<?xml version="1.0" encoding="UTF-8"?>
<uniprot xmlns="http://uniprot.org/uniprot"
 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
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<entry dataset="Swiss-Prot" created="1992-08-01" modified="2022-08-03" version="141" xmlns="http://uniprot.org/uniprot">
    <accession>P27410</accession>
    <name>POLG_RHDVF</name>
    <protein>
        <recommendedName>
            <fullName>Genome polyprotein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>p254</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Protein p16</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein p23</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>NTPase</fullName>
                <ecNumber>3.6.1.15</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>2C-like protein</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>P2C</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>p37</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Precursor p41</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein p29</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein p23/2</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Protein p18</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Viral genome-linked protein</fullName>
            </recommendedName>
            <alternativeName>
                <fullName>VPg</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>p13</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>3C-like protease</fullName>
                <shortName>3CLpro</shortName>
                <ecNumber>3.4.22.66</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>Calicivirin</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>Thiol protease P3C</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>p15</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>RNA-directed RNA polymerase</fullName>
                <ecNumber>2.7.7.48</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>3Dpol</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>p58</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName>Capsid protein VP60</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="ORF">ORF1</name>
    </gene>
    <organism>
        <name type="scientific">Rabbit hemorrhagic disease virus (strain Rabbit/Germany/FRG/1989)</name>
        <name type="common">Ra/LV/RHDV/GH/1989/GE</name>
        <name type="synonym">RHDV-FRG</name>
        <dbReference type="NCBI Taxonomy" id="314536"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Pisuviricota</taxon>
            <taxon>Pisoniviricetes</taxon>
            <taxon>Picornavirales</taxon>
            <taxon>Caliciviridae</taxon>
            <taxon>Lagovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Oryctolagus cuniculus</name>
        <name type="common">Rabbit</name>
        <dbReference type="NCBI Taxonomy" id="9986"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="184" first="664" last="676">
            <title>Rabbit hemorrhagic disease virus -- molecular cloning and nucleotide sequencing of a calicivirus genome.</title>
            <authorList>
                <person name="Meyers G."/>
                <person name="Wirblich C."/>
                <person name="Thiel H.-J."/>
            </authorList>
            <dbReference type="PubMed" id="1840711"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90436-F"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1991" name="Virology" volume="184" first="677" last="686">
            <title>Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles.</title>
            <authorList>
                <person name="Meyers G."/>
                <person name="Wirblich C."/>
                <person name="Thiel H.-J."/>
            </authorList>
            <dbReference type="PubMed" id="1887589"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90437-G"/>
        </citation>
        <scope>FUNCTION OF VPG</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1994" name="J. Virol." volume="68" first="6487" last="6495">
            <title>Identification and characterization of a 3C-like protease from rabbit hemorrhagic disease virus, a calicivirus.</title>
            <authorList>
                <person name="Boniotti B."/>
                <person name="Wirblich C."/>
                <person name="Sibilia M."/>
                <person name="Meyers G."/>
                <person name="Thiel H.J."/>
                <person name="Rossi C."/>
            </authorList>
            <dbReference type="PubMed" id="8083986"/>
        </citation>
        <scope>MUTAGENESIS OF HIS-1135; ASP-1152; ASP-1175; CYS-1212 AND HIS-1227</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1995" name="J. Virol." volume="69" first="7159" last="7168">
            <title>3C-like protease of rabbit hemorrhagic disease virus: identification of cleavage sites in the ORF1 polyprotein and analysis of cleavage specificity.</title>
            <authorList>
                <person name="Wirblich C."/>
                <person name="Sibilia M."/>
                <person name="Boniotti M.B."/>
                <person name="Rossi C."/>
                <person name="Thiel H.-J."/>
                <person name="Meyers G."/>
            </authorList>
            <dbReference type="PubMed" id="7474137"/>
        </citation>
        <scope>PROTEOLYTIC PROCESSING OF POLYPROTEIN</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1995" name="J. Virol." volume="69" first="5812" last="5815">
            <title>Two independent pathways of expression lead to self-assembly of the rabbit hemorrhagic disease virus capsid protein.</title>
            <authorList>
                <person name="Sibilia M."/>
                <person name="Boniotti M.B."/>
                <person name="Angoscini P."/>
                <person name="Capucci L."/>
                <person name="Rossi C."/>
            </authorList>
            <dbReference type="PubMed" id="7637026"/>
        </citation>
        <scope>SUBGENOMIC ORIGIN OF VP60</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="1998" name="J. Virol." volume="72" first="4492" last="4497">
            <title>Detection of viral proteins after infection of cultured hepatocytes with rabbit hemorrhagic disease virus.</title>
            <authorList>
                <person name="Konig M."/>
                <person name="Thiel H.J."/>
                <person name="Meyers G."/>
            </authorList>
            <dbReference type="PubMed" id="9557747"/>
        </citation>
        <scope>PROTEOLYTIC PROCESSING OF POLYPROTEIN</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2000" name="Virology" volume="276" first="349" last="363">
            <title>Rabbit hemorrhagic disease virus: genome organization and polyprotein processing of a calicivirus studied after transient expression of cDNA constructs.</title>
            <authorList>
                <person name="Meyers G."/>
                <person name="Wirblich C."/>
                <person name="Thiel H.J."/>
                <person name="Thumfart J.O."/>
            </authorList>
            <dbReference type="PubMed" id="11040126"/>
            <dbReference type="DOI" id="10.1006/viro.2000.0545"/>
        </citation>
        <scope>PROTEOLYTIC PROCESSING OF POLYPROTEIN</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2000" name="J. Gen. Virol." volume="81" first="481" last="488">
            <title>Identification of a new cleavage site of the 3C-like protease of rabbit haemorrhagic disease virus.</title>
            <authorList>
                <person name="Joubert P."/>
                <person name="Pautigny C."/>
                <person name="Madelaine M.-F."/>
                <person name="Rasschaert D."/>
            </authorList>
            <dbReference type="PubMed" id="10644847"/>
        </citation>
        <scope>PROTEOLYTIC PROCESSING OF POLYPROTEIN</scope>
    </reference>
    <reference key="9">
        <citation type="journal article" date="2001" name="Arch. Virol." volume="146" first="59" last="69">
            <title>Characterisation of the RNA-dependent RNA polymerase from Rabbit hemorrhagic disease virus produced in Escherichia coli.</title>
            <authorList>
                <person name="Lopez Vazquez A.L."/>
                <person name="Martin Alonso J.M."/>
                <person name="Parra F."/>
            </authorList>
            <dbReference type="PubMed" id="11266218"/>
            <dbReference type="DOI" id="10.1007/s007050170191"/>
        </citation>
        <scope>CHARACTERIZATION OF RNA-DIRECTED RNA POLYMERASE</scope>
    </reference>
    <reference key="10">
        <citation type="journal article" date="2002" name="Virology" volume="304" first="352" last="363">
            <title>Rabbit hemorrhagic disease virus: identification of a cleavage site in the viral polyprotein that is not processed by the known calicivirus protease.</title>
            <authorList>
                <person name="Thumfart J.O."/>
                <person name="Meyers G."/>
            </authorList>
            <dbReference type="PubMed" id="12504575"/>
            <dbReference type="DOI" id="10.1006/viro.2002.1660"/>
        </citation>
        <scope>PROTEOLYTIC PROCESSING OF POLYPROTEIN</scope>
    </reference>
    <reference key="11">
        <citation type="journal article" date="2004" name="Virology" volume="322" first="118" last="134">
            <title>The coat protein of Rabbit hemorrhagic disease virus contains a molecular switch at the N-terminal region facing the inner surface of the capsid.</title>
            <authorList>
                <person name="Barcena J."/>
                <person name="Verdaguer N."/>
                <person name="Roca R."/>
                <person name="Morales M."/>
                <person name="Angulo I."/>
                <person name="Risco C."/>
                <person name="Carrascosa J.L."/>
                <person name="Torres J.M."/>
                <person name="Caston J.R."/>
            </authorList>
            <dbReference type="PubMed" id="15063122"/>
            <dbReference type="DOI" id="10.1016/j.virol.2004.01.021"/>
        </citation>
        <scope>FUNCTION OF CAPSID PROTEIN VP60</scope>
    </reference>
    <reference key="12">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="1381" last="1387">
            <title>Crystal structures of active and inactive conformations of a caliciviral RNA-dependent RNA polymerase.</title>
            <authorList>
                <person name="Ng K.K.S."/>
                <person name="Cherney M.M."/>
                <person name="Vazquez A.L."/>
                <person name="Machin A."/>
                <person name="Martin-Alonso J.M."/>
                <person name="Parra F."/>
                <person name="James M.N.G."/>
            </authorList>
            <dbReference type="PubMed" id="11677245"/>
            <dbReference type="DOI" id="10.1074/jbc.M109261200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1252-1767</scope>
    </reference>
    <comment type="function">
        <text evidence="1">NTPase presumably plays a role in replication.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">3C-like protease processes the polyprotein: 3CLpro-RdRp (p72) is first released by autocleavage, then all other proteins are cleaved.</text>
    </comment>
    <comment type="function">
        <text evidence="2">RNA-directed RNA polymerase replicates genomic and antigenomic RNA by recognizing replications specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Capsid protein VP60 self assembles to form an icosahedral capsid with a T=3 symmetry, about 35 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate VP2 proteins and genomic or subgenomic RNA. Attaches virion to target cells by binding histo-blood group antigens, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="3.6.1.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.</text>
            <dbReference type="EC" id="3.4.22.66"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Binds to histo-blood group antigens at surface of target cells.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Capsid protein VP60</molecule>
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="alternative products">
        <event type="alternative promoter"/>
        <isoform>
            <id>P27410-1</id>
            <name>Genome polyprotein</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>P27410-2</id>
            <name>Subgenomic capsid protein VP60</name>
            <name>VP1</name>
            <sequence type="described" ref="VSP_034381"/>
        </isoform>
    </comment>
    <comment type="PTM">
        <text evidence="6 7 8 9 11">Specific enzymatic cleavages by its own cysteine protease yield mature proteins. The protease cleaves itself from the nascent polyprotein autocatalytically. Precursor p41 can be cleaved by viral 3CLpro into protein p19 and VPg, or cleaved by host protease into protein p23/2 and protein p18.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Two different RNAs lead the expression of the capsid protein. One arises from the cleavage of the polyprotein translated from the genomic RNA and the other from the translation of a subgenomic RNA derived from the (-)RNA template. Capsid protein expressed from the subgenomic mRNA is produced in much larger amounts than the cleaved one.</text>
    </comment>
    <comment type="miscellaneous">
        <molecule>Isoform Genome polyprotein</molecule>
        <text>Produced from the genomic RNA.</text>
    </comment>
    <comment type="miscellaneous">
        <molecule>Isoform Subgenomic capsid protein VP60</molecule>
        <text evidence="12">Produced from the subgenomic RNA.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.15"/>
    <dbReference type="EC" id="3.4.22.66"/>
    <dbReference type="EC" id="2.7.7.48"/>
    <dbReference type="EMBL" id="M67473">
        <property type="protein sequence ID" value="AAA47285.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="A41039">
        <property type="entry name" value="RRWWRH"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_062875.1">
        <property type="nucleotide sequence ID" value="NC_001543.1"/>
    </dbReference>
    <dbReference type="PDB" id="1KHV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=1252-1767"/>
    </dbReference>
    <dbReference type="PDB" id="1KHW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B=1252-1767"/>
    </dbReference>
    <dbReference type="PDB" id="6M24">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.29 A"/>
        <property type="chains" value="C=2271-2279"/>
    </dbReference>
    <dbReference type="PDB" id="6M2J">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="C=2308-2316"/>
    </dbReference>
    <dbReference type="PDB" id="6M2K">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.59 A"/>
        <property type="chains" value="C=2106-2114"/>
    </dbReference>
    <dbReference type="PDBsum" id="1KHV"/>
    <dbReference type="PDBsum" id="1KHW"/>
    <dbReference type="PDBsum" id="6M24"/>
    <dbReference type="PDBsum" id="6M2J"/>
    <dbReference type="PDBsum" id="6M2K"/>
    <dbReference type="SMR" id="P27410"/>
    <dbReference type="MEROPS" id="C24.001"/>
    <dbReference type="PRIDE" id="P27410"/>
    <dbReference type="GeneID" id="1491968"/>
    <dbReference type="KEGG" id="vg:1491968"/>
    <dbReference type="BRENDA" id="3.4.22.B15">
        <property type="organism ID" value="5265"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="P27410"/>
    <dbReference type="Proteomes" id="UP000000413">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030430">
        <property type="term" value="C:host cell cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019028">
        <property type="term" value="C:viral capsid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018144">
        <property type="term" value="P:RNA-protein covalent cross-linking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039694">
        <property type="term" value="P:viral RNA genome replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00205">
        <property type="entry name" value="rhv_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004005">
        <property type="entry name" value="Calicivirus_coat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004004">
        <property type="entry name" value="Helic/Pol/Pept_Calicivir-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000605">
        <property type="entry name" value="Helicase_SF3_ssDNA/RNA_vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014759">
        <property type="entry name" value="Helicase_SF3_ssRNA_vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000317">
        <property type="entry name" value="Peptidase_C24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033703">
        <property type="entry name" value="Rhv-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001205">
        <property type="entry name" value="RNA-dir_pol_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007094">
        <property type="entry name" value="RNA-dir_pol_PSvirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029053">
        <property type="entry name" value="Viral_coat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00915">
        <property type="entry name" value="Calici_coat"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF03510">
        <property type="entry name" value="Peptidase_C24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00680">
        <property type="entry name" value="RdRP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00910">
        <property type="entry name" value="RNA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00916">
        <property type="entry name" value="2CENDOPTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00918">
        <property type="entry name" value="CALICVIRUSNS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51894">
        <property type="entry name" value="CV_3CL_PRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50507">
        <property type="entry name" value="RDRP_SSRNA_POS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51218">
        <property type="entry name" value="SF3_HELICASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0877">Alternative promoter usage</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-0191">Covalent protein-RNA linkage</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword id="KW-0788">Thiol protease</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0693">Viral RNA replication</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000341645" description="Genome polyprotein">
        <location>
            <begin position="1"/>
            <end position="2344"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000036959" description="Protein p16">
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            <begin position="1"/>
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    </feature>
    <feature type="chain" id="PRO_0000036960" description="Protein p23">
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            <begin position="144"/>
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    </feature>
    <feature type="chain" id="PRO_0000036961" description="NTPase">
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            <begin position="340"/>
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    </feature>
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            <begin position="719"/>
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    </feature>
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        <location>
            <begin position="719"/>
            <end position="993"/>
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    </feature>
    <feature type="chain" id="PRO_0000341647" description="Protein p23/2">
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            <begin position="719"/>
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    </feature>
    <feature type="chain" id="PRO_0000341648" description="Protein p18">
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            <begin position="937"/>
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    </feature>
    <feature type="chain" id="PRO_0000036963" description="Viral genome-linked protein">
        <location>
            <begin position="994"/>
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        </location>
    </feature>
    <feature type="chain" id="PRO_0000036964" description="3C-like protease">
        <location>
            <begin position="1109"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000036965" description="RNA-directed RNA polymerase">
        <location>
            <begin position="1252"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000036967" description="Capsid protein VP60">
        <location>
            <begin position="1768"/>
            <end position="2344"/>
        </location>
    </feature>
    <feature type="domain" description="SF3 helicase" evidence="3">
        <location>
            <begin position="492"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C24" evidence="4">
        <location>
            <begin position="1109"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="domain" description="RdRp catalytic" evidence="2">
        <location>
            <begin position="1495"/>
            <end position="1619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1770"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="active site" description="For 3CLpro activity" evidence="4">
        <location>
            <position position="1135"/>
        </location>
    </feature>
    <feature type="active site" description="For 3CLpro activity" evidence="4">
        <location>
            <position position="1152"/>
        </location>
    </feature>
    <feature type="active site" description="For 3CLpro activity" evidence="4">
        <location>
            <position position="1212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="522"/>
            <end position="529"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by 3CLpro">
        <location>
            <begin position="143"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by 3CLpro">
        <location>
            <begin position="339"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by 3CLpro">
        <location>
            <begin position="718"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by host">
        <location>
            <begin position="936"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by 3CLpro">
        <location>
            <begin position="993"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by 3CLpro">
        <location>
            <begin position="1108"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by 3CLpro">
        <location>
            <begin position="1251"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by 3CLpro">
        <location>
            <begin position="1767"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(5'-phospho-RNA)-tyrosine" evidence="1">
        <location>
            <position position="1014"/>
        </location>
    </feature>
    <feature type="disulfide bond">
        <location>
            <begin position="1584"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_034381" description="In isoform Subgenomic capsid protein VP60." evidence="12">
        <location>
            <begin position="1"/>
            <end position="1765"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Complete loss of 3CLpro activity in vitro." evidence="10">
        <original>H</original>
        <variation>N</variation>
        <location>
            <position position="1135"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Partial loss of 3CLpro activity in vitro." evidence="10">
        <original>D</original>
        <variation>E</variation>
        <location>
            <position position="1152"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Complete loss of 3CLpro activity in vitro." evidence="10">
        <original>D</original>
        <variation>G</variation>
        <variation>N</variation>
        <location>
            <position position="1152"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Complete loss of 3CLpro activity in vitro." evidence="10">
        <original>D</original>
        <variation>G</variation>
        <variation>E</variation>
        <location>
            <position position="1175"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Partial loss of 3CLpro activity in vitro." evidence="10">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="1175"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Complete loss of 3CLpro activity in vitro." evidence="10">
        <original>C</original>
        <variation>G</variation>
        <location>
            <position position="1212"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Partial loss of 3CLpro activity in vitro." evidence="10">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="1212"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Complete loss of 3CLpro activity in vitro." evidence="10">
        <original>H</original>
        <variation>L</variation>
        <variation>N</variation>
        <location>
            <position position="1227"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="1257"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="1266"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="1282"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="1293"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="1300"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="1305"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="1315"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="1325"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="1337"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="turn" evidence="13">
        <location>
            <begin position="1354"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="1364"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="1373"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="1379"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="1385"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="1391"/>
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        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="1396"/>
            <end position="1410"/>
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    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="1418"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="1440"/>
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        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="1446"/>
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    </feature>
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        <location>
            <begin position="1466"/>
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        <location>
            <begin position="1470"/>
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        <location>
            <begin position="1482"/>
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        <location>
            <begin position="1495"/>
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        <location>
            <begin position="1505"/>
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    <feature type="helix" evidence="13">
        <location>
            <begin position="1511"/>
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        <location>
            <begin position="1529"/>
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        <location>
            <begin position="1543"/>
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        <location>
            <begin position="1547"/>
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        <location>
            <begin position="1564"/>
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    <feature type="helix" evidence="13">
        <location>
            <begin position="1594"/>
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        <location>
            <begin position="1600"/>
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    <feature type="strand" evidence="13">
        <location>
            <begin position="1606"/>
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    <feature type="helix" evidence="13">
        <location>
            <begin position="1613"/>
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    <feature type="helix" evidence="13">
        <location>
            <begin position="1619"/>
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        <location>
            <begin position="1633"/>
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        <location>
            <begin position="1647"/>
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        <location>
            <begin position="1655"/>
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    <feature type="strand" evidence="13">
        <location>
            <begin position="1663"/>
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        <location>
            <begin position="1670"/>
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        <location>
            <begin position="1679"/>
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        <location>
            <begin position="1698"/>
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        <location>
            <begin position="1716"/>
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        <location>
            <begin position="1730"/>
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        <location>
            <begin position="1740"/>
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    </feature>
    <evidence type="ECO:0000250" key="1"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00551"/>
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    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="10644847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="11040126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="8">
        <source>
            <dbReference type="PubMed" id="12504575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="9">
        <source>
            <dbReference type="PubMed" id="7474137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="10">
        <source>
            <dbReference type="PubMed" id="8083986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="11">
        <source>
            <dbReference type="PubMed" id="9557747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="12"/>
    <evidence type="ECO:0007829" key="13">
        <source>
            <dbReference type="PDB" id="1KHV"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="14">
        <source>
            <dbReference type="PDB" id="1KHW"/>
        </source>
    </evidence>
    <sequence length="2344" mass="257068" checksum="1454C248F81E9212" modified="1992-08-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>P27412</accession>
    <name>VP2_RHDVF</name>
    <protein>
        <recommendedName>
            <fullName>Protein VP2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Minor capsid protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">ORF2</name>
    </gene>
    <organism>
        <name type="scientific">Rabbit hemorrhagic disease virus (strain Rabbit/Germany/FRG/1989)</name>
        <name type="common">Ra/LV/RHDV/GH/1989/GE</name>
        <name type="synonym">RHDV-FRG</name>
        <dbReference type="NCBI Taxonomy" id="314536"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Pisuviricota</taxon>
            <taxon>Pisoniviricetes</taxon>
            <taxon>Picornavirales</taxon>
            <taxon>Caliciviridae</taxon>
            <taxon>Lagovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Oryctolagus cuniculus</name>
        <name type="common">Rabbit</name>
        <dbReference type="NCBI Taxonomy" id="9986"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1991" name="Virology" volume="184" first="664" last="676">
            <title>Rabbit hemorrhagic disease virus -- molecular cloning and nucleotide sequencing of a calicivirus genome.</title>
            <authorList>
                <person name="Meyers G."/>
                <person name="Wirblich C."/>
                <person name="Thiel H.-J."/>
            </authorList>
            <dbReference type="PubMed" id="1840711"/>
            <dbReference type="DOI" id="10.1016/0042-6822(91)90436-F"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Minor structural protein present in one or two copies per virion. Does not seem to play a role in capsid assembly, but is essential for production of infectious virus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">May interact with capsid protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Host cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>Translated by a ribosomal termination-reinitiation process from the bicistronic mRNA coding for VP1 and VP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lagovirus VP2 protein family.</text>
    </comment>
    <dbReference type="EMBL" id="M67473">
        <property type="protein sequence ID" value="AAA47286.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="B41039">
        <property type="entry name" value="VCWWRH"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_062876.1">
        <property type="nucleotide sequence ID" value="NC_001543.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1491967"/>
    <dbReference type="KEGG" id="vg:1491967"/>
    <dbReference type="Proteomes" id="UP000000413">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008558">
        <property type="entry name" value="Lagovirus_RHDV_Vp2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05801">
        <property type="entry name" value="DUF840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1035">Host cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000100128" description="Protein VP2">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="117" mass="12670" checksum="838811B8CAB21C46" modified="1992-08-01" version="1">MAFLMSEFIGLGLAGAGVLSNALLRRQELQLQKQAMENGLVLKADQLGRLGFNPNEVKNVIVGNSFSSNVRLSNMHNDASVVNAYNVYNPASNGIRKKIKSLNNSVKIYNTTGESSV</sequence>
</entry>
<copyright>
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