<?xml version="1.0" encoding="UTF-8"?>
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<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>O93058</accession>
    <accession>Q88561</accession>
    <name>RDRP_TMVOM</name>
    <protein>
        <recommendedName>
            <fullName>Replicase large subunit</fullName>
            <ecNumber>2.1.1.-</ecNumber>
            <ecNumber>2.7.7.-</ecNumber>
            <ecNumber>2.7.7.48</ecNumber>
            <ecNumber>3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>183 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>RNA-directed RNA polymerase</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Replicase small subunit</fullName>
                <ecNumber>2.1.1.-</ecNumber>
                <ecNumber>2.7.7.-</ecNumber>
                <ecNumber>3.6.4.13</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>126 kDa protein</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>Methyltransferase/RNA helicase</fullName>
                <shortName>MT/HEL</shortName>
            </alternativeName>
        </component>
    </protein>
    <organism>
        <name type="scientific">Tobacco mosaic virus (strain OM)</name>
        <name type="common">TMV</name>
        <dbReference type="NCBI Taxonomy" id="12251"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Kitrinoviricota</taxon>
            <taxon>Alsuviricetes</taxon>
            <taxon>Martellivirales</taxon>
            <taxon>Virgaviridae</taxon>
            <taxon>Tobamovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Nicotiana tabacum</name>
        <name type="common">Common tobacco</name>
        <dbReference type="NCBI Taxonomy" id="4097"/>
    </organismHost>
    <reference key="1">
        <citation type="submission" date="1995-12" db="EMBL/GenBank/DDBJ databases">
            <title>Nucleotide sequence of the coding region for 180K protein of tobacco mosaic virus common strain OM.</title>
            <authorList>
                <person name="Watanabe T."/>
                <person name="Hibi T."/>
                <person name="Ishihama A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="function">
        <molecule>Replicase large subunit</molecule>
        <text evidence="1">Is an RNA-dependent RNA polymerase active in viral RNA replication.</text>
    </comment>
    <comment type="function">
        <molecule>Replicase small subunit</molecule>
        <text evidence="1 5">Is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities (Potential). It also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May mediate silencing suppression through either inhibition of HEN1-mediated siRNA or siRNA demethylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a large and a small subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text>This protein is translated as a fusion protein by episodic readthrough of a termination codon. When readthrough of the amber terminator codon TAG occurs between the codons for Gln-1116 and Gln-1118, this results in the addition of the RdRp region to the replicase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ssRNA positive-strand viruses RNA-directed RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EC" id="2.7.7.-"/>
    <dbReference type="EC" id="2.7.7.48"/>
    <dbReference type="EC" id="3.6.4.13"/>
    <dbReference type="EMBL" id="D78608">
        <property type="protein sequence ID" value="BAA11429.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="EMBL" id="D78608">
        <property type="protein sequence ID" value="BAA11430.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PRIDE" id="O93058"/>
    <dbReference type="Proteomes" id="UP000000280">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008174">
        <property type="term" value="F:mRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039694">
        <property type="term" value="P:viral RNA genome replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027351">
        <property type="entry name" value="(+)RNA_virus_helicase_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002588">
        <property type="entry name" value="Alphavirus-like_MT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007094">
        <property type="entry name" value="RNA-dir_pol_PSvirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001788">
        <property type="entry name" value="Tymovirus_RNA-dep_RNA_pol"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00978">
        <property type="entry name" value="RdRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01443">
        <property type="entry name" value="Viral_helicase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01660">
        <property type="entry name" value="Vmethyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51743">
        <property type="entry name" value="ALPHAVIRUS_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51657">
        <property type="entry name" value="PSRV_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50507">
        <property type="entry name" value="RDRP_SSRNA_POS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1159">RNA suppression of termination</keyword>
    <keyword id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword id="KW-0941">Suppressor of RNA silencing</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0693">Viral RNA replication</keyword>
    <feature type="chain" id="PRO_0000041168" description="Replicase large subunit">
        <location>
            <begin position="1"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000041169" description="Replicase small subunit">
        <location>
            <begin position="1"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="domain" description="Alphavirus-like MT" evidence="4">
        <location>
            <begin position="72"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="(+)RNA virus helicase ATP-binding">
        <location>
            <begin position="801"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="(+)RNA virus helicase C-terminal">
        <location>
            <begin position="964"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="domain" description="RdRp catalytic" evidence="3">
        <location>
            <begin position="1380"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Methyltransferase" evidence="2">
        <location>
            <begin position="50"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Helicase" evidence="2">
        <location>
            <begin position="830"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="833"/>
            <end position="840"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="1616" mass="183332" checksum="1D4E01831E24BDBB" modified="1998-11-01" version="1"> </sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>