<?xml version="1.0" encoding="UTF-8"?>
<uniprot xmlns="http://uniprot.org/uniprot"
 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
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<entry dataset="Swiss-Prot" created="1993-07-01" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q02004</accession>
    <name>GP_DUGBA</name>
    <protein>
        <recommendedName>
            <fullName>Envelopment polyprotein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>M polyprotein</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Mucin-like variable region</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="2">GP38</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="2">Glycoprotein N</fullName>
                <shortName>Gn</shortName>
            </recommendedName>
            <alternativeName>
                <fullName>Glycoprotein G2</fullName>
            </alternativeName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="2">Non-Structural protein M</fullName>
                <shortName>NSm</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="2">Glycoprotein C</fullName>
                <shortName>Gc</shortName>
            </recommendedName>
            <alternativeName>
                <fullName>Glycoprotein G1</fullName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name type="primary">GP</name>
    </gene>
    <organism>
        <name type="scientific">Dugbe virus (isolate ArD44313)</name>
        <name type="common">DUGV</name>
        <dbReference type="NCBI Taxonomy" id="766194"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Ellioviricetes</taxon>
            <taxon>Bunyavirales</taxon>
            <taxon>Nairoviridae</taxon>
            <taxon>Orthonairovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Amblyomma variegatum</name>
        <name type="common">Tropical bont tick</name>
        <dbReference type="NCBI Taxonomy" id="34610"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Rhipicephalus annulatus</name>
        <dbReference type="NCBI Taxonomy" id="34611"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Rhipicephalus</name>
        <dbReference type="NCBI Taxonomy" id="34630"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Rhipicephalus decoloratus</name>
        <name type="common">African blue tick</name>
        <name type="synonym">Boophilus decoloratus</name>
        <dbReference type="NCBI Taxonomy" id="60189"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Hyalomma truncatum</name>
        <dbReference type="NCBI Taxonomy" id="72855"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Hyalomma rufipes</name>
        <name type="common">Tick</name>
        <name type="synonym">Hyalomma marginatum rufipes</name>
        <dbReference type="NCBI Taxonomy" id="72862"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Rhipicephalus geigyi</name>
        <dbReference type="NCBI Taxonomy" id="136141"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1992" name="Virology" volume="190" first="606" last="615">
            <title>Dugbe Nairovirus M RNA: nucleotide sequence and coding strategy.</title>
            <authorList>
                <person name="Marriott A.C."/>
                <person name="El-Ghorr A.A."/>
                <person name="Nuttall P.A."/>
            </authorList>
            <dbReference type="PubMed" id="1387749"/>
            <dbReference type="DOI" id="10.1016/0042-6822(92)90898-Y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
        <scope>PROTEIN SEQUENCE OF 897-905</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Glycoprotein C and glycoprotein N interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Glycoprotein C and Glycoprotein N interact with each other.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Glycoprotein C</molecule>
        <subcellularLocation>
            <location evidence="5">Virion membrane</location>
            <topology evidence="5">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Host Golgi apparatus membrane</location>
            <topology evidence="5">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Host endoplasmic reticulum membrane</location>
            <topology evidence="5">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">Interaction between Glycoprotein C and Glycoprotein N is essential for proper targeting of Glycoprotein C to the Golgi complex, where virion budding occurs.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Glycoprotein N</molecule>
        <subcellularLocation>
            <location evidence="5">Virion membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Host Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text>Specific enzymatic cleavages in vivo yield mature proteins including Glycoprotein C and Glycoprotein N.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the nairovirus envelope glycoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="M94133">
        <property type="protein sequence ID" value="AAA42974.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PIR" id="A43364">
        <property type="entry name" value="A43364"/>
    </dbReference>
    <dbReference type="SMR" id="Q02004"/>
    <dbReference type="PRIDE" id="Q02004"/>
    <dbReference type="Proteomes" id="UP000000278">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044167">
        <property type="term" value="C:host cell endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044178">
        <property type="term" value="C:host cell Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055036">
        <property type="term" value="C:virion membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039654">
        <property type="term" value="P:fusion of virus membrane with host endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046718">
        <property type="term" value="P:viral entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019062">
        <property type="term" value="P:virion attachment to host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002532">
        <property type="entry name" value="Hanta_Gc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012487">
        <property type="entry name" value="Nairovirus_M"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01561">
        <property type="entry name" value="Hanta_G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07948">
        <property type="entry name" value="Nairovirus_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1170">Fusion of virus membrane with host endosomal membrane</keyword>
    <keyword id="KW-1168">Fusion of virus membrane with host membrane</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1038">Host endoplasmic reticulum</keyword>
    <keyword id="KW-1040">Host Golgi apparatus</keyword>
    <keyword id="KW-1043">Host membrane</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-1161">Viral attachment to host cell</keyword>
    <keyword id="KW-1162">Viral penetration into host cytoplasm</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000036802" description="Envelopment polyprotein">
        <location>
            <begin position="18"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000369248" description="Mucin-like variable region">
        <location>
            <begin position="18"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000434912" description="GP38" evidence="2">
        <location>
            <begin position="96"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000036804" description="Glycoprotein N" evidence="3">
        <location>
            <begin position="371"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000434913" description="Non-Structural protein M" evidence="2">
        <location>
            <begin position="698"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000036805" description="Glycoprotein C" evidence="3">
        <location>
            <begin position="894"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="547"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="676"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="705"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="824"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1452"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="24"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by host" evidence="1">
        <location>
            <begin position="370"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by host signal peptidase" evidence="1">
        <location>
            <begin position="893"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="848"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="1201"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="1258"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; by host" evidence="3">
        <location>
            <position position="1420"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8JSZ3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="1551" mass="173355" checksum="7C1654C63895C620" modified="1993-07-01" version="1" precursor="true">MSKRVLIIAVVVYLVFTTQNQITGNHTTINSSSPSTTEASSTPTVSRTPQTTTTSTAVSTTITATTTPTASWTTQSQYFNKTTQHHWREETMISRNPTVLDRQSRASSVRELLNTKFLMLLGFIPKGEVNHLENACNREGKNCTELILKERIARFFSETEKESCYNTYLEKHLRSVSPEVSLTPYRVLGLREDILLKEIDRRIIRFETDSQRVTCLSASLLKPDVFIREQRIDAKPSNGPKIVPVDSVACMNLEANVDVRSNKLVIQSLMTTVKISLKNCKVVVNSRQCIHQQTGSGVIKVPKFEKQQGGTWSSYIAGVYTATIDLLDENNQNCKLFTECIVKGRELVKGQSELKSFNIEVLLPRVMKTRRKLLAVTDGSTECNSGTQLIEGKSIEVHKQDIGGPGKKLTICNGTSVLDVPLDEGHGCYTINVITSKRACRPKNSKLQCSIDKELKPCDSGKCLSISQKGAGHIKVSRGKTILITECKEHCQIPVPTGKGDIMVDCSGGRQHYLEVNIVDIHCPNTKFLGGIMLYFCRMSSRPTVALLLGIWIGCGYILTCIFSFLLYHLILFFANCIKQCRKKGERLGEICVKCEQQTVNLMDQELHDLNCNFNLCPYCCNRMSDEGMSRHVGKCPKRLERLNEIELYLTTSECLCLSVCYQLLISVGIFLKRTTWLVVLLVLLGLAISPVQGAPTEVSNVKQDGDYSICYFIFGCLVTAALLLKVKRTNSNGIVVVVDSFGRCPYCNEFTDSLFEEVLHDTLCSLCVCPFCEKQALDLVTLEEHVKECYKVATRKDIFKILGRKFTNALVRREKLFTTGLQLFINKTNVVVFALIMCFLLLLTGHNASAFDSGDLPDGVWEESSQLVKSCTQFCYIEEDVCYCPAEDGVGRKLLFFNGLQNSVKRLSDSHKLLTSVSIDAPWGRINVESTWKPTLAASNIAMSWSSTDIKGEKVILSGRSTSIIKLKEKTGVMWKLVGSGLASEKKKPFRFPIMDFAQVYNSVFQYITGDRLLSEWPKAVCTGDCPHRCGCQTSTCMAKECHTQECVSTHMVLGIGTGCTCCGMDVERPFNKYLGVKWSTEYLRTEVLVCVEVTEEERHCEIVEAGTRFNIGPITITISDPQNIGSKLPESLMTVQEIDDSNFVDIMHVGNVISADNSCRLQSCTHGSAVTTRFTALTALIKDDHSSGLNLAVLDPKVNSSWLSWEGCDMDYYCNVGDWPTCTYTGVVTQKLREFLKLDQHRKRLHTTLSFSLKKNLSKRSHTSVRLEGKTVTRMEVKVTALIEVDGMELHSKTIRLSGIRLTGLKCSGCFSCTSGISCSVNAKLTSPDEFTLHLRSTSPNVVVAETSIIARKGPSATTSRFKVFSVRDTKKICFEVVEREYCKDCTPDELTTCTGVELEPTKDILLEHRGTIVQHQNDTCKSKIDCWSNSISSFASGIGDFFKHYIGSIAVGVLGTVLPFALLILFFIYGDKMLWPFKVFCRPCRRCCRKNEGYNKLAEEEELRDIIRKFSKSGELINKDAKDKRTLARLFMSDNPKLKKEKKLSEIA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q66431</accession>
    <name>L_DUGBA</name>
    <protein>
        <recommendedName>
            <fullName>RNA-directed RNA polymerase L</fullName>
            <shortName>Protein L</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Large structural protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Replicase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Transcriptase</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName>Ubiquitinyl hydrolase</fullName>
                <ecNumber evidence="7">3.4.19.12</ecNumber>
                <ecNumber evidence="7">3.4.22.-</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName>cap-snatching endonuclease</fullName>
                <ecNumber evidence="2">3.1.-.-</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName>RNA-directed RNA polymerase</fullName>
                <ecNumber>2.7.7.48</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name type="primary">L</name>
    </gene>
    <organism>
        <name type="scientific">Dugbe virus (isolate ArD44313)</name>
        <name type="common">DUGV</name>
        <dbReference type="NCBI Taxonomy" id="766194"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Ellioviricetes</taxon>
            <taxon>Bunyavirales</taxon>
            <taxon>Nairoviridae</taxon>
            <taxon>Orthonairovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Amblyomma variegatum</name>
        <name type="common">Tropical bont tick</name>
        <dbReference type="NCBI Taxonomy" id="34610"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Rhipicephalus annulatus</name>
        <dbReference type="NCBI Taxonomy" id="34611"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Rhipicephalus</name>
        <dbReference type="NCBI Taxonomy" id="34630"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Rhipicephalus decoloratus</name>
        <name type="common">African blue tick</name>
        <name type="synonym">Boophilus decoloratus</name>
        <dbReference type="NCBI Taxonomy" id="60189"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Hyalomma truncatum</name>
        <dbReference type="NCBI Taxonomy" id="72855"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Hyalomma rufipes</name>
        <name type="common">Tick</name>
        <name type="synonym">Hyalomma marginatum rufipes</name>
        <dbReference type="NCBI Taxonomy" id="72862"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Rhipicephalus geigyi</name>
        <dbReference type="NCBI Taxonomy" id="136141"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="1996" name="J. Gen. Virol." volume="77" first="1775" last="1780">
            <title>Large RNA segment of Dugbe nairovirus encodes the putative RNA polymerase.</title>
            <authorList>
                <person name="Marriott A.C."/>
                <person name="Nuttall P.A."/>
            </authorList>
            <dbReference type="PubMed" id="8760425"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="Crit. Rev. Microbiol." volume="43" first="753" last="778">
            <title>Bunyaviridae RdRps: structure, motifs, and RNA synthesis machinery.</title>
            <authorList>
                <person name="Amroun A."/>
                <person name="Priet S."/>
                <person name="de Lamballerie X."/>
                <person name="Querat G."/>
            </authorList>
            <dbReference type="PubMed" id="28418734"/>
            <dbReference type="DOI" id="10.1080/1040841x.2017.1307805"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="journal article" date="2013" name="J. Virol." volume="87" first="3815" last="3827">
            <title>Diversity of ubiquitin and ISG15 specificity among nairoviruses' viral ovarian tumor domain proteases.</title>
            <authorList>
                <person name="Capodagli G.C."/>
                <person name="Deaton M.K."/>
                <person name="Baker E.A."/>
                <person name="Lumpkin R.J."/>
                <person name="Pegan S.D."/>
            </authorList>
            <dbReference type="PubMed" id="23345508"/>
            <dbReference type="DOI" id="10.1128/jvi.03252-12"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 2-169 IN COMPLEX WITH UBIQUITIN</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>FUNCTION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>INTERACTION WITH HOST UBIQUITIN</scope>
        <scope>MUTAGENESIS OF GLU-10; GLY-101 AND THR-128</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3 7">Displays RNA-directed RNA polymerase, deubiquitinating and deISGylase activities (PubMed:23345508). RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome (By similarity). During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription (By similarity). The deubiquitinating and deISGylating activities specifically cleaves poly-ubiquitinated conjugates and ISG15 from RIG-I, interfering with antiviral signaling pathways mediated by NF-kappaB and IRF signalings (By similarity). Favors K48 poly-Ub linkages (PubMed:23345508).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="7">35.7 uM for Ub-AMC</KM>
            <KM evidence="7">5.21 uM for ISG15-AMC</KM>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Interacts (via N-terminus) with host ISG15 (via C-terminus); the deISGylase activity of the viral protein interferes with antiviral signaling pathways mediated by NF-kappaB and IRF signalings (By similarity). Interacts with host ubiquitin (PubMed:23345508).</text>
    </comment>
    <comment type="domain">
        <text evidence="3">The viral OTU domain (vOTU) and its N-terminal extension is responsible for ubiquitin and ISG15 binding and for the deubiquitination and deISGylation activities.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Classified as His(+) endonuclease since it has a histidine upstream of the active site that coordinates the first cation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Bunyavirales RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EC" id="3.4.22.-" evidence="7"/>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EC" id="2.7.7.48"/>
    <dbReference type="EMBL" id="U15018">
        <property type="protein sequence ID" value="AAB18834.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="PDB" id="4HXD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.85 A"/>
        <property type="chains" value="B/D=2-169"/>
    </dbReference>
    <dbReference type="PDBsum" id="4HXD"/>
    <dbReference type="SMR" id="Q66431"/>
    <dbReference type="MEROPS" id="C87.001"/>
    <dbReference type="PRIDE" id="Q66431"/>
    <dbReference type="Proteomes" id="UP000000278">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039648">
        <property type="term" value="P:modulation by virus of host protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039689">
        <property type="term" value="P:negative stranded viral RNA replication"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039696">
        <property type="term" value="P:RNA-templated viral transcription"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039579">
        <property type="term" value="P:suppression by virus of host ISG15-protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039502">
        <property type="term" value="P:suppression by virus of host type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007099">
        <property type="entry name" value="RNA-dir_pol_NSvirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007322">
        <property type="entry name" value="RNA_pol_bunyavir"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04196">
        <property type="entry name" value="Bunya_RdRp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50525">
        <property type="entry name" value="RDRP_SSRNA_NEG_SEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0945">Host-virus interaction</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword id="KW-1114">Inhibition of host interferon signaling pathway by virus</keyword>
    <keyword id="KW-1095">Inhibition of host ISG15 by virus</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1127">Modulation of host ubiquitin pathway by viral deubiquitinase</keyword>
    <keyword id="KW-1130">Modulation of host ubiquitin pathway by virus</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0899">Viral immunoevasion</keyword>
    <keyword id="KW-0693">Viral RNA replication</keyword>
    <feature type="chain" id="PRO_0000222026" description="RNA-directed RNA polymerase L">
        <location>
            <begin position="1"/>
            <end position="4036"/>
        </location>
    </feature>
    <feature type="domain" description="OTU" evidence="4">
        <location>
            <begin position="29"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="RdRp catalytic" evidence="5">
        <location>
            <begin position="2424"/>
            <end position="2639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Essential for the vOTU enzymatic activity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Endonuclease" evidence="2">
        <location>
            <begin position="624"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="779"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2800"/>
            <end position="2833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="4008"/>
            <end position="4036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="793"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="828"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="847"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="4016"/>
            <end position="4036"/>
        </location>
    </feature>
    <feature type="active site" description="For ubiquitin thioesterase activity" evidence="3">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" description="For ubiquitin thioesterase activity" evidence="3">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" description="For ubiquitin thioesterase activity" evidence="3">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="For endonuclease activity" evidence="1">
        <location>
            <position position="733"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="631"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="692"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="692"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="717"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="2606"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic; for RdRp activity</note>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="75% loss of debubiquitination activity and 60% loss of deISGylation activity." evidence="7">
        <original>E</original>
        <variation>T</variation>
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="More than 90% loss of deISGylation and debubiquitination activities." evidence="7">
        <original>G</original>
        <variation>S</variation>
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="110% increase in debubiquitination activity and almost 2,000% increase in deISGylation activity." evidence="7">
        <original>T</original>
        <variation>E</variation>
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="3"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="25"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="29"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="36"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="50"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="55"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="73"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="77"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="86"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="116"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="141"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="152"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="I0DF35"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q6GWS6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q6TQR6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="23345508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="8"/>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="4HXD"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="4HXD"/>
        </source>
    </evidence>
    <sequence length="4036" mass="459392" checksum="E2EDF0B4358E31BD" modified="1996-11-01" version="1">MDFLDSLIWERVVDEQYITNPTFCVSDYFEVIRQPGDGNCFYHSIAELFFDVKTPSSFRKVKEHLQLAAEVYYDTEPEAVGTGISKDEYIKVAMKDNEWGGSLEASMLSKHLQTTIILWVVNSTEQVTAAIKFGPGRVSTALNLMHVGRTHFDALRIIEQLENNQPQDRNRLDIADRIAAAEVYVRQSIEDNLQEDEFFDYAREDEISEDVSAPGGSREATELKKKAILLNKTVKRGENIPIRVGRVLDCLFSCKIAVSLDEGLLYLRPETRESEATSISLRQLGHKLLTRDRHIKMEYARSKLYVTRDLIDHLDIGGLLRSSFPGLGLERYIQLLHSELVLDLVTVVLAVLLSTFLYGSNNKNKKQFITNCLLNTKLSGKRVFKALSKLTGQMLYRTPKRAVSIVSQELYGKLMLKVKNNLEGMGPISMLALRNLNFDNMQLQDYLEMLSEMSKIDNSDVEYTHREISDLHTLVERLSKLQKSQDVNELKLWFKEEVLTKRSQRSVGNAFEFLINDYFKKKDIMKFVSTSGKASSTGNIGNVLSYAHNLYLSKESLRMTSEDVTQLLIEIRKLHKLQGDLSIEPVAIICDKLEDQFRKLFRELPEECSSECQTLFNDIRNSPSHSVAWKHALRLKGTAYEGMFAKQYGWSYISEDIKPSLTMIVQTLFPESFEAFLDRTQLHPEFRDLTPDYALTQKIFFPRNTIPRTENRQLAIDVSLEGSVEAVPIVEKRMFPLPEVPIGEANSISRVMNIFKEKREESMQKKLEHDRQAEANRLKSAGLSASKAEQEVCNSAQDRKEEKERTTEPAGKQQRTEDLVVIEGNQDEGDSDPQKKVDEKTVPGESKQHSKSSGSSSTNQMSQKVVDVPSVEDSSDQAPGDFPDYGYYFKRIVMDESGTVLTEEAQLEKRQLLFIEVGYQTDVDGKITTDYKKWKDILRLLELLNIKCSFIACADCSSTPSNNWWISEDKVRLLKNSISHLFSKLTQNSPADVTDIVVGSISTQKVRSYLKSGTATKTPISLKDVQETWSKMKDYIVNRPTGISLNKELVGALYQGLVEGAIISKEGTVNLIQMLKDKQERITDEFERTKLKHEVNEDVKTSEKLLLGWLMEDLKGCRCMGCLTKIKELSESMSVNQDRLEYLSTNCQTKSHCTECHPRSLEYRNISNVDNRVPSMQRVSHSRNEGFEDTNETLTELDRLVRLTLPGKTEKERRVKRNVEGLIRFMMQQSSLDCIKLPSGQIIAHRCSKKFKNSSEAEEKCNERFERLMKELSEQKLKPYSDHVRKTITSSLKKTDKQAGSKCAVPRLWLETLIRDLRVPTKDEEILLNIRTSMQSKTNFIRNNDKLIIRSNKEIADYLETKRKNLLSEKASDKIFSSDCILFKEVIAEALRRYYSTPYEGVPETIVKLINFLCTFGWFQEVVLYSKICETFLRCCTEFSRSGIKLVKVRHCDTNLSIKLPSNKKENMLCCLYDKNMSLLKGPFFLNRRQAILGSAYPYILITLYIQVLQQHRCLEVLNSVNDRVVGNINTCTSNLLNTVKAELTLVNSGLFEKAYECRTEQCRLGGNFLNRSSRDHFISTVSGLNVVYGALIKDNLLANSQPQNKQLQMLRFGMLCGLSRLSSALELGKKFSTSCRRIEDNIMRLYLQSTIYSANRDVSQNVQNWKMKDLCPDITIPCFSVYGLFVNSDRQRIFDIYNVHIYNKEMDNFDEGCITVLEETAERHMLWELDLLRSLEGDTRDVRAARLLLGCPNIRKATDKDGNRLMKKGITDDWREEAGSDSSSISGRRSYASSGTRVKSMFGKYNSSQKPFELKPGLEVVNDPLHDYKQAVQDSFCYSEYTPNTESVLKDCIHIIRTNPSHTMGSYELIQAVTENARRKYPPENIERARKDPKNWVSISEVTETTSIVSQPRTHFMLKDCYKVLLGTENKKIVKMLRGKLKKLGAMRTDIEIGKKDCLDLLTTVDGLSEEQCKNIVNGIFEPSKLSFYHWKDLLKKELSEVLLTDDGNYIYCWLKTLSSMVKHSLKKDLRFMTGKNSLDIKPEMFTDEEYSALNIMKLELLGEHTDGIQGKTDFLLSSWKKCALKPKEGQSILNVGLNSLAALHDELYDIRLQHLELTRIKKENPTVSFTKEEILVKRLEKGFLNKYKKEVMEAVNLIFYCCLTAPWCLHYKSLEAYLVRHPEILETECIKENDIPLLDLTVTSLIRSLIDDIEGESSFNDSSDIKVRFAVKYLITLFTANGEPFSLSLNDGGLNDDLQLTTDEKLLYQTKKVFAKLGLSGNNYDFIWTLQMIANSNFNVCKRLTGRTTGERLPRSVRSKVIYEMVKLVGETGMAILQQLAFSQALNYDHRFYAVLAPKAQLGGSRDLLVQETGTKVIHATTEMFSRNLLKTTSDDGLTNPHLKETRLNIGLDMLSTARALDGKQVSDDSNLLNFFKTVCISGDNTKWGPIHCCSFFSGMMQQLLKDVQDWSSFYKLTFIKNLCRQIEIPAPSIRKILNVLRFKLSDKGGVEKLSEEAIRSELINNLAEWEGNDTVKFLITTYISKGIMAMNSYNHMGQGIHHATSSLLTSMMAETFEELAVDYMKKHFPGLTVNVDHAGSSDDYAKCIIVSGLVSKDMYKRYDGVFWRHMCRLKNFLAAVRRCCQMKDSAKTLVGDCFLEFYSEFMMGNRVTPAVIKFIFTGLINSSVTSPQSLVQACHVSSQQGMYNSVPLVTNAAFTILRQQIFYNHVEDFIRRYGLITLGAVSPFGRLFLPRFSGLVSSSVALEDSETISKAAAEINSNDIFFNTSSLSNLDKLEQSPDSSGLDDDSVVSTTTVESSDSKGSSSSFTFDLNRPLSETEVKFLKLLRELTSTTACEMLQEKINTLYNDSREGPLDRHNILQNCRLSESCDWLLDGKKRGLLELSRRMSCLLNVLIAGYYRSFGSEGTEKQVKASWSRDDNRVIEDPMIQLIPEKLRRELERLGLSRMEVDELMPAVGPDESLSQLVAKKLISLNVSTEEYSAEVSRLKQTLTARNVLHGLAGGIKELSLPIYTIFMKPYFFKDNVFLDLEDRWSSRHSTNYRDSTGKMLTGKVVTKFTHWLDTFLSCVVSANRSQEIKECSLFNPNLRCVNIMVKGNGIKELSYIRSHLSVLSVEFENLNLQFSDVNRQKLKIVESRPPECELEANKAVIIKSKLFSAVEHVRLSNNPAVVMGYLLEESSISEVKPTKVDFSNLLKHRFKLMQFFPSVFTLLRSLQSESKELEKLGEPVDMHQVSKYANHLTLLCRMIQQSKPSLTVFYMLKGSQMNTEPTVSELVSYGIKEGRFLKLPEIGLDASTYSVRYWKILHCISAIGELPLSSEDKTSLLISFLNWKVTSDCVDDCCPLEKYDKAIVSEFSGQVLINTLASELSSVRKDQEREGLTDLIDYINSPSELLKKKPYLGTTCRFQCWGEGAKSGKFTYSSRTGEAIGIFVAGKLHIHLTSDSPGLLCEVERQVLSWLGKRRTDVLTKEQHQFFLDFLPNLSEVVQKNRDGAILGVTIDSTNVRMLKYVPPKRNTPVIKIKKQILTVKKQTTLDVESEPRIVWGHGQLSIVYDECETETTYHENLIKVKKLVDLASGTTDKLPTAIFSDTRITLARVKFKTELLLNSLCLLHCFLKHTSQDAIQEVESKCNVLERYLRSGGVQFRPMSESLDKKVTKLPLQCQSDKDVDKEINFCEDLTRVFSNENVPLSSWSEVQSYIEEVGFGNVLVHIEKNPTRSDLIWRFSIDSISGNFGPIKDIRTLVTYMSTETVPKFLLPFLLFEEQLKHLIAGCVELRDALNSSGINDREIAIVALFTCFYYQSDSVKRQGPVCSISSFCSLIGDDLLPLDNRLQARVLPEQDNVKLHFKLNLTTDSALGKKDKAIQAKKIISRYLRLIFTEDDMDLKRLKSDATKVKLSSEKECEFLEFCLHSDLSYALNYRVLLEHLIDLEDRAKKTACVLIEEFILMLTGRLMISSTIDSDSKRTLEDDALCLEDLLDSDNEASSSKSDNEEQIALQTGKFNFNWDSD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-07-13" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8V336</accession>
    <name>NCAP_DUGBA</name>
    <protein>
        <recommendedName>
            <fullName>Nucleoprotein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Nucleocapsid protein</fullName>
            <shortName>Protein N</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">N</name>
    </gene>
    <organism>
        <name type="scientific">Dugbe virus (isolate ArD44313)</name>
        <name type="common">DUGV</name>
        <dbReference type="NCBI Taxonomy" id="766194"/>
        <lineage>
            <taxon>Viruses</taxon>
            <taxon>Riboviria</taxon>
            <taxon>Orthornavirae</taxon>
            <taxon>Negarnaviricota</taxon>
            <taxon>Polyploviricotina</taxon>
            <taxon>Ellioviricetes</taxon>
            <taxon>Bunyavirales</taxon>
            <taxon>Nairoviridae</taxon>
            <taxon>Orthonairovirus</taxon>
        </lineage>
    </organism>
    <organismHost>
        <name type="scientific">Homo sapiens</name>
        <name type="common">Human</name>
        <dbReference type="NCBI Taxonomy" id="9606"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Amblyomma variegatum</name>
        <name type="common">Tropical bont tick</name>
        <dbReference type="NCBI Taxonomy" id="34610"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Rhipicephalus annulatus</name>
        <dbReference type="NCBI Taxonomy" id="34611"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Rhipicephalus</name>
        <dbReference type="NCBI Taxonomy" id="34630"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Rhipicephalus decoloratus</name>
        <name type="common">African blue tick</name>
        <name type="synonym">Boophilus decoloratus</name>
        <dbReference type="NCBI Taxonomy" id="60189"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Hyalomma truncatum</name>
        <dbReference type="NCBI Taxonomy" id="72855"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Hyalomma rufipes</name>
        <name type="common">Tick</name>
        <name type="synonym">Hyalomma marginatum rufipes</name>
        <dbReference type="NCBI Taxonomy" id="72862"/>
    </organismHost>
    <organismHost>
        <name type="scientific">Rhipicephalus geigyi</name>
        <dbReference type="NCBI Taxonomy" id="136141"/>
    </organismHost>
    <reference key="1">
        <citation type="journal article" date="2002" name="Virology" volume="294" first="364" last="371">
            <title>Dugbe nairovirus S segment: correction of published sequence and comparison of five isolates.</title>
            <authorList>
                <person name="Bridgen A."/>
                <person name="Dalrymple D.A."/>
                <person name="Elliott R.M."/>
            </authorList>
            <dbReference type="PubMed" id="12009878"/>
            <dbReference type="DOI" id="10.1006/viro.2001.1324"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC RNA]</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Virion</location>
        </subcellularLocation>
        <text>Internal protein of virus particle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nairovirus nucleocapsid protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AF434161">
        <property type="protein sequence ID" value="AAL73396.1"/>
        <property type="molecule type" value="Genomic_RNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q8V336"/>
    <dbReference type="Proteomes" id="UP000000278">
        <property type="component" value="Genome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019029">
        <property type="term" value="C:helical viral capsid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019013">
        <property type="term" value="C:viral nucleocapsid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003486">
        <property type="entry name" value="Nairo_nucleocap"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02477">
        <property type="entry name" value="Nairo_nucleo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003950">
        <property type="entry name" value="N_NairoV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0167">Capsid protein</keyword>
    <keyword id="KW-1139">Helical capsid protein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0543">Viral nucleoprotein</keyword>
    <keyword id="KW-0946">Virion</keyword>
    <feature type="chain" id="PRO_0000395843" description="Nucleoprotein">
        <location>
            <begin position="1"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="483" mass="53917" checksum="09ED38AE453118C1" modified="2002-03-01" version="1">MENQIKANNKKEFDEWFKPFSEKLQLRSNLTNSASLCDRVPDLALAEMKMALATDDKEKDSIFSNALVEATRFCAPIYECAWTCSTGVVQKSLSWFDKNKDFIKLWDAKYMDLKKGIPEPEQLVSYQQAAQKWRKDVGYEINQFTRSLTHPVVAEYKVPGEIAVDVKEMLSDMIRRRNVLLNGDGENAGKKGPISREHVSWGRELAGGKFQVVFNPPWGDINKCGKSGIPLAATAMVKVAELDGSKKLEDIRQALLDLKKWVEDNKDALEDGKGNELVQTMTKHLAQAVELSKKSNALRAQGAQIDTPFSAYYWAWSAGVKPETFFTLSQFLFEMGQSARGGKKMIKALTSTPLRWGKGLINLFADDDFLGNRLYMHPAVLTPGRMSEMGACFGVIPVASPEDAILGSGHSKNILNFKIDTSVQNPCASTIVQLYRIQKSGFDLESLEVVSTEHLLHQSFVGKRCPTQNAYKVRGNATNVNII</sequence>
</entry>
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Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>