" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Nuclear export signal" evidence="1">
        <location>
            <begin position="55"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Nuclear localization signal 2" evidence="1">
        <location>
            <begin position="90"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04039"/>
        </source>
    </evidence>
    <sequence length="345" mass="38294" checksum="B0046B59FEFC2401" modified="2011-06-28" version="1">MAWRSGLCETDSRTLKQFLQEECMWKLVGKSRKHREYRAVACRSTIFSPEDDGSCILCQLLLFYRDGEWILCLCCNGRYQGHYGVGHVHRRRRRICHLPTLYQLSFGGPLGPASIDFLPSFSQVTSSMTCDGITPDVIYEVCMLVPQDEAKRILVKGHGAMDLTCQKAVTLGGAGAWLLPRPEGYTLFFYILCYDLFTSCGNRCDIPSMTRLMAAATACGQAGCSFCTDHEGHVDPTGNYVGCTPDMGRCLCYVPCGPMTQSLIHNEEPATFFCESDDAKYLCAVGSKTAAQVTLGDGLDYHIGVKDSEGRWLPVKTDVWDLVKVEEPVSRMIVCSCPVLKNLVH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F5HAE6</accession>
    <name>UL15A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL15A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL15A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 UL15A protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081474.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HAE6"/>
    <dbReference type="DNASU" id="3077423"/>
    <dbReference type="GeneID" id="3077423"/>
    <dbReference type="KEGG" id="vg:3077423"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418244" description="Uncharacterized protein UL15A">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="98"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="102" mass="11137" checksum="B1AC4794ABA3061B" modified="2011-06-28" version="1">MKRMIRSHGRKTECQMTGAGERRGSAVGALICGSGTRRGSGANERRDSDVGPIAHSSGTRRGSANETSACTRTDHQKADIGLWFMFLVFGLCSWLAMRYRAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>F5HAM4</accession>
    <name>US31_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein US31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US31</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae US1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081615.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HAM4"/>
    <dbReference type="DNASU" id="3077573"/>
    <dbReference type="GeneID" id="3077573"/>
    <dbReference type="KEGG" id="vg:3077573"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418325" description="Uncharacterized protein US31">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="161" mass="18940" checksum="B6BB1702B3E81D24" modified="2011-06-28" version="1">MSLLEREESWRRVVDYSHNLWCTCGNWQSHVEIQDEEPNCEQPEPAHWLEYVAVQWQARVRDSHDRWCLCNAWRDHALRGRWGTAYSSGSSASSSGFVAESKFTWWKRLRHSTRRWLFRRRRARYTPSNCGESSTSSGQSSGDESNCSLRTHGVYTRGEQH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5HAQ7</accession>
    <name>UL135_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL135</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL135</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2014" name="Cell Host Microbe" volume="16" first="201" last="214">
            <title>HCMV pUL135 remodels the actin cytoskeleton to impair immune recognition of infected cells.</title>
            <authorList>
                <person name="Stanton R.J."/>
                <person name="Prod'homme V."/>
                <person name="Purbhoo M.A."/>
                <person name="Moore M."/>
                <person name="Aicheler R.J."/>
                <person name="Heinzmann M."/>
                <person name="Bailer S.M."/>
                <person name="Haas J."/>
                <person name="Antrobus R."/>
                <person name="Weekes M.P."/>
                <person name="Lehner P.J."/>
                <person name="Vojtesek B."/>
                <person name="Miners K.L."/>
                <person name="Man S."/>
                <person name="Wilkie G.S."/>
                <person name="Davison A.J."/>
                <person name="Wang E.C."/>
                <person name="Tomasec P."/>
                <person name="Wilkinson G.W."/>
            </authorList>
            <dbReference type="PubMed" id="25121749"/>
            <dbReference type="DOI" id="10.1016/j.chom.2014.07.005"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>INTERACTION WITH HOST ABI1; ABI2; NAP1; WASF2 AND TLN1</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Remodels the host actin cytoskeleton in order to impair immune recognition of infected cells. Mechanistically, interacts with members of the host WAVE2 complex and redirects the complex to the plasma membrane. In turn, the efficiency of immune synapse formation is greatly reduced.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with host components of the WAVE2 complex ABI1, NAP1 and WAVE2. Interacts also with host ABI2 and TLN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Host Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HCMV UL135 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081580.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BioGRID" id="1678011">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HAQ7"/>
    <dbReference type="DNASU" id="3077458"/>
    <dbReference type="GeneID" id="3077458"/>
    <dbReference type="KEGG" id="vg:3077458"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1131">Modulation of host NK-cell activity by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416731" description="Protein UL135">
        <location>
            <begin position="23"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="25121749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="308" mass="33281" checksum="B94B2285BBB9752E" modified="2011-06-28" version="1" precursor="true">MVWLWLGVGLLGGTGLASLVLAISLFTQRRGRKRSDETSSRGRLPGAASDKRGACACCYRIPKEDVVEPLDLELGLMRVATHPPTPQVPRCTSLYIGEDGLPIDKPEFPPARFEIPDVSTPGTPTSIGRSPSHCSSSSSLSSSASVDTVLHQPPPSWKPPPPPGRKKRPPTPPVRAPTTRLSSHRPPTPIPAPRKNLSTPPTKKTPPPTKPKPVGWTPPVTPRPFPKTPTPQKPPRNPRLPRTVGLENLSKVGLSCPCPRPRTPTEPTTLPIVSVSELAPPPRWSDIEELLEKAVQSVMKDAESMQMT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>F5HAS7</accession>
    <name>UL92_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL92</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL92</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081539.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HAS7"/>
    <dbReference type="DNASU" id="3077564"/>
    <dbReference type="GeneID" id="3077564"/>
    <dbReference type="KEGG" id="vg:3077564"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004289">
        <property type="entry name" value="Herpes_UL92"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03048">
        <property type="entry name" value="Herpes_UL92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418290" description="Uncharacterized protein UL92">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <sequence length="201" mass="22511" checksum="BA8CB964A75DFF53" modified="2011-06-28" version="1">MCDASGACDMRHVQNAFTEEIQLHSLYACTRCFRTHLCDLGSGCALVSTLEGSVCVKTGLVYEALYPVARSHLLEPIEEAALDDVNIISAVLSGVYSYLMTHAGRYADVIQEVVERDRLKKQVEDSIYFTFNKVFRSMHNVNRISVPVISQLFIQLIIGIYSKQTKYDACVIKVSRKKREDALLKQMRSEYGNAPVFGSGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F5HAY6</accession>
    <name>VICA_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Viral inhibitor of caspase-8-induced apoptosis</fullName>
            <shortName>vICA</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL36</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7829" last="7834">
            <title>A cytomegalovirus-encoded inhibitor of apoptosis that suppresses caspase-8 activation.</title>
            <authorList>
                <person name="Skaletskaya A."/>
                <person name="Bartle L.M."/>
                <person name="Chittenden T."/>
                <person name="McCormick A.L."/>
                <person name="Mocarski E.S."/>
                <person name="Goldmacher V.S."/>
            </authorList>
            <dbReference type="PubMed" id="11427719"/>
            <dbReference type="DOI" id="10.1073/pnas.141108798"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HOST CASP8</scope>
        <scope>MUTAGENESIS OF CYS-131</scope>
        <source>
            <strain>Towne</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the inhibition of apoptosis by interacting with the pro-domain of pro-caspase-8/CASP8 and thus preventing its activation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host pro-caspase-8/CASP8; this interaction inhibits CASP8 activation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae US22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081495.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HAY6"/>
    <dbReference type="GeneID" id="3077442"/>
    <dbReference type="KEGG" id="vg:3077442"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1085">Inhibition of host caspases by virus</keyword>
    <keyword id="KW-1119">Modulation of host cell apoptosis by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418299" description="Viral inhibitor of caspase-8-induced apoptosis">
        <location>
            <begin position="1"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Complete loss of interaction with Pro-caspase 8." evidence="1">
        <original>C</original>
        <variation>R</variation>
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="11427719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="476" mass="54924" checksum="BEF2E05AA58785FA" modified="2011-06-28" version="1">MDDLRDTLMAYGCIAIRAGDFNGLNDFLEQECGTRLHVAWPERCFIQLRSRSALGPFVGKMGTVCSQGAYVCCQEYLHPFGFVEGPGFMRYQLIVLIGQRGGIYCYDDLRDCIYELAPTMKDFLRHGFRHCDHFHTMRDYQRPMVQYDDYWNAVMLYRGDVESLSAEVTKRGYASYSIDDPFDECPDTHFAFWTHNTEVMKFKETSFSVVRAGGSIQTMELMIRTVPRITCYHQLLGALGHEVPERKEFLVRQYVLVDTFGVVYGYDPAMDAVYRLAEDVVMFTCVMGKKGHRNHRFSGRREAIVRLEKTPTCQHPKKTPDPMIMFDEDDDDELSLPRNVMTHEEAESRLYDAITENLMHCVKLVTTDSPLATHLWPQELQALCDSPALSLCTDDVEGVRQKLRARTGSLHHFELSYRFHDEDPETYMGFLWDIPSCDRCVRRRRFKVCDVGRRHIIPGAANGMPPLTPPHAYMNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5HB40</accession>
    <name>UL84_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL84</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL84</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an essential role in viral DNA replication. May participate in the DNA replication initiation by interacting with the origin of lytic replication, oriLyt and subsequently recruiting other viral/cellular factors. Additionally, interacts with and shuttles IRS1 viral mRNA from the host nucleus to the cytoplasm.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with the DNA polymerase accessory subunit UL44. Interacts with HCMV major transcription-activating enzyme IE2. Interacts with host HNRNPK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Shuttles between host nucleus and cytoplasm. In the nucleus, colocalizes with UL44 and IE2 in the viral DNA replication compartments (By similarity).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 UL84 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081532.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HB40"/>
    <dbReference type="GeneID" id="3077525"/>
    <dbReference type="KEGG" id="vg:3077525"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010436">
        <property type="entry name" value="Herpes_UL84"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06284">
        <property type="entry name" value="Cytomega_UL84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417848" description="Protein UL84">
        <location>
            <begin position="1"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear export signal 1 (NES 1)" evidence="1">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear export signal 2 (NES 2)" evidence="1">
        <location>
            <begin position="360"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="587" mass="65478" checksum="C1DDC681EDA0D238" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5HB41</accession>
    <name>US30_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein US30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US30</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081614.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HB41"/>
    <dbReference type="DNASU" id="3077526"/>
    <dbReference type="GeneID" id="3077526"/>
    <dbReference type="KEGG" id="vg:3077526"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035121">
        <property type="entry name" value="US30"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17624">
        <property type="entry name" value="US30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000417854" description="Uncharacterized protein US30">
        <location>
            <begin position="1"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="349" mass="39097" checksum="C1ED4DE308C9A6FA" modified="2011-06-28" version="1">MGNPRSPLDRRLRFRNVPFLPPRIAASSTTTARSLKAKTMEMRFTIAWMWFPSVLLILGLLTPPSNGCTVDVGRNVSIREQCRLRNGATFSKGDIEGNFSGPVVVELDYEDIDITGERQRLRFHLSGLGCPTKENIRKDNESDVNGGIRWALYIQTGDAKYGIRNQHLSIRLMYPGEKNTQQLLGSDFSCERHRRPSTPLGKNAEVPPATRTSSTYGVLSAFVVWIGSGLNIIWWTGIVLLAADALGLGERWLRLALSHRDKHHASRTAALQCQRDMLLRQRRRARRLHAVSEGKLQEEKKRQSALVWNVEARPFPSTHQLIVLPPPVASAPPAVPSQPPEYSSVFPPV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F5HB52</accession>
    <name>US08_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane glycoprotein US8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US8</name>
        <name type="ORF">HHV5wtgp139</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Host endoplasmic reticulum membrane</location>
            <topology>Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytomegalovirus US6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081593.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HB52"/>
    <dbReference type="DNASU" id="3077426"/>
    <dbReference type="GeneID" id="3077426"/>
    <dbReference type="KEGG" id="vg:3077426"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044178">
        <property type="term" value="C:host cell Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044386">
        <property type="term" value="C:integral to host endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012536">
        <property type="entry name" value="CMV_US"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08001">
        <property type="entry name" value="CMV_US"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0393">Immunoglobulin domain</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416723" description="Membrane glycoprotein US8">
        <location>
            <begin position="22"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="22"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like H-type">
        <location>
            <begin position="61"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="69"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="227" mass="26658" checksum="C2B4C229981C68AB" modified="2011-06-28" version="1" precursor="true">MRRWLRLLVGLGCCWVTLAHAGNPYEDDDYYYYREDEPRQHGEPNYVAPPARQFRFPPLNNVSSYQASCVVKDGVLDAVWRVQGTFYPEKGIVARVGWSGRRGRKWGRLHAPECLVETTEAVFRLRQWVPTDLDHLTLHLVPCTKCKPMWCQPRYHIRYFSYGNSVDNLRRLHYEYRHLELGVVIAIQMAMVLLLGYVLARTVYRVSSAYYLRWHACVPQKCEKSLC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F5HBC6</accession>
    <name>PP71_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein pp71</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL82</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Stimulates viral immediate-early (IE) transcription. Plays a role in the inhibition of the host innate repsonse by targeting STING1 and thus the cGAS-STING pathway. Counteracts also host DAXX-mediated repression of viral transcription. Displaces a DAXX-binding protein, ATRX, from nuclear domain 10 sites (ND10) shortly after infection. Increases the basal level of SUMOylated DAXX in infected cells. Stimulates quiescent cells to re-enter the cell cycle, proceed through G1 and enter the S phase. Interacts with hypophosphorylated forms of RB1 and induces their degradation by the proteasome without involving ubiquitin conjugation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with the host protein DAXX; this interaction takes place at ND10 and induces the reversal of DAXX-mediated repression of viral transcription. Interacts with UL35. Interacts with host TMEM173/STING1; this interaction inhibits the cGAS/STING pathway. Interacts with host RB1; this interaction mediates RB1 proteasomal degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host endoplasmic reticulum</location>
        </subcellularLocation>
        <text evidence="1">Present in the nucleus shortly after infection as well as during the late phase of viral morphogenesis. Detected at nuclear domain 10 (ND10).</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">S-nitrosylation limits ability to undermine the cGAS/STING antiviral pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae pp71 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081530.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BioGRID" id="1678083">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HBC6"/>
    <dbReference type="GeneID" id="3077530"/>
    <dbReference type="KEGG" id="vg:3077530"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044165">
        <property type="term" value="C:host cell endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039695">
        <property type="term" value="P:DNA-templated viral transcription"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039645">
        <property type="term" value="P:modulation by virus of host G1/S transition checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008649">
        <property type="entry name" value="Herpes_UL82/UL83"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05784">
        <property type="entry name" value="Herpes_UL82_83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-1078">G1/S host cell cycle checkpoint dysregulation by virus</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1121">Modulation of host cell cycle by virus</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0702">S-nitrosylation</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000418269" description="Protein pp71">
        <location>
            <begin position="1"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="530"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-nitrosocysteine; by host" evidence="1">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine" evidence="1">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P06726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="559" mass="62008" checksum="C79469D33130265B" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F5HBR4</accession>
    <name>PORTL_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Portal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL104</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Forms a portal in the viral capsid through which viral DNA is translocated during DNA packaging. Assembles as a dodecamer at a single fivefold axe of the T=16 icosahedric capsid. Binds to the molecular motor that translocates the viral DNA, termed terminase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homododecamerizes. Interacts with terminase subunits TRM1 and TRM3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae portal protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081550.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HBR4"/>
    <dbReference type="PRIDE" id="F5HBR4"/>
    <dbReference type="GeneID" id="3077547"/>
    <dbReference type="KEGG" id="vg:3077547"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019028">
        <property type="term" value="C:viral capsid"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019076">
        <property type="term" value="P:viral release from host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04012">
        <property type="entry name" value="HSV_PORTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002660">
        <property type="entry name" value="Herpes_Portal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01763">
        <property type="entry name" value="Herpes_UL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0231">Viral genome packaging</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000418268" description="Portal protein">
        <location>
            <begin position="1"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="633"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="661"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="697" mass="78535" checksum="D166B32C875E321A" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F5HC14</accession>
    <name>UL119_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Viral Fc-gamma receptor-like protein UL119</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>gp68</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">UL119/UL118</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Serves as a receptor for the Fc part of human IgG. May thus be involved in interfering with host Ig-mediated immune responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Virion membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081558.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HC14"/>
    <dbReference type="GeneID" id="3077514"/>
    <dbReference type="KEGG" id="vg:3077514"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055036">
        <property type="term" value="C:virion membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075528">
        <property type="term" value="P:modulation by virus of host immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0393">Immunoglobulin domain</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1124">Modulation of host immunity by viral IgG Fc receptor-like protein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000417836" description="Viral Fc-gamma receptor-like protein UL119">
        <location>
            <begin position="24"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="2">
        <location>
            <begin position="24"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="2">
        <location>
            <begin position="315"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like V-type">
        <location>
            <begin position="91"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="345" mass="38711" checksum="D776336BD4549325" modified="2011-06-28" version="1" precursor="true">MCSVLAIALVVALLGDMHPGVKSSTTSAVTSPSNTTVTSTTSISTSNNVSSAVTTTVQTSTSSASTSVIATTQKEGHLYTVNCEASYSYDQVSLNATCKVILLNNTKNPDILSVTCYARTDCKGPFTQVGYLSAFPSNDKGKLHLSYNATAQELLISGLRPQETTEYTCSFFSWGRHHNATWDLFTYPIYAVYGTRLNATTMRVRVLLQEHEHCLLNGSSLYHPNSTVHLHQGDQLIPPWNISNVTYNGQRLREFVFYLNGTYTVVRLHVQIAGRSFTTTYVFIKSDPLFEDRLLAYGVLAFLVFMVIILLYVTYMLARRRDWSYKRLEEPVEEKKHPVPYFKQW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F5HC33</accession>
    <name>US09_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane glycoprotein US9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US9</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Influences cell-to-cell spread of virus in polarized cells. Promotes dissemination of virus across cell-cell junctions of polarized epithelial cells, maybe through the association with the cytoskeletal matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Host endoplasmic reticulum membrane</location>
            <topology>Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
            <location evidence="1">Host cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">In polarized cells, colocalizes with F-actin in the cortical cytoskeleton which underlies lateral membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytomegalovirus US6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081594.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HC33"/>
    <dbReference type="DNASU" id="3077455"/>
    <dbReference type="GeneID" id="3077455"/>
    <dbReference type="KEGG" id="vg:3077455"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044178">
        <property type="term" value="C:host cell Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044163">
        <property type="term" value="C:host cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044386">
        <property type="term" value="C:integral to host endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012536">
        <property type="entry name" value="CMV_US"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08001">
        <property type="entry name" value="CMV_US"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1037">Host cytoskeleton</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0393">Immunoglobulin domain</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416724" description="Membrane glycoprotein US9">
        <location>
            <begin position="28"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="28"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="214"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like H-type">
        <location>
            <begin position="72"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="81"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="247" mass="28053" checksum="D8B0EBA23A8B7067" modified="2011-06-28" version="1" precursor="true">MILWSPSTCSFFWHWCLIAVSVLSSRSKESLRLSWSSDESSASSSSRICPLSNSKSVRLPQYPRGFGDVSGYRVSSSVSECYVQHGVLVAAWLVRGNFSDTAPRAYGTWGNERSATHFKVGAPQLENDGALRYETELPQVDARLSYVMLTVYPCSACNRSVLHCRPASRLPWLPLRVTPSDLERLFAERRYLTFLYVVLVQFVKHVALFSFGVQVACCVYLRWIRPWVRGRHRATGRTSREEEAKDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-02-23" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F5HC71</accession>
    <name>IL10H_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Viral interleukin-10 homolog</fullName>
            <shortName>cmvIL-10</shortName>
            <shortName>vIL-10</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL111A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2003" name="J. Gen. Virol." volume="84" first="1117" last="1122">
            <title>Two novel spliced genes in human cytomegalovirus.</title>
            <authorList>
                <person name="Akter P."/>
                <person name="Cunningham C."/>
                <person name="McSharry B.P."/>
                <person name="Dolan A."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="12692276"/>
            <dbReference type="DOI" id="10.1099/vir.0.18952-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Functional viral IL-10 homolog. Can bind to the human IL-10 receptor and compete with human IL-10 for binding sites. Requires both subunits of the human IL-10 receptor complex to induce signal transduction events and biological activities. IL-10 signaling pathway has several immunosuppressive activities that are exploited by the virus. Inhibits TLR-induced type I interferon production in host plasmacytoid dendritic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IL-10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081552.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HC71"/>
    <dbReference type="PRIDE" id="F5HC71"/>
    <dbReference type="GeneID" id="3077506"/>
    <dbReference type="KEGG" id="vg:3077506"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020443">
        <property type="entry name" value="IL-10/19/20/22/24/26_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012352">
        <property type="entry name" value="IL-10_add_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020423">
        <property type="entry name" value="IL-10_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00726">
        <property type="entry name" value="IL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00188">
        <property type="entry name" value="IL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="SSF47266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00520">
        <property type="entry name" value="INTERLEUKIN_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0202">Cytokine</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-1125">Evasion of host immunity by viral interleukin-like protein</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-1114">Inhibition of host interferon signaling pathway by virus</keyword>
    <keyword id="KW-1118">Modulation of host dendritic cell activity by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416438" description="Viral interleukin-10 homolog">
        <location>
            <begin position="26"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="38"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="82"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="176" mass="20108" checksum="DAC25EBFDFA9A872" modified="2011-06-28" version="1" precursor="true">MLSVMVSSSLVLIVFFLGASEEAKPATTTTIKNTKPQCRPEDYATRLQDLRVTFHRVKPTLQREDDYSVWLDGTVVKGCWGCSVMDWLLRRYLEIVFPAGDHVYPGLKTELHSMRSTLESIYKDMRQCPLLGCGDKSVISRLSQEAERKSDNGTRKGLSELDTLFSRLEEYLHSRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F5HC97</accession>
    <name>VPAP_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>DNA polymerase processivity factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Polymerase accessory protein</fullName>
            <shortName>PAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Protein ICP36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">UL44</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the DNA polymerase that plays an essential role in viral DNA replication and acts by increasing the processivity of polymerization. Forms dimers that binds to double-stranded DNA and UL54 specifically to stimulates long chain DNA synthesis efficiently. Plays an important role in maintaining the structure of viral replication compartments by interacting with host nucleolin/NUC. In addition, suppresses innate immune responses through effects on host IRF3 and NF-kappa-B. Mechanistically, interfere with the binding of IRF3 and the p65 NF-kappa-B subunit to the promoters of antiviral genes, thereby inhibiting the expression of these genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms homodimers. Interacts with host SMARCB1. Interacts with host NCL/nucleolin; this interaction is important for the organization of proteins within viral replication compartments. Interacts with UL112/UL113; this interaction is necessary for efficient viral DNA replication. Interacts with UL84. Interacts with the uracil DNA glycosylase UL114. Interacts with the DNA polymerase catalytic subunit UL54. Interacts with host IRF3. Interacts with host RELA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The C-terminal region is required for viral DNA synthesis.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated by UL97 on serine residues, phosphorylation seems important for UL44 nuclear entry but does not directly affect its role in replication.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Sumoylated. Sumoylation on Lys-410 increases viral DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae polymerase accessory protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081502.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HC97"/>
    <dbReference type="BioGRID" id="1678013">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HC97"/>
    <dbReference type="DNASU" id="3077460"/>
    <dbReference type="GeneID" id="3077460"/>
    <dbReference type="KEGG" id="vg:3077460"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039644">
        <property type="term" value="P:suppression by virus of host NF-kappaB cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039548">
        <property type="term" value="P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039693">
        <property type="term" value="P:viral DNA genome replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004997">
        <property type="entry name" value="Herpes_PAP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03325">
        <property type="entry name" value="Herpes_PAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-1092">Inhibition of host IRF3 by virus</keyword>
    <keyword id="KW-1100">Inhibition of host NF-kappa-B by virus</keyword>
    <keyword id="KW-1113">Inhibition of host RLR pathway by virus</keyword>
    <keyword id="KW-1017">Isopeptide bond</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0832">Ubl conjugation</keyword>
    <keyword id="KW-1194">Viral DNA replication</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000418258" description="DNA polymerase processivity factor">
        <location>
            <begin position="1"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="363"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="433" mass="46263" checksum="DC7669CA7121B2C0" modified="2011-06-28" version="1">MDRKTRLSEPPTLALRLKPYKTAIQQLRSVIRALKENTTVTFLPTPSLILQTVRSHCVSKITFNSSCLYITDKSFQPKTINNSTPLLGNFMYLTSSKDLTKFYVQDISDLSAKISMCAPDFNMEFSSACVHGQDIVRESENSAVHVDLDFGVVADLLKWIGPHTRVKRNVKKAPCPTGTVQILVHAGPPAIKFILTNGSELEFTANNRVSFHGVKNMRINVQLKNFYQTLLNCAVTKLPCTLRIVTEHDTLLYVASRNGLFAVENFLTEEPFQRGDPFDKNYVGNSGKSRGGGGGSGSLSSLANAGGLHDDGPGLDNDIMNEPMGLGGLGGGGGGGGKKHDRGGGGGSGTRKMSSGGGGGDHDHGLSSKEKYEQHKITSYLTSKGGSGGGGGGGGGGLDRNSGNYFNDAKEESDSEDSVTFEFVPNTKKQKCG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F5HCH8</accession>
    <name>GL_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Envelope glycoprotein L</fullName>
            <shortName evidence="1">gL</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gL</name>
        <name type="ORF">UL115</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="J. Virol." volume="82" first="60" last="70">
            <title>Characterization of the human cytomegalovirus gH/gL/UL128-131 complex that mediates entry into epithelial and endothelial cells.</title>
            <authorList>
                <person name="Ryckman B.J."/>
                <person name="Rainish B.L."/>
                <person name="Chase M.C."/>
                <person name="Borton J.A."/>
                <person name="Nelson J.A."/>
                <person name="Jarvis M.A."/>
                <person name="Johnson D.C."/>
            </authorList>
            <dbReference type="PubMed" id="17942555"/>
            <dbReference type="DOI" id="10.1128/JVI.01910-07"/>
        </citation>
        <scope>INTERACTION WITH UL128; UL130; UL131 AND GH</scope>
        <source>
            <strain>TR</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2017" name="PLoS Pathog." volume="13" first="E1006281" last="E1006281">
            <title>Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-alpha as a key for entry.</title>
            <authorList>
                <person name="Wu Y."/>
                <person name="Prager A."/>
                <person name="Boos S."/>
                <person name="Resch M."/>
                <person name="Brizic I."/>
                <person name="Mach M."/>
                <person name="Wildner S."/>
                <person name="Scrivano L."/>
                <person name="Adler B."/>
            </authorList>
            <dbReference type="PubMed" id="28403202"/>
            <dbReference type="DOI" id="10.1371/journal.ppat.1006281"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH GH AND PDGFRA</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2018" name="Cell" volume="0" first="0" last="0">
            <title>An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.</title>
            <authorList>
                <person name="Martinez-Martin N."/>
                <person name="Marcandalli J."/>
                <person name="Huang C.S."/>
                <person name="Arthur C.P."/>
                <person name="Perotti M."/>
                <person name="Foglierini M."/>
                <person name="Ho H."/>
                <person name="Dosey A.M."/>
                <person name="Shriver S."/>
                <person name="Payandeh J."/>
                <person name="Leitner A."/>
                <person name="Lanzavecchia A."/>
                <person name="Perez L."/>
                <person name="Ciferri C."/>
            </authorList>
            <dbReference type="PubMed" id="30057110"/>
            <dbReference type="DOI" id="10.1016/j.cell.2018.06.028"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HOST NRP2</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3 4">The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Acts as a functional inhibitor of gH and maintains gH in an inhibited form. Upon binding to host integrins, gL dissociates from gH leading to activation of the viral fusion glycoproteins gB and gH (By similarity). In human cytomegalovirus, forms two distincts complexes to mediate viral entry, a trimer and a pentamer at the surface of the virion envelope. The gH-gL-gO trimer is required for infection in fibroblasts by interacting with host PDGFRA (PubMed:28403202). The gH-gL-UL128-UL130-UL131A pentamer is essential for viral entry in epithelial, endothelial and myeloid cells via interaction with host NRP2 (PubMed:30057110).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3 4">Interacts with glycoprotein H (gH); this interaction is necessary for the correct processing and cell surface expression of gH (By similarity). Forms the envelope pentamer complex (PC) composed of gH, gL, UL128, UL130, and UL131A (PubMed:17942555). The pentamer interacts with host NRP2 (PubMed:30057110). Forms the envelope trimer complex composed of gH, gL, and gO. The trimer interacts with host PDGFRA (PubMed:28403202).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host Golgi apparatus</location>
            <location evidence="1">Host trans-Golgi network</location>
        </subcellularLocation>
        <text evidence="1">gL associates with the extravirion surface through its binding to gH. During virion morphogenesis, this protein probably accumulates in the host trans-Golgi where secondary envelopment occurs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae glycoprotein L family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081555.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PDB" id="7LBE">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="B=1-278"/>
    </dbReference>
    <dbReference type="PDB" id="7LBF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="B=1-278"/>
    </dbReference>
    <dbReference type="PDB" id="7LBG">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="B=1-278"/>
    </dbReference>
    <dbReference type="PDBsum" id="7LBE"/>
    <dbReference type="PDBsum" id="7LBF"/>
    <dbReference type="PDBsum" id="7LBG"/>
    <dbReference type="SMR" id="F5HCH8"/>
    <dbReference type="TCDB" id="1.G.22.1.1">
        <property type="family name" value="the cytomegalovirus (human herpesvirus 5) glycoprotein go (go) family"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HCH8"/>
    <dbReference type="DNASU" id="3077416"/>
    <dbReference type="GeneID" id="3077416"/>
    <dbReference type="KEGG" id="vg:3077416"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044177">
        <property type="term" value="C:host cell Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019031">
        <property type="term" value="C:viral envelope"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055036">
        <property type="term" value="C:virion membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098670">
        <property type="term" value="P:entry receptor-mediated virion attachment to host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019064">
        <property type="term" value="P:fusion of virus membrane with host plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046718">
        <property type="term" value="P:viral entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04036">
        <property type="entry name" value="HSV_GL_betahv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002689">
        <property type="entry name" value="Cytomegalo_gL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01801">
        <property type="entry name" value="Cytomega_gL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1169">Fusion of virus membrane with host cell membrane</keyword>
    <keyword id="KW-1168">Fusion of virus membrane with host membrane</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-1161">Viral attachment to host cell</keyword>
    <keyword id="KW-1234">Viral attachment to host entry receptor</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-1162">Viral penetration into host cytoplasm</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416445" description="Envelope glycoprotein L" evidence="1">
        <location>
            <begin position="31"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="47"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="84"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="94"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="98"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="141"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="151"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="158"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="169"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="173"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="176"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="198"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="216"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="255"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="17942555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="28403202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="30057110"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="7LBE"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="7LBF"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="7LBG"/>
        </source>
    </evidence>
    <sequence length="278" mass="30815" checksum="E2D256850D42F74D" modified="2011-06-28" version="1" precursor="true">MCRRPDCGFSFSPGPVILLWCCLLLPIVSSAAVSVAPTAAEKVPAECPELTRRCLLGEVFEGDKYESWLRPLVNVTGRDGPLSQLIRYRPVTPEAANSVLLDEAFLDTLALLYNNPDQLRALLTLLSSDTAPRWMTVMRGYSECGDGSPAVYTCVDDLCRGYDLTRLSYGRSIFTEHVLGFELVPPSLFNVVVAIRNEATRTNRAVRLPVSTAAAPEGITLFYGLYNAVKEFCLRHQLDPPLLRHLDKYYAGLPPELKQTRVNLPAHSRYGPQAVDAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F5HCM1</accession>
    <name>VIE1_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Immediate early protein IE1</fullName>
            <shortName>IE1</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>55 kDa immediate-early protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">IE1p72</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">IE72</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">UL123</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Plays an important role in transactivating viral early genes as well as activating its own promoter, probably by altering the viral chromatin structure (By similarity). Expression of IE1 and IE2 proteins is critical for the establishment of lytic infection and reactivation from viral latency (By similarity). Disrupts PML-associated ND10 nuclear bodies by interfering with host PML and SP100 sumoylation thereby altering the regulation of type I and type II interferon-induced gene expression (By similarity). Promotes efficient viral growth by interacting with and directing host SP100 to degradation, leading to enhanced acetylation level of histones (By similarity). In addition, functions in counteracting the host innate antiviral response (By similarity). Inhibits the type I interferon pathway by directly interacting with and sequestrating host STAT2 (By similarity). Also targets type II interferon pathway by repressing IL6- and STAT3 target genes (By similarity). Repression of STAT3 genes is due to STAT3 nuclear accumulation and disruption of IL6-induced STAT3 phosphorylation by IE1 (By similarity). This repression is followed by phosphorylation and activation of STAT1 (By similarity). Inhibits host ISG transcription by sequestering host ISGF3 in a PML- and STAT2- binding dependent manner (By similarity). Alters host cell cycle progression, probably through its interaction with host E2F1 or RB1 that overcomes the RB1-mediated repression of E2F-responsive promoters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with human p53/TP53; this interaction inhibits p53/TP53-dependent transactivation activity. Interacts with host STAT1. Interacts with host STAT2; this interaction promotes viral growth and counteracts the antiviral interferon response. May also interact with the host STAT1-STAT2 heterodimer. Interacts with host STAT3; this interaction leads to STAT3 nuclear accumulation and disruption of IL6-induced STAT3 phosphorylation. Interacts with host PML; this interaction probably inhibits PML regulation of type I and type II interferon-induced gene expression. Interacts with host DAXX. Interacts with host SP100. Interacts with host E2F1. Interacts with host RB1. Interacts with host HDAC1; this interaction inhibits histone deacetylation and promotes viral transcription. Interacts with host HDAC2; this interaction inhibits histone deacetylation and promotes viral transcription. Interacts with host HDAC3; this interaction inhibits histone deacetylation and promotes viral transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host nucleus</location>
        </subcellularLocation>
        <text evidence="2">Colocalizes with host PML-associated nuclear bodies very early post infection.</text>
    </comment>
    <comment type="domain">
        <text evidence="2">The N-terminal region is required for nuclear targeting. The C-terminal 16-amino acid is termed the chromosome-tethering domain (CTD) and is required for the association of IE1, host PML and host STAT2 with the mitotic chromosomes. Targets host nucleosomes by directly binding to the acidic pocket of core histones.</text>
    </comment>
    <comment type="PTM">
        <text evidence="2 3">Sumoylated by host PML/nuclear domain 10 (By similarity). Sumoylation abolishes the interaction with host STAT2 and thus the IE1-mediated repression of interferon-stimulated genes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HHV-5 IE1 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081562.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HCM1"/>
    <dbReference type="BioGRID" id="1678066">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HCM1"/>
    <dbReference type="GeneID" id="3077513"/>
    <dbReference type="KEGG" id="vg:3077513"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039695">
        <property type="term" value="P:DNA-templated viral transcription"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039645">
        <property type="term" value="P:modulation by virus of host G1/S transition checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039502">
        <property type="term" value="P:suppression by virus of host type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010855">
        <property type="entry name" value="Cytomega_IE1/IE2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07340">
        <property type="entry name" value="Herpes_IE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-1078">G1/S host cell cycle checkpoint dysregulation by virus</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-1114">Inhibition of host interferon signaling pathway by virus</keyword>
    <keyword id="KW-1017">Isopeptide bond</keyword>
    <keyword id="KW-1121">Modulation of host cell cycle by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0832">Ubl conjugation</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="chain" id="PRO_0000416446" description="Immediate early protein IE1">
        <location>
            <begin position="1"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Nuclear localization signal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with host PML, interference with PML sumoylation and disruption of PML-associated nuclear bodies" evidence="2">
        <location>
            <begin position="132"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with host STAT2" evidence="2">
        <location>
            <begin position="373"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with host STAT3" evidence="2">
        <location>
            <begin position="410"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Modulation of STAT3/STAT1 signaling" evidence="2">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="421"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Acidic" evidence="2">
        <location>
            <begin position="421"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with host SUMO1" evidence="2">
        <location>
            <begin position="449"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Chromosome-tethering domain (CTD), binding to histones" evidence="2">
        <location>
            <begin position="475"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="425"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)" evidence="1">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P03169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="P13202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="491" mass="55103" checksum="E5BE99891AC4A4C3" modified="2011-06-28" version="1">MESSAKRKMDPDNPDEGPSSKVPRPETPVTKATTFLQTMLRKEVNSQLSLGDPLFPELAEESLKTFEQVTEDCNENPEKDVLTELVKQIKVRVDMVRHRIKEHMLKKYTQTEEKFTGAFNMMGGCLQNALDILDKVHEPFEDMKCIGLTMQSMYENYIVPEDKREMWMACIKELHDVSKGAANKLGGALQAKARAKKDELRRKMMYMCYRNIEFFTKNSAFPKTTNGCSQAMAALQNLPQCSPDEIMSYAQKIFKILDEERDKVLTHIDHIFMDILTTCVETMCNEYKVTSDACMMTMYGGISLLSEFCRVLCCYVLEETSVMLAKRPLITKPEVISVMKRRIEEICMKVFAQYILGADPLRVCSPSVDDLRAIAEESDEEEAIVAYTLATAGASSSDSLVSPPESPVPATIPLSSVIVAENSDQEESEQSDEEQEEGAQEEREDTVSVKSEPVSEIEEVASEEEEDGAEEPTASGGKSTHPMVTRSKADQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F5HCP3</accession>
    <name>UL130_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Envelope glycoprotein UL130</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL130</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="J. Virol." volume="82" first="60" last="70">
            <title>Characterization of the human cytomegalovirus gH/gL/UL128-131 complex that mediates entry into epithelial and endothelial cells.</title>
            <authorList>
                <person name="Ryckman B.J."/>
                <person name="Rainish B.L."/>
                <person name="Chase M.C."/>
                <person name="Borton J.A."/>
                <person name="Nelson J.A."/>
                <person name="Jarvis M.A."/>
                <person name="Johnson D.C."/>
            </authorList>
            <dbReference type="PubMed" id="17942555"/>
            <dbReference type="DOI" id="10.1128/JVI.01910-07"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH GH; GL; UL128 AND UL131A</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2013" name="PLoS Pathog." volume="9" first="E1003463" last="E1003463">
            <title>The HCMV gH/gL/UL128-131 complex triggers the specific cellular activation required for efficient viral internalization into target monocytes.</title>
            <authorList>
                <person name="Nogalski M.T."/>
                <person name="Chan G.C."/>
                <person name="Stevenson E.V."/>
                <person name="Collins-McMillen D.K."/>
                <person name="Yurochko A.D."/>
            </authorList>
            <dbReference type="PubMed" id="23853586"/>
            <dbReference type="DOI" id="10.1371/journal.ppat.1003463"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2013" name="J. Virol." volume="87" first="9680" last="9690">
            <title>Comparative analysis of gO isoforms reveals that strains of human cytomegalovirus differ in the ratio of gH/gL/gO and gH/gL/UL128-131 in the virion envelope.</title>
            <authorList>
                <person name="Zhou M."/>
                <person name="Yu Q."/>
                <person name="Wechsler A."/>
                <person name="Ryckman B.J."/>
            </authorList>
            <dbReference type="PubMed" id="23804643"/>
            <dbReference type="DOI" id="10.1128/JVI.01167-13"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2018" name="Cell" volume="0" first="0" last="0">
            <title>An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.</title>
            <authorList>
                <person name="Martinez-Martin N."/>
                <person name="Marcandalli J."/>
                <person name="Huang C.S."/>
                <person name="Arthur C.P."/>
                <person name="Perotti M."/>
                <person name="Foglierini M."/>
                <person name="Ho H."/>
                <person name="Dosey A.M."/>
                <person name="Shriver S."/>
                <person name="Payandeh J."/>
                <person name="Leitner A."/>
                <person name="Lanzavecchia A."/>
                <person name="Perez L."/>
                <person name="Ciferri C."/>
            </authorList>
            <dbReference type="PubMed" id="30057110"/>
            <dbReference type="DOI" id="10.1016/j.cell.2018.06.028"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HOST NRP2</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2017" name="Sci. Immunol." volume="2" first="0" last="0">
            <title>Structural basis for potent antibody-mediated neutralization of human cytomegalovirus.</title>
            <authorList>
                <person name="Chandramouli S."/>
                <person name="Malito E."/>
                <person name="Nguyen T."/>
                <person name="Luisi K."/>
                <person name="Donnarumma D."/>
                <person name="Xing Y."/>
                <person name="Norais N."/>
                <person name="Yu D."/>
                <person name="Carfi A."/>
            </authorList>
            <dbReference type="PubMed" id="28783665"/>
            <dbReference type="DOI" id="10.1126/sciimmunol.aan1457"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.02 ANGSTROMS) OF 1-214</scope>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH GH; GL; UL128 AND UL130</scope>
    </reference>
    <comment type="function">
        <text evidence="2 4 5 6">Plays a role in viral entry into host cells. Forms a pentameric complex at the surface of the viral envelope together with gH, gL, UL130 and UL131. This complex is required for entry in epithelial, endothelial and myeloid host cells (PubMed:23853586, PubMed:28783665, PubMed:17942555). Mechanistically, engages host receptor(s) including neurophilin 2/NRP2 to mediate infection (PubMed:30057110).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5 6">Forms the envelope pentamer complex (PC) composed of gH, gL, UL128, UL130, and UL131A. The pentamer interacts with host NRP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Virion membrane</location>
        </subcellularLocation>
        <text evidence="3">Found as a pentameric complex at the surface of virion envelope.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the HHV-5 UL130 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081565.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PDB" id="5VOB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.02 A"/>
        <property type="chains" value="D=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="5VOC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.99 A"/>
        <property type="chains" value="D=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="5VOD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="5.90 A"/>
        <property type="chains" value="D=1-214"/>
    </dbReference>
    <dbReference type="PDBsum" id="5VOB"/>
    <dbReference type="PDBsum" id="5VOC"/>
    <dbReference type="PDBsum" id="5VOD"/>
    <dbReference type="SMR" id="F5HCP3"/>
    <dbReference type="PRIDE" id="F5HCP3"/>
    <dbReference type="ABCD" id="F5HCP3">
        <property type="antibodies" value="1 sequenced antibody"/>
    </dbReference>
    <dbReference type="DNASU" id="3077481"/>
    <dbReference type="GeneID" id="3077481"/>
    <dbReference type="KEGG" id="vg:3077481"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021038">
        <property type="entry name" value="Herpes_UL130_cytomegalovirus"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11668">
        <property type="entry name" value="Gp_UL130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-1161">Viral attachment to host cell</keyword>
    <keyword id="KW-1234">Viral attachment to host entry receptor</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418314" description="Envelope glycoprotein UL130">
        <location>
            <begin position="26"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="49"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="68"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="71"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="81"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="87"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="98"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="206"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="17942555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="23804643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="23853586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="28783665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="30057110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="5VOB"/>
        </source>
    </evidence>
    <sequence length="214" mass="24709" checksum="E76147BF59E30CF5" modified="2011-06-28" version="1" precursor="true">MLRLLLRHHFHCLLLCAVWATPCLASPWSTLTANQNPSPPWSKLTYSKPHDAATFYCPFLYPSPPRSPLQFSGFQRVSTGPECRNETLYLLYNREGQTLVERSSTWVKKVIWYLSGRNQTILQRMPRTASKPSDGNVQISVEDAKIFGAHMVPKQTKLLRFVVNDGTRYQMCVMKLESWAHVFRDYSVSFQVRLTFTEANNQTYTFCTHPNLIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F5HCU8</accession>
    <name>TRM3_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tripartite terminase subunit 3</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Terminase large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">TRM3</name>
        <name type="ordered locus">UL89</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the molecular motor that translocates viral genomic DNA in empty capsid during DNA packaging. Forms a tripartite terminase complex together with TRM1 and TRM2 in the host cytoplasm. Once the complex reaches the host nucleus, it interacts with the capsid portal vertex. This portal forms a ring in which genomic DNA is translocated into the capsid. TRM3 carries an RNase H-like nuclease activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with the terminase subunits TRM1 and TRM2. Interacts with portal protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <text evidence="1">Responsible for the nuclear localization of the two others subunits TRM1 and TRM2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae TRM3 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081537.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BMRB" id="F5HCU8"/>
    <dbReference type="SMR" id="F5HCU8"/>
    <dbReference type="DrugBank" id="DB12070">
        <property type="generic name" value="Letermovir"/>
    </dbReference>
    <dbReference type="DrugCentral" id="F5HCU8"/>
    <dbReference type="PRIDE" id="F5HCU8"/>
    <dbReference type="GeneID" id="3077553"/>
    <dbReference type="KEGG" id="vg:3077553"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019076">
        <property type="term" value="P:viral release from host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04013">
        <property type="entry name" value="HSV_TRM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003498">
        <property type="entry name" value="DNA_pack_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038435">
        <property type="entry name" value="DNA_pack_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003499">
        <property type="entry name" value="DNA_pack_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033663">
        <property type="entry name" value="HSV_TRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02499">
        <property type="entry name" value="DNA_pack_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02500">
        <property type="entry name" value="DNA_pack_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0231">Viral genome packaging</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <feature type="chain" id="PRO_0000417832" description="Tripartite terminase subunit 3">
        <location>
            <begin position="1"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Walker A motif" evidence="1">
        <location>
            <begin position="212"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Walker B motif" evidence="1">
        <location>
            <begin position="305"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="active site" description="For ATPase activity" evidence="1">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="active site" description="For nuclease activity" evidence="1">
        <location>
            <position position="463"/>
        </location>
    </feature>
    <feature type="active site" description="For nuclease activity" evidence="1">
        <location>
            <position position="534"/>
        </location>
    </feature>
    <feature type="active site" description="For nuclease activity" evidence="1">
        <location>
            <position position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04013"/>
        </source>
    </evidence>
    <sequence length="674" mass="77034" checksum="EAE1087E3A907CAA" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5HD03</accession>
    <name>US32_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein US32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US32</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae US1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081616.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HD03"/>
    <dbReference type="DNASU" id="3077487"/>
    <dbReference type="GeneID" id="3077487"/>
    <dbReference type="KEGG" id="vg:3077487"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000416729" description="Protein US32">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="183" mass="22085" checksum="EF59C0BBC6B7B4F1" modified="2011-06-28" version="1">MAMYTSESERDWRRVIHDSHGLWCDCGDWREHLYCVYDSHFQRRPTTRAERRAANWRRQMRRLHRLWCFCQDWKCHALYAEWDGKESDDESSASSSGEAPEQQVPAWKTVRAFSRAYHHRINRGLRGTPPPRNLPGYEHASEGWRFCNRRERREDDLRTRAEPDRVVFQLGGVPPRRHRETYV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-02-23" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5HD27</accession>
    <name>UL121_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane protein UL121</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL121</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HHV-5 UL121 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081560.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HD27"/>
    <dbReference type="GeneID" id="3077529"/>
    <dbReference type="KEGG" id="vg:3077529"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022545">
        <property type="entry name" value="HHV_UL121"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10838">
        <property type="entry name" value="DUF2677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416447" description="Membrane protein UL121">
        <location>
            <begin position="28"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="180" mass="20253" checksum="F150E34237068A88" modified="2011-06-28" version="1" precursor="true">MWGCGWSRILVLLLLMCMALMARGTYGAYICSPNPGRLRISCALSVLDQRLWWEIQYSSGRLTRVLVFHDEGEEGDDVHLTDTHHCTSCTHPYVISLVTPLTINATLRLLIRDGMYGRGEKELCIAHLPTLRDIRTCRVDADLGLLYAVCLILSFSIVTAALWKVDYDRSVAVVSKSYKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F5HDD3</accession>
    <name>US07_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane glycoprotein US7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US7</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytomegalovirus US6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081592.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HDD3"/>
    <dbReference type="DNASU" id="3077535"/>
    <dbReference type="GeneID" id="3077535"/>
    <dbReference type="KEGG" id="vg:3077535"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044386">
        <property type="term" value="C:integral to host endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012536">
        <property type="entry name" value="CMV_US"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08001">
        <property type="entry name" value="CMV_US"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0393">Immunoglobulin domain</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000417853" description="Membrane glycoprotein US7">
        <location>
            <begin position="18"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="18"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="192"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like H-type">
        <location>
            <begin position="54"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="63"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="225" mass="26272" checksum="F82BD9D55EBEEFFD" modified="2011-06-28" version="1" precursor="true">MRIQLLLVSTLVASIVATRVEDMATFRTEKQWQQDLQYRREFVKRQLAPKPKSNIVVSHTVSCVIDGGNMTSVWRFEGQFNPHIASEVILHDTSGLYNVPHEVQNDGQVLTVTVKRSAPADIAKVLISLKPVQLSSGQYECRPQLQLPWVPRPSSFMYDSYRLWYEKRWLTIILYVFMWTYLVTLLQYCIVRFIGTRLFYFLQRNITIRFTGKPTYNLLTYPVKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-02-23" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F5HDK1</accession>
    <name>US27_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Envelope glycoprotein US27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US27</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2003" name="J. Gen. Virol." volume="84" first="657" last="663">
            <title>Homology between the human cytomegalovirus RL11 gene family and human adenovirus E3 genes.</title>
            <authorList>
                <person name="Davison A.J."/>
                <person name="Akter P."/>
                <person name="Cunningham C."/>
                <person name="Dolan A."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Wilkinson G.W."/>
            </authorList>
            <dbReference type="PubMed" id="12604818"/>
            <dbReference type="DOI" id="10.1099/vir.0.18856-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="J. Gen. Virol." volume="84" first="1117" last="1122">
            <title>Two novel spliced genes in human cytomegalovirus.</title>
            <authorList>
                <person name="Akter P."/>
                <person name="Cunningham C."/>
                <person name="McSharry B.P."/>
                <person name="Dolan A."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="12692276"/>
            <dbReference type="DOI" id="10.1099/vir.0.18952-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="J. Gen. Virol." volume="84" first="2511" last="2516">
            <title>The most abundantly transcribed human cytomegalovirus gene (beta 2.7) is non-essential for growth in vitro.</title>
            <authorList>
                <person name="McSharry B.P."/>
                <person name="Tomasec P."/>
                <person name="Neale M.L."/>
                <person name="Wilkinson G.W."/>
            </authorList>
            <dbReference type="PubMed" id="12917473"/>
            <dbReference type="DOI" id="10.1099/vir.0.19298-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2011" name="J. Virol." volume="85" first="3700" last="3707">
            <title>Human cytomegalovirus pUS27 G protein-coupled receptor homologue is required for efficient spread by the extracellular route but not for direct cell-to-cell spread.</title>
            <authorList>
                <person name="O'Connor C.M."/>
                <person name="Shenk T."/>
            </authorList>
            <dbReference type="PubMed" id="21307184"/>
            <dbReference type="DOI" id="10.1128/JVI.02442-10"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>clinical isolate BFXwtGFP</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Plays an important role in spread of HCMV via the extracellular route. As a G-protein-coupled receptor (vGPCR), may activate signaling pathways important for virion assembly or egress processes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimerizes with US28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Host cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081611.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HDK1"/>
    <dbReference type="PRIDE" id="F5HDK1"/>
    <dbReference type="DNASU" id="3077557"/>
    <dbReference type="GeneID" id="3077557"/>
    <dbReference type="KEGG" id="vg:3077557"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0297">G-protein coupled receptor</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0675">Receptor</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0807">Transducer</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000416442" description="Envelope glycoprotein US27">
        <location>
            <begin position="1"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="2">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="2">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="2">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="2">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="2">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="2">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="2">
        <location>
            <begin position="301"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="21307184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="364" mass="42228" checksum="FC6CE41FB8BBABDD" modified="2011-06-28" version="1">MTTSTTTTTNIMLQVSNVTNHTLNSTEIYQLFEYTRFGVWLMCIVGTFLNMLVITTILYYRRKKKSPSDTYICNLAVADLLIVVGLPFFLEYAKHHPKLSREVVCSGLNACFYICLFAGVCFLINLSMDRYCVIVWGVELNRVRNNKRATCWVVIFWILAALMGMPHYLMYSHTNNECVGEFANETSGWFPVFLNTKVNICGYLAPIVLMAYTYNRMVRFIINYVGKWHMQTLHVLLVVVVSFASFWFPFNLALFLESIRLLSGTQNETLQTVITFCLYVGQFLAYVRACLNPGIYILVGTQMRKDMWTTLRVFACCCVKQEIPYQDIDIELQKDIQRRAKHTKRTHYDRKHAPMESGEEEFLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5HDM3</accession>
    <name>UL23_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Tegument protein UL23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL23</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the inhibition of host innate immune response by disrupting the interaction between NMI and STAT1. In turn, NMI-mediated transcription of interferon-gamma stimulated genes is inhibited.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host NMI; this interaction inhibits NMI interaction with STAT1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae US22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081482.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HDM3"/>
    <dbReference type="DNASU" id="3077524"/>
    <dbReference type="GeneID" id="3077524"/>
    <dbReference type="KEGG" id="vg:3077524"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000416715" description="Tegument protein UL23">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="284" mass="32957" checksum="FE02F65EC1CF22AC" modified="2011-06-28" version="1">MSVIKDCFLNLLDRWRPPKTSRPWKPGQRVALVWPKDRCLVIRRRWRLVRDEGRDAQRLASYLCCPEPLRFVGSICTYNFLKHKGDHNVPSELYLGASGAMYLWTDHIYSDSLTFVAESITEFLNIGLRRCNFITVPEELPHTASLRALAGCMHIHAFAQWRATYRGRLMVMGDYSVIRVSTIRLYDWSEINDWRVMVGSNHVEPLGWLVSPYDVINLFVDDCMRVFAANNQHVCIVADSLMEFVTRGMTRCHENGIYYGTRSMRKLNKPTCPYGVDHQLFDDA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-02-23" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F5HDQ6</accession>
    <name>DNBI_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Major DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">DBP</name>
        <name type="synonym">UL57</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays several crucial roles in viral infection. Participates in the opening of the viral DNA origin to initiate replication by interacting with the origin-binding protein. May disrupt loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation. Promotes viral DNA recombination by performing strand-transfer, characterized by the ability to transfer a DNA strand from a linear duplex to a complementary single-stranded DNA circle. Can also catalyze the renaturation of complementary single strands. Additionally, reorganizes the host cell nucleus, leading to the formation of prereplicative sites and replication compartments. This process is driven by the protein which can form double-helical filaments in the absence of DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooligomers. Forms double-helical filaments necessary for the formation of replication compartments within the host nucleus. Interacts with the origin-binding protein. Interacts with the helicase primase complex; this interaction stimulates primer synthesis activity of the helicase-primase complex. Interacts with the DNA polymerase. Interacts with the alkaline exonuclease; this interaction increases its nuclease processivity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <text evidence="1">In the absence of DNA replication, found in the nuclear framework-associated structures (prereplicative sites). As viral DNA replication proceeds, it migrates to globular intranuclear structures (replication compartments).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae major DNA-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081516.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HDQ6"/>
    <dbReference type="PRIDE" id="F5HDQ6"/>
    <dbReference type="GeneID" id="3077528"/>
    <dbReference type="KEGG" id="vg:3077528"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04007">
        <property type="entry name" value="HSV_DNBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035989">
        <property type="entry name" value="DBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043031">
        <property type="entry name" value="Viral_ssDBP_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000635">
        <property type="entry name" value="Viral_ssDNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00747">
        <property type="entry name" value="Viral_DNA_bp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118208">
        <property type="entry name" value="SSF118208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000416448" description="Major DNA-binding protein">
        <location>
            <begin position="1"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="536"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1214"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Required for nuclear localization" evidence="1">
        <location>
            <begin position="1232"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Required for filament formation" evidence="1">
        <location>
            <begin position="846"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="1235" mass="133849" checksum="19C62999E24AEE8A" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F5HE05</accession>
    <name>US02_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Unique short US2 glycoprotein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein HQLF2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>gpUS2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">US2</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Participates in the inhibition of the host immune response. Early protein that redirects newly synthesized major histocompatibility complex (MHC) class I heavy chains via the SEC61 translocon to the cytosol where they undergo proteasome-dependent destruction. In consequence, infected cells are masked for immune recognition by cytotoxic T lymphocytes. Seems so far to be specific for HLA-A, HLA-B, and HFE loci products. Does not interact with HLA-DR or HLA-DM.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer. Interacts with host TRAM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="developmental stage">
        <text>Expressed at early period of virus infection.</text>
    </comment>
    <comment type="domain">
        <text>The lumenal domain allows tight interaction with class I molecules encoded by the HLA-A locus.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">The signal sequence is not cleaved.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytomegalovirus US2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081589.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HE05"/>
    <dbReference type="BioGRID" id="1678095">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HE05"/>
    <dbReference type="DNASU" id="3077542"/>
    <dbReference type="GeneID" id="3077542"/>
    <dbReference type="KEGG" id="vg:3077542"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044167">
        <property type="term" value="C:host cell endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009237">
        <property type="entry name" value="Herpes_US2/US3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05963">
        <property type="entry name" value="Cytomega_US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0393">Immunoglobulin domain</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="chain" id="PRO_0000418292" description="Unique short US2 glycoprotein">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="signal peptide" description="Not cleaved">
        <location>
            <begin position="1"/>
            <end status="unknown"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="3">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like H-type">
        <location>
            <begin position="43"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="52"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P09713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="199" mass="23112" checksum="201A43BDE87793E6" modified="2011-06-28" version="1">MNNLWKAWVGLWTSMGPLIRLPDGITKAGEDALRPWKSTAKHPWFEIEDNRCYIDNGKLFARGSIVGNMSRFVFDPKADYGGVGENLYVHADDVEFVPGESLKWNVRNLDVMPIFETLALRLVLQGDVIWLRCVPELRVDYTSSAYMWNMQYGMVRKSYTHVAWTIVFYSINITLLVLFIVYVTVDCNLSMMWMRFFVC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F5HE12</accession>
    <name>UL35_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Tegument protein UL35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL35</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <molecule>Isoform UL35</molecule>
        <text evidence="1">Plays important role in immediate-early gene expression through interaction with UL82. Forms nuclear bodies in host nucleus, independently of PML. In turn, UL35 nuclear bodies associate with and remodel PML bodies. Through interaction with host DCAF1, causes cells to accumulate in the G2 phase of the cell cycle by inducing a DNA damage response. Regulates viral assembly by controlling the localization of the essential gB through regulation of a retrograde transport pathway. This modulation occurs via binding and inhibition of host sorting nexin 5/SNX5. Also plays a role in the inhibition of pattern recognition receptor-mediated type I interferon signaling at the level of TBK1.</text>
    </comment>
    <comment type="function">
        <molecule>Isoform UL35A</molecule>
        <text evidence="1">Promotes cytoplasmic UL82 accumulation and inhibits UL35-containing nuclear bodies formation. Regulates viral assembly by controlling the localization of the essential gB through regulation of a retrograde transport pathway. This modulation occurs via binding and inhibition of host sorting nexin 5/SNX5.</text>
    </comment>
    <comment type="subunit">
        <molecule>Isoform UL35</molecule>
        <text evidence="1">Interacts with UL82. Interacts with isoform UL35A. Interacts with host UBP7; this interaction significantly inhibits the ability of USP7 to form nuclear bodies. Interacts with host DCAF1 (via C-terminus). Interacts with host SNX5; this interaction allows proper gB localization during viral assembly. Interacts wth host TBK1; this interaction prevents type I interferon production.</text>
    </comment>
    <comment type="subunit">
        <molecule>Isoform UL35A</molecule>
        <text evidence="1">Interacts with UL82. Interacts with isoform UL35. Interacts with host UBP7; this interaction significantly inhibits the ability of USP7 to form nuclear bodies. Interacts with host SNX5; this interaction allows proper gB localization during viral assembly.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Isoform UL35</molecule>
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Found in nuclear bodies.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Isoform UL35A</molecule>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="alternative initiation"/>
        <isoform>
            <id>F5HE12-1</id>
            <name>UL35</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>F5HE12-2</id>
            <name>UL35A</name>
            <sequence type="described" ref="VSP_044021"/>
        </isoform>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae pp85 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081494.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HE12"/>
    <dbReference type="BioGRID" id="1677983">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HE12"/>
    <dbReference type="DNASU" id="3077429"/>
    <dbReference type="GeneID" id="3077429"/>
    <dbReference type="KEGG" id="vg:3077429"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030430">
        <property type="term" value="C:host cell cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039695">
        <property type="term" value="P:DNA-templated viral transcription"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039722">
        <property type="term" value="P:suppression by virus of host toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039723">
        <property type="term" value="P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006731">
        <property type="entry name" value="Herpes_pp85"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04637">
        <property type="entry name" value="Herpes_pp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0024">Alternative initiation</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-1223">Inhibition of host TBK1 by virus</keyword>
    <keyword id="KW-1225">Inhibition of host TLR pathway by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000418284" description="Tegument protein UL35">
        <location>
            <begin position="1"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="500"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="587"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="500"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="588"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="626"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_044021" description="In isoform UL35A." evidence="3">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="641" mass="72630" checksum="0347DFCF557C10F6" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5HE44</accession>
    <name>US12_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein US12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US12</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytomegalovirus US12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081597.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HE44"/>
    <dbReference type="DNASU" id="3077562"/>
    <dbReference type="GeneID" id="3077562"/>
    <dbReference type="KEGG" id="vg:3077562"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418293" description="Uncharacterized protein US12">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="281" mass="32471" checksum="22F7CD9FD7F4B70F" modified="2011-06-28" version="1">MVQIQFHQGEPLGHKKEKPPPVSPPSPPPIRRVTVITKDEDTLRSVQHFLWMVRLYGTVVFQTSATIATTILFMLIPWRVTTPYLRDTLPFWSTLLPCALRCHAYWLERQRRPGTLMLVMVYTTLTTISVSTIGLCFDRTVVIQAYVLSSMLCVWCTGLAWLMAWNMQRRLAILCLLSFMLPILWLFIAVQSWEPYQRIILALTVSFIYGLKIVLIRDTLTVLYRSPSNCYTDGDLLRTAMLLYMDQVIMFLLVVVPLTAPIWYPNYAGALGRTAHWLFHK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>F5HE74</accession>
    <name>U148A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL148A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL148A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081582.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HE74"/>
    <dbReference type="PRIDE" id="F5HE74"/>
    <dbReference type="DNASU" id="3077461"/>
    <dbReference type="GeneID" id="3077461"/>
    <dbReference type="KEGG" id="vg:3077461"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033644">
        <property type="term" value="C:host cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418327" description="Protein UL148A">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="80" mass="9062" checksum="2589CC50825DFE31" modified="2011-06-28" version="1">MSSDSNFDPWIPVCVVVVMTSVVLFAGLHVYLWYVRRQLVAFCLEKVCVRCCGKDETTPLVEDAEPPAELEMVEVSDECY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F5HEN8</accession>
    <name>HELI_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA replication helicase</fullName>
            <ecNumber evidence="1">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">HELI</name>
        <name type="ordered locus">UL105</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs. Possesses helicase-like motifs and therefore may act as the helicase subunit of the complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with the primase and the primase-associated factor to form the helicase-primase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="1"/>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081551.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HEN8"/>
    <dbReference type="GeneID" id="3077546"/>
    <dbReference type="KEGG" id="vg:3077546"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039686">
        <property type="term" value="P:bidirectional double-stranded viral DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04030">
        <property type="entry name" value="HSV_HELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003840">
        <property type="entry name" value="DNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034711">
        <property type="entry name" value="HSV_HELI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02689">
        <property type="entry name" value="Herpes_Helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418233" description="DNA replication helicase">
        <location>
            <begin position="1"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="658"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="660"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="120"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="956" mass="106508" checksum="3196BB7A55BFE9F4" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F5HET1</accession>
    <name>UL78_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL78</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL78</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Arch. Virol." volume="157" first="935" last="949">
            <title>The 7-transmembrane protein homologue UL78 of the human cytomegalovirus forms oligomers and traffics between the plasma membrane and different intracellular compartments.</title>
            <authorList>
                <person name="Wagner S."/>
                <person name="Arnold F."/>
                <person name="Wu Z."/>
                <person name="Schubert A."/>
                <person name="Walliser C."/>
                <person name="Tadagaki K."/>
                <person name="Jockers R."/>
                <person name="Mertens T."/>
                <person name="Michel D."/>
            </authorList>
            <dbReference type="PubMed" id="22327422"/>
            <dbReference type="DOI" id="10.1007/s00705-012-1246-6"/>
        </citation>
        <scope>OLIGOMERIZATION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2012" name="Blood" volume="119" first="4908" last="4918">
            <title>Human cytomegalovirus-encoded UL33 and UL78 heteromerize with host CCR5 and CXCR4 impairing their HIV coreceptor activity.</title>
            <authorList>
                <person name="Tadagaki K."/>
                <person name="Tudor D."/>
                <person name="Gbahou F."/>
                <person name="Tschische P."/>
                <person name="Waldhoer M."/>
                <person name="Bomsel M."/>
                <person name="Jockers R."/>
                <person name="Kamal M."/>
            </authorList>
            <dbReference type="PubMed" id="22496149"/>
            <dbReference type="DOI" id="10.1182/blood-2011-08-372516"/>
        </citation>
        <scope>INTERACTION WITH HOST CCR5 AND CXCR4</scope>
    </reference>
    <comment type="function">
        <text>Acts as a cellular chemokine receptor and may modulate the host cell migration pattern which is the primary cellular event initiated by chemokine receptor activation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomerizes. Interacts with host CCR5 and CXCR4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Host endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text>Once localized on the cell surface, UL78 is quickly endocytosed.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081526.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HET1"/>
    <dbReference type="PRIDE" id="F5HET1"/>
    <dbReference type="DNASU" id="3077549"/>
    <dbReference type="GeneID" id="3077549"/>
    <dbReference type="KEGG" id="vg:3077549"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0297">G-protein coupled receptor</keyword>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0675">Receptor</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0807">Transducer</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418318" description="Protein UL78">
        <location>
            <begin position="1"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="109"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="22327422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="22496149"/>
        </source>
    </evidence>
    <sequence length="431" mass="47357" checksum="34668FE7F908C657" modified="2011-06-28" version="1">MSPSVEETTSVTESIMFAIVSFKHMGPFEGYSMSADRAASDLLIGMFGSVSLVNLLTIIGCLWVLRVTRPPVSVMIFTWNLVLSQFFSILATMLSKGIMLRGALNLSLCRLVLFVDDVGLYSTALFFLFLILDRLSAISYGRDLWHHETRENAGVALYAVAFAWVLSIVAAVPTAATGSLDYRWLGCQIPIQYAAVDLTIKMWFLLGAPMIAVLANVVELAYSDRRDHVWSYVGRVCTFYVTCLMLFVPYYCFRVLRGVLQPASAAGTGFGIMDYVELATRTLLTMRLGILPLFIIAFFSREPTKDLDDSFDYLVERCQQSCHGHFVRRLVQALKRAMYSVELAVCYFSTSVRDVAEAVKKSSSRCYADATSAAVVVTTTTSEKATLVEHAEGMASEMCPGTTIDVSAESSSVLCTDGENTVASDATVTAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5HF35</accession>
    <name>UL136_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL136</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL136</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Braz. J. Med. Biol. Res." volume="44" first="1251" last="1255">
            <title>Interaction between human cytomegalovirus UL136 protein and ATP1B1 protein.</title>
            <authorList>
                <person name="Cui X."/>
                <person name="Sun Z.R."/>
                <person name="Ren G.W."/>
                <person name="Wang G.L."/>
                <person name="Qi Y."/>
                <person name="Ma Y.P."/>
                <person name="Ruan Q."/>
            </authorList>
            <dbReference type="PubMed" id="22030864"/>
            <dbReference type="DOI" id="10.1590/S0100-879X2011007500144"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>INTERACTION WITH HOST ATP1B1</scope>
        <source>
            <strain>H</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with host ATP1B1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HHV-5 UL136 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081579.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HF35"/>
    <dbReference type="DNASU" id="3077568"/>
    <dbReference type="GeneID" id="3077568"/>
    <dbReference type="KEGG" id="vg:3077568"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000417857" description="Protein UL136">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="22030864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="240" mass="27060" checksum="3A21408C8F2E29D2" modified="2011-06-28" version="1">MSVKGVEMPEMTWDLDVGNKWRRRKVLSRIHRFWECRLRVWWLSDAGVRETDPPRPRRRPTWMTAVFHVICAVLLTLMIMAIGALIAYLRYYHQDSWRDMLHDLFCGCHYPEKCRRHHERQRSRRRAMDVPDPELGDPARRPLNGAMYYGSGCRFDTVEMVDETRPAPPALSSPETGDDSNDDAVAGGGAGGVTSSATRTTSSNALLPEWMDAVHVAVQAAVQATVQVSGPRENAVSPAT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F5HF62</accession>
    <name>US28_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Envelope protein US28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US28</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor for a C-C type chemokine. Binds to a great number of different CC-chemokines including CCL5/RANTES, CCL2/MCP-1, CCL3/MIP-1-alpha as well as CX3CL1/Fractalkine. Transduces signals resulting in the activation of MAP kinase signaling pathways and augmentation of intracellular calcium ion levels, leading to alterations in chemotactic behavior of vascular smooth muscle cells and macrophages. The US28 receptor also exhibits high levels of agonist-independent signaling activity and agonist-independent endocytosis. Interacts with the host Gi complex without activating it, thereby probably interfering with the chemokine-Gi signaling. May also function as a G protein sink to sequester G protein from the cell surface via internalization. Interacts with endogenous Gaq/11 subunits and thereby constitutively activates phospholipase C.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host GPRASP1; this interaction targets US28 to lysosomes for degradation (By similarity). Interacts with host CX3CL1/Fractalkine (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated. High phosphorylation occurs concomitantly with receptor endocytosis and correlate with low receptor presence at the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081612.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HF62"/>
    <dbReference type="PRIDE" id="F5HF62"/>
    <dbReference type="DNASU" id="3077536"/>
    <dbReference type="GeneID" id="3077536"/>
    <dbReference type="KEGG" id="vg:3077536"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004950">
        <property type="term" value="F:chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039553">
        <property type="term" value="P:suppression by virus of host chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0297">G-protein coupled receptor</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1086">Inhibition of host chemokines by virus</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0675">Receptor</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0807">Transducer</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="chain" id="PRO_0000418264" description="Envelope protein US28">
        <location>
            <begin position="1"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="295"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P69332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00521"/>
        </source>
    </evidence>
    <sequence length="354" mass="41034" checksum="3C1B7B7C54DF7AAD" modified="2011-06-28" version="1">MTPTTTTAELTTEFDYDEDATPCVFTDVLNQSKPVTLFLYGVVFLFGSIGNFLVIFTITWRRRIQCSGDVYFINLAAADLLFVCTLPLWMQYLLDHNSLASVPCTLLTACFYVAMFASLCFITEIALDRYYAIVYMRYRPVKQACLFSIFWWIFAVIIAIPHFMVVTKKDNQCMTDYDYLEVSYPIILNVELMLGAFVIPLSVISYCYYRISRIVAVSQSRHKGRIVRVLIAVVLVFIIFWLPYHLTLFVDTLKLLKWISSSCEFERSLKRALILTESLAFCHCCLNPLLYVFVGTKFRQELHCLLAEFRQRLFSRDVSWYHSMSFSRRGSPSRRETSSDTLSDEVCRVSQIIP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F5HFH0</accession>
    <name>US15_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane protein US15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US15</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HHV-5 US12 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081600.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HFH0"/>
    <dbReference type="DNASU" id="3077565"/>
    <dbReference type="GeneID" id="3077565"/>
    <dbReference type="KEGG" id="vg:3077565"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418253" description="Membrane protein US15">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="262" mass="29099" checksum="42103A97A043EC5C" modified="2011-06-28" version="1">MRREKGFQVPTDGTVIYVPPGIQETRLATRSLAWVDCCRVALHTYGAVGWQLAGLTALLSAFCYAAPATWFHHSRLCLTESSPSLVFVIPVTSVIFIHCYETSHPSNIGVLLFYTLLHVPPLIVICLCLDGTLVISAALFTLLAFLSCTGVALLAPERTVRRQIVVIHALITLTFTAIVVVILRRGWSWCFKIVLSFSVLITCLAVSHFHEAALAVRYETPLERALLAAVKVFLSLVFTLLMVLRIMTLRTFLQTYFSSDKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F5HFJ7</accession>
    <name>US10_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane glycoprotein US10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US10</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text>Plays a role in the modulation of host immune response by down-regulating the surface presentation of HLA-G molecules. Selectively targets HLA-G molecules for degradation by a mechanism distinct from the one used by US11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 US6 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081595.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HFJ7"/>
    <dbReference type="DNASU" id="3077551"/>
    <dbReference type="GeneID" id="3077551"/>
    <dbReference type="KEGG" id="vg:3077551"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044167">
        <property type="term" value="C:host cell endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035128">
        <property type="entry name" value="US10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17617">
        <property type="entry name" value="US10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0393">Immunoglobulin domain</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418251" description="Membrane glycoprotein US10">
        <location>
            <begin position="25"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="25"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like H-type">
        <location>
            <begin position="30"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="39"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="185" mass="20771" checksum="43B67DA189A5EF93" modified="2011-06-28" version="1" precursor="true">MLRRGSLRNPLAICLLWWLGVVAAATEETREPTYFTCGCVIQNHVLKGAVKLYGQFPSPKTLRASAWLHDGENHERHRQPILVEGTATATEALYILLPTELSSPEGNRPRNYSATLTLASRDCYERFVCPVYDSGTPMGVLMNLTYLWYLGDYGAILKIYFGLFCGACVITRSLLLICGYYPPRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5HFJ8</accession>
    <name>UL91_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL91</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL91</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae UL91 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081538.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="DNASU" id="3077494"/>
    <dbReference type="GeneID" id="3077494"/>
    <dbReference type="KEGG" id="vg:3077494"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035385">
        <property type="entry name" value="U62/UL91"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17442">
        <property type="entry name" value="U62_UL91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418250" description="Protein UL91">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="111" mass="12114" checksum="43B8C39BB35634D3" modified="2011-06-28" version="1">MNSLLAELNRLGVAHATTEDVFIFVDRLFQHFSFLFQAEESGPRRLELVASVFEHLTVECVNDILDACSHPDVNVVETSNTCRPCPSPVPSAPKTVSDAQTSCATPRAPVT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-02-23" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F5HG51</accession>
    <name>PRIM_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase</fullName>
            <ecNumber evidence="1">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL70</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase initiates primer synthesis and thereby produces large amount of short RNA primers on the lagging strand that the polymerase elongates using dNTPs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with the helicase and the primase-associated factor to form the helicase-primase factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <text evidence="1">Requires the presence of the primase associated factor to properly localize in the host cell nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae DNA primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="1"/>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081518.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HG51"/>
    <dbReference type="GeneID" id="3077484"/>
    <dbReference type="KEGG" id="vg:3077484"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04011">
        <property type="entry name" value="HSV_PRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033685">
        <property type="entry name" value="HSV_PRIM"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000416450" description="DNA primase">
        <location>
            <begin position="1"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CHC2-type" evidence="1">
        <location>
            <begin position="881"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="596"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="site" description="Essential for primase activity" evidence="1">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <feature type="site" description="Essential for primase activity" evidence="1">
        <location>
            <position position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="946" mass="107842" checksum="0818168F70714FAC" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F5HG68</accession>
    <name>UL16P_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL16</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the escape of host immune response. Blocks the interaction between the host KLRK1 receptor with their ligand ULBP1 and ULBP2. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Therefore, the sequestration of diverse KLRK1 ligands in the endoplasmic reticulum and cis-Golgi apparatus of cells by UL16 inhibits the activation of NK cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host ULBP1, ULBP2 and MICB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 UL16 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081475.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HG68"/>
    <dbReference type="PRIDE" id="F5HG68"/>
    <dbReference type="DNASU" id="3077464"/>
    <dbReference type="GeneID" id="3077464"/>
    <dbReference type="KEGG" id="vg:3077464"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038671">
        <property type="entry name" value="HCMV_UL16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035123">
        <property type="entry name" value="UL16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17622">
        <property type="entry name" value="UL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418245" description="Protein UL16">
        <location>
            <begin position="22"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="22"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="230" mass="26187" checksum="51E1ACED95265011" modified="2011-06-28" version="1" precursor="true">MERRRGTVPLGWVFFVLCLSASSPCAVDLGSKSSNSTCRLNVTELASIHPGETWTLHGMCISICYYENVTEDEIIGVAFTWQHNESVVDLWLYQNDTVIRNFSDITTNILQDGLKMRTVPVTKLYTSRMVTNLTVGRYDCLRCENGTMKIIERLYVRLGSLYPRPPGSGLAKHPSVSADEELSATLARDIVLVSAITLFFFLLALRIPQRLCQRLRIRLPHRYQRLRTED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>F5HG95</accession>
    <name>US29_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein US29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US29</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HHV-5 US29 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081613.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HG95"/>
    <dbReference type="DNASU" id="3077483"/>
    <dbReference type="GeneID" id="3077483"/>
    <dbReference type="KEGG" id="vg:3077483"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418255" description="Uncharacterized protein US29">
        <location>
            <begin position="1"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="462" mass="51034" checksum="531C56FCEC19F746" modified="2011-06-28" version="1">MRCFRWWLYSGWWWLTFGCARTVTVGFVAPTVRAQSTVVRSEPAPPSETRRDNNDTSYFSSTSFHSSVSPATSVDRQFRRTTYDRWDGRRWLRTRYGNASACVTGTQWSTNFFFSQCEHYPSFVKLNGVQRWTPVRRPMGEVAYYGGCCMVGGGNRAYVILVSGYGTASYGNALRVDFGRGNCTAPKRTYPRRLELHDGRTDPSRCDPYQVYFYGLQCPEQLVITAHGGVGMRRCPTGSRPTPSRPHRHDLENELHGLCVDLLVCVLLLALLLLELVPMEAVRHPLLFWRRVALSPSTSKVDRAVKLCLRRMLGLPPPPSVAPPGEKKELPAQAALSPPLTTWSLPPFPSTRIPDSPPPPYQLRHATSLVTVPTLLLYTSSDIGDTASETTCVAHATYGEPPEPARSTATVQECTVLTAPNCGIVNNDGAVSEGQDHGDAVHHSLDVVSQCAADTGVVDTSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5HG98</accession>
    <name>UL38_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Apoptosis inhibitor UL38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL38</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="J. Virol." volume="81" first="3109" last="3123">
            <title>Human cytomegalovirus UL38 protein blocks apoptosis.</title>
            <authorList>
                <person name="Terhune S."/>
                <person name="Torigoi E."/>
                <person name="Moorman N."/>
                <person name="Silva M."/>
                <person name="Qian Z."/>
                <person name="Shenk T."/>
                <person name="Yu D."/>
            </authorList>
            <dbReference type="PubMed" id="17202209"/>
            <dbReference type="DOI" id="10.1128/JVI.02124-06"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="Cell Host Microbe" volume="3" first="253" last="262">
            <title>Human cytomegalovirus protein UL38 inhibits host cell stress responses by antagonizing the tuberous sclerosis protein complex.</title>
            <authorList>
                <person name="Moorman N.J."/>
                <person name="Cristea I.M."/>
                <person name="Terhune S.S."/>
                <person name="Rout M.P."/>
                <person name="Chait B.T."/>
                <person name="Shenk T."/>
            </authorList>
            <dbReference type="PubMed" id="18407068"/>
            <dbReference type="DOI" id="10.1016/j.chom.2008.03.002"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HOST TSC2</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2011" name="J. Virol." volume="85" first="9103" last="9113">
            <title>The human cytomegalovirus protein pUL38 suppresses endoplasmic reticulum stress-mediated cell death independently of its ability to induce mTORC1 activation.</title>
            <authorList>
                <person name="Qian Z."/>
                <person name="Xuan B."/>
                <person name="Gualberto N."/>
                <person name="Yu D."/>
            </authorList>
            <dbReference type="PubMed" id="21715486"/>
            <dbReference type="DOI" id="10.1128/JVI.00572-11"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2018" name="J. Virol." volume="0" first="0" last="0">
            <title>Human Cytomegalovirus Protein pUL38 Prevents Premature Cell Death by Binding to Ubiquitin-specific Protease 24 and Regulating Iron Metabolism.</title>
            <authorList>
                <person name="Sun Y."/>
                <person name="Bao Q."/>
                <person name="Xuan B."/>
                <person name="Xu W."/>
                <person name="Pan D."/>
                <person name="Li Q."/>
                <person name="Qian Z."/>
            </authorList>
            <dbReference type="PubMed" id="29695420"/>
            <dbReference type="DOI" id="10.1128/JVI.00191-18"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HOST USP24</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3 4 5 6">Plays a role in the inhibition of host apoptosis to facilitate efficient viral replication. Promotes stabilization and inactivation of host TP53 through interaction with host MDM2 (By similarity). Induces host mTORC1 activation by antagonizing the ability of host TSC1/2 to negatively regulate mTORC1. Thus, inhibits a growth regulatory pathway to facilitate viral replication. Prevents premature cell host cell death by blocking host ubiquitin-specific protease 24/USP24-mediated autophagic ferritin degradation in lysosomes thus maintaining lysosome integrity and cellular viability (PubMed:29695420).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 4">Interacts with host MDM2; this interaction leads to the stabilization of host TP53 (By similarity). Interacts with host TSC2; this interaction prevents host cell stress responses. Interacts with host USP24.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Host nucleus</location>
        </subcellularLocation>
        <text>localized in the host nucleus at early times postinfection and then increases in both nuclear and cytoplasmic compartments during the course of infection.</text>
    </comment>
    <comment type="similarity">
        <text>Belongs to the beta-herpesvirinae UL38 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081497.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HG98"/>
    <dbReference type="DNASU" id="3077531"/>
    <dbReference type="GeneID" id="3077531"/>
    <dbReference type="KEGG" id="vg:3077531"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1119">Modulation of host cell apoptosis by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417845" description="Apoptosis inhibitor UL38">
        <location>
            <begin position="1"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9WT45"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="17202209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="18407068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="21715486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="29695420"/>
        </source>
    </evidence>
    <sequence length="331" mass="36753" checksum="533D3F344DDBBB54" modified="2011-06-28" version="1">MTTTTHSTAAIMSLLDEAEWRQTQMDVGGLIQASALGKVALRYAVRKLMKRGARLRHDSGLYVCICDPSYEFLQMNLSKISWLERHCPPLDQELIMFGVIEAWEEASVRPTRQLVLFMTPKWDVFAYDSGILFFLAPSMAQFWHGAIVLEYWNALFPVEVRSHVRQHAHTMDDLVMVFHQLDYEKQVLEARRDKNTEGPRTFAKSVNSYVRAILESERRIREGKIPMTFVDRDSLRANSLAHIQATGAQPSHAPAQRVLSAPPSLPSPVSEEDPAAAATPPSSAATTPPSSVVPASVESELSSSPPLPPVVVKDVMYTAGEGDVVQMVVVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F5HGG3</accession>
    <name>UL26_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Tegument protein UL26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL26</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="J. Virol." volume="80" first="3541" last="3548">
            <title>UL26-deficient human cytomegalovirus produces virions with hypophosphorylated pp28 tegument protein that is unstable within newly infected cells.</title>
            <authorList>
                <person name="Munger J."/>
                <person name="Yu D."/>
                <person name="Shenk T."/>
            </authorList>
            <dbReference type="PubMed" id="16537622"/>
            <dbReference type="DOI" id="10.1128/JVI.80.7.3541-3548.2006"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2014" name="J. Virol." volume="88" first="14289" last="14300">
            <title>The human cytomegalovirus UL26 protein antagonizes NF-kappaB activation.</title>
            <authorList>
                <person name="Mathers C."/>
                <person name="Schafer X."/>
                <person name="Martinez-Sobrido L."/>
                <person name="Munger J."/>
            </authorList>
            <dbReference type="PubMed" id="25275128"/>
            <dbReference type="DOI" id="10.1128/JVI.02552-14"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Plays a role in the inhibition of host NF-kappa-B. This inhibition affects both the canonical and the non-canonical pathways. Blocks the induction of host IKK phosphorylation. May also influence the normal phosphorylation state of several tegument proteins including pp28 in virions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host nucleus</location>
        </subcellularLocation>
        <text>Also found in dense bodies.</text>
    </comment>
    <comment type="alternative products">
        <event type="alternative initiation"/>
        <isoform>
            <id>F5HGG3-1</id>
            <name>27kDa</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>F5HGG3-2</id>
            <name>21kDa</name>
            <sequence type="described" ref="VSP_043922"/>
        </isoform>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the herpesviridae US22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081485.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HGG3"/>
    <dbReference type="DNASU" id="3077445"/>
    <dbReference type="GeneID" id="3077445"/>
    <dbReference type="KEGG" id="vg:3077445"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-0024">Alternative initiation</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000417843" description="Tegument protein UL26">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_043922" description="In isoform 21kDa." evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16537622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="25275128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="222" mass="24867" checksum="8CC0B9D429C6C02E" modified="2012-06-13" version="2">MYAVFGLTRSEVTPHEAAAARYKHRGVIDRRGAAMTSRRAPDGGLNLDDFMRRQRGRHLDLPYPRGYTLFVCDVEETILTPRDVEYWKLLVVTQGQLRVIGTIGLANLFSWDRSVAGVAADGSVLCYEISRENFVVRAADSLPQLLERGLLHSYFEDVERAAQGRLRHGNRSGLRRDADGQVIRESACYVSRALLRHRVTPGKQEITDAMFEAGNVPSALLP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F5HGI9</accession>
    <name>TRM2_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tripartite terminase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">TRM2</name>
        <name type="ordered locus">UL51</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the molecular motor that translocates viral genomic DNA in empty capsid during DNA packaging. Forms a tripartite terminase complex together with TRM1 and TRM3 in the host cytoplasm. Once the complex reaches the host nucleus, it interacts with the capsid portal vertex. This portal forms a ring in which genomic DNA is translocated into the capsid.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with TRM1 and TRM3 to form the tripartite terminase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <text evidence="1">Found associated with the external surface of the viral capsid during assembly and DNA packaging, but seems absent in extracellular mature virions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae TRM2 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081510.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HGI9"/>
    <dbReference type="DrugBank" id="DB12070">
        <property type="generic name" value="Letermovir"/>
    </dbReference>
    <dbReference type="DrugCentral" id="F5HGI9"/>
    <dbReference type="PRIDE" id="F5HGI9"/>
    <dbReference type="DNASU" id="3077482"/>
    <dbReference type="GeneID" id="3077482"/>
    <dbReference type="KEGG" id="vg:3077482"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04015">
        <property type="entry name" value="HSV_TRM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005208">
        <property type="entry name" value="Herpes_TT2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03581">
        <property type="entry name" value="Herpes_UL33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0231">Viral genome packaging</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <feature type="chain" id="PRO_0000418249" description="Tripartite terminase subunit 2">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="157" mass="16978" checksum="5999035AC484517D" modified="2011-06-28" version="1">MSWAKQRVPFLDDDDGEEENDVQDDVDSPVPTRPLVIDEDAEPAAGTSGGLEGGGGDDEDGEDGHALPDLDDDLLLQFEPMLPRVYDLLLPSLDARLNFVNAGQKYAAFLKYVHGDCATCSHGEILREKTQLLTAIVSKLMDINGILEGKDEPAPGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F5HGQ8</accession>
    <name>UL138_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL138</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL138</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="Blood" volume="110" first="937" last="945">
            <title>Human cytomegalovirus sequences expressed in latently infected individuals promote a latent infection in vitro.</title>
            <authorList>
                <person name="Goodrum F."/>
                <person name="Reeves M."/>
                <person name="Sinclair J."/>
                <person name="High K."/>
                <person name="Shenk T."/>
            </authorList>
            <dbReference type="PubMed" id="17440050"/>
            <dbReference type="DOI" id="10.1182/blood-2007-01-070078"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="J. Virol." volume="83" first="5615" last="5629">
            <title>Characterization of a novel Golgi apparatus-localized latency determinant encoded by human cytomegalovirus.</title>
            <authorList>
                <person name="Petrucelli A."/>
                <person name="Rak M."/>
                <person name="Grainger L."/>
                <person name="Goodrum F."/>
            </authorList>
            <dbReference type="PubMed" id="19297488"/>
            <dbReference type="DOI" id="10.1128/JVI.01989-08"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>FIXwt</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2011" name="J. Virol." volume="85" first="11409" last="11421">
            <title>The latency-associated UL138 gene product of human cytomegalovirus sensitizes cells to tumor necrosis factor alpha (TNF-alpha) signaling by upregulating TNF-alpha receptor 1 cell surface expression.</title>
            <authorList>
                <person name="Montag C."/>
                <person name="Wagner J.A."/>
                <person name="Gruska I."/>
                <person name="Vetter B."/>
                <person name="Wiebusch L."/>
                <person name="Hagemeier C."/>
            </authorList>
            <dbReference type="PubMed" id="21880774"/>
            <dbReference type="DOI" id="10.1128/JVI.05028-11"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2011" name="J. Virol." volume="85" first="13260" last="13270">
            <title>The cytomegaloviral protein pUL138 acts as potentiator of tumor necrosis factor (TNF) receptor 1 surface density to enhance ULb'-encoded modulation of TNF-alpha signaling.</title>
            <authorList>
                <person name="Le V.T."/>
                <person name="Trilling M."/>
                <person name="Hengel H."/>
            </authorList>
            <dbReference type="PubMed" id="21976655"/>
            <dbReference type="DOI" id="10.1128/JVI.06005-11"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HOST TNFR1</scope>
        <source>
            <strain>Toledo</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 5 6">Plays a role in the maintenance of a latent infection in low-passage strains. Additionally, sensitizes infected cells to tumor necrosis factor alpha (TNF-alpha) signaling. Increases host TNFR1 stability and cell surface expression thus enhancing its signaling responses, leading to TNFR1 hyper-responsiveness. By up-regulating TNFR1 surface availability, UL138 may also promote HCMV reactivation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with host TNFR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Host Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081578.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HGQ8"/>
    <dbReference type="PRIDE" id="F5HGQ8"/>
    <dbReference type="DNASU" id="3077420"/>
    <dbReference type="GeneID" id="3077420"/>
    <dbReference type="KEGG" id="vg:3077420"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418301" description="Protein UL138">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="17440050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="19297488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="21880774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="21976655"/>
        </source>
    </evidence>
    <sequence length="169" mass="19309" checksum="5D6A82346DDE357A" modified="2011-06-28" version="1">MDDLPLNVGLPIIGVMLVLIVAILCYLAYHWHDTFKLVRMFLSYRWLIRCCELYGEYERRFADLSSLGLGAVRRESDRRYRFSERPDEILVRWEEVSSQCSYASSRITDRRAGSSSSSSVHVANQRNSVPPPDMAVTAPLTDVDLLKPVTGSATQFTTVAMVHYHQEYT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F5HGT1</accession>
    <name>MCP_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Major capsid protein</fullName>
            <shortName evidence="1">MCP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">MCP</name>
        <name type="synonym">UL86</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Self-assembles to form an icosahedral capsid with a T=16 symmetry, about 200 nm in diameter, and consisting of 150 hexons and 12 pentons (total of 162 capsomers). Hexons form the edges and faces of the capsid and are each composed of six MCP molecules. In contrast, one penton is found at each of the 12 vertices. Eleven of the pentons are MCP pentamers, while the last vertex is occupied by the portal complex. The capsid is surrounded by a layer of proteinaceous material designated the tegument which, in turn, is enclosed in an envelope of host cell-derived lipids containing virus-encoded glycoproteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homomultimer. Makes the hexons and eleven out of twelve pentons. Interacts with triplex proteins 1/TRX1 and 2/TRX2; adjacent capsomers are linked together in groups of three by triplexes, heterotrimeric complexes composed of one molecule of TRX1 and two molecules of TRX2. Interacts with scaffold protein; this interaction allows efficient MCP transport to the host nucleus. Interacts with capsid vertex component 2/CVC2. Interacts with the small capsomere-interacting protein/SCP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae major capsid protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081534.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HGT1"/>
    <dbReference type="PRIDE" id="F5HGT1"/>
    <dbReference type="GeneID" id="3077538"/>
    <dbReference type="KEGG" id="vg:3077538"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04016">
        <property type="entry name" value="HSV_MCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000912">
        <property type="entry name" value="Herpes_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023233">
        <property type="entry name" value="Herpes_MCP_upper_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03122">
        <property type="entry name" value="Herpes_MCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00235">
        <property type="entry name" value="HSVCAPSIDMCP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103417">
        <property type="entry name" value="SSF103417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1147">T=16 icosahedral capsid protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000418237" description="Major capsid protein">
        <location>
            <begin position="1"/>
            <end position="1370"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04016"/>
        </source>
    </evidence>
    <sequence length="1370" mass="153872" checksum="5EEFCEE4D7498E8D" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F5HH39</accession>
    <name>UL21A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL21A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL21A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Required for efficient viral DNA synthesis and the late accumulation of viral IE transcripts.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HHV-5 UL21A protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081480.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BioGRID" id="1678001">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HH39"/>
    <dbReference type="DNASU" id="3077448"/>
    <dbReference type="GeneID" id="3077448"/>
    <dbReference type="KEGG" id="vg:3077448"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035112">
        <property type="entry name" value="UL21a"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17636">
        <property type="entry name" value="UL21a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418279" description="Uncharacterized protein UL21A">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="123" mass="14275" checksum="6596F9899D94F33D" modified="2011-06-28" version="1">MGGSPVPQLTTVTQGLMPSVRMDFRARRPLRRLAFYAPRARRRLFQNHIHPEQRRVLVGEGDEEMLPDLPMEIDIVIDRPPQQPLPNPLVLLLDDVPPHVPGFAPYRVPRPHPMIPEEHWDQF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F5HHL7</accession>
    <name>U148D_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL148D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL148D</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081585.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HHL7"/>
    <dbReference type="PRIDE" id="F5HHL7"/>
    <dbReference type="DNASU" id="3077436"/>
    <dbReference type="GeneID" id="3077436"/>
    <dbReference type="KEGG" id="vg:3077436"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418263" description="Protein UL148D">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="62" mass="7034" checksum="0B5227F7F9BC2255" modified="2011-06-28" version="1">MTAPKCVTTTTYLVKTKEQPWWPDNAIRRWWISVAIVIFIGVCLVALMYFTQQQARNGSGSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F5HHQ0</accession>
    <name>GN_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Envelope glycoprotein N</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gN</name>
        <name type="synonym">UL73</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Envelope glycoprotein necessary for proper maturation of gM and modulation of its membrane fusion activity. Also plays a critical role in virion morphogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts (via N-terminus) with gM (via N-terminus). The gM-gN heterodimer forms the gCII complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host Golgi apparatus</location>
            <location evidence="2">Host trans-Golgi network</location>
        </subcellularLocation>
        <text evidence="2">When coexpressed with gM, localizes in the host trans-Golgi network.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">O-glycosylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae glycoprotein N family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081521.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HHQ0"/>
    <dbReference type="DNASU" id="3077417"/>
    <dbReference type="GeneID" id="3077417"/>
    <dbReference type="KEGG" id="vg:3077417"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044177">
        <property type="term" value="C:host cell Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033644">
        <property type="term" value="C:host cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019031">
        <property type="term" value="C:viral envelope"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055036">
        <property type="term" value="C:virion membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04037">
        <property type="entry name" value="HSV_GN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021003">
        <property type="entry name" value="Cytomegalovirus_glycopN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005211">
        <property type="entry name" value="Herpes_glycoprotein_N_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034707">
        <property type="entry name" value="HSV_GN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03554">
        <property type="entry name" value="Herpes_UL73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12522">
        <property type="entry name" value="UL73_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416451" description="Envelope glycoprotein N" evidence="2">
        <location>
            <begin position="20"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="2">
        <location>
            <begin position="20"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="2">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="21"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (with gM)" evidence="2">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="135" mass="14469" checksum="72FFFE83D5DD676C" modified="2011-06-28" version="1" precursor="true">MEWNTLVLGLLVLSVVASSNNTSTASTPRPSSSTHASTTVKATTVATTSTTTATSTSSTTSAKPGSTTHDPNVMRPHAHNDFYNAHCTSHMYELSLSSFAAWWTMLNALILMGAFCIVLRHCCFQNFTATTTKGY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F5HHT4</accession>
    <name>UL17_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL17</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HHV-5 UL17 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081476.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HHT4"/>
    <dbReference type="DNASU" id="3077497"/>
    <dbReference type="GeneID" id="3077497"/>
    <dbReference type="KEGG" id="vg:3077497"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020527">
        <property type="entry name" value="Cytomegalovir_UL17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17640">
        <property type="entry name" value="UL17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417841" description="Protein UL17">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="104" mass="12672" checksum="7549886F5AE119D0" modified="2011-06-28" version="1">MDHALFTHFVGRPRHCRLEMLILDEQVSKRSWDTTVYHRRRKHLPRRRAPCGPQRPAEIPKRRKKAAVLLFWHDLCWLFRRLFFPREDSEPLMSDPARSPEEEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5HHT6</accession>
    <name>US21_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane protein US21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US21</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytomegalovirus US12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081606.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="TCDB" id="1.A.14.3.12">
        <property type="family name" value="the calcium transporter a (cata) (formerly the testis-enhanced gene transfer (tegt) family"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HHT6"/>
    <dbReference type="GeneID" id="3077437"/>
    <dbReference type="KEGG" id="vg:3077437"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="PTHR23291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418323" description="Membrane protein US21">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="243" mass="26927" checksum="756CF7CE0E2B7334" modified="2011-06-28" version="1">MSLRGQVQIARSVFLLRIYILIWVQCLILMSVCAFCWLVLPHRLEQLFSSVRLTLSCLMISIVCLGLLRWAEPNFPKNVWILLTYTLLTSVAVTASGFHFSHRSVIYAMVATVTLFCFLTLATYLFARDVELQRSLLTGASTLILLLFAVFSLFPEAVSEILVMIAGLAVIVTSVVCDTQDILHDIEYESYIPGALCLYMDLMYLFVSVLYFMPSEPGSAHTAQTTVAATVAATAAASPQFVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-09-29" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5HI32</accession>
    <name>IR10_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein IRL10</fullName>
            <shortName>TRL10</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RL10</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HHV-5 RL10 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081458.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HI32"/>
    <dbReference type="PRIDE" id="F5HI32"/>
    <dbReference type="DNASU" id="3077518"/>
    <dbReference type="GeneID" id="3077518"/>
    <dbReference type="KEGG" id="vg:3077518"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009284">
        <property type="entry name" value="Cytomega_TRL10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06084">
        <property type="entry name" value="Cytomega_TRL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018454">
        <property type="entry name" value="Cytomega_TRL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418309" description="Protein IRL10">
        <location>
            <begin position="25"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="132"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="170" mass="18975" checksum="7C850AB8EF36E61C" modified="2011-06-28" version="1" precursor="true">MYPRVMHAVCFLALGLISYVAVCAENTVTTNCLVKAENTHLTCKCNPNTTSNTNNGSKCHAMCKCRVTEPITMLGAYSAWGAGSFVATLIVLLVVFFVIYAREEEKNNTGTEVDQCLAYRSLTRKKLEQHAAKKQNIYERIPYRPSRQNDNSPLIEPTGTDDEEDEDDDV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2021-06-02" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>F5HI68</accession>
    <name>UL19_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL19</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HHV-5 UL19 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081478.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HI68"/>
    <dbReference type="DNASU" id="3077478"/>
    <dbReference type="GeneID" id="3077478"/>
    <dbReference type="KEGG" id="vg:3077478"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020504">
        <property type="entry name" value="DUF5500"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17604">
        <property type="entry name" value="DUF5500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417844" description="Uncharacterized protein UL19">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="98" mass="11379" checksum="7E8A6875611E3F2B" modified="2011-06-28" version="1">MTSNALYELFRRRLPRAPVNTVMFLTRRTRDGFCGRLTSIATNSHYTMFVLDHGSVRIERPSQSEVDCASLMETLKRIRLRNSWVASEDELDVSRRDA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F5HIG1</accession>
    <name>HEPA_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA helicase/primase complex-associated protein</fullName>
            <shortName evidence="1">HEPA</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Primase-associated factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">UL102</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase presumably elongates using dNTPs. The primase-associated factor has no known catalytic activity in the complex and may serve to facilitate the formation of the replisome by directly interacting with the origin-binding protein and the polymerase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with the primase and the helicase to form the helicase-primase complex. Interacts with the origin-binding protein. Interacts with the polymerase catalytic subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae HEPA family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081548.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HIG1"/>
    <dbReference type="GeneID" id="3077548"/>
    <dbReference type="KEGG" id="vg:3077548"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04010">
        <property type="entry name" value="HSV_HEPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004996">
        <property type="entry name" value="HSV_HEPA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03324">
        <property type="entry name" value="Herpes_HEPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418266" description="DNA helicase/primase complex-associated protein">
        <location>
            <begin position="1"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="394"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="406"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="873" mass="94080" checksum="844F50617EBB045F" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5HIN9</accession>
    <name>TRX2_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Triplex capsid protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">TRX2</name>
        <name type="ordered locus">UL85</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Structural component of the T=16 icosahedral capsid. The capsid is composed of pentamers and hexamers of major capsid protein/MCP, which are linked together by heterotrimers called triplexes. These triplexes are formed by a single molecule of triplex protein 1/TRX1 and two copies of triplex protein 2/TRX2. Additionally, TRX1 is required for efficient transport of TRX2 to the nucleus, which is the site of capsid assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with TRX1 and major capisd protein/MCP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae TRX2 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081533.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HIN9"/>
    <dbReference type="BioGRID" id="1678069">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HIN9"/>
    <dbReference type="GeneID" id="3077516"/>
    <dbReference type="KEGG" id="vg:3077516"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04019">
        <property type="entry name" value="HSV_TRX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002690">
        <property type="entry name" value="Herpes_capsid_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01802">
        <property type="entry name" value="Herpes_V23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000418257" description="Triplex capsid protein 2">
        <location>
            <begin position="1"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04019"/>
        </source>
    </evidence>
    <sequence length="306" mass="34595" checksum="89A4E0FFE36B56C6" modified="2011-06-28" version="1">MAAMEANIFCTFDHKLSIADVGKLTKLVAAVVPIPQRLHLIKHYQLGLHQFVDHTRGYVRLRGLLRNMTLTLMRRVEGNQILLHVPTHGLLYTVLNTGPVTWEKGDALCVLPPLFHGPLARENLLTLGQWELVLPWIVPMPLALEINQRLLIMGLFSLDRSYEEVKAAVQQLQTITFRDATFTIPDPVIDQHLLIDMKTACLSMSMVANLASELTMTYVRKLALEDSSMLLVKCQELLMRLDRERSVGEPRTPARPQHVSPDDEIARLSALFVMLRQLDDLIREQVVFTVCDVSPDNKSATCIFKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F7V995</accession>
    <name>RL8A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein RL8A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RL8A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AEJ33666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_004940329.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F7V995"/>
    <dbReference type="GeneID" id="13229473"/>
    <dbReference type="KEGG" id="vg:13229469"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418307" description="Protein RL8A">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="90" mass="10140" checksum="B6D58E501B0A55BA" modified="2011-09-21" version="1">MPHGHLRQALSPTSWTCEGLLLLLGLLVLFFHHHNQSAVERRRRVSFVEADRLPHESGWYSSDDDGDRDGDEETGESHNRNSVGLSAVFS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>F7V999</accession>
    <name>US33A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein US33A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US33A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AEJ33670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_004940332.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F7V999"/>
    <dbReference type="GeneID" id="13229472"/>
    <dbReference type="KEGG" id="vg:13229472"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418326" description="Uncharacterized protein US33A">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <sequence length="57" mass="6914" checksum="6778139FCC4E67AC" modified="2011-09-21" version="1">MSLRFPERAGYEKLGYRPHAKRVWVHDPLGLTRFIMRQLMMYPLVLPFTFPFYVPRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-02-23" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q6RJQ3</accession>
    <accession>Q56JA4</accession>
    <accession>Q6E2A5</accession>
    <accession>Q6E2A6</accession>
    <accession>Q6E2A7</accession>
    <accession>Q6E2B3</accession>
    <accession>Q6E2B4</accession>
    <accession>Q6RJP5</accession>
    <accession>Q6RJP6</accession>
    <accession>Q6RJP7</accession>
    <accession>Q6RJP9</accession>
    <accession>Q6RJQ0</accession>
    <accession>Q6RJQ1</accession>
    <accession>Q6SWK7</accession>
    <name>UL141_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL141</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL141</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Arch. Virol." volume="151" first="827" last="835">
            <title>Sequence variability of the human cytomegalovirus UL141 open reading frame in clinical strains.</title>
            <authorList>
                <person name="Ma Y.P."/>
                <person name="Ruan Q."/>
                <person name="He R."/>
                <person name="Qi Y."/>
                <person name="Sun Z.R."/>
                <person name="Ji Y.H."/>
                <person name="Huang Y.J."/>
                <person name="Liu Q."/>
                <person name="Chen S.R."/>
                <person name="Wang J.D."/>
            </authorList>
            <dbReference type="PubMed" id="16195788"/>
            <dbReference type="DOI" id="10.1007/s00705-005-0638-2"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>Isolate 10J</strain>
            <strain>Isolate 12M</strain>
            <strain>Isolate 13J</strain>
            <strain>Isolate 14J</strain>
            <strain>Isolate 16m</strain>
            <strain>Isolate 18M</strain>
            <strain>Isolate 1M</strain>
            <strain>Isolate 20M</strain>
            <strain>Isolate 27C</strain>
            <strain>Isolate 29C</strain>
            <strain>Isolate 2J</strain>
            <strain>Isolate 32C</strain>
            <strain>Isolate 33J</strain>
            <strain>Isolate 39J</strain>
            <strain>Isolate 51C</strain>
            <strain>Isolate 63J</strain>
            <strain>Isolate 84</strain>
            <strain>Isolate 8J</strain>
            <strain>Isolate 9J</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="Nat. Immunol." volume="6" first="181" last="188">
            <title>Downregulation of natural killer cell-activating ligand CD155 by human cytomegalovirus UL141.</title>
            <authorList>
                <person name="Tomasec P."/>
                <person name="Wang E.C."/>
                <person name="Davison A.J."/>
                <person name="Vojtesek B."/>
                <person name="Armstrong M."/>
                <person name="Griffin C."/>
                <person name="McSharry B.P."/>
                <person name="Morris R.J."/>
                <person name="Llewellyn-Lacey S."/>
                <person name="Rickards C."/>
                <person name="Nomoto A."/>
                <person name="Sinzger C."/>
                <person name="Wilkinson G.W."/>
            </authorList>
            <dbReference type="PubMed" id="15640804"/>
            <dbReference type="DOI" id="10.1038/ni1156"/>
        </citation>
        <scope>INTERACTION WITH HUMAN PVR</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2010" name="J. Gen. Virol." volume="91" first="2034" last="2039">
            <title>Human cytomegalovirus UL141 promotes efficient downregulation of the natural killer cell activating ligand CD112.</title>
            <authorList>
                <person name="Prod'homme V."/>
                <person name="Sugrue D.M."/>
                <person name="Stanton R.J."/>
                <person name="Nomoto A."/>
                <person name="Davies J."/>
                <person name="Rickards C.R."/>
                <person name="Cochrane D."/>
                <person name="Moore M."/>
                <person name="Wilkinson G.W."/>
                <person name="Tomasec P."/>
            </authorList>
            <dbReference type="PubMed" id="20410314"/>
            <dbReference type="DOI" id="10.1099/vir.0.021931-0"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2013" name="Cell Host Microbe" volume="13" first="324" last="335">
            <title>Human cytomegalovirus glycoprotein UL141 targets the TRAIL death receptors to thwart host innate antiviral defenses.</title>
            <authorList>
                <person name="Smith W."/>
                <person name="Tomasec P."/>
                <person name="Aicheler R."/>
                <person name="Loewendorf A."/>
                <person name="Nemcovicova I."/>
                <person name="Wang E.C."/>
                <person name="Stanton R.J."/>
                <person name="Macauley M."/>
                <person name="Norris P."/>
                <person name="Willen L."/>
                <person name="Ruckova E."/>
                <person name="Nomoto A."/>
                <person name="Schneider P."/>
                <person name="Hahn G."/>
                <person name="Zajonc D.M."/>
                <person name="Ware C.F."/>
                <person name="Wilkinson G.W."/>
                <person name="Benedict C.A."/>
            </authorList>
            <dbReference type="PubMed" id="23498957"/>
            <dbReference type="DOI" id="10.1016/j.chom.2013.02.003"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HUMAN TNFRSF10A AND TNFRSF10B</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2013" name="PLoS Pathog." volume="9" first="E1003224" last="E1003224">
            <title>Structure of human cytomegalovirus UL141 binding to TRAIL-R2 reveals novel, non-canonical death receptor interactions.</title>
            <authorList>
                <person name="Nemcovicova I."/>
                <person name="Benedict C.A."/>
                <person name="Zajonc D.M."/>
            </authorList>
            <dbReference type="PubMed" id="23555243"/>
            <dbReference type="DOI" id="10.1371/journal.ppat.1003224"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 32-246</scope>
        <scope>INTERACTION WITH HOST TNFRSF10B</scope>
        <scope>GLYCOSYLATION AT ASN-117; ASN-132 AND ASN-147</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4">Evasion of NK cell killing. Blocks surface expression of PVR which is a ligand for NK cell-activating receptors. Binds human PVR in the endoplasmic reticulum and prevents its maturation and transport to the cell surface. Targets also the natural killer cell activating ligand NECTIN2 for proteasome-mediated degradation. Additionally promotes intracellular retention of TNFRSF10A/TRAIL-R1 and TNFRSF10B/TRAIL-R2 and thus down-regulates their cell surface expression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4 5">Interacts with human PVR. Interacts with human TNFRSF10A and TNFRSF10B. Forms a homodimer that engages two TNFRSF10B monomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Host endoplasmic reticulum membrane</location>
            <topology>Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text>Early-late protein.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The UL/b' region coding for this gene is deleted in some HHV-5 laboratory strains, like strains AD169 or Towne.</text>
    </comment>
    <dbReference type="EMBL" id="JX512197">
        <property type="protein sequence ID" value="AAR31489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY600463">
        <property type="protein sequence ID" value="AAT68302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY496555">
        <property type="protein sequence ID" value="AAR92108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY496547">
        <property type="protein sequence ID" value="AAR92100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY600467">
        <property type="protein sequence ID" value="AAT68306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY496549">
        <property type="protein sequence ID" value="AAR92102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY496550">
        <property type="protein sequence ID" value="AAR92103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY941105">
        <property type="protein sequence ID" value="AAX57274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY496551">
        <property type="protein sequence ID" value="AAR92104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY600465">
        <property type="protein sequence ID" value="AAT68304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY600466">
        <property type="protein sequence ID" value="AAT68305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY600464">
        <property type="protein sequence ID" value="AAT68303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY600462">
        <property type="protein sequence ID" value="AAT68301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY496552">
        <property type="protein sequence ID" value="AAR92105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY496548">
        <property type="protein sequence ID" value="AAR92101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY600468">
        <property type="protein sequence ID" value="AAT68307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY600461">
        <property type="protein sequence ID" value="AAT68300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY600459">
        <property type="protein sequence ID" value="AAT68298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY941104">
        <property type="protein sequence ID" value="AAX57273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY496554">
        <property type="protein sequence ID" value="AAR92107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY600460">
        <property type="protein sequence ID" value="AAT68299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY496553">
        <property type="protein sequence ID" value="AAR92106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081575.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PDB" id="4I9X">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B=32-246"/>
    </dbReference>
    <dbReference type="PDB" id="4JM0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.25 A"/>
        <property type="chains" value="A/B=30-279"/>
    </dbReference>
    <dbReference type="PDBsum" id="4I9X"/>
    <dbReference type="PDBsum" id="4JM0"/>
    <dbReference type="SMR" id="Q6RJQ3"/>
    <dbReference type="iPTMnet" id="Q6RJQ3"/>
    <dbReference type="PRIDE" id="Q6RJQ3"/>
    <dbReference type="DNASU" id="3077418"/>
    <dbReference type="GeneID" id="3077418"/>
    <dbReference type="KEGG" id="vg:3077418"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000169440">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031918">
        <property type="entry name" value="UL141"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038504">
        <property type="entry name" value="UL141-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16758">
        <property type="entry name" value="UL141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="SSF48726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1131">Modulation of host NK-cell activity by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000253802" description="Protein UL141">
        <location>
            <begin position="37"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="37"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="300"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1 5">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1 5">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1 5">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 1M.">
        <original>R</original>
        <variation>Q</variation>
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 45J.">
        <original>V</original>
        <variation>T</variation>
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 1M, Isolate 10J, Isolate 2J, Isolate 29C, Isolate 27C, Isolate 18M, Isolate 33J, Isolate 14, Isolate 32C, Isolate 39J, Isolate 63J, Isolate 25J and Isolate 9J.">
        <original>L</original>
        <variation>F</variation>
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 10J, Isolate 2J, Isolate 29C, Isolate 27C, Isolate 18M, Isolate 33J, Isolate 14, Isolate 32C, Isolate 39J and Isolate 63J.">
        <original>E</original>
        <variation>K</variation>
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 8J, Isolate 16m and Isolate 63J.">
        <original>Y</original>
        <variation>H</variation>
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 84.">
        <original>S</original>
        <variation>F</variation>
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 8J and Isolate 16m.">
        <original>S</original>
        <variation>L</variation>
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 45J.">
        <original>T</original>
        <variation>I</variation>
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 2J, Isolate 10J, Isolate 29C, Isolate 27C, Isolate 18M, Isolate 33J, Isolate 45J and Isolate 14.">
        <original>T</original>
        <variation>M</variation>
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 10J.">
        <original>G</original>
        <variation>S</variation>
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 2J, Isolate 10J, Isolate 25J, Isolate 39J, Isolate 45J, Isolate 29C, Isolate 27C, Isolate 18M, Isolate 33 and Isolate 14.">
        <original>M</original>
        <variation>T</variation>
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 2J, Isolate 10J, Isolate 25J, Isolate 29C, Isolate 27C, Isolate 39J, Isolate 18M, Isolate 33J and Isolate 14.">
        <original>A</original>
        <variation>V</variation>
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="sequence variant">
        <original>R</original>
        <variation>H</variation>
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 13J and Isolate 51C.">
        <original>R</original>
        <variation>C</variation>
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: Isolate 2J, Isolate 10J, Isolate 45J, Isolate 29C, Isolate 27C, Isolate 18M, Isolate 33J and Isolate 14.">
        <original>K</original>
        <variation>R</variation>
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="39"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="47"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="91"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="102"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="106"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="115"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="135"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="139"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="183"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="192"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="195"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="209"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="235"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15640804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="20410314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="23498957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="23555243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="4I9X"/>
        </source>
    </evidence>
    <sequence length="338" mass="38918" checksum="D3E1152EB676F6CE" modified="2004-07-05" version="1" precursor="true">MCRRESLRTLPWLFWVLLSCPRLLEYSSSSFPFATADIAEKMWAENYETTSPAPVLVAEGEQVTIPCTVMTHSWPMVSIRARFCRSHDGSDELILDAVKGHRLMNGLQYRLPYATWNFSQLHLGQIFSLTFNVSTDTAGMYECVLRNYSHGLIMQRFVILTQLETLSRPDEPCCTPALGRYSLGDQIWSPTPWRLRNHDCGMYRGFQRNYFYIGRADAEDCWKPACPDEEPDRCWTVIQRYRLPGDCYRSQPHPPKFLPVTPAPPADIDTGMSPWATRGIAAFLGFWSIFTVCFLCYLCYLQCCGRWCPTPGRGRRGGEGYRRLPTYDSYPGVKKMKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SVX2</accession>
    <accession>D2K3Y4</accession>
    <name>TRS1_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein TRS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">TRS1</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Inhibits the establishment of the antiviral state in the infected cell. Prevents the phosphorylation of the host eukaryotic translation initiation factor eIF-2alpha/EIF2S1 and thus the shutoff of viral and cellular protein synthesis by directly interacting with EIF2AK2/PKR. Also inhibits host autophagy by interacting with host Beclin-1/BECN1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host EIF2AK2/PKR; this interaction retains EIF2AK2 to the host nucleus and prevents its activation. Interaction (via N-terminus) with host BECN1; this interaction inhibits host autophagy. Interacts with the viral DNA polymerase accessory subunit UL44. Interacts with host HSPA5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae US22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081619.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BioGRID" id="1678076">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SVX2"/>
    <dbReference type="GeneID" id="3077523"/>
    <dbReference type="KEGG" id="vg:3077523"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030430">
        <property type="term" value="C:host cell cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030291">
        <property type="term" value="F:protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039521">
        <property type="term" value="P:suppression by virus of host autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039580">
        <property type="term" value="P:suppression by virus of host PKR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039502">
        <property type="term" value="P:suppression by virus of host type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1083">Inhibition of host autophagy by virus</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-1114">Inhibition of host interferon signaling pathway by virus</keyword>
    <keyword id="KW-1102">Inhibition of host PKR by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000418273" description="Protein TRS1">
        <location>
            <begin position="1"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="610"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="610"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="649"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="788" mass="83959" checksum="79DACA06405652E6" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SVZ5</accession>
    <accession>D2K3V6</accession>
    <name>US11_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane glycoprotein US11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US11</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Participates in the inhibition of the host immune response. Redirects newly synthesized major histocompatibility complex (MHC) class I heavy chains via the SEC61 translocon to the cytosol where they undergo proteasome-dependent destruction. In consequence, infected cells are masked for immune recognition by cytotoxic T-lymphocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with host TRAM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="developmental stage">
        <text>Expressed at early and late period of virus infection.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">N-glycosylated.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">A fraction of newly synthesized molecules retain the signal peptide after the N-linked glycan has been attached and translation of the polypeptide has been completed. Delayed cleavage of the signal peptide is determined by the first four residues, as well as by the transmembrane region.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytomegalovirus US6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081596.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BioGRID" id="1678043">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SVZ5"/>
    <dbReference type="DNASU" id="3077490"/>
    <dbReference type="GeneID" id="3077490"/>
    <dbReference type="KEGG" id="vg:3077490"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012536">
        <property type="entry name" value="CMV_US"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08001">
        <property type="entry name" value="CMV_US"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0393">Immunoglobulin domain</keyword>
    <keyword id="KW-1080">Inhibition of host adaptive immune response by virus</keyword>
    <keyword id="KW-1115">Inhibition of host MHC class I molecule presentation by virus</keyword>
    <keyword id="KW-0426">Late protein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418320" description="Membrane glycoprotein US11">
        <location>
            <begin position="18"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="3">
        <location>
            <begin position="18"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="3">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like H-type">
        <location>
            <begin position="47"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="56"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P09727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="215" mass="25288" checksum="6872C1A2B7C1D21F" modified="2004-07-05" version="1" precursor="true">MNLVMLILALWAPVAGSMPELSLTLFDEPPPLVETEPLPPLPDVSEYRVEYSEARCVLRSGGRLEALWTLRGNLSVPTPTPRVYYQTLEGYADRVPTPVEDISESLVAKRYWLRDYRVPQRTKLVLFYFSPCHQCQTYYVECEPRCLVPWVPLWSSLEDIERLLFEDRRLMAYYALTIKSAQYTLMMVAVIQVFWGLYVKGWLHRHFPWMFSDQW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW00</accession>
    <accession>D2K3V1</accession>
    <name>US06_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Unique short US6 glycoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US6</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Inhibits peptide loading of MHC class I molecules by transporters associated with antigen processing (TAP). Does not prevent peptide binding to TAP, but binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1, but not TAP2. Also prevents the conformational rearrangement of TAP induced by peptide binding. In consequence, infected cells are masked for immune recognition by cytotoxic T-lymphocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with UL18.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Host endoplasmic reticulum membrane</location>
            <topology>Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="developmental stage">
        <text>Expressed at early period of virus infection.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The ER-lumenal domain is responsible for TAP inhibition. It is sufficient to inhibit ATP binding to TAP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytomegalovirus US6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081591.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW00"/>
    <dbReference type="DNASU" id="3077555"/>
    <dbReference type="GeneID" id="3077555"/>
    <dbReference type="KEGG" id="vg:3077555"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035129">
        <property type="entry name" value="US6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17616">
        <property type="entry name" value="US6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0393">Immunoglobulin domain</keyword>
    <keyword id="KW-1080">Inhibition of host adaptive immune response by virus</keyword>
    <keyword id="KW-1107">Inhibition of host TAP by virus</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000417852" description="Unique short US6 glycoprotein">
        <location>
            <begin position="20"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like H-type">
        <location>
            <begin position="30"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="38"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="182" mass="20511" checksum="4D0567C4F8C74AFB" modified="2004-07-05" version="1" precursor="true">MDLLIRLGFLLMCALPTPGERSSRDPKTLLSLSPRQACVPRTKSHRPVCYNDTGDCTDADDSWKQLGEDFAHQCLQAAKKRPKTHKSRPNDRNLEGRLTCQRVRRLLPCDLDIHPSHRLLTLMNNCVCDGAVWNAFRLIERHGFFAVTLYLCCGITLLVVILALLCSITYESTGRGIRRCGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW03</accession>
    <accession>D2K3U8</accession>
    <name>US01_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein US1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US1</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081588.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW03"/>
    <dbReference type="GeneID" id="3077491"/>
    <dbReference type="KEGG" id="vg:3077491"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418291" description="Protein US1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="156" mass="17765" checksum="1AFD9457E2CC64E7" modified="2004-07-05" version="1">MASGLGDLSVGVSSLPMRELAWRRVADDSHDLWCCCMDWKAHVEYAHPASELRPGSGGWPEHAEAQWRQQVHAAHDVWCNCGDWQGHALRSRSRTAESGRSSSSSSVSVLSDGDQQPWWRRLRVKRPKFPSWARRWTQRHDSEERASQQAKNDSTS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW14</accession>
    <accession>D2K3T7</accession>
    <name>UL139_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane glycoprotein UL139</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL139</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081577.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="DNASU" id="3077570"/>
    <dbReference type="GeneID" id="3077570"/>
    <dbReference type="KEGG" id="vg:3077570"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021042">
        <property type="entry name" value="Herpes_UL139_cytomegalovirus"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12507">
        <property type="entry name" value="HCMV_UL139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418302" description="Membrane glycoprotein UL139">
        <location>
            <begin position="16"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="138" mass="14088" checksum="569C7706B7F3B3AF" modified="2004-07-05" version="1" precursor="true">MLWILVLFALAASASETTTGTSSNSSQSTSAGTTNTTTPSTACINASNGSDLGAPQLALLAASGWTLSGLLLIFTCCLCCFWLVRKVCSCCGNSSESESKATHAYTNAAFTSSDATLPMGTTGSYTPPQDGSFPPPPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW37</accession>
    <accession>D2K3R3</accession>
    <name>EP84_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Early phosphoprotein p84</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL112/UL113</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text>Needed for efficient replication. Recruits the DNA polymerase processivity factor to pre-replication foci.</text>
    </comment>
    <comment type="subunit">
        <text>Isoforms 1, 2, 3 and 4 interacts with themselves and with each other via their shared N-terminal regions; these interactions are important to both their intranuclear targeting and the recruitment of UL44 to subnuclear sites for viral replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
        <text evidence="1">Found in subnuclear structures known as promyelocytic leukemia oncogenic domains (PODs) or nuclear domain 10 (ND10) during the early.</text>
    </comment>
    <comment type="alternative products">
        <event type="alternative splicing"/>
        <isoform>
            <id>Q6SW37-1</id>
            <name>p84</name>
            <name>84 kDa phosphoprotein</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q6SW37-2</id>
            <name>p50</name>
            <name>50 kDa phosphoprotein</name>
            <sequence type="described" ref="VSP_042762 VSP_042765"/>
        </isoform>
        <isoform>
            <id>Q6SW37-3</id>
            <name>p43</name>
            <name>43 kDa phosphoprotein</name>
            <sequence type="described" ref="VSP_042763 VSP_042764"/>
        </isoform>
        <isoform>
            <id>Q6SW37-4</id>
            <name>p34</name>
            <name>34 kDa phosphoprotein</name>
            <sequence type="described" ref="VSP_042760 VSP_042761"/>
        </isoform>
        <text>Isoforms 1, 2, 3 and 4 share a common N-terminus.</text>
    </comment>
    <comment type="miscellaneous">
        <molecule>Isoform p43</molecule>
        <text evidence="4">Most abundant product which level of expression remains constant throughout infection.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the herpesviridae U79/UL112 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081553.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW37"/>
    <dbReference type="PRIDE" id="Q6SW37"/>
    <dbReference type="GeneID" id="3077495"/>
    <dbReference type="KEGG" id="vg:3077495"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004138">
        <property type="entry name" value="U79_P34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03064">
        <property type="entry name" value="U79_P34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0025">Alternative splicing</keyword>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000416710" description="Early phosphoprotein p84">
        <location>
            <begin position="1"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="166"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="357"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="407"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="569"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal" evidence="2">
        <location>
            <begin position="261"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="357"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_042760" description="In isoform p34." evidence="4">
        <original>SPPLREAKRQKTTAQ</original>
        <variation>ESRPSSRHGAFRVDS</variation>
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_042761" description="In isoform p34." evidence="4">
        <location>
            <begin position="269"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_042762" description="In isoform p50." evidence="4">
        <original>GALLLPIERGAVVSSPSSTSPSSLLSLPRPSSAHSAGETVQESEAAATAAAAGLMMMRRMRRAPAEAAEAPPQSEEEN</original>
        <variation>DSCQRRRAGLSSSTTAASRGPPQLSPQPALAPQQSHVRHSSPPVLYPQVPSPVSRPLPPQSKHQLLLPGPCVKSSPTW</variation>
        <location>
            <begin position="346"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_042763" description="In isoform p43." evidence="4">
        <original>ALLLPIERGAVV</original>
        <variation>IRGASASSQSAF</variation>
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_042764" description="In isoform p43." evidence="4">
        <location>
            <begin position="359"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_042765" description="In isoform p50." evidence="4">
        <location>
            <begin position="424"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P17151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="684" mass="70270" checksum="AF3B43A4D56B7E01" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW43</accession>
    <accession>D2K3Q7</accession>
    <name>GM_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Envelope glycoprotein M</fullName>
            <shortName evidence="1">gM</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gM</name>
        <name type="ORF">UL100</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Envelope glycoprotein important for virion assembly and egress. Plays a role in the correct incorporation of gH-gL into virion membrane. Directs the glycoprotein N (gN) to the host trans-Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts (via N-terminus) with gN (via N-terminus). The gM-gN heterodimer forms the gCII complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host Golgi apparatus</location>
            <location evidence="1">Host trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">During virion morphogenesis, this protein accumulates in the trans-Golgi network where secondary envelopment occurs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae glycoprotein M family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081547.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW43"/>
    <dbReference type="PRIDE" id="Q6SW43"/>
    <dbReference type="DNASU" id="3077534"/>
    <dbReference type="GeneID" id="3077534"/>
    <dbReference type="KEGG" id="vg:3077534"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04035">
        <property type="entry name" value="HSV_GM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000785">
        <property type="entry name" value="Herpes_glycop_M"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01528">
        <property type="entry name" value="Herpes_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00333">
        <property type="entry name" value="HSVINTEGRLMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1039">Host endosome</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000417830" description="Envelope glycoprotein M">
        <location>
            <begin position="1"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="1">
        <location>
            <begin position="35"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="1">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="1">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="1">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="1">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="1">
        <location>
            <begin position="261"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="1">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="1">
        <location>
            <begin position="320"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="346"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (with gN)" evidence="1">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="371" mass="42718" checksum="8EEA48CBB93F82F4" modified="2004-07-05" version="1">MAPSHVDKVNTRTWSASIVFMVLTFVNVSVHLVLSNFPHLGYPCVYYHVVDFERLNMSAYNVMHLHTPMLFLDSVQLVCYAVFMQLVFLAVTIYYLVCWIKISMRKDKGMSLNQSTRDISYMGDSLTAFLFILSMDTFQLFTLTMSFRLPSMIAFMAAVHFFCLTIFNVSMVTQYRSYKRSLFFFSRLHPKLKGTVQFRTLIVNLVEVALGFNTTVVAMALCYGFGNNFFVRTGHMVLAVFVVYAIISIIYFLLIEAVFFQYVKVQFGYHLGAFFGLCGLIYPIVQYDTFLSNEYRTGISWSFGMLFFIWAMFTTCRAVRYFRGRGSGSVKYQALATASGEEVAALSHHDSLESRRLREEEDDDDEDFEDA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW46</accession>
    <accession>D2K3Q4</accession>
    <name>UL97_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Serine/threonine protein kinase UL97</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL97</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="PLoS Pathog." volume="6" first="E1001092" last="E1001092">
            <title>Cyclin-dependent kinase-like function is shared by the beta- and gamma-subset of the conserved herpesvirus protein kinases.</title>
            <authorList>
                <person name="Kuny C.V."/>
                <person name="Chinchilla K."/>
                <person name="Culbertson M.R."/>
                <person name="Kalejta R.F."/>
            </authorList>
            <dbReference type="PubMed" id="20838604"/>
            <dbReference type="DOI" id="10.1371/journal.ppat.1001092"/>
        </citation>
        <scope>REVIEW ON FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Serine/threonine protein kinase that plays important roles in several processes including nuclear viral egress, viral replication or regulation of host cell cycle progression. Participates in the acquisition of tegument during virion morphogenesis in the nucleus. Redistributes the host nuclear lamina by phosphorylating cellular Lamins-A/C. Plays a role in viral DNA synthesis by phosphorylating the DNA polymerase processivity factor UL44. Stimulates host cell cycle to support viral DNA synthesis by phosphorylating host retinoblastoma/RB1 protein. Additional substrates have been identified including host EF1D or H2B. Phosphorylates also host SAMHD1 and thereby counteracts its antiviral effect by reducing its dNTP hydrolase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with UL83.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Autophosphorylates on serine and threonine residues.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Tyr protein kinase family. HCMV ganciclovir subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081544.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BioGRID" id="1678070">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW46"/>
    <dbReference type="GeneID" id="3077517"/>
    <dbReference type="KEGG" id="vg:3077517"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060153">
        <property type="term" value="P:modulation by virus of host cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010615">
        <property type="entry name" value="Herpes_UL97"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06734">
        <property type="entry name" value="UL97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-1121">Modulation of host cell cycle by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000416707" description="Serine/threonine protein kinase UL97">
        <location>
            <begin position="1"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="245"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P16788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="707" mass="78265" checksum="6E036BF7F7A27094" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW48</accession>
    <accession>D2K3Q2</accession>
    <name>UL95_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL95</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL95</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text>Participates in the expression of late viral mRNAs. Expressed before viral DNA replication, UL95 assembles at the viral pre-replication complexes (pre-RCs) containing the early viral protein UL44.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Host nucleus</location>
        </subcellularLocation>
        <text>Recruited to nuclear replication compartments.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae UL95 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081542.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW48"/>
    <dbReference type="GeneID" id="3077502"/>
    <dbReference type="KEGG" id="vg:3077502"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004280">
        <property type="entry name" value="Herpes_UL95"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03038">
        <property type="entry name" value="Herpes_UL95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417851" description="Protein UL95">
        <location>
            <begin position="1"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="533" mass="57273" checksum="C0B150143432FD4E" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW55</accession>
    <accession>D2K3P5</accession>
    <name>UL87_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL87</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL87</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Functions concordantly with UL87 to initiate transcription from over half of all active viral promoters in late infection, without affecting host transcription. Acts on and binds to viral early-late and late kinetic-class promoters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae UL87 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081535.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BioGRID" id="1678018">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW55"/>
    <dbReference type="GeneID" id="3077465"/>
    <dbReference type="KEGG" id="vg:3077465"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004285">
        <property type="entry name" value="Herpes_UL87_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007618">
        <property type="entry name" value="Herpes_UL87_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04532">
        <property type="entry name" value="DUF587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03043">
        <property type="entry name" value="Herpes_UL87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418288" description="Uncharacterized protein UL87">
        <location>
            <begin position="1"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="910"/>
            <end position="941"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="941" mass="104949" checksum="87A544B382475D25" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW62</accession>
    <accession>D2K3N8</accession>
    <accession>D2K3N9</accession>
    <accession>Q6SW61</accession>
    <name>SCAF_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Capsid scaffolding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Capsid protein P40</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Protease precursor</fullName>
            <shortName evidence="2">pPR</shortName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName evidence="2">Assemblin</fullName>
                <ecNumber evidence="2">3.4.21.97</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2">Protease</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="2">Assembly protein</fullName>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2">Capsid assembly protein</fullName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name type="primary">UL80</name>
        <name type="synonym">APNG</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <molecule>Capsid scaffolding protein</molecule>
        <text evidence="2">Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein. Cleavages products are evicted from the capsid before or during DNA packaging.</text>
    </comment>
    <comment type="function">
        <molecule>Assemblin</molecule>
        <text evidence="2">Protease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-Ser-, -Ala-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging.</text>
    </comment>
    <comment type="function">
        <molecule>Assembly protein</molecule>
        <text evidence="2">Plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.</text>
            <dbReference type="EC" id="3.4.21.97"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Capsid scaffolding protein homomultimerizes and interacts with major capsid protein. Assemblin exists in a monomer-dimer equilibrium with the dimer being the active species. Assembly protein homomultimerizes and interacts with major capsid protein.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Capsid scaffolding protein</molecule>
        <subcellularLocation>
            <location evidence="2">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Assemblin</molecule>
        <subcellularLocation>
            <location evidence="2">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Assembly protein</molecule>
        <subcellularLocation>
            <location evidence="2">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="alternative promoter"/>
        <isoform>
            <id>Q6SW62-1</id>
            <name>Capsid scaffolding protein</name>
            <name>pPR</name>
            <name>UL80a</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q6SW62-2</id>
            <name>pAP</name>
            <name>Assembly protein</name>
            <name>UL80.5</name>
            <sequence type="described" ref="VSP_044019"/>
        </isoform>
        <isoform>
            <id>Q6SW62-3</id>
            <name>UL80.4 protein</name>
            <sequence type="described" ref="VSP_044018"/>
        </isoform>
        <isoform>
            <id>Q6SW62-4</id>
            <name>UL80.3 protein</name>
            <sequence type="described" ref="VSP_044017"/>
        </isoform>
    </comment>
    <comment type="domain">
        <text evidence="2">Region of interaction between pPR and pAP is called Amino conserved domain (ACD). The region of interaction with major capsid protein is called carboxyl conserved domain (CCD).</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">Capsid scaffolding protein is cleaved by assemblin after formation of the spherical procapsid. As a result, the capsid obtains its mature, icosahedral shape. Cleavages occur at two or more sites: release and tail site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae capsid scaffolding protein family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAR31633" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.4.21.97" evidence="2"/>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31633.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081528.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081529.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW62"/>
    <dbReference type="MEROPS" id="S21.002"/>
    <dbReference type="PRIDE" id="Q6SW62"/>
    <dbReference type="DNASU" id="3077443"/>
    <dbReference type="GeneID" id="3077443"/>
    <dbReference type="GeneID" id="3077485"/>
    <dbReference type="KEGG" id="vg:3077443"/>
    <dbReference type="KEGG" id="vg:3077485"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030430">
        <property type="term" value="C:host cell cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019028">
        <property type="term" value="C:viral capsid"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039708">
        <property type="term" value="P:nuclear capsid assembly"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019076">
        <property type="term" value="P:viral release from host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.16.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04008">
        <property type="entry name" value="HSV_SCAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035443">
        <property type="entry name" value="Herpes_virus_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001847">
        <property type="entry name" value="Peptidase_S21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00716">
        <property type="entry name" value="Peptidase_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00236">
        <property type="entry name" value="HSVCAPSIDP40"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0877">Alternative promoter usage</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <keyword id="KW-0118">Viral capsid assembly</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <feature type="chain" id="PRO_0000418270" description="Capsid scaffolding protein">
        <location>
            <begin position="1"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418271" description="Assemblin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418272" description="Assembly protein" evidence="2">
        <location>
            <begin position="257"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="270"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with pAP" evidence="2">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="455"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="593"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with major capsid protein" evidence="2">
        <location>
            <begin position="688"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal 1" evidence="1">
        <location>
            <begin position="510"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal 2" evidence="1">
        <location>
            <begin position="537"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="294"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="455"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="551"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by assemblin; Release site" evidence="2">
        <location>
            <begin position="256"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by assemblin; Maturation site">
        <location>
            <begin position="643"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by assemblin; Tail site">
        <location>
            <begin position="684"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_044017" description="In isoform UL80.3 protein." evidence="4">
        <location>
            <begin position="1"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_044018" description="In isoform UL80.4 protein." evidence="4">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_044019" description="In isoform pAP." evidence="4">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="708" mass="73838" checksum="28106DF482DB0521" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW65</accession>
    <accession>D2K3N5</accession>
    <name>CVC2_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Capsid vertex component 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">CVC2</name>
        <name type="ordered locus">UL77</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Capsid vertex-specific component that plays a role during viral DNA encapsidation, assuring correct genome cleavage and presumably stabilizing capsids that contain full-length viral genomes. Participates in the interaction between the capsid and the tegument through interaction with the large tegument protein/LTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimerizes with CVC1. Interacts with major capsid protein/MCP and triplex capsid protein 1/TRX1 at the pentamer vertices. Interacts with the large tegument protein/LTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae CVC2 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081525.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW65"/>
    <dbReference type="BioGRID" id="1678004">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW65"/>
    <dbReference type="GeneID" id="3077451"/>
    <dbReference type="KEGG" id="vg:3077451"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04025">
        <property type="entry name" value="HSV_CVC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002493">
        <property type="entry name" value="Herpes_UL25"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01499">
        <property type="entry name" value="Herpes_UL25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0231">Viral genome packaging</keyword>
    <keyword id="KW-1163">Viral penetration into host nucleus</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="chain" id="PRO_0000418317" description="Capsid vertex component 2">
        <location>
            <begin position="1"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with major capsid protein/MCP" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="97"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="636" mass="70644" checksum="D2C0A57D85F33CC4" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW67</accession>
    <accession>D2K3N3</accession>
    <name>GH_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Envelope glycoprotein H</fullName>
            <shortName evidence="1">gH</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gH</name>
        <name type="synonym">UL75</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="J. Virol." volume="82" first="60" last="70">
            <title>Characterization of the human cytomegalovirus gH/gL/UL128-131 complex that mediates entry into epithelial and endothelial cells.</title>
            <authorList>
                <person name="Ryckman B.J."/>
                <person name="Rainish B.L."/>
                <person name="Chase M.C."/>
                <person name="Borton J.A."/>
                <person name="Nelson J.A."/>
                <person name="Jarvis M.A."/>
                <person name="Johnson D.C."/>
            </authorList>
            <dbReference type="PubMed" id="17942555"/>
            <dbReference type="DOI" id="10.1128/JVI.01910-07"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH GL; UL128; UL130 AND UL131A</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2013" name="PLoS Pathog." volume="9" first="E1003463" last="E1003463">
            <title>The HCMV gH/gL/UL128-131 complex triggers the specific cellular activation required for efficient viral internalization into target monocytes.</title>
            <authorList>
                <person name="Nogalski M.T."/>
                <person name="Chan G.C."/>
                <person name="Stevenson E.V."/>
                <person name="Collins-McMillen D.K."/>
                <person name="Yurochko A.D."/>
            </authorList>
            <dbReference type="PubMed" id="23853586"/>
            <dbReference type="DOI" id="10.1371/journal.ppat.1003463"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2013" name="J. Virol." volume="87" first="9680" last="9690">
            <title>Comparative analysis of gO isoforms reveals that strains of human cytomegalovirus differ in the ratio of gH/gL/gO and gH/gL/UL128-131 in the virion envelope.</title>
            <authorList>
                <person name="Zhou M."/>
                <person name="Yu Q."/>
                <person name="Wechsler A."/>
                <person name="Ryckman B.J."/>
            </authorList>
            <dbReference type="PubMed" id="23804643"/>
            <dbReference type="DOI" id="10.1128/JVI.01167-13"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2014" name="Acta Virol." volume="58" first="103" last="107">
            <title>Characteristics and functions of human cytomegalovirus UL128 gene/protein.</title>
            <authorList>
                <person name="Tao R."/>
                <person name="Xu J."/>
                <person name="Gao H."/>
                <person name="Zhao W."/>
                <person name="Shang S."/>
            </authorList>
            <dbReference type="PubMed" id="24957713"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2017" name="PLoS Pathog." volume="13" first="E1006281" last="E1006281">
            <title>Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-alpha as a key for entry.</title>
            <authorList>
                <person name="Wu Y."/>
                <person name="Prager A."/>
                <person name="Boos S."/>
                <person name="Resch M."/>
                <person name="Brizic I."/>
                <person name="Mach M."/>
                <person name="Wildner S."/>
                <person name="Scrivano L."/>
                <person name="Adler B."/>
            </authorList>
            <dbReference type="PubMed" id="28403202"/>
            <dbReference type="DOI" id="10.1371/journal.ppat.1006281"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH GO; GL AND HOST PDGFRA</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2016" name="J. Virol." volume="90" first="4926" last="4938">
            <title>The Human Cytomegalovirus UL116 Gene Encodes an Envelope Glycoprotein Forming a Complex with gH Independently from gL.</title>
            <authorList>
                <person name="Calo S."/>
                <person name="Cortese M."/>
                <person name="Ciferri C."/>
                <person name="Bruno L."/>
                <person name="Gerrein R."/>
                <person name="Benucci B."/>
                <person name="Monda G."/>
                <person name="Gentile M."/>
                <person name="Kessler T."/>
                <person name="Uematsu Y."/>
                <person name="Maione D."/>
                <person name="Lilja A.E."/>
                <person name="Carfi A."/>
                <person name="Merola M."/>
            </authorList>
            <dbReference type="PubMed" id="26937030"/>
            <dbReference type="DOI" id="10.1128/jvi.02517-15"/>
        </citation>
        <scope>INTERACTION WITH UL116</scope>
        <source>
            <strain>TR</strain>
        </source>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2018" name="Cell" volume="0" first="0" last="0">
            <title>An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.</title>
            <authorList>
                <person name="Martinez-Martin N."/>
                <person name="Marcandalli J."/>
                <person name="Huang C.S."/>
                <person name="Arthur C.P."/>
                <person name="Perotti M."/>
                <person name="Foglierini M."/>
                <person name="Ho H."/>
                <person name="Dosey A.M."/>
                <person name="Shriver S."/>
                <person name="Payandeh J."/>
                <person name="Leitner A."/>
                <person name="Lanzavecchia A."/>
                <person name="Perez L."/>
                <person name="Ciferri C."/>
            </authorList>
            <dbReference type="PubMed" id="30057110"/>
            <dbReference type="DOI" id="10.1016/j.cell.2018.06.028"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HOST NRP2</scope>
    </reference>
    <reference key="9">
        <citation type="journal article" date="2017" name="Sci. Immunol." volume="2" first="0" last="0">
            <title>Structural basis for potent antibody-mediated neutralization of human cytomegalovirus.</title>
            <authorList>
                <person name="Chandramouli S."/>
                <person name="Malito E."/>
                <person name="Nguyen T."/>
                <person name="Luisi K."/>
                <person name="Donnarumma D."/>
                <person name="Xing Y."/>
                <person name="Norais N."/>
                <person name="Yu D."/>
                <person name="Carfi A."/>
            </authorList>
            <dbReference type="PubMed" id="28783665"/>
            <dbReference type="DOI" id="10.1126/sciimmunol.aan1457"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.02 ANGSTROMS) OF 1-715</scope>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH GL; UL128; UL130 AND UL131A</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 4 6 7 8">The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding to host receptor, membrane fusion is mediated by the fusion machinery composed of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion morphogenesis (By similarity). In human cytomegalovirus, forms two distincts complexes to mediate viral entry, a trimer and a pentamer at the surface of the virion envelope. The gH-gL-gO trimer is required for infection in fibroblasts by interacting with host PDGFRA (PubMed:28403202). The gH-gL-UL128-UL130-UL131A pentamer is essential for viral entry in epithelial, endothelial and myeloid cells via interaction with host NRP2 (PubMed:17942555, PubMed:23853586, PubMed:30057110, PubMed:28783665).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 5 6 7 8">Interacts with glycoprotein L (gL); this interaction is necessary for the correct processing and cell surface expression of gH. The heterodimer gH/gL seems to interact with gB trimers during fusion (By similarity). Forms the envelope pentamer complex (PC) composed of gH, gL, UL128, UL130, and UL131A (PubMed:17942555, PubMed:28783665). The pentamer interacts with host NRP2 (PubMed:30057110). Forms the envelope trimer complex composed of gH, gL, and gO. The trimer interacts with host PDGFRA (PubMed:28403202). Interacts with UL116 (PubMed:26937030).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Virion membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host endosome membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN).</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">N-glycosylated, O-glycosylated, and sialylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae glycoprotein H family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081523.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PDB" id="5VOB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.02 A"/>
        <property type="chains" value="A=1-715"/>
    </dbReference>
    <dbReference type="PDB" id="5VOC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.99 A"/>
        <property type="chains" value="A=1-715"/>
    </dbReference>
    <dbReference type="PDB" id="5VOD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="5.90 A"/>
        <property type="chains" value="A=1-715"/>
    </dbReference>
    <dbReference type="PDB" id="7LBE">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A=1-715"/>
    </dbReference>
    <dbReference type="PDB" id="7LBF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A=1-715"/>
    </dbReference>
    <dbReference type="PDB" id="7LBG">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A=1-715"/>
    </dbReference>
    <dbReference type="PDBsum" id="5VOB"/>
    <dbReference type="PDBsum" id="5VOC"/>
    <dbReference type="PDBsum" id="5VOD"/>
    <dbReference type="PDBsum" id="7LBE"/>
    <dbReference type="PDBsum" id="7LBF"/>
    <dbReference type="PDBsum" id="7LBG"/>
    <dbReference type="SMR" id="Q6SW67"/>
    <dbReference type="TCDB" id="1.G.22.1.1">
        <property type="family name" value="the cytomegalovirus (human herpesvirus 5) glycoprotein go (go) family"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW67"/>
    <dbReference type="GeneID" id="3077450"/>
    <dbReference type="KEGG" id="vg:3077450"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044175">
        <property type="term" value="C:host cell endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019031">
        <property type="term" value="C:viral envelope"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055036">
        <property type="term" value="C:virion membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098670">
        <property type="term" value="P:entry receptor-mediated virion attachment to host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019064">
        <property type="term" value="P:fusion of virus membrane with host plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046718">
        <property type="term" value="P:viral entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04033">
        <property type="entry name" value="HSV_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003493">
        <property type="entry name" value="Herpes_gH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035305">
        <property type="entry name" value="Herpes_glycoH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038172">
        <property type="entry name" value="Herpes_glycoH_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17488">
        <property type="entry name" value="Herpes_glycoH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1169">Fusion of virus membrane with host cell membrane</keyword>
    <keyword id="KW-1168">Fusion of virus membrane with host membrane</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1039">Host endosome</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0730">Sialic acid</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-1161">Viral attachment to host cell</keyword>
    <keyword id="KW-1234">Viral attachment to host entry receptor</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-1162">Viral penetration into host cytoplasm</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
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</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW70</accession>
    <accession>D2K3M9</accession>
    <name>DUT_HCMVM</name>
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            <fullName evidence="1">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="1">dUTPase</shortName>
            <ecNumber evidence="1">3.6.1.23</ecNumber>
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    </protein>
    <gene>
        <name evidence="1" type="primary">DUT</name>
        <name type="ordered locus">UL72</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in nucleotide metabolism: produces dUMP, the immediate precursor of thymidine nucleotides and decreases the intracellular concentration of dUTP to avoid uracil incorporation into viral DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dUTP + H2O = diphosphate + dUMP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
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            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
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            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dUTPase family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="pathway name" value="HCMV Early Events"/>
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        <property type="reaction ID" value="UER00666"/>
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        <property type="component" value="Genome"/>
    </dbReference>
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        <property type="entry name" value="HSV_DUT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="dUTPase-like_sf"/>
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        <property type="entry name" value="Herpes_Orf11"/>
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        <property type="entry name" value="HSV_DUT"/>
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        <property type="entry name" value="SSF51283"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW73</accession>
    <accession>D2K3M6</accession>
    <name>ICP27_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>mRNA export factor ICP27 homolog</fullName>
        </recommendedName>
    </protein>
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        <name type="ORF">UL69</name>
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            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
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    </organism>
    <organismHost>
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        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
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        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
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                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="J. Gen. Virol." volume="88" first="405" last="410">
            <title>Multimerization of human cytomegalovirus regulatory protein UL69 via a domain that is conserved within its herpesvirus homologues.</title>
            <authorList>
                <person name="Lischka P."/>
                <person name="Thomas M."/>
                <person name="Toth Z."/>
                <person name="Mueller R."/>
                <person name="Stamminger T."/>
            </authorList>
            <dbReference type="PubMed" id="17251556"/>
            <dbReference type="DOI" id="10.1099/vir.0.82480-0"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>MULTIMERIZATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Immediate early (EI) protein that plays many roles during productive infection including regulation of host cell cycle progression, regulation of viral gene expression or nuclear export of intronless viral RNAs. Acts as a transcriptional transactivator via interaction with the cellular transcription elongation factor SUPT6H and as a nuclear RNA export factor via interaction with UAP56, a component of the cellular mRNA export machinery.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Self-associates and forms high-molecular-mass complexes. Interacts with host DDX39A and DDX39B; these interactions are required for UL69 function in mRNA export. Interacts with host SUPT6H, EIF4A1 AND PABPC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cytoplasm</location>
        </subcellularLocation>
        <text>Shuttles between host nucleus and cytoplasm.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated by UL97 and host CDK1, CDK7 and CD9. Phosphorylation by CDKs impacts on UL69 nuclear localization and activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HHV-1 ICP27 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081517.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW73"/>
    <dbReference type="GeneID" id="3077554"/>
    <dbReference type="KEGG" id="vg:3077554"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072517">
        <property type="term" value="C:host cell viral assembly compartment"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039645">
        <property type="term" value="P:modulation by virus of host G1/S transition checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008648">
        <property type="entry name" value="ICP27-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05459">
        <property type="entry name" value="Herpes_UL69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1078">G1/S host cell cycle checkpoint dysregulation by virus</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1121">Modulation of host cell cycle by virus</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000416711" description="mRNA export factor ICP27 homolog">
        <location>
            <begin position="1"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CHC2-type" evidence="2">
        <location>
            <begin position="387"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="540"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="574"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="688"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P10238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="17251556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="742" mass="82397" checksum="D6CF0E47B935A672" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW79</accession>
    <accession>D2K3M0</accession>
    <name>UL52_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Packaging protein UL32 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL52</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in efficient localization of neo-synthesized capsids to nuclear replication compartments, thereby controlling cleavage and packaging of virus genomic DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the herpesviridae UL32 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081511.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW79"/>
    <dbReference type="PRIDE" id="Q6SW79"/>
    <dbReference type="DNASU" id="3077432"/>
    <dbReference type="GeneID" id="3077432"/>
    <dbReference type="KEGG" id="vg:3077432"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030430">
        <property type="term" value="C:host cell cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019031">
        <property type="term" value="C:viral envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002597">
        <property type="entry name" value="Herpes_env"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01673">
        <property type="entry name" value="Herpes_env"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51988">
        <property type="entry name" value="HERPESVIRUS_UL32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000418287" description="Packaging protein UL32 homolog">
        <location>
            <begin position="1"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Zinc finger 1" evidence="2">
        <location>
            <begin position="200"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="392"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Zinc finger 2" evidence="2">
        <location>
            <begin position="459"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="567"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="574"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="668" mass="74119" checksum="FB12353FE11C0E31" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW81</accession>
    <accession>D2K3L8</accession>
    <name>NEC2_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nuclear egress protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NEC2</name>
        <name type="ordered locus">UL50</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2015" name="J. Biol. Chem." volume="290" first="27452" last="27458">
            <title>Crystal Structure of the Human Cytomegalovirus pUL50-pUL53 Core Nuclear Egress Complex Provides Insight into a Unique Assembly Scaffold for Virus-Host Protein Interactions.</title>
            <authorList>
                <person name="Walzer S.A."/>
                <person name="Egerer-Sieber C."/>
                <person name="Sticht H."/>
                <person name="Sevvana M."/>
                <person name="Hohl K."/>
                <person name="Milbradt J."/>
                <person name="Muller Y.A."/>
                <person name="Marschall M."/>
            </authorList>
            <dbReference type="PubMed" id="26432641"/>
            <dbReference type="DOI" id="10.1074/jbc.c115.686527"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.44 ANGSTROMS) OF 1-175</scope>
        <scope>INTERACTION WITH NEC1</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Plays an essential role in virion nuclear egress, the first step of virion release from infected cell. Within the host nucleus, NEC1 interacts with the newly formed capsid through the vertexes and directs it to the inner nuclear membrane by associating with NEC2. Induces the budding of the capsid at the inner nuclear membrane as well as its envelopment into the perinuclear space. There, the NEC1/NEC2 complex promotes the fusion of the enveloped capsid with the outer nuclear membrane and the subsequent release of the viral capsid into the cytoplasm where it will reach the secondary budding sites in the host Golgi or trans-Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Forms a heterohexameric complex with NEC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host nucleus inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="2">Localizes also at the transient membrane of perinuclear virions.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1 2">Phosphorylated (By similarity). Phosphorylation by viral kinase UL97 at Ser-216 plays an important role for correct viral nuclear egress complex (NEC) localization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae NEC2 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081509.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PDB" id="5D5N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.44 A"/>
        <property type="chains" value="A=1-175"/>
    </dbReference>
    <dbReference type="PDBsum" id="5D5N"/>
    <dbReference type="SMR" id="Q6SW81"/>
    <dbReference type="PRIDE" id="Q6SW81"/>
    <dbReference type="DNASU" id="3077439"/>
    <dbReference type="GeneID" id="3077439"/>
    <dbReference type="KEGG" id="vg:3077439"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04024">
        <property type="entry name" value="HSV_NEC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007626">
        <property type="entry name" value="Herpesvirus_viron_egress-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04541">
        <property type="entry name" value="Herpes_U34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0426">Late protein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000416719" description="Nuclear egress protein 2">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="topological domain" description="Perinuclear space" evidence="2">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="topological domain" description="Nuclear" evidence="2">
        <location>
            <begin position="383"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="202"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="306"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="5"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="23"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="26"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="31"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="42"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="71"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="81"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="111"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="116"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="130"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="139"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="144"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="26432641"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="5">
        <source>
            <dbReference type="PDB" id="5D5N"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="5D5N"/>
        </source>
    </evidence>
    <sequence length="398" mass="43052" checksum="367FF459968AD468" modified="2004-07-05" version="1">MEMNKVLHQDLVQATRRILKLGPSELRVTDAGLICKNPNYSVCDAMLKTDTVYCVEYLLSYWESRTDHVPCFIFKNTGCAVSLCCFVRAPVKLVSPARHVGEFNVLKVNESLIVTLKDIEEIKPSAYGVLTKCVVRKSNSASVFNIELIAFGPENEGEYENLLRELYAKKAASTSLAVRNHVTVSSHSGSGPSLWRARMSAALTRTAGKRSPRTASPPPPPPRHPSCSPTMVAAGGAAAGPRPPPPPMAAGSWRLCRCEACMGRCGCASEGDADEEEEELLALAGEGKAAAAAAGQDIGGSARRPLEEHVSRRRGVSTHHRHPPSPPCTPSLERTGYRWAPSSWWRARSGPSRPQSGPWLPARFATLGPLVLALLLVLALLWRGHGQSSSPTRSAHRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW82</accession>
    <accession>D2K3L7</accession>
    <name>UL49_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL49</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL49</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae UL49 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081508.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW82"/>
    <dbReference type="DNASU" id="3077510"/>
    <dbReference type="GeneID" id="3077510"/>
    <dbReference type="KEGG" id="vg:3077510"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004339">
        <property type="entry name" value="UL49"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03117">
        <property type="entry name" value="Herpes_UL49_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418248" description="Protein UL49">
        <location>
            <begin position="1"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="570" mass="63833" checksum="2BD8E44E353613E2" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW84</accession>
    <accession>D2K3L5</accession>
    <name>LTP_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large tegument protein deneddylase</fullName>
            <ecNumber evidence="1">3.4.19.12</ecNumber>
            <ecNumber evidence="1">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL48</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Large tegument protein that plays multiple roles in the viral cycle. During viral entry, remains associated with the capsid while most of the tegument is detached and participates in the capsid transport toward the host nucleus. Plays a role in the routing of the capsid at the nuclear pore complex and subsequent uncoating. Within the host nucleus, acts as a deneddylase and promotes the degradation of nuclear CRLs (cullin-RING ubiquitin ligases) and thereby stabilizes nuclear CRL substrates, while cytoplasmic CRLs remain unaffected. These modifications prevent host cell cycle S-phase progression and create a favorable environment allowing efficient viral genome replication. Participates later in the secondary envelopment of capsids. Indeed, plays a linker role for the association of the outer viral tegument to the capsids together with the inner tegument protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins. Interacts with inner tegument protein. Interacts with capsid vertex specific component CVC2. Interacts with the major capsid protein/MCP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <text evidence="1">Tightly associated with the capsid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae large tegument protein family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="1"/>
    <dbReference type="EC" id="3.4.22.-" evidence="1"/>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081506.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW84"/>
    <dbReference type="BioGRID" id="1678057">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW84"/>
    <dbReference type="DNASU" id="3077504"/>
    <dbReference type="GeneID" id="3077504"/>
    <dbReference type="KEGG" id="vg:3077504"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072517">
        <property type="term" value="C:host cell viral assembly compartment"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039648">
        <property type="term" value="P:modulation by virus of host protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04044">
        <property type="entry name" value="HSV_LTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006928">
        <property type="entry name" value="Herpes_teg_USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034702">
        <property type="entry name" value="HSV_LTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04843">
        <property type="entry name" value="Herpes_teg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51521">
        <property type="entry name" value="HTUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1127">Modulation of host ubiquitin pathway by viral deubiquitinase</keyword>
    <keyword id="KW-1130">Modulation of host ubiquitin pathway by virus</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0788">Thiol protease</keyword>
    <keyword id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000416709" description="Large tegument protein deneddylase">
        <location>
            <begin position="1"/>
            <end position="2241"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C76" evidence="1">
        <location>
            <begin position="4"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Deubiquitination activity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="239"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with inner tegument protein" evidence="1">
        <location>
            <begin position="327"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1187"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2118"/>
            <end position="2152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2131"/>
            <end position="2151"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="2241" mass="253174" checksum="83C76E6D72FA039B" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW87</accession>
    <accession>D2K3L2</accession>
    <name>RIR1_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonucleoside-diphosphate reductase large subunit-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">RIR1</name>
        <name type="synonym">UL45</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Does not possess a ribonucleotide reductase activity. Betaherpesviruses probably use another strategy to expand the dNTP pool in a quiescent host cell.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks the conserved sequence Asn-x-Cys-x-Glu essential for ribonucleotide reductase activity.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081503.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW87"/>
    <dbReference type="BioGRID" id="1677997">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW87"/>
    <dbReference type="DNASU" id="3077444"/>
    <dbReference type="GeneID" id="3077444"/>
    <dbReference type="KEGG" id="vg:3077444"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04027">
        <property type="entry name" value="HSV_RIR1_betahv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034716">
        <property type="entry name" value="HSV_RIR1_betahv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="PTHR11573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-0426">Late protein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000418312" description="Ribonucleoside-diphosphate reductase large subunit-like protein">
        <location>
            <begin position="1"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="906" mass="101698" checksum="B99A2500AB30F063" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW89</accession>
    <accession>D2K3L0</accession>
    <name>UL43_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Tegument protein UL43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL43</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae US22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081501.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW89"/>
    <dbReference type="DNASU" id="3077512"/>
    <dbReference type="GeneID" id="3077512"/>
    <dbReference type="KEGG" id="vg:3077512"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000416718" description="Tegument protein UL43">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="423" mass="47748" checksum="7D693A18C759408C" modified="2004-07-05" version="1">MEKTPAETTAVSAGNVPRDSIPCITNVSADTRGRTRPSRPATVPQRRPARIGHFRRRSASLSFLDWPDDSVTEGVRTTSASVAASAARFDEIRRRRQSINDEMKERTLEDALAVELVNETFRCSVTSDARKDLQKLVRRVSGTVLRLSWPNGWFFTYCDLLRVGYFGHLNIKGLEKTFLCCDKFLLPVGTVSRCEAIGRPPLPVLIGEGGRVYVYSPVVESLYLVSRSGFRGFVQEGLRNYAPLREELGYVRFETGGDVGREFMLARDLLALWRLCMKREGSIFSWRDGNEALTTVVLNGSQTYEDPAHGNWLKETCSLNVLQVFVVRAVPVESQQRLDISILVNESGAVFGVHPDTRQAHFLARGLLGFFRVGFLRFCNNYCFARDCFTHPESVAPAYRATGCPRELFCRRLRKKKGLFARR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW94</accession>
    <accession>D2K3K5</accession>
    <name>VGLI_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>UL37 immediate early glycoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL37</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Isoform vMIA sequesters proapoptotic BAX at the outer mitochondrial membrane and prevents cytochrome c release and subsequent initiation of the proapoptotic cascade. Also provoques a calcium efflux from host endoplasmic reticulum and F-actin cytoskeleton disruption. Participates in the increase of host mitochondrial biogenesis, thus promoting viral replication by efficient use of newly made mitochondria.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Isoform gpUL37 may play a role in escape from the host antiviral response.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Isoform vMIA interacts with host BAX. Isoform vMIA interacts with host RSAD2/viperin; this interaction results in RSAD2/viperin relocalization from the endoplasmic reticulum to the mitochondria, actin cytoskeleton disruption and enhancement of infection.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Isoform gpUL37</molecule>
        <subcellularLocation>
            <location evidence="1">Host membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">The C-terminal fragment localizes to the endoplasmic reticulum while the N-terminal fragment is stable and traffics to mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Isoform vMIA</molecule>
        <subcellularLocation>
            <location evidence="1">Host mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">Transported from the endoplasmic reticulum (ER) through the mitochondrial associated membrane (MAMs) to the mitochondrial outer membrane. Associates with internal lipid rafts (LRs) in the MAM (By similarity).</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Isoform pUL37m</molecule>
        <subcellularLocation>
            <location evidence="1">Host mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">Not cleaved or N-glycosylated.</text>
    </comment>
    <comment type="alternative products">
        <event type="alternative splicing"/>
        <isoform>
            <id>Q6SW94-1</id>
            <name>gpUL37</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q6SW94-2</id>
            <name>vMIA</name>
            <sequence type="described" ref="VSP_044022 VSP_044023"/>
        </isoform>
        <isoform>
            <id>Q6SW94-3</id>
            <name>pUL37m</name>
            <sequence type="described" ref="VSP_044024"/>
        </isoform>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the immediate early glycoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081496.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BMRB" id="Q6SW94"/>
    <dbReference type="PRIDE" id="Q6SW94"/>
    <dbReference type="GeneID" id="3077462"/>
    <dbReference type="KEGG" id="vg:3077462"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010880">
        <property type="entry name" value="Herpes_UL37_HHV-5-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07413">
        <property type="entry name" value="Herpes_UL37_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0025">Alternative splicing</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-1045">Host mitochondrion</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1119">Modulation of host cell apoptosis by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418298" description="UL37 immediate early glycoprotein">
        <location>
            <begin position="23"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="83"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage site" evidence="1">
        <location>
            <begin position="193"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_044022" description="In isoform vMIA." evidence="4">
        <original>H</original>
        <variation>Q</variation>
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_044023" description="In isoform vMIA." evidence="4">
        <location>
            <begin position="164"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_044024" description="In isoform pUL37m." evidence="4">
        <location>
            <begin position="178"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="488" mass="56088" checksum="7F9956DE7D43CCF2" modified="2004-07-05" version="1" precursor="true">MSPVYVNLLGSVGLLAFWYFSYRWIQRKRLEDPLPPWLRKKKACALTRRSRHRLRRQHGVIDGENSETERSVDLVAALLAEAGEESVTEDTEREDTEEEREDEEEENEARTPEVNPMDAEGLSGLAREACEALKKALRRHRFLWQRRRRARLLQHNGPQQSHHAAVFCRVHGLRGFQVSVWLLLTLFWSTGYGVSVRCTYHGTDINVTSNATSMNCRLNCTCNHTQIYNGPCAGAESKLPLNVTFRQSRRQWHSVMLTFGFQYHLEGWFPLRILNESRDINVTEVYGEVACFTNDTNITMGQLTLNLTGRSYVLRALARTSPFESSVNWEETNVTDNATSSENNTVTVMSVLTVYAESDYIFLQDMCPRFLKRSVKLAKNNRRNTTFTGTNVTSLPEWTLQQCQGWKYWTTLSIMWKNRRSALLRAKSRALGHWALLSICTVAAGSIALLSLFCILLIGLRRDLLEDFRYICRDEGSSSTKNDVHWIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW99</accession>
    <accession>D2K3K0</accession>
    <name>PP150_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Tegument protein pp150</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL32</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Participates in the last steps of viral maturation and release. Associates with nuclear capsids prior to DNA encapsidation and later preserves the integrity of nucleocapsids through secondary envelopment at the assembly compartment. Interacts with host CCNA2 and thereby blocks the onset of lytic gene expression to promote establishment of a quiescent state of infection in undifferentiated cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with host BICD1 and RAB6A. Interacts with small capsid protein UL48A; this interaction links together the capsid and pp150. Interacts with host CCNA2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host nucleus</location>
        </subcellularLocation>
        <text evidence="2">Found initially localized in the host nucleus, associated either with the nuclear membrane or with viral assembly regions, and later in the host cytoplasm.</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">Phosphorylated by host CCNA2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the herpesviridae large structural phosphoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081491.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW99"/>
    <dbReference type="PRIDE" id="Q6SW99"/>
    <dbReference type="DNASU" id="3077422"/>
    <dbReference type="GeneID" id="3077422"/>
    <dbReference type="KEGG" id="vg:3077422"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010340">
        <property type="entry name" value="Herpes_UL11/UL32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06070">
        <property type="entry name" value="Herpes_UL32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000418267" description="Tegument protein pp150">
        <location>
            <begin position="1"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="397"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="659"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1006"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="464"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="509"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="659"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="724"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="789"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="925"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="O-linked (GlcNAc) serine; by host" evidence="1">
        <location>
            <position position="922"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="O-linked (GlcNAc) serine; by host" evidence="1">
        <location>
            <position position="953"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P08318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="1049" mass="112759" checksum="871A6044F7406D84" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWA3</accession>
    <accession>D2K3J7</accession>
    <name>UL298_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL29/28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL29</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Contributes to activation of immediate-early gene expression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with UL38 and host HDAC1; these interactions are necessary for the HDAC1 interaction with UL38. Interacts with host MTA2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Expressed exclusively within the host nucleus in early times of infection and then present in both nucleus and cytoplasm at 72 hours post infection.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae US22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31593.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081487.2">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWA3"/>
    <dbReference type="GeneID" id="3077519"/>
    <dbReference type="KEGG" id="vg:3077519"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000417860" description="Protein UL29/28">
        <location>
            <begin position="1"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="700" mass="78971" checksum="845708E2851DDA72" modified="2009-03-24" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWA4</accession>
    <accession>D2K3J6</accession>
    <name>UL27_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL27</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Antiviral Res." volume="92" first="313" last="318">
            <title>Resistance to maribavir is associated with the exclusion of pUL27 from nucleoli during human cytomegalovirus infection.</title>
            <authorList>
                <person name="Hakki M."/>
                <person name="Drummond C."/>
                <person name="Houser B."/>
                <person name="Marousek G."/>
                <person name="Chou S."/>
            </authorList>
            <dbReference type="PubMed" id="21906628"/>
            <dbReference type="DOI" id="10.1016/j.antiviral.2011.08.019"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2011" name="Cell Host Microbe" volume="9" first="103" last="114">
            <title>Antiviral inhibition targeting the HCMV kinase pUL97 requires pUL27-dependent proteasomal degradation of Tip60 acetyltransferase and cell-cycle arrest.</title>
            <authorList>
                <person name="Reitsma J.M."/>
                <person name="Savaryn J.P."/>
                <person name="Faust K."/>
                <person name="Sato H."/>
                <person name="Halligan B.D."/>
                <person name="Terhune S.S."/>
            </authorList>
            <dbReference type="PubMed" id="21320693"/>
            <dbReference type="DOI" id="10.1016/j.chom.2011.01.006"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>INTERACTION WITH HOST PSME3; KAT5 AND EP400</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Promotes a cell cycle arrest in G0/G1 by inducing the proteasomal degradation of host histone acetyltransferase KAT5/Tip60.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with host KAT5, PSME3 and EP400.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Host nucleus</location>
            <location evidence="3">Host nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the herpesviridae U4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081486.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BioGRID" id="1678002">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWA4"/>
    <dbReference type="GeneID" id="3077449"/>
    <dbReference type="KEGG" id="vg:3077449"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010302">
        <property type="entry name" value="U5-like_protein_herpesevirus"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05999">
        <property type="entry name" value="Herpes_U5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1077">G0/G1 host cell cycle checkpoint dysregulation by virus</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1121">Modulation of host cell cycle by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418281" description="Protein UL27">
        <location>
            <begin position="1"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21320693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="21906628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="608" mass="69143" checksum="81CB97B6FDCF3F31" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWB8</accession>
    <accession>D2K3I2</accession>
    <name>UL13_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL13</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an essential role during infection by modulating mitochondrial ultrastructure and thereby increasing bioenergetic potential. Mechanistically, alters cristae architecture by interacting with components of the host mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host MICOS complex subunits IMMT and CHCHD3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HHV-5 UL13 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081472.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWB8"/>
    <dbReference type="DNASU" id="3077428"/>
    <dbReference type="GeneID" id="3077428"/>
    <dbReference type="KEGG" id="vg:3077428"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033650">
        <property type="term" value="C:host cell mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1045">Host mitochondrion</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000417837" description="Uncharacterized protein UL13">
        <location>
            <begin position="36"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="142"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="331"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="346"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="473" mass="54427" checksum="61A716C168B7B590" modified="2004-07-05" version="1" precursor="true">MLWAHCGRFLRYHLLPLLLCRLPFLLFFQRPQWAHGLDIVEEDEWLREIQGATYQLSIVRQAMQHAGFQVRAASVMTRRNAVDLDRPPLWSGSLPHLPVYDVRSPRPLRPPSSQHHAVSPELPSRNGIRWQYQELQYLVEEQRRRNQSRNAIPRPSFPPPDPPSQPAEDARDADAERAESPHTAESTVSHDASDNAVRRRHERRRYNALTVRSRDSLLLTRIRFSNQRCFGRGRLRHPAGSGPNTGGPRPGGAGLRQLRQQLTVRWQLFRLRCHGWTQQVSSQIRTRWEESNVVSQTATRVRTWFVQRTTLWRRTWVPGQNPAAEAQELAIIPPAPTVLRQNEEPRQQLTGEETRNSTHTQREEVEDVSREGAREGNDGSRASGNDERRNNAGRYDDDHEVQEPQVTYPAGQGELNRRSQEENEEGGPCESPPMTTNTLTVACPPREPPHRALFRLCLGLWVSSYLVRRPMTI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWB9</accession>
    <accession>D2K3I1</accession>
    <name>UL11P_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL11</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="PLoS Pathog." volume="7" first="E1002432" last="E1002432">
            <title>The human cytomegalovirus UL11 protein interacts with the receptor tyrosine phosphatase CD45, resulting in functional paralysis of T cells.</title>
            <authorList>
                <person name="Gabaev I."/>
                <person name="Steinbrueck L."/>
                <person name="Pokoyski C."/>
                <person name="Pich A."/>
                <person name="Stanton R.J."/>
                <person name="Schwinzer R."/>
                <person name="Schulz T.F."/>
                <person name="Jacobs R."/>
                <person name="Messerle M."/>
                <person name="Kay-Fedorov P.C."/>
            </authorList>
            <dbReference type="PubMed" id="22174689"/>
            <dbReference type="DOI" id="10.1371/journal.ppat.1002432"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HOST PTPRC</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2014" name="J. Virol." volume="88" first="14326" last="14339">
            <title>Expression of the human cytomegalovirus UL11 glycoprotein in viral infection and evaluation of its effect on virus-specific CD8 T cells.</title>
            <authorList>
                <person name="Gabaev I."/>
                <person name="Elbasani E."/>
                <person name="Ameres S."/>
                <person name="Steinbrueck L."/>
                <person name="Stanton R."/>
                <person name="Doering M."/>
                <person name="Lenac Rovis T."/>
                <person name="Kalinke U."/>
                <person name="Jonjic S."/>
                <person name="Moosmann A."/>
                <person name="Messerle M."/>
            </authorList>
            <dbReference type="PubMed" id="25275132"/>
            <dbReference type="DOI" id="10.1128/JVI.01691-14"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>GLYCOSYLATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4">Plays a role in the modulation of host immune response by modulating T-cell function. Interacts with host PTPRC/CD45 and thereby reduces host TCR signaling and T-cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with host PTPRC; this interaction affects T-cell signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host cell membrane</location>
            <topology evidence="5">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host endoplasmic reticulum</location>
        </subcellularLocation>
        <text>Localizes to the host cell membrane when highly glycosylated while less glycosylated forms are found on the endoplasmic reticulum.</text>
    </comment>
    <comment type="PTM">
        <text evidence="4">Glycosylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081471.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWB9"/>
    <dbReference type="DNASU" id="3077560"/>
    <dbReference type="GeneID" id="3077560"/>
    <dbReference type="KEGG" id="vg:3077560"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="SSF48726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418313" description="Protein UL11">
        <location>
            <begin position="32"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="32"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="22174689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="25275132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="272" mass="30881" checksum="50B9D252EB1EC5DB" modified="2004-07-05" version="1" precursor="true">MLFRYITFHREKVLYLTAACIFGVYISLHDACIPVVGKIGTNVTLNAVDVLPPRDQVRWSYGPGGQGYMLCIFTGTSTTTFNNTRFNFSCLSNYSLLLINVTTQYSTTYRTMTSLDHWLHQRHNHGSRWTLDTCYNLTVNENGTFPTTTTKKPTTTTRTTTTTTQRTTTTRTTTTAKKTTISTTHHKHPSPKKSTTPNSHVEHHVGFEATAAETPLQPSPQHQHLATHALWVLAVVIVIIIIIIFYFRIPQKLWLLWQHDKHGIVLIPQTDL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWC0</accession>
    <accession>D2K3I0</accession>
    <name>UL10_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL10</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081470.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWC0"/>
    <dbReference type="DNASU" id="3077476"/>
    <dbReference type="GeneID" id="3077476"/>
    <dbReference type="KEGG" id="vg:3077476"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418275" description="Uncharacterized protein UL10">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="254" mass="29023" checksum="C0D9B8BCBB70EFE3" modified="2004-07-05" version="1">MRQLINHIVNHDLFRWSVVTAMIFYRYSETCMEVTVRVGDPVTLGSGHGYHPGQKVHWYNQSCVGIGNGENTHPICTYDPPKPGRQKTMKTTPLPSPLLYECHNSTLSILHVNVSDPRNYCRRKCPPKGNCEFPTCFTLSLISRTTTRKPEQKTTLLRLKTTPNKHTQHKRSTRRTSPKDYNVTGLPKGFADSFTGNVEAHRTKDAAHSAWILIIIIIIIVVILFFFKIPQRLREKWDTKGYLYKGTDGLPTTD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWC3</accession>
    <accession>D2K3H7</accession>
    <name>UL07_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>CEACAM1-like protein UL7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL7</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2014" name="MBio" volume="5" first="E02035" last="E02035">
            <title>Human cytomegalovirus-encoded pUL7 is a novel CEACAM1-like molecule responsible for promotion of angiogenesis.</title>
            <authorList>
                <person name="MacManiman J.D."/>
                <person name="Meuser A."/>
                <person name="Botto S."/>
                <person name="Smith P.P."/>
                <person name="Liu F."/>
                <person name="Jarvis M.A."/>
                <person name="Nelson J.A."/>
                <person name="Caposio P."/>
            </authorList>
            <dbReference type="PubMed" id="25352622"/>
            <dbReference type="DOI" id="10.1128/mBio.02035-14"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in modulating the host immune response and affecting host cytokine production. Structurally and functionally homolog of host CEACAM1, induces endothelial cell angiogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cell membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Highly glycosylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081467.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SWC3"/>
    <dbReference type="PRIDE" id="Q6SWC3"/>
    <dbReference type="DNASU" id="3077480"/>
    <dbReference type="GeneID" id="3077480"/>
    <dbReference type="KEGG" id="vg:3077480"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="SSF48726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1118">Modulation of host dendritic cell activity by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="chain" id="PRO_0000417835" description="CEACAM1-like protein UL7">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="25352622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="222" mass="24322" checksum="13B625E689AC5013" modified="2004-07-05" version="1">MASDVGSHPLTVTRFRCRVHYVYNKLLILTLFAPVILESVIYVSGPQGGNVTLVSNFTSNISARWFRWDGNDSHLICFYKRGEGLSTPYVGLSLSCAANQITIFNLTLNDSGRYGAEGFTRSGENETFLWYNLTVKPKPLETTPASNVTTIVTTTSTVTDAKSNVTGNVSLAPQLRAVAGFSHQTPLENNTHLALVGVVVFLVLIVVCIMGWWKLLCSKSEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWC4</accession>
    <accession>D2K3H6</accession>
    <name>UL06_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL6</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081466.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SWC4"/>
    <dbReference type="PRIDE" id="Q6SWC4"/>
    <dbReference type="DNASU" id="3077507"/>
    <dbReference type="GeneID" id="3077507"/>
    <dbReference type="KEGG" id="vg:3077507"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000417834" description="Uncharacterized protein UL6">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="280" mass="30921" checksum="AFDDC26E7F6FE686" modified="2004-07-05" version="1">MNGWAGVCLITHCLNTRSRTYVALNMLAFARTPRGVPSCLFNKVWVSRYALVLILMVCASESSTSWAVTSNGLPNCSTVTRTAGQDAELHGPAPLSCNVTQWGRYENGSTPVLWCTLRGSRMRVSLGHRVAFGCSWKTFFIYNVSESSGGTYYQKGYNCTDKHITLSCFNLTVVPRAVQSTTTVMTPTLVTNSTFSVSLVALRLTTNSSAFGHAIYQRQQRVENGTLSKNITNLAFTYGSWGVAMLLFAAVMVLVDLGLPQSAWRRWRSHVDDEERGLLM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWC9</accession>
    <accession>D2K3H1</accession>
    <name>RL13_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane protein RL13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RL13</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="J. Clin. Invest." volume="120" first="3191" last="3208">
            <title>Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication.</title>
            <authorList>
                <person name="Stanton R.J."/>
                <person name="Baluchova K."/>
                <person name="Dargan D.J."/>
                <person name="Cunningham C."/>
                <person name="Sheehy O."/>
                <person name="Seirafian S."/>
                <person name="McSharry B.P."/>
                <person name="Neale M.L."/>
                <person name="Davies J.A."/>
                <person name="Tomasec P."/>
                <person name="Davison A.J."/>
                <person name="Wilkinson G.W."/>
            </authorList>
            <dbReference type="PubMed" id="20679731"/>
            <dbReference type="DOI" id="10.1172/JCI42955"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <comment type="function">
        <text>May play a role in modifying tropism or in modulating cell signaling during virus entry. Since RL13 expression severely impairs HCMV replication in epithelial cell cultures, it may act as a regulator promoting persistence by suppressing the switch to fully lytic infection.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081461.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWC9"/>
    <dbReference type="DNASU" id="3077492"/>
    <dbReference type="GeneID" id="3077492"/>
    <dbReference type="KEGG" id="vg:3077492"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="SSF48726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000417862" description="Membrane protein RL13">
        <location>
            <begin position="20"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="293" mass="33000" checksum="4AA06E2B8B3D328D" modified="2004-07-05" version="1" precursor="true">MHWHLAITWTVIILTFSECYNQTCPCPCICVNSTTVSISTSETTSKNITPTTTTNSKKTTSSIATTTPSLVTTGKVVSTAASSTIISQTNRSHTSNAITTPKTRFEYNITGYVGQEVTLNFTGSWNYIQWFRYGSPGWLYSSEPICTVTSNYHHTFPRGALCFDCDMTKLLIYDLTLNDSGKYVVKRTRHDNQYEEACYSLTVIFANTTSIVTNRTCDRKRTENTDTTNHEIGKHIIETIKKANIPLGIHAVWAGIVVSVALIALYMGNRRRPRKPRYTRLPKYDPDESWTKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWD0</accession>
    <accession>D2K3H0</accession>
    <name>RL12_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein RL12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RL12</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081460.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWD0"/>
    <dbReference type="DNASU" id="3077488"/>
    <dbReference type="GeneID" id="3077488"/>
    <dbReference type="KEGG" id="vg:3077488"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000417861" description="Uncharacterized protein RL12">
        <location>
            <begin position="1"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="367"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="414" mass="46600" checksum="0D98B2B9B0972F9F" modified="2004-07-05" version="1">MRVDRQKRNNFTYRQTVYIILTFYIVYRGKCNSTDTNNSTSTSDSTVSDTSVYSTPNLPSTFFTTLDTSTGSQISTTSNTISSTTNTLAASSITTLNTSTNTTSYTASTLTALSSTHTNSPILSNNATLYTTSLDNTTTDITSSESSINASTIHNTTYSPVTSIAVNCTVAANETNNSSSKNCQQDIGTIRVKSTTLTAEEGKNITIQGNSTWNCPNVVWYRHYNWSTHGHHIYPNRRYQTPTYQHKILTSHPICHPDVSSPAAYHDLCRSCNKTELRIYDLNTTNSGRYSRRCYKEYNHDGPHEDENFGLTVNPRNDTDNHTTPLCPRYVGTQSEEDEDDDYTLSTITNNNMRKTSHRDISHGTRTTWALTLICIACILLFFVRRALNKRYRPLRDDISESSFVVRYHPEHED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWD1</accession>
    <accession>D2K3G9</accession>
    <name>RL11_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane glycoprotein RL11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RL11</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081459.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWD1"/>
    <dbReference type="DNASU" id="3077515"/>
    <dbReference type="GeneID" id="3077515"/>
    <dbReference type="KEGG" id="vg:3077515"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418305" description="Membrane glycoprotein RL11">
        <location>
            <begin position="24"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="234" mass="26690" checksum="14141BBA085F3080" modified="2004-07-05" version="1" precursor="true">MQTYSTPLTLIIVTSLFLFTTQGSSSNAVEPTKKPLKLANYRATCEDRTRTLVTRLNTSHHSVVWQRYDIYSRYMRRMPPLCIITDAYKETTHQGGATFTCTRQNLTLYNLTVKDTGVYLLQDQYTGDVEAFYLIIHPRSFCRALETRRCFYPGPGRVVVTDSQEADRAIISDLKRQWSGLSLHCAWVSGLMIFVGALVICFLRSQRIGEQDAEQLRTDLDTEPLLLTVDGDLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>C1BEG3</accession>
    <name>UL74A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL74A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL74A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="ACO83398.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_002802310.2">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="C1BEG3"/>
    <dbReference type="DNASU" id="7755302"/>
    <dbReference type="GeneID" id="7755302"/>
    <dbReference type="KEGG" id="vg:7755302"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000418306" description="Uncharacterized protein UL74A">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="70" mass="7663" checksum="BD4951FC46EE123C" modified="2011-09-21" version="2">MALTRGGDPAPSVCLVVWFACVYSLLILVVLLLIYRCCIGFQDDLVSRTLAVYRACIQGPICNQTHNSTS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5H8Q3</accession>
    <name>UL148_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane protein UL148</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL148</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Chaperone protein that plays an important role in HCMV tropism. Cooperates with UL116 to regulate the abundance of gH-gL complexes in virion. Favors the incorporation of gL into virions once UL116 has regulated the early folding steps of virion assembly. Interacts with the host ERAD machinery and slows gO decay which would otherwise be constitutively degraded. Reorganizes the host endoplasmic reticulum and activates the unfolded protein response. Additionally, plays a role in the evasion of antiviral immune response by down-regulating cell surface expression of host CD58. Mechanistically, interacts with host CD58 and retains its immature form intracellularly. The capacity to cause endoplasmic reticulum reorganization and the intracellular retention of host CD58 are functionally independent properties.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host SEL1L.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081568.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="DNASU" id="3077479"/>
    <dbReference type="GeneID" id="3077479"/>
    <dbReference type="KEGG" id="vg:3077479"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044167">
        <property type="term" value="C:host cell endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039503">
        <property type="term" value="P:suppression by virus of host innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418261" description="Membrane protein UL148">
        <location>
            <begin position="21"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="B8YEE5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <sequence length="316" mass="36504" checksum="8B6BFA7ADB6ACF13" modified="2011-06-28" version="1" precursor="true">MLRLLFTLVLLALYGPSVDASRDYVHVRLLSYRGDPLVFKHTFSGVRRPFTELGWAACRDWDSMHCTPFWSTDLEQMTDSVRRYSTVSPGKEVTLQLHGNQTVQPSFLSFTCRLQLEPVVENVGLYVAYVVNDGERPQQFFTPQVDVVRFALYLETLSRIVEPLESGRLTVEFDTPDLALAPDLVSSLFVAGHGETDFYMNWTLRRSQTHYLEEMALQVEILKPRGVRHRAIIHHPKLQPGVGLWIDFCVYRYNARLTRGYVRYTLSPKARLPAKAEGWLVSLDRFIVQYLNTLLITMMAAIWARVLITYLVSRRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F5H8S6</accession>
    <name>US24_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Tegument protein US24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US24</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae US22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081609.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="GeneID" id="3077578"/>
    <dbReference type="KEGG" id="vg:3077578"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000418254" description="Tegument protein US24">
        <location>
            <begin position="1"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="501" mass="58026" checksum="8CE942CD80ACB80B" modified="2011-06-28" version="1">MMDPAAGSGPDGAAVVPPELPALPVAAEDPMALYRQVLRDFKELFFCLEPMEITRYVHRNEGRCLSLGPPKGWHVMLRTEDGIITAAKQAASKLICCREPLTPLGYAVILLPEPRRDHHDGMVATPYVVFMGRFSRVYAYDTREKYMVLVSHNLDELARYGVSRSEIAYRDVIHTTLRRMTVPVPRRYPKGARTMHVLFLNDTTPEGSYATAERILGCDVKLHTPGYGTVIMRLMKTVQQLHRIWPFCALTEVESRRWWWAVRANLATPWYVLGVTGRPRPGRSFVAEVLVLLDWFGAVYAIQMDDPNHYVRRVANTITEFFRMGLLKMVFRHRRFERERQRQTRMEHRHLCPHHHERAVDHKRDILFNEDAALPDERRERERRILQQQYDWLCLTERFDPHEGAWERLDPNTLVLHRYDTNSQSYVLDPDIVGVEAAEREAAGHQDDTGPRLHCLVTTRSSTREGAERVITALVHQSRLVTYSDPFPLKSLTGVREYIQI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F5H9I4</accession>
    <name>US13_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane protein US13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US13</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytomegalovirus US12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081598.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5H9I4"/>
    <dbReference type="DNASU" id="3077576"/>
    <dbReference type="GeneID" id="3077576"/>
    <dbReference type="KEGG" id="vg:3077576"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418252" description="Membrane protein US13">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="261" mass="29460" checksum="9E4C732BE5D91900" modified="2011-06-28" version="1">MDPPLPSLHSPQWASLLQLHHGLMWLRRFAVLVRVYALVVFHIAISTAFCGMIWLGIPDSHNICQHESSPLLLVFAPSLLWCLVLIQGERHPDDVVLTMGYVGLLSVTTVFYTWCSDLPAILIDYTLVLTLWIACTGAVMVGDSFRAKRWELICSRVLTSVFFITLWVIGDQTVFHHQRILLYGYGAIVFLMMTVTFYGTRYIRDELPAAQTLRGSLLIYVGLVTMFKITLIVLSPNLWRLPWTTVFAAFRSSYCEGGGGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F5HA10</accession>
    <name>CEP1_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytoplasmic envelopment protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL103</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="J. Virol." volume="85" first="5125" last="5135">
            <title>Cytomegalovirus UL103 controls virion and dense body egress.</title>
            <authorList>
                <person name="Ahlqvist J."/>
                <person name="Mocarski E."/>
            </authorList>
            <dbReference type="PubMed" id="21345947"/>
            <dbReference type="DOI" id="10.1128/JVI.01682-10"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>Towne</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Plays a critical role in cytoplasmic virus egress. Participates in the final step of tegumentation and envelope acquisition within the host cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 2">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 2">Host Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae cytoplasmic envelopment protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081549.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HA10"/>
    <dbReference type="GeneID" id="3077559"/>
    <dbReference type="KEGG" id="vg:3077559"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04038">
        <property type="entry name" value="HSV_CEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002600">
        <property type="entry name" value="Herpes_UL7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01677">
        <property type="entry name" value="Herpes_UL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000418241" description="Cytoplasmic envelopment protein 1">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21345947"/>
        </source>
    </evidence>
    <sequence length="249" mass="28636" checksum="A8D9F8F89F02FE9D" modified="2011-06-28" version="1">MEALMIRGVLEVHTDFTRQNVMIMEPQVLDFTVRGDKLWLHTEHGLLVSMAEYRSELLCTSAFLGYSAVFLLETEDAVTQVRLSDLRLKHRCGIVKADNLLHFALCTVISCVENCNLTRKCLHDLLQYLDAVNVRESFGRLLHHSARRLICSALYLLFEEKEPHIVQYVPATFVLFQQTRHTCLQLVARFFFRLTGQDEAHSFSLKLTERKTVDGWPVGLGLLDVLNANYPNLPSPPKLPPRWERGEEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>F5HAK6</accession>
    <name>U148B_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL148B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL148B</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081583.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HAK6"/>
    <dbReference type="DNASU" id="3077453"/>
    <dbReference type="GeneID" id="3077453"/>
    <dbReference type="KEGG" id="vg:3077453"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418262" description="Protein UL148B">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="80" mass="8869" checksum="B5B66E54C80BBCB8" modified="2011-06-28" version="1">MALDTLAGLAICVGLVMGVTVIASCALLVFYYCDEREDGRPSKLLQRSIRRWRHGLSTESLTAILPDGSSTEQEIYHTRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-12-14" modified="2022-02-23" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F5HAM0</accession>
    <name>UL144_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane glycoprotein UL144</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>TNF alpha-like receptor UL144</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>UL144 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">UL144</name>
        <name type="ORF">HHV5wtgp119</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Activates NF-kappa-B in a tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6)-dependent manner, causing the up-regulation of the chemokine CCL22.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host TRIM23; this interaction causes auto-ubiquitination of TRAF6, leading to NF-kappaB activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081573.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HAM0"/>
    <dbReference type="BioGRID" id="1678024">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HAM0"/>
    <dbReference type="DNASU" id="3077471"/>
    <dbReference type="GeneID" id="3077471"/>
    <dbReference type="KEGG" id="vg:3077471"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00020">
        <property type="entry name" value="TNFR_c6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1074">Activation of host NF-kappa-B by virus</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000414120" description="Membrane glycoprotein UL144">
        <location>
            <begin position="21"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys">
        <location>
            <begin position="58"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="176" mass="19629" checksum="B6726B043F38775B" modified="2011-06-28" version="1" precursor="true">MKPLVMLICFGVILLQLGVTKVCQHNEVQLGNECCPPCGLGQRVTKVCTERTSVTCTPCPNGTYVSGLYNCTDCTQCNVTQVMIRNCTSTNNTVCAPKNHTYFSTPGVQHHKQRQQNHTAHITVKQGKSGRHTLAWLSLFIFLVGIILLILYLIAAYRSERCQQCCSIGKIFYRTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5HAR3</accession>
    <name>US19_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Transmembrane protein US19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US19</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="developmental stage">
        <text>Expressed 34 hours post-infection.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytomegalovirus US12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081604.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HAR3"/>
    <dbReference type="DNASU" id="3077522"/>
    <dbReference type="GeneID" id="3077522"/>
    <dbReference type="KEGG" id="vg:3077522"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0426">Late protein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000416727" description="Transmembrane protein US19">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="240" mass="26413" checksum="B9D78CD10E27F494" modified="2011-06-28" version="1">MLHVVPLEWTVEEVVPYLERLAVWLRASVLVAFQLTATVALSVLSWWLMPPPVAELCERGRDDDPPSLSHLSLVVPVGCLFLLLRGPSIDRCPRKLPLLLAYCLPHALAFLTLLMCQPSPQAFVGAALLALAVDLSCLGASLLGCDPGASLRRLWLPSVLSLLCATALGLWLLRAAAPFFLGLHATTLLTVTLMLIHDLSLITCQSSFPESFQPSLRLYVENVALFIGMYHLLRLWLWSP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F5HAZ3</accession>
    <name>US23_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein US23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US23</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion tegument</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae US22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081608.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HAZ3"/>
    <dbReference type="GeneID" id="3077446"/>
    <dbReference type="KEGG" id="vg:3077446"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000416728" description="Protein US23">
        <location>
            <begin position="1"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="592" mass="68886" checksum="BF48A1B9E11DDEB6" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-02-23" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F5HB53</accession>
    <name>GB_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Envelope glycoprotein B</fullName>
            <shortName evidence="3">gB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gB</name>
        <name type="ORF">UL55</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan sulfate moieties of the host cell surface proteoglycans. Involved in fusion of viral and cellular membranes leading to virus entry into the host cell. Following initial binding to its host receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May be involved in the fusion between the virion envelope and the outer nuclear membrane during virion egress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer; disulfide-linked. Binds to heparan sulfate proteoglycans. Interacts with gH/gL heterodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Virion membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Host cell membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Host endosome membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Host Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <text evidence="3">During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN).</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">A proteolytic cleavage by host furin generates two subunits that remain linked by disulfide bonds.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae glycoprotein B family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081514.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HB53"/>
    <dbReference type="TCDB" id="1.G.22.1.1">
        <property type="family name" value="the cytomegalovirus (human herpesvirus 5) glycoprotein go (go) family"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HB53"/>
    <dbReference type="DNASU" id="3077424"/>
    <dbReference type="GeneID" id="3077424"/>
    <dbReference type="KEGG" id="vg:3077424"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04032">
        <property type="entry name" value="HSV_GB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021044">
        <property type="entry name" value="Glycoprot_B_antigenic_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035377">
        <property type="entry name" value="Glycoprot_B_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035381">
        <property type="entry name" value="Glycoprot_B_PH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038631">
        <property type="entry name" value="Glycoprot_B_PH2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000234">
        <property type="entry name" value="Herpes_Glycoprot_B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17416">
        <property type="entry name" value="Glycoprot_B_PH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17417">
        <property type="entry name" value="Glycoprot_B_PH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12154">
        <property type="entry name" value="HCMVantigenic_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1039">Host endosome</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-1161">Viral attachment to host cell</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416440" description="Envelope glycoprotein B" evidence="3">
        <location>
            <begin position="25"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="3">
        <location>
            <begin position="25"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="752"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="3">
        <location>
            <begin position="773"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in fusion and/or binding to host membrane" evidence="3">
        <location>
            <begin position="152"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in fusion and/or binding to host membrane" evidence="3">
        <location>
            <begin position="237"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Hydrophobic membrane proximal region" evidence="3">
        <location>
            <begin position="697"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="798"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="857"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Internalization motif" evidence="3">
        <location>
            <begin position="895"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="798"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="859"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="882"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by host furin" evidence="2">
        <location>
            <begin position="460"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="447"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="466"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="555"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="586"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="94"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="111"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="185"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="344"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="574"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="907" mass="101885" checksum="C2CE17E8C6E92B00" modified="2011-06-28" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F5HBX1</accession>
    <name>CXCL1_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Chemokine vCXCL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL146</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="Proc. Natl. Acad. Sci. U.S.A." volume="96" first="9839" last="9844">
            <title>Cytomegalovirus encodes a potent alpha chemokine.</title>
            <authorList>
                <person name="Penfold M.E."/>
                <person name="Dairaghi D.J."/>
                <person name="Duke G.M."/>
                <person name="Saederup N."/>
                <person name="Mocarski E.S."/>
                <person name="Kemble G.W."/>
                <person name="Schall T.J."/>
            </authorList>
            <dbReference type="PubMed" id="10449781"/>
            <dbReference type="DOI" id="10.1073/pnas.96.17.9839"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>Toledo</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="J. Biol. Chem." volume="285" first="9137" last="9146">
            <title>The cytomegalovirus UL146 gene product vCXCL1 targets both CXCR1 and CXCR2 as an agonist.</title>
            <authorList>
                <person name="Luttichau H.R."/>
            </authorList>
            <dbReference type="PubMed" id="20044480"/>
            <dbReference type="DOI" id="10.1074/jbc.M109.002774"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH CXCR1 AND CXCR2</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Acts as a functional chemokine, inducing calcium mobilization, chemotaxis, and degranulation of neutrophils. Contributes to the induction of neutrophil chemotaxis by interacting with host CXCR1 and CXCR2 receptors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with host CXCR1 and CXCR2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the intercrine alpha (chemokine CxC) family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081571.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HBX1"/>
    <dbReference type="DNASU" id="3077566"/>
    <dbReference type="GeneID" id="3077566"/>
    <dbReference type="KEGG" id="vg:3077566"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039553">
        <property type="term" value="P:suppression by virus of host chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="SSF54117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1086">Inhibition of host chemokines by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="chain" id="PRO_0000418259" description="Chemokine vCXCL1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="10449781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="20044480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="120" mass="13872" checksum="D5247AF226EA2093" modified="2011-06-28" version="1">MRLIFGALIISLTYMYYYEVHGTELRCKCLDGKKLPPKTIMLGNFWFHRESGGPRCNNNEYFLYLGGGKKHGPGVCLSPHHPFSKWLDKRNDNRWYNVNVTRQPERGPGKITVTLVGLKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F5HC16</accession>
    <name>UL34_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Transcriptional regulator UL34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL34</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a transcriptional repressor of the US3 gene expression through a specific DNA sequence named the transcriptional repressive element (tre).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 UL34 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081493.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HC16"/>
    <dbReference type="DNASU" id="3077500"/>
    <dbReference type="GeneID" id="3077500"/>
    <dbReference type="KEGG" id="vg:3077500"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000416717" description="Transcriptional regulator UL34">
        <location>
            <begin position="1"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="407" mass="45403" checksum="D77DCC2F14587A00" modified="2011-06-28" version="1">MNFIITTRDFSNDDSVLRAAEMRDNVAGSISKAYKGTVRAEGKKKLLLKHLPVPPGGCSRRNSNLFVFCTERDYRKFHQGIAQLKRAPAELDPHEIQQVTASIRCRLQPSLREPPTPADELQTAVSRVCALFNQLVFTAQLRHYCEHQDKVVSYARDELTKRCGEKSALGVEVHQLVALLPHERHRELCHVLIGLLHQTPHMWARSIRLIGHLRHYLQNSFLHLLMNSGLDIAQVFDGCYHSEAYRMLFQIGHTDSVSAALELSHSAAAGPPEADENNDEGEEDDDELRHSDPAPLHDSKKPRNARRPRTRVPPHEQKPEENEEEEEELFPSCKATAAFLRAEPSVSNDDGNGGERCDTLATALRHRADEEDGPLASQTSVRVAATPSPSVTSALTPVTSPITPLCI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F5HC79</accession>
    <name>TRM1_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tripartite terminase subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">TRM1</name>
        <name type="ordered locus">UL56</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1426" last="1433">
            <title>Identification of the ATP-binding site in the terminase subunit pUL56 of human cytomegalovirus.</title>
            <authorList>
                <person name="Scholz B."/>
                <person name="Rechter S."/>
                <person name="Drach J.C."/>
                <person name="Townsend L.B."/>
                <person name="Bogner E."/>
            </authorList>
            <dbReference type="PubMed" id="12595550"/>
            <dbReference type="DOI" id="10.1093/nar/gkg229"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>DOMAIN</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="Antivir. Ther." volume="13" first="643" last="654">
            <title>Putative functional domains of human cytomegalovirus pUL56 involved in dimerization and benzimidazole D-ribonucleoside activity.</title>
            <authorList>
                <person name="Champier G."/>
                <person name="Couvreux A."/>
                <person name="Hantz S."/>
                <person name="Rametti A."/>
                <person name="Mazeron M.C."/>
                <person name="Bouaziz S."/>
                <person name="Denis F."/>
                <person name="Alain S."/>
            </authorList>
            <dbReference type="PubMed" id="18771048"/>
        </citation>
        <scope>DOMAIN</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">Component of the molecular motor that translocates viral genomic DNA in empty capsid during DNA packaging. Forms a tripartite terminase complex together with TRM2 and TRM3 in the host cytoplasm. Once the complex reaches the host nucleus, it interacts with the capsid portal vertex. This portal forms a ring in which genomic DNA is translocated into the capsid. TRM1 carries an endonuclease activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with TRM2 and TRM3 to form the tripartite terminase complex. Interacts with portal protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <text evidence="1">Found associated with the external surface of the viral capsid during assembly and DNA packaging, but seems absent in extracellular mature virions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae TRM1 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081515.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HC79"/>
    <dbReference type="DrugBank" id="DB12070">
        <property type="generic name" value="Letermovir"/>
    </dbReference>
    <dbReference type="DrugCentral" id="F5HC79"/>
    <dbReference type="PRIDE" id="F5HC79"/>
    <dbReference type="GeneID" id="3077457"/>
    <dbReference type="KEGG" id="vg:3077457"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019069">
        <property type="term" value="P:viral capsid assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019073">
        <property type="term" value="P:viral DNA genome packaging"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019076">
        <property type="term" value="P:viral release from host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04014">
        <property type="entry name" value="HSV_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000501">
        <property type="entry name" value="UL28/UL56"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01366">
        <property type="entry name" value="PRTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0426">Late protein</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0231">Viral genome packaging</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000436183" description="Tripartite terminase subunit 1">
        <location>
            <begin position="1"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1 4">
        <location>
            <begin position="191"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="438"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="799"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal" evidence="1 4">
        <location>
            <begin position="822"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 3">
        <location>
            <begin position="709"/>
            <end position="716"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12595550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="18771048"/>
        </source>
    </evidence>
    <sequence length="850" mass="95811" checksum="DB388A772024D113" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>F5HCK7</accession>
    <name>UL140_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL140</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL140</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081576.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HCK7"/>
    <dbReference type="PRIDE" id="F5HCK7"/>
    <dbReference type="DNASU" id="3077467"/>
    <dbReference type="GeneID" id="3077467"/>
    <dbReference type="KEGG" id="vg:3077467"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000417838" description="Protein UL140">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="191" mass="21466" checksum="E4B9139F4EB31C48" modified="2011-06-28" version="1">MTPAQTNGTTTVHPHGAKNGSGGSALPTLVVFGFIVTLLFFLFMLYFWNNDVFRKLLRCAWIQRCCDRFDAWQDEVIYRRPSRRSQSDDESRTNSVSSYVLLSPASDGGFDNPALTEAVDSVDDWATTSVFYATSDETADTERRDSQQLLIELPPEPLPPDVVAAMQKAVKRAVQNALRHSHDSWQLHQTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5HD92</accession>
    <name>US14_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane protein US14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US14</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytomegalovirus US12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081599.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HD92"/>
    <dbReference type="DNASU" id="3077456"/>
    <dbReference type="GeneID" id="3077456"/>
    <dbReference type="KEGG" id="vg:3077456"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000416725" description="Membrane protein US14">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="310" mass="34297" checksum="F56088ED5258B625" modified="2011-06-28" version="1">METVSTQRETASSETERTREAASAETTDATFRSLEEGSTISSRYSETASTASEDAVCWLRRTAIVMRVYGLLTLETAFSVLISALVWLGYPSLGYECRDDPSPLLLSCTPVLVLGALELTDHRHPSNGLVFALYVALLSFTTAGLNLCATAPIGVSSLILTWTLFVACNGVAWEHRLSSVWRDALFTSTLLTVMVSVLASTYTWLHKTLLCLYTVFVGCILAVLFQDVRYIATKMPVSHIIRSSLVLYATETLIYHTTLLMLTPVVWSARWDQMFSYLAKLGTYHHYRVDNGTLSVILNSTTATFQSRVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F5HDC7</accession>
    <name>US22_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Tegument protein US22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US22</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae US22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081607.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HDC7"/>
    <dbReference type="GeneID" id="3077463"/>
    <dbReference type="KEGG" id="vg:3077463"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000418295" description="Tegument protein US22">
        <location>
            <begin position="1"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="471"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="477"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="495"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="576" mass="65048" checksum="18C43B2E3A8143B0" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>F5HDE7</accession>
    <name>U148C_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL148C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL148C</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081584.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HDE7"/>
    <dbReference type="PRIDE" id="F5HDE7"/>
    <dbReference type="DNASU" id="3077427"/>
    <dbReference type="GeneID" id="3077427"/>
    <dbReference type="KEGG" id="vg:3077427"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418300" description="Protein UL148C">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="77" mass="8527" checksum="F90FED70FD558CFB" modified="2011-06-28" version="1">MLTPAVFPAVLYLLALVVWVEMFCLVAVAVVEREIAWALLLRMLVVGLMVEVGAAAAWTFVRCLAYQRSFPVLTAFP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F5HE69</accession>
    <name>US18_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Transmembrane protein US18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US18</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytomegalovirus US12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081603.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HE69"/>
    <dbReference type="GeneID" id="3077472"/>
    <dbReference type="KEGG" id="vg:3077472"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0426">Late protein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000416726" description="Transmembrane protein US18">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="274" mass="30194" checksum="253FFEF64DEC721B" modified="2011-06-28" version="1">MGDTASVSEHHESPTVTIVPLHRSHALVAEQQLFQWLKRFKLLMEVYHGLVWQLACTLTVCLLAWLAFPDVQGQCANGIVPALSSIVPVSTLAMLRGFAEFRPHTTNFAHLTVACLLINTGITVCTGFCGERRVIGLSFALVMVFFVLCSGLTYLAGNNPTRWKVIGIGYGWSVIVFYLLLYFSPVLWVSKIYSGLYVLVVTAASAVLIYETLDLIYQRGTLSKNSVCVSVVLYTIVMSLLNMSVAIFSGHVWVQQYAEKHGGRIDGVSLLSLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2021-09-29" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F5HEA3</accession>
    <name>TEG7_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Tegument protein UL51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL71</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays several roles during the time course of infection, including egress of virus particles from the perinuclear space and secondary envelopment of cytoplasmic capsids that bud into specific trans-Golgi network (TGN)-derived membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Oligomerizes. Interacts with UL103; this interaction mediates UL103 incorporation to virions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Palmitoylation is necessary for Golgi localization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the herpesviridae UL51 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081519.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HEA3"/>
    <dbReference type="DNASU" id="3077537"/>
    <dbReference type="GeneID" id="3077537"/>
    <dbReference type="KEGG" id="vg:3077537"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007619">
        <property type="entry name" value="Herpes_U44"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04533">
        <property type="entry name" value="Herpes_U44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0564">Palmitate</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000417846" description="Tegument protein UL51 homolog">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="251"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="285"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-palmitoyl cysteine; by host" evidence="1">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P10235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P16823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="361" mass="39860" checksum="27E5EF8F36298BD6" modified="2011-06-28" version="1">MQLAQRLCELLMCRRKAAPVADYVLLQPSEDVELRELQAFLDENFKQLEITPADLRTFSRDTDVVNHLLKLLPLYRQCQSKCAFLKGYLSEGCLPHTRPAAEVECKKSQRILEALDILILKLVVGEFAMSEADSLEMLLDKFSTDQASLVEVQRVMGLVDMDCEKSAYMLEAGAAATVAPPTPPAVVQGESGVREDGETVAAVSAFACSSVSDSLIPEETGVTRPMMSLAHINTVSCPTVMRFDQRLLEEGDEEDEVTVMSPSPEPVQQQPPVEPVQQQPQGRGSHRRRYKESAPQETLPTNHEREILDLMRHSPDVPREAVMSPTMVTIPPPQIPFVGSARELRGVKKKKPTAAALLSSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F5HEF3</accession>
    <name>US34_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein US34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US34</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HHV-5 US34 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081617.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HEF3"/>
    <dbReference type="GeneID" id="13229472"/>
    <dbReference type="KEGG" id="vg:3077433"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416730" description="Protein US34">
        <location>
            <begin position="23"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="163" mass="17453" checksum="2B9760A2A485F28A" modified="2011-06-28" version="1" precursor="true">MNLEQLINVLGLLVWIAARAVSRVGPHGSGLVYRELHDFYGYLQLDLLGPVVAGNRSVRTWREQADRARGTFVRRSGLNTSHILPVGGLSGGSGTLPAGLYRPEEEVFLLLNRCHGPLSTPKSACLAEVGVANASFLSRFNVGDFHGASWENGTAPDGEPGVC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F5HEN7</accession>
    <name>SCP_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Small capsomere-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SCP</name>
        <name type="ordered locus">UL48A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="J. Virol." volume="70" first="5680" last="5683">
            <title>Human cytomegalovirus (HCMV) smallest capsid protein identified as product of short open reading frame located between HCMV UL48 and UL49.</title>
            <authorList>
                <person name="Gibson W."/>
                <person name="Clopper K.S."/>
                <person name="Britt W.J."/>
                <person name="Baxter M.K."/>
            </authorList>
            <dbReference type="PubMed" id="8764088"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="J. Virol." volume="75" first="1450" last="1458">
            <title>Genetic evidence of an essential role for cytomegalovirus small capsid protein in viral growth.</title>
            <authorList>
                <person name="Borst E.M."/>
                <person name="Mathys S."/>
                <person name="Wagner M."/>
                <person name="Muranyi W."/>
                <person name="Messerle M."/>
            </authorList>
            <dbReference type="PubMed" id="11152518"/>
            <dbReference type="DOI" id="10.1128/JVI.75.3.1450-1458.2001"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3 4">Participates in the assembly of the infectious particles by decorating the outer surface of the capsid shell and thus forming a layer between the capsid and the tegument. Complexes composed of the capsid protein VP5 and UL48A assemble together in the host cytoplasm and are translocated to the nucleus, where they accumulate and participate in capsid assembly.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Participates in the assembly of the infectious particles by decorating the outer surface of the capsid shell and thus forming a layer between the capsid and the tegument. Complexes composed of the major capsid protein and small capsomere-interacting protein/SCP assemble together in the host cytoplasm and are translocated to the nucleus, where they accumulate and participate in capsid assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with the major capsid protein/MCP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 3 4">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae small capsomere-interacting protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081507.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HEN7"/>
    <dbReference type="PRIDE" id="F5HEN7"/>
    <dbReference type="DNASU" id="3077541"/>
    <dbReference type="GeneID" id="3077541"/>
    <dbReference type="KEGG" id="vg:3077541"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04021">
        <property type="entry name" value="HSV_SCP_betahv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031385">
        <property type="entry name" value="HV_small_capsid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17086">
        <property type="entry name" value="HV_small_capsid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000418247" description="Small capsomere-interacting protein">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P10219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="11152518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="8764088"/>
        </source>
    </evidence>
    <sequence length="75" mass="8495" checksum="318369AADE521528" modified="2011-06-28" version="1">MSNTAPGPTVANKRDEKHRHVVNVVLELPTEISEATHPVLATMLSKYTRMSSLFNDKCAFKLDLLRMIAVSRTRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F5HET4</accession>
    <name>U131A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL131A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL131A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="J. Gen. Virol." volume="87" first="2451" last="2460">
            <title>Role of human cytomegalovirus UL131A in cell type-specific virus entry and release.</title>
            <authorList>
                <person name="Adler B."/>
                <person name="Scrivano L."/>
                <person name="Ruzcics Z."/>
                <person name="Rupp B."/>
                <person name="Sinzger C."/>
                <person name="Koszinowski U."/>
            </authorList>
            <dbReference type="PubMed" id="16894182"/>
            <dbReference type="DOI" id="10.1099/vir.0.81921-0"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>INTERACTION WITH GH</scope>
        <source>
            <strain>AD169 with UL131A ORF from isolate VR1814</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="J. Virol." volume="82" first="60" last="70">
            <title>Characterization of the human cytomegalovirus gH/gL/UL128-131 complex that mediates entry into epithelial and endothelial cells.</title>
            <authorList>
                <person name="Ryckman B.J."/>
                <person name="Rainish B.L."/>
                <person name="Chase M.C."/>
                <person name="Borton J.A."/>
                <person name="Nelson J.A."/>
                <person name="Jarvis M.A."/>
                <person name="Johnson D.C."/>
            </authorList>
            <dbReference type="PubMed" id="17942555"/>
            <dbReference type="DOI" id="10.1128/JVI.01910-07"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH GH; GL; UL128 AND UL131A</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2012" name="J. Virol." volume="86" first="504" last="512">
            <title>Mutational mapping of pUL131A of human cytomegalovirus emphasizes its central role for endothelial cell tropism.</title>
            <authorList>
                <person name="Schuessler A."/>
                <person name="Sampaio K.L."/>
                <person name="Straschewski S."/>
                <person name="Sinzger C."/>
            </authorList>
            <dbReference type="PubMed" id="22031943"/>
            <dbReference type="DOI" id="10.1128/JVI.05354-11"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>TB40-BAC4</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2013" name="PLoS Pathog." volume="9" first="E1003463" last="E1003463">
            <title>The HCMV gH/gL/UL128-131 complex triggers the specific cellular activation required for efficient viral internalization into target monocytes.</title>
            <authorList>
                <person name="Nogalski M.T."/>
                <person name="Chan G.C."/>
                <person name="Stevenson E.V."/>
                <person name="Collins-McMillen D.K."/>
                <person name="Yurochko A.D."/>
            </authorList>
            <dbReference type="PubMed" id="23853586"/>
            <dbReference type="DOI" id="10.1371/journal.ppat.1003463"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2013" name="J. Virol." volume="87" first="9680" last="9690">
            <title>Comparative analysis of gO isoforms reveals that strains of human cytomegalovirus differ in the ratio of gH/gL/gO and gH/gL/UL128-131 in the virion envelope.</title>
            <authorList>
                <person name="Zhou M."/>
                <person name="Yu Q."/>
                <person name="Wechsler A."/>
                <person name="Ryckman B.J."/>
            </authorList>
            <dbReference type="PubMed" id="23804643"/>
            <dbReference type="DOI" id="10.1128/JVI.01167-13"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2018" name="Cell" volume="0" first="0" last="0">
            <title>An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.</title>
            <authorList>
                <person name="Martinez-Martin N."/>
                <person name="Marcandalli J."/>
                <person name="Huang C.S."/>
                <person name="Arthur C.P."/>
                <person name="Perotti M."/>
                <person name="Foglierini M."/>
                <person name="Ho H."/>
                <person name="Dosey A.M."/>
                <person name="Shriver S."/>
                <person name="Payandeh J."/>
                <person name="Leitner A."/>
                <person name="Lanzavecchia A."/>
                <person name="Perez L."/>
                <person name="Ciferri C."/>
            </authorList>
            <dbReference type="PubMed" id="30057110"/>
            <dbReference type="DOI" id="10.1016/j.cell.2018.06.028"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HOST NRP2</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2017" name="Sci. Immunol." volume="2" first="0" last="0">
            <title>Structural basis for potent antibody-mediated neutralization of human cytomegalovirus.</title>
            <authorList>
                <person name="Chandramouli S."/>
                <person name="Malito E."/>
                <person name="Nguyen T."/>
                <person name="Luisi K."/>
                <person name="Donnarumma D."/>
                <person name="Xing Y."/>
                <person name="Norais N."/>
                <person name="Yu D."/>
                <person name="Carfi A."/>
            </authorList>
            <dbReference type="PubMed" id="28783665"/>
            <dbReference type="DOI" id="10.1126/sciimmunol.aan1457"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.02 ANGSTROMS) OF 1-129</scope>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH GH; GL; UL128 AND UL130</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3 4 6 7 8">Plays a role in viral entry into host cells. Forms a pentameric complex at the surface of the viral envelope together with gH, gL, UL130 and UL131. This complex is required for entry in epithelial, endothelial and myeloid host cells (PubMed:23853586, PubMed:17942555). Mechanistically, engages host receptor(s) including neurophilin 2/NRP2 to mediate infection (PubMed:30057110). Contributes to the formation of the complex between UL128, UL130 and gH-gL.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3 7 8">Forms the envelope pentamer complex (PC) composed of gH, gL, UL128, UL130, and UL131A. The pentamer interacts with host NRP2 (PubMed:17942555, PubMed:28783665, PubMed:30057110). The interaction with gH is important for the formation of UL128, UL130, gH-gL complex (PubMed:16894182).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Virion membrane</location>
        </subcellularLocation>
        <text evidence="5">Found as a pentameric complex at the surface of virion envelope.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081566.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PDB" id="5VOB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.02 A"/>
        <property type="chains" value="E=1-129"/>
    </dbReference>
    <dbReference type="PDB" id="5VOC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.99 A"/>
        <property type="chains" value="E=1-129"/>
    </dbReference>
    <dbReference type="PDB" id="5VOD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="5.90 A"/>
        <property type="chains" value="E=1-129"/>
    </dbReference>
    <dbReference type="PDB" id="7KBB">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.02 A"/>
        <property type="chains" value="E=19-129"/>
    </dbReference>
    <dbReference type="PDB" id="7M22">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.65 A"/>
        <property type="chains" value="E=19-129"/>
    </dbReference>
    <dbReference type="PDB" id="7M30">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.81 A"/>
        <property type="chains" value="E=19-129"/>
    </dbReference>
    <dbReference type="PDBsum" id="5VOB"/>
    <dbReference type="PDBsum" id="5VOC"/>
    <dbReference type="PDBsum" id="5VOD"/>
    <dbReference type="PDBsum" id="7KBB"/>
    <dbReference type="PDBsum" id="7M22"/>
    <dbReference type="PDBsum" id="7M30"/>
    <dbReference type="SMR" id="F5HET4"/>
    <dbReference type="PRIDE" id="F5HET4"/>
    <dbReference type="ABCD" id="F5HET4">
        <property type="antibodies" value="1 sequenced antibody"/>
    </dbReference>
    <dbReference type="GeneID" id="3077493"/>
    <dbReference type="KEGG" id="vg:3077493"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019031">
        <property type="term" value="C:viral envelope"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055036">
        <property type="term" value="C:virion membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098670">
        <property type="term" value="P:entry receptor-mediated virion attachment to host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046718">
        <property type="term" value="P:viral entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-1161">Viral attachment to host cell</keyword>
    <keyword id="KW-1234">Viral attachment to host entry receptor</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418315" description="Protein UL131A">
        <location>
            <begin position="19"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="22"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="36"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="74"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="106"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16894182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="17942555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="22031943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="23804643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="23853586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="28783665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="8">
        <source>
            <dbReference type="PubMed" id="30057110"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="5VOB"/>
        </source>
    </evidence>
    <sequence length="129" mass="14989" checksum="3488C28116DF2D56" modified="2011-06-28" version="1" precursor="true">MRLCRVWLSVCLCAVVLGQCQRETAEKNDYYRVPHYWDACSRALPDQTRYKYVEQLVDLTLNYHYDASHGLDNFDVLKRINVTEVSLLISDFRRQNRRGGTNKRTTFNAAGSLAPHARSLEFSVRLFAN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F5HEU0</accession>
    <name>US03_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane glycoprotein US3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US3</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text>Retains, but does not degrade MHC class I heterodimers in the endoplasmic reticulum during the immediate-early period of virus infection, thereby impairing their transport and maturation. Forms a complex with beta-2-microglobulin-associated class I heavy chains, which accumulate in the ER. In consequence, infected cells are masked for immune recognition by cytotoxic T-lymphocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="developmental stage">
        <text>Expressed at immediate-early period of virus infection and at reduced levels at early-late times.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">The signal sequence is not cleaved.</text>
    </comment>
    <comment type="PTM">
        <text>N-glycosylated; mostly exists in a high-mannose form.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytomegalovirus US2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081590.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HEU0"/>
    <dbReference type="BioGRID" id="1678085">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HEU0"/>
    <dbReference type="DNASU" id="3077532"/>
    <dbReference type="GeneID" id="3077532"/>
    <dbReference type="KEGG" id="vg:3077532"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044167">
        <property type="term" value="C:host cell endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009237">
        <property type="entry name" value="Herpes_US2/US3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05963">
        <property type="entry name" value="Cytomega_US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0393">Immunoglobulin domain</keyword>
    <keyword id="KW-0430">Lectin</keyword>
    <keyword id="KW-0465">Mannose-binding</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416721" description="Membrane glycoprotein US3">
        <location>
            <begin position="21"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="21"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="182"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="44"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="186" mass="21556" checksum="34D58C17BE5EA06B" modified="2011-06-28" version="1" precursor="true">MKPVLVLAILAVLFLRLADSVPRPLNVVVSEIKSAHFRVEENQCWFHMGMLYFKGRMSGNFTKKHFVNVGIVSQSYMDRLQVSGEQYHHDERGAYFEWNIGGYPVSHTVDMVDITLSTRWGDPKKYAACVPQVRMDYSSQTINWYLQRSMRDDNWGLLFRTLLVYLFSLVVLVLLTVGVSARLRFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>F5HF23</accession>
    <name>RL5A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein RL5A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RL5A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081456.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HF23"/>
    <dbReference type="PRIDE" id="F5HF23"/>
    <dbReference type="DNASU" id="3077469"/>
    <dbReference type="GeneID" id="3077469"/>
    <dbReference type="KEGG" id="vg:3077469"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024349">
        <property type="entry name" value="HHV5_RL5A/RL6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11088">
        <property type="entry name" value="RL11D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418235" description="Protein RL5A">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <sequence length="95" mass="10650" checksum="397CF07F42BC3195" modified="2011-06-28" version="1">MDMRKLNTSQGRNLTTVDDRRRFSIGWETWDDGGESLYDVTNNGTTVINTTACVSSCSHTSLVLCNMTQQTDSLYGVGHRLNDEEDGELWRVSVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>F5HF44</accession>
    <name>UL145_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL145</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL145</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081572.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HF44"/>
    <dbReference type="DNASU" id="3077415"/>
    <dbReference type="GeneID" id="3077415"/>
    <dbReference type="KEGG" id="vg:3077415"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418329" description="Protein UL145">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <sequence length="130" mass="14625" checksum="3AF79CD700AB1C0E" modified="2011-06-28" version="1">MYGVLAHYYSFISSPSVMVNFKHHNAVQLLCARTRDGTAGWERLTHHASYHANYGAYAVLMATSQRKSLVLHRYSAVTAVALQLMPVEMLRRLDQSDWVRGAWIVSETFPTSDPKGFWSDDDSPMGGSED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F5HF49</accession>
    <name>AN_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alkaline nuclease</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL98</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in processing non linear or branched viral DNA intermediates in order to promote the production of mature packaged unit-length linear progeny viral DNA molecules. Exhibits endonuclease and exonuclease activities and accepts both double-stranded and single-stranded DNA as substrate. Exonuclease digestion of DNA is in the 5'-&gt; 3' direction and the products are 5'-monophosphate nucleosides. Additionally, forms a recombinase with the major DNA-binding protein, which displays strand exchange activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with major DNA-binding protein; this interaction increases the nuclease processivity of the alkaline exonuclease.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae alkaline nuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081545.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HF49"/>
    <dbReference type="PRIDE" id="F5HF49"/>
    <dbReference type="GeneID" id="3077527"/>
    <dbReference type="KEGG" id="vg:3077527"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04009">
        <property type="entry name" value="HSV_AN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001616">
        <property type="entry name" value="Herpes_alk_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034720">
        <property type="entry name" value="Viral_alk_exo"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01771">
        <property type="entry name" value="Viral_alk_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00924">
        <property type="entry name" value="ALKEXNUCLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417829" description="Alkaline nuclease">
        <location>
            <begin position="1"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="site" description="Required for function" evidence="1">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="site" description="Required for function" evidence="1">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="site" description="Required for function" evidence="1">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="site" description="Required for function" evidence="1">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="584" mass="65313" checksum="3B5A571DA4253118" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5HF90</accession>
    <name>UL22A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Glycoprotein UL22A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL22A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HHV-5 UL22A protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081481.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HF90"/>
    <dbReference type="GeneID" id="3077571"/>
    <dbReference type="KEGG" id="vg:3077571"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009245">
        <property type="entry name" value="Cytomegalo_UL22A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05984">
        <property type="entry name" value="Cytomega_UL20A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416714" description="Glycoprotein UL22A">
        <location>
            <begin position="21"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="21"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="105" mass="11304" checksum="3D4A80E37251B6D2" modified="2011-06-28" version="1" precursor="true">MARRLWILSLLAVTLTVALAAPSQKSKRSVTVEQPSTSTNSDGNTTPSKNVTLSQGGSTTDGDEDYSGGDYDVLITDTDGGNHQQPQEKTDEHKGEHTKENEKTQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F5HFA5</accession>
    <name>UL117_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL117</fullName>
            <shortName>pUL117</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL117</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the inhibition of host DNA replication in the infected cell. Targets the mini-chromosome maintenance (MCM) complex and blocks the accumulation of MCM proteins and their loading onto host chromatin.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Isoform UL117</molecule>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <text evidence="1">The major fraction localizes to nuclear compartments.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Isoform UL117.5</molecule>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="alternative initiation"/>
        <isoform>
            <id>F5HFA5-1</id>
            <name>UL117</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>F5HFA5-2</id>
            <name>UL117.5</name>
            <sequence type="described" ref="VSP_044020"/>
        </isoform>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae U84 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081557.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HFA5"/>
    <dbReference type="DNASU" id="3077511"/>
    <dbReference type="GeneID" id="3077511"/>
    <dbReference type="KEGG" id="vg:3077511"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005028">
        <property type="entry name" value="Herpes_IE2_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03361">
        <property type="entry name" value="Herpes_IE2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0024">Alternative initiation</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418276" description="Protein UL117">
        <location>
            <begin position="1"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_044020" description="In isoform UL117.5." evidence="3">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="425" mass="45592" checksum="3E235734E02C9401" modified="2011-06-28" version="1">MVMFSQDHVQIVYGSTRICKSLAPANKRKTHRTIVVAPRRGFLRIPPDGQDVNHVKIVPTTTSSSLAPPRDDERRPTPPLRPPLTVYPYGTSLIRRSARDAKLRSKLIVFHITRPALGQHPQNPGISGPAAMDHSEFLTSFRREVDRQTVLTAESAPATVEVCLGDALPGGVMGGGGLPAGVGSASAAVAAAAAAVAGVPVAANPVMPATATVTTPPMIDLTSHHRPLTLFTPASAAAAPAVATNGGNATYILPADCRYAPLFASKYKYVFEEVSRLMRLHDSTAVQLQISASCGNAFQALKSALLKLHNVTVLAGQQLITQTMPHTPQAVATFKFFHQDPNRVLDCIRPVVPRSTSYHETGVYQMWVSGATKKDLFDAVTLCASIVEKQPDVFNINVSLLTYPSIAAPHLPLYNEFTSFRLPTS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F5HFB4</accession>
    <name>UL18_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane glycoprotein UL18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL18</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the protection against host NK cell cytotoxicity by interacting with and modulating the activity of the host inhibitory leukocyte Ig-like receptor 1/LILRB1, which is expressed on monocytes, dendritic cells, as well as subsets of T and NK cells. UL18 exerts an inhibitory effect on LIR-1+ NK cells, while it stimulates LIR-1- NK cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host LILRB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081477.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HFB4"/>
    <dbReference type="PRIDE" id="F5HFB4"/>
    <dbReference type="DNASU" id="3077466"/>
    <dbReference type="GeneID" id="3077466"/>
    <dbReference type="KEGG" id="vg:3077466"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="SSF48726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="SSF54452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0391">Immunity</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0490">MHC I</keyword>
    <keyword id="KW-1131">Modulation of host NK-cell activity by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418297" description="Membrane glycoprotein UL18">
        <location>
            <begin position="20"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="368" mass="41730" checksum="3E814AE4A8033A12" modified="2011-06-28" version="1" precursor="true">MMTMWCLTLFVLWMLRVVGMHVLRYGYTGIFDDTSHMTLTVVGIFDGQHFFTYHVNSSDKASSRANGTISWMANVSAAYPTYLDGERAKGDLIFNQTEQNLLELEIALGYRSQSVLTWTHECNTTENGSFVAGYEGFGWDGETLMELKDNLTLWTGPNYEISWLKQNKTYIDGKIKNISEGDTTIQRNYLKGNCTQWSVIYSGFQTPVTHPVVKGGVRNQNDNRAEAFCTSYGFFPGEINITFIHYGNKAPDDSEPQCNPLLPTFDGTFHQGCYVAIFCNQNYTCRVTHGNWTVEIPISVTSPDDSSSGEVPDHPTANKRYNTMTISSVLLALLLCALLFAFLHYFTTLKQYLRNLAFAWRYRKVRSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5HFG3</accession>
    <name>UL41A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL41A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL41A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 UL41A protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081499.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HFG3"/>
    <dbReference type="DNASU" id="3077475"/>
    <dbReference type="GeneID" id="3077475"/>
    <dbReference type="KEGG" id="vg:3077475"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020141">
        <property type="entry name" value="Herpesvirus_UL41A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17591">
        <property type="entry name" value="UL41A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000418286" description="Protein UL41A">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="78" mass="8953" checksum="41815D53118D7C4F" modified="2011-06-28" version="1">MTLFCRTANSTAGYVDMNVCIGMIGVVCFVFGVFILIFCSTLKAFYVRQKTYHLLGTESDDEETTVWEKRRMESDTDF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-02-23" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F5HFZ4</accession>
    <name>NEC1_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nuclear egress protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NEC1</name>
        <name type="ordered locus">UL53</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2015" name="J. Biol. Chem." volume="290" first="27452" last="27458">
            <title>Crystal Structure of the Human Cytomegalovirus pUL50-pUL53 Core Nuclear Egress Complex Provides Insight into a Unique Assembly Scaffold for Virus-Host Protein Interactions.</title>
            <authorList>
                <person name="Walzer S.A."/>
                <person name="Egerer-Sieber C."/>
                <person name="Sticht H."/>
                <person name="Sevvana M."/>
                <person name="Hohl K."/>
                <person name="Milbradt J."/>
                <person name="Muller Y.A."/>
                <person name="Marschall M."/>
            </authorList>
            <dbReference type="PubMed" id="26432641"/>
            <dbReference type="DOI" id="10.1074/jbc.c115.686527"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.44 ANGSTROMS) OF 1-175</scope>
        <scope>INTERACTION WITH NEC2</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Plays an essential role in virion nuclear egress, the first step of virion release from infected cell. Within the host nucleus, NEC1 interacts with the newly formed capsid through the vertexes and directs it to the inner nuclear membrane by associating with NEC2. Induces the budding of the capsid at the inner nuclear membrane as well as its envelopment into the perinuclear space. There, the NEC1/NEC2 complex promotes the fusion of the enveloped capsid with the outer nuclear membrane and the subsequent release of the viral capsid into the cytoplasm where it will reach the secondary budding sites in the host Golgi or trans-Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Forms a heterohexameric complex with NEC2. Interacts with capsid vertex specific component 2/CVC2; this interaction directs the capsid to the host inner nuclear membrane to initiate budding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host nucleus inner membrane</location>
        </subcellularLocation>
        <text evidence="2">Remains attached to the nucleus inner membrane through interaction with NEC2.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1 2">Phosphorylated at serine residues in the N-terminus. This phosphorylation regulates the localization within the inner nuclear membrane (By similarity). Phosphorylation by viral kinase UL97 at Ser-19 plays an important role for correct viral nuclear egress complex (NEC) localization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the herpesviridae NEC1 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081512.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PDB" id="5D5N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.44 A"/>
        <property type="chains" value="B=50-292"/>
    </dbReference>
    <dbReference type="PDBsum" id="5D5N"/>
    <dbReference type="SMR" id="F5HFZ4"/>
    <dbReference type="PRIDE" id="F5HFZ4"/>
    <dbReference type="DNASU" id="3077575"/>
    <dbReference type="GeneID" id="3077575"/>
    <dbReference type="KEGG" id="vg:3077575"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04023">
        <property type="entry name" value="HSV_NEC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021152">
        <property type="entry name" value="Herpes_UL31"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02718">
        <property type="entry name" value="Herpes_UL31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000416452" description="Nuclear egress protein 1">
        <location>
            <begin position="1"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CCCH-type" evidence="2">
        <location>
            <begin position="106"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="316"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="360"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="94"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="105"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="113"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="119"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="123"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="134"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="144"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="152"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="157"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="162"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="188"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="224"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="228"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="238"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="271"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="281"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="26432641"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="5">
        <source>
            <dbReference type="PDB" id="5D5N"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="5D5N"/>
        </source>
    </evidence>
    <sequence length="376" mass="42302" checksum="07A2E48A86AFE140" modified="2011-06-28" version="1">MSSVSGVRTPRERRSALRSLLRKRRQRELASKVASTVNGATSANNHGEPPSPADARPRLTLHDLHDIFREHPELELKYLNMMKMAITGKESICLPFNFHSHRQHTCLDISPYGNEQVSRIACTSCEDNRILPTASDAMVAFINQTSNIMKNRNFYYGFCKSSELLKLSTNQPPIFQIYYLLHAANHDIVPFMHAENGRLHMHVIFENSDVHIPCDCITQMLTAAREDYSVTLNIVRDHVVISVLCHAVSASSVKIDVTILQRKIDEMDIPNDVSESFERYKELIQELCQSSGNNLYEEATSSYAIRSPLTASPLHVVSTNGCGPSSSSQSTPPHLHPPSQATQPHHYSHHQSQSQQHHHRPQSPPPPLFLNSIRAP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>F5HGC2</accession>
    <name>UL30_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL30</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HHV-5 UL30 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081489.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HGC2"/>
    <dbReference type="DNASU" id="3077470"/>
    <dbReference type="GeneID" id="3077470"/>
    <dbReference type="KEGG" id="vg:3077470"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418282" description="Uncharacterized protein UL30">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="121" mass="13986" checksum="54A0E6063005A6E4" modified="2011-06-28" version="1">MTTSAMTAPDTRRQLQHVETLRRFLRGDSCFVHDLRGMMDYHDGLSRRQQRAFCRAGRVLTDPEPIQSETEGENKQFTEHTHKVVSFFIKSVFVFPCLVLPNCCQVSVDRSRVPETGGRWL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F5HGH8</accession>
    <name>US20_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane protein US20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US20</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081605.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HGH8"/>
    <dbReference type="PRIDE" id="F5HGH8"/>
    <dbReference type="DNASU" id="3077561"/>
    <dbReference type="GeneID" id="3077561"/>
    <dbReference type="KEGG" id="vg:3077561"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418322" description="Membrane protein US20">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="254" mass="28531" checksum="58E714AFDF58B620" modified="2011-06-28" version="1">MQAQEANALLLSRMEALEWFKKFTVWLRVYAIFIFQLAFSFGLGSVFWLGFPQNRNFCVENYSFFLTVLVPIVCMFITYTLGNEHPSNATVLFIYLLANSLTAAIFQMCSESRVLVGSYVMTLALFISFTGLAFLGGRDRRRWKCISCVYVVMLLSFLTLALLSDADWLQKIVVTLCAFSISFFLGILAYDSLMVIFFCPPNQCIRHAVCLYLDSMAIFLTLLLMLSGPRWISLSDGAPLDNGTLTAASTTGKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5HGJ4</accession>
    <name>PP85_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoprotein 85</fullName>
            <shortName>pp85</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Phosphoprotein UL25</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">UL25</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Expressed exclusively at the cytoplasmic level during the late phase of the virus replication cycle. Also found in dense bodies (By similarity).</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">Phosphorylated.</text>
    </comment>
    <comment type="miscellaneous">
        <text>Major target antigen for the anti-CMV antibody response.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae pp85 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081484.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HGJ4"/>
    <dbReference type="PRIDE" id="F5HGJ4"/>
    <dbReference type="DNASU" id="3077473"/>
    <dbReference type="GeneID" id="3077473"/>
    <dbReference type="KEGG" id="vg:3077473"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006731">
        <property type="entry name" value="Herpes_pp85"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04637">
        <property type="entry name" value="Herpes_pp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="SSF47473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000418310" description="Phosphoprotein 85">
        <location>
            <begin position="1"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="615"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="634"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="656" mass="73539" checksum="5A1AB5530FCA0BF8" modified="2011-06-28" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F5HGP1</accession>
    <name>GO_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Envelope glycoprotein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL74</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="PLoS Pathog." volume="13" first="E1006281" last="E1006281">
            <title>Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-alpha as a key for entry.</title>
            <authorList>
                <person name="Wu Y."/>
                <person name="Prager A."/>
                <person name="Boos S."/>
                <person name="Resch M."/>
                <person name="Brizic I."/>
                <person name="Mach M."/>
                <person name="Wildner S."/>
                <person name="Scrivano L."/>
                <person name="Adler B."/>
            </authorList>
            <dbReference type="PubMed" id="28403202"/>
            <dbReference type="DOI" id="10.1371/journal.ppat.1006281"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH GL; GH AND HOST PDGFRA</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Plays a role in viral entry into host cells. Forms a trimeric complex at the surface of the viral envelope together with gH and gL. This complex is required for entry in host fibroblasts (PubMed:28403202). Mechanistically, engages host receptor(s) including PDGFRA to mediate infection (PubMed:28403202).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms the envelope trimer complex composed of gH, gL, and gO. The trimer interacts with host PDGFRA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Virion membrane</location>
        </subcellularLocation>
        <text evidence="3">Host membrane associated, either via its interaction with gH, or as a type II transmembrane protein.</text>
    </comment>
    <comment type="PTM">
        <text>N-glycosylated.</text>
    </comment>
    <comment type="PTM">
        <text>The N-terminus is blocked.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae U47 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081522.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HGP1"/>
    <dbReference type="PRIDE" id="F5HGP1"/>
    <dbReference type="DNASU" id="3077572"/>
    <dbReference type="GeneID" id="3077572"/>
    <dbReference type="KEGG" id="vg:3077572"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012564">
        <property type="entry name" value="Herpes_UL74"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07982">
        <property type="entry name" value="Herpes_UL74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418232" description="Envelope glycoprotein O">
        <location>
            <begin position="32"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="398"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="439"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="28403202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="472" mass="54701" checksum="5C6291E5CC9F65E0" modified="2011-06-28" version="1" precursor="true">MGKKEMIMVKGIPKIMLLISITFLLLSLINCNVLVNSRGTRRSWPYTVLSYRGKEILKKQKEDILKRLMSTSSDGYRFLMYPSQQKFHAIVISMDKFPQDYILAGPIRNDSITHMWFDFYSTQLRKPAKYVYSEYNHTAHKITLRPPPCGTVPSMNCLSEMLNVSKRNDTGEKGCGNFTTFNPMFFNVPRWNTKLYIGSNKVNVDSQTIYFLGLTALLLRYAQRNCTRSFYLVNAMSRNLFRVPKYINGTKLKNTMRKLKRKQALVKEQPQKKNKKSQSTTTPYLSYTTSTAFNVTTNVTYSATAAVTRVATSTTGYRPDSNFMKSIMATQLRDLATWVYTTLRYRNEPFCKPDRNRTAVSEFMKNTHVLIRNETPYTIYGTLDMSSLYYNETMSVENETASDNNETTPTSPSTRFQRTFIDPLWDYLDSLLFLDKIRNFSLQLPAYGNLTPPEHRRAANLSTLNSLWWWSQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5HGU6</accession>
    <name>UL132_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Envelope glycoprotein UL132</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL132</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Virion membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 UL132 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081567.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HGU6"/>
    <dbReference type="GeneID" id="3077441"/>
    <dbReference type="KEGG" id="vg:3077441"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019031">
        <property type="term" value="C:viral envelope"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055036">
        <property type="term" value="C:virion membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021023">
        <property type="entry name" value="UL132"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11359">
        <property type="entry name" value="gpUL132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418243" description="Envelope glycoprotein UL132">
        <location>
            <begin position="28"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="270" mass="29782" checksum="6003D648D3BA2BD4" modified="2011-06-28" version="1" precursor="true">MPAPRGPLRATFLALVAFGLLLQIDLSDVTNVTSSTKVPTSTSNRNSVDNATSSGPTTGINMTTTHESSVHNVRNNEIMKVLAILFYIVTGTSIFSFIAVLVAVVYSSCCKHPGRFRFADEEAVNLLDDTDDSGGSSPFGSGSRRGSQIPAGFCSSSPYQRLETRDWDEEEEASAARERMKHDPENVIYFRKDGNLDTSFVNPNYGRGSPLTIESHLSDNEEDPIRYYVSVYDELTASEMEEPSNSTSWQIPKLMKVAMQPVSLRDPEYD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F5HHH2</accession>
    <name>UL142_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane glycoprotein UL142</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL142</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Immunol." volume="175" first="7457" last="7465">
            <title>Human cytomegalovirus encodes an MHC class I-like molecule (UL142) that functions to inhibit NK cell lysis.</title>
            <authorList>
                <person name="Wills M.R."/>
                <person name="Ashiru O."/>
                <person name="Reeves M.B."/>
                <person name="Okecha G."/>
                <person name="Trowsdale J."/>
                <person name="Tomasec P."/>
                <person name="Wilkinson G.W."/>
                <person name="Sinclair J."/>
                <person name="Sissons J.G."/>
            </authorList>
            <dbReference type="PubMed" id="16301653"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="J. Virol." volume="83" first="12345" last="12354">
            <title>NKG2D ligand MICA is retained in the cis-Golgi apparatus by human cytomegalovirus protein UL142.</title>
            <authorList>
                <person name="Ashiru O."/>
                <person name="Bennett N.J."/>
                <person name="Boyle L.H."/>
                <person name="Thomas M."/>
                <person name="Trowsdale J."/>
                <person name="Wills M.R."/>
            </authorList>
            <dbReference type="PubMed" id="19793804"/>
            <dbReference type="DOI" id="10.1128/JVI.01175-09"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HOST MICA</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2010" name="J. Immunol." volume="185" first="1093" last="1102">
            <title>Intracellular sequestration of the NKG2D ligand ULBP3 by human cytomegalovirus.</title>
            <authorList>
                <person name="Bennett N.J."/>
                <person name="Ashiru O."/>
                <person name="Morgan F.J."/>
                <person name="Pang Y."/>
                <person name="Okecha G."/>
                <person name="Eagle R.A."/>
                <person name="Trowsdale J."/>
                <person name="Sissons J.G."/>
                <person name="Wills M.R."/>
            </authorList>
            <dbReference type="PubMed" id="20530255"/>
            <dbReference type="DOI" id="10.4049/jimmunol.1000789"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HOST ULBP3</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3 4">Participates in the inhibition of the host immune response. Prevents host NK cell-mediated lysis of the infected cell by preventing the KLRK1 ligand 3/ULBP3 trafficking to the cell surface. Retains also another KLRK1 ligand, MHC class I-related chain A/MICA, in the Golgi apparatus to avoid its surface expression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">Interacts with host MICA and ULBP3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Host Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081574.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HHH2"/>
    <dbReference type="DNASU" id="3077552"/>
    <dbReference type="GeneID" id="3077552"/>
    <dbReference type="KEGG" id="vg:3077552"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="SSF54452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418303" description="Membrane glycoprotein UL142">
        <location>
            <begin position="20"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="1">
        <location>
            <begin position="20"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="289"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16301653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="19793804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="20530255"/>
        </source>
    </evidence>
    <sequence length="305" mass="34626" checksum="6E67EFD0E22B8F5A" modified="2011-06-28" version="1" precursor="true">MRIEWACWLFGYFVSSVGSERSLSYRYHLESNSSANVVCNGNISVFVNGTLGVRYNITVGISSSLLIGHLTIQTLESWFTPWVQNKSYSKQPLSTTETLYNIDSENIHRVSQYFHTRWIKSLQENHTCDLTNSTPTYTYQANVNNTNYLTLTSSGWQDRLNYTAINSTHFNLTESNITSIHKYLNTTCIERLRNYTLEPVYTTAVPQNVTPEHAITTLYTTPPNAITIKDTTQSHTVQTPSFNDTHNVTEHTLNISYVLSQKTNNTTSPWVYAIPMGATATIGAGLYIGKHFTPVKFVYEVWRGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F5HHS3</accession>
    <name>UL124_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL124</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL124</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 UL124 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081563.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HHS3"/>
    <dbReference type="DNASU" id="3077503"/>
    <dbReference type="GeneID" id="3077503"/>
    <dbReference type="KEGG" id="vg:3077503"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020214">
        <property type="entry name" value="Cytomega_UL124"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17609">
        <property type="entry name" value="HCMV_UL124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418278" description="Uncharacterized protein UL124">
        <location>
            <begin position="21"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="150" mass="15628" checksum="745C8678A56FDEEF" modified="2011-06-28" version="1" precursor="true">MERNSLLVCQLLCLVARAAATSTAQTTLPSTVNSTATGVTSDSQQNTTQLPASSAAALSLPSASAVQAHSPSSFSDTYPTATALCGTLVVVGIVLCLSLASTVRSKELPSDHEPLEAWEQGSDVEAPPLPEKSPCPEHVPEIRVEIPRYV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F5HHZ3</accession>
    <name>UL42_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL42</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2016" name="J. Gen. Virol." volume="97" first="196" last="208">
            <title>Degradation of host ubiquitin E3 ligase Itch by human cytomegalovirus UL42.</title>
            <authorList>
                <person name="Koshizuka T."/>
                <person name="Tanaka K."/>
                <person name="Suzutani T."/>
            </authorList>
            <dbReference type="PubMed" id="26555021"/>
            <dbReference type="DOI" id="10.1099/jgv.0.000336"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HOST ITCH</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Plays a role in the inhibition of host innate immune response to promote latent infection. Mechanistically, suppresses viral DNA-triggered signaling by impairing DNA binding and oligomerization of CGAS. Impairs also the translocation of host STING1 from the endoplasmic reticulum to perinuclear punctate structures which is an essential step for its activation (By similarity). Regulates the function of host NEDD4 family ubiquitin E3 ligases through its PPxY motif and thereby prevents the excessive ubiquitination of gB and its degradation by inhibiting these E3 ligases.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Interacts with host ITCH; this interaction induces the ubiquitination and subsequent degradation of ITCH (By similarity). Interacts with host STING1. Interacts with CGAS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Host membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Host cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">Accumulates in the perinuclear region of the host cytoplasm, with some dispersal into the cytoplasm in a fine-speckled pattern.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Contains one L domain: a PPXY motif which is involved in the interaction with ITCH, a member of the NEDD4 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Cytomegalovirus UL42 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081500.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HHZ3"/>
    <dbReference type="BioGRID" id="1677993">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HHZ3"/>
    <dbReference type="DNASU" id="3077440"/>
    <dbReference type="GeneID" id="3077440"/>
    <dbReference type="KEGG" id="vg:3077440"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030430">
        <property type="term" value="C:host cell cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033644">
        <property type="term" value="C:host cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039503">
        <property type="term" value="P:suppression by virus of host innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035110">
        <property type="entry name" value="UL42"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17638">
        <property type="entry name" value="UL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="chain" id="PRO_0000418316" description="Protein UL42">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="PPXY motif" evidence="1">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="PPXY motif" evidence="1">
        <location>
            <begin position="42"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="D5LX53"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P16815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="26555021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="125" mass="13668" checksum="79870B50807CCB66" modified="2011-06-28" version="1">MEPTPMLRDRDHDDAPPTYEQAMGLCPTTVSTPPPPPPDCSPPPYRPPYCLVSSPSPRHTFDMDMMEMPATMHPTTGAYFDNGWKWTFALLVVAILGIIFLAVVFTVVINRDSANITTGTQASSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-02-23" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F5HI85</accession>
    <name>UNG_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uracil-DNA glycosylase</fullName>
            <shortName evidence="1">UDG</shortName>
            <ecNumber evidence="1">3.2.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UNG</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">UL114</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or deamination of cytosines. Therefore may reduce deleterious uracil incorporation into the viral genome, particularly in terminally differentiated cells which lack DNA repair enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="1"/>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081554.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F5HI85"/>
    <dbReference type="BioGRID" id="1678092">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HI85"/>
    <dbReference type="GeneID" id="3077539"/>
    <dbReference type="KEGG" id="vg:3077539"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018085">
        <property type="entry name" value="Ura-DNA_Glyclase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="PTHR11264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00130">
        <property type="entry name" value="U_DNA_GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000416439" description="Uracil-DNA glycosylase">
        <location>
            <begin position="1"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04046"/>
        </source>
    </evidence>
    <sequence length="250" mass="28353" checksum="0CC28AC4DC155508" modified="2011-06-28" version="1">MALKQWMLANIADNKGSLLTPDEQARVFCLSADWIRFLSLPDHDTVLLRDTVAAVEGARQLEMVYPAPEHVHRWSYLCPPEQVRVVIVGQDPYCDGSASGLAFGTLAGRPPPPSLNNVFRELARTVDGFQRPASGCLDAWARRGVLLLNTVFTVVHGQPGSHRHLGWQTLSNHVIRRLSERREHLVFMLWGADAHTCEYLIDRRRHLVLKSCHPSPRNTTRAFVGNDHFILANAYLDTHYRETMDWRLCG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F5HI87</accession>
    <name>CEP3_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytoplasmic envelopment protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>28 kDa structural phosphoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>pp28</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">UL99</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the cytoplasmic envelopment of tegument proteins and capsids during the assembly and egress processes. Participates also in viral entry at the fusion step probably by regulating the core fusion machinery.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with cytoplasmic envelopment protein 2; this interaction is essential for the proper localization of each protein to the assembly complex and thus for the production of infectious virus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Virion membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host Golgi apparatus membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="1">Virion membrane-associated tegument protein. Associates with host membrane lipids rafts. During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN).</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Myristoylation and palmitoylation (probably on one or more of the nearby cysteines at the N-terminus) enable membrane-binding and Golgi apparatus-specific targeting and are essential for efficient packaging.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated. Phosphorylation does not seem to be required for recycling to the host Golgi apparatus. Packaging is selective for underphosphorylated forms.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae cytoplasmic envelopment protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081546.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F5HI87"/>
    <dbReference type="DNASU" id="3077533"/>
    <dbReference type="GeneID" id="3077533"/>
    <dbReference type="KEGG" id="vg:3077533"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04041">
        <property type="entry name" value="HSV_CEP3_betahv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034705">
        <property type="entry name" value="HSV_CEP3_betahv"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0519">Myristate</keyword>
    <keyword id="KW-0564">Palmitate</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="initiator methionine" description="Removed; by host" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418277" description="Cytoplasmic envelopment protein 3" evidence="1">
        <location>
            <begin position="2"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="14"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="N-myristoyl glycine; by host" evidence="1">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="190" mass="20923" checksum="7FBD4803A6DA4471" modified="2011-06-28" version="1">MGAELCKRICCEFGTTSGEPLKDALGRQVSLRSYDNIPPTSSSDEGEDDDDGEDDDNEERQQKLRLCGSGCGGNDSSSGSHREATHDGPKKNAVRSTFREDKAPKPSKQSKKKKKPSKHHHHQQSSIMQETDDLDEEDTSIYLSPPPVPPVQVVAKRLPRPDTPRTPRQKKISQRPPTPGTKKPAASLPF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>F7V996</accession>
    <name>RL9A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein RL9A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RL9A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AEJ33667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_004940330.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="F7V996"/>
    <dbReference type="GeneID" id="13229473"/>
    <dbReference type="KEGG" id="vg:13229473"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418308" description="Protein RL9A">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="47" mass="5270" checksum="C0EE7AFC6A3598FD" modified="2011-09-21" version="1">MSLDAASHQPAARRLLDSALVRRVLACMIIVIMIAISIWILTYVLFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>F7V998</accession>
    <name>U150A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL150A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL150A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AEJ33669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_004940331.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="F7V998"/>
    <dbReference type="GeneID" id="13229471"/>
    <dbReference type="KEGG" id="vg:13229471"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418330" description="Uncharacterized protein UL150A">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <sequence length="271" mass="29410" checksum="5F68F62B023CA27B" modified="2011-09-21" version="1">MASCSPPRRVSPDLLVSSELRRRNIWRRVTCVTGERGSPAVTTAVSSTRVRPAVLCGKAPVSETVDEKENGAATAGGGGVNAAVDVRRCEYSETAVRQKLDPSGNDSRCVVAAADSSTDSDGKSIIAGVQVVDHDEDIIAPQSLWCTAFKEALWDVALLEVPRWAWQGWKRWRNSESGRRWSAGSASASSLSDLAGEAVGELVGSVVAYVILERLWLAARGWVCETGVEAEEAMARRRQRMLWRMFSRGGDGECSRRCSMEMACEEESAVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-02-23" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SVX3</accession>
    <name>US34A_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein US34A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US34A</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HHV-5 US34A protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081618.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SVX3"/>
    <dbReference type="PRIDE" id="Q6SVX3"/>
    <dbReference type="GeneID" id="3077447"/>
    <dbReference type="KEGG" id="vg:3077447"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031384">
        <property type="entry name" value="HHV-5_US34A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17087">
        <property type="entry name" value="HHV-5_US34A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000115296" description="Uncharacterized protein US34A">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="64" mass="8085" checksum="49CEB5E5E1441DB7" modified="2004-07-05" version="1">MLKFFLKLRKRRRPVVVPRFVRFIVYVVLFTVAVQRVKQERDAHLRRYEERLQKNRARRRQSFP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SVZ0</accession>
    <accession>D2K3W1</accession>
    <name>US16_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein US16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">US16</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="J. Virol." volume="86" first="6875" last="6888">
            <title>The US16 gene of Human cytomegalovirus is required for efficient viral infection of endothelial and epithelial cells.</title>
            <authorList>
                <person name="Bronzini M."/>
                <person name="Luganini A."/>
                <person name="Dell'oste V."/>
                <person name="De Andrea M."/>
                <person name="Landolfo S."/>
                <person name="Gribaudo G."/>
            </authorList>
            <dbReference type="PubMed" id="22496217"/>
            <dbReference type="DOI" id="10.1128/JVI.06310-11"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host cytoplasm</location>
        </subcellularLocation>
        <text>Localizes in cytoplasmic assembly compartments but not present in virions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytomegalovirus US12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081601.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SVZ0"/>
    <dbReference type="DNASU" id="3077558"/>
    <dbReference type="GeneID" id="3077558"/>
    <dbReference type="KEGG" id="vg:3077558"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418321" description="Protein US16">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22496217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="309" mass="34689" checksum="DB88FE0B1BAF6B94" modified="2004-07-05" version="1">MGLRFPTATQRQIVFRRLFDSGNNDDYDEAAVVAVLGWVHRFEVVVRIAGLLLFQISTAVAVLGSFSLVFPTATLKSRPGFPCHVVWAPEVLLLVPVASALFVYFRYERPVLAQRNRHPRCRRPFRQLVLLLAGLLAHIPALGVTCACQEPREVLTSFVLTLVITLLCAEVVFICRDNCTLSDQFALINGVWVVVFLANVLIVFTRPWTWPLRLLLGFYSTVGLIFAGHFSQQVLFVRHVLMPRDVAHTSLQLFITFISLFFLILRIRNCQDLLSDLRLLELPSSDAMTLTPNDLSHASSSTPLSTLSP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW04</accession>
    <accession>D2K3U7</accession>
    <name>IRS1_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein IRS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">IRS1</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text>Acts as a transactivator along with IE2, and is required for oriLyt-dependent DNA replication in the transient transfection replication assay using native promoters.</text>
    </comment>
    <comment type="subunit">
        <text>Interacts (via N-terminus) with the viral DNA polymerase accessory subunit UL44. Interacts (via C-terminus) with host EIF2AK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <text evidence="1">Isoform IRS1-263 is predominantly nuclear.</text>
    </comment>
    <comment type="alternative products">
        <event type="alternative initiation"/>
        <isoform>
            <id>Q6SW04-1</id>
            <name>IRS1</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q6SW04-2</id>
            <name>IRS1-263</name>
            <sequence type="described" ref="VSP_043921"/>
        </isoform>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae US22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081587.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BioGRID" id="1678127">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW04"/>
    <dbReference type="GeneID" id="3077574"/>
    <dbReference type="KEGG" id="vg:3077574"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030430">
        <property type="term" value="C:host cell cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030291">
        <property type="term" value="F:protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039580">
        <property type="term" value="P:suppression by virus of host PKR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039502">
        <property type="term" value="P:suppression by virus of host type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0024">Alternative initiation</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-1114">Inhibition of host interferon signaling pathway by virus</keyword>
    <keyword id="KW-1102">Inhibition of host PKR by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000417831" description="Protein IRS1">
        <location>
            <begin position="1"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="607"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="715"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="607"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="734"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="752"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_043921" description="In isoform IRS1-263." evidence="3">
        <location>
            <begin position="1"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="847" mass="91190" checksum="2B699C571D00C980" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW05</accession>
    <accession>D2K3U6</accession>
    <name>UL150_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL150</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL150</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081586.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW05"/>
    <dbReference type="GeneID" id="3077550"/>
    <dbReference type="KEGG" id="vg:3077550"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418304" description="Uncharacterized protein UL150">
        <location>
            <begin position="17"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="432"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="432"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="467"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="639" mass="70082" checksum="116E2448151CEB0A" modified="2004-07-05" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW10</accession>
    <accession>D2K3U1</accession>
    <name>UL133_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL133</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL133</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="PLoS Pathog." volume="7" first="E1002444" last="E1002444">
            <title>A novel human cytomegalovirus locus modulates cell type-specific outcomes of infection.</title>
            <authorList>
                <person name="Umashankar M."/>
                <person name="Petrucelli A."/>
                <person name="Cicchini L."/>
                <person name="Caposio P."/>
                <person name="Kreklywich C.N."/>
                <person name="Rak M."/>
                <person name="Bughio F."/>
                <person name="Goldman D.C."/>
                <person name="Hamlin K.L."/>
                <person name="Nelson J.A."/>
                <person name="Fleming W.H."/>
                <person name="Streblow D.N."/>
                <person name="Goodrum F."/>
            </authorList>
            <dbReference type="PubMed" id="22241980"/>
            <dbReference type="DOI" id="10.1371/journal.ppat.1002444"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>FIX</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host Golgi apparatus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081581.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW10"/>
    <dbReference type="PRIDE" id="Q6SW10"/>
    <dbReference type="DNASU" id="3077577"/>
    <dbReference type="GeneID" id="3077577"/>
    <dbReference type="KEGG" id="vg:3077577"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418328" description="Protein UL133">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="149"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="22241980"/>
        </source>
    </evidence>
    <sequence length="257" mass="27582" checksum="556DE0EE9AC86F7A" modified="2004-07-05" version="1">MGCDVHDPSWQCQWGVPTIIVAWITCAALGIWCLAGSSADVSSGPGIAAVVGCSVFMIFLCAYLIRYREFFKDSVIDLLTCRWVRYCSCSCKCSCKCISGPCSRCCSACYKETMIYDMVQYGHRRRPGHGDDPDRVICEIVESPPVSAPTVFVPPPSEESHQPVIPPQPPTPTSEPKPKKGRAKDKPKGRPKNKPPCEPTVSSQPPSQPTAMPGGPPDASPPAMPQMPPGVAEAVQAAVQAAMAAALQQQQQHQTGT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW29</accession>
    <accession>D2K3S1</accession>
    <name>VIE2_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Viral transcription factor IE2</fullName>
            <shortName>IE2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein UL122</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">UL122</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Stimulates viral early and late gene expression and thus play a crucial role in the regulation of productive infection. In addition, activates quiescent cells to reenter the cell cycle and up-regulates several E2F-responsive genes, which are responsible for pushing the cell into S phase. In S-phase, inhibits cellular DNA synthesis and blocks further cell cycle progression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host SUMO-modified form of TATA-binding protein (TBP)-associated factor 12/TAF12 in a SIM-dependent manner; this interaction increases the transactivation activity of IE2. Interacts with host CHAF1A. Interacts with several components of the host transcriptional machinery including TBP, TF2B and CREB1. Interacts with host DNA replication licensing factor MCM3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Host nucleus</location>
        </subcellularLocation>
        <text evidence="1">Colocalizes with host PML-associated nuclear bodies.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The SUMO-interacting motif (SIM) is required for efficient transactivation function.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Sumoylated. The sumoylation is necessary for efficient replication of the virus and thus for the function of this viral transcription factor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 IE2 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081561.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW29"/>
    <dbReference type="BioGRID" id="1678116">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="IntAct" id="Q6SW29">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW29"/>
    <dbReference type="GeneID" id="3077563"/>
    <dbReference type="KEGG" id="vg:3077563"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010855">
        <property type="entry name" value="Cytomega_IE1/IE2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005028">
        <property type="entry name" value="Herpes_IE2_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07340">
        <property type="entry name" value="Herpes_IE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03361">
        <property type="entry name" value="Herpes_IE2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-1077">G0/G1 host cell cycle checkpoint dysregulation by virus</keyword>
    <keyword id="KW-1078">G1/S host cell cycle checkpoint dysregulation by virus</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1017">Isopeptide bond</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1121">Modulation of host cell cycle by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0832">Ubl conjugation</keyword>
    <feature type="chain" id="PRO_0000417856" description="Viral transcription factor IE2">
        <location>
            <begin position="1"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="99"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Non-covalent SUMO1 binding region (SIM)" evidence="1">
        <location>
            <begin position="200"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="99"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="306"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)" evidence="1">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)" evidence="1">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="580" mass="63097" checksum="215BAFA207681B77" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW31</accession>
    <accession>D2K3R9</accession>
    <name>UL120_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Membrane protein UL120</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL120</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HHV-5 UL120 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081559.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW31"/>
    <dbReference type="DNASU" id="3077520"/>
    <dbReference type="GeneID" id="3077520"/>
    <dbReference type="KEGG" id="vg:3077520"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418242" description="Membrane protein UL120">
        <location>
            <begin position="26"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="26"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="201" mass="23134" checksum="63F0843959092F39" modified="2004-07-05" version="1" precursor="true">MYRAGVTLLVVAVVSFGRWDSVTVATTIRVGWWYEPQVKMAYIYEHNDTNLTIFCNTTAYDSPFLASGMMIVLPHRTQFLTRKVNYSEDMENIKQNYTHQLTHMLTGEPGTYVNGSVTCWGSNGTFGAGTFIVRSMVNKTAGNTNTFIHFVEDSELVENPAYFRRSDHRAFMIVILTQVVFVVFIINASFIWSWTFRRHKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW34</accession>
    <accession>D2K3R6</accession>
    <name>UL116_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL116</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL116</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 US34A protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081556.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW34"/>
    <dbReference type="DNASU" id="3077556"/>
    <dbReference type="GeneID" id="3077556"/>
    <dbReference type="KEGG" id="vg:3077556"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000416713" description="Uncharacterized protein UL116">
        <location>
            <begin position="25"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="1">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="313" mass="34204" checksum="1DAB639A5BD3A7EA" modified="2004-07-05" version="1" precursor="true">MKRRRRWRGWLLFLALCFCLLCEAVETNATTVTSTTAAAATTNTTVATTGTTTTSPNVTSTTSNTVTTPTTVSSVSNLTSSATSILISTSTVSGTRNTRNNNTTTIGTNATSPSSSVSILTTVTPAATSTTSNNGDVTSDYTPTFDLENITTTRAPTRPPAQDLCSHNLSIILYEEESQSSVDIAVDEEEPELEDDDEYDELWFPLYFEAECNLNYTLQYVNHSCDYSVRQSSVSFPPWRDIDSVTFVPRNLSNCSAHGLAVIVAGNQTWYVNPFSLAHLLDAIYNVLGIEDLSANFWRQLAPYRHTLIVPQT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW50</accession>
    <accession>D2K3Q0</accession>
    <name>CVC1_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Capsid vertex component 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">CVC1</name>
        <name type="ordered locus">UL93</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Capsid vertex-specific component that plays a role during viral DNA encapsidation, assuring correct genome cleavage and presumably stabilizing capsids that contain full-length viral genomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts (via C-terminus) with capsid vertex component 2/CVC2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae CVC1 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081540.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW50"/>
    <dbReference type="PRIDE" id="Q6SW50"/>
    <dbReference type="GeneID" id="3077486"/>
    <dbReference type="KEGG" id="vg:3077486"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042025">
        <property type="term" value="C:host cell nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019028">
        <property type="term" value="C:viral capsid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019033">
        <property type="term" value="C:viral tegument"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="Reactome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019076">
        <property type="term" value="P:viral release from host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04017">
        <property type="entry name" value="HSV_CVC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007640">
        <property type="entry name" value="UL17-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04559">
        <property type="entry name" value="Herpes_UL17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0426">Late protein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0231">Viral genome packaging</keyword>
    <keyword id="KW-1188">Viral release from host cell</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000417849" description="Capsid vertex component 1">
        <location>
            <begin position="1"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="176"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="443"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="575"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="594" mass="68501" checksum="F4EE0287D93B955D" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW59</accession>
    <accession>D2K3P1</accession>
    <name>PP65_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>65 kDa phosphoprotein</fullName>
            <shortName>pp65</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>65 kDa matrix phosphoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Phosphoprotein UL83</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Tegument protein UL83</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">UL83</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2010-04" db="PDB data bank">
            <title>Crystal Structure of MHC class I HLA-A2 molecule complexed with short peptide.</title>
            <authorList>
                <person name="Reiser J.-B."/>
                <person name="Le Gorrec M."/>
                <person name="Chouquet A."/>
                <person name="Debeaupuis E."/>
                <person name="Echasserieau K."/>
                <person name="Saulquin X."/>
                <person name="Bonneville M."/>
                <person name="Housset D."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) OF 495-503</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Counteracts the host antiviral immune response when activated and phosphorylated, by preventing host IRF3 from entering the nucleus. Inhibits also the type I interferon production by inactivating the enzymatic activity of DNA sensor CGAS without affecting STING1. Participates in the transactivation of viral major immediate-early genes by the recruitment of host IFI16 to the promoters pf these genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with host NCL/nucleolin. Interacts with host IFI16. Interacts with host CGAS; this interaction inhibits CGAS enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">As part of the incoming virion, pp65 is targeted to the nucleus immediately after infection. The newly synthesized pp65 is observed in the nucleus until some time after 48 hours postinfection. Thereafter, pp65 is probably exported and accumulates in the cytoplasm. Also found in dense bodies.</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">Phosphorylation may play a role in the localization of the protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the herpesviridae pp65 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081531.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PDB" id="3MR9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.93 A"/>
        <property type="chains" value="P=495-503"/>
    </dbReference>
    <dbReference type="PDB" id="3MRB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.40 A"/>
        <property type="chains" value="P=495-503"/>
    </dbReference>
    <dbReference type="PDB" id="3MRC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="P=495-503"/>
    </dbReference>
    <dbReference type="PDB" id="3MRD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="P=495-503"/>
    </dbReference>
    <dbReference type="PDB" id="3VCL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="C=265-275"/>
    </dbReference>
    <dbReference type="PDBsum" id="3MR9"/>
    <dbReference type="PDBsum" id="3MRB"/>
    <dbReference type="PDBsum" id="3MRC"/>
    <dbReference type="PDBsum" id="3MRD"/>
    <dbReference type="PDBsum" id="3VCL"/>
    <dbReference type="SMR" id="Q6SW59"/>
    <dbReference type="BioGRID" id="1678132">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW59"/>
    <dbReference type="GeneID" id="3077579"/>
    <dbReference type="KEGG" id="vg:3077579"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q6SW59"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008649">
        <property type="entry name" value="Herpes_UL82/UL83"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05784">
        <property type="entry name" value="Herpes_UL82_83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-1092">Inhibition of host IRF3 by virus</keyword>
    <keyword id="KW-1113">Inhibition of host RLR pathway by virus</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000418238" description="65 kDa phosphoprotein">
        <location>
            <begin position="1"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="389"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="534"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Bipartite nuclear localization signal" evidence="1">
        <location>
            <begin position="537"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="472"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="269"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P06725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3VCL"/>
        </source>
    </evidence>
    <sequence length="561" mass="62912" checksum="134FD1500DE40B32" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW63</accession>
    <accession>D2K3N7</accession>
    <name>UL79_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL79</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL79</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the expression of late transcripts bridging viral DNA replication and late gene expression during infection. Functions concordantly with UL87 to initiate transcription from over half of all active viral promoters in late infection, without affecting host transcription. Acts on and binds to viral early-late and late kinetic-class promoters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <text evidence="1">Localizes in replication compartments during virus infection.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the herpesviridae UL79 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081527.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW63"/>
    <dbReference type="DNASU" id="3077474"/>
    <dbReference type="GeneID" id="3077474"/>
    <dbReference type="KEGG" id="vg:3077474"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004290">
        <property type="entry name" value="Herpes_UL79"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03049">
        <property type="entry name" value="Herpes_UL79"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417847" description="Protein UL79">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="295" mass="33831" checksum="451318733F44C54A" modified="2004-07-05" version="1">MMARDEENPAVPRVRTGKFSFTCANHLILQISEKMSRGQPLSSLRLEELKIVRLICVLLFHRGLETLLLRETMNNLGVSDHAVLSRKTPQPYWPHLYRELRQAFPGLDFEAAVFDETRAARLSQRLCHPRLSGGLLTRFVQRHTGLPVVFPEDLARNGNILFSLGTLYGHRLFRLAAFFTRHWGAEAYEPLIRIICQKMWYFYLIGTGKMRITPDAFDIQRSRHETGIFTFIMEDYRTFAGTLSRHPHRPHPQQQQHHHPGPPHPPLSHPASSCLSPEAVLAARALHMPTLANDV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2022-02-23" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW66</accession>
    <accession>D2K3N4</accession>
    <name>UL76_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL76</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL76</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="Arch. Virol." volume="154" first="1143" last="1149">
            <title>The conserved UL24 family of human alpha, beta and gamma herpesviruses induces cell cycle arrest and inactivation of the cyclinB/cdc2 complex.</title>
            <authorList>
                <person name="Nascimento R."/>
                <person name="Dias J.D."/>
                <person name="Parkhouse R.M."/>
            </authorList>
            <dbReference type="PubMed" id="19526192"/>
            <dbReference type="DOI" id="10.1007/s00705-009-0420-y"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">May participate in nuclear egress of viral particles. Plays a role in the dispersal of several host nucleolar proteins including NCL/nucleolin and NPM1. Since deletion of host NCL/nucleolin negatively impact on nuclear egress, UL76 supposedly acts on this process through its effect on host nucleoli (By similarity). Induces cell cycle arrest in host cells at the G2/M phase following by apoptosis. The mechanism involves the inhibition of host mitotic complex cyclinB/CDK1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
            <location evidence="1">Host nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text>Expressed late in the infection cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the herpesviridae UL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081524.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="BioGRID" id="1677989">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW66"/>
    <dbReference type="DNASU" id="3077435"/>
    <dbReference type="GeneID" id="3077435"/>
    <dbReference type="KEGG" id="vg:3077435"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002580">
        <property type="entry name" value="Herpes_UL24"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01646">
        <property type="entry name" value="Herpes_UL24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1079">Host G2/M cell cycle arrest by virus</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0426">Late protein</keyword>
    <keyword id="KW-1121">Modulation of host cell cycle by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000416720" description="Protein UL76">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="19526192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="325" mass="35964" checksum="AAFCDBFB0D97ACA0" modified="2004-07-05" version="1">MPSGCGDDADSTGNALRRLPHVRKRIGKRKHLDIYRRLLRVFPSFVALNRLLGGLFPPELQKYRRRLFIEVRLSRRIPDCVLVFLPPDSGSRGIVYCYVIEFKTTYSDADDQSVRWHATHSLQYAEGLRQLKGALVDFDFLRLPRGGGQVWSVVPSLVFFQQKADRPSFYRAFRSGRFDLCTDSVLDYLGRRQDESVAHLLAATRRRLLRAARGKRAALPRARASAVAGGRGGGNARRGLARGRAHGPGAQTVSASGAEGSGSQGTDLLRGSRRARVRGGGAVEPAVRARRRTVAADAATTTVSSAFVVPRDRRGRSFRRPTRSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW77</accession>
    <accession>D2K3M2</accession>
    <name>DPOL_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>DNA polymerase catalytic subunit</fullName>
            <ecNumber>2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL54</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Replicates viral genomic DNA in the late phase of lytic infection, producing long concatemeric DNA. The replication complex is composed of six viral proteins: the DNA polymerase, processivity factor, primase, primase-associated factor, helicase, and ssDNA-binding protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with the ssDNA-binding protein UL57, the DNA polymerase processivity factor UL44, and the alkaline exonuclease UL98. Interacts with the putative helicase-primase complex composed of UL70, UL102 and UL105 proteins; these interactions may coordinate leading and lagging strand DNA synthesis at the replication fork.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <text evidence="1">the protein is present at discrete sites in nuclei, called replication compartments where viral DNA replication occurs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7"/>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081513.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW77"/>
    <dbReference type="PRIDE" id="Q6SW77"/>
    <dbReference type="DNASU" id="3077501"/>
    <dbReference type="GeneID" id="3077501"/>
    <dbReference type="KEGG" id="vg:3077501"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-1194">Viral DNA replication</keyword>
    <feature type="chain" id="PRO_0000417828" description="DNA polymerase catalytic subunit">
        <location>
            <begin position="1"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="644"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1109"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1112"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1141"/>
            <end position="1159"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1242" mass="137158" checksum="48C8C64299ED10EF" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW85</accession>
    <accession>D2K3L4</accession>
    <name>ITP_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inner tegument protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL47</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an essential role in cytoplasmic secondary envelopment during viral egress. Interacts with the capsid via the large tegument protein/LTP and participates in its transport to the host trans-Golgi network (TGN) where secondary envelopment occurs. Modulates tegumentation and capsid accumulation at the viral assembly complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts (via C-terminus) with the large tegument protein/LTP (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion tegument</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host Golgi apparatus</location>
            <location evidence="1">Host trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the herpesviridae inner tegument protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081505.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW85"/>
    <dbReference type="BioGRID" id="1678074">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW85"/>
    <dbReference type="DNASU" id="3077521"/>
    <dbReference type="GeneID" id="3077521"/>
    <dbReference type="KEGG" id="vg:3077521"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_04043">
        <property type="entry name" value="HSV_ITP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007611">
        <property type="entry name" value="Herpes_U30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034738">
        <property type="entry name" value="HSV_ITP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04523">
        <property type="entry name" value="Herpes_U30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-0920">Virion tegument</keyword>
    <feature type="chain" id="PRO_0000418296" description="Inner tegument protein">
        <location>
            <begin position="1"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with large tegument protein" evidence="1">
        <location>
            <begin position="474"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="902"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_04043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="983" mass="110024" checksum="8227D02C0056A636" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW92</accession>
    <accession>D2K3K7</accession>
    <name>UL40_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL40</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the protection against host NK cell cytotoxicity by up-regulating the cell surface expression of HLA-E independent of TAP (HLA-E has an inhibitory effect on the cytotoxic activity of the NK cell). Promotes also cell surface expression of UL18, another viral protein involved in NK cell evasion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 UL40 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081498.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SW92"/>
    <dbReference type="DNASU" id="3077540"/>
    <dbReference type="GeneID" id="3077540"/>
    <dbReference type="KEGG" id="vg:3077540"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019624">
        <property type="entry name" value="Herpes_UL40"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10682">
        <property type="entry name" value="UL40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1131">Modulation of host NK-cell activity by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418285" description="Protein UL40">
        <location>
            <begin position="36"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="HLA-E-binding epitope" evidence="1">
        <location>
            <begin position="15"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="221" mass="24366" checksum="3112380E3B1E2692" modified="2004-07-05" version="1" precursor="true">MNKFSNTRIGFTCAVMAPRTLILTVGLLCMRIRSLLSSPVETTVTTAGVTSAHGPLCPLVFQGWAYAVYHQGDMVLMTLDVYCCRQTSSNTVVAFSHHPADNTLLIEVGNNTRRHVDGISCQDHFRAQHQDCPAQTVHVRGVNESAFGLTHLQSCCLNEHSQLSERVAYHLKLRPATFGLETWAMYTVGILALGSFSSFYSQIARSLGVLPNDHHYALKKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SW98</accession>
    <accession>D2K3K1</accession>
    <name>UL33_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>G-protein coupled receptor homolog UL33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL33</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Trends Pharmacol. Sci." volume="27" first="56" last="63">
            <title>HCMV-encoded G-protein-coupled receptors as constitutively active modulators of cellular signaling networks.</title>
            <authorList>
                <person name="Vischer H.F."/>
                <person name="Leurs R."/>
                <person name="Smit M.J."/>
            </authorList>
            <dbReference type="PubMed" id="16352349"/>
            <dbReference type="DOI" id="10.1016/j.tips.2005.11.006"/>
        </citation>
        <scope>REVIEW ON FUNCTION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2019" name="J. Biol. Chem." volume="294" first="16297" last="16308">
            <title>The human cytomegalovirus-encoded G protein-coupled receptor UL33 exhibits oncomodulatory properties.</title>
            <authorList>
                <person name="van Senten J.R."/>
                <person name="Bebelman M.P."/>
                <person name="Fan T.S."/>
                <person name="Heukers R."/>
                <person name="Bergkamp N.D."/>
                <person name="van Gasselt P."/>
                <person name="Langemeijer E.V."/>
                <person name="Slinger E."/>
                <person name="Lagerweij T."/>
                <person name="Rahbar A."/>
                <person name="Stigter-van Walsum M."/>
                <person name="Maussang D."/>
                <person name="Leurs R."/>
                <person name="Musters R.J.P."/>
                <person name="van Dongen G.A.M.S."/>
                <person name="Soederberg-Naucler C."/>
                <person name="Wuerdinger T."/>
                <person name="Siderius M."/>
                <person name="Smit M.J."/>
            </authorList>
            <dbReference type="PubMed" id="31519750"/>
            <dbReference type="DOI" id="10.1074/jbc.ra119.007796"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2020" name="Viruses" volume="12" first="0" last="0">
            <title>Human Cytomegalovirus-Encoded G Protein-Coupled Receptor UL33 Facilitates Virus Dissemination via the Extracellular and Cell-to-Cell Route.</title>
            <authorList>
                <person name="van Senten J.R."/>
                <person name="Bebelman M.P."/>
                <person name="van Gasselt P."/>
                <person name="Bergkamp N.D."/>
                <person name="van den Bor J."/>
                <person name="Siderius M."/>
                <person name="Smit M.J."/>
            </authorList>
            <dbReference type="PubMed" id="32486172"/>
            <dbReference type="DOI" id="10.3390/v12060594"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>DISRUPTION PHENOTYPE</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2021" name="J. Cell Sci." volume="134" first="0" last="0">
            <title>CMV-encoded GPCR pUL33 activates CREB and facilitates its recruitment to the MIE locus for efficient viral reactivation.</title>
            <authorList>
                <person name="Krishna B.A."/>
                <person name="Wass A.B."/>
                <person name="Dooley A.L."/>
                <person name="O'Connor C.M."/>
            </authorList>
            <dbReference type="PubMed" id="33199520"/>
            <dbReference type="DOI" id="10.1242/jcs.254268"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>DISRUPTION PHENOTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="5 6 7">G-protein-coupled receptor (vGPCR) that constitutively activates multiple oncogenic signaling pathways including STAT3, AP-1, phospholipase C, NF-kappa-B or cAMP-responsive element (CRE) pathways (PubMed:31519750). Plays an important role in viral reactivation from latency through activation of host CREB1, facilitating its recruitment to the viral major immediate early (MIE) genes. In turn, expression of the MIE-driven genes such as UL123 are de-repressed (PubMed:33199520). Facilitates also virus dissemination via the extracellular and cell-to-cell route (PubMed:32486172).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimerizes with US28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Host cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">Present in the virion assembly compartment (VAC) of HCMV-infected cells.</text>
    </comment>
    <comment type="disruption phenotype">
        <text evidence="6 7">UL33-deficient virus displays a growth defect due to deficiency in extracellular transmission (PubMed:32486172). In addition, UL33 disruption attenuates viral reactivation (PubMed:33199520).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081492.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SW98"/>
    <dbReference type="PRIDE" id="Q6SW98"/>
    <dbReference type="DNASU" id="3077468"/>
    <dbReference type="GeneID" id="3077468"/>
    <dbReference type="KEGG" id="vg:3077468"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030430">
        <property type="term" value="C:host cell cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020002">
        <property type="term" value="C:host cell plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039553">
        <property type="term" value="P:suppression by virus of host chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0297">G-protein coupled receptor</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1032">Host cell membrane</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1086">Inhibition of host chemokines by virus</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0675">Receptor</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0807">Transducer</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000416441" description="G-protein coupled receptor homolog UL33">
        <location>
            <begin position="1"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=1" evidence="2">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="2">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=2" evidence="2">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="2">
        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=3" evidence="2">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="2">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=4" evidence="2">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="2">
        <location>
            <begin position="169"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=5" evidence="2">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="2">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=6" evidence="2">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="topological domain" description="Virion surface" evidence="2">
        <location>
            <begin position="266"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=7" evidence="2">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="topological domain" description="Intravirion" evidence="2">
        <location>
            <begin position="314"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="377"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="105"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="31519750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="32486172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="33199520"/>
        </source>
    </evidence>
    <sequence length="412" mass="46080" checksum="6ADD072CF5E5C928" modified="2004-07-05" version="1">MDTIIHNTTNRSTDTPHVNITCNITEPLSAIRTTEAVINTFIIFVGGPLNAIVLITQLLTNRVLGYSTPTIYMTNLYSTNFLTLTVLPFIVLSNQWLLPASVASCKFLSVIYYSSCTVGFATVALIAADRYRVLHKRTYARQSYRSTYIILLLTWFAGLIFSMPAAVYTTVVIHNGTNGQSSNGHATCVLYFIADEVYTVLLSWKVLLTLVWGAAPVIMMTWFYAFFYSTVQRASQKQRSRTLTFVSVLLISFVALQTPYVSIMIFNSYATAAWPMDCEHLTLRRTIGTLSRLVPHLHCLINPILYALLGHDFLQRMRQCFRGQLLDRRAFLRSQQNQRATAETNLAAGNNSQSVATSLDTSSKNCNQHAKRSVSFNFPSGTWKGGQKTASNDTSTKIPHRLSQSHHNLSGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWA0</accession>
    <accession>D2K3J9</accession>
    <name>UL31_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein UL31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL31</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the inhibition of host innate immune system by targeting host CGAS and promoting dissociation of DNA from CGAS, thereby inhibiting the enzymatic activity of CGAS.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host CGAS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the herpesviridae U10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081490.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWA0"/>
    <dbReference type="DNASU" id="3077508"/>
    <dbReference type="GeneID" id="3077508"/>
    <dbReference type="KEGG" id="vg:3077508"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007578">
        <property type="entry name" value="Herpes_U10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04489">
        <property type="entry name" value="DUF570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1048">Host nucleus</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418283" description="Protein UL31">
        <location>
            <begin position="41"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="47"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="595" mass="65790" checksum="324423FBC2158808" modified="2004-07-05" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-02-23" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWB7</accession>
    <accession>D2K3I3</accession>
    <name>UL14_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL14</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHV-5 UL14 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081473.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q6SWB7"/>
    <dbReference type="PRIDE" id="Q6SWB7"/>
    <dbReference type="DNASU" id="3077543"/>
    <dbReference type="GeneID" id="3077543"/>
    <dbReference type="KEGG" id="vg:3077543"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031918">
        <property type="entry name" value="UL141"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038504">
        <property type="entry name" value="UL141-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16758">
        <property type="entry name" value="UL141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000417839" description="Uncharacterized protein UL14">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="327" mass="36699" checksum="BB468D635CCCAF6B" modified="2004-07-05" version="1">MGGGRLPPLWLPLLIAWSEWGNCCLDAPPVVRSPCLQPVRDRNRERNPGSPQLLPYGDRLEVACIFPAHDWPEVSIRVHLCYWPEIVRSLVVDARSGQVLHNDASCYIAGGRWRFEDGGAAQRLSLSFRLITETAGTYTCVLGNETHSLATETTALVADVHDLRHSDRSCDLAFGSRSQTRYLWTPDPSRLRSINCGWEGERHRVVHYIPGTSGLLPSCEEDERELCVPFISQSIADNNCSRRHRVDGARRRYHLRRDYWLTDPKIGLLAAGSVALTSLCHLLCYWCSESYRRLNTEEENEAAEETAAGEASAVAAAAVSEEEQQRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWC5</accession>
    <accession>D2K3H5</accession>
    <name>UL05_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Protein UL5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL5</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in rearrangement of cellular cytoskeleton towards an efficient viral assembly and spreading.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with host IQGAP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Localizes within the assembly compartments within host cytoplasm, but is not incorporated in the virion.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081465.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWC5"/>
    <dbReference type="DNASU" id="3077425"/>
    <dbReference type="GeneID" id="3077425"/>
    <dbReference type="KEGG" id="vg:3077425"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030430">
        <property type="term" value="C:host cell cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016497">
        <property type="entry name" value="Herpes_UL5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006846">
        <property type="entry name" value="UCP006846_UL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418274" description="Protein UL5">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16776"/>
        </source>
    </evidence>
    <sequence length="166" mass="18927" checksum="7966BD2400DB800B" modified="2004-07-05" version="1">MFLGYSDCVDPGLAVYRVSRSRLKLMLSFVWLVGLRFHDCAAFESCCYDITEAESNKAISRDEAAFTSSVSTRTPSLAIAPPPDRSMLLSREEELVPWSRLIITKQFYGGLIFHTTWVTGFVLLGLLTLFASLFRVPQSICRFCIDRLRDIARPLKYRYQRLVATV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWC6</accession>
    <accession>D2K3H4</accession>
    <name>UL04_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Envelope glycoprotein UL4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL4</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">N-glycosylated and possibly O-glycosylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081464.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWC6"/>
    <dbReference type="DNASU" id="3077421"/>
    <dbReference type="GeneID" id="3077421"/>
    <dbReference type="KEGG" id="vg:3077421"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0244">Early protein</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000418240" description="Envelope glycoprotein UL4">
        <location>
            <begin position="19"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="2">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="149" mass="17321" checksum="AB690B6190333AB1" modified="2004-07-05" version="1" precursor="true">MMLRTWISLPMVLLDAYCFCIFVSCSISTTTAPVEWKSPDRQIPMNITCANYSGTVNGNVTFRGLQNKTEDFLHWLLGWGHKSICSFFPKLQGNKEQHYMYGITNLTYNCTYDRLTLLNLTTENSGKYYFKREDVNSTFYYSCYNLTVT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWC7</accession>
    <accession>D2K3H3</accession>
    <name>UL02_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein UL2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL2</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Host membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HHV-5 UL2 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081463.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWC7"/>
    <dbReference type="DNASU" id="3077452"/>
    <dbReference type="GeneID" id="3077452"/>
    <dbReference type="KEGG" id="vg:3077452"/>
    <dbReference type="Reactome" id="R-HSA-9610379">
        <property type="pathway name" value="HCMV Late Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013269">
        <property type="entry name" value="Herpes_UL2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08196">
        <property type="entry name" value="UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000418239" description="Uncharacterized protein UL2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="59" mass="6696" checksum="170D6349722F5F10" modified="2004-07-05" version="1">MAEDSVTILIVEDDDAYPSFGSLPASHAQYGFRLLRSIFLIMLVIWTAVWLKLLRDALL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWC8</accession>
    <accession>D2K3H2</accession>
    <name>UL01_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Glycoprotein UL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">UL1</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Merlin</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Virion membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081462.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWC8"/>
    <dbReference type="DNASU" id="3077477"/>
    <dbReference type="GeneID" id="3077477"/>
    <dbReference type="KEGG" id="vg:3077477"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="SSF48726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0426">Late protein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0261">Viral envelope protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000417833" description="Glycoprotein UL1">
        <location>
            <begin position="28"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="218" mass="24828" checksum="2CE71614BA59ED28" modified="2004-07-05" version="1" precursor="true">MGVQCNSKLLLLAVLITIILSSILVQAIPHKQKTSYQQLLLQSEHVQIPITVAEGDTICFNVSDNPCNFSSYWNHNNCELCGWTPFYSEYAGYSENKSCHPRFTCLHDTKGLKLHNVTTNDSGIYTRNVYYCDIPCNISDDHKHNVEDFDNCNTTINRTHYIITVSSSRYSKRTNSHVATHVGWTATVVIIICVLTYVNVTTTLKHRLRTRNNVNHTM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWD3</accession>
    <accession>D2K3G7</accession>
    <name>IR06_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein IRL6</fullName>
            <shortName>TRL6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RL6</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HHV-5 IRL6 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081457.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWD3"/>
    <dbReference type="DNASU" id="3077544"/>
    <dbReference type="GeneID" id="3077544"/>
    <dbReference type="KEGG" id="vg:3077544"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024349">
        <property type="entry name" value="HHV5_RL5A/RL6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11088">
        <property type="entry name" value="RL11D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418236" description="Uncharacterized protein IRL6">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="111" mass="12256" checksum="FA105E917C4B0980" modified="2009-09-22" version="1">MTLIYVDADNTRNELKATTGSNFTITHRKDPLTTKWKTVFGNNGDQWLCNVTGIGNATVNGNATICVSSCGHNTLDLCNLKSGDSGFFDLSRWFGENMDEYSGDVWHLEVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2022-02-23" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q6SWD5</accession>
    <accession>D2K3G5</accession>
    <name>IR01_HCMVM</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein HKLF1</fullName>
            <shortName>IRL1</shortName>
            <shortName>TRL1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RL1</name>
    </gene>
    <organism>
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HHV-5 HKLF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AAR31561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_081455.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q6SWD5"/>
    <dbReference type="DNASU" id="3077430"/>
    <dbReference type="GeneID" id="3077430"/>
    <dbReference type="KEGG" id="vg:3077430"/>
    <dbReference type="Reactome" id="R-HSA-9609690">
        <property type="pathway name" value="HCMV Early Events"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016394">
        <property type="entry name" value="HHV5_HKLF1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003440">
        <property type="entry name" value="UCP003440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418234" description="Uncharacterized protein HKLF1">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="310" mass="34733" checksum="23A1EECBF4314675" modified="2004-07-05" version="1">MPATDTNSTHTTPLHPEDQHTLPLHHSTTQPHVQTSDKHADKQHRTQMELDAADYAACAQARQHLYGQTQPQLHAYPNANPQESAHFRTENQHQLTNLLHNIGEGAALGYPVPRAEIRRGGGDWADSASDFDADCWCMWGRFGTMGRQPVVTLLLARQRDGLADWNVVRCRGTGFRAHDSEDGVSVWRQHLVFLLGGHGRRVQLERPSAGEAQARGLLPRIRITPISTSPRPKPPQPTTSTASHPHATARPDHTLFPVPSTPSATVHNPRNYAVQLHAETTRTWRWARRGERGAWMPAETFTCPKDKRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-11-13" modified="2020-08-12" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>U3KRG9</accession>
    <name>U3KRG9_HCMVM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein UL30A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">UL30A</name>
        <name evidence="1" type="ORF">HHV5wtgp035a</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Human cytomegalovirus (strain Merlin)</name>
        <name type="common">HHV-5</name>
        <name type="synonym">Human herpesvirus 5</name>
        <dbReference type="NCBI Taxonomy" id="295027"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Duplodnaviria</taxon>
            <taxon>Heunggongvirae</taxon>
            <taxon>Peploviricota</taxon>
            <taxon>Herviviricetes</taxon>
            <taxon>Herpesvirales</taxon>
            <taxon>Herpesviridae</taxon>
            <taxon>Betaherpesvirinae</taxon>
            <taxon>Cytomegalovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2003" name="J. Gen. Virol." volume="84" first="657" last="663">
            <title>Homology between the human cytomegalovirus RL11 gene family and human adenovirus E3 genes.</title>
            <authorList>
                <person name="Davison A.J."/>
                <person name="Akter P."/>
                <person name="Cunningham C."/>
                <person name="Dolan A."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Wilkinson G.W."/>
            </authorList>
            <dbReference type="PubMed" id="12604818"/>
            <dbReference type="DOI" id="10.1099/vir.0.18856-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Merlin</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2003" name="J. Gen. Virol." volume="84" first="1117" last="1122">
            <title>Two novel spliced genes in human cytomegalovirus.</title>
            <authorList>
                <person name="Akter P."/>
                <person name="Cunningham C."/>
                <person name="McSharry B.P."/>
                <person name="Dolan A."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="12692276"/>
            <dbReference type="DOI" id="10.1099/vir.0.18952-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Merlin</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="3">
        <citation type="journal article" date="2003" name="J. Gen. Virol." volume="84" first="2511" last="2516">
            <title>The most abundantly transcribed human cytomegalovirus gene (beta 2.7) is non-essential for growth in vitro.</title>
            <authorList>
                <person name="McSharry B.P."/>
                <person name="Tomasec P."/>
                <person name="Neale M.L."/>
                <person name="Wilkinson G.W."/>
            </authorList>
            <dbReference type="PubMed" id="12917473"/>
            <dbReference type="DOI" id="10.1099/vir.0.19298-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Merlin</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="4">
        <citation type="journal article" date="2004" name="J. Gen. Virol." volume="85" first="1301" last="1312">
            <title>Genetic content of wild-type human cytomegalovirus.</title>
            <authorList>
                <person name="Dolan A."/>
                <person name="Cunningham C."/>
                <person name="Hector R.D."/>
                <person name="Hassan-Walker A.F."/>
                <person name="Lee L."/>
                <person name="Addison C."/>
                <person name="Dargan D.J."/>
                <person name="McGeoch D.J."/>
                <person name="Gatherer D."/>
                <person name="Emery V.C."/>
                <person name="Griffiths P.D."/>
                <person name="Sinzger C."/>
                <person name="McSharry B.P."/>
                <person name="Wilkinson G.W."/>
                <person name="Davison A.J."/>
            </authorList>
            <dbReference type="PubMed" id="15105547"/>
            <dbReference type="DOI" id="10.1099/vir.0.79888-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Merlin</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AY446894">
        <property type="protein sequence ID" value="AGL13510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_007947989.1">
        <property type="nucleotide sequence ID" value="NC_006273.2"/>
    </dbReference>
    <dbReference type="SMR" id="U3KRG9"/>
    <dbReference type="GeneID" id="15463999"/>
    <dbReference type="KEGG" id="vg:15463999"/>
    <dbReference type="Proteomes" id="UP000000938">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGL13510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000938"/>
        </source>
    </evidence>
    <sequence length="79" mass="9470" checksum="8709B1C3F4D14E49" modified="2013-11-13" version="1">MACRDARWVRFIRLRTLWSVRPRAHVADLEDFAAFYRTLSDSEQQEFEQEAELASRSQRVRDLREARRQLKMDLMCHGG</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>