<?xml version="1.0" encoding="UTF-8"?>
<uniprot xmlns="http://uniprot.org/uniprot"
 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
 xsi:schemaLocation="http://uniprot.org/uniprot http://www.uniprot.org/docs/uniprot.xsd">
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>P0C748</accession>
    <name>VP5_DXV96</name>
    <protein>
        <recommendedName>
            <fullName>Protein VP5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">VP5</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila x virus (isolate Chung/1996)</name>
        <name type="common">DXV</name>
        <dbReference type="NCBI Taxonomy" id="654931"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Birnaviridae</taxon>
            <taxon>Entomobirnavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Drosophila melanogaster</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="7227"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1996" name="Virology" volume="225" first="359" last="368">
            <title>Sequence analysis of the bicistronic Drosophila X virus genome segment A and its encoded polypeptides.</title>
            <authorList>
                <person name="Chung H.K."/>
                <person name="Kordyban S."/>
                <person name="Cameron L."/>
                <person name="Dobos P."/>
            </authorList>
            <dbReference type="PubMed" id="8918922"/>
            <dbReference type="DOI" id="10.1006/viro.1996.0610"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Host membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="U60650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000515">
        <property type="component" value="Genome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000378415" description="Protein VP5">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="166"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="237" mass="27060" checksum="8D80A8A25BCFDB32" modified="2009-07-07" version="1">MLSIIRRKTRIVDITKQGNGNVPPACHLCSRRLTKEWSFGEGTICSFHRIRCRVQCGRSFWHQPETPIDGLSWDSTTWELTRISSSKDPDISGQKDKRYGRRKEKNPKTDPPALDSRVREHEPIHEHEPIPGRVGPADTKQRCKANLRGDSGFVSIGRSNHPKLSREDCHNTRVPPGTQGVRGGNVQLDKPRERPVSYQHGSKKRSEHRNPVSRSHRRKKAKTRTKTSKLGKSRDIC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q91CD5</accession>
    <name>RDRP_DXV96</name>
    <protein>
        <recommendedName>
            <fullName>RNA-directed RNA polymerase</fullName>
            <shortName>RDRP</shortName>
            <ecNumber>2.7.7.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Protein VP1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">VP1</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila x virus (isolate Chung/1996)</name>
        <name type="common">DXV</name>
        <dbReference type="NCBI Taxonomy" id="654931"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Birnaviridae</taxon>
            <taxon>Entomobirnavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Drosophila melanogaster</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="7227"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2002" name="Virology" volume="296" first="241" last="250">
            <title>Birnavirus VP1 proteins form a distinct subgroup of RNA-dependent RNA polymerases lacking a GDD motif.</title>
            <authorList>
                <person name="Shwed P.S."/>
                <person name="Dobos P."/>
                <person name="Cameron L.A."/>
                <person name="Vakharia V.N."/>
                <person name="Duncan R."/>
            </authorList>
            <dbReference type="PubMed" id="12069523"/>
            <dbReference type="DOI" id="10.1006/viro.2001.1334"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">RNA-dependent RNA polymerase which is found both free and covalently attached to the genomic RNA. May also contain guanylyl and methyl transferase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with VP3 in the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
        <text evidence="1">Minor amounts are incorporated in the virion.</text>
    </comment>
    <comment type="PTM">
        <text>May exist in multiple phosphorylated forms.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48"/>
    <dbReference type="EMBL" id="AF196645">
        <property type="protein sequence ID" value="AAL13168.2"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_690806.1">
        <property type="nucleotide sequence ID" value="NC_004169.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q91CD5"/>
    <dbReference type="GeneID" id="993339"/>
    <dbReference type="KEGG" id="vg:993339"/>
    <dbReference type="Proteomes" id="UP000000515">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018144">
        <property type="term" value="P:RNA-protein covalent cross-linking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019079">
        <property type="term" value="P:viral genome replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007100">
        <property type="entry name" value="RNA-dir_pol_birnavirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007098">
        <property type="entry name" value="RNA-dir_pol_mononegavirus"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04197">
        <property type="entry name" value="Birna_RdRp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50524">
        <property type="entry name" value="RDRP_DSRNA_BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0191">Covalent protein-RNA linkage</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0693">Viral RNA replication</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000378412" description="RNA-directed RNA polymerase">
        <location>
            <begin position="1"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="domain" description="RdRp catalytic" evidence="3">
        <location>
            <begin position="420"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="950"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="950"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="256"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="997" mass="112779" checksum="2CE74CA091D9AC29" modified="2002-03-01" version="2">MSDIFNQQGLKSKFSNIVKNEGQGDSDIREVFNELARPQAEVGRDIEYSELSQALNDNSVKVFRPQPYDELVNIPFIEAASPRMMALYGELLDSKDVSLPVGSGLHIPTYKPGHEVTPPLLTLPNSLSYEYMHYIANSANDTWDKQIYETLKELLVAQATNRFSTGSLLGQVKRVAAGQDVAYGRKGHHKNKSFKEMGITPYRVMEWLDEYMPISDDEPEITLSNTLDWLVYTPEEAELMGVPDSLPDITQSSAAGLPWLGKKKGEVAVSALITANMLIKDVSQLLKENLFTGSNNPLDPKKEGVAEVTTKNPRRAADQFSRQVLDRIIKEYSYTMMGLLFPKGERYAIADHLTKTRNIWSASYVTHLIGSTISDQPAKRMLNVLTSTSRTPSLAKFSPTQGGMEALINIILNATDIVELVYADNAYIYYPNEDIWYSIDLTKGEANCTRDVAMTTAMYLLTRGWTSKQGTPIYNYTWAYLALYGIPYMTVDSISVLKNFQIKNPGQGSGNPWTFLNNHVLTTILMNKWSEIGKPQPSPDVIEKLASMTGIDFKVELVVTNFREKLIAASRHSIPTSNRVEPRTIVEMDMLGWDVTHTEFGFTPVLSKERLFKSIACPQPPSSTFQTSVAKQVHKYIQNVALLYVGAWAYPCIAQTIEGYALNHWNTINIMIRNKEYDLDKAIGKAVEASPFSEVISLLSLDRPMHEQNYAQVLYQQKTIEKKEAKPKVSNPLYKRDKETYHEYTTRMARMRINDDMVGPQWEPIINLVTKLYPREATGENQKSRESMTRVKIRGLLENMERQLESNGRSMDVWYNAYLTGKKPSGVDKKLATLLVLLAPKRTKKLPSGVYQKLLGYPPIDKSPVPSLTSDEAYLYDTNSLEYNRIAYVSKLNEDDITVYANKYMVYSSTLLSNLLPDKVDWPELRSMNVEGASDPYQVKGYKKKELKPRFGEEILDDEPTGKKSSSEKRRLQRKGQKAKLQRQAATGTTFVRKPLN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q96724</accession>
    <name>POLS_DXV96</name>
    <protein>
        <recommendedName>
            <fullName>Structural polyprotein</fullName>
            <shortName>PP</shortName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Precursor of VP2</fullName>
                <shortName>pre-VP2</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Capsid protein VP2</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Structural peptide 1</fullName>
                <shortName>p1</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Structural peptide 2</fullName>
                <shortName>p2</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Structural peptide 3</fullName>
                <shortName>p3</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protease VP4</fullName>
                <ecNumber>3.4.21.-</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>Non-structural protein VP4</fullName>
                <shortName>NS</shortName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Capsid protein VP3</fullName>
            </recommendedName>
        </component>
    </protein>
    <organism>
        <name type="scientific">Drosophila x virus (isolate Chung/1996)</name>
        <name type="common">DXV</name>
        <dbReference type="NCBI Taxonomy" id="654931"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Birnaviridae</taxon>
            <taxon>Entomobirnavirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Drosophila melanogaster</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="7227"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1996" name="Virology" volume="225" first="359" last="368">
            <title>Sequence analysis of the bicistronic Drosophila X virus genome segment A and its encoded polypeptides.</title>
            <authorList>
                <person name="Chung H.K."/>
                <person name="Kordyban S."/>
                <person name="Cameron L."/>
                <person name="Dobos P."/>
            </authorList>
            <dbReference type="PubMed" id="8918922"/>
            <dbReference type="DOI" id="10.1006/viro.1996.0610"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Capsid protein VP2 self assembles to form an icosahedral capsid with a T=13 symmetry, about 70 nm in diameter, and consisting of 260 VP2 trimers. The capsid encapsulates the genomic dsRNA. VP2 is also involved in attachment and entry into the host cell.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The precursor of VP2 plays an important role in capsid assembly. First, pre-VP2 and VP2 oligomers assemble to form a procapsid. Then, the pre-VP2 intermediates may be processed into VP2 proteins by proteolytic cleavage mediated by VP4 to obtain the mature virion. The final capsid is composed of pentamers and hexamers but VP2 has a natural tendency to assemble into all-pentameric structures. Therefore pre-VP2 may be required to allow formation of the hexameric structures.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Protease VP4 is a serine protease that cleaves the polyprotein into its final products. Pre-VP2 is first partially cleaved, and may be completely processed by VP4 upon capsid maturation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Capsid protein VP3 plays a key role in virion assembly by providing a scaffold for the capsid made of VP2. May self-assemble to form a T=4-like icosahedral inner-capsid composed of at least 180 trimers. Plays a role in genomic RNA packaging by recruiting VP1 into the capsid and interacting with the dsRNA genome segments to form a ribonucleoprotein complex. Additionally, the interaction of the VP3 C-terminal tail with VP1 removes the inherent structural blockade of the polymerase active site. Thus, VP3 can also function as a transcriptional activator.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Structural peptide 1 is a small peptide derived from pre-VP2 C-terminus. It destabilizes and perforates cell membranes, suggesting a role during entry.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Structural peptide 2 is a small peptide derived from pre-VP2 C-terminus. It is not essential for the virus viability, but viral growth is affected when missing.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Structural peptide 3 is a small peptide derived from pre-VP2 C-terminus. It is not essential for the virus viability, but viral growth is affected when missing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Capsid protein VP2 is a homotrimer. A central divalent metal stabilizes the VP2 trimer (By similarity). Capsid protein VP3 is a homodimer. Capsid protein VP3 interacts (via C-terminus) with VP1 in the cytoplasm. Capsid VP3 interacts with VP2.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Capsid protein VP2</molecule>
        <subcellularLocation>
            <location evidence="4">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Capsid protein VP3</molecule>
        <subcellularLocation>
            <location evidence="4">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Structural peptide 1</molecule>
        <subcellularLocation>
            <location evidence="4">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Structural peptide 2</molecule>
        <subcellularLocation>
            <location evidence="4">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Structural peptide 3</molecule>
        <subcellularLocation>
            <location evidence="4">Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Specific enzymatic cleavages yield mature proteins. The capsid assembly seems to be regulated by polyprotein processing. The protease VP4 cleaves itself off the polyprotein, thus releasing pre-VP2 and VP3 within the infected cell. During capsid assembly, the C-terminus of pre-VP2 is further processed by VP4, giving rise to VP2, the external capsid protein and three small peptides that all stay closely associated with the capsid.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-"/>
    <dbReference type="EMBL" id="U60650">
        <property type="protein sequence ID" value="AAB16798.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_690836.1">
        <property type="nucleotide sequence ID" value="NC_004177.1"/>
    </dbReference>
    <dbReference type="PDB" id="6SHW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=724-1032"/>
    </dbReference>
    <dbReference type="PDB" id="6SI6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.98 A"/>
        <property type="chains" value="A/B=725-1032"/>
    </dbReference>
    <dbReference type="PDBsum" id="6SHW"/>
    <dbReference type="PDBsum" id="6SI6"/>
    <dbReference type="SMR" id="Q96724"/>
    <dbReference type="MEROPS" id="S50.003"/>
    <dbReference type="PRIDE" id="Q96724"/>
    <dbReference type="GeneID" id="993338"/>
    <dbReference type="KEGG" id="vg:993338"/>
    <dbReference type="Proteomes" id="UP000000515">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030430">
        <property type="term" value="C:host cell cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039621">
        <property type="term" value="C:T=13 icosahedral viral capsid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002662">
        <property type="entry name" value="Birna_VP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002663">
        <property type="entry name" value="Birna_VP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025775">
        <property type="entry name" value="Birna_VP4_Prtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029053">
        <property type="entry name" value="Viral_coat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01766">
        <property type="entry name" value="Birna_VP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01767">
        <property type="entry name" value="Birna_VP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01768">
        <property type="entry name" value="Birna_VP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51548">
        <property type="entry name" value="BIRNAVIRUS_VP4_PRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <keyword id="KW-1146">T=13 icosahedral capsid protein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000378376" description="Structural polyprotein">
        <location>
            <begin position="1"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000378377" description="Precursor of VP2">
        <location>
            <begin position="1"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000378378" description="Capsid protein VP2">
        <location>
            <begin position="1"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="peptide" id="PRO_0000378379" description="Structural peptide 1" evidence="1">
        <location>
            <begin position="431"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="peptide" id="PRO_0000378380" description="Structural peptide 2" evidence="1">
        <location>
            <begin position="475"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="peptide" id="PRO_0000378381" description="Structural peptide 3" evidence="1">
        <location>
            <begin position="484"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000378382" description="Protease VP4">
        <location>
            <begin position="501"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000378383" description="Capsid protein VP3">
        <location>
            <begin position="724"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S50" evidence="2">
        <location>
            <begin position="501"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="986"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with VP1 protein" evidence="1">
        <location>
            <begin position="1021"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="627"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="670"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between trimeric partners</note>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by protease VP4" evidence="1">
        <location>
            <begin position="431"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by protease VP4" evidence="1">
        <location>
            <begin position="474"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by protease VP4" evidence="1">
        <location>
            <begin position="483"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by protease VP4" evidence="1">
        <location>
            <begin position="500"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by protease VP4" evidence="1">
        <location>
            <begin position="723"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="769"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="784"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="799"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="6SI6"/>
        </source>
    </evidence>
    <sequence length="1032" mass="114028" checksum="44F5CB82FE191CFB" modified="1997-02-01" version="1">MNTTNEYLKTLLNPAQFISDIPDDIMIRHVNSAQTITYNLKSGASGTGLIVVYPNTPSSISGFHYIWDSATSNWVFDQYIYTAQELKDSYDYGRLISGSLSIKSSTLPAGVYALNGTFNAVWFQGTLSEVSDYSYDRILSITSNPLDKVGNVLVGDGIEVLSLPQGFNNPYVRLGDKSPSTLSSPTHITNTSQNLATGGAYMIPVTTVPGQGFHNKEFSINVDSVGPVDILWSGQMTMQDEWTVTANYQPLNISGTLIANSQRTLTWSNTGVSNGSHYMNMNNLNVSLFHENPPPEPVAAIKININYGNNTNGDSSFSVDSSFTINVIGGATIGVNSPTVGVGYQGVAEGTAITISGINNYELVPNPDLQKNLPMTYGTCDPHDLTYIKYILSNREQLGLRSVMTLADYNRMKMYMHVLTNYHVDEREASSFDFWQLLKQIKNVAVPLAATLAPQFAPIIGAADGLANAILGDSASGRPVGNSASGMPISMSRRLRNAYSADSPLGEEHWLPNENENFNKFDIIYDVSHSSMALFPVIMMEHDKVIPSDPEELYIAVSLTESLRKQIPNLNDMPYYEMGGHRVYNSVSSNVRSGNFLRSDYILLPCYQLLEGRLASSTSPNKVTGTSHQLAIYAADDLLKSGVLGKAPFAAFTGSVVGSSVGEVFGINLKLQLTDSLGIPLLGNSPGLVQVKTLTSLDKKIKDMGDVKRRTPKQTLPHWTAGSASMNPFMNTNPFLEELDQPIPSNAAKPISEETRDLFLSDGQTIPSSQEKIATIHEYLLEHKELEEAMFSLISQGRGRSLINMVVKSALNIETQSREVTGERRQRLERKLRNLENQGIYVDESKIMSRGRISKEDTELAMRIARKNQKDAKLRRIYSNNASIQESYTVDDFVSYWMEQESLPTGIQIAMWLKGDDWSQPIPPRVQRRHYDSYIMMLGPSPTQEQADAVKDLVDDIYDRNQGKGPSQEQARELSHAVRRLISHSLVNQPATAPRVPPRRIVSAQTAQTDPPGRRAALDRLRRVRGEDNDIV</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>