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    <name>Q3YQN1_EHRCJ</name>
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        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
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                <person name="Mavromatis K."/>
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                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
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                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
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                <person name="Kyrpides N.C."/>
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            <dbReference type="PubMed" id="16707693"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="101" mass="11342" checksum="AB7D63544878679B" modified="2005-09-27" version="1">MYSSDQLSSLQEMLKKKFSDFQEHQKPQIFEGSSLGGKVSVKINVSNMVSYQVTEVKLDPSLLQEKAILIEDLIRAAFNDALKKSSDYNKNLISSLLPFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQN8</accession>
    <name>Q3YQN8_EHRCJ</name>
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        <name type="scientific">Ehrlichia canis (strain Jake)</name>
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            <taxon>Bacteria</taxon>
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            <taxon>Alphaproteobacteria</taxon>
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            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
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                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TsaE family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAZ68967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0936"/>
    <dbReference type="EnsemblBacteria" id="AAZ68967">
        <property type="protein sequence ID" value="AAZ68967"/>
        <property type="gene ID" value="Ecaj_0936"/>
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    <dbReference type="KEGG" id="ecn:Ecaj_0936"/>
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    <dbReference type="Proteomes" id="UP000000435">
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="T6A_TsaE"/>
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        <property type="entry name" value="SSF52540"/>
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    <dbReference type="TIGRFAMs" id="TIGR00150">
        <property type="entry name" value="T6A_YjeE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="155" mass="17830" checksum="8489459D55DE08DA" modified="2005-09-27" version="1">MQFKYNSVDLVFLEKLAHFVALNLKKCDSVSLVGDLGVGKTAFVRFLVNTLIPSEDVSSPTFSIINEYHSSEFIIYHVDLYRINSLSEVYDLGLDCICNNGICIVEWPNLLDSILNFNLRININCSIRENLRDIEVITNDSDWYNAFKKFIHQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQN9</accession>
    <name>Q3YQN9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Bacterial translation initiation factor 3 (BIF-3)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0935</name>
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    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IF-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0935"/>
    <dbReference type="EnsemblBacteria" id="AAZ68966">
        <property type="protein sequence ID" value="AAZ68966"/>
        <property type="gene ID" value="Ecaj_0935"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0935"/>
    <dbReference type="eggNOG" id="COG0290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054919_3_2_5"/>
    <dbReference type="OMA" id="RNMIMFL"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036788">
        <property type="entry name" value="T_IF-3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036787">
        <property type="entry name" value="T_IF-3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001288">
        <property type="entry name" value="Translation_initiation_fac_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019815">
        <property type="entry name" value="Translation_initiation_fac_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019814">
        <property type="entry name" value="Translation_initiation_fac_3_N"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00168">
        <property type="entry name" value="infC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF3_N" evidence="5">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="IF3_C" evidence="4">
        <location>
            <begin position="75"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="159" mass="18405" checksum="B7F99FAF8E6E2D1E" modified="2005-09-27" version="1">MITEKKVKLVDQNSVMIGVVDIEDALSRAKAVNLDLVEIVHDDEYPLCKIFDYSKYRYSHKKKISDSKKKQKTIIVKELKFKLNIGDNDYNVKLNMLRSFIERGDKVKISLRFIGREILHPEVGMEIIERLIKDTSDIAKPENLLKKEGNLINMVLTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQR1</accession>
    <name>Q3YQR1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0833</name>
        <name evidence="2" type="ordered locus">Ecaj_0913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-07" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Ehrlichia canis str. Jake.</title>
            <authorList>
                <person name="Palenik B."/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Mavrommatis K."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304941.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68864">
        <property type="protein sequence ID" value="AAZ68864"/>
        <property type="gene ID" value="Ecaj_0833"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68944">
        <property type="protein sequence ID" value="AAZ68944"/>
        <property type="gene ID" value="Ecaj_0913"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0833"/>
    <dbReference type="KEGG" id="ecn:Ecaj_0913"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="287" mass="31988" checksum="9DFD4D01D3EE413F" modified="2005-09-27" version="1">MKYKKTFTVTALVLLTSFTHFIPFYSPARASTIHNFYISGKYMPTASHFGIFSAKEEQSFTKVLVGLDQRLSHNSINNNDTAKSLKVQNYSFKYKNNPFLGFAGAIGYSIGNSRIELEVSHEIFDTKNPGNNYLNDSHKYCALSHGSHICSDGNSGDWYTAKTDKFVLLKNEGLLDVSFMLNACYDITTEKMPFSPYICAGIGTDLISMFETTQNKISYQGKLGLNYTINSRVSVFAGGHFHKVIGNEFKGIPTLLPDGSNIKVQQSATVTLDVCHFGLEIGSRFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQT2</accession>
    <name>Q3YQT2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Polynucleotide adenylyl transferase</fullName>
            <ecNumber evidence="6">2.7.7.72</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0892</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304999.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0892"/>
    <dbReference type="EnsemblBacteria" id="AAZ68923">
        <property type="protein sequence ID" value="AAZ68923"/>
        <property type="gene ID" value="Ecaj_0892"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0892"/>
    <dbReference type="eggNOG" id="COG0617">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015961_2_3_5"/>
    <dbReference type="OMA" id="YNYELRI"/>
    <dbReference type="OrthoDB" id="2018439at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052928">
        <property type="term" value="F:CTP:3'-cytidine-tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CTP:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016437">
        <property type="term" value="F:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PolyA_pol" evidence="4">
        <location>
            <begin position="26"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="PolyA_pol_RNAbd" evidence="5">
        <location>
            <begin position="187"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="396" mass="45598" checksum="836FF1CF9E77AAD9" modified="2005-09-27" version="1">MYDTLVSNDDILSIINAIKKFQGEIRLVGGCVRDNMLKRQTTDIDFATTLLPNQIVESLTTANIKTIPTGIKHGTITAIVNNTAYEITTLRSDINCDGRHAEVKFTNNWRQDASRRDFTFNALYCDEQGIVYDYFSGIEDLEKKQLNFIGDPEIRIKEDYLRILRAFRFYTSICSKNTLSDQIIYCCTKYSSCINKLSKERIRDEFFKLLSYANLTNTLKIMQKCQVLEKIIPFDITPDIMSSDILSNTDPLTKLAALLRTNKCSLDTIKVSLCLSNHDQKILALLLNNNLELPLSIIEQQKYINKLGKKVYCNLMKIRHAELNLNHHDLVQYIEYADKFIIPEFPISGKDLFNIGYQEGKNLGKTLKKIKDLWEDSSYQLTKSQLLNHAKELLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQT3</accession>
    <name>Q3YQT3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded-DNA-specific exonuclease RecJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0891</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304998.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0891"/>
    <dbReference type="EnsemblBacteria" id="AAZ68922">
        <property type="protein sequence ID" value="AAZ68922"/>
        <property type="gene ID" value="Ecaj_0891"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0891"/>
    <dbReference type="eggNOG" id="COG0608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009736_5_1_5"/>
    <dbReference type="OMA" id="FIICDHH"/>
    <dbReference type="OrthoDB" id="491634at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004610">
        <property type="entry name" value="RecJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041122">
        <property type="entry name" value="RecJ_OB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17768">
        <property type="entry name" value="RecJ_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00644">
        <property type="entry name" value="recJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="7">
        <location>
            <begin position="93"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="8">
        <location>
            <begin position="341"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="RecJ_OB" evidence="9">
        <location>
            <begin position="472"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="330"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="584" mass="64412" checksum="9A01CE657DBCB7CD" modified="2005-09-27" version="1">MLHCNLENSEYIGVTGALWKPHNVNLRDILTIKQKFCLSEIAARVLSTRKISIEEIHDFLYPTLKASLPDPFHLLDMDKAVNRICEAIYNKEKIVVFGDYDVDGATSSALIKQYLTQIGVPTTIYIPDRICEGYGPNTQALLKLKEIGNSLCITVDCGTIAHEPISAAKSVNLDVIVIDHHIGMNTLPDAIAVINPNRLDENSPYTYLAGVGVSFLMLIALNKTLKEKGFFTNNTEPNLINYLDLVALGTVCDVMPIIGLNRAFVKQGLKIIATRQNIGLKVLSDVIGLEEKPNVYQLGFSIGPHINAGGRVGNASLGARLLSSNNEEEALEISEKLQNFNLERKELEHQSFNEAVEQAETLMSTNTNLIIVAGNWHPGIIGIVAGRLKDKFFLPSIVISLNNGIGKASARSIPDVDLGAAILSAKLEGIITEGGGHAMAAGFSINEDKIKTLYEFLSQRFHNINKQKVFKVDGVITASAINLTLWKELQFLEPFGIGNPEPKFILTNIKIKNPEIIGDSHIRCLIYDNKTFIKGICFRCVDTELGLALLERTAVMLLGKISVNYWRGNENIQFIIEDAITKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQT5</accession>
    <name>Q3YQT5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Signal recognition particle-docking protein FtsY</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0889</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304996.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0889"/>
    <dbReference type="EnsemblBacteria" id="AAZ68920">
        <property type="protein sequence ID" value="AAZ68920"/>
        <property type="gene ID" value="Ecaj_0889"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0889"/>
    <dbReference type="eggNOG" id="COG0552">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_3_4_5"/>
    <dbReference type="OMA" id="GISDQFQ"/>
    <dbReference type="OrthoDB" id="656414at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF7">
        <property type="entry name" value="PTHR43134:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54_N" evidence="8">
        <location>
            <begin position="13"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="104"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="SRP54" evidence="7">
        <location>
            <begin position="105"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="310" mass="33985" checksum="5AE991F106171CD2" modified="2005-09-27" version="1">MADTNNKGFLNNIKKGLFKTSSKLSDGIKKIFSSEKKIDQETLEELKELLITADVGYENASLLTQKLAEIKFNEVDYSIVKQKLAESIEDILLQVEKPLSINNKPHVIMICGTNGNGKTTTIGKLAHKFKNEGKKVLVAACDTFRAAATEQLIVWSQKVNFPVVTGNQGADAASIAYQAMQQALNDQVDVLLIDTAGRLHNHKNLMEELAKIKRIIGKHDNNAPHNVILILDATTGQNAIHQVEAFSQFVNISGLIMTKLDGTAKGGVVIKIAQKYKLSIHAIGIGEHVEQLQDFSAKEFAIGLLDIENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQV5</accession>
    <name>Q3YQV5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SURF1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0869</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SURF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0869"/>
    <dbReference type="EnsemblBacteria" id="AAZ68900">
        <property type="protein sequence ID" value="AAZ68900"/>
        <property type="gene ID" value="Ecaj_0869"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0869"/>
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        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047737_4_1_5"/>
    <dbReference type="OMA" id="LEKCILW"/>
    <dbReference type="OrthoDB" id="1908280at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06662">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002994">
        <property type="entry name" value="Surf1/Shy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045214">
        <property type="entry name" value="Surf1/Surf4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427">
        <property type="entry name" value="PTHR23427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02104">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50895">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="208" mass="24796" checksum="4023E60AC0562DD0" modified="2005-09-27" version="1">MWLRLFIVFMLPFSTMVLLGTWQIFRLREKTNIIHAMQDPPSKLKSHDIIKQNYRHVSVNGVFDNNYRFFVFAKTLGYYLLQPFHLSDGRYILVNKGTVLNKEDKFELSSTNLLNIQGILSCDSNKKIGWFVKNDVDANIWFWFDIESMMKQINIPLENCIIWSDNTFDGIKPNIPLKVRNDHLEYIITWYLLALIWLVGYMYLCYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQW3</accession>
    <name>Q3YQW3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD assembly factor SdhE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0861</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SdhE FAD assembly factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304969.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0861"/>
    <dbReference type="EnsemblBacteria" id="AAZ68892">
        <property type="protein sequence ID" value="AAZ68892"/>
        <property type="gene ID" value="Ecaj_0861"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0861"/>
    <dbReference type="eggNOG" id="COG2938">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103054_1_0_5"/>
    <dbReference type="OMA" id="LFRCWHR"/>
    <dbReference type="OrthoDB" id="2055100at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="SSF109910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="93" mass="10921" checksum="70F1D241B8F3F045" modified="2005-09-27" version="1">MDELLAKRKKLLYRSLHRGCKEMDIILGNFASYYIHLLSDEDINIYEKIVDTNDHQLYKYITGEEFIPQDLDGNVMRSIIALNESLVKSRLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQY4</accession>
    <name>Q3YQY4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304948.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68871">
        <property type="protein sequence ID" value="AAZ68871"/>
        <property type="gene ID" value="Ecaj_0840"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0840"/>
    <dbReference type="HOGENOM" id="CLU_148745_1_0_5"/>
    <dbReference type="OMA" id="TIVIKVM"/>
    <dbReference type="OrthoDB" id="1910088at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007039">
        <property type="entry name" value="TrbC/VirB2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04956">
        <property type="entry name" value="TrbC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="120" mass="12579" checksum="1584BF106B11EAD5" modified="2005-09-27" version="1">MLKIIVMFFTIVGFISVTPLAFANSSSDTTGAQDEVSKVICNVILFVQKLGLPIMTGVILGSSIMAIFGKLPWPAIVMLVVFTAIFFGAGKLVSKFVAGVNNNLTSGSDCTSGSTPEKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQY9</accession>
    <name>Q3YQY9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68866">
        <property type="protein sequence ID" value="AAZ68866"/>
        <property type="gene ID" value="Ecaj_0835"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0835"/>
    <dbReference type="HOGENOM" id="CLU_278086_0_0_5"/>
    <dbReference type="OMA" id="GGHRYFW"/>
    <dbReference type="OrthoDB" id="38092at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="947"/>
            <end position="1023"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1267" mass="143916" checksum="664CC9136D6E4AE1" modified="2005-09-27" version="1">MVECVLRIIALVVFCLYFTFPDSSFGLDPDSSLDANTCSSSSVFYTLDQINKGPYPFCEGIYKEIPILDFRIVPFYLQSFFDPKLFLMHPTKGLKLVPKNKTSISTDFQTVMSNGIKSGRDDLNLSDYGVKLYGMHRSGMDSLCIYYNSRGQCVKQCLPIPALRRPVLRANDKEEIVAEFKVRSVIEGNLKSATSRDYKEKIEEMSLEGLQAIFGKSVGLILPKIDVKNHEFETIDGCTEKDENEKCIKSKKEIATSIYVDNIKCLGGLNYAENGYYVKRYDKSIGGHRYFWLRSNKKKFVRHIYSNGIYYPCDDSMSYDLDNISMKSFVTSIKGHDTHYTIPKVKYNLMPYSGDKKSNLCKNSELYFYIPTYLRRIKDEKECIFGELKYSSDEKEHTKSCLYSYTSDDFETFGAYKNKSELKNLDFFLQNDGVLSGLVERNLYFEGMCIDKFPKYEYKVKKHVDGTVTKQYVYEIGNYHKCDFIKIEAWGGGQAGYVNSGTAYSGTPAYYTLGILKNDNNKLNGKKLVIYVGEGGKYPREYGEDTVVALCNSDNFDNSCQISLVARGCTHNKCRDNSSFINDDTVVHYRSATGMDFNQQRPLWLQHYRLIPWGNSNFPEGIVRLNVNDCNGPLNAFEKSPNQYPGSGGCAKLGKSIQQGADGLVRLTCEMWSSGSKVQIQDMSYTYNKSKKSLCSVNSDSGCLKSICIYSSNDETMKFSTPITMFGEEECKPIVSNIPNLFVNLSKAGDKMTVSRAHNGYCYNNHKKDLRLIYGSNVDNALYENNLRINYKKCWQDNEHHDIGLSCYRNRASDLKVPNDKYICCYYDMKDTSHHKDICWKTNGADLLKKFSYSTLPLALREKFDNGSKELLTKLPPSNTIKPFADRPDVITDIKYDYSKEKTSFCLNTKDGIKKGCLQTVCVYEKGENDVFASGTYLITRKGVQCPSKNEMRVEDSEISENINLSCKSDGKCCYSSDNKKAEKLCWANEEVSLLCYEDDDTKDSVKDNIKGGKSESDGVYKCCYGKLEDYKSPSKNDKKRICWKKSLTDLTNMLVGDDAVELAKLNTKEIKGKTQYRSSQVKMIEGKLVELEYIYDQEKKTFCLDTTDTLQNRGCLASLCIYAKDGTLNSQSSSYPTSLCITQSAMNTGRSATKMGKIGQSVKMSCTNEHCCYGNGNGERMCMTDEKLVLSCYETGNISEKGEFDYTCCYTNTDKTSENVCWKVSDTIWQILGYYRLSEKVREQINEHYNKPTIRVVKGESGKPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQZ0</accession>
    <name>Q3YQZ0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68865">
        <property type="protein sequence ID" value="AAZ68865"/>
        <property type="gene ID" value="Ecaj_0834"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0834"/>
    <dbReference type="HOGENOM" id="CLU_372898_0_0_5"/>
    <dbReference type="OMA" id="QCADYEY"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="782" mass="90480" checksum="3DAE26883E4D186D" modified="2005-09-27" version="1">MMFRHVFNIVKKVGYVFILILYTGNVYSLEDRQVFNVKPSFSGYRYLKFLNDEVIIDNAMFCTIIKAGECRTAGANRLPHIAYFDTSNFAYHKLCVCGVSSCEDLPDEQSEWSARCETTAVCVKTVQKLLPLQFCTVLFDEKSSMRFVPIEFSRQMFFNPGIRLIVVDNGKLYKQDFFLGVGVMSRNCDVTFAEKLYKFSIYKVGNSRICASYYDQGNFLTKCVPIPALPKPILHGEEDGKIKMSFNGKLIVTQDKRVSDEIVDHFGISIVRPKINFDNHQFYLYQKCKDGKVLKLDEYCQDNAVGQIRYQHDDKVTVKCIDGINTTFGYVLKSHNVYDKHTWLKPLPNKMVRYVLTDKKQYIQCADYEYDLTNKNQDFLDSILINEDNYYFFSDEQHRNKKLNLYQDDNPCNNLKNSFYLYQNTKLKIADNKSTTLPPEVFTQFIKSDSLNSNVEYFNLKNQKILSADPMIKSYRFDTHVLRYFNVDNKEMLSLDPKLQLELQPLDYHSMGMCIDSFESTTYVPEKMTESDKNFDSALPVLQEDNKSESVLLHYPKLSGIYNVPGKCDFIKVEMLGGGESGKIDIKSWIGKEGQPGEYVMGMFKTKKSDKYFVKIYFKRMENKSSKSENDKHLKDNSDVGMDAVIEFCKKDERLHDGEVCEVQLTANGGGKLQHDIRKNVKSNNLVWDKRMLYYRVVNSHVSRRSENVPHDKRIRFIPYQNFITEALWEEIEKDECKSGKLLAENNSKYFGAGGCVDVSTGSTERGASGMVKITCEDWYHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR03</accession>
    <name>Q3YR03_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 7">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpsI</name>
        <name evidence="8" type="ordered locus">Ecaj_0821</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304929.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0821"/>
    <dbReference type="EnsemblBacteria" id="AAZ68852">
        <property type="protein sequence ID" value="AAZ68852"/>
        <property type="gene ID" value="Ecaj_0821"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0821"/>
    <dbReference type="eggNOG" id="COG0103">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_2_1_5"/>
    <dbReference type="OMA" id="QWKINGR"/>
    <dbReference type="OrthoDB" id="1766414at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_B">
        <property type="entry name" value="Ribosomal_S9_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_S9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_S9_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="149" mass="16357" checksum="3A7675071813F199" modified="2005-09-27" version="1">MESNPDMLSDHLQGNRLKVDAYGRAYGTGRRKEAVARVWLTVGKGNVTVNGLDIFNYFKKNTLCRKVYHPFIATSTSGCYNVVATVRGGGISGQAGAVAHGISKALKEINPSLHAVLRAGGFLTRDSRVVERKKYGQHKARKSCQYSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR49</accession>
    <name>Q3YR49_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Insulinase-like:Peptidase M16, C-terminal</fullName>
            <ecNumber evidence="6">3.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304883.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0775"/>
    <dbReference type="EnsemblBacteria" id="AAZ68806">
        <property type="protein sequence ID" value="AAZ68806"/>
        <property type="gene ID" value="Ecaj_0775"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0775"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_3_0_5"/>
    <dbReference type="OMA" id="IDVVCDM"/>
    <dbReference type="OrthoDB" id="1188251at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16" evidence="4">
        <location>
            <begin position="16"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="5">
        <location>
            <begin position="166"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="421" mass="47742" checksum="567540D00CD977D6" modified="2005-09-27" version="1">MSPSITQFRNNFTIITDTMPHVESISINIWINVGSRYENTNITGISHFLEHMAFKGTKTRTALDIAQIFDDIGGNFNAHTDREHTVYHVKTLKRDIKIAIEVLADIILNSQFPQEEIDREKGVVLQEIYQTNDSPTSIIFDKYIEAAYPNQIFGKSILGTPESVTNLSKEDLQTYMSEYYHAGNMLLSVAGNITHEEVIDLVSQHFSNMKKSEPKTAAPSVYYSGEYREIRNLEQVHLVIGFPSVSYKDDLFYTIQILDSILGNGMSSRLFQKIREQLGLVYTISSFNSSYSDNGIFSIYAATDKNNLIQLLTTIASEVKSITMNLEENEITRAKGKLISEILMSRESTTARAESLGYYYSHYNRYILKEELIKKISEITLTDLQNCIHNLLGSNNKITLAAIGQIENLPSYGDIVQMFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR63</accession>
    <name>Q3YR63_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304869.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0760"/>
    <dbReference type="EnsemblBacteria" id="AAZ68792">
        <property type="protein sequence ID" value="AAZ68792"/>
        <property type="gene ID" value="Ecaj_0760"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0760"/>
    <dbReference type="eggNOG" id="COG3164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_300490_0_0_5"/>
    <dbReference type="OMA" id="GHLNEPL"/>
    <dbReference type="OrthoDB" id="49944at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032712">
        <property type="entry name" value="AsmA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025263">
        <property type="entry name" value="DUF3971"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13502">
        <property type="entry name" value="AsmA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13116">
        <property type="entry name" value="DUF3971"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3971" evidence="2">
        <location>
            <begin position="340"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="AsmA_2" evidence="3">
        <location>
            <begin position="778"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="996" mass="112629" checksum="DC1AC80C24179176" modified="2005-09-27" version="1">MLKKKKFIKLFCILIISIGCSLSYLYIRDKDIIDINAKFLNFYIKHKLATVFPDSEINIGKTTLTWQNKSENFILSSQDLTIKNAKFGADITIPEFLLYSRVGILFLWGNCDFSYIDIPKVRIEFYDIEKKVEVNLIENFIKAVKDILLNAIKLDIPVFISNAVLAKSGKEDLIIKQLNMKISKGYDKNTIDFNLESDNSFVSMTVHEHYKGIMSLEMSYGNFSTNILGYLGNLNTQLPLYDNLNFSGDVVLKIDEYDKITYGDVNVQSIKGVIPCVSSRKCYVHDFRTRLVYQNDILSVKNFFVLLDQSKIVANGVIDDESVNLNFNIDVVSPEVLCNYWVANLYPDLNHWYCTNVKEGKISDLKLQIKGKWDNIFLYNDLSSYNVNADIKNVLIKFNDNFDPVRIVHGKLYLYDNKFIITSDNSDFKGMVVKDGILAIEDLRDTNAVMNISGNSVSNVKQLYLAVDDKEFIPLDDKKIFGNAFTTFNFKIFNLLNDDVIDYESDIHTLVESFKADSILSAFDVYDTKVDIILHDNDVNIDAQGHMNGYPMSLKISKNLQNNYKSHYAFTGYISTENVKRLGVFDYENCSGIMRSNLQWDTDGDNTDITGDIDLAHLKIYFNGLAKKNFNSVVKFFASFKDKNEVKIGNASIVGDNIDIELSGRVGNNIELLLNKVKLENTDVKAELKKNEDSIAVKVFGKSLDLSAFDFTDVMKGRSVIKQANVQVNVDRVIMKNDVITKNIDFKLERDGGVYRIIKLTGDFVDSGNFNMEYGPIGLEISTNNAGELLRALNILDVINKGKLSFYMYPINTNDVTHGMFSLTNFHLVNASILAQILTLSSLKGVVNTLNGKGIYFNTLNVPFTYQDNLVNIDESWMEGSELGISLGGELNVDTNMFDIKGQIIPAYVINKIIWQTPIIGKLLTGGQSRGVIAIDYKVKGTDKDHDLSVNLMSILTPSLLKRVLQVFDTKLTKKDNVKNKSSVQKVKENRVSKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR69</accession>
    <name>Q3YR69_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304863.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68786">
        <property type="protein sequence ID" value="AAZ68786"/>
        <property type="gene ID" value="Ecaj_0754"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0754"/>
    <dbReference type="HOGENOM" id="CLU_1033408_0_0_5"/>
    <dbReference type="OrthoDB" id="2172620at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="269" mass="30313" checksum="362E47D23D0BBBA3" modified="2005-09-27" version="1">MSNITTHNESTIALFLLLILLLFISIFGLAVYISMSTNASTQIGLLCGASAIMLILVVATVYYLRLGGEVSAACKSVMQEVEEKLDGQLIGCDSKGVRALLGASLAIDYIVSEFENFKKICSDSDEKRDRIVNMERTLSDGKMLAYMAGIYQTRELSKDEQQVLVRLKRSHYHRRQDSLRQLSLDMVGRCLREIISMLEVPRFTLKDCFKQEKISLGRIMVAYNIFYDHMSCIGEMVRSKSLKMVIDESAQSVKFDVVESVVPSLRYSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR73</accession>
    <name>3MGH_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative 3-methyladenine DNA glycosylase</fullName>
            <ecNumber evidence="1">3.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Ecaj_0750</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA glycosylase MPG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304859.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YR73"/>
    <dbReference type="SMR" id="Q3YR73"/>
    <dbReference type="STRING" id="269484.Ecaj_0750"/>
    <dbReference type="EnsemblBacteria" id="AAZ68782">
        <property type="protein sequence ID" value="AAZ68782"/>
        <property type="gene ID" value="Ecaj_0750"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0750"/>
    <dbReference type="eggNOG" id="COG2094">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060471_4_1_5"/>
    <dbReference type="OMA" id="LPWRWYL"/>
    <dbReference type="OrthoDB" id="1385409at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003905">
        <property type="term" value="F:alkylbase DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00540">
        <property type="entry name" value="AAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00527">
        <property type="entry name" value="3MGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003180">
        <property type="entry name" value="MPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036995">
        <property type="entry name" value="MPG_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429">
        <property type="entry name" value="PTHR10429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02245">
        <property type="entry name" value="Pur_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00567">
        <property type="entry name" value="3mg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000265016" description="Putative 3-methyladenine DNA glycosylase">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00527"/>
        </source>
    </evidence>
    <sequence length="189" mass="21645" checksum="26A680FE80F46763" modified="2005-09-27" version="1">MQYNILKKSFYAQQSLDVAEKLLGKKLLFNKHQGIITETEAYIGHDDPAAHSSHGYTKRTSVMFGDPGFSYVYFIYGMYHCLNVVTEPRGFPAAVLIRGIVLLLEDQPNIIINGPGKLCKILHITKEHNNIDMTQNYNFCICNTGINIHNYICTPRIGISRGKEKFWRFVVSDLTFLQHLKLENKVYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR74</accession>
    <name>Q3YR74_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304858.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68781">
        <property type="protein sequence ID" value="AAZ68781"/>
        <property type="gene ID" value="Ecaj_0749"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0749"/>
    <dbReference type="HOGENOM" id="CLU_1803099_0_0_5"/>
    <dbReference type="OMA" id="KQLLYWH"/>
    <dbReference type="OrthoDB" id="1757945at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="143" mass="17019" checksum="CE2B701826043C66" modified="2005-09-27" version="1">MKSNEKTDTGDKKVDKLKKFENTVSKVAQFIIGDSLSIQLKEYKVSFSYIIDNTKDYLKSVNVEEIKIIEKVNQLKTINNTLLERLFNFWLRKNKQLLYWHMMFLLINVILLIINGDQIVRLSNLIFEMFMTWKDKILSLYCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR86</accession>
    <name>Q3YR86_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0737</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304846.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68769">
        <property type="protein sequence ID" value="AAZ68769"/>
        <property type="gene ID" value="Ecaj_0737"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0737"/>
    <dbReference type="HOGENOM" id="CLU_1479843_0_0_5"/>
    <dbReference type="OrthoDB" id="2171186at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="182" mass="20321" checksum="53ECDAF23034BE25" modified="2005-09-27" version="1">MGNLDIQKYVYLALFLLLLVILLMLAIYRYCVASEHDLQRQDVLVLSREYYNQLAGGYRQLKNDQSVMFVHNVRLAKTCEAVAEEKRNLEKKIKELGDDMSEVSVSMAVELNSLGVHPTVVSSMTSQMLSLQRRIYDVARSSPVSIVKADQGVQTEQQQASSALDQILASGLQCNTGGKVVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR88</accession>
    <name>Q3YR88_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68767">
        <property type="protein sequence ID" value="AAZ68767"/>
        <property type="gene ID" value="Ecaj_0735"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0735"/>
    <dbReference type="HOGENOM" id="CLU_2301388_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="100" mass="11364" checksum="2EDEAA7C30903288" modified="2005-09-27" version="1">MCVMDKVKIALLLTVVSLLLLVIVIFSCLKIYRGNKSYQKLLNQCNELHSKYDDLVRSHEVIVSQLKGELAFLREGCSALEMKNEALRSSDCDTKELEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR93</accession>
    <name>Q3YR93_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68762">
        <property type="protein sequence ID" value="AAZ68762"/>
        <property type="gene ID" value="Ecaj_0730"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0730"/>
    <dbReference type="HOGENOM" id="CLU_1608273_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230973" evidence="2">
        <location>
            <begin position="18"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="165" mass="18485" checksum="64B400DEF3B32240" modified="2005-09-27" version="1">MCIVMLMLLLLIAMCQCCISMRTLDGKFESEAMAEQRIQELVDENQNLVLENRELSAACKSANGKLSELLLQMKNLLLSAETQSEKILNDLALDNQANLCTKKAVQCGVLKIRQGIFDIVHNNREEQRRNIELGGNTPSKIPVSKLRMQSLSTLQSENKVQSCCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRA8</accession>
    <name>Q3YRA8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304824.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0715"/>
    <dbReference type="EnsemblBacteria" id="AAZ68747">
        <property type="protein sequence ID" value="AAZ68747"/>
        <property type="gene ID" value="Ecaj_0715"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0715"/>
    <dbReference type="HOGENOM" id="CLU_235426_0_0_5"/>
    <dbReference type="OrthoDB" id="2104784at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1222"/>
            <end position="1241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1918" mass="210317" checksum="583A77F201BA4E41" modified="2005-09-27" version="1">MKDCYKIALLVTTILVLIFLILLMASIFLIMIYKHNKKVMQTIQHPTIDSTDLRVEEASDDEEYDPMDRDLLYCDEEGENVRGVLDAQTTTDEGSNDVPPSTHVNQETSHTARVEQIDKATQTQETLNAQEEVQVNKHTNDVGNDTNESLAPIVNISREGLAGYSRLLEEVVDDKLNLTTQNPTFAGVGSSLSNTHDVTQKAYITERETEREKVETEVKVQETLSVKKTEYQGASSKFFNDIGSNVSKDLPPFVNTTFCREALNGELSDIEMDDDSDSCLLYSGMKKKVPYFNADFYAGNGYENASGAYYSYHVSPDSLSTTASARGKYDGVFAQAIEEGLERISSGLQNKSIGAAQGDSIKDCAGDDISPVVRIAGNECLVGNDDLVKETTDDDDNSKLVSLNAPAADSNRSGISVLSDVNPVANYTAEVGKEYVAKNGETNVSQSGGVIFPNVKEHVSFNSEQSGNGKKRVSFSLHPTIFYYSTVFDEVSRGNDGVSEESREKYAVKYEQEDSTTFSLSTSQEHLKDETSIATGQDIVDCDKTTENYFVSLPNDVSTVQGAANDSETNIHKQTTLIDVVERKSLHLCDTVSLGSGSSKTMVVDSTATASACGLVEKSSIENATATSTSAVSTPEMRQELRTSVVESSNVSQKFVEPVAFDSGKESSSSAVPAEFSSESIGTATVKGELPNEAVDQSMSTSLNQALVPYAVGAVVDTAGNSFDTNMFRINEQLSDVLPSEKTTLVITYGVDGGSKNTFVCQVNHNSITSRFNLSVNEIGMLNSRNTDSGVDFVERFLQPRFPYGLQQRFSLDQLSMLYPRHANPDIIGSRAGFLQPVPYRLQQRFSFDQLTMLYPRHANPDIIGSRAGFLQPVPYGLQQRFSFDQLSMLYPCHTNPGMIGFRARFLQRGLSQQLHELQKTAVQVCNFDESITTSVKPVAAVNSISEMQRELPIVTDSHLASSSLSQEFMESETTVCSKEGFQDRSAKPIATINSVSEMQRELPIVTDSHLASSSLSQEFMESETAVCSKEGFQDRSAKPIAAINSVSEMHRELPIVTDSHLASSSLSQEFMESETTVCSKEGFQDRSAKPIAAINSAYEMQSKLPIMTGNHLASSNGRQELILSVAAFDLAKESSSGYTEENPAAIVSTSEIRESTETIHTAGSMRQGLVLSTTGDLAKEFSSGYTEENPAAIVSTSEIRESTETIHTAGSMRQGLVLSTTGDLGKESSSGYTEENPAAIVSTSEIQESTETIHTAGSMRQGLVLSTTGDLAKESSSEYTEENPAAIVSTSEIQESTETIHTAGSMRQGLVLSTTEFGIAKVPDSIDTSKNNGRLSSVLQDDDNNIFGTVSFVTNEIKSVCVNKVKSNISFISNGFDIAVRKGKMEFKMLYPHAANPNIGDRAKFLQPRLSIELQQQLSKLQKTAVQAYAVDQGIENLLSTSTVESIDTTEMNGQLFDEPIYHLVDSDVNQKLIKPTSPAVFDLEEESFMENASAKPTTAIIDMHEIQKKLPTGTDHHLASSSVSKELMGSTISGVFNSAEISNGQTCEAVLTEPVVERVTNTTKDDMQYDDLPLIDNRYELVVYNLIEERKAKGNVTVKSSSMIIEQEILLVDCYDIIRLSMKDLKMDFVDISSVELEDVSLLFLERGQLVTKKLLLSIIKNISPIFVRVFSGGDVSDNEWKLLEMLLNDRRICENFYVKRILCYYAVIIEYAGVLNKEYSKLLDRKLKIGGKIERKRAEIDRAIVNDGKPHGKFSLSKFKPSVATLKKELSELNSNFGSNAILLSECEEKIKQLKNERVAFVNNDSDYMNSLVSCIIGDFDIYDDRANNVVKNCYTIGNFISKDIGEDLEMLVLVLKCSVVDSSGFVKNIILRMKQINSKKAALLNTEQTRGMLLGVATTNGYEVVSACGSVRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRF3</accession>
    <name>Q3YRF3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutathione synthetase</fullName>
            <ecNumber evidence="10">6.3.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GSH synthetase</fullName>
            <shortName evidence="10">GSH-S</shortName>
            <shortName evidence="10">GSHase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glutathione synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gshB</name>
        <name evidence="13" type="ordered locus">Ecaj_0670</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + glutathione + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the prokaryotic GSH synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304779.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0670"/>
    <dbReference type="EnsemblBacteria" id="AAZ68702">
        <property type="protein sequence ID" value="AAZ68702"/>
        <property type="gene ID" value="Ecaj_0670"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0670"/>
    <dbReference type="eggNOG" id="COG0189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068239_0_0_5"/>
    <dbReference type="OMA" id="WMRKDPP"/>
    <dbReference type="OrthoDB" id="878336at2"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00162">
        <property type="entry name" value="GSH_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006284">
        <property type="entry name" value="Glut_synth_pro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004218">
        <property type="entry name" value="GSHS_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004215">
        <property type="entry name" value="GSHS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF4">
        <property type="entry name" value="PTHR21621:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02955">
        <property type="entry name" value="GSH-S_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02951">
        <property type="entry name" value="GSH-S_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01380">
        <property type="entry name" value="glut_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="3 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="118"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="313" mass="35705" checksum="DE7049B020E457F6" modified="2005-09-27" version="1">MALVVAFQMDRDVVVDKDLSVKFIQEAQARGHTVFFYSPDQLSIKGNVPVARACLVTLDGGRLKFANVQELLLEHVDIIFIRQNPPFDMRYITCTYILEKLKKPLLINHPRSLRDYSEKLLPLLFPEFIPDTVITEDRLILQDFYQEHRDIVLKPLYSYAGNDVTRMHSNVDINVVIELMIEKYGCPIIAQKFIPNISNDKRIVLLDGKPIGVLKRKISFDGEIRTNLSIGSIPEATALSDRDLAICSAIEHELLGKGIIFVGIDVLDRYLIEVNVTSPCGILQINELYGVSLEKKCWDCFEEKFYKKLNGCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRF4</accession>
    <name>Q3YRF4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cell division protein FtsQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0669</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304778.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0669"/>
    <dbReference type="EnsemblBacteria" id="AAZ68701">
        <property type="protein sequence ID" value="AAZ68701"/>
        <property type="gene ID" value="Ecaj_0669"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0669"/>
    <dbReference type="eggNOG" id="COG1589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061141_3_0_5"/>
    <dbReference type="OMA" id="WSIIDMR"/>
    <dbReference type="OrthoDB" id="1270665at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090529">
        <property type="term" value="P:cell septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005548">
        <property type="entry name" value="Cell_div_FtsQ/DivIB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026579">
        <property type="entry name" value="FtsQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045335">
        <property type="entry name" value="FtsQ_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013685">
        <property type="entry name" value="POTRA_FtsQ_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35851">
        <property type="entry name" value="PTHR35851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03799">
        <property type="entry name" value="FtsQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08478">
        <property type="entry name" value="POTRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="9">
        <location>
            <begin position="84"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="275" mass="31532" checksum="D0A93B5BE03E9618" modified="2005-09-27" version="1">MREISDLKDIDDYRSLSNAGNIGSVNIFKSSMRFFTRKNLVFFLMIIISITFIYFFKGEIANKFRNCALIISHYISDKLINCGFAVDDIVINGNKFVPSDYIRGFVSVNKSILFLPLSELQKEIKDSSKWIKSVSVKRLLPNVLQIRVLEYLPFANWYHDDGSSIIDDTGHVIVSDYDEQDDLVSIYGNEALQGLHFIKKLVNENSVLSNMISSMFYFDDGSWDIVLSSGLNIKLPKENPYNAWNNLLSICEASSEFLIWKTVDMRVPTQINIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRG0</accession>
    <name>Q3YRG0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GGDEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304772.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68695">
        <property type="protein sequence ID" value="AAZ68695"/>
        <property type="gene ID" value="Ecaj_0663"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0663"/>
    <dbReference type="eggNOG" id="ENOG5032BP7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_942449_0_0_5"/>
    <dbReference type="OMA" id="GYAQMYN"/>
    <dbReference type="OrthoDB" id="1517389at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="173"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="293" mass="33146" checksum="8AB3800354D0BB23" modified="2005-09-27" version="1">MNQFVVARRADDSVISVQQNNTLQNLMISNLNESAETLLEYKAAELTNKPLSTILHKNVTENINNYLEYSSDGTDLFDILSKTINCSLIGKNNKVIPIKPKVFRTTAYNQDIINYEILVRDASMSQKLDIFRQSVLPNTKHNMHPFFNIMDEASTNKEIKIVLDFLHKYNIHAVIGIIQIDPPHNSSNIDTLTQYTINLLHKNVRESDIVGYIGDHKIICLLLGCKSEYAHTAISRIHKNINNNLQNLHVKASIGYAQMYNETDTTQILTNLNNMLFIAQQEAGGGTIKSTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRG8</accession>
    <name>Q3YRG8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase M23B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304764.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0655"/>
    <dbReference type="EnsemblBacteria" id="AAZ68687">
        <property type="protein sequence ID" value="AAZ68687"/>
        <property type="gene ID" value="Ecaj_0655"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0655"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1388344_0_0_5"/>
    <dbReference type="OMA" id="CFTIRHN"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M23" evidence="1">
        <location>
            <begin position="99"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="201" mass="22671" checksum="8975C6A7FCBE791C" modified="2005-09-27" version="1">MTIRKTLLIALLCFGCTQKPAPYFLRGEEFHGKKEIDDINEYHLVKYNVQENTESNTTQDTSNVSKETKIFDKNCNFIMPIEGIITSKFSINNQDKNCKEGINIKSNGFQHVKASSGGKILYVGKGSKWYGNMIIIEHNKNTITTYSYLDTINVKIGDKVTQGQVIASIKSTNPQTNKSHLCFALRKHGKAVNPLLYINCK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRH0</accession>
    <name>MNMG_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glucose-inhibited division protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmG</name>
        <name evidence="1" type="synonym">gidA</name>
        <name type="ordered locus">Ecaj_0653</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MnmG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304762.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRH0"/>
    <dbReference type="SMR" id="Q3YRH0"/>
    <dbReference type="STRING" id="269484.Ecaj_0653"/>
    <dbReference type="EnsemblBacteria" id="AAZ68685">
        <property type="protein sequence ID" value="AAZ68685"/>
        <property type="gene ID" value="Ecaj_0653"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0653"/>
    <dbReference type="eggNOG" id="COG0445">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007831_2_2_5"/>
    <dbReference type="OMA" id="FRPGYAI"/>
    <dbReference type="OrthoDB" id="146811at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="PTHR11806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PTHR11806:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00136">
        <property type="entry name" value="gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01281">
        <property type="entry name" value="GIDA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000016595" description="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG">
        <location>
            <begin position="1"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="273"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00129"/>
        </source>
    </evidence>
    <sequence length="625" mass="69038" checksum="A285C2EB09294FA3" modified="2005-09-27" version="1">MSHYDVVVIGGGHAGCEAAAAASRIGAKTLLITHSISTIGEMSCNPAIGGIAKGTVVREVDALDGLMGKVIDKASIHSSILNRSKGPAVWGPRAQADRLIYKQTMQEIILNYPNLTVLEASVEDFTITQDNQKDSVKAVITSEQHTIYTSKLILTTGTFLQGTIHIGSYTTPAGRIGEQPSIGLAKTLEKYNFKLGRLRTGTPPRLDINSINFAVLQEQKGDIHPSPFSYISQSIDLPQISCYLTATNNKTHEVIKNNLHRAAASNLLKDIKAPRYCPSIEEKVRRFSDRNSHQVFLEPEGLNSDIIYPNGITTSSPLDVQYEMLKTIPGLEKVNIVRSGYSVEYNFIDPRELYHTLETKKISGLYFAGQINGTTGYEEAAGQGIIAGINAALSLSSNYEPFILKRSDAYIGVMIDDLVTLGTSEPYRLFTSRAEYRLRLRSDNADLRLTELGYKVSAVSSKRYCALKNKQHDIESLTNILKNIIITPTQLAAYDIPVSQDGVKRSIFDLLSHPNINIETVSKICDVIKKFSKAVVSQVEIEGKYAPYFIRQDADIQDFIEEENTHIPSNIEFSQIHGLSKEIQEKLAHIKPPSIGSARRIPGVTPAAITSILIYLRYHKNKKIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRI1</accession>
    <name>LIPB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Octanoyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.181</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lipoate-protein ligase B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoyl/octanoyl transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lipB</name>
        <name type="ordered locus">Ecaj_0640</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-lysyl-[protein] + octanoyl-[ACP] = H(+) + holo-[ACP] + N(6)-octanoyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="EC" id="2.3.1.181"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LipB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304751.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRI1"/>
    <dbReference type="SMR" id="Q3YRI1"/>
    <dbReference type="STRING" id="269484.Ecaj_0640"/>
    <dbReference type="EnsemblBacteria" id="AAZ68674">
        <property type="protein sequence ID" value="AAZ68674"/>
        <property type="gene ID" value="Ecaj_0640"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0640"/>
    <dbReference type="eggNOG" id="COG0321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035168_3_0_5"/>
    <dbReference type="OMA" id="GEVTYHC"/>
    <dbReference type="OrthoDB" id="1806796at2"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16444">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00013">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020605">
        <property type="entry name" value="Octanoyltransferase_CS"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016262">
        <property type="entry name" value="LPLase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01313">
        <property type="entry name" value="LIPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000242718" description="Octanoyltransferase">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="BPL/LPL catalytic" evidence="2">
        <location>
            <begin position="34"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="1">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="73"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="145"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="158"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="1">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01067"/>
        </source>
    </evidence>
    <sequence length="214" mass="24450" checksum="927ED9B6EA6C2810" modified="2005-09-27" version="1">MSCDLVEWKIESSPIDYQEAVYFMQRKVDSISDGLQKELVWLLEHPALYTAGTGATVEDLLASNLLPVYSTNRGGKYTYHGPGQRIAYVMLNLKTRNKCNVRLYVKTLGDWIINTLSHFSIKSYFNPDLIGVWVTHNGIEKKIAAFGIRIRKWITYHGVSVNIYTDLSHYLGIVPCGIKEYGVTSFKQLGVNVSYEEFDIVLEKKFNEIFSSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRI9</accession>
    <name>Q3YRI9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0632"/>
    <dbReference type="EnsemblBacteria" id="AAZ68666">
        <property type="protein sequence ID" value="AAZ68666"/>
        <property type="gene ID" value="Ecaj_0632"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0632"/>
    <dbReference type="HOGENOM" id="CLU_2878707_0_0_5"/>
    <dbReference type="OMA" id="KIMIIRV"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="75" mass="8476" checksum="ACE7E25305B76860" modified="2005-09-27" version="1">MIYCSVPLCCTKMSSNTTSLIVILVFVSLTLISLVSGVVLMAISNEFYIRNRNKIMIIRVLLQFTSLVLIALFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRJ1</accession>
    <name>Q3YRJ1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc-containing alcohol dehydrogenase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304741.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0630"/>
    <dbReference type="EnsemblBacteria" id="AAZ68664">
        <property type="protein sequence ID" value="AAZ68664"/>
        <property type="gene ID" value="Ecaj_0630"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0630"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_1_5"/>
    <dbReference type="OMA" id="THIATRE"/>
    <dbReference type="OrthoDB" id="1225778at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="11"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="324" mass="35660" checksum="F20D1D19646F0AAB" modified="2005-09-27" version="1">MVKIIVIDKTGGTEVFKYVDHNIGEPKDDEVLIQHTVIGLNRYDLECRNGMRKTRKLPTVLGIEAVGVVERLGKDVEVFNVGDRVGYCTVSGGAYSEKRIVNQKYLVRIPDDVTDQVAAAVLFKGMTAHYLTHIVYAIRPKTFALVHGATGGVGQILCQWASYKGCKVIGVISSDDRANIALRSGCSYIVNCNSKNFVEQVMEITQGTGVNVVYDSVGALTSKESFASLGMFGIYVSYGQISGAVSNVSASMLSSRSLFFTAPSVFHYKRSSFDLAMTSMELFEVIRRNHIKVSINKIYKFDDIVTAHQDLENRKLTGSNIIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRJ5</accession>
    <name>Q3YRJ5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Band 7 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304737.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0626"/>
    <dbReference type="EnsemblBacteria" id="AAZ68660">
        <property type="protein sequence ID" value="AAZ68660"/>
        <property type="gene ID" value="Ecaj_0626"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0626"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053998_0_0_5"/>
    <dbReference type="OMA" id="NLMVVLC"/>
    <dbReference type="OrthoDB" id="1213601at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="2">
        <location>
            <begin position="58"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="285" mass="31947" checksum="BB4C8BA51AC34649" modified="2005-09-27" version="1">MNSKAFHDKKLAYINGFSVILCGIVFICLLLSGIYYGNFYIVLPMSLVLLICAFIIPNGFFVNNPNEAKVVEFFGNYIGTIFEPGFFWTVPFVRMRSISLKVRNVSTSKIKVNDFNGNPIEIAAVVVWKVVSPAKACLNVGDYQEFINIQSETAVRELAGSYPYDAEDNSESLRNNSAQISSKLCDMLQNRLGIVGIVIEDARISHLAYSSEIAQIMLRRQQAKAITNARGYIVRNAIIMVDEILQHFESQYQIKLSDEQKVKLVNNLLVSLISEQGAQPTINIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRL6</accession>
    <name>RL16_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplP</name>
        <name type="ordered locus">Ecaj_0605</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304717.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRL6"/>
    <dbReference type="SMR" id="Q3YRL6"/>
    <dbReference type="STRING" id="269484.Ecaj_0605"/>
    <dbReference type="EnsemblBacteria" id="AAZ68639">
        <property type="protein sequence ID" value="AAZ68639"/>
        <property type="gene ID" value="Ecaj_0605"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0605"/>
    <dbReference type="eggNOG" id="COG0197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078858_2_1_5"/>
    <dbReference type="OMA" id="KGAVEYW"/>
    <dbReference type="OrthoDB" id="1786618at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01342">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_L10e/L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_L10e/L16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_L16_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="PTHR12220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="SSF54686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00701">
        <property type="entry name" value="RIBOSOMAL_L16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000062096" description="50S ribosomal protein L16">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01342"/>
        </source>
    </evidence>
    <sequence length="136" mass="15218" checksum="6130546F7ACBA576" modified="2005-09-27" version="1">MFIPKKTKYKKDFKGRISGNAKGGYTLSFGSYGLKALEPCRLTSKQIESARRSISRTLKRVGKVWIRAFCHTSVSKKPMDVRMGKGKGSVEMWVCKVKPGKILFEISGVSLNLAREALNKAQAKLPMKCKFVSDEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-10" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRN1</accession>
    <name>RS11_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsK</name>
        <name type="ordered locus">Ecaj_0590</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Interacts with proteins S7 and S18. Binds to IF-3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304702.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRN1"/>
    <dbReference type="SMR" id="Q3YRN1"/>
    <dbReference type="STRING" id="269484.Ecaj_0590"/>
    <dbReference type="EnsemblBacteria" id="AAZ68624">
        <property type="protein sequence ID" value="AAZ68624"/>
        <property type="gene ID" value="Ecaj_0590"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0590"/>
    <dbReference type="eggNOG" id="COG0100">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_5_0_5"/>
    <dbReference type="OMA" id="KWGVAHI"/>
    <dbReference type="OrthoDB" id="1713391at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019981">
        <property type="entry name" value="Ribosomal_S11_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03632">
        <property type="entry name" value="uS11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000294748" description="30S ribosomal protein S11">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <sequence length="126" mass="13411" checksum="4F9939993177B981" modified="2005-09-27" version="1">MSVVYKKKKRNVVVGVVYIHATYNNIIVTVTDQQGHSLICTSAGACSFKGSKKATPYAAQETASHAVKTVVEQNGMKTVSIKVSGPGAGREAAIRAVQACNLNVTSIKDTTKLPHNGCKLPGRRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRN5</accession>
    <name>Q3YRN5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ComEC/Rec2-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0586</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0586"/>
    <dbReference type="EnsemblBacteria" id="AAZ68620">
        <property type="protein sequence ID" value="AAZ68620"/>
        <property type="gene ID" value="Ecaj_0586"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0586"/>
    <dbReference type="eggNOG" id="COG0658">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011826_1_1_5"/>
    <dbReference type="OMA" id="HPSFQMS"/>
    <dbReference type="OrthoDB" id="1796527at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004477">
        <property type="entry name" value="ComEC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03772">
        <property type="entry name" value="Competence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00360">
        <property type="entry name" value="ComEC_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="421"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="514"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Competence" evidence="7">
        <location>
            <begin position="236"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="672" mass="76216" checksum="49C861B969C4897F" modified="2005-09-27" version="1">MSIIIKKITSLLYKSFNDNKNILIIWIPVLQGVGIIFYFSLDNEPQDDVLICITVLLFISLIFICLRKCGFLFLPIFFILLGFVSVQIKAHYFVSTPLQYQVYLKDVVATIKEISAKQSYKRIVVCDIENSKCNIKCIRLVVRTKLDSNITIGDKIKFSAVVYPPSMSVSQFSYDFSFVSYFKNISAVGFAVSDVYLFEKKNSRRITEYIEFVRFNIYKIFLRSLTEDSANILSALLIGKKEGISNDIMEAIRNTGIAHLFAISGLHLSFVAGLFFVLSRNLLVLYTRLAESYNIKKISAIIAIIISLIYLLIAGVPVSAQRAFIMVSCIFVGVLIDRSHNSFCSIAIASFIILLFTPESLLSPSFQMSFAAVLALIASRNIFFKLVVINNILQYFLSIIVSSLIAGLATLPYVVYHFNYFSIAGVFANLLAIPITTFLIMPLGLFYIILGVVNLEYYVSWMIESAVGLLIYVVQYISGMNKLVLLFHAFSSTSILIITFGFLFLCLWNGNLRFCGLILVACGFFVGVNYVTPDIMINQHNVVVKELDNKLYAINQKAHLTSFINLTWAKQNGQREMLKHPLRNNQCLSCKKGEGCIYSKFGKKVLIAYNANYVLRNCINVDLVIQGKKFVYPHECVTQHIAYNDKRRSYFVWVTKNHIKVNEISGNRIWHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRQ1</accession>
    <name>Q3YRQ1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycine oxidase</fullName>
            <ecNumber evidence="2">1.4.3.19</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.4.3.19" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304682.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0570"/>
    <dbReference type="EnsemblBacteria" id="AAZ68604">
        <property type="protein sequence ID" value="AAZ68604"/>
        <property type="gene ID" value="Ecaj_0570"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0570"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_1_0_5"/>
    <dbReference type="OMA" id="CRRLEPM"/>
    <dbReference type="OrthoDB" id="912110at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043799">
        <property type="term" value="F:glycine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="5"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="353" mass="39902" checksum="55153C2DDE19242D" modified="2005-09-27" version="1">MNKKAAVVGAGLVGRLLALRLLREGWQVTLFDKFGKNDKRSCGVIAAGMLALYSESEKMEKTIFELGVKSLNLWPRILSSILGDTSFKMLGSLIVAHRNDISDLERMIMFIKKRSCLNDLQIIDESDLKVLEPNLSFPYGLYLPGEGQIDTSQLFYNTTRTLEHYGVIWYEYAVVNEIVSRVVFVEDRKFKFDFVFDCRGIGAKNDLPNLRGVRGEVLLLYAPQVILNRPIRMVHPRYSIYIVPRRNSQFVVGATEIESSDMSEVSVQSVLELLSAAYSVHKGFAEARIIDMSSNCRAAFTDNLPKIYVQGNLVRINGLYRHGYLLTPSLIEEVILLLNENFSSNSSIIEIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRQ7</accession>
    <name>Q3YRQ7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">OmpA/MotB domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0563</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304676.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YRQ7"/>
    <dbReference type="STRING" id="269484.Ecaj_0563"/>
    <dbReference type="EnsemblBacteria" id="AAZ68598">
        <property type="protein sequence ID" value="AAZ68598"/>
        <property type="gene ID" value="Ecaj_0563"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0563"/>
    <dbReference type="eggNOG" id="COG2885">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016890_9_4_5"/>
    <dbReference type="OMA" id="TDEGWAF"/>
    <dbReference type="OrthoDB" id="1544679at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006664">
        <property type="entry name" value="OMP_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01021">
        <property type="entry name" value="OMPADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230975" evidence="3">
        <location>
            <begin position="25"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="OmpA-like" evidence="4">
        <location>
            <begin position="33"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="162" mass="18209" checksum="392C39865F18BE0C" modified="2005-09-27" version="1">MKHKLVFIKFILLCLILSSCKTTDHVPLVNTDHVFSNMKTIEKIYFDFGKATIGDSDKAILEKVIQKAQKDTNTNIVIVGHTDTRGTDEYNLELGEQRANAVKDFIIEHDKSLENRITVQSKGKSEPAVLVYSSNPEEAEHAHAKNRRVVITLTDNGNKTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRR5</accession>
    <name>Q3YRR5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNAse E</fullName>
            <ecNumber evidence="7">3.1.4.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0555</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304668.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0555"/>
    <dbReference type="EnsemblBacteria" id="AAZ68590">
        <property type="protein sequence ID" value="AAZ68590"/>
        <property type="gene ID" value="Ecaj_0555"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0555"/>
    <dbReference type="eggNOG" id="COG1530">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003468_5_3_5"/>
    <dbReference type="OMA" id="TAYRTNM"/>
    <dbReference type="OrthoDB" id="1209444at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019307">
        <property type="entry name" value="RNA-bd_AU-1/RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004659">
        <property type="entry name" value="RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30001">
        <property type="entry name" value="PTHR30001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10150">
        <property type="entry name" value="RNase_E_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00757">
        <property type="entry name" value="RNaseEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1" evidence="6">
        <location>
            <begin position="41"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="608" mass="69794" checksum="B9D58FD319CF3CEE" modified="2005-09-27" version="1">MSNHNKRILIDAVYQDGIRVAVLRDGQVVEFDQESKDKKCLKGNVYCAVVKRIEPSLQAVFIEYGANKHGFLPFSEISLDYYNISEEEKEKLYQILYEDSDIKNDDDVKSVDDSVVCDNKDQDDVNDYNEKLTLLKKDNSKGSYNKSKLPFYKLYKVQDVIKLEQKLMAQIIKEERGNKGATFSTFITLVGRYCIFMPNSGNKNSGISRRIEDADDRKTLKNFLNVIKLPKESGMIIRTAGSNKSQKEIEQDLSYLQSTWRSIQNSYVSITVPSLLYVEGDIIKRTLRDYCDENTQDIIVSGSYAYELAKECVKFMFRNRVRLRIYKGSVPIFSYYKIESQICELYGNIVNLPSGGYMVITPTEALVSIDVNSGKMTGEDSIEETAYKTNMEAVKEIARQVNLRGLSGLIVIDFIDMLKYRYCRSVESEIKEAFKYDKAKVQFGYISTFGLMEISRQRVKQSILEANTVQCSHCNGVGRVRSLESSSGDILRDIRYCANKNRNKLINISVAGQMIEYFFNNKRQHIAEIEEEFSVSVKLTVNQNTSNISDFNINVENDDISNQNKDVVNIFTVDYKISEDNKLKEEMQDTSTGSMWIKKWLTRIFSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRS9</accession>
    <name>Q3YRS9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pseudouridine synthase</fullName>
            <ecNumber evidence="5">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0540</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Responsible for synthesis of pseudouridine from uracil at positions 955, 2504 and 2580 in 23S ribosomal RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(955/2504/2580) in 23S rRNA = pseudouridine(955/2504/2580) in 23S rRNA</text>
            <dbReference type="Rhea" id="RHEA:42528"/>
            <dbReference type="Rhea" id="RHEA-COMP:10099"/>
            <dbReference type="Rhea" id="RHEA-COMP:10100"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304654.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0540"/>
    <dbReference type="EnsemblBacteria" id="AAZ68576">
        <property type="protein sequence ID" value="AAZ68576"/>
        <property type="gene ID" value="Ecaj_0540"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0540"/>
    <dbReference type="eggNOG" id="COG0564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_4_4_5"/>
    <dbReference type="OMA" id="AHPSPGW"/>
    <dbReference type="OrthoDB" id="1714699at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseudoU_synth_2" evidence="6">
        <location>
            <begin position="90"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="323" mass="36603" checksum="A82301ADE633DA19" modified="2005-09-27" version="1">MLEKKIFIKIEEANNRLDKLIANKLNTSRNQVQQLIKDSKVSLFNNIITDNNHTTKVNDIYIINIPTKEISNTIIPNHSIPISVLYEDHDIIIINKTSGITVHPGAGTKNNTLVNALVAHYGNALSTVGKATRPGIIHRLDKDTSGLMVIAKNEKSYYFLSELLLKKQIKKEYLAVVWGSPNPKNNTIQNYICVKPNNKQMMAVTHLSHKGKLAITEYKTKQNFSNIASLIKCRIHTGRTHQIRVHMSHIGNSIIGDQKYGKNNSKITKYAKQSTIINTLKRQALHAYKLGFIHPTKNTYIEFRADIPHDIQTLINELEQLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRT0</accession>
    <name>Q3YRT0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Biotin/lipoyl attachment:Carbamoyl-phosphate synthase L chain, ATP-binding:Carbamoyl-phosphate synthetase large chain, N-terminal:Biotincarboxylase,C-terminal</fullName>
            <ecNumber evidence="10">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0539</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.4.1.3" evidence="10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304653.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0539"/>
    <dbReference type="EnsemblBacteria" id="AAZ68575">
        <property type="protein sequence ID" value="AAZ68575"/>
        <property type="gene ID" value="Ecaj_0539"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0539"/>
    <dbReference type="eggNOG" id="COG4770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_3_1_5"/>
    <dbReference type="OMA" id="IGPKHYS"/>
    <dbReference type="OrthoDB" id="361205at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0092">Biotin</keyword>
    <keyword evidence="2 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="9">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="8">
        <location>
            <begin position="122"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="7">
        <location>
            <begin position="579"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="656" mass="73844" checksum="94F0A5B64F438088" modified="2005-09-27" version="1">MIKKILIANRGEIACRVMRTASKMGISCVSVYSSADVYSLHVLSAEEAVNIGPAPVNQSYLNMEKICEVAHNAGVDAVHPGYGFLSENAEFPEKLQEYNIQFIGPSPSSIRMMADKITSKKIAESAQVNIIPGYMGIVDSVDEAKSIARSIGFPVMIKATAGGGGKGMRIVKSCEEMDQAFTSATNEAAKNFKDGRIFIEKYIELPRHIEIQIIADKYGNIVCLGERECSIQRHNQKVIEETPSPFLNEETRQKMYQQCVNLAKKVGYYSAGTIEFIVDQNRQFYFLEMNTRLQVEHPVTELVTGIDIVEEMIRIADGEKLRFTQQDVKFIGSAIESRIYAEDPVKNFLPSSGRIVYYSAPMPNDNLRIDSGVFEGAEVSMFYDPMIAKVCTYGKNRDEAVSFMQKYLNEFYIGGIAHNIDFLLSVFHHPVFMSGNINTRFVEQFYSGGFEYDPLTEDCIELFVLTSLCIFFQSEYDNSLNHSCNSMDLSVYVDGQEYFVNAKYQNGKVLAIYNQNEYLVSGTWNTNFKIFKLQISEELFHVKVDSRLNKYKLRYSTMSALCVVYRSCISNLLPIMPQVSTEELCCNNVCSPISGMIVKIYVKEGEKVQPGQSLLVIEAMKMENVIYSNVKSVVKSVFFSEGNSVIVGDVIIEFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRV4</accession>
    <name>Q3YRV4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304629.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0515"/>
    <dbReference type="EnsemblBacteria" id="AAZ68551">
        <property type="protein sequence ID" value="AAZ68551"/>
        <property type="gene ID" value="Ecaj_0515"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0515"/>
    <dbReference type="eggNOG" id="COG3737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074390_2_1_5"/>
    <dbReference type="OMA" id="QIREAHF"/>
    <dbReference type="OrthoDB" id="1948784at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036748">
        <property type="entry name" value="MTH938-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007523">
        <property type="entry name" value="NDUFAF3/AAMDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192">
        <property type="entry name" value="PTHR21192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04430">
        <property type="entry name" value="DUF498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64076">
        <property type="entry name" value="SSF64076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="120" mass="13400" checksum="8EAF1FAAECA4C6E3" modified="2005-09-27" version="1">MDITPIICPNQNVITGYGTGKFLINDNIYLGSHIIFPNKIISYLNLSISNLEDIIELLDETLDIVLIGTGKNHIFLPDTVKNNFLKRGFNIEHMSTGAVCRTYNILLYEGRNVCAALISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRV5</accession>
    <name>Q3YRV5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter, family 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0514</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304628.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0514"/>
    <dbReference type="EnsemblBacteria" id="AAZ68550">
        <property type="protein sequence ID" value="AAZ68550"/>
        <property type="gene ID" value="Ecaj_0514"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0514"/>
    <dbReference type="eggNOG" id="COG1108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028808_3_2_5"/>
    <dbReference type="OMA" id="RIDLMAY"/>
    <dbReference type="OrthoDB" id="1421296at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="267" mass="29250" checksum="AC75758473FEA51F" modified="2005-09-27" version="1">MFFEIVNEYFFVNGVVAILIVSLVTGSLGSFMIWKNLSYLGDSISHASILGVALAILLNISISSGILCISIIFALLLSYSMNKIYSIDTVLSIVTNVIMSLGMILLSFFPSATSNIIHSLFGDVLMLTNKDLIIMALVSLIIITIIVYRWKYWLIISISNDLSASEGVNIGFIKLEFLIILSIFIAFAAQLVGILLITAFLVIPAAAARLISRTPLQMIIISTIISIFSGITGLLLSEKFDIFPGPLIIMVSFLLLLCMYCINRCVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRY6</accession>
    <name>Q3YRY6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0482</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304597.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68519">
        <property type="protein sequence ID" value="AAZ68519"/>
        <property type="gene ID" value="Ecaj_0482"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0482"/>
    <dbReference type="HOGENOM" id="CLU_2408610_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="92" mass="10365" checksum="CB4198A0FCF60930" modified="2005-09-27" version="1">MNGIILGNMLIGGGTASQDLDVFPCQQCFRQLFSQMKHLIEVDSSEPGEKICILNFLNSNGEWFGNEYVFAKLHHTIVDQVDGNITYVKTFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRY7</accession>
    <name>Q3YRY7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0481</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304596.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68518">
        <property type="protein sequence ID" value="AAZ68518"/>
        <property type="gene ID" value="Ecaj_0481"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0481"/>
    <dbReference type="HOGENOM" id="CLU_1576022_0_0_5"/>
    <dbReference type="OrthoDB" id="2129506at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="169" mass="19023" checksum="BA5ACE09BBBC6D90" modified="2005-09-27" version="1">MDLLLLLLNNGTPCLVPTNKCLIKKNAEGKIKSASMVDSAMNARLGTPNPQSTYAAFHNLFLSRFQRYCIMTSERLPVDMMCLFGYSGLLSKATCLFIKIPKNSFRDLLENGSVLPREFLVGCGIQAEQHDGSQYKNHAIEVFNAVKNRIQMNFNTPMSYNHILSFYDS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS21</accession>
    <name>ARGB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetylglutamate kinase</fullName>
            <ecNumber evidence="1">2.7.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetyl-L-glutamate 5-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NAG kinase</fullName>
            <shortName evidence="1">NAGK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argB</name>
        <name type="ordered locus">Ecaj_0444</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14629"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetylglutamate kinase family. ArgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304562.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS21"/>
    <dbReference type="SMR" id="Q3YS21"/>
    <dbReference type="STRING" id="269484.Ecaj_0444"/>
    <dbReference type="EnsemblBacteria" id="AAZ68484">
        <property type="protein sequence ID" value="AAZ68484"/>
        <property type="gene ID" value="Ecaj_0444"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0444"/>
    <dbReference type="eggNOG" id="COG0548">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053680_0_0_5"/>
    <dbReference type="OMA" id="EGLYEDW"/>
    <dbReference type="OrthoDB" id="901370at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04250">
        <property type="entry name" value="AAK_NAGK-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041727">
        <property type="entry name" value="NAGK-C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000264702" description="Acetylglutamate kinase">
        <location>
            <begin position="1"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00082"/>
        </source>
    </evidence>
    <sequence length="323" mass="34787" checksum="4C3E072F93C74D20" modified="2005-09-27" version="1">MKLHNVLKNNNNNKEDVGPSIEQFGGNAEWFNTTKVLSECLPYIQQFSGETFVIKYGGAAMTDRKLAESFAHDIVLLKQLGINPIVVHGGGNKINSFLEKINKKSTFINGLRVTDAETLEVVEMVLCGLVNKDITQLINKAGGNAIGLCGKDANLIEAKKVCYTYKENQSNNVEKILDMGFVGEPHEVNTDLLFFIEESDFIPVIAPVCSGENNITYNVNADLVAGALANALAAAKLIILTNVSGVTDANGNLLSEISVSDAENLIEQGVANSGMIPKLQTCIKVVKEGYGSAHIIDGRIPHVLLLELFTVHGTGTMVLGNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS29</accession>
    <name>Q3YS29_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304554.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68476">
        <property type="protein sequence ID" value="AAZ68476"/>
        <property type="gene ID" value="Ecaj_0434"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0434"/>
    <dbReference type="HOGENOM" id="CLU_1238617_0_0_5"/>
    <dbReference type="OrthoDB" id="1990723at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="223" mass="26174" checksum="5AFD8A43ECCAC323" modified="2005-09-27" version="1">MNLTLLKLKTDEYCLVPTEHCRFNETLNLYYTYDDNFNCNLAYEFTSNQCDLDNAIGTQFNDLHIVESVFSNYNYVFLYGIYRNHHIVIVVTLNKAVTLHEIFEGLKFSIDSVVHCSYCDDINGIVTTRCCEDFDHPDHIVRFKRSNTVQLFYEYIKMIVLDNSEEECIGNICFYNFVDKEGNLYYRTQFSINKHDLQQNFPSSSVESNEVSILERNAGNLRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS38</accession>
    <name>Q3YS38_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0425</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304545.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68467">
        <property type="protein sequence ID" value="AAZ68467"/>
        <property type="gene ID" value="Ecaj_0425"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0425"/>
    <dbReference type="HOGENOM" id="CLU_2600484_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021387">
        <property type="entry name" value="DUF3023"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11224">
        <property type="entry name" value="DUF3023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="79" mass="9089" checksum="DD7E4B426EC1CCD5" modified="2005-09-27" version="1">MCQILKCNKCYYIGNTEESGELQLYLVIINKNSRNPIGNSMFLLKALLPCSIVSYYSKIRGLVNLRAIIRVCIFVSKRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS70</accession>
    <name>RSMA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="1">2.1.1.182</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmA</name>
        <name evidence="1" type="synonym">ksgA</name>
        <name type="ordered locus">Ecaj_0392</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(1518)/adenosine(1519) in 16S rRNA + 4 S-adenosyl-L-methionine = 4 H(+) + N(6)-dimethyladenosine(1518)/N(6)-dimethyladenosine(1519) in 16S rRNA + 4 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:19609"/>
            <dbReference type="Rhea" id="RHEA-COMP:10232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10233"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.182"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.182" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304513.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS70"/>
    <dbReference type="SMR" id="Q3YS70"/>
    <dbReference type="STRING" id="269484.Ecaj_0392"/>
    <dbReference type="EnsemblBacteria" id="AAZ68435">
        <property type="protein sequence ID" value="AAZ68435"/>
        <property type="gene ID" value="Ecaj_0392"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0392"/>
    <dbReference type="eggNOG" id="COG0030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_0_1_5"/>
    <dbReference type="OMA" id="KEEEPYF"/>
    <dbReference type="OrthoDB" id="2030110at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052908">
        <property type="term" value="F:16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000257288" description="Ribosomal RNA small subunit methyltransferase A">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <sequence length="262" mass="30203" checksum="3DF5672E16C3AE61" modified="2005-09-27" version="1">MYHVNRIYPKKELSQCFISSSHITDKIVNYAGNISDYSIIEIGPGLGTMTYSILNKNPKRLISIEKDSRLMPIHKKIVDEFNGKYEFILSDALNIDLRTIIEPPVKIIANLPYHIATPLLMKWINYIDFFTSFTLMFQKEVADRIVAQPNNKNYSILSVLIQLLSNVYKMEDFGPEIFSPQPKVMSSVINIIVLPKPRFNVNHNKLSQVLKVTFGERRKMIRSTLKKLINNTDEILQSLNINNNLRPENLSIEQFCKIANSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS79</accession>
    <name>Q3YS79_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0383</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304504.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0383"/>
    <dbReference type="EnsemblBacteria" id="AAZ68426">
        <property type="protein sequence ID" value="AAZ68426"/>
        <property type="gene ID" value="Ecaj_0383"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0383"/>
    <dbReference type="HOGENOM" id="CLU_2092976_0_0_5"/>
    <dbReference type="OMA" id="FENKMED"/>
    <dbReference type="OrthoDB" id="1870258at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="116" mass="13894" checksum="44C02EAF43B3873A" modified="2005-09-27" version="1">MTENFHNKEKAFIQQKKLDEFSYTIDDIMTKYQIKFENKMEDITSNFLMNFQHSLEQELISLIKKIYSHNSQKLNKYLIEQLLNPSSLQSLNQHEKDIIAKIFNKISFSILENLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSB2</accession>
    <name>Q3YSB2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide chain release factor 2</fullName>
            <shortName evidence="4">RF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">prfB</name>
        <name evidence="6" type="ordered locus">Ecaj_0350</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="4">Methylated by PrmC. Methylation increases the termination efficiency of RF2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0350"/>
    <dbReference type="EnsemblBacteria" id="AAZ68393">
        <property type="protein sequence ID" value="AAZ68393"/>
        <property type="gene ID" value="Ecaj_0350"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0350"/>
    <dbReference type="eggNOG" id="COG1186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_6_0_5"/>
    <dbReference type="OMA" id="YVFHPYQ"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00094">
        <property type="entry name" value="Rel_fac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004374">
        <property type="entry name" value="PrfB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00020">
        <property type="entry name" value="prfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0488">Methylation</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="5">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="4">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="335" mass="38042" checksum="64D573CE0A03834C" modified="2005-09-27" version="1">MLVDLNVQCSSTSLWTDNALAQNILSERARVSKIITSFTELEEEFNYYTELLDVVKNDTDELLNDIEIGLTKLKERIQKKEVECLFSNEADQNDCFIEIQSGAGGTESNDWSCMLLRMYVRWAEIYHNFTTEIVYKLDGEEVGIKSATIKVTGDNAYGWSKTESGIHRLVRISPFNSNAKRHTTFSSILVSPITDENINIETLDKDLRIDTYRASGAGGQHVNKTESAVRITHIPSGIVVQCQNNRSQHQNLAEAHNLLKSRLYQLKLQEKEKKMAEEHNKRCNIGWGNQIRSYIMHPYQMVKDVRTGHEIGNVNSVLDGNLDPFIISALTAKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSB5</accession>
    <name>Q3YSB5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0347</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304468.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68390">
        <property type="protein sequence ID" value="AAZ68390"/>
        <property type="gene ID" value="Ecaj_0347"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0347"/>
    <dbReference type="HOGENOM" id="CLU_753843_0_0_5"/>
    <dbReference type="OMA" id="QHANNAH"/>
    <dbReference type="OrthoDB" id="2109728at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="354" mass="39956" checksum="E6C17CF8B7EBB013" modified="2005-09-27" version="1">MEVNYKTIIGIVIGIAIAALITIAVLTVLNVIPIAVLLQLSTVFASAIAGSVVVYAKEKIFAWYDKQQYEDRASTSFKMVDKVLSASDEKIAKSSDKVVLSNDTISNYSNYPFFPEFHKNFNKILDKLSLDVRFASINQELFYSSLSPQEQTLAILSARLICKQPEMYLIYSEPVRVCIAAIAQLKIDKKFEGRARNNIISLLRKQVRISNYNVLEQELKGVMLKHTKDPVFMKLLHGLIDIDEFSGKKQIMNLVNLFSSYKYYSIYSDSAVEYYSDMASELLKQINNKQESAVDVDAKSSNVPKHKTSDNEKSIVDKRTSNSSVNACDTNNQELENNFSSKITRINSTKMRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSB6</accession>
    <name>Q3YSB6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304467.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68389">
        <property type="protein sequence ID" value="AAZ68389"/>
        <property type="gene ID" value="Ecaj_0346"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0346"/>
    <dbReference type="HOGENOM" id="CLU_525547_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="518" mass="56473" checksum="A8B18FECA5607722" modified="2005-09-27" version="1">MMSRPVAQTLCTTHVGSRATAGSSSCTASSVATDRPKIDTSGRKQSKPITKASSTTHVDSRTRTSSSIASSVTGIPKASAGSRMMSRPVTQTLCTTHVGSRATAGSSSCTASSVATGIPKIDTSGRKQSKPITKASSTTHVDSRTRTSSSIASSVTGGPKASADSRMMSRPVVGHSSTMDGGSSAQASTSGSTLGVLSGRDQTNADTAGPFELSSTVRFDSRTQTMSSDYPAYSMTGEPETGTSIGKRSEPIANPLYRRMGRKPNIPLDINLPCGRDVYTVIPSREDLEDLRERKFEYKGYVGAMSSVASRIYDVYKTSKEHAYITYPSFIKILINVLEYKDMVEDRVEVLLEDAVTDDKVWESIDDERKQKIVFCAKVIHTCPLLSVYFACIRGHVARLMSKDIDLSSLSSAFVDQVYDSLNEVEIYGTVNEYVYETKKLLAEYVEFLKNGDIRGVDLKADSMIMQGMECSQELFAHDMVNTVRKVRSNLRRFCKHPVLNHVDVVSVQTYLNKNLEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSC2</accession>
    <name>LEPA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor 4</fullName>
            <shortName evidence="1">EF-4</shortName>
            <ecNumber evidence="1">3.6.5.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal back-translocase LepA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lepA</name>
        <name type="ordered locus">Ecaj_0340</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.n1" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304461.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSC2"/>
    <dbReference type="SMR" id="Q3YSC2"/>
    <dbReference type="STRING" id="269484.Ecaj_0340"/>
    <dbReference type="EnsemblBacteria" id="AAZ68383">
        <property type="protein sequence ID" value="AAZ68383"/>
        <property type="gene ID" value="Ecaj_0340"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0340"/>
    <dbReference type="eggNOG" id="COG0481">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009995_3_3_5"/>
    <dbReference type="OMA" id="MVQIAIQ"/>
    <dbReference type="OrthoDB" id="182107at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="PTHR43512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224759" description="Elongation factor 4">
        <location>
            <begin position="1"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="4"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00071"/>
        </source>
    </evidence>
    <sequence length="598" mass="66867" checksum="34D6AEE26F4893EE" modified="2005-09-27" version="1">MDESNIRNFAIIAHIDHGKSTLADRLIEECNGLDKREMKDQVLDSMDIERERGITIKAQTVRLVYKAKDGQTYYLNLMDTPGHVDFSYEVSRSLAACEGSLLVVDSSQGVEAQTLANVYKAIDSDHEIIPVLNKIDLASSDPEKVKLQIEDMIGIDASESLLVSAKSGVGIQDVLEAIVSKLPSPCGKSDSPLKAILVDTWYDTYLGIVILLRIKDGVIKKGMKIVMMSNNAVYQVDNVGIFTPHKKIVDQLSVGEIGFITASIKELSDCKIGDTITEEQRRCDAPLPGFRTSHPVVFCSIFPNEAGEFDRLREALKKLQLNDASFTFEIEVSNALGYGFRCGFLGMLHLEVIQERLEREFNLDLTATAPGVIYKVTTKSGEIREVHNPHDFGETQDIANIKEPWICATIMVPDQYLGVIMSLCNNKRGEKLDLSYSGNTALLKYRLPLSEVVFDFYDRIKSLSKGYASLDWEMDEYLDSEIAKLSILINSEPVDALACIIHKSKVEQRGREICLRLKDLIPRQQYKIAIQAAIGGRIIARETISPYRKDVTAKLYGGDVTRRMKLLEKQKKGKKKLRSIGNVNVPHNAFIQALKIID</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-29" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSC4</accession>
    <name>PNP_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polyribonucleotide nucleotidyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polynucleotide phosphorylase</fullName>
            <shortName evidence="1">PNPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pnp</name>
        <name type="ordered locus">Ecaj_0338</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + RNA(n+1) = a ribonucleoside 5'-diphosphate + RNA(n)</text>
            <dbReference type="Rhea" id="RHEA:22096"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyribonucleotide nucleotidyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304459.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSC4"/>
    <dbReference type="SMR" id="Q3YSC4"/>
    <dbReference type="STRING" id="269484.Ecaj_0338"/>
    <dbReference type="EnsemblBacteria" id="AAZ68381">
        <property type="protein sequence ID" value="AAZ68381"/>
        <property type="gene ID" value="Ecaj_0338"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0338"/>
    <dbReference type="eggNOG" id="COG1185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004217_2_2_5"/>
    <dbReference type="OMA" id="LHILDVM"/>
    <dbReference type="OrthoDB" id="122725at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004654">
        <property type="term" value="F:polyribonucleotide nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01595">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012162">
        <property type="entry name" value="PNPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015848">
        <property type="entry name" value="PNPase_PH_RNA-bd_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036456">
        <property type="entry name" value="PNPase_PH_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252">
        <property type="entry name" value="PTHR11252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03726">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005499">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46915">
        <property type="entry name" value="SSF46915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03591">
        <property type="entry name" value="polynuc_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000329630" description="Polyribonucleotide nucleotidyltransferase">
        <location>
            <begin position="1"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="557"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="626"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="714"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="714"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="753"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="796" mass="87407" checksum="8C536477CF05F02C" modified="2005-09-27" version="1">MFNLIRKSTEWGGKTLILESGKIARQANGAVMVSYAGTTVLATVVTGKTKEPVDFLPLTVQFVAKSYAVGKIPGGFLKREGKPSDRETLISRLIDRSIRPLFPSGFYDEVSIVCNLLSYDTVTPPEVTALIGATAALSISGVSFNGLVAGVRIGYLPSEDKYLLNASADDMLYSSLDLFLSGNEDSVLMVESEASELSESQMLRAVTFGHQNCQEVINLIREFSEEKGVQPIEFIPHDINPIVNYIESSYKQDFSLAYSNTVKKERVLKLEELRDKVLSDFAEKCSVDNVECDNQDVISALKSFERSLVRSQIVETSSRIDGRAFDEIRNIEIEVDVLPKAHGSALFTRGNTQALVVTALGTPQDEQIVDDLDGDRRENFLLHYNFPPYAVGESAALRAPGRREIGHGKLAWRAIRYVLPEKSDFPYTIRVVSEITESDGSSSMATVCGASLALMDTGVPIKSPVAGIAMGLIKEGDKFIILSDILGDEDYLGDMDFKVAGTAEGVTALQMDMKISGISVDVIEKALLQAKDGRMHILSKMNAVIQESRNSIKNHAPRIESIFINKDKIRNVIGSGGKNIRDICEKTGAKIEIIQDGTVMIYAVNNEAVEYAKSMIMDIVTEPEIGKVFEGTVVEIVKFGAFVNFLGGKRGLIHISEIKNEHISAVGSVISVNDKVKVLVIGIDREHVQLSMRRVDQETGEPIDGELYNVRKSSFSDDSSSSGTSSSGSSFKESYSGNRHGSHEKRRSGGSRSSRRNSSGSNYYREDLHSSDFGNNNRSFSNSRNGHEVPRKPRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSD7</accession>
    <name>Q3YSD7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304446.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0324"/>
    <dbReference type="EnsemblBacteria" id="AAZ68368">
        <property type="protein sequence ID" value="AAZ68368"/>
        <property type="gene ID" value="Ecaj_0324"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0324"/>
    <dbReference type="eggNOG" id="ENOG50332EE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170191_0_0_5"/>
    <dbReference type="OMA" id="QAHKEIT"/>
    <dbReference type="OrthoDB" id="1644472at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021251">
        <property type="entry name" value="DUF2793"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10983">
        <property type="entry name" value="DUF2793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="117" mass="13907" checksum="E6775F8E9350195F" modified="2005-09-27" version="1">MMTQSVNLKFDLVMHNQAHKEITVNEALIKIDMLMNNCILELQCNTPIKEISSGDMYIIGPTPTIQEWKNKQNYVTYYYINQWYFIQPNTGLTLWIKSRNQLYTYNNNQWIPSYSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSF9</accession>
    <name>Q3YSF9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Inositol monophosphatase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304424.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0302"/>
    <dbReference type="EnsemblBacteria" id="AAZ68346">
        <property type="protein sequence ID" value="AAZ68346"/>
        <property type="gene ID" value="Ecaj_0302"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0302"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_0_0_5"/>
    <dbReference type="OMA" id="CLAIIED"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="255" mass="28874" checksum="FB7F75EDE59C4CA0" modified="2005-09-27" version="1">MSVIFSSVTNIMLKSVRQSSRALIRDFSEIRYMQSSYTAASNFTRSAYLRSKKIITDGLYNYRQDYGVVFDDTRDVSDVGENFWFVNPIDSKTNFISYLPYFATLIAFFRQGEPVAAIIDAPILKETFYVERGVGAFSENVQSRYIKLHVGNKQTVSRAVIDFVITHLSIDMIDSQLNSQAVIIRAMGSMALGFAYLCSASYDALIYSNLNKYQAAVGKLFVEESKGKIVCDNELFIASNFALCDYLRKKFNSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSG6</accession>
    <name>Q3YSG6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="7">DMRL synthase</shortName>
            <shortName evidence="7">LS</shortName>
            <shortName evidence="7">Lumazine synthase</shortName>
            <ecNumber evidence="4 7">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ribH</name>
        <name evidence="8" type="ordered locus">Ecaj_0292</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="4 7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304417.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0292"/>
    <dbReference type="EnsemblBacteria" id="AAZ68339">
        <property type="protein sequence ID" value="AAZ68339"/>
        <property type="gene ID" value="Ecaj_0292"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0292"/>
    <dbReference type="eggNOG" id="COG0054">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089358_1_2_5"/>
    <dbReference type="OMA" id="CQGVTQG"/>
    <dbReference type="OrthoDB" id="1680292at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09209">
        <property type="entry name" value="Lumazine_synthase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="PTHR21058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 7 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="47"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="73"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="1-deoxy-L-glycero-tetrulose 4-phosphate binding" evidence="7">
        <location>
            <begin position="78"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="148" mass="16208" checksum="67ED487C97DF4549" modified="2005-09-27" version="1">MSNEYNILIVVSQFYPEITNLLLDGAVDQLKKYSKPVKHDVVYVPGSFEIPAAIVFAIMSDKYKYDGYIALGCVIKGETDHYYYVCKGVAGSLSEIAANHAVPMGFGVITAENLELAMVRADMHKKNVGGNAAKAALTMIELHNKFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSI2</accession>
    <name>Q3YSI2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translation factor SUA5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304401.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0276"/>
    <dbReference type="EnsemblBacteria" id="AAZ68323">
        <property type="protein sequence ID" value="AAZ68323"/>
        <property type="gene ID" value="Ecaj_0276"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0276"/>
    <dbReference type="eggNOG" id="COG0009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_3_2_5"/>
    <dbReference type="OMA" id="LVDAFWP"/>
    <dbReference type="OrthoDB" id="1913202at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00057">
        <property type="entry name" value="TIGR00057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YrdC-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="198" mass="21913" checksum="DF85D701FFFCDEAA" modified="2005-09-27" version="1">MILKAIQALNNKHLIAFPTETVYALAGDAYSIEAIQKIYQIKGRSYNKPLSLLVGNINKIKQFSNLTDHAIKIIQKLSPGPITFVLPIHNYHKLPRQFFNNTIGIRIPNHPIALEIVNKIENPVVGTSINISGQPSVTAAHQIHETIKKHISVTIEDDSLVNGTESTVIDLTSYKILRAGAVAEKKLLTIIQNILQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSI7</accession>
    <name>Q3YSI7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0271</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304396.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68318">
        <property type="protein sequence ID" value="AAZ68318"/>
        <property type="gene ID" value="Ecaj_0271"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0271"/>
    <dbReference type="HOGENOM" id="CLU_905327_0_0_5"/>
    <dbReference type="OMA" id="ESITHQP"/>
    <dbReference type="OrthoDB" id="1330045at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="329" mass="37460" checksum="B7A323248A4D7C4C" modified="2005-09-27" version="1">MFYPKRDLKTIYSKDNTLSAEHLLELMNSSSKELKEYRSQLLTGYVKKEEKKLTDQNKTPEEIQKHISSPEFFYKASSYADYKVNHGIEMFLISQIGNKVKILNQRIKSLGYDRQSMILHGMYKISLLTEINSIIKDMSDDDFKELLEVIISCSHLKTDLTEQQQQSRQAAVAEYVKESKGFSMEKVREKAEELTGIDIANPIASLRKKFGADKPEGKQHMVSMDEKLVQTVINPFNSYYFILGALAIGLEGFIPPLVPILLIAITGKSLFSLVTEDKDKEGSVHSPFQEGIIRQPVTAEGQQNYTAMLERQKKEESEQQSTSPTSKKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSJ0</accession>
    <name>GATB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatB</name>
        <name type="ordered locus">Ecaj_0268</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304393.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSJ0"/>
    <dbReference type="SMR" id="Q3YSJ0"/>
    <dbReference type="STRING" id="269484.Ecaj_0268"/>
    <dbReference type="EnsemblBacteria" id="AAZ68315">
        <property type="protein sequence ID" value="AAZ68315"/>
        <property type="gene ID" value="Ecaj_0268"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0268"/>
    <dbReference type="eggNOG" id="COG0064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019240_0_0_5"/>
    <dbReference type="OMA" id="ARKWWMG"/>
    <dbReference type="OrthoDB" id="497127at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241219" description="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B">
        <location>
            <begin position="1"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <sequence length="482" mass="54735" checksum="AF73466D6AE462B2" modified="2005-09-27" version="1">MRVIKGNKCDWEVVIGLEVHAQVISNSKLFSGASAKTYDAPPNTQVSLFDVAMPGMLPVLNEYCIYQAVKTGIALSCKISKYSAFDRKNYFYPDLPAGYQITQFYYPIATEGKVVLEDHNAKEVRIARIHLEQDAGKSIHEFSQTYIDFNRAGVALMEIVSEPDLRSVEEVAEYLKKLRMILRFIGTCDGDMEKGSFRCDANVSVRPFGSNELGVRSEIKNLNSIRYVMQAIEYEANKQVNILENNEIFTQNTLLFDVSLGQTRVIRTKEDAHDYRYFPDPDLFPVKIDDQYIDHVKSLLPELPLQKRDRYINDFNLNKSDADILSSDKDVAMYFEKVVEKHDARLAASWIIGELFGRLNKLGITIDESSVKAEHLIQLLDLIVDNTISGKIAKQVFDMMFESGKLPALIVSEHGLKQVDDADVLYVVVEKILKDNASKVEEYKQGKEKLFGYFVGQVMKETQGKANPEMVNSIIKQQLESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSJ5</accession>
    <name>Q3YSJ5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDDEXK_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304388.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0263"/>
    <dbReference type="EnsemblBacteria" id="AAZ68310">
        <property type="protein sequence ID" value="AAZ68310"/>
        <property type="gene ID" value="Ecaj_0263"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0263"/>
    <dbReference type="eggNOG" id="COG2887">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3893">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_310724_0_0_5"/>
    <dbReference type="OMA" id="ALWWPRF"/>
    <dbReference type="OrthoDB" id="1283891at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDDEXK_1" evidence="1">
        <location>
            <begin position="661"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="911" mass="106196" checksum="DEA19665AF756970" modified="2005-09-27" version="1">MRKVFSVYPAESFLTVVAKFVFDVFNDTFSNVIIIVPNQEDVELLCNELKLYFSDTVFGKIKIMSLADIDQDLLVGNHGSIKIMSPIRKLLLLMEFIKLWNSENDEDYPLTMSYELATLLNEINVHCIELSNLENLFDCDLPVHCQKTAKFLFRLSKKWREVLKEEGVLDILEHRSLYINSLIEYLEKESLNSTVIFAGMAFDEFFINFIQALYKLSNTKIILPYVDMTICQSEWELLDECHYQYCIKNVLDVLMINRDDIIFLGSPSSSIFVNSVFNFNLFLEAYHRNEIQNYDDYNRVKLITCVSDEDEAQIVSTLVKENSCNDLTIFTNNLLLSKRINSILDSQNLLDKSDNSYLILSFVLYILEVAISNWNPISLLSLLKHPFVTLEYVKEDYEALVSDFELKVIRCYSSCDFASIESNIKEHVPDLLFFWEKITSIMLPLVNVRYEKISSIVKAHITCMNNLLKGNTVVSLNDSNKIEEFFNSLKNSCNGIKIYGIDLYYEILLSILRSNFFSENYKLSCLNLSKKKVVIFAGFNEINYGVNSFNTLLNGWIRAKLGLPSIQKERGYFAYLLHSFFYADKIYITQSLKSFGKINEESVWVRRLKILGELYDIEDLKCAMHLRTQDKSLFDDVKNLVYLRPNPNPELDKRLGAFNILSSTALETLVKNPYVFYLSSILNILPCRGINEKFSMRDFGIIVHNILHKYLLNNKSKCQCKDLVEIAVSELLDKYKNFPQVETILWPKFQKIAEQFFEMNLERDHYIDEIMTERFFSWKINSNIKVVSKCDRVECLRDGSVVIIDYKTGMIPSQSDINYGISLQMIIQALTVKQSLKKDISGLMYWKMSSEDMKIISIDNYVELMKQLEIDLEQLILNYVTFMKPFTASCDMSRYTDYDLLTRIKEWMYFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSM1</accession>
    <name>Q3YSM1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endonuclease III</fullName>
            <ecNumber evidence="5">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nth</name>
        <name evidence="7" type="ordered locus">Ecaj_0235</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304362.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0235"/>
    <dbReference type="EnsemblBacteria" id="AAZ68284">
        <property type="protein sequence ID" value="AAZ68284"/>
        <property type="gene ID" value="Ecaj_0235"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0235"/>
    <dbReference type="eggNOG" id="COG0177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_3_0_5"/>
    <dbReference type="OMA" id="QIIWYGR"/>
    <dbReference type="OrthoDB" id="1381897at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01083">
        <property type="entry name" value="nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="6">
        <location>
            <begin position="38"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="212" mass="24069" checksum="E02C94DB90CD8C96" modified="2005-09-27" version="1">MNERKIDLLFSKFQGHNPHPKIELRYTNDFTLLVAIVLSARTTDVSVNKITSRLFKIVDTPKKMLDLGENGLKGYINTIGLYNAKSKNIIALSEIIINQYNGAVPLDFNALVELPGVGRKSANVFLNTWLKLPSVAVDTHVFRVSNRIGLVNENNVLKTEYALLNVIPKKWLLYAHHWLVLHGRYICKSRKPLCNQCIVKDLCEYESKSCCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSM4</accession>
    <name>Q3YSM4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304359.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68281">
        <property type="protein sequence ID" value="AAZ68281"/>
        <property type="gene ID" value="Ecaj_0232"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0232"/>
    <dbReference type="HOGENOM" id="CLU_666885_0_0_5"/>
    <dbReference type="OMA" id="TEDNINQ"/>
    <dbReference type="OrthoDB" id="1739925at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="412" mass="47396" checksum="C94D46538906E5DF" modified="2005-09-27" version="1">MLSLFRNITQRLSKPSFMQALDRFNIQKIRNTKYDVCRTGEQAWKDIPRDSILVNRLSTNLTKLIADFNRSNKKPLEGDEHRIILHSMFNSTFNTIANKQVHPLLIDECITMMNQTSLHGSLCTELMRYIGEDIQKRIFPENSILPAPKNFDTKIEVISNTELRVEYNENIDFRDIASGNSVCRSTGTLTFNINVDQYNLITYNNPKIKLELPTQISSQLCSPTMVFVRNMFTPLRNSIRRVKSILTRSPFNSTNKYQIKNNDTISTIEYNIPDYTCTSVLLTQHRLRNTIENTKEDITDKEVEESSRHSQKPRAQDHEEQNNKITVSAMVHPVPSTPPITEEINELEDIQKENTEDNINQTSLSALKTDNQHQQDSEADNQNTQDTVTPNTKLNKQKAQLFGSPKTTSRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSM6</accession>
    <name>Q3YSM6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304357.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0230"/>
    <dbReference type="EnsemblBacteria" id="AAZ68279">
        <property type="protein sequence ID" value="AAZ68279"/>
        <property type="gene ID" value="Ecaj_0230"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0230"/>
    <dbReference type="HOGENOM" id="CLU_561102_0_0_5"/>
    <dbReference type="OMA" id="DEDIKCA"/>
    <dbReference type="OrthoDB" id="2105757at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="486" mass="56425" checksum="D0FBC6DCD4CE0C6E" modified="2005-09-27" version="1">MHDNNIRYALAAIVSLLILLLVVLVVLVFRMIYTYNCHQLHQYNTLHQDNTISIYPKNISRSLVQNIISISDHHKQQVITGEALGSYVTSPSNGQTPCDQDIKPHILIHDTHYKTYSYFQDAHELFIKNTRKYTSYLFRILKTPEQHSSHCFNNTYHHRIGWSAILDTMFTVFCKTSTDQDFLPPRPEIIQEIITNLRWDGYNILAYKTLYHMLHKTPNITKINISQNSNITKLNFRLTNTRDRIKVDITTKVLLSTAETLTIKYPIDITFSFYISCTSQGSNYSICYQDTNLKLYIPIKLADCINTGPIINDIPEDPNYAMLANKQQISDTQYLIEYKLPNLVITPLLPKQNYHYLELQEQKVQEGEQTTKTKQEVSVREKISNIQDILKHKLLNDITTQSLPQQDYQESQKQKEQAANTRKKPLQVLGDASSHFITTQGRNYFSQQQRHAKKQITEIKQVANAAKKILLQPFGDAYSYFIVNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSN1</accession>
    <name>Q3YSN1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-demethoxyubiquinol 3-hydroxylase</fullName>
            <shortName evidence="3">DMQ hydroxylase</shortName>
            <ecNumber evidence="3">1.14.99.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">coq7</name>
        <name evidence="4" type="ordered locus">Ecaj_0225</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the hydroxylation of 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol + AH2 + O2 = A + a 3-demethylubiquinol + H2O</text>
            <dbReference type="Rhea" id="RHEA:50908"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="1.14.99.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="3">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COQ7 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.60" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304352.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0225"/>
    <dbReference type="EnsemblBacteria" id="AAZ68274">
        <property type="protein sequence ID" value="AAZ68274"/>
        <property type="gene ID" value="Ecaj_0225"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0225"/>
    <dbReference type="eggNOG" id="COG2941">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071892_2_0_5"/>
    <dbReference type="OMA" id="HYNDQVR"/>
    <dbReference type="OrthoDB" id="1382712at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008682">
        <property type="term" value="F:3-demethoxyubiquinol 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01042">
        <property type="entry name" value="DMQH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01658">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011566">
        <property type="entry name" value="Ubq_synth_Coq7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237">
        <property type="entry name" value="PTHR11237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03232">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="170" mass="19244" checksum="25ECDF4442A16A8A" modified="2005-09-27" version="1">MLQENSLLEQSIRVNHAGEYGAVCIYSGQELVFRKMSIGKKISEMKKQEQQHFSYFNSVMKDRKIRPTVFLPIWHVMGVMLGITTALIGEDAAMACTVAVEEVISDHYQDQITTLDDGELKDKIIQFRNEEIEHHDTAMCHNSGNAGNYNVLSFFIKKACKVAICISKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSN3</accession>
    <name>Q3YSN3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304350.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0223"/>
    <dbReference type="EnsemblBacteria" id="AAZ68272">
        <property type="protein sequence ID" value="AAZ68272"/>
        <property type="gene ID" value="Ecaj_0223"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0223"/>
    <dbReference type="HOGENOM" id="CLU_103734_1_1_5"/>
    <dbReference type="OMA" id="WFNDFMH"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="235" mass="26919" checksum="0D707995454F5AF0" modified="2005-09-27" version="1">MLVSWLQILIKIIGKILPTRLISTFLGTGYLPAWQSHWAAVLSLILAYILFYLIYGMYYVQYGLLVTAAVIAAFFFKISLVLVVVAIISIFIFQSHSSADSRTDIIVVQIALGQLLVVALAMPAILAVYNYLSIFYGRICEGIFICPTWFNEFMRFLIFFLIPFVFFNIIEIIKPWPISNFQISYNNCFSIVSEGLILVLYTLLVMYLAAFICFDLTVHDAVALNEYIFTLMRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSN5</accession>
    <name>Q3YSN5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ankyrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304348.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0221"/>
    <dbReference type="EnsemblBacteria" id="AAZ68270">
        <property type="protein sequence ID" value="AAZ68270"/>
        <property type="gene ID" value="Ecaj_0221"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0221"/>
    <dbReference type="eggNOG" id="COG0666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_313696_0_0_5"/>
    <dbReference type="OMA" id="YNAKRCA"/>
    <dbReference type="OrthoDB" id="345313at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="SSF48403"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="719"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="757"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="933" mass="104424" checksum="0016D6B73DB15AB3" modified="2005-09-27" version="1">MLNNGTSISQDANDDIVDSLSDNNDGHSKIVLSELDLAKLNQVKTQGIPSNRLHPLNRLLADASLTNNFSTFFKELSSFLRCGKKKRIKQCCQFVIFKNEKGHYSIEGSVSSIYSNHALAKQVLQGQLYVCDLGSLALVFPDNSEEREAFLTLIFNSTFSQKKTEISEFEIKCFSNDVVNYNAIHFNTESLQYALEHEDVQAVKVISGFLNKLKERDNVLKRVIENALIKVIQQRNNVSDNLLECINCLLDIKIESFDLDAEENQLVAEKLRHFTRIVCGDDVLLSRICKAAYENIVKVVCLVELEEAKRIKSEYVKSGLLETYYYHDRDMRLVNMYYGPDKDNILFVLVQSDSIGLLRLLFEKYKLDVNTVNAYGYTVLHFAALYNAKRCAKFLLESESKIFHQPAKSQLTPFHLAAGSNALSVLKLLARHKPDAIYDKDSMGRNIMHHAAIGGALDTLLYSLSLGIDVNAQAIVRDDNGEIDELMNAGRSCTALHFAIEEGKLGVVNCLLSCRDIDLSIENSEGCTPITTVDHNGGNLLHQCVLFNDVGLLTKVLRLGWQGVILEKNCAGQTAFDLAIKHGRTVCAGLLLPILCEQLKCKGSEWKKIFENSDSNGDTLLHQAILSRNVTLVKKILSFHDIRLMDVNKDNETPLDIAIKVGSAEIISLLIKDNRQSLISESGSSYIMKLMNKGLMTRGIYQIIKSRCLKKKRCSYAKLGVSFILTFVLITCIAVFCILKYNQELQNAFGIDGNTQIFFIAITVFLLSVICVGCCLFATYHIRLTEREKLLRIEISDTHFSTAANSSVSAASVSSEYIDARQSSSASSTEGSGEREMQNEIVKTHQDASKLERLTLKNNAFTEGSGEREMQNEIVKTHQDASKLERLTLKNNALQERCGNMHRRPSKLIHPQVLSSTELAEQGRCDITLTSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSP2</accession>
    <name>Q3YSP2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetylornithine aminotransferase apoenzyme</fullName>
            <ecNumber evidence="4">2.6.1.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0214</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304341.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0214"/>
    <dbReference type="EnsemblBacteria" id="AAZ68263">
        <property type="protein sequence ID" value="AAZ68263"/>
        <property type="gene ID" value="Ecaj_0214"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0214"/>
    <dbReference type="eggNOG" id="COG4992">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_5"/>
    <dbReference type="OMA" id="DVFPRFA"/>
    <dbReference type="OrthoDB" id="572533at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="391" mass="42669" checksum="B1B6EA03BF024D11" modified="2005-09-27" version="1">MSNSPLLPVYSPSSVTFSHGKGVYLYGYDGRKYLDFHAGIATSSLGHAHPVLVNALKTQGEKLWHLSNLHTIPEAIKLAQKLVDISFADKVFFNNSGAESVDCCIKIARSYQCGKGNNQRYRFITMKGSFHGRTYAACSANEPSKFSPLLKPYVEWFDSVNPDITSIKNAINEHTGAILLEPIQGEGGINVLDSSFLKELRAICDQNDILLIFDCVQCGAGRTGKFFAYEHVGVTPDICCIAKGLGGGFPISASLSTNNAAQFMSVGMHGSTFGGNPLATTIGMVVVEEILKDGFLENVTKNGHYLYKKLKDLANKFPIIEQVKGKGLMIGMKFNTNVNARELMYELIDYGLLTSTASGNVIRVIPPLIINQQELDEGVAILEGYLKSKYS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSP9</accession>
    <name>TRHO_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA uridine(34) hydroxylase</fullName>
            <ecNumber evidence="1">1.14.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA hydroxylation protein O</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trhO</name>
        <name type="ordered locus">Ecaj_0206</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes oxygen-dependent 5-hydroxyuridine (ho5U) modification at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + O2 + uridine(34) in tRNA = 5-hydroxyuridine(34) in tRNA + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:64224"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:13381"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136877"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrhO family.</text>
    </comment>
    <dbReference type="EC" id="1.14.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304334.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSP9"/>
    <dbReference type="SMR" id="Q3YSP9"/>
    <dbReference type="STRING" id="269484.Ecaj_0206"/>
    <dbReference type="EnsemblBacteria" id="AAZ68256">
        <property type="protein sequence ID" value="AAZ68256"/>
        <property type="gene ID" value="Ecaj_0206"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0206"/>
    <dbReference type="eggNOG" id="COG1054">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038878_0_1_5"/>
    <dbReference type="OMA" id="CDTHTNC"/>
    <dbReference type="OrthoDB" id="684577at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00469">
        <property type="entry name" value="TrhO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020936">
        <property type="entry name" value="TrhO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040503">
        <property type="entry name" value="TRHO_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43268">
        <property type="entry name" value="PTHR43268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17773">
        <property type="entry name" value="UPF0176_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000242920" description="tRNA uridine(34) hydroxylase">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="121"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00469"/>
        </source>
    </evidence>
    <sequence length="274" mass="31493" checksum="A465D8A3921337B2" modified="2005-09-27" version="1">MGYVVSTFYRFVHLSNYYDIQSVLKEFCVQHDIKGTIILAEQGINATIAGAQSALNQFFSFLDLDNRLKDIQHHESFSTHNPFSKMKVKLRNELVRLGIENFDNSVCGEYVSPQNWDDLISRSDVYTIDTRNTYEINFGKFKNAINPQTKCFRDFPEWAVSWASDKVNQDPIIAMYCTGGIRCEKSTAFMKNLGFNKVYHLKGGILEYFKSTKNTNNLWEGDCFTFDDRIVVDDNLVPGRVKCVSCDVHVTREEMKSVTRGNVLCFNCREVAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSR1</accession>
    <name>Q3YSR1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosomal DNA replication initiator-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304322.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0193"/>
    <dbReference type="EnsemblBacteria" id="AAZ68244">
        <property type="protein sequence ID" value="AAZ68244"/>
        <property type="gene ID" value="Ecaj_0193"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0193"/>
    <dbReference type="eggNOG" id="COG0593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072265_0_1_5"/>
    <dbReference type="OMA" id="DRWPDWP"/>
    <dbReference type="OrthoDB" id="1110566at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_DnaA" evidence="1">
        <location>
            <begin position="81"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="218" mass="25665" checksum="FF353E8FFA056B45" modified="2005-09-27" version="1">MQLTLFDLEDSYSYHYHDYILLEQNKATYNMLMNQEWNSFILYGKSGSGKTHLAHIWQKLKNAIFIDQYLINTKEDIGDIISNNNAFIIENIDNIDNELSILHYYNYIKENKKLLLMTSSIAPRLLNYHIKDLKSRILHTMSAKLANPDEELLKIMLIKLFADKQIHIELKVINYILNNTERSFQNLSNIVKCIDRNLPYYNNGVTIPFVKSIIEKND</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-24" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSS1</accession>
    <name>PDXH_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxine/pyridoxamine 5'-phosphate oxidase</fullName>
            <ecNumber evidence="1">1.4.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PNP/PMP oxidase</fullName>
            <shortName evidence="1">PNPOx</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxH</name>
        <name type="ordered locus">Ecaj_0183</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + O2 + pyridoxamine 5'-phosphate = H2O2 + NH4(+) + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:15817"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>O2 + pyridoxine 5'-phosphate = H2O2 + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:15149"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxamine 5'-phosphate oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304312.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSS1"/>
    <dbReference type="SMR" id="Q3YSS1"/>
    <dbReference type="EnsemblBacteria" id="AAZ68234">
        <property type="protein sequence ID" value="AAZ68234"/>
        <property type="gene ID" value="Ecaj_0183"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0183"/>
    <dbReference type="eggNOG" id="COG0259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032263_2_3_5"/>
    <dbReference type="OMA" id="PEPNAMV"/>
    <dbReference type="OrthoDB" id="1542844at2"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00304"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00305"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004733">
        <property type="term" value="F:pyridoxamine-phosphate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01629">
        <property type="entry name" value="PdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000659">
        <property type="entry name" value="Pyridox_Oxase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019740">
        <property type="entry name" value="Pyridox_Oxase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019576">
        <property type="entry name" value="Pyridoxamine_oxidase_dimer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851">
        <property type="entry name" value="PTHR10851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10590">
        <property type="entry name" value="PNP_phzG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000190">
        <property type="entry name" value="Pyd_amn-ph_oxd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00558">
        <property type="entry name" value="pdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01064">
        <property type="entry name" value="PYRIDOX_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000167702" description="Pyridoxine/pyridoxamine 5'-phosphate oxidase">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="44"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="59"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="123"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="174"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01629"/>
        </source>
    </evidence>
    <sequence length="197" mass="22868" checksum="9208E56F48DDCB8B" modified="2005-09-27" version="1">MIKRDPIKLFDLWYDEVLAFPFKGKDPTAMVLATCNKDLKPSARVVLLKKHSDQGFIFFTNINSRKGKEITDNPFAALVFDWSYIAKQVRIEGKVKILPCQDADEYYASRPVGSKIGAWCSKQSSVLEDRMSFLKSIEKMTAEFHGKSIPRPDYWVGVVVVPMLMEFWQEGLNRIHTRYQYTRSNNMDVWNVVELYP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSS4</accession>
    <name>ARLY_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name type="ordered locus">Ecaj_0180</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304309.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSS4"/>
    <dbReference type="SMR" id="Q3YSS4"/>
    <dbReference type="STRING" id="269484.Ecaj_0180"/>
    <dbReference type="PRIDE" id="Q3YSS4"/>
    <dbReference type="EnsemblBacteria" id="AAZ68231">
        <property type="protein sequence ID" value="AAZ68231"/>
        <property type="gene ID" value="Ecaj_0180"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0180"/>
    <dbReference type="eggNOG" id="COG0165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_3_5"/>
    <dbReference type="OMA" id="KKNPDVF"/>
    <dbReference type="OrthoDB" id="751464at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000240727" description="Argininosuccinate lyase">
        <location>
            <begin position="1"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <sequence length="471" mass="53254" checksum="86243E51CA7453C4" modified="2005-09-27" version="1">MKNPLWGGRFTTSSSDIMKKINESISFDKTLYEEDIAGSIAHCKMLVNQRIISKYEGQLIIHGLEVIQNQISSGTFEFSTDLEDIHMNIEHNLKKMVGNIAGKLHTARSRNDQVATDFKLWIRKSIVKIEHYLHELQDTIINLAESHYNTIMPGFTHLQIAQPVTLGHHLMAYFEMFKRDFSRWQDLYKRMNQCPLGSAALAGTSFPIDRHFVAQELGFYSPTENSIDAVSDRDYAIEFLSNASICIMHLSRLAEEIILWCSYNFKFITLSDNITTGSSIMPQKKNPDAAELIRGKTGRIFASLNQILVVMKGLPLAYSKDMQEDKEALFDATNNLMLCIEAMNNMLSNIIINKNNMLKAAEHDYSTATDLADWLVKNLNLSFREAHETTGQIVKLAEHNQCKLHELTLEQIKTIIPSINDTVFSVLSVENSVASRTSYGGTAPTNVIEAIKKGKAYLSNIKFSQTDKNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSS6</accession>
    <name>Q3YSS6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dihydroorotate dehydrogenase (quinone)</fullName>
            <ecNumber evidence="7">1.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">DHOdehase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Dihydroorotate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Ecaj_0178</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-dihydroorotate + a quinone = a quinol + orotate</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0178"/>
    <dbReference type="EnsemblBacteria" id="AAZ68229">
        <property type="protein sequence ID" value="AAZ68229"/>
        <property type="gene ID" value="Ecaj_0178"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0178"/>
    <dbReference type="eggNOG" id="COG0167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013640_0_0_5"/>
    <dbReference type="OMA" id="ERIKMGA"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01036">
        <property type="entry name" value="pyrD_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0288">FMN</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHO_dh" evidence="16">
        <location>
            <begin position="46"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00018366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAZ68229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="350" mass="38658" checksum="7783EF790FC16DD9" modified="2005-09-27" version="1">MSFKQIFTNPLFCIPPEIAHKITIFALKNNFIPVKKVDIPRSLNIRAFNKIIKSPIGVAAGFDKNAEVIQPLLANGFGFVEVGTITKYPQRGNKKPRIHRLINKEALINSLGFNNKGVDFLIKKVDNIELNHCIFGINIGFNKKSKNPIQDYFDLVQKVYGLSNYITLNISSPNTPGLHEFQKKDLLLELLTAISQVRKSADYAESIPIMLKISPDINDNKKQDIVDLALQYKISGLIISNTSLQHKTLLNTDINIRGGLSGKPLFDLSTQVLSEIYQVSQGKLLLIGCGGVSSGYHAYEKIKAGASLIQLYTAIVYNGFNIANKISLELAELLAADGFTKVSDAIGCNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YST0</accession>
    <name>Q3YST0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304303.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68225">
        <property type="protein sequence ID" value="AAZ68225"/>
        <property type="gene ID" value="Ecaj_0174"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0174"/>
    <dbReference type="HOGENOM" id="CLU_257792_0_0_5"/>
    <dbReference type="OMA" id="VVDQKMS"/>
    <dbReference type="OrthoDB" id="1159708at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="608"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1258"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1266"/>
            <end position="1280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1306" mass="146396" checksum="54583C6CC72B19C3" modified="2005-09-27" version="1">MLARFDTSNKKLSSVPESSTQDSENGNVSLRNDSVNQRTADQYFSVFNLQHDVLENLNNVLLSYYEETFSFISLVEKNESFFLKTICSSCITNFFYDLRFFIYNLNTLVNNPTLKLDIPDVVISKDTLCQSCATFVLHYENILLHRTSTTMPSWQTVVCDDSHTTIQTNAVGEQQNRSINDNMLFISEQMDFVLSITGQIHAILLRLQQGKASYKELLEKSTDLVVLYNKCNVELKINTLTMFLKHILSELIECRSIFNVMLLCNKDKQAELLDRELQICRNDSNLRLVRILILNRVLSRENCRTRLFGDRNIAHSTQRVFNPKEQINSDSVDMHLMSDVKHVKSEVSNSDVFACNSGNGGNFKKTTDQLIQGQSDDVHHYYELECGSEIYTAMSNVGKEWDQNVHSNSKCDDDPAMHDYYSIDHEASGLDGTTPTFVSVPKDMLSDTQSSSATKGDRILDYKLSYEESDKASEACAVISSVNETRDQGMHSGSEYNNGSAMHDYYNIRYKASELDGTIPIFVSGPEDMLSDTQSSSATKRDRVLDYNLSYEESGKSSEACAVMASVDKTCDQSICRSGEYENNPAVCGYSIDHEASGLDGTTPAFVSGPKDMLGDTRSNSTTKRDGIPDRSDVNHSIVEYMKSEEGRRYLLDTLIREAGDMNQLVKILAAELGKEALVDILPNMSLAYIECVLSNDNTSVTHRRVDGNFVRPCGGGAFLKMQLLQDFISCVNVESNNKIEESASSKELFDKKSDRCVSVVSKDKLKIGSAVSRSYSDAKLVKKERYVRRKLSCRNNNAEDNVNLSNTLSSQIKEFVSFGTLDCMDVSNPSSSKQDLSSQASSCQSNKRDVSAENLPVVSGCMVDPVALVDQYAVHTNKVLENFIKDMIDGLNKSIDQYLRFTDKLQKESNLLNWVLYFLINKNSFSACKSAILEDLKPCFLYYNLITSIPDLDLHVKKLDASWVDSYVKSLHTLVHKSQINGVSFGVSKDYVIRRLSKYQMQVYDVKRLSQSFEVFNSISFSKQNLDSFVIKNKVIISLKYFKTNEICSELDGARYGGMIIKGDIPKKLILICNNLTLYGNVKGKVISSYGELIKIVGSVTGGLLINEESASDSSTVSVDNYVQDRSAIEVEDSIEYRNVPCMVEICGNVAGTVIVKNSDIIVNGKVSNKGSVISKFGKCACFVNTIAGSIMLKRTKYAIFNDVVYSTTHLIVEECDVYLRKEKKLQLDLGFIDSKFIPLSEGFKLDVSRLKKMIDEKNKRNSNDDGATVSTKPQRSKGNIVRSKNIHSFFSNVRVNSMSELMAI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-02-21" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YST5</accession>
    <name>RPOB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit beta</fullName>
            <shortName evidence="1">RNAP subunit beta</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoB</name>
        <name type="ordered locus">Ecaj_0169</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304298.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YST5"/>
    <dbReference type="SMR" id="Q3YST5"/>
    <dbReference type="STRING" id="269484.Ecaj_0169"/>
    <dbReference type="PRIDE" id="Q3YST5"/>
    <dbReference type="EnsemblBacteria" id="AAZ68220">
        <property type="protein sequence ID" value="AAZ68220"/>
        <property type="gene ID" value="Ecaj_0169"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0169"/>
    <dbReference type="eggNOG" id="COG0085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_4_1_5"/>
    <dbReference type="OMA" id="FMTWEGY"/>
    <dbReference type="OrthoDB" id="9601at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01321">
        <property type="entry name" value="RNApol_bact_RpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042107">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_ext_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019462">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_external_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010243">
        <property type="entry name" value="RNA_pol_bsu_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10385">
        <property type="entry name" value="RNA_pol_Rpb2_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02013">
        <property type="entry name" value="rpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000224054" description="DNA-directed RNA polymerase subunit beta">
        <location>
            <begin position="1"/>
            <end position="1380"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01321"/>
        </source>
    </evidence>
    <sequence length="1380" mass="155495" checksum="A9F4B74AE87C6319" modified="2005-09-27" version="1">MSSSVTPTKYVLNSFNSVPRLSYAKSIDIKDSLTDLIKIQRDSYNAFIGVGQNTESGIKNIFESMFPIEDLLGRAVLEFVSYSIGEPQYDEYECIKRGITFSVPIRIVLRFIVWKVQEVSFKEVKYVVDEETSEKSIKYIKEQEVSIGDLPTMTPHGTFIINGIERVIVSQMHRSPGVFFDSDKGKTYSSGKLIYSARIIPYRGSWLDFEFDIKDILYFRIDRKRKLPVSLLLRALGLSNNDILNTFYDKIRYVKCEKGWIVPFVVDRFRGVRLSHDLVDVNGNVLVKANTRITLRMAKKLANDGLTEYLVPFAEIQGLFIANDLFDPSGNALIISAGENITSEHINKLELFDIKEIFVLNIDFLTVGPYILNTLFLDKNVTYEDALFEIYKVLRSGESPSLDTIKAFFDGLFFEKERYDLSTVGRIKLNDHLGLNVSEDITVLTKDDIIHVIKKLVLLRDGEGSVDDIDHLGNRRVRSVGEFIENQFRIGILRLERMIMDYMSSVNFDNAMPCDFVNPKILATVLKDFFSSSQLSQFMDQTNPLSEVTHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRICPIETPEGQNIGLISSLAIYARINKHGFIESPYRKVDKGVVTDKVEYLLAMQESNYYIADASATLDENNQFVDDMLYCRHDGNFVMVKREEVDYIDVSPKQIVSVAASLIPFLENNDANRALMGSNMQRQAVPLLKADAPLIGTGMESIVAAGSGTVVLAKRGGIVHRVDGLYIVIRAFDQEKNEYLGVDVYNLRKFQRSNHNTCINQRPLVKPGDYVKANDVIADGSAIDQGELALGKNVLVAFMSWQGYNFEDSIVISSEVVKKDVFTSIHIEEFECVVRDTTLGPEKIMRSVPDINEDSLSHLDDVGIVNIGAEVSAGDILVGKVTPRPPISLPPETKLLVTIFGEKVFDCVDSSLYLPLDVEGTVVDVHVFVRRGVEENDRSLLIKQNEINGFIKERDYEIDVVSEYFYDELKRVLVNSGVQCNNQNVNDYLESTPKKDWWNVNLSDETVLLQINNLREKFDSMIQNAHSKFDQKIDKLNYGYDLPQGVLCIVKVFVAVKHNLQPGDKMSGRHGNKGVISRIVPVEDMPYLEDGTPVDIILNSLGVPSRMNVGQILETHFGWASVNLGKKIGHILDNIDELTISHLRNFLDQVYDGQDLKYNIQSMSDEDLLAFAERLRGGVPMAAPVFEGPKDSQISNLLKLADLDVSGQVDLYDGRIGEKFDRKVTVGYIYMLKLHHLVDDKIHARSVGPYGLVTQQPLGGKSHFGGQRFGEMECWALQAYGAAYTLQEMLTVKSDDIVGRVKIYESIIKGDSNFECGIPESFNVMVKELRSLCLDVALKQDKDFLSSEVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-04-07" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSU0</accession>
    <name>Q3YSU0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Transcription termination/antitermination protein NusG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nusG</name>
        <name evidence="8" type="ordered locus">Ecaj_0164</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Participates in transcription elongation, termination and antitermination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the NusG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304293.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0164"/>
    <dbReference type="EnsemblBacteria" id="AAZ68215">
        <property type="protein sequence ID" value="AAZ68215"/>
        <property type="gene ID" value="Ecaj_0164"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0164"/>
    <dbReference type="eggNOG" id="COG0250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067287_1_0_5"/>
    <dbReference type="OMA" id="DGPFNGF"/>
    <dbReference type="OrthoDB" id="2027986at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00948">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043425">
        <property type="entry name" value="NusG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001062">
        <property type="entry name" value="Transcrpt_antiterm_NusG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265">
        <property type="entry name" value="PTHR30265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265:SF2">
        <property type="entry name" value="PTHR30265:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02357">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00338">
        <property type="entry name" value="NUSGTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82679">
        <property type="entry name" value="SSF82679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00922">
        <property type="entry name" value="nusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5 6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="3 5 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 5 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="NGN" evidence="7">
        <location>
            <begin position="2"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="181" mass="20817" checksum="E7917D87483B0D2A" modified="2005-09-27" version="1">MKYEWYIIQVSSGSEEKVCQAILENSCVLGLGENFREVFIPYEEVTRVKHNKKILVKRKLSPGYVFLYMNLNENSINFVKSIPRVLNFLNNDFGVPKVISDSEMESMRKKMCQSVVDDTNVVSFEIGDEVVINDGLFQDFNGKVEYINDDKKIAGVSVMIFGRLTKIEFKLEHIQKVEAQS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSU3</accession>
    <name>EFG_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor G</fullName>
            <shortName evidence="1">EF-G</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fusA</name>
        <name type="ordered locus">Ecaj_0161</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSU3"/>
    <dbReference type="SMR" id="Q3YSU3"/>
    <dbReference type="STRING" id="269484.Ecaj_0161"/>
    <dbReference type="EnsemblBacteria" id="AAZ68212">
        <property type="protein sequence ID" value="AAZ68212"/>
        <property type="gene ID" value="Ecaj_0161"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0161"/>
    <dbReference type="eggNOG" id="COG0480">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_4_1_5"/>
    <dbReference type="OMA" id="AATTCHW"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225211" description="Elongation factor G">
        <location>
            <begin position="1"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="8"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <sequence length="690" mass="76169" checksum="D1331B1D9CDBD424" modified="2005-09-27" version="1">MNIDNELSKCRNIGIMAHIDAGKTTTTERILFYTGKQNRIGEVHEGAASMDWMEQEKERGITITSAATTCFWNGHRINIIDTPGHVDFTIEVERSLRVLDGAVAVFDGVAGVEPQSETVWRQADKYNVPRICFMNKMDRMGADFYRCVDMLVDRLGATPLVLQLPIGSEKDFVGVVDLLEMRSIIWDEDSLGASFHYGEIPKDMIDKAQEYRNKLLESAVELNDEAMNLYFEGKEISVSLLKSCIRTGVIQSKFVPVLCGSAFKNRGVQPLLDAVVDFLPAPNDIPVIEALDVKTSNTINVKTSAGGKFVALAFKVMTDKFVGSLTFIRIYSGKLSSKTTVLNAVKDSTESIGRILLMHANNREDITEAQAGDIVALAGLKKTVTGDTLCALDYPVILERMEFPEPVMEIAVEPKSTADQEKMGIALSRLVAEDPSLGMYVNPESGQTILKGMGELHLEVIVDRMRREFNVEANIGAPQVAYRETITKSVEIEYIHKKQTGGAGQFAKVNILFEPLPPGSGFQFESKITGGAIPKEYIPGVQSGLENIRGSGMLAGFPVIDFKATLFDGAFHEVDSSPLAFELAAKGAFRDMVNKAGAILLEPIMKVEIITPDEYMGDVIGDINSRRGRVAEMQDRHNTKVILAFIPLAKMFGYVKDLRSMSQGRAQYSMYFSCYEQVPDNIVANEIKTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-24" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSU5</accession>
    <name>RS12_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">30S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpsL</name>
        <name type="ordered locus">Ecaj_0159</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">With S4 and S5 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304288.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSU5"/>
    <dbReference type="SMR" id="Q3YSU5"/>
    <dbReference type="STRING" id="269484.Ecaj_0159"/>
    <dbReference type="EnsemblBacteria" id="AAZ68210">
        <property type="protein sequence ID" value="AAZ68210"/>
        <property type="gene ID" value="Ecaj_0159"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0159"/>
    <dbReference type="eggNOG" id="COG0048">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104295_1_2_5"/>
    <dbReference type="OMA" id="SPALEKC"/>
    <dbReference type="OrthoDB" id="1707228at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_B">
        <property type="entry name" value="Ribosomal_S12_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_S12/S23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_S12_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="PTHR11652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000295972" description="30S ribosomal protein S12">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="modified residue" description="3-methylthioaspartic acid" evidence="1">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="123" mass="13658" checksum="B1F78A2AC97E7B4F" modified="2005-09-27" version="1">MPTINQLVRKPRKSRSALNKAPALQHNPQKRAVCVKVYTTTPKKPNSALRKVARVKIAGYGSEVIAYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGALDSRGVQNRKKARSKYGVKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSU8</accession>
    <name>Q3YSU8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COQ9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0156</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304285.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0156"/>
    <dbReference type="EnsemblBacteria" id="AAZ68207">
        <property type="protein sequence ID" value="AAZ68207"/>
        <property type="gene ID" value="Ecaj_0156"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0156"/>
    <dbReference type="eggNOG" id="COG5590">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057411_3_0_5"/>
    <dbReference type="OMA" id="LANSFCK"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013718">
        <property type="entry name" value="COQ9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012762">
        <property type="entry name" value="Ubiq_biosynth_COQ9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427">
        <property type="entry name" value="PTHR21427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08511">
        <property type="entry name" value="COQ9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02396">
        <property type="entry name" value="diverge_rpsU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="COQ9" evidence="3">
        <location>
            <begin position="108"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="205" mass="24196" checksum="CA4F674F1660CA61" modified="2005-09-27" version="1">MNNQSSIIKATIALIPFYGLSNDTLLKACTDLDLDEEFYKFHNGIYDVLNSINDDLINFITTKFYETDNVNNFKIREKIQHAVHLCLIYYTSLPNYRELLKHILSAAPYNMCFAAKFLYKVVDYIWDLIGDRSTDFNYYTKRSTLAIVYASTVMYFINDFSDNHSDTILFLERRINNVIQIHKLKSYIFNKTEKFNIFKVKFDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSV1</accession>
    <name>Q3YSV1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nickel-dependent hydrogenase b-type cytochrome subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0153</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304282.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0153"/>
    <dbReference type="EnsemblBacteria" id="AAZ68204">
        <property type="protein sequence ID" value="AAZ68204"/>
        <property type="gene ID" value="Ecaj_0153"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0153"/>
    <dbReference type="eggNOG" id="COG3038">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095321_4_0_5"/>
    <dbReference type="OMA" id="WYKTAPH"/>
    <dbReference type="OrthoDB" id="1940783at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011577">
        <property type="entry name" value="Cyt_b561_bac/Ni-Hgenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01292">
        <property type="entry name" value="Ni_hydr_CYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="SSF81342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Ni_hydr_CYTB" evidence="3">
        <location>
            <begin position="4"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="173" mass="19665" checksum="60C5D8F44EE5EB1F" modified="2005-09-27" version="1">MTKYHSLVRIMHWLTGIPIILMVIMGFCLKMDVFPSNYPIGELYTLHKAIGIILLSLLILRLVCRFSSIIPPYPNSFPHYLILVSKITHLGLYITSITMAMSGYVMSSASGRAIKIFSFDVPLLIENNKNIANIAQQCHNICAYMLSILIIMHILAALKHKFIDKDNIFNRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSV2</accession>
    <name>Q3YSV2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-nitropropane dioxygenase, NPD</fullName>
            <ecNumber evidence="2">1.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.1.9" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304281.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0152"/>
    <dbReference type="EnsemblBacteria" id="AAZ68203">
        <property type="protein sequence ID" value="AAZ68203"/>
        <property type="gene ID" value="Ecaj_0152"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0152"/>
    <dbReference type="eggNOG" id="COG2070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038732_1_1_5"/>
    <dbReference type="OMA" id="IDDLPSC"/>
    <dbReference type="OrthoDB" id="275153at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016631">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMO" evidence="1">
        <location>
            <begin position="11"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="345" mass="37536" checksum="EFA2F82DE86968D6" modified="2005-09-27" version="1">MLSELWKIGIEFLGSNFAIMGGAMSWVSDPYLVSAISNAGGFGVLACGSIDPDELRKEIELTQQLTNNPFGVNLIIMHPSLNELIEVCIEKRVTHIVLAGGIPNNKTITHIKDAKIKIMCFASSLSIGKRLVRTGVDALIIEGMEAGGHIGPVSTSVLAQEILPYFREEESLKKIPIFIAGGIGRGEMMAHYLIMGASGCQIGTLFVCTHESRAHQNFKEIFIKSSSRDAMSSSQLSKDFPVIPVRAIKNDASVKFLELQKEIINLYHQGKISKEAGQLSIEKFWAGALRKAVIDGDITNGSLMAGQSVGMVREIQTTEQVINGLISQACNYITSVQKINTIQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSV4</accession>
    <name>Q3YSV4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphate ABC transporter substrate-binding protein, PhoT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304279.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0150"/>
    <dbReference type="EnsemblBacteria" id="AAZ68201">
        <property type="protein sequence ID" value="AAZ68201"/>
        <property type="gene ID" value="Ecaj_0150"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0150"/>
    <dbReference type="eggNOG" id="COG0226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026228_0_0_5"/>
    <dbReference type="OMA" id="GTKHGTR"/>
    <dbReference type="OrthoDB" id="1222928at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004231035" evidence="1">
        <location>
            <begin position="21"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="2">
        <location>
            <begin position="20"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="340" mass="38468" checksum="BD7538A9E213DDDA" modified="2005-09-27" version="1">MSRFFLVFLVLITLAFNVNAQQIRVVGSSTASPFISAIAEEFGRFSDYGTPIIESVGSGMGFSMFCEGVGKNTPDIVMSSRKIKDAEVKLCKSNNIENIIEIIIGYDGIVIANSKDSNKLDFTKQDLFKALSKYSTSNEYVETMSTNYFRYWSEINNRLPNTDIEVYGPYKNTGTYNILVEEIMQASCIHNQNFIDVYPDLKRRQRACGIMRNDGKYIEVAANENIIIQKIAKNHDAFGILSFSFLIKNQDKIHANKIAGVEPNYETISSGKYILSRPIYLYIKQEHINLSSSIKEFIKVILREESIGKNGYLIGLGFIPLSDKDLLDTKNRITNILDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSW7</accession>
    <name>Q3YSW7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription termination factor Rho</fullName>
            <ecNumber evidence="8">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP-dependent helicase Rho</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rho</name>
        <name evidence="11" type="ordered locus">Ecaj_0137</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. The homohexamer assembles into an open ring structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304266.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0137"/>
    <dbReference type="EnsemblBacteria" id="AAZ68188">
        <property type="protein sequence ID" value="AAZ68188"/>
        <property type="gene ID" value="Ecaj_0137"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0137"/>
    <dbReference type="eggNOG" id="COG1158">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016377_4_3_5"/>
    <dbReference type="OMA" id="TRLCRAH"/>
    <dbReference type="OrthoDB" id="1619125at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008186">
        <property type="term" value="F:ATP-dependent activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04459">
        <property type="entry name" value="Rho_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01128">
        <property type="entry name" value="rho_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01884">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041703">
        <property type="entry name" value="Rho_factor_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011112">
        <property type="entry name" value="Rho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036269">
        <property type="entry name" value="Rho_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011113">
        <property type="entry name" value="Rho_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004665">
        <property type="entry name" value="Term_rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46425">
        <property type="entry name" value="PTHR46425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07498">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07497">
        <property type="entry name" value="Rho_RNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00959">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68912">
        <property type="entry name" value="SSF68912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00767">
        <property type="entry name" value="rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51856">
        <property type="entry name" value="RHO_RNA_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 8" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="Rho RNA-BD" evidence="10">
        <location>
            <begin position="103"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="227"/>
            <end position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="239"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="476" mass="52957" checksum="243A3621908C3417" modified="2005-09-27" version="1">MLNEKKKRTRYVSRKNANKNISIQSTANIVDKSEEVGDKEEVEGSAQVEAQEVKVLDLSELKKKSIEDLLKIAEELGVVSNGRMLKQEIIFHLMKKVVNDGGAAIGGGVVEILSDGFGFLRSPEANYAASGDDVYISAGQIKKFNLRTGDIVSGEIRAPSEKERYFTLVKAHSINFTDMGKLQRYVHFDDLIPLYPEERILLETNDPVSLSKKDISMRVIDIVAPLGKGQRALIVAPPRAGKTIILQQIAHSISVNHPDIELIVLLIGERPEEVTDMCRSVKGEVVSSTFDEPGYRHVQLAEIVIEKAKRMVEHKKNVVILLDSITRLARAYNSVIPSSGKVLTGGVDSNALQRPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNCEIILDRKISDKRVYPAIDISKSGTRKEDMLIDHVCLKKVWLLRRLLSSMGSVEAMEFLRDKLSITKDNNEFFDMMNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSX6</accession>
    <name>Q3YSX6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Extracellular solute-binding protein, family 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0128</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304257.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YSX6"/>
    <dbReference type="STRING" id="269484.Ecaj_0128"/>
    <dbReference type="EnsemblBacteria" id="AAZ68179">
        <property type="protein sequence ID" value="AAZ68179"/>
        <property type="gene ID" value="Ecaj_0128"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0128"/>
    <dbReference type="eggNOG" id="COG1840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026974_2_1_5"/>
    <dbReference type="OMA" id="HPYNVSL"/>
    <dbReference type="OrthoDB" id="1567509at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055072">
        <property type="term" value="P:iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026045">
        <property type="entry name" value="Ferric-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002825">
        <property type="entry name" value="CfbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0574">Periplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR002825-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="347" mass="38819" checksum="C57F2A3558530F8A" modified="2005-09-27" version="1">MMRLLACLGIVAVIIVAFNFLTNKQQVQDQKQEVHVYSSRKEELLHSLFEQFTKETGIKVKYINDEAAQLINRMENEGTATSADVFLTADAVNLILAKKKGLLQPIQSEVLNQAIPSKYRDSEGFWFGLTKRARVIVYNKDLVEDNELSTYEHLANTKWKDKILVRSSSSPYNQSLIAFMVANNGVENTKIWVKGLVANMARKPSGGDIDQIYAVAADEGSIAIVNSYYFGRIAASDKKSDQAVVKKLGIFFPNQETTGTMINISGGAVTKHAKNKQNAIKLLEFLTSVRAQKVYAQVNQEYPIVEGVELSEVLKTFGTFKESDLPLQELEKHLTKSVEIADECGWR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSX9</accession>
    <name>NRDR_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcriptional repressor NrdR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nrdR</name>
        <name type="ordered locus">Ecaj_0125</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NrdR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304254.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSX9"/>
    <dbReference type="SMR" id="Q3YSX9"/>
    <dbReference type="STRING" id="269484.Ecaj_0125"/>
    <dbReference type="EnsemblBacteria" id="AAZ68176">
        <property type="protein sequence ID" value="AAZ68176"/>
        <property type="gene ID" value="Ecaj_0125"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0125"/>
    <dbReference type="eggNOG" id="COG1327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108412_0_1_5"/>
    <dbReference type="OMA" id="YRFTTYE"/>
    <dbReference type="OrthoDB" id="1551627at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00440">
        <property type="entry name" value="NrdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003796">
        <property type="entry name" value="RNR_NrdR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30455">
        <property type="entry name" value="PTHR30455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00244">
        <property type="entry name" value="TIGR00244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000230867" description="Transcriptional repressor NrdR">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-cone" evidence="1">
        <location>
            <begin position="49"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="zinc finger region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00440"/>
        </source>
    </evidence>
    <sequence length="153" mass="17734" checksum="042640F60FF8EC55" modified="2005-09-27" version="1">MKCPFCNNINTQVKDSRAIEDDILIRRRRICLVCNSRFTTIEKLLLRSFMVIKKNGETEPFNKQKLLSSILIATKKRPVSHEGINVMVNNIFYELEGKKENAVPTDVIGKMVMDNLFKLDKVAYVRFASVYMNFKNINDFSNIIAKIINEQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSY2</accession>
    <name>Q3YSY2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304251.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0122"/>
    <dbReference type="EnsemblBacteria" id="AAZ68173">
        <property type="protein sequence ID" value="AAZ68173"/>
        <property type="gene ID" value="Ecaj_0122"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0122"/>
    <dbReference type="HOGENOM" id="CLU_2287062_0_0_5"/>
    <dbReference type="OMA" id="QENIGSM"/>
    <dbReference type="OrthoDB" id="2084064at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="103" mass="11807" checksum="4742C0F626F549F9" modified="2005-09-27" version="1">MVMSYFHYILFYITDNPKWYILLAVILLIGTGYFLRQKAAKKSKKDSSIASYYANNMNGSSPAKPHNKNYIPELNTAQNKQQSSFIVESVSQVFESKTQGRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSY5</accession>
    <name>Q3YSY5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AsmA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304248.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68170">
        <property type="protein sequence ID" value="AAZ68170"/>
        <property type="gene ID" value="Ecaj_0119"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0119"/>
    <dbReference type="eggNOG" id="COG2982">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_327536_0_0_5"/>
    <dbReference type="OMA" id="YLSTSWN"/>
    <dbReference type="OrthoDB" id="109242at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007844">
        <property type="entry name" value="AsmA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05170">
        <property type="entry name" value="AsmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="AsmA" evidence="2">
        <location>
            <begin position="602"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="881" mass="96240" checksum="630216C20BE4D6BE" modified="2005-09-27" version="1">MRILIYSGGIILTLLLFLIISPFFINWNSYYASHILKQIEDISDYVTVKGVGSVTGKLILPKIIVNNLYIEGHGKLSTHKSTILVNQLELKISLLSLLLFSPKVNTITVDGLSIPLGNLVSLFSSFQGKSFNINTFNIVNSVVNTDYDGTSFNSKPISIKEGSIKTINGAKVINGLLSIGKNDYNLVGDVSVNEDHNKINANVYSQSTKVTLSGVASGNKFDGVVLANGTDFSQFINDISETNKTSIFSFINSNEEFSLSANLKLSGQSFELNDLKVSTDSIDGIGKIVCSSYASCDASVDFSKINIDHLSGSNNGSDAYSKESRTLDYFNTLISENLDYKVKISAKEIKYHDEVSSNLALDFEVLKGKININKMTVMLPGNNNVLSIEGSIGSSDLISSFNGKLKVVGDDFVSFAKWLFPIQINLESSSKFSIQSDLYLAPRVFSLSNVEILTKNFGDAEGQLKIKYDKKSRSVSGSIGLSNIDFDLYKVNEELNLENFMPMKWLKDIKYKINVETRLSNFLIQKQRVDNLSFLVSIVQGKFGIDKIRFSGADGSDLSGFVKASIGSQDLKPKISVNLKGNKFSTAFIKFPALIHDVVDASNKVTNIKWLNHDLKFYGLEHVDGDIDINIKNFVAKNNALTDFVFSSSLKDSLMSINKLMFKIDDGFVSTSGKVGIGSNTSSLSAVISVANIGLNNLLQYARVDGVTGNVSISGSIQTQGKSLVDWVNALEGKMEIVAKGVNVDGVDFNKFIIDLLDAKSKSDVVALTQIGLYDNNTVFNFINAGANISRGNVISSLQFAIDNASGVASANVSLLQFAVTSKVRLSFMRPGVSLPANIDMSLYGQLWQPKMSFNVNSLYEEIVKGNSGYVSTQDEEEFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSY6</accession>
    <name>Q3YSY6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Allosteric NADP-dependent malic enzyme</fullName>
            <ecNumber evidence="11">1.1.1.40</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ecaj_0118</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.40" evidence="11"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304247.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0118"/>
    <dbReference type="EnsemblBacteria" id="AAZ68169">
        <property type="protein sequence ID" value="AAZ68169"/>
        <property type="gene ID" value="Ecaj_0118"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0118"/>
    <dbReference type="eggNOG" id="COG0280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012366_2_0_5"/>
    <dbReference type="OMA" id="INNVMGF"/>
    <dbReference type="OrthoDB" id="654531at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012188">
        <property type="entry name" value="ME_PTA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042113">
        <property type="entry name" value="P_AcTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042112">
        <property type="entry name" value="P_AcTrfase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036684">
        <property type="entry name" value="ME_PTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="4 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="malic" evidence="10">
        <location>
            <begin position="26"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Malic_M" evidence="9">
        <location>
            <begin position="171"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="84"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR036684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036684-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036684-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="763" mass="83327" checksum="8C7A75B1679233DC" modified="2005-09-27" version="1">MLDLENSSFDGKKKEALEFHCKDSKPGKVSLLPTKPLLTQRDLSLAYSPGVAVPCLEIAKNPDLIYEYTARGNYVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKRFADIDAVDIEVDTSDVDEFINAVKYLGLSWGGINLEDIKAPECFIIEKKLSEIMDIPVFHDDQHGTAIIVSAGLINALDITGKNFKNVKIVINGAGAAGIACLEMIKLIGVPADNITLCDQNGVIYKGRQLGMNEWKEKHAIETKDRSLKDALVMADVFLGLSVKDVLSKDMLLSMNKDPVIFALANPDPEINPDIAREVRPDAIIATGRSDYNNQINNVMGFPYIFRGALDVRAKSVNNEMKVAAANAIAMLAREYVSDEVSDAYGGRKMNYGKDYIIPTPFDPRLITIVSPAVARAAIESGVARKKIEDWDEYTKQLASRLSSTSNILNMMYSAVKRDPKRVIFSEGEEEKVIKAAVQWRNQGYGLPILVGRIDKVQEAFERLGIKNIQGIEIANAAVSKRNDEYTDYLYKKLQREGYLYRSCVRDVKTDRNVFAACMLACGDGDVLITGLTRSYSASISDVQKVIGSQGVVFGLSVIVMKERTIFVADTAIHESPTPEQIAQIAIQASIQARKMGHEPRVGFVSSSNFGSHCQEDARKMRKAVEILDSCNVDFEYDGEMSVDTALSSELLTLYPFCRLTGAANILVMPTLHSANISSKLLQQAVGVSVIGPVLIGMEKPVQIVQMSSSVSEILNLAVLAPSVNSNVQSCDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT00</accession>
    <name>Q3YT00_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304233.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68155">
        <property type="protein sequence ID" value="AAZ68155"/>
        <property type="gene ID" value="Ecaj_0104"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0104"/>
    <dbReference type="HOGENOM" id="CLU_645186_0_0_5"/>
    <dbReference type="OMA" id="NICCIVI"/>
    <dbReference type="OrthoDB" id="2110086at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="425" mass="47811" checksum="3DC1A27007046908" modified="2005-09-27" version="1">MLGKDYFSDLDKRLVAFAKLNNGKQRSSKYHFMLSSCILITAIITVLSLIVLYSDKQYFSSMFKGGFKLFSSDTIPFSMALIAIIPSLLLLFFLIYKVCAFHDLNRKLNNESIDILGKLEEWQYFLHLQLEYNTNKIENIDEVLKLFKKKYSSFVKQCSDGFAKLEEQCKSVLAAVNSTTHGVNAGISKINCVVEDVKLQLNGLSAGCQKFSESSSNLLSAIESAIKTEGVNVDDRIAFLQNLQANLASDVDLGLIQNRVQNFVARVRSILVEKPSIVKPAVLRELWCLQESFNVIIMKVHKTDQCALEFLQLIQNLEQEISKFSLSASKKIASYNGNNRNLLEKMIKLELPLLHINILRFLTAKRAAFITNTSADITQFIQEYASGDLQRLSDSTSDSMLSISSFLSLQDTNVSDKVSNDINPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT04</accession>
    <name>Q3YT04_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0100</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0100"/>
    <dbReference type="EnsemblBacteria" id="AAZ68151">
        <property type="protein sequence ID" value="AAZ68151"/>
        <property type="gene ID" value="Ecaj_0100"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0100"/>
    <dbReference type="eggNOG" id="COG0312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_1_0_5"/>
    <dbReference type="OMA" id="TDFWGSM"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD_M" evidence="3">
        <location>
            <begin position="124"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="2">
        <location>
            <begin position="241"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="477" mass="51369" checksum="BCE3A089CD514329" modified="2005-09-27" version="1">MVMNISNVEQMLFTRNQINENTVNRVVSDCLCAADGGELFLEMCISEALGFEDGILKYADFNSRQGFGLRSFCKELTAFVCSSEITEQEISNAAALVKSINSSGNGGVIHLNKTKPALYPETNPLQSITFDIKVKLLQEVESYLHTKSPYVKQVKTALSGQWQMVHIILPDGNKVYDIRPLVRFNVCVILEKDGRKESGSAGHGGRASYEEFIGDGKWQVIADEALRQALVNLESIPTPAGEMTVVLGPGWPGVLLHEAVGHGLEGDFNRKKVSAFSNSIGKKVAADGVTVVDDGTISGRRGSINVDDEGVPSSYNVLIQDGILVNYMQDRMNANLMGTTSTGNGRRQSYQDVVIPRMTNTYMLPGNYHPEEIISSVKKGIYAVHFSGGQVDITSGKFVFSASEGYLIEDGKVTHPVKGATIIGNGPDVLTKVSMIGNDLALDSGIGTCSKNGQDLPVGVGQPTLKIDNIVVGGTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT06</accession>
    <name>Q3YT06_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="3 9">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ecaj_0098</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="function">
        <text evidence="9">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>(R)-N(6)-lipoyl-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + H(+) + pyruvate = (R)-N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10480"/>
            <dbReference type="Rhea" id="RHEA-COMP:10481"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="3 9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304227.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0098"/>
    <dbReference type="EnsemblBacteria" id="AAZ68149">
        <property type="protein sequence ID" value="AAZ68149"/>
        <property type="gene ID" value="Ecaj_0098"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0098"/>
    <dbReference type="eggNOG" id="COG0022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012907_1_1_5"/>
    <dbReference type="OMA" id="SRMRHHC"/>
    <dbReference type="OrthoDB" id="900125at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:acetyl-CoA biosynthetic process from pyruvate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="PTHR11624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transket_pyr" evidence="10">
        <location>
            <begin position="4"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="332" mass="36247" checksum="93DF4A82FB569E17" modified="2005-09-27" version="1">MKSLTVREALCAAIREEMERDHTVLIMGEEVGEYQGAYKVTQELLAQFGPERVIDTPITEHGFAGIGVGAAFGGLKPIVEFMTFNFAMQAIDQIINSAAKTNYMSGGQLNCPIVFRGPNGAAARVGAQHSQCYASWYAHVPGLKVISPYFAADCKGLLKAAIRDPNPVIFLENEIAYGHKHEIEDEVLTSDYTTEIGKAAIVKEGMDITITAFSIQVKNALAAAELLEKEGINAEVIDLRTLRPLDTETILCSIKKTNRIITVEEGWPYSGIGSEIAALIMEQAFDDLDAPVIRVTGKDVPLPYAANLEKLSLPQVTDILEAARILCLRNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT08</accession>
    <name>Q3YT08_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304225.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0096"/>
    <dbReference type="EnsemblBacteria" id="AAZ68147">
        <property type="protein sequence ID" value="AAZ68147"/>
        <property type="gene ID" value="Ecaj_0096"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0096"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076204_1_0_5"/>
    <dbReference type="OMA" id="HCPYVKH"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="9"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="194" mass="22123" checksum="EBA3D436901F4DB3" modified="2005-09-27" version="1">MVALYTPQVDKNVIAHDFSLKSTDGNLYSLSSYHKGNGLLIMFICNHCPYVKAIINNLVYDVNTLKNDYNIYTIAIMPNDTIAYPDDSYENMIKFAQEYGINFPYLIDEDQTVAKNYGAVCTPDFFGFNNKLELCYRGRFDSSGKNQVDSKQEDRDLFNAMKFISKTGKAPDNQKPSIGCSIKWKSQDDQFVPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT17</accession>
    <name>Q3YT17_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cytochrome c assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0087</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304216.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0087"/>
    <dbReference type="EnsemblBacteria" id="AAZ68138">
        <property type="protein sequence ID" value="AAZ68138"/>
        <property type="gene ID" value="Ecaj_0087"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0087"/>
    <dbReference type="eggNOG" id="COG1138">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015041_3_0_5"/>
    <dbReference type="OMA" id="SYWGMQL"/>
    <dbReference type="OrthoDB" id="213184at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032523">
        <property type="entry name" value="CcmF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002541">
        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003567">
        <property type="entry name" value="Cyt_c_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003568">
        <property type="entry name" value="Cyt_c_biogenesis_CcmF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43653">
        <property type="entry name" value="PTHR43653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16327">
        <property type="entry name" value="CcmF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01410">
        <property type="entry name" value="CCBIOGENESIS"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01411">
        <property type="entry name" value="CCMFBIOGNSIS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrom_C_asm" evidence="8">
        <location>
            <begin position="80"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="CcmF_C" evidence="9">
        <location>
            <begin position="304"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="644" mass="73507" checksum="71F6F6221CB6FA35" modified="2005-09-27" version="1">MINLIELPLLIACVFSIVYPFVSYLSFCWSKVFTILIFMLTSSAMGLLLYTHIVDDFSFYNVYYNSHTTKPLLYKIFGIWGNHEGSILLWVWIISFYSLLLEFFLEREDLKKIAISIQHIMNFGFLLFTLFISNPFLKMPTIEYEGLGFNPILQDTGLMIHPPILYLGYVGFSVVFSLAVAGVIVQKDSVKWATIINKWVLLAWSFLTLGIGLGSWWAYRELGWGGFWFWDPVENASLMPWLFGTALLHLLPLVRKFNIFYNFAILLSIFSFIMSLIGTFLVRSGVLISVHAFANDPKCGFYILMLIGIIISSGIVSFICFAKKAQEKKHDFLLLSKVTMLLFNNVLLLTAFTIVFIGTIYPIVLEVITGDVISIGAPYYNSLFNYLIFCTLLLMIIVMGLDKKRNKILVENFKFSGLITSLIVPFIIDNNFFIIAMILLSVLLFLSVLETYCRKIRILAVPFCETARLIKSYSTKYYSMLVSHIGVAVFIFGVIGSTVWGETYELYMKEGNSVKTKNLQVVLSKLSFVKNENYNAIRGKFLIIKSGEILDKIFPENRFYIVEGVRNTESAIYRHWLSDVYIVIGDIDKAMGIAVKIYYKPFINLIWIGFLLITFGGVIGLFANTTTRKASMFEELKADDSTCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT35</accession>
    <name>Q3YT35_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304198.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68120">
        <property type="protein sequence ID" value="AAZ68120"/>
        <property type="gene ID" value="Ecaj_0069"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0069"/>
    <dbReference type="HOGENOM" id="CLU_396260_0_0_5"/>
    <dbReference type="OrthoDB" id="2106818at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021902">
        <property type="entry name" value="DUF3514"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12027">
        <property type="entry name" value="DUF3514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="823" mass="90171" checksum="7A9277444183A153" modified="2005-09-27" version="1">MLLILLTIVSFIGGAAASALLRLITSNTSQDSDKVSGKDEPGSNEESSQEFLKAGQDQAKAKAKAGGAVVVSVLQMQGGKKKKRSRHSKQIVQGTDKTSQQSAKDDADTVSDKDTKKGKDTREGSGKEVQSADSEKGVQCIGENGLDQLVQDGADTTSDKDVKKKASTQDPLKKDEQLKSDRKDVEGPNKTVQSQQEVNALPVKEKVGDIGVSVDHSSMQHANDQQISVNVGSEASQYLLLGARPKVRVGKTSKSKKSKEPAVKFYDWSSKNYRDKDPFLVQAVEESRKGRAISNVVTTQTSEKVLPAGDFQVEQKHVDSATDISVGLGVSQQPGSDKVVADESLLVEQETNIHKQCLSLKVVSTHEQDIVSIVEQVSLDELGAAVRIDPAASDQGIHLEESSLVMQEDSMLTTVEQVSLDNLGAAACVVPVETSDRSVQERKASVKETGNLSTHVKSVGISKVASANGKRTLHKNILSEFIKSSAKSAVGEDIEKNKADFKQRICVFKAIHFEYCRSIGRELFEEQRGKILLRKGIISFIKRVIPNRKICTFIFKLAYMLNCMEMVNRPDVANSMICNLLLYSNQSLLDENLVQIVQCVQSGANVEEASKVLHGCCFKMANCDHEFYDGEFFRNVLDVFQKLMLFRLVGANCPKKVLDLVIVKCAMIIGTMYTGYRCFIKSQGGFSDDESIAAIIEHMYGGGGDAKTKPHAGRVGVICCNMLVNLMNMYDPESNGIVQISGICFPEKLICQCSEHLSNEIERNMAEGTMGYEVIMENLAMCLYSTIENFDIAACREETEVYSAIYNEIIRSSGVSKGKWHNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT49</accession>
    <name>SYY_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="1">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tyrS</name>
        <name type="ordered locus">Ecaj_0055</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L-tyrosyl-tRNA(Tyr)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304184.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT49"/>
    <dbReference type="SMR" id="Q3YT49"/>
    <dbReference type="STRING" id="269484.Ecaj_0055"/>
    <dbReference type="EnsemblBacteria" id="AAZ68106">
        <property type="protein sequence ID" value="AAZ68106"/>
        <property type="gene ID" value="Ecaj_0055"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0055"/>
    <dbReference type="eggNOG" id="COG0162">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024003_0_3_5"/>
    <dbReference type="OMA" id="YMMAKDS"/>
    <dbReference type="OrthoDB" id="402899at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02006">
        <property type="entry name" value="Tyr_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024107">
        <property type="entry name" value="Tyr-tRNA-ligase_bac_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="PTHR11766"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000234705" description="Tyrosine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="348"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="235"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02006"/>
        </source>
    </evidence>
    <sequence length="418" mass="47358" checksum="2037889F88DBB49D" modified="2005-09-27" version="1">MKLESQFLSFLYSRGYFNQCTNVSALDQLMSKQCVPTYIGFDCTAKSLHVGSLVQIMILRYLQKFGHKPIVLLGNGTTKIGDPSGKDKSRTMLCSDEIEENALGIYDVLKKFIKFGSGSTDGLLVCNAEWLDDLNYIEFLRNIGRHFSVNNMLTFDSVRLRLEREQNLSFLEFNYMLLQSYDFVELNRRYNCLLQIGGSDQWGNIVSGVELGRKLQLSELFGLTTNLVLTSSGEKMGKTAQGAVWLNGDMYSPVDYWQYFRNVRDEDVGRFLKLFTELPLDKIKELELLQGHEINEAKKMLATEATKICHGEEIAKNIAADALKVFECNDDSGLSTFYVDRCDVDLGLPIIKLLQISGLEKSSSSARRLINDKGCKINDVVVLDVNYKLSLEHFCNASYVKLSCGKKRHLKIMLKSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT52</accession>
    <name>Q3YT52_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ankyrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304181.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0052"/>
    <dbReference type="EnsemblBacteria" id="AAZ68103">
        <property type="protein sequence ID" value="AAZ68103"/>
        <property type="gene ID" value="Ecaj_0052"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0052"/>
    <dbReference type="HOGENOM" id="CLU_425628_0_0_5"/>
    <dbReference type="OMA" id="YYLIMSL"/>
    <dbReference type="OrthoDB" id="541690at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="SSF48403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="515"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="548"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="613" mass="70527" checksum="DD5687FA953748C9" modified="2005-09-27" version="1">MLMMHKIAVTRYVKPLILSKILNEDKSFLKMLESLESEDILLLKNELGTVFYYLIMSLYDISGIKNNSSNTLRARLKELLSEAIDQSSMDQGRVVASQVSDIKDITSKLKLAYQSISNSVYRTLFNVLDSDETSEEEVDPQILRQRQLEYKAQMFECVIQAVRILADKINKAVAEQKISPEQVSEFLAYSDRPCDPLVPDAMSALAKLYFLIEDPQLSELAYSLVKEFLIGFSQYRLDKCGRNALHYAVNICNTKKQESFLCEIIQLLIYNRVEILNELDVDGNNPMHYVACAPYLNMDIVKYFLKNAPAMIVQQNSHGDTPLHIMSSVYLINFAKILSFYNITYRDNMWQLKDVMDLSLSEMRDVVMSVRGRDEELSSEIKNYLVQCVDAYRLLLTSVPLWRLFEVRDERGHTVYDIMTSAITSVGDGNLKALLEDFSEMSSRLPIYDSRIHAQNELCVKLCFDKKNIGKNRYGHSLYKYTKYIHDLIYSKFAKISESRARCHSLKKERNGFKFVSLFVIAVFILLCVLNAVLHFKERDILWCEKGVYRSVLFAVISIVFFTAVCVAYALYAKQIDCCNDKAMMYEKENIRGVLLSHLNIEAIGSTKEINIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT84</accession>
    <name>Q3YT84_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Conjugation TrbI-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0020</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrbI/VirB10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304149.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0020"/>
    <dbReference type="EnsemblBacteria" id="AAZ68071">
        <property type="protein sequence ID" value="AAZ68071"/>
        <property type="gene ID" value="Ecaj_0020"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0020"/>
    <dbReference type="eggNOG" id="COG2948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041915_0_0_5"/>
    <dbReference type="OMA" id="FVNQDIV"/>
    <dbReference type="OrthoDB" id="1093154at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16429">
        <property type="entry name" value="VirB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005498">
        <property type="entry name" value="T4SS_VirB10/TraB/TrbI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042217">
        <property type="entry name" value="T4SS_VirB10/TrbI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03743">
        <property type="entry name" value="TrbI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="354"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="445" mass="48408" checksum="8378D32F442C56AC" modified="2005-09-27" version="1">MSEENQNNQYTEIEESVNVVGVNKGKKFIIIGIILAGLGFAYYYFFIGKNSENDLNKPQSTEEVDIEKLLKESTQPAQEVSPAINIPPQLPELPPLVSPSLPSIPTVEKPKVLEIPKIPEVKQKQPPVQPQPKPETDVLPKIPLPAQNTIEIAAPIAPTTTGYDKERRATSMLAISGGQEVRAEGEESTISNVINNNNNSIISLQPTSSPNVVATKVNNLELTILQGKIIDVVLETAINSDLQGTLRGIVARDVYAEASNIVMIPKGSRLIGNYSFNAGPGKTRVQISWNRVILPHGIDISLDGNGTDELGRQGASGVVNTKIGNILTSTILLAGVSIATAYATSKIPEINDHPIIESKDKDTKDEDKDKDKDKNKDKDKTKTTLPVKILSQAVDDFSNSIKDIIKRYSDNNPTIYVDQGTLLKVFVNKDIVFPKAAVRGINIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT87</accession>
    <name>Q3YT87_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Gp140</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304146.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0017"/>
    <dbReference type="EnsemblBacteria" id="AAZ68068">
        <property type="protein sequence ID" value="AAZ68068"/>
        <property type="gene ID" value="Ecaj_0017"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0017"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_399950_0_0_5"/>
    <dbReference type="OMA" id="EIESHQG"/>
    <dbReference type="OrthoDB" id="2113859at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="558"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="688" mass="73675" checksum="92326603996B35DE" modified="2005-09-27" version="1">MDIDNNNVTTSSTQDKSGNLMEVIMRILNFGNNSDEKVSNEDTKVLVESLQPAVNDNVGNPSSEVGKEENAPEVKAEDLQPAVDGSVEHSSSEVGKKVSETSKEESTPEVKAEDLQPAVDGSIEHSSSEVGEKVSKTSKEESTPEVKAEDLQPAVDDSVEHSSSEVGEKVSETSKEENTPEVKAEDLQPAVDGSIEHSSSEVGEKVSKTSKEESTPEVKAEDLQPAVDDSVEHSSSEVGEKVSETSKEENTPEVKAEDLQPAVDGSVEHSSSEVGEKVSKTSKEESTPEVKAEDLQPAVDDSVEHSSSEVGEKVSETSKEENTPEVRAEDLQPAVDGSVEHSSSEVGEKVSETSKEESTPEVKAEDLQPAVDSSIEHSSSEVGKKVSETSKEESTPEVKAEDLQPAVDGSVEHSSSEVGEKVSETSKEENTPEVKAEDLQPAVDGSVEHSSSEVGEKVSETSKEENTPEVKAEDLQPAVDGSVEHSSSEVGEKVSETSKEESTPEVKAEDLQPAVDDSVEHSSSEVGEKVSETSKEESTPEVKAEDLQPAVDGSVEHSSSEVGEKVSETSKEESTPEVKAEVQPVADGNPVPLNPMPSIDNIDTNIIFHYHKDCKKGSAVGTDEMCCPVSELMAGEHVHMYGIYVYRVQSVKDLSGVFNIDHSTCDCNLDVYFVGYNSFTNKETVDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT92</accession>
    <name>Q3YT92_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304141.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0012"/>
    <dbReference type="MEROPS" id="S78.001"/>
    <dbReference type="EnsemblBacteria" id="AAZ68063">
        <property type="protein sequence ID" value="AAZ68063"/>
        <property type="gene ID" value="Ecaj_0012"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0012"/>
    <dbReference type="eggNOG" id="COG3740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073043_2_1_5"/>
    <dbReference type="OMA" id="RFANTEK"/>
    <dbReference type="OrthoDB" id="1734449at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006433">
        <property type="entry name" value="Prohead_protease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04586">
        <property type="entry name" value="Peptidase_S78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01543">
        <property type="entry name" value="proheadase_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1188">Viral release from host cell</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="177" mass="19687" checksum="DD6C3901FCC8E48D" modified="2005-09-27" version="1">MQQKTCNLFLSKKNIKQSGTFSGYASVFNVIDRQKHVILPGAFSNTIKDLNKVKLLWQHNSAEPIGNITNMTENNIGLHITANLLLDLQKGKEAYLMLKNGIINALSIGYSVIEDYTDVKTGIRVLKKISLWEISLVTFPANTHSKVTDVKNINADLATSFTRAKSALDELSSMINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT94</accession>
    <name>Q3YT94_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 9">Uroporphyrinogen decarboxylase</fullName>
            <shortName evidence="8">UPD</shortName>
            <shortName evidence="8">URO-D</shortName>
            <ecNumber evidence="4 8 9">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hemE</name>
        <name evidence="12" type="ordered locus">Ecaj_0010</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>4 H(+) + uroporphyrinogen III = 4 CO2 + coproporphyrinogen III</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8 9">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 10">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="4 8 9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304139.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0010"/>
    <dbReference type="EnsemblBacteria" id="AAZ68061">
        <property type="protein sequence ID" value="AAZ68061"/>
        <property type="gene ID" value="Ecaj_0010"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0010"/>
    <dbReference type="eggNOG" id="COG0407">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040933_0_0_5"/>
    <dbReference type="OMA" id="LWLMRQA"/>
    <dbReference type="OrthoDB" id="1104410at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00218">
        <property type="entry name" value="URO_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 8 9" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen_deCOase" evidence="11">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="20"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="334" mass="37602" checksum="3EE18965CBBDD628" modified="2005-09-27" version="1">MLKTIVSKSKQQEVPVWFMRQAGRYLPEYHEVARKAGSFLELCYTPELVKEVTLQPVKRFGLDAAIIFSDILVIPDALGCNVEFTKENGPSLQLISSHKEIDVPEKAVIDHLKKVFEGIKEVRKSLQKSKPLIGFAGAPWTIASYMIGRDKNFSKIREMSYLQDKELGKIIKKITKVTISYLIKQIESGVDIIQIFDSNAGIVSANEFRKWIINPTREIVSSIRKLYPEFPIIGFPKGAGIMYKQFSEETEVSVTSIDYNVPISWAKDNISSILQGNIDPYLVAYDKSKAISQTKDLINIMKDEPFIFNLGHGIIPSTPIANIEALIETVKSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQN6</accession>
    <name>Q3YQN6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305042.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0938"/>
    <dbReference type="EnsemblBacteria" id="AAZ68969">
        <property type="protein sequence ID" value="AAZ68969"/>
        <property type="gene ID" value="Ecaj_0938"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0938"/>
    <dbReference type="HOGENOM" id="CLU_818198_0_0_5"/>
    <dbReference type="OMA" id="CETHANK"/>
    <dbReference type="OrthoDB" id="1246196at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="325" mass="35869" checksum="22BDCB6B96F79AE3" modified="2005-09-27" version="1">MLDNSNKKGKGIAEEQLLKTLNYSLYKLNSQDVTRKKIVLAVSLLYALVFASGAILQSGILGISKENTIFLGCLVEFVALLLCISFLVTGLYNVLYIEKERKKLNEKIKKCKGQYDTVSFSNDVAEFCDGHANKFDLTGGFLTTIMQAVAVFSLMVTTMFDVTKIPYGTAFNLQGIVDTVGNIFFLIAASMFLISYLIRCKNSKDRDGRSSGSPAQAFIFSSIFFGTFLIFAGKVLLSFECRAGAMYTNSLGPFGMDALPLGFITRCIGMMIFCTGYALMLHFSIKSNKELSAKVQIDDEQYIASGMEESYVQHFSDKDQILSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQQ1</accession>
    <name>Q3YQQ1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0923</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305027.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0923"/>
    <dbReference type="EnsemblBacteria" id="AAZ68954">
        <property type="protein sequence ID" value="AAZ68954"/>
        <property type="gene ID" value="Ecaj_0923"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0923"/>
    <dbReference type="HOGENOM" id="CLU_125881_0_0_5"/>
    <dbReference type="OMA" id="FLIQQNG"/>
    <dbReference type="OrthoDB" id="1816603at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="184" mass="20647" checksum="D4A31DDCB1CDE2A3" modified="2005-09-27" version="1">MGLYYNEYQNDHQIDFLIQQNGHSNVDIYHGTLHINGNEGKYYASASIVNDLTGDKGTINILNHGVKGIYSNESPKCDLEITLEHYDPASSDNIITSINKSFIVSVFSNLQDCLLIDSHNPLLMDPSKVEQINKEPVIAVLVHDGIDTKKIRDSFFNEKPENKEHKAGGCTYVYSHGKTETKCE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQQ3</accession>
    <name>SECA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translocase subunit SecA</fullName>
            <ecNumber evidence="1">7.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secA</name>
        <name type="ordered locus">Ecaj_0921</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + cellular proteinSide 1 = ADP + phosphate + cellular proteinSide 2.</text>
            <dbReference type="EC" id="7.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">May bind 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Distribution is 50-50.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecA family.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305025.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YQQ3"/>
    <dbReference type="SMR" id="Q3YQQ3"/>
    <dbReference type="STRING" id="269484.Ecaj_0921"/>
    <dbReference type="PRIDE" id="Q3YQQ3"/>
    <dbReference type="EnsemblBacteria" id="AAZ68952">
        <property type="protein sequence ID" value="AAZ68952"/>
        <property type="gene ID" value="Ecaj_0921"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0921"/>
    <dbReference type="eggNOG" id="COG0653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005314_3_0_5"/>
    <dbReference type="OMA" id="MVHYDVQ"/>
    <dbReference type="OrthoDB" id="212453at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008564">
        <property type="term" value="F:protein-exporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18803">
        <property type="entry name" value="SF2_C_secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01382">
        <property type="entry name" value="SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020937">
        <property type="entry name" value="SecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011130">
        <property type="entry name" value="SecA_preprotein_X-link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011116">
        <property type="entry name" value="SecA_Wing/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036266">
        <property type="entry name" value="SecA_Wing/Scaffold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036670">
        <property type="entry name" value="SecA_X-link_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="PTHR30612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01043">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07516">
        <property type="entry name" value="SecA_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00906">
        <property type="entry name" value="SECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00958">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81767">
        <property type="entry name" value="SSF81767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81886">
        <property type="entry name" value="SSF81886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00963">
        <property type="entry name" value="secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01312">
        <property type="entry name" value="SECA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000320795" description="Protein translocase subunit SecA">
        <location>
            <begin position="1"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="104"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="848"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="850"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="859"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="860"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01382"/>
        </source>
    </evidence>
    <sequence length="862" mass="98923" checksum="A7F7A5B2F8B09CEE" modified="2005-09-27" version="1">MLSIAHKIFGSANSRIIKSFYKVVQHINAIEHEFQLLSNEALKNKTIEFKEELKNGKTLDDILVPAFAVVREASKRVLNMRHFDVQLIGGMVLHKGMISEMKTGEGKTLVATLAAYLNALEGKGVHIVTVNDYLAKRDAEWMGELYDALGITVGCILTDTNDLERKNAYQCDILYSTNNNLGFDYLRDNMKFSRSEMVQRGFNYAIVDEVDSILIDEARTPLIISGQVDQDIKMYKKIDNLIYELVEEDYELEEKSKNIFLTEAGTTKIENLLTKHNLIPSNTSLYDIDNIIIMHYIIQALRAHKIFALDKDYIIKNGNIVIIDEFTGRMMDGRRYSDGLHQALEAKEKLNINSENQTLASTTFQNYFRMYTKLSGMTGTAATESEEFLGIYNLQVVQIPTNIPVQRIDLNDDIYCTEEEKFSAVIKFISECHQKLQPVLVGTVSIEKSEMLSKLLTQNKLKHSVLNARYHEQEAYIIAQAGIPGTITIATNMAGRGTDIQLGGNLKMLAKTALANTTDKEAIEIKYKQLSEKVKKDKEIAIQAGGLCVIGTERHESRRIDNQLRGRSGRQGDPGLSKFFLSLEDDLLRIFGSDKIKGVLKKLGMKKDEAIQHTWISRSIEKAQHKVESRNYDIRKSLLKFDNVINEQRKVVFDQRNRILDNDSYDISIIYRDLNSEIVNSIIHDKYYNLDDETYKILSSEFTRIYALTLDYSIISELESKEKVIEYLNKIVDEHFTQKIEEFKSRDQKLWDYAVKKVMIMSLDYLWRDHLAALDSLKCGINLRSIAQKDPLNEFKAEAFSMLENMMNKFYELITQRLSHLRFDIELSETQIPEYNINHTKISRNEKCPCGSGKKFKHCHGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQS5</accession>
    <name>Q3YQS5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305006.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0899"/>
    <dbReference type="EnsemblBacteria" id="AAZ68930">
        <property type="protein sequence ID" value="AAZ68930"/>
        <property type="gene ID" value="Ecaj_0899"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0899"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="QYHKVIG"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="283" mass="32496" checksum="18D957EEED82515C" modified="2005-09-27" version="1">MYKLYYLSFIISLAQLLFSGFAFSIDKNNNIHGSYITIKYQPTISNFKNFHIKETDFDTEDPIGFDIIAPNTNFDFLKHNYNFSVLYHKDSYKFYENDLSGLALSIGLLVKNLRIEFEGSYKNFDTKRLAYYHSREGHKFFAIPRTSNFGVIPNEDNYTVAKNNGISIISNIINLCSETKFKNFTPYICLGIGGDFIEIFDVMRVKFTYQGKVGISYPITPKLVLSISGQYHKVIGNKFKFLPLIQPVALKRTDNSPEDKDVTALLTLDLEHFSSEIGLSFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQS7</accession>
    <name>Q3YQS7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305004.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0897"/>
    <dbReference type="EnsemblBacteria" id="AAZ68928">
        <property type="protein sequence ID" value="AAZ68928"/>
        <property type="gene ID" value="Ecaj_0897"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0897"/>
    <dbReference type="HOGENOM" id="CLU_1755959_0_0_5"/>
    <dbReference type="OrthoDB" id="2150934at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="148" mass="17570" checksum="AF3545DF658F05BB" modified="2005-09-27" version="1">MNNKVLQNEVLLLLALPYSLHNSIDIEYNEQPIKKLSRLNKNTIKSQLKHLNYKYILLKLKKISSIGWFLKFSNDYSNSQVIYYLRTSLSKLVSTIALFDFKNIFLNYKVLTDSLRNFSANYYFSKHHNITNCFCVSRAYFYFLSCSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQU3</accession>
    <name>Q3YQU3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0881</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68912">
        <property type="protein sequence ID" value="AAZ68912"/>
        <property type="gene ID" value="Ecaj_0881"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0881"/>
    <dbReference type="HOGENOM" id="CLU_079586_0_0_5"/>
    <dbReference type="OMA" id="KHIAAKT"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="305" mass="34756" checksum="15B50A8C70441649" modified="2005-09-27" version="1">MVIQNILIGDNSNITIFFTEKNEIENFTKIFTVLDKNKAAKTLFNNEVNIQYQDMSATLTSSTHFEFSDLNKIITHMLQHNFTISTNIIAQSLEQGCNILKTSNLVICRFNNQPLYCINVSIRNNTIILHPISTQYSDLSSEDNKKLINALQTHTNTNDITTDNKQHAILISINTVIYEVLQSLVNILIKVQIIEENDKEKTLQQLTKLAFHDFTSNELQIVKNIAQYPTDHPLSKYKNIAKNVENIFSHLASTQSLDSSSAKLLQDAINNTGEFSTAPHIIMRSFNKLNKNFHDQIQNIINQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQU8</accession>
    <name>Q3YQU8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Cell division protein FtsA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsA</name>
        <name evidence="8" type="ordered locus">Ecaj_0876</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Cell division protein that is involved in the assembly of the Z ring. May serve as a membrane anchor for the Z ring.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Self-interacts. Interacts with FtsZ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="5">Localizes to the Z ring in an FtsZ-dependent manner. Targeted to the membrane through a conserved C-terminal amphipathic helix.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the FtsA/MreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304983.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0876"/>
    <dbReference type="EnsemblBacteria" id="AAZ68907">
        <property type="protein sequence ID" value="AAZ68907"/>
        <property type="gene ID" value="Ecaj_0876"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0876"/>
    <dbReference type="eggNOG" id="COG0849">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037850_3_0_5"/>
    <dbReference type="OMA" id="DGIIRHT"/>
    <dbReference type="OrthoDB" id="1044637at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02033">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020823">
        <property type="entry name" value="Cell_div_FtsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003494">
        <property type="entry name" value="SHS2_FtsA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02491">
        <property type="entry name" value="SHS2_FTSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003101">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00842">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01174">
        <property type="entry name" value="ftsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsA" evidence="7">
        <location>
            <begin position="15"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR003101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="411" mass="44644" checksum="639AB6F6D1BFF378" modified="2005-09-27" version="1">MLHSAVITGSGRGIFAIVDLGSTKIVCFIIRVSRNSSPEVIGIGYKAAEGISGGTITNMESASYSILSCIESAKKMAQESVISQVFVNISGCDICSTNVVNEINAVIHEISDIDIRNIMLQTYDKCRDDQIIIHNIPIVYSLDDLNNITELKGLYGSKLKASVHVIAASKFALLNIENCITSCSSLGVTGCIAEPYSSGLSCLTDDEKEIGAMILDIGGRYTSLGVFYKGKLIYADSIPLGGMHITNDIAYGLCISVRDAERIKVLYGDAMLISPDKDGIIEADVGEDEIISVVRSDLVKIIKPRVEEIFDIVNSRIVKQKDLINRVVITGGSSQLINIKEVASYVLKKQVRIGLPLELKGISDDYKRNPSFAAAIGTVLLVSNRIHNKRKPIPRKNNILKKLLNLIPVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQW4</accession>
    <name>Q3YQW4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytochrome c biogenesis protein, transmembrane region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304968.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0860"/>
    <dbReference type="EnsemblBacteria" id="AAZ68891">
        <property type="protein sequence ID" value="AAZ68891"/>
        <property type="gene ID" value="Ecaj_0860"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0860"/>
    <dbReference type="eggNOG" id="COG4232">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014657_0_1_5"/>
    <dbReference type="OMA" id="CKEMERF"/>
    <dbReference type="OrthoDB" id="1055204at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02953">
        <property type="entry name" value="DsbDgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035671">
        <property type="entry name" value="DsbD_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="409" mass="46042" checksum="206914E368F05CAA" modified="2005-09-27" version="1">MFFSVNLDLIYILISALLGGVILNCMPCVFPVLSLKIMSLIKSAKYKKKISVKGEGLSYTLGVMTSMFVLSSVLLILRHFGHLVSWGYQMQSPMLITLLMYVMFLMGLSFSGFYDFPFIFPNLNGVNTKKEGLIGSFFVGVLSTFIATPCTVPFMVSAITVALSQPSVYSLLIFQVLGFGMALPYLLLSFFPGLLKILPKPGKWMEVLQRFLAFPLYFSAAWLLCILVKQKGTEILFAVLSCAILFVMGIWIMKLVKSWKPVSKFVIFLCLLLLAISPLYFKSVKEFVMQNREPKRIATMEFSQAKLDQLLKEKKTVLLSVGADWCLTCKVNEQVFQLSTVQALFAKKNVYYMKGDLTTKNPEVTAYINQLDKNSIPLYVLYVGGVKVKVLPQVLSEKTVIDIIKQYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQW7</accession>
    <name>Q3YQW7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex</fullName>
            <ecNumber evidence="5 9">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0857</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">E2 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the second step in the conversion of 2-oxoglutarate to succinyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + succinyl-CoA = (R)-N(6)-(S(8)-succinyldihydrolipoyl)-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + CoA</text>
            <dbReference type="Rhea" id="RHEA:15213"/>
            <dbReference type="Rhea" id="RHEA-COMP:10581"/>
            <dbReference type="Rhea" id="RHEA-COMP:10582"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="2.3.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="9">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="5 9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304965.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0857"/>
    <dbReference type="EnsemblBacteria" id="AAZ68888">
        <property type="protein sequence ID" value="AAZ68888"/>
        <property type="gene ID" value="Ecaj_0857"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0857"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_0_0_5"/>
    <dbReference type="OMA" id="DMAGATF"/>
    <dbReference type="OrthoDB" id="1626282at2"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="11">
        <location>
            <begin position="111"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="400" mass="43850" checksum="A2A7AEE8E015194D" modified="2005-09-27" version="1">MSEIQVKAENLGGESILEAPIRVSVNVGDTVKQGDMLFIIETDKTSLEIVSPEDGVIGEIFVTDEAMIQRGQVLCTINTVQSSAVESSDTSSAHNATTTAADCMQQFIQKKDAPSATKLMKENSIDRDQISGSGVSGRITKSDVLNYMKSTASEGSNINRLAVVSEGKREDRVKMSKIRQVIAARLKESQNTAAILTTFNEVDMKNVMDLRAQYRENFEKKYSIKLGFMSFFIKAVILALKELPVINAEISGNEIVYKHYYDIGIAVGTDKGLVVPVIRDADKMSFSELELTLAALGKKAREGKLEVSDMAGATFTITNGGVYGSLLSTPIINPPQSGILGMHSIQKRPVAVDDKTIEIRPMMYIALSYDHRIVDGQGAVTFLVRVKQYIEDPSRMFLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQX9</accession>
    <name>Q3YQX9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Periplasmic serine endoprotease DegP-like</fullName>
            <ecNumber evidence="5">3.4.21.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0845</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val.</text>
            <dbReference type="EC" id="3.4.21.107"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.107" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304953.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0845"/>
    <dbReference type="EnsemblBacteria" id="AAZ68876">
        <property type="protein sequence ID" value="AAZ68876"/>
        <property type="gene ID" value="Ecaj_0845"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0845"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_1_0_5"/>
    <dbReference type="OMA" id="NRSVSMR"/>
    <dbReference type="OrthoDB" id="741829at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011782">
        <property type="entry name" value="Pept_S1C_Do"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02037">
        <property type="entry name" value="degP_htrA_DO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5010603602" description="Periplasmic serine endoprotease DegP-like" evidence="5">
        <location>
            <begin position="23"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="255"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="365"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR611782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="471" mass="50908" checksum="34FF9489E9CDC091" modified="2005-09-27" version="1">MRRFLLLLTLILANIPISNLIAQGTEQYDPRSGFSKLIKESTPAVVNVSIVHDVTNEQFPLITIEELLRSILEGKQIKKDVPQEILSAGSGFVVDESGIIVTNYHVVHNAKEVYITFSNNKSIPAKILGVDPQTDLAVLKVEVNEKLPYLDFGDSDTAMVGDWVVAIGNPFGLGGSASIGIISARARDLNIGTATEFLQTDAAINKGNSGGPLFNVDGKVIGINTAILSTQKGGGNIGVGFAIPSNSAVPIIKVLSQGKKVEHGWLGVVMQPITEELVEPFKLKEVSGALITNIVKGSPADKAKLLPGDIILEFNGTKINSISQLHQLVLRSEANNEVTLVVSRNGSIINISVKIGKFENPDPSENELPKDSVQSHELGLTVGNIKHNQIMSNDTTEEEVKGVMILNVDYTSNASTKNIRKGDIILQINQSPINNLEDFKNVMKKVRKNKSAALLISRDNISMFVTVKLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQY3</accession>
    <name>Q3YQY3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304949.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68872">
        <property type="protein sequence ID" value="AAZ68872"/>
        <property type="gene ID" value="Ecaj_0841"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0841"/>
    <dbReference type="HOGENOM" id="CLU_148745_1_0_5"/>
    <dbReference type="OrthoDB" id="1910088at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007039">
        <property type="entry name" value="TrbC/VirB2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04956">
        <property type="entry name" value="TrbC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="121" mass="12777" checksum="E5E6C1629B986B43" modified="2005-09-27" version="1">MLRAVFVFLVIMGFISVAPLTFASGTEKSSNADAQDEVSKVICNVIVFVQKLGLPIMTGVILGSSIMAIFGRLPWPAIVMLVVFTAIFFGAGKLVSKFVAGVNNTLTAGSDCTSNTSIEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQY8</accession>
    <name>Q3YQY8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0836</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68867">
        <property type="protein sequence ID" value="AAZ68867"/>
        <property type="gene ID" value="Ecaj_0836"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0836"/>
    <dbReference type="HOGENOM" id="CLU_2492929_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="86" mass="10089" checksum="AE09B36DF0D87CAA" modified="2005-09-27" version="1">MCSSKRTCFFINICRKSTVVLVFYCAKCKEVLFMILKCIDCINVNAYFLITSYIINYTLSFFGDFIGSAEKFCKKLFIGFLKFEMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQZ5</accession>
    <name>Q3YQZ5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Protein ApaG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">apaG</name>
        <name evidence="4" type="ordered locus">Ecaj_0829</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304937.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0829"/>
    <dbReference type="EnsemblBacteria" id="AAZ68860">
        <property type="protein sequence ID" value="AAZ68860"/>
        <property type="gene ID" value="Ecaj_0829"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0829"/>
    <dbReference type="eggNOG" id="COG2967">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128074_1_0_5"/>
    <dbReference type="OMA" id="YVSGCNL"/>
    <dbReference type="OrthoDB" id="1835593at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00791">
        <property type="entry name" value="ApaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007474">
        <property type="entry name" value="ApaG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036767">
        <property type="entry name" value="ApaG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023065">
        <property type="entry name" value="Uncharacterised_ApaG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04379">
        <property type="entry name" value="DUF525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110069">
        <property type="entry name" value="SSF110069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51087">
        <property type="entry name" value="APAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApaG" evidence="3">
        <location>
            <begin position="6"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00017693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="134" mass="15366" checksum="96E0C90B38B4CBC5" modified="2005-09-27" version="1">MTLQYYSMTKLIEVKVVPSYLEEQSAPHENCYIWLYNIRVKNRSNSTVQLLRRSWKIIDSTGIINEVTGLGVIGKQPILKPGEFFEYTSGAYLSTPSGMMHGEYQFMDEDAAQVFYVNIPVFSLDSPYVNTKPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR11</accession>
    <name>Q3YR11_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="2 7">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">purD</name>
        <name evidence="10" type="ordered locus">Ecaj_0813</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-phospho-beta-D-ribosylamine + ATP + glycine = ADP + H(+) + N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="2 7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304921.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0813"/>
    <dbReference type="EnsemblBacteria" id="AAZ68844">
        <property type="protein sequence ID" value="AAZ68844"/>
        <property type="gene ID" value="Ecaj_0813"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0813"/>
    <dbReference type="eggNOG" id="COG0151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027420_3_0_5"/>
    <dbReference type="OMA" id="KATVCKY"/>
    <dbReference type="OrthoDB" id="932854at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PTHR43472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="9">
        <location>
            <begin position="107"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="421" mass="46092" checksum="69E0EF8816FAAA2A" modified="2005-09-27" version="1">MNVLVIGSGGREHSMLHHIRKSPLLNKLFIAPGRQGMAGLADIIDIDINNTIDVIQVCKKEKIELVVIGPEAPLMNGLSDTLTEEGILVFGPSKAAARLESSKGFTKELCMRYGIPTAKYGYFVDAGPAYKFIDKHKLPLVVKADGLAQGKGTIICHTHEEAYNAVDAMLVHHKFGEAGYAIIIEEFLEGKEISFFTLVDGSNPVILGVAQDYKTIGDNNKGPNTGGMGSYSKPNIITQEMEHIIIQKIIYPTIKAMFNMNIQFRGLLFAGIIIKKNEPKLLEYNVRFGDPETQSILPRLNSDFLKLLSLTAKGKLGNESVELSKKAALCVVVASRGYPGEYKKNSIINGIENIEKLPNIQLLHAGTRREGNNWVSDAGRVINVVAQGENLASAKHQAYAALDLLDWPDGIYRYDIGSCAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR26</accession>
    <name>Q3YR26_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative ThiS sulfur transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0798</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0798"/>
    <dbReference type="EnsemblBacteria" id="AAZ68829">
        <property type="protein sequence ID" value="AAZ68829"/>
        <property type="gene ID" value="Ecaj_0798"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0798"/>
    <dbReference type="eggNOG" id="COG2104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174611_2_0_5"/>
    <dbReference type="OMA" id="DIVDIVY"/>
    <dbReference type="OrthoDB" id="2084255at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010035">
        <property type="entry name" value="Thi_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01683">
        <property type="entry name" value="thiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="58" mass="6476" checksum="F8BBB65985AC6E9C" modified="2005-09-27" version="1">MVFESGLTLSQILNMCSYDTNLPFAVAVNRNLIIRDKYSSTYLNNGDIVDIVYPMQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR27</accession>
    <name>Q3YR27_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thiazole synthase</fullName>
            <ecNumber evidence="4">2.8.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0797</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH = 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + 2 H(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:26297"/>
            <dbReference type="Rhea" id="RHEA-COMP:12908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12909"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="ChEBI" id="CHEBI:77846"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:90778"/>
            <dbReference type="EC" id="2.8.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.10" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304905.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0797"/>
    <dbReference type="EnsemblBacteria" id="AAZ68828">
        <property type="protein sequence ID" value="AAZ68828"/>
        <property type="gene ID" value="Ecaj_0797"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0797"/>
    <dbReference type="eggNOG" id="COG2022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062233_1_0_5"/>
    <dbReference type="OMA" id="PHNFQLI"/>
    <dbReference type="OrthoDB" id="784095at2"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990107">
        <property type="term" value="F:thiazole synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04728">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033983">
        <property type="entry name" value="Thiazole_synthase_ThiG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008867">
        <property type="entry name" value="ThiG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34266">
        <property type="entry name" value="PTHR34266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05690">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0784">Thiamine biosynthesis</keyword>
    <feature type="domain" description="ThiG" evidence="6">
        <location>
            <begin position="4"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="261" mass="28564" checksum="E8BD37FE2EB191AF" modified="2005-09-27" version="1">MLNLYGVLLQSRLLLGSAMYPSPAILCESIKLSGAEVVTVSLSRQMPSENGGNDFWNIVKESGCHILPNTAGCSTVDDAVLMSQMAREIFGTNWIKLELIGDEYTLYPDLILLCEAAKELINQGFEVFPYCTDDLIVCKKLVDYGCRVLMPGLAPIGSGCGILNMYNLKLLRHRFPETTMIADAGIGRPSDAVMIMEMGFDAVLINTAIARSIVPQDMSCAFKYAVEAGRIAYRAGIIHKKDFAVSSTPLIDTPFWCNTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR35</accession>
    <name>Q3YR35_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="10">tRNA 2-methylthioadenosine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0789</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304897.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0789"/>
    <dbReference type="EnsemblBacteria" id="AAZ68820">
        <property type="protein sequence ID" value="AAZ68820"/>
        <property type="gene ID" value="Ecaj_0789"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0789"/>
    <dbReference type="eggNOG" id="COG0621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_1_1_5"/>
    <dbReference type="OMA" id="HFHIPLQ"/>
    <dbReference type="OrthoDB" id="397139at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035596">
        <property type="term" value="F:methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006467">
        <property type="entry name" value="MiaB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01579">
        <property type="entry name" value="MiaB-like-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="123"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="411" mass="46832" checksum="0CE9E65BFDA55D2C" modified="2005-09-27" version="1">MSDVITFGCRLNFYESEVIKNNLRKAQLEDVIVIHSCAVTNEAERQVRSKIRKLYNNNANVKIIVAGCAAQLNPELYINMPGVIKVLGNQDKLKYESYVTEGKVIVSKIEDSREIVQNSVNRFSGKSRALIEIQNGCNHECTFCVITKARGDNRSLNIEDIITKVRDCVNNGYNEVVFTGVDISDFGLDIYGQRVLGTMVKRVLSAVPQLRRLRLSSIDVAEIEDDLVDIIVNEPRFMPHLHLSLQSGNNLILKRMKRRHNREQVIDFCNKIRNKRKEVAFGADIIVGFPTETEEMFNDTVRLIEEANISYLHVFPYSRREGTPAARMPQVIQEVKKRRVKYLLEFAEKRLHSFYNTLLHTKQSVIVEKSGTGRAENFALVKFANNDVKLQSIVEVKITTVEGNCLIAQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR46</accession>
    <name>Q3YR46_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane translocase, subunit Tim44</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304886.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0778"/>
    <dbReference type="EnsemblBacteria" id="AAZ68809">
        <property type="protein sequence ID" value="AAZ68809"/>
        <property type="gene ID" value="Ecaj_0778"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0778"/>
    <dbReference type="eggNOG" id="COG4395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086329_1_0_5"/>
    <dbReference type="OMA" id="WKLVGTG"/>
    <dbReference type="OrthoDB" id="1536134at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016985">
        <property type="entry name" value="UCP031890_Tim44-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031890">
        <property type="entry name" value="UCP031890_transporter_Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tim44" evidence="1">
        <location>
            <begin position="65"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="212" mass="24409" checksum="E790335C69D2B302" modified="2005-09-27" version="1">MVELAIYALLAAFIFFRLYNSLGKTSGLQPNNHSQAISIVVEDTQEYDDAEIEIESVMDTCNKDEKSIINQIKKKDKEFSLKHFMDGSIKAFEIIIKAFNEGNVHILSMLLDTNLYNSFSEEIENRKKLNHVYEDIIVSVTSHKIIKLDLSGNIAYITVKFITEQINFVKDKDDNVIQGSTSKINKIEDVWTFRKDITLSNKKWYLNAIQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR54</accession>
    <name>Q3YR54_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304878.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0770"/>
    <dbReference type="EnsemblBacteria" id="AAZ68801">
        <property type="protein sequence ID" value="AAZ68801"/>
        <property type="gene ID" value="Ecaj_0770"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0770"/>
    <dbReference type="HOGENOM" id="CLU_2218941_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="106" mass="11792" checksum="12ADA2642E0210D4" modified="2005-09-27" version="1">MSSDFYLKVLLPIIVAVVLLVAALLIVCMISRCIQKSSSDRKVRDKLEMEEHGLLADESENDRNEAVQLVARPVPQLNVEHVENIFRCVVNSARESHNCMVVTSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR60</accession>
    <name>Q3YR60_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0764</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304872.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68795">
        <property type="protein sequence ID" value="AAZ68795"/>
        <property type="gene ID" value="Ecaj_0764"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0764"/>
    <dbReference type="HOGENOM" id="CLU_1419487_0_0_5"/>
    <dbReference type="OrthoDB" id="2225610at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="191" mass="21011" checksum="A07CCA17BA024563" modified="2005-09-27" version="1">MDNNTTTNTSTSANATESTTNTSTTSLQNLTQSTLNSTANNITQCTTSSTSNSTTPLTSNSTTPTSTTSNTSSHISIFIFFAFAILAFAFILRLLRNRFRCNDNSTTNHDTARTTHQNKENVKLTSNTRSQNEQNQDPDPDPAPQTPQHYDYSSFRSEFSDNDPPITLPSTDLALQPPDSIQPYCTEEHTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR64</accession>
    <name>PYRH_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uridylate kinase</fullName>
            <shortName evidence="1">UK</shortName>
            <ecNumber evidence="1">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Uridine monophosphate kinase</fullName>
            <shortName evidence="1">UMP kinase</shortName>
            <shortName evidence="1">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrH</name>
        <name type="ordered locus">Ecaj_0759</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP. Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UMP kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304868.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YR64"/>
    <dbReference type="SMR" id="Q3YR64"/>
    <dbReference type="STRING" id="269484.Ecaj_0759"/>
    <dbReference type="EnsemblBacteria" id="AAZ68791">
        <property type="protein sequence ID" value="AAZ68791"/>
        <property type="gene ID" value="Ecaj_0759"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0759"/>
    <dbReference type="eggNOG" id="COG0528">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033861_0_0_5"/>
    <dbReference type="OMA" id="PIIVFDM"/>
    <dbReference type="OrthoDB" id="1043239at2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04254">
        <property type="entry name" value="AAK_UMPK-PyrH-Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_B">
        <property type="entry name" value="PyrH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015963">
        <property type="entry name" value="Uridylate_kinase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02075">
        <property type="entry name" value="pyrH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000323841" description="Uridylate kinase">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in allosteric activation by GTP" evidence="1">
        <location>
            <begin position="25"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="141"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <sequence length="244" mass="26515" checksum="FE88DFA0A5C8A907" modified="2005-09-27" version="1">MSESSTPNVKYSRVLLKVSGEALMGEKGFGCDVEVLDKLSHDLKEVYDFGIQLCLVVGGGNIFRGASSSSPFGFERASNDYIGMLATMMNALSLQNALEKINVQSRVLSAIPITAVCETYIRRRAIRHLEKGRVVICAAGVGNPFFTTDTAAALRGIEMGCDAIFKGTQVDGVYSADPKKTVDAVRYDRISYRDLLSLDLKIMDVAAVSLAREHSVPIIVFNLGKRGSFADIIRGRGLYTTICN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR82</accession>
    <name>Q3YR82_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304850.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0741"/>
    <dbReference type="EnsemblBacteria" id="AAZ68773">
        <property type="protein sequence ID" value="AAZ68773"/>
        <property type="gene ID" value="Ecaj_0741"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0741"/>
    <dbReference type="HOGENOM" id="CLU_2117170_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="114" mass="12677" checksum="68B133FDD1FE9388" modified="2005-09-27" version="1">MSSAVEKIDSILDKPNIERQDILWLRNSLLAMQNRMADAVSRSKEQSFKDALNSLLNLHEIPIVGGKQREGTKESCEVDNVNVQNQDRDEKPSGEVDGVNVQGKNNCNNVKKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR94</accession>
    <name>Q3YR94_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304838.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68761">
        <property type="protein sequence ID" value="AAZ68761"/>
        <property type="gene ID" value="Ecaj_0729"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0729"/>
    <dbReference type="HOGENOM" id="CLU_2272948_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="102" mass="11832" checksum="6453293D1E78B782" modified="2005-09-27" version="1">MHQPVEVKVYSKAEQDKRLIENQAIIKNLKLEQDLLLKKLAKYKEDKACLLSEIQLLQQALETERLNGMQSARNLIESKMVRSDSAIYEIMFDMCSTLDKCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR97</accession>
    <name>Q3YR97_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0726</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304835.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68758">
        <property type="protein sequence ID" value="AAZ68758"/>
        <property type="gene ID" value="Ecaj_0726"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0726"/>
    <dbReference type="HOGENOM" id="CLU_1287147_0_0_5"/>
    <dbReference type="OrthoDB" id="2129017at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="214" mass="24695" checksum="C8C596A2AD330914" modified="2005-09-27" version="1">MDTQLIIIILLAIFSFLFALFIAVSIYKCCVSCNDIKEDYKEECVEGDIQKELELLKQRNKELEDTVRKLNTELIISRADFQKQCSEMTKSLKDMSNIDNLTIEVAVLHKNLIDFAKRMESDLTKSSSEKLQCLSSEILLKLYQFCHSVLIRGQRNVKNEAMAQDLEHVQKIMFSIITQLTERQQLQTEEVSPEHQVHSTSVSSYGARNINQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRA4</accession>
    <name>Q3YRA4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0719</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68751">
        <property type="protein sequence ID" value="AAZ68751"/>
        <property type="gene ID" value="Ecaj_0719"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0719"/>
    <dbReference type="HOGENOM" id="CLU_1319262_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="208" mass="24073" checksum="2ED2D5A1AFF3A386" modified="2005-09-27" version="1">MFYCLYEYLQKKSEKKENLLQQLSYNNKLHLKDLEKLIKLLDKRKTLYANYFSYVSIFIQAACLLCDDLLVACMDGPPMLEMLITKTSDDFVAAHHDIKLLAVKALKEKGMDDLMLGVCLLKHYGVLEMRFGQLMSCVCTVRSRYTCQKPFSQVCKQAECMLTSFYNMLEALKSEVNDRAKKRSIPSADIKIDGIILEKYGKYQQLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRB7</accession>
    <name>Q3YRB7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0706</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304815.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0706"/>
    <dbReference type="EnsemblBacteria" id="AAZ68738">
        <property type="protein sequence ID" value="AAZ68738"/>
        <property type="gene ID" value="Ecaj_0706"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0706"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_11_3_5"/>
    <dbReference type="OMA" id="MGDYFDL"/>
    <dbReference type="OrthoDB" id="1558163at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="273" mass="31214" checksum="806664E86FEEE8FA" modified="2005-09-27" version="1">MHLFCFGYGYTAKFLAQKLLPLKWKISGTSFNKISQDKNVNLLNYNFPLPESIFSDVTHVLISIPPEGDNVLQKYYKYLKEIKWLGYLSATNVYGDHNGNWVDETSATNPSTVSGINRLKQEKIWYNSNLPSHIFRLSGIYGPNRNVLCNLIQNNVKYIISDTIFSRIHVEDIANILFTSINNPNPGSIYNCADDLPTSYSNIIKYGSQLLNIKTPEPVLLEDLSDNMKKFYSERKLVSNFKIKNALNISLLYPSYIQGLQNLIKIEKPLSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRB8</accession>
    <name>Q3YRB8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cold shock-like protein CspA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0705</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304814.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0705"/>
    <dbReference type="EnsemblBacteria" id="AAZ68737">
        <property type="protein sequence ID" value="AAZ68737"/>
        <property type="gene ID" value="Ecaj_0705"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0705"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_4_1_5"/>
    <dbReference type="OMA" id="FICKDQG"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="10"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="87" mass="9948" checksum="C039E8C4C853E381" modified="2005-09-27" version="1">MSQRLVDDSTYTGYVKWFSSEKGYGFICKDQGDKVKNIGQDIKDIFVHITALQRSKIDGLREGQKVKYQLDKNNGKFSAVNLEVLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRB9</accession>
    <name>Q3YRB9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative sensor histidine kinase NtrY-like</fullName>
            <ecNumber evidence="4">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0704</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304813.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0704"/>
    <dbReference type="EnsemblBacteria" id="AAZ68736">
        <property type="protein sequence ID" value="AAZ68736"/>
        <property type="gene ID" value="Ecaj_0704"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0704"/>
    <dbReference type="eggNOG" id="COG5000">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019564_1_0_5"/>
    <dbReference type="OMA" id="SKFARMP"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017232">
        <property type="entry name" value="NtrY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045671">
        <property type="entry name" value="NtrY-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19312">
        <property type="entry name" value="NtrY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037532">
        <property type="entry name" value="STHK_NtrY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="11">
        <location>
            <begin position="315"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="495"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="713" mass="80847" checksum="426CD98ADE80E631" modified="2005-09-27" version="1">MKNWFIKLYNNISKFKFFRIIASFLAISVFLFSIITYCVVFHHDDPLGPDHSKAIKLVFFDLLLFMLLITSVSHRLINIWIQRKRGRLGSHLQTKIVLMFSVVAVIPTIIISGFSTLFFNYSIQAWFNKRVEAVMHESIQVAEAYLKEHKRNIRSDVLAISHYIAGHKVILNYDKVALQNIVQAKAELLGLAEVLIFQPGRVIANSKFSFTSSFDMIPWYDLDKIDNGKIVIVIKEDMIRAFLLLDGFSSTYVMVGRFIDRKVTSHLSATKGAVKSYRDLQSQISSLQIQFSMIFILISLLLLFTAIWYGINFAGDIVRPLLDLFYATRKVQKGDLSFKIEEGKVGEEMSTLARAFNQMVSQLNSQRSQLIKLYQDMNERREFIEAVLSGVSSGIIAVNCAGIITLMNEKARELLSPDDILYGELDDIFPEMSDLLKNSKMKDEIAVVRNKKSFTLSVRIKIFGSISKGYIITFDDISSLVDAQRSAAWSDVARRIAHEINNPITPIYLAAERLSSKYQNEIVSDKESFIRYIETIIRHVTSIRCIVDEFAKFAKMSDPVLVKHDICSVVKELSFSGQFSRGIVNYELNIPNQTIFVALDKSQINHVFINLFKNACESIDMKSDVQGLITISVIDGEGYVYVQIQDNGIGFPEELIDKLTEPYVTTRVQGTGLGLSIVKKILDEHNATISFYNIEGGGVVKLTFVKCNDHSDN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRD0</accession>
    <name>RL9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplI</name>
        <name type="ordered locus">Ecaj_0693</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304802.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRD0"/>
    <dbReference type="SMR" id="Q3YRD0"/>
    <dbReference type="STRING" id="269484.Ecaj_0693"/>
    <dbReference type="EnsemblBacteria" id="AAZ68725">
        <property type="protein sequence ID" value="AAZ68725"/>
        <property type="gene ID" value="Ecaj_0693"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0693"/>
    <dbReference type="eggNOG" id="COG0359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078938_1_1_5"/>
    <dbReference type="OMA" id="MKIILTH"/>
    <dbReference type="OrthoDB" id="1959318at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.430.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00503">
        <property type="entry name" value="Ribosomal_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_L9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_L9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020594">
        <property type="entry name" value="Ribosomal_L9_bac/chp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020069">
        <property type="entry name" value="Ribosomal_L9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036791">
        <property type="entry name" value="Ribosomal_L9_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_L9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_L9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="PTHR21368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03948">
        <property type="entry name" value="Ribosomal_L9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55653">
        <property type="entry name" value="SSF55653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="SSF55658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00158">
        <property type="entry name" value="L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000258454" description="50S ribosomal protein L9">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="208" mass="23420" checksum="72BD2179F820DCBE" modified="2005-09-27" version="1">MLSIILKESVRNLGKAGVVTKVKPGYARYLLTQKKAVRATKENLKNLEEQYLVIERENLEKLEAAKALKLSLEDEFLIITRQAADDGKLFGSVTPKCISKLLSDKGYNIHYRNIFFYSVIKYIGEYVVNLELHPDLVLPITLYVVKNDLGAMQAQKLHAEKKRKIEKEVEEGSGTSVDESLKLDSVSDSIDTSGVNSSDKEEENNIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRE8</accession>
    <name>Q3YRE8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">Pyruvate, phosphate dikinase</fullName>
            <ecNumber evidence="6 9">2.7.9.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ecaj_0675</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible phosphorylation of pyruvate and phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + phosphate + pyruvate = AMP + diphosphate + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10756"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.1" evidence="6 9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304784.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0675"/>
    <dbReference type="EnsemblBacteria" id="AAZ68707">
        <property type="protein sequence ID" value="AAZ68707"/>
        <property type="gene ID" value="Ecaj_0675"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0675"/>
    <dbReference type="eggNOG" id="COG0574">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015345_0_2_5"/>
    <dbReference type="OMA" id="RRFVQMY"/>
    <dbReference type="OrthoDB" id="997616at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050242">
        <property type="term" value="F:pyruvate, phosphate dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006090">
        <property type="term" value="P:pyruvate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010121">
        <property type="entry name" value="Pyruvate_phosphate_dikinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931">
        <property type="entry name" value="PTHR22931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000853">
        <property type="entry name" value="PPDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01828">
        <property type="entry name" value="pyru_phos_dikin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPDK_N" evidence="14">
        <location>
            <begin position="69"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="PPDK_N" evidence="14">
        <location>
            <begin position="305"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="13">
        <location>
            <begin position="424"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers_C" evidence="15">
        <location>
            <begin position="520"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="10">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="829"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="563"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="619"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="743"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="764"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="765"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="766"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAZ68707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="874" mass="97679" checksum="9A60944171002957" modified="2005-09-27" version="1">MSEKLIYYFSKTKCDGNLTLKHLLGGKGANLAEMCNIGVDVPPGFTLSTNLCNYYYQNNKLPDQVFSLVENAIEFLNNEVNLKFGDKDNPLLLSVRSGSVSSMPGMLDTILNVGLNDETVIGLTKKSGELFAYDSYRRLIQMYAHTVLQVENSLFEEVIYKKKRMYNIPINSQISDPNILKEIIAEFKKIVTFHTNTNFPQNVHKQLYNSIDAVFRSWMNKRAVAYRKIHNIPDNLGTAVNVQSMVFGNMSHNSATGVVFTRNPSTGAQEIFGEFLVNAQGEDVVSGTHSPLPLDKMKELMPQPYNDLEQVCKKLELHYRDMQDIEFTIENGKLWILQARTGKRSIQSSVKIAVDMVKENLISKEEAILRIDHKSFSKLFHPILENNTNINTIARGLPASPGAATGYVVFTPNDAEQFRKDGRNAILVRQETNPEDINGMNSAAGIITLRGGMTSHAAVVARGMGKPCICSATGIFIDKSEEFFYTSTGLKVHKGDSITINGCNGEVILGTIKTVIPKLSESFYTLMQWVDGIRTLKVMANADTPEDTDIAMSFQADGIGLCRTEHMFFSHNRISIVQEMIVSDNKEERIAALEKLEVMQKEDFKKIFKSTLNKQVTIRLLDPPLHEFLPDNNNAISEISARTGKSLEKLKNKILYLSEKNPMLGHRGCRLAISYPEIYEMQVRAIFLAVKELQEETGITCIVPEIMIPLVMAEKEIIIIKELINNIAQQFGNTQYSIGTMIELPKAALIADKIAKHVQFFSFGTNDLTQTTLGVSRDDSANFIGIYQNLEIVKQDPFETLDIEGVGKLISIAVDLGRKSYPNIKIGICGEHGGNFESIQFFSKLNLDYISCSAYRIPTARLIAAQSAIINQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRE9</accession>
    <name>Q3YRE9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RDD protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0674</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304783.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0674"/>
    <dbReference type="EnsemblBacteria" id="AAZ68706">
        <property type="protein sequence ID" value="AAZ68706"/>
        <property type="gene ID" value="Ecaj_0674"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0674"/>
    <dbReference type="eggNOG" id="COG1714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137439_0_0_5"/>
    <dbReference type="OMA" id="WYLVACF"/>
    <dbReference type="OrthoDB" id="1786723at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="6">
        <location>
            <begin position="8"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="163" mass="18536" checksum="C9E0B9A464B4FFB9" modified="2005-09-27" version="1">MNTKNIKVANILKRVLASTIDHIILSLLNYPVVLLTHELTIIPFIVNLLISCYYYTYFLSSTAQASIGQKILNIHTIRCDNKKIDACLAFDRSLSEFLCPTILMISIQIVNMNINNSFITSIIFLITIVTLIVSVVWYLIALFSQNNQTIHDIIFNTIVVENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRG5</accession>
    <name>Q3YRG5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="5">FGAM synthase</shortName>
            <ecNumber evidence="5">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="5">FGAR amidotransferase II</shortName>
            <shortName evidence="5">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">purL</name>
        <name evidence="9" type="ordered locus">Ecaj_0658</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FGAMS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304767.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0658"/>
    <dbReference type="EnsemblBacteria" id="AAZ68690">
        <property type="protein sequence ID" value="AAZ68690"/>
        <property type="gene ID" value="Ecaj_0658"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0658"/>
    <dbReference type="eggNOG" id="COG0046">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003100_0_0_5"/>
    <dbReference type="OMA" id="FIEPYQG"/>
    <dbReference type="OrthoDB" id="26038at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PTHR43555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGAR-AT_linker" evidence="8">
        <location>
            <begin position="222"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS" evidence="6">
        <location>
            <begin position="317"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="7">
        <location>
            <begin position="449"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS" evidence="6">
        <location>
            <begin position="715"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="7">
        <location>
            <begin position="830"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="555"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="485"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="745"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="782"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="785"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1004" mass="111642" checksum="993A46BDF13086CA" modified="2005-09-27" version="1">MIIRIGVIDKVLPEHYMRTNATVYTIDIKHHLNTETINKIGSLLTNPITQKFFAFIYDIRELNYKEITHNTNYNLLNTFKATWSLEKGFLPRVTDNVGNTTKCIINEALGNNSEVSVFTSQVIFGQEPIPQEDEVSKLFNSLIEYCKLSIAKGELFQVQFFGNKQILDLNKIYSNPSNSIFLIPYIKHEHLFPQQKAQNTYVTSVDLNIQDHKLIQISKHGINNRGPLNLSLEAMKAIQAYFKKIQRNPNDIELETLAQTWSEHCKHNIFSSPIDEITDGLYKHYIKRATQEINSEICVSTFSDNAGAIIFDDNFIIADKVETHNSPSALDPFGGAITGILGVNRDIIGFGKGAKPILNAYYFCFSESEEELYRDKSCTVKVLPSDVIMDGVIKGVNSGGNCSGIPTSLGSAYFDDRFRSKPLVFVGCTGIMPRTINNALSHLKKPNNGDFIVIAGGRTGRDGIHGATFSSNALTEHNSSSTVVQIGDPITQKKLSDAIIKEARDLDLYNAITDNGAGGLSSSVGEMGNNGFRVELDKVLLKHDNMLPWEIWVSESQERMTFAVPPNKFTMFEKVMKKHDVEISIIGEFNNTNKAVVLYKGSVIMDMDVDFLHNGVPKIHLQTNSYTQTIESIPNKKFNKPLAVELNEIIQRMNICSKEFISLQYDHEVQGTSVIKPLQGKGRVNGDATVIRPILSSNRGIVKSQGLGSSYGEISTYHMAACAIDTAIRNYVAVGGNFNHLALLDNFCWCDSTNPQRLWQLKNAAKACYDYAVAFKTPFISGKDSMFNDFKGYNKKGEEITISAPPSLLISTIGIIENVENAVTLDVKSPGDLIYILGITYNELGGSEYQKYTGIENNNVPQVHAEHAKKLYTSYNKAISANLIASAIAINLGGIIISLVKSLIGGQLGAEISLSSIPICNIENNHLQEKVILFSESQSRILVTVNENKKQEFETIFKNIPHANIGKVTSINTLIIDNMCSIDLNMLEYSYKKFSNMKIQSYAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRI5</accession>
    <name>Q3YRI5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304747.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68670">
        <property type="protein sequence ID" value="AAZ68670"/>
        <property type="gene ID" value="Ecaj_0636"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0636"/>
    <dbReference type="HOGENOM" id="CLU_2166989_0_0_5"/>
    <dbReference type="OMA" id="DQMFESV"/>
    <dbReference type="OrthoDB" id="1928311at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="98" mass="11207" checksum="87F8F89C53CA4F06" modified="2005-09-27" version="1">MADDEYKGVIQQYINTVKEIVSDSKTFDQMFESVVKIQERVMEANAQNDDGSQVKRIGSSTSDSISDSQYKELIEELKVIKKRLLRLEHKVLKPKEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRI6</accession>
    <name>Q3YRI6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Carbamoyl-phosphate synthase large chain</fullName>
            <ecNumber evidence="13">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Carbamoyl-phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">carB</name>
        <name evidence="17" type="ordered locus">Ecaj_0635</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 4 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="13"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304746.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0635"/>
    <dbReference type="EnsemblBacteria" id="AAZ68669">
        <property type="protein sequence ID" value="AAZ68669"/>
        <property type="gene ID" value="Ecaj_0635"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0635"/>
    <dbReference type="eggNOG" id="COG0458">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000513_1_0_5"/>
    <dbReference type="OMA" id="IEPAGIH"/>
    <dbReference type="OrthoDB" id="48855at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01424">
        <property type="entry name" value="MGS_CPS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01210_B">
        <property type="entry name" value="CPSase_L_chain_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CarbamoylP_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033937">
        <property type="entry name" value="MGS_CPS_CarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="SSF48108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="15">
        <location>
            <begin position="133"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="15">
        <location>
            <begin position="685"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="16">
        <location>
            <begin position="940"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carboxyphosphate synthetic domain" evidence="13">
        <location>
            <begin position="1"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate synthetic domain" evidence="13">
        <location>
            <begin position="557"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Allosteric domain" evidence="13">
        <location>
            <begin position="940"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="835"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="847"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="849"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAZ68669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1076" mass="118870" checksum="04AB640356EAC87A" modified="2005-09-27" version="1">MPKRTDIESILVIGAGPIVIGQACEFDYSGTQACKVLKQEGYRIILINSNPATIMTDPEIANSTYIEPITPSIIEKIIIKEKPSAILPTVGGQTALNVAIKLCEQGILEKHNVKLIGATKEAIQKAEDRNLFRQAMDKIGVKYPKSIIIKSVDEIKHALEYIGLPAIIRPSFTLGGIGGGISYNKEEFQKNVMYGLSISPISEVQVDESIIGWKEFEMEVMRDNKDNCIVVCSIENLDPMGVHTGDSITVAPALTLRDTEYQKMRDISFSILREIGVDTGGSNVQFAVNPKNDEDLLVIEMNPRVSRSSALASKATGYPIAKVAAKLAIGYSLDEIQNDCTKAIPASFEPSIDYIVTKIPRFNFDKFRGAEKELSTSMKSVGEVMAIGRSFPESLQKALCSLESGYSGLDEHFDKNIEKDQLYNALVKLSPDRIFIIADALRHNISIEEINKITGYDPWFLIQIKNIIQFEQQIKNHGLPKEAEGLLLLKKMGFSDARLAHLSNNTIEYVENLRRSLLINPVYKHIDTCAGEFKTNASYMYSCYEGDTVNPPECESVVTDNKKVIILGSGPNRIGQGIEFDYTCVHAAHTIRQMGYESIMINCNPETVSTDYDISDKLYFSPLTRESVLDIIFKEKETNSLLGVIVQFGGQTPLKLAKVLQEKNINILGTSFDSIDLAENRMKFQQLLTQLNLKQPPNVTCNSINEVYECVQSLAFPILARPSYVLGGQSMSIIRDSNALSNYLTIHKNIFDNGSLLLDQFLTNAVEVDVDAICDGESVYITGIMEHIEEAGIHSGDSACSLPAYTLTSEITDTIAEYTKKIALALQVIGFINIQYAIQDGEIYILEVNPRASRTVPFIAKATGIPIAKIATKILLGEKLPTILERKLNYIAVKEAVFSFSRFPNIDVLLGPEMKSTGEVMGIDKSFEIAFAKAQMAAGYELPTQGTAFISVKNSDKPLIVQTAQILQDIGFTIFSTKGTSTYLNEAGITTQQVNKVREGRPHILDLLQDDKINLVINTSECIKSFSESSSIRKTALVKKIPYSTTIPGAKALASAIKTLKKQGIQVEPMQEYTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRI8</accession>
    <name>Q3YRI8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydropteroate synthase</fullName>
            <shortName evidence="1">DHPS</shortName>
            <ecNumber evidence="1">2.5.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dihydropteroate pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DHPS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304744.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0633"/>
    <dbReference type="EnsemblBacteria" id="AAZ68667">
        <property type="protein sequence ID" value="AAZ68667"/>
        <property type="gene ID" value="Ecaj_0633"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0633"/>
    <dbReference type="eggNOG" id="COG0294">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008023_0_2_5"/>
    <dbReference type="OMA" id="FSIDTYH"/>
    <dbReference type="OrthoDB" id="1275854at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00156"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="PTHR20941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00792">
        <property type="entry name" value="DHPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="2">
        <location>
            <begin position="5"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="280" mass="31587" checksum="CB45DF73FD9222E9" modified="2005-09-27" version="1">MNLNTKLVGILNYTPDSFSDGGKFFSVDKALRQVEYLINGGADIIDVGGESTRPTYLYTQHGNNTISQTEEWNRLQDILPQIIEIAHYHNVKVSIDTRHAKTAEKAIELNVDYINDVSALSDDSMLQVLQSSSVDVIIMHSLGIPLIKNNTIPKDCDPINEIITWAQKHIEKLTNAGINKNRIIIDPGIGFSKTATQSLYILNNIDRLKILNQKIHVGHSRKFFLTSCKLNDLTRDNATLAVSIFLFQKGIDFIRIHNTHIHNDAFNILKKLQETNLNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRJ4</accession>
    <name>Q3YRJ4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ankyrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0627"/>
    <dbReference type="EnsemblBacteria" id="AAZ68661">
        <property type="protein sequence ID" value="AAZ68661"/>
        <property type="gene ID" value="Ecaj_0627"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0627"/>
    <dbReference type="eggNOG" id="COG0666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1832008_0_0_5"/>
    <dbReference type="OMA" id="ARAIMTM"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="SSF48403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="94"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="151" mass="17342" checksum="56B5C920A876B96B" modified="2005-09-27" version="1">MKDTSQEQKGDVKKSNISYKWPFTREVIEQVEKEKYDQMNEHLPKIYSVTDYYVQIFYCIIQNDISGLRSLVNKLQELDVSLTTILNKVRTLDVGDNLLTYAVKQKKIDVVRFLLSIGATPNFANYDGQTPLSIAIRNRSIDIAHAILAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRJ7</accession>
    <name>Q3YRJ7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AFG1-like ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304735.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0624"/>
    <dbReference type="EnsemblBacteria" id="AAZ68658">
        <property type="protein sequence ID" value="AAZ68658"/>
        <property type="gene ID" value="Ecaj_0624"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0624"/>
    <dbReference type="eggNOG" id="COG1485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008681_0_4_5"/>
    <dbReference type="OMA" id="FDEMQIT"/>
    <dbReference type="OrthoDB" id="607330at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="PTHR12169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="351" mass="41475" checksum="043AA940AE136311" modified="2005-09-27" version="1">MKEILNTYHSMVNDGKITYDEQQVQLLRKLVPYLSVEKICCFLPIKKKLRKIGIYIHGEVGRGKSMITDLYYDSCRIERKKRQHFNQFMKEVHTLLHEFKLSSVKDPLYKVAKVIVYNLDLLCLDEIQVYDICDAMILYRLFSIIFDQKVIVMMTSNYAPIELYQDGIQKQSFEPAISLIIDRMHVVHLSGEQDYRAVKELGVNDVYFIGDNSSAKLSDLFVKMANNRKICSVTLHVFGRDIEVRKVFNNTAWFDFYELCGKPLWVSDYQEIVRNFSIIFIAGIPVFDHYNQNEMRRFTILVDELYENRVKIFCALTVEPQLLYYLQEVPIDFQRTISRLMEMRSPSYYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRK3</accession>
    <name>Q3YRK3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal peptide peptidase A, Serine peptidase, MEROPS family S49</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0618</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S49 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304729.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0618"/>
    <dbReference type="EnsemblBacteria" id="AAZ68652">
        <property type="protein sequence ID" value="AAZ68652"/>
        <property type="gene ID" value="Ecaj_0618"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0618"/>
    <dbReference type="eggNOG" id="COG0616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046540_0_0_5"/>
    <dbReference type="OMA" id="YVYDEIR"/>
    <dbReference type="OrthoDB" id="1318587at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004635">
        <property type="entry name" value="Pept_S49_SppA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00706">
        <property type="entry name" value="SppA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S49" evidence="3">
        <location>
            <begin position="106"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="288" mass="32025" checksum="B6FAA6AF8C4024AA" modified="2005-09-27" version="1">MSDQLLKIEILNSKVKLWRFLVFLMVFASLILCNYVDISKVSNFLDNKYIAKVNIEGKIETSEEMDSLLKKIAKDSHVIGLILNINSPGGTITGSEILYQNIRNIAKNKPVVAVLNDLAASGGYMTAIAADYIIARHTTITGSIGVLMQYFGVSSLAEKMGISLKSIKSSNLKAATSPFEEFTKEKEESIRRIVNESYNYFIDIVADRRKMEKSQVLKIADGSIYTGSEALSIGLVDQIGGEDEAISWFQSQNINTQKVKVLSKKTHLSVLESINSLVLQVIHYYINK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRL0</accession>
    <name>RL4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplD</name>
        <name type="ordered locus">Ecaj_0611</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304723.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRL0"/>
    <dbReference type="SMR" id="Q3YRL0"/>
    <dbReference type="STRING" id="269484.Ecaj_0611"/>
    <dbReference type="EnsemblBacteria" id="AAZ68645">
        <property type="protein sequence ID" value="AAZ68645"/>
        <property type="gene ID" value="Ecaj_0611"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0611"/>
    <dbReference type="eggNOG" id="COG0088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041575_5_1_5"/>
    <dbReference type="OMA" id="PQVHILE"/>
    <dbReference type="OrthoDB" id="1572673at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_L4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4/L1e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="PTHR10746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000242370" description="50S ribosomal protein L4">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="205" mass="23180" checksum="8104FB5D89314D28" modified="2005-09-27" version="1">MEVSIINVNSDKIGTIDLNPLIFSVDYRPDILKMVVNWQLSKRRAGTHKAKTIGDISGTTAKPYRQKHTGRARQGSLRSPQFRGGAVIFGPVVRNHAYSLNKKVRKLGLKVALSLKNSCNKLLILDSIDVNFVKTTQVLQFIRNFEHKSFLIIDKDYNKNVVCSCRNLHNVTLLKQIGTNVLDILRHDCIILTVGAVKYLEERLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-11-25" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRL4</accession>
    <name>RL22_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplV</name>
        <name type="ordered locus">Ecaj_0607</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304719.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRL4"/>
    <dbReference type="SMR" id="Q3YRL4"/>
    <dbReference type="STRING" id="269484.Ecaj_0607"/>
    <dbReference type="EnsemblBacteria" id="AAZ68641">
        <property type="protein sequence ID" value="AAZ68641"/>
        <property type="gene ID" value="Ecaj_0607"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0607"/>
    <dbReference type="eggNOG" id="COG0091">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_3_0_5"/>
    <dbReference type="OMA" id="KRIQPRA"/>
    <dbReference type="OrthoDB" id="1666043at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_B">
        <property type="entry name" value="Ribosomal_L22_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_L22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_L22/L17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_L22/L17_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005727">
        <property type="entry name" value="Ribosomal_L22_bac/chlpt-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="PTHR13501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="SSF54843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01044">
        <property type="entry name" value="rplV_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000354464" description="50S ribosomal protein L22">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <sequence length="114" mass="12677" checksum="9122D3A9600597B2" modified="2005-09-27" version="1">MGKVLVEAKGIGLRSTPSKLNLVADLIRGKDVSVAMMYLKFCKKKSAGYISKVLKSAVANAQANYNVDLDNLYVKEVLVGKSFSLRRVHARARGKACRVHKHYGNVIIKLFERI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRM0</accession>
    <name>RL24_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplX</name>
        <name type="ordered locus">Ecaj_0601</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304713.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRM0"/>
    <dbReference type="SMR" id="Q3YRM0"/>
    <dbReference type="STRING" id="269484.Ecaj_0601"/>
    <dbReference type="EnsemblBacteria" id="AAZ68635">
        <property type="protein sequence ID" value="AAZ68635"/>
        <property type="gene ID" value="Ecaj_0601"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0601"/>
    <dbReference type="eggNOG" id="COG0198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093315_2_2_5"/>
    <dbReference type="OMA" id="HVKPTQE"/>
    <dbReference type="OrthoDB" id="2040741at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_B">
        <property type="entry name" value="Ribosomal_L24_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="KOW_RPL26/RPL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_L24/26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="PTHR12903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17136">
        <property type="entry name" value="ribosomal_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01079">
        <property type="entry name" value="rplX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000241595" description="50S ribosomal protein L24">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <sequence length="109" mass="11855" checksum="9D9158C351B97EE4" modified="2005-09-27" version="1">MGMKIVVGDDVVVISGKDKGKMGKVIKVLRKKHFGKDVSFAIVSGVNICKKSVKATQKSDGGIINIAKPINLSNIALFDSTLGIRTRVGYKFIDEKKVRFMKSSGKVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRN7</accession>
    <name>Q3YRN7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease P protein component</fullName>
            <shortName evidence="5">RNase P protein</shortName>
            <shortName evidence="5">RNaseP protein</shortName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein C5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rnpA</name>
        <name evidence="6" type="ordered locus">Ecaj_0584</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RnpA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304696.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0584"/>
    <dbReference type="EnsemblBacteria" id="AAZ68618">
        <property type="protein sequence ID" value="AAZ68618"/>
        <property type="gene ID" value="Ecaj_0584"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0584"/>
    <dbReference type="eggNOG" id="COG0594">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117179_6_3_5"/>
    <dbReference type="OMA" id="QHARKGY"/>
    <dbReference type="OrthoDB" id="2014742at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00227">
        <property type="entry name" value="RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000100">
        <property type="entry name" value="RNase_P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00825">
        <property type="entry name" value="Ribonuclease_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="119" mass="13686" checksum="958CC96B4BD2DFAC" modified="2005-09-27" version="1">MRLKGVTTLKKRKCFLFARNNGAFIGGFGLFLQVVKEPHGELLDGTIRVGFTVSKKVGKAVIRNKVKRRFRILARDVLVKHANKGYYYILIGNRYTPKVSFKEMSLRLISYLNTSRLYS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRP5</accession>
    <name>RL32_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmF</name>
        <name type="ordered locus">Ecaj_0576</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304688.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRP5"/>
    <dbReference type="SMR" id="Q3YRP5"/>
    <dbReference type="STRING" id="269484.Ecaj_0576"/>
    <dbReference type="EnsemblBacteria" id="AAZ68610">
        <property type="protein sequence ID" value="AAZ68610"/>
        <property type="gene ID" value="Ecaj_0576"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0576"/>
    <dbReference type="eggNOG" id="COG0333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129084_2_0_5"/>
    <dbReference type="OMA" id="GMHRAHD"/>
    <dbReference type="OrthoDB" id="2092354at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00340">
        <property type="entry name" value="Ribosomal_L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_L32p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044957">
        <property type="entry name" value="Ribosomal_L32p_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35534">
        <property type="entry name" value="PTHR35534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01031">
        <property type="entry name" value="rpmF_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000225721" description="50S ribosomal protein L32">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00340"/>
        </source>
    </evidence>
    <sequence length="61" mass="7024" checksum="9A815E39337C1B46" modified="2005-09-27" version="1">MAVPKRKKSKSRRNMHRSHCRLRVPNIGIDKTTGEYKLSHHICLGGYYNEKQVLEVDTSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRS3</accession>
    <name>Q3YRS3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidase M24</fullName>
            <ecNumber evidence="5">3.4.11.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0547</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304660.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0547"/>
    <dbReference type="EnsemblBacteria" id="AAZ68582">
        <property type="protein sequence ID" value="AAZ68582"/>
        <property type="gene ID" value="Ecaj_0547"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0547"/>
    <dbReference type="eggNOG" id="COG0006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011781_2_1_5"/>
    <dbReference type="OMA" id="YRPGKWG"/>
    <dbReference type="OrthoDB" id="415910at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="3">
        <location>
            <begin position="4"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="2">
        <location>
            <begin position="320"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24_C" evidence="4">
        <location>
            <begin position="511"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="574" mass="65358" checksum="F5FB6BFCC5CC4EC5" modified="2005-09-27" version="1">MKSRLNQLINLMSEYAIDLLLLHNTDEYQSEYVPVNKQRVKWLCGFSGSNATLIISREGKQHFFTDGRYILQATRELDLNDYQIHNVCDLTPWQWCVENLASNAVIAYESSLVTLSHVKKYEDCSLILRPLDQILIDKLWIRDFSMECDIVEHPLKYSGVESYKKSCEAVKYLSGKDAALITDTDVISWLLNIRNNKFVYNPSVLSRAILYSDGRVDLFVDDICSVDVKYEHLKVYSLNDLFNVLESIESIVIDASTIPMNVFQCLNQQDILIKNSDNCLLMKSEKNNVEIQGAVNAHIRDGVAVINLLYWLNVQLDNNQEITELDVESKLLSFRQEQDLFRGESFATISGFGENGAIIHYRANNNTNKLICKNGLYLLDSGGQYLDGTTDITRTIVVGEPTPEQITNFTLVLKGHIALATAVFPLGTNGGMLEVLARQYLWKSGLDYQHGTGHGVGSFLSVHEGPCAISCRNDIVLKPNMVLSNEPGYYKNGEYGIRIENLMYVEKCMDNFLRFKQLTCVPIDLKLIDSNMLNNEEISYIDQYHSFVYNTVAPYLDQKVKCWLHNACRNLKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRS5</accession>
    <name>Q3YRS5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GATase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304658.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0545"/>
    <dbReference type="EnsemblBacteria" id="AAZ68580">
        <property type="protein sequence ID" value="AAZ68580"/>
        <property type="gene ID" value="Ecaj_0545"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0545"/>
    <dbReference type="eggNOG" id="COG2071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_865285_0_0_5"/>
    <dbReference type="OMA" id="PGNYYNI"/>
    <dbReference type="OrthoDB" id="1503348at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase" evidence="1">
        <location>
            <begin position="98"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="324" mass="36778" checksum="143DF91690A37498" modified="2005-09-27" version="1">MKFTFRINYTVILTLFFLTFAIPYVTVLAGCVKYNKKDIVVGLLSTEFVSYPNEYNTALGITKLLESYGVHVVLMDYNVIMQLVQDEIISINKTYEKSYEISRYDIIKNVVMKFIKENNISRILIPGNHYNISSLPIAPSLNRQLITNAVVSIIHNDNHCIHLFGVCGGLQGIMYANGIRVTRVENIIKSQISVKSHTVSAPNPRNVDANLHQIKILPNNRLSDIAASLDLLAKNQVIYFPDSHSEAIDTDIENIKKIYNLGYKIIAMSEDGIIEGIEDNYGNMYFQFHPEYLMINAEKKRGDNNREASIALADAIIKDFLFRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRT2</accession>
    <name>LIPA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipoyl synthase</fullName>
            <ecNumber evidence="1">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lip-syn</fullName>
            <shortName evidence="1">LS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Sulfur insertion protein LipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lipA</name>
        <name type="ordered locus">Ecaj_0537</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304651.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRT2"/>
    <dbReference type="SMR" id="Q3YRT2"/>
    <dbReference type="STRING" id="269484.Ecaj_0537"/>
    <dbReference type="EnsemblBacteria" id="AAZ68573">
        <property type="protein sequence ID" value="AAZ68573"/>
        <property type="gene ID" value="Ecaj_0537"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0537"/>
    <dbReference type="eggNOG" id="COG0320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033144_2_1_5"/>
    <dbReference type="OMA" id="PYCDIDF"/>
    <dbReference type="OrthoDB" id="1184806at2"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102552">
        <property type="term" value="F:lipoyl synthase activity (acting on glycine-cleavage complex H protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102553">
        <property type="term" value="F:lipoyl synthase activity (acting on pyruvate dehydrogenase E2 protein)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031691">
        <property type="entry name" value="LIAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="PTHR10949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16881">
        <property type="entry name" value="LIAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005963">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000012216" description="Lipoyl synthase">
        <location>
            <begin position="1"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="46"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="299" mass="33985" checksum="3A20E3DE31B2856F" modified="2005-09-27" version="1">MRSKPDWLKVKMPTGDTFYQMRNLMKLYKLNTVCEEAACPNVGECWNKKHATVMILGSTCTRACAFCNVATGIPDKLDPHEPQNLAKAINSLKLHHVVITSVDRDDLPDGGAGHFVECIEEIRKRDDNITIEILTPDFLNKHGAIDKIAAVAPDVYNHNVETVPRLYARIRPKARYFHSLYLLKAVKYKNPKIFTKSGIMVGLGETKEEIYQVMDDLRSADVDFITIGQYLQPTPKHAVVDRYVTPEEFDHYKYVAYSKGFLMVASGPLVRSSYHAEEDFQKLKQNRTAMLMRAESNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRT3</accession>
    <name>Q3YRT3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphate ABC transporter membrane protein 2, PhoT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0536</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="Ecaj_0536"/>
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    <dbReference type="KEGG" id="ecn:Ecaj_0536"/>
    <dbReference type="eggNOG" id="COG0581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_2_1_5"/>
    <dbReference type="OMA" id="GIMSGIC"/>
    <dbReference type="OrthoDB" id="1763210at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:transmembrane transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024573">
        <property type="entry name" value="DUF3333"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11812">
        <property type="entry name" value="DUF3333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="387"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="202"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="421" mass="46776" checksum="DA8BD8B1AA189558" modified="2005-09-27" version="1">MYIKKKLGKLLKYRHIASRISLKNRKDRILSTLSMSALISSLGILFIILGSIVNNGYNALTTTTILLSIKINDNIEQQDKLILRSNLINYIHQSLEELFSDVIDTSNLDNKKIIYSIVSDTAHHNLADFVFKNPKANKFNMWFKSSTTFNKIIQTQSTDNTIYKKIITKLQNEHRIKKSFNDTLFKKYNSISPENTGIIGSLIGSMFTIIICLAFAFPIGITSGICLSELIPKNKISSIIEISITNLASVPSIIFGILGLAIYIKIFNIPRSSALIGGMTLSLMMLPNLVIATKQAFTAVPSSVKDAAFSLGASNMQVILHHSFPIALPGIMQGAILSIARILGESSPLIMIGMVAFIVDIPKNFLDPTTVLPVQIYMWASNPHIEFIQLAAIAIVALLLMLLILNLIVTFIRKKFDHFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRT7</accession>
    <name>Q3YRT7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TrbL/VirB6 plasmid conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0532</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TrbL/VirB6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0532"/>
    <dbReference type="EnsemblBacteria" id="AAZ68568">
        <property type="protein sequence ID" value="AAZ68568"/>
        <property type="gene ID" value="Ecaj_0532"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0532"/>
    <dbReference type="eggNOG" id="COG3704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_344442_0_0_5"/>
    <dbReference type="OMA" id="PWDRLDC"/>
    <dbReference type="OrthoDB" id="200146at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030255">
        <property type="term" value="P:protein secretion by the type IV secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007688">
        <property type="entry name" value="Conjugal_tfr_TrbL/VirB6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04610">
        <property type="entry name" value="TrbL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="552"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="578"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="611"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="706"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="779"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="779"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="839" mass="91176" checksum="C3EB3199CBBFCAB0" modified="2005-09-27" version="1">MLKISKGIVYILMLFLININFIYAKSTLATEVESEKVATYRTTLATNPFCSRVSEHAKYIGISAFAVGFGLIITAIVMAVTGVGAIGALLVFGLGVGAIAVAVNKAAAFFACHWSFVRHPVMRFESSKGGANPGDYKECSEPTTSYKSCADRDFGSEEEYFRCLATDDAKSTKPFSLAEAKQNEPVCASKNFKKVDKYYWPKNRVSSSRYIEVCYRKPIGGMNIASMIDRASKANSGDVVKLLKEGDYPREGDYSTRDIQVAKYAMNGRVECAVLEAGQEKVIHSITFQAVEKMDKICVNAVKAFGSALWPQVEIGCHMRPSGPPVPMCEKSEPVMSEDNKRIISYDNTKCYSCYIDPACRGEVGGYVKSVFPMTSMIVSCVKGSLNNVLTGQCSNGVNTGKAGFLKVAQDRLKRTVMAVLVLSLILFAIKAALGGIQSPAELYMLIIKFGLVVYFTQGPAMSHYYDQIVRLSIGLSDIVLQAGGSQTVCNYTDQEYKDPYKYVAPWDRLDCRIMFYLGQQLNGAATTIVLGLFLTAGLFFASMLFNMKLMLCLVALFAILMIILIVIWLVYIFLLSLIALTILILISPLMIPMVLFQATKAFFDGWIRQLMVYTLYPVILFAFLALLFTVFDNLYFEDLKFVRKEIKILDATRISFTLENPKQCDDPKYDYNLACMFGDIRFLTKPAFFGFNAYAPDFKHEAETLWMKLLMMVFIGFLFYHFLSSITYIAAELAGDPRAGHVGSSFSPKSMMSSITGAIGRVQGNMGQKVKDAINDRMDNAKGSKDGGDKDEMSGGDSGGGAAGGGGAAGGASAAGAAGGAASGAASGAAGAIASAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRU0</accession>
    <name>Q3YRU0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Putative Type IV secretory pathway VirB6 component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0529</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TrbL/VirB6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304643.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0529"/>
    <dbReference type="EnsemblBacteria" id="AAZ68565">
        <property type="protein sequence ID" value="AAZ68565"/>
        <property type="gene ID" value="Ecaj_0529"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0529"/>
    <dbReference type="eggNOG" id="COG1511">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000715_0_0_5"/>
    <dbReference type="OrthoDB" id="62584at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030255">
        <property type="term" value="P:protein secretion by the type IV secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007688">
        <property type="entry name" value="Conjugal_tfr_TrbL/VirB6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04610">
        <property type="entry name" value="TrbL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230977" evidence="11">
        <location>
            <begin position="23"/>
            <end position="2030"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="439"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="924"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1087"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1184"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1241"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1564"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1695"/>
            <end position="1933"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1979"/>
            <end position="2029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="939"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1190"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1246"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1302"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1358"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1418"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1474"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1603"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1719"/>
            <end position="1740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1770"/>
            <end position="1838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1879"/>
            <end position="1896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1905"/>
            <end position="1922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="2030" mass="220181" checksum="DD4A0B35948157E8" modified="2005-09-27" version="1">MCNFIKKHFVKLLLLLVITACESNQHPVPRCIPADVLSETKTASVSAYFNASSEDFIADDKSLGNILNNDQVVRWKYTGYVTDGNPIVLRVDGMWTAWADNASESDRGVTDYEKYNNILSSERICGPYNKIQKTFVPYGKSGCKVSCELITGVQDELERGAYGPPCWFKNGYGAYLLFKRPGDPEPNETLNHMRYPVSPVMHIGYKPLELAGSDALSTRDRKIMDSFCQEVKLEPGWKIYLKILDKHYYDNVGGYSVTFVEGIKAEERFSVFEWVRKNIRNELDKAGEHVFKHIVGNPVFKNFVFALLTLFLVFGALTYIFGMVQSPFSDVIVRILKMALMILLISPSSWEFFYNHLLNLFINGIDQIIAMINSHSASYNPTRPFAFLDEMIVDKIFSPVIWKIKIRALILADFSSIFAVLVIIIAVLIYIALCLYGCVIYLTAFVGITFLIGLFPLLLLGILFSQFKSLFDGWLTQCLSFSMQAILMFTLISLFGALIMNYYYRIFGFTTCYNEWLKVKVCILGSTGCVINRSVFGWTPGQIYDPKVIGLTTDFNLNDRKTSDNARYKFTGGGAYISVPPERIHKDFRYVDYPFLDPDSQTDGDPFGITVSKDSPFRQLSYYINALLASNEKYVAARLIKDIEDELEQLQKNGTINSDSRNKVLTIINNRRNTDKSQQKNALDKYQDEDFKSEIINSLINDVIGEAAQAPTPQDELMKQHDYNLILGIKAGYLVLWSEVGSLFLAALLIWQMRAFIQSVAVALAGGSMMSQTIANMYEGGFIKIFSGIPVLGVVFKKIDQGIDSLKFVTGNYITKTARRPLEALKRVPYLGKVVKFTSAATAPLTSSYNEYDRDFRNNFKTLNYARAYIGAHLGISPLNAMKYLGGHVAGSMFGTREGGLIHNILEDRAAALDSLKAHILGPEQYKPSPYLPNAGEDDDRNPFAKEKPHDAKETPHGNGDNRLLDENGNISVNRDNLSDALDAREQLKTMIGNTTDEAALQRMRYDLDRLDDALHKNLGNDFDRVTSAYTNSYTPVEHPKDLLVEGMSGLRVSSENLQGLGVPTVDELVQADNSLNVPLSSDVISSGTQLHGSSGAHLDVTPPVSDVDSLLSQQDASDSARVGGFGESISTSEHGVVVEGIHDSVLRDNAGLTTPLDVTSSMESVGAGGDDAARAPVEVFEDPAGTPVESQDFGSTLDDSNVTQSSSGESDLGSTSSMEGVGADGDDAARPAVEVFEDPAGTPVESQDFGSTLDDSNVTQSSSGESDLGSTSSMESVGADGDDAARAPVEVFEDPAGTPVESQDFGSTLDDSNITQSSSGESDLGSTSSMESVGADGDDAARAPVEVFEDPAGTPVESQDFGSTLDDSNVTESSSGESDLGSTSSLESVGADGDDAARAPVEVFEDPAGAPVESQDFGSTLDDSNVTESSSGESDLGSTSSLESVGADGDDAARAPVEVFEDPAGAPVESQDFGSTLDDSNVTESSSGESDLGSTSSMEGVGADGDDAARAAAEILNEPTSIPEDSDVFEKVELKDLSPALDDVHGLDDDITQAHDNAVEDHFGPLEEGNTMFSQDAHPDQNVDSGLEEETALHSGIMKQDEMDTSESTVQTDTTTPEDNIVQETDDSINLDVGVFEQTSGVLDLGSLENDVNIVGNEGILQQQAAESLLDDVPKPEIDDGTVLEKVGDEVIQGEESVFEKSGIQETEPYSTAMEQVTDKDVGRDGDAGTEKLFTTEDDTADTKVGPSVELTSEGVVNEAVQDGFSSDNEGKLSEETKDDLVTQQEDKLEDKGPDDTDDVQGEDKQYQDVVDEDNIVEKTGDVSDQSDGKEDAESGVSDSEALAEGLIDLVSSGEESTQAQRIQVIHPVAGLSSRDKGVVTQDKSGSSKGSVGGKGIFSAQSDVKGEVKEKKKEPSKSKAKSPSKTKSPFITPKSLKNTFKFVMDECAKDFSSKVLESMDKIFGESQGGKQRRRKLSKEEISLAIEQLEAVIEGLKLKKAGLTDPSEIEAIEIRIKEAESAIRGLLNQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRU6</accession>
    <name>GPMI_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="1">BPG-independent PGAM</shortName>
            <shortName evidence="1">Phosphoglyceromutase</shortName>
            <shortName evidence="1">iPGM</shortName>
            <ecNumber evidence="1">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gpmI</name>
        <name type="ordered locus">Ecaj_0523</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BPG-independent phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304637.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRU6"/>
    <dbReference type="SMR" id="Q3YRU6"/>
    <dbReference type="STRING" id="269484.Ecaj_0523"/>
    <dbReference type="PRIDE" id="Q3YRU6"/>
    <dbReference type="EnsemblBacteria" id="AAZ68559">
        <property type="protein sequence ID" value="AAZ68559"/>
        <property type="gene ID" value="Ecaj_0523"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0523"/>
    <dbReference type="eggNOG" id="COG0696">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026099_2_0_5"/>
    <dbReference type="OMA" id="FMDGRDT"/>
    <dbReference type="OrthoDB" id="338375at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046537">
        <property type="term" value="F:2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006007">
        <property type="term" value="P:glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16010">
        <property type="entry name" value="iPGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01038">
        <property type="entry name" value="GpmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011258">
        <property type="entry name" value="BPG-indep_PGM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036646">
        <property type="entry name" value="PGAM_B_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005995">
        <property type="entry name" value="Pgm_bpd_ind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31637">
        <property type="entry name" value="PTHR31637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06415">
        <property type="entry name" value="iPGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001492">
        <property type="entry name" value="IPGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64158">
        <property type="entry name" value="SSF64158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01307">
        <property type="entry name" value="pgm_bpd_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000212142" description="2,3-bisphosphoglycerate-independent phosphoglycerate mutase">
        <location>
            <begin position="1"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="1">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="150"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="253"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01038"/>
        </source>
    </evidence>
    <sequence length="501" mass="56125" checksum="03B412D30D1DD9AB" modified="2005-09-27" version="1">MTNHSVILCILDGWGNGENNQFNAIAQAYKPCWDNITLQYPKSHLITHGSSVGLPDGQIGNSEVGHVNLGSGRIVLQDLCKINNEIKYIKDNVYLLEFINKIKKNNGICHIAGLLSDGGVHSSYTHILDIINTLSDFQIQVAVHIFLDGRDTPPISATKYISILCEHIKHLNNINIATLSGRYYAMDRDNRLDRTTKAYNAIAFANAKRYEDPLTAVQDSYNLGITDEFILPCIIGNYQGMKPQDGFIMTNFRSDRVIQIIKMIIGDIKTDNHITLDNTIGMVKYSDKINIPCLFPNNNITNTLGEVIANNQLHQLRIAETEKYAHVTFFFNGGKEDMFENEDRIIIPSPSVATYNLTPEMSAEKVTDTIIEKIKLQKYSLIIVNYANADMVGHTGNIDATKQAITTIDQCLSKILNCIQNTNYVLVITSDHGNAEEMFDVKNNMPYTAHTLNPVPFIICNYNKKIRLKNGRLCDVAPTILEILNLAKPKEMTGVSLIEQV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRV1</accession>
    <name>NTPP_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside triphosphate pyrophosphatase</fullName>
            <ecNumber evidence="1">3.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleotide pyrophosphatase</fullName>
            <shortName evidence="1">Nucleotide PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">Ecaj_0518</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleoside triphosphate pyrophosphatase. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-phosphate + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23996"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside 5'-phosphate + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44644"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Maf family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304632.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRV1"/>
    <dbReference type="SMR" id="Q3YRV1"/>
    <dbReference type="STRING" id="269484.Ecaj_0518"/>
    <dbReference type="EnsemblBacteria" id="AAZ68554">
        <property type="protein sequence ID" value="AAZ68554"/>
        <property type="gene ID" value="Ecaj_0518"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0518"/>
    <dbReference type="eggNOG" id="COG0424">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040416_2_0_5"/>
    <dbReference type="OMA" id="RIDGDFY"/>
    <dbReference type="OrthoDB" id="1469203at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036218">
        <property type="term" value="F:dTTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="PTHR43213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000267302" description="Nucleoside triphosphate pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00528"/>
        </source>
    </evidence>
    <sequence length="192" mass="21637" checksum="AF52C6A40A59E1CF" modified="2005-09-27" version="1">MLKFDNLILASSSKQRLSLLEQIGVVPGEIISPDIDEVILKKELPKAYSIRIAQAKCEKVKLLHPDKFVLSADTVVCCGRRILPKVETEEQALECIRLISGRRHRVYTTVCLYTPHGKLHCRNVVTVVKFKNLSVQEIDAYIDSRQWYGKSGACSIQTNAGKFVLSINGSYSSIIGLPLYETYSILNRYFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRV8</accession>
    <name>Q3YRV8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Cytochrome b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Ecaj_0511</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15">Binds 2 heme groups non-covalently.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="14">Binds 2 heme groups non-covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the cytochrome b family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304625.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0511"/>
    <dbReference type="EnsemblBacteria" id="AAZ68547">
        <property type="protein sequence ID" value="AAZ68547"/>
        <property type="gene ID" value="Ecaj_0511"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0511"/>
    <dbReference type="eggNOG" id="COG1290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031114_3_0_5"/>
    <dbReference type="OMA" id="RFFAFHF"/>
    <dbReference type="OrthoDB" id="262439at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:ubiquinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00290">
        <property type="entry name" value="cytochrome_b_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00284">
        <property type="entry name" value="Cytochrome_b_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005798">
        <property type="entry name" value="Cyt_b/b6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036150">
        <property type="entry name" value="Cyt_b/b6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005797">
        <property type="entry name" value="Cyt_b/b6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030689">
        <property type="entry name" value="Cytochrome_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00032">
        <property type="entry name" value="Cytochrom_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00033">
        <property type="entry name" value="Cytochrome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038885">
        <property type="entry name" value="COB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="SSF81342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81648">
        <property type="entry name" value="SSF81648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51003">
        <property type="entry name" value="CYTB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51002">
        <property type="entry name" value="CYTB_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="238"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="CYTB_NTER" evidence="17">
        <location>
            <begin position="15"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="CYTB_CTER" evidence="18">
        <location>
            <begin position="228"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>b562</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>b566</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>b562</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>b566</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038885-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038885-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AAZ68547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="408" mass="46894" checksum="79AE44127B8D1CD4" modified="2005-09-27" version="1">MSGHDNIKKPEGKGILAWIEYRMPICAFLKGLASYQVPKNLNYAWNFGSLAGIALILQIITGIFLAMHYTPHVAHAFNSVERIMRDVNYGWLIRYTHAVGASFFFIVVYIHILRGLYYGSYKSPRELVWFIGIFIFFAMMATAFMGYVLPWGQMSFWGATVITNLFSVIPLVGQDIVQWLWGGFSVDNPTLNRFFALHYLLPFIILALASLHVIALHRFGSGNPSGVEVKSKKDTIPIYPYFIVKDCITFGIFFIFLFLFVFYIPNYLGHPDNYIEADPMVTPAHIVPEWYFLPFYAMLRSIPNKLLGVVTMIGSIMVWFLLPILDRCKVKSGSYRPIFRIFYVLFVLNFCFLTWLGGQEVKEPFVTLSRLSTLYYFSYFLIVLPILSKYEKPVTLPNTVSDAVPEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRX4</accession>
    <name>Q3YRX4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0494</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304609.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0494"/>
    <dbReference type="EnsemblBacteria" id="AAZ68531">
        <property type="protein sequence ID" value="AAZ68531"/>
        <property type="gene ID" value="Ecaj_0494"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0494"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_1_5"/>
    <dbReference type="OMA" id="MNTHADR"/>
    <dbReference type="OrthoDB" id="1996723at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="3"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="4">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="147" mass="16966" checksum="CC0F7F9B0253F952" modified="2005-09-27" version="1">MTVEIGDDAPYFKSLDTYKGDKNIILYFYPKDDTPGCTKEAKDFQNAMDDFVDLNTVIIGVSKDNEKSHDNFKRKHNILFELISDEGSELSKQYGTWVEKNMFGKAYMGIGRSTFLIDKNGKIRKIWRNVKVSGHVDNILEIIREIM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRY1</accession>
    <name>RL27_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmA</name>
        <name type="ordered locus">Ecaj_0487</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304602.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRY1"/>
    <dbReference type="SMR" id="Q3YRY1"/>
    <dbReference type="STRING" id="269484.Ecaj_0487"/>
    <dbReference type="EnsemblBacteria" id="AAZ68524">
        <property type="protein sequence ID" value="AAZ68524"/>
        <property type="gene ID" value="Ecaj_0487"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0487"/>
    <dbReference type="eggNOG" id="COG0211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095424_4_1_5"/>
    <dbReference type="OMA" id="GKDHTLH"/>
    <dbReference type="OrthoDB" id="1904463at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00539">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_L27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_L27_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="PTHR15893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000017472" description="50S ribosomal protein L27">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="88" mass="9580" checksum="F1643F9DF75D57AD" modified="2005-09-27" version="1">MATKKSGGSSGNGRDSRGRRLGVKKFGSEKVIPGNIIIRQRGTKYHPGKNVGMGKDHTIFSKISGFVHFRKGVFNKTFVDVLEISSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRY9</accession>
    <name>Q3YRY9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0479</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304594.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68516">
        <property type="protein sequence ID" value="AAZ68516"/>
        <property type="gene ID" value="Ecaj_0479"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0479"/>
    <dbReference type="HOGENOM" id="CLU_118039_0_0_5"/>
    <dbReference type="OMA" id="REYSTTH"/>
    <dbReference type="OrthoDB" id="2010142at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="193" mass="21985" checksum="A13075D28FB409D3" modified="2005-09-27" version="1">MELILLFEGNVPVLYPASVCTYNKLQDSYTISYPTLDISTTVQRPGSDVPLYRLYGSKFNSYCIVSNTSLPRIMYYLYGTTRGGGEDSPFLIFGLKHNSLSRLGEEGVVLDDEVLLNGGFLRDSGTVEENTVQLFERFRRFMKLESDVGTPRTYRFDFFNSGGDLFHTDYRSTQLEQVAVNPVTGNNRYIMHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS12</accession>
    <name>Q3YS12_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304571.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0456"/>
    <dbReference type="EnsemblBacteria" id="AAZ68493">
        <property type="protein sequence ID" value="AAZ68493"/>
        <property type="gene ID" value="Ecaj_0456"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0456"/>
    <dbReference type="HOGENOM" id="CLU_1685864_0_0_5"/>
    <dbReference type="OMA" id="KKEMFLA"/>
    <dbReference type="OrthoDB" id="1867372at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="153" mass="17272" checksum="CD7DD3CFBADEFDB8" modified="2005-09-27" version="1">MGDEINKDSKFAKQFASNRKGGTGSFTDRYKNPNALPNKSAKFSGMLQENKSEKEVPFSDSKSVLRKSKLNNKNFAESTKKSRLDCLFLVRGKDNGRAAWHYVLVDKHKREMFLAKSRSGSIDVALYGQILYSGWGENPPEDIVKKIEDEFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS17</accession>
    <name>Q3YS17_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Type IV secretion system protein VirB8, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0448</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304566.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0448"/>
    <dbReference type="EnsemblBacteria" id="AAZ68488">
        <property type="protein sequence ID" value="AAZ68488"/>
        <property type="gene ID" value="Ecaj_0448"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0448"/>
    <dbReference type="eggNOG" id="COG3736">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105348_0_0_5"/>
    <dbReference type="OMA" id="TSIVYMQ"/>
    <dbReference type="OrthoDB" id="1477189at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007430">
        <property type="entry name" value="VirB8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04335">
        <property type="entry name" value="VirB8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="VirB8" evidence="6">
        <location>
            <begin position="32"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="227" mass="26579" checksum="91F08E10DAA552A2" modified="2005-09-27" version="1">MDHIQNNNRYIGSSKYFKDAFDWYCNRYLLCVTERAWLLVISLFLSSMLLILILDIFAYFPLKTDFSFVKYTERYTDEFSVIKKLSTNNEDSEETLLSQYLVAQYVKRYESYLHKDLDVQFNFVKNNSSRKIYLAFKEMVDSNHYTDAKYKTMSIEATINNVKLLSKDFTSLNSAIVTFQTRVSNNGIASHVKSHKVLVTFSLSSIKMAISGIMPLEFVVYDYKKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS51</accession>
    <name>Q3YS51_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, BadM/Rrf2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0411</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304532.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0411"/>
    <dbReference type="EnsemblBacteria" id="AAZ68454">
        <property type="protein sequence ID" value="AAZ68454"/>
        <property type="gene ID" value="Ecaj_0411"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0411"/>
    <dbReference type="eggNOG" id="COG1104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_0_0_5"/>
    <dbReference type="OrthoDB" id="839689at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030489">
        <property type="entry name" value="TR_Rrf2-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00738">
        <property type="entry name" value="rrf2_super"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01332">
        <property type="entry name" value="HTH_RRF2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="4">
        <location>
            <begin position="164"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="533" mass="59400" checksum="FB87D6950FFEFFA9" modified="2005-09-27" version="1">MLITTRLRYAVMFMVSLAQEYYILKGSNQPKKMSHIADSQSLSEGYLEQIIAKLKKQGLVNSTKGPGGGYFLNKSPNIITLNLILESIGENIKITRCKNNNVIRCLPNNAKCITHNLWDNIGNYIKKYLNSISLEDIINNNFKHSIKHSIKHSNILNNDENKYIYADYNSTSTILPVIKQQLDNLSSLNIYNPSSTHKLGQNTRSIIEKTREIAINILNARNYDVIFTSSGTEANNLVINSTTEYKHLISSIEHVSIMNCTTDAELIPVNSNGVICLDRFSETLNKFKDKKVLVSIMTANNETGVIQPIKEIVEISHKFGALVHTDAVQACGKIHIDIENLGVDLLTISSHKLGSIAGTGVLFFNSKKIKIKPMILGGNQEKGLRAGTENIISIYLLSISLNNLKDSIKKMSTVEKLRDKLEYEILNLVPEAQIFGKNTQRLPNTTCISMPNVSSEIQTISFDIENIAVGNGSACSSGTLTRSHVLSAMGIDDDIAKNSIRISLSPDITEHQIKKIVNCWYKTYQNNKLLKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS57</accession>
    <name>Q3YS57_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2Fe-2S ferredoxin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Adrenodoxin-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0405</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304526.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0405"/>
    <dbReference type="EnsemblBacteria" id="AAZ68448">
        <property type="protein sequence ID" value="AAZ68448"/>
        <property type="gene ID" value="Ecaj_0405"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0405"/>
    <dbReference type="eggNOG" id="COG0633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_5_0_5"/>
    <dbReference type="OMA" id="SACGGVC"/>
    <dbReference type="OrthoDB" id="1837979at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="PTHR23426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="6">
        <location>
            <begin position="2"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="116" mass="12895" checksum="3D136A4AA035915A" modified="2005-09-27" version="1">MPLITFISPDGSRKTYEAYDGETLLSLAHRNNVDLEGACEGSLACSTCHVIIDPSWYDIVEKHNEISDEENDMLDLAFGLTDTSRLGCQIILTKELDGLCVILPTETRNISFIKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS60</accession>
    <name>Q3YS60_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinone biosynthesis O-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-polyprenyl-6-hydroxyphenol methylase</fullName>
            <ecNumber evidence="1">2.1.1.222</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">3-demethylubiquinone 3-O-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.64</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiG</name>
        <name evidence="2" type="ordered locus">Ecaj_0402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3-(all-trans-polyprenyl)benzene-1,2-diol + S-adenosyl-L-methionine = a 2-methoxy-6-(all-trans-polyprenyl)phenol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:31411"/>
            <dbReference type="Rhea" id="RHEA-COMP:9550"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:62729"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="EC" id="2.1.1.222"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:44380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="2.1.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. UbiG/COQ3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.222" evidence="1"/>
    <dbReference type="EC" id="2.1.1.64" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304523.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0402"/>
    <dbReference type="EnsemblBacteria" id="AAZ68445">
        <property type="protein sequence ID" value="AAZ68445"/>
        <property type="gene ID" value="Ecaj_0402"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0402"/>
    <dbReference type="eggNOG" id="COG2227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042432_0_0_5"/>
    <dbReference type="OMA" id="RGTHDWE"/>
    <dbReference type="OrthoDB" id="1515497at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102208">
        <property type="term" value="F:2-polyprenyl-6-hydroxyphenol methylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008425">
        <property type="term" value="F:2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008689">
        <property type="term" value="F:3-demethylubiquinone-9 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00472">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010233">
        <property type="entry name" value="UbiG_MeTrfase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01983">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="239" mass="27186" checksum="5D5FE67DB132E0E2" modified="2005-09-27" version="1">MNQTVNQDELLKFSKIASQWWDENGPFKALHSMHPLRMQYIVDYINKVTPGVDFKKLSVLDVGCGGGLLSESMARLGMKVFGIDASEESVNIAKVHALNRNLCNLDYCCVNVDSLVSTKEKYDIITVMEVVEHVDNLSVFINSISNILNDNGIIFLSTLNRTMKSLLYAIVYAEYVFKLVPRHTHQWNKFVKPSEIANLFLLNDISIKEIKGINFNIMKKDWVLSDDISVNYILSAQKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS61</accession>
    <name>Q3YS61_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribose/galactose isomerase</fullName>
            <ecNumber evidence="5">5.3.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0401</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LacAB/RpiB family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304522.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0401"/>
    <dbReference type="EnsemblBacteria" id="AAZ68444">
        <property type="protein sequence ID" value="AAZ68444"/>
        <property type="gene ID" value="Ecaj_0401"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0401"/>
    <dbReference type="eggNOG" id="COG0698">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091396_4_1_5"/>
    <dbReference type="OMA" id="ETHHANQ"/>
    <dbReference type="OrthoDB" id="1346802at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004785">
        <property type="entry name" value="RpiB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003500">
        <property type="entry name" value="RpiB_LacA_LacB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036569">
        <property type="entry name" value="RpiB_LacA_LacB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30345">
        <property type="entry name" value="PTHR30345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02502">
        <property type="entry name" value="LacAB_rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005384">
        <property type="entry name" value="RpiB_LacA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89623">
        <property type="entry name" value="SSF89623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01120">
        <property type="entry name" value="rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00689">
        <property type="entry name" value="rpiB_lacA_lacB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="3">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="3">
        <location>
            <begin position="71"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005384-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005384-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="149" mass="16678" checksum="D78BE1A6E4297DD5" modified="2005-09-27" version="1">MLVMQKVFISSDHAGVELRLVIKLYLNNLGYIVEDYGCVESGVPVDYPDYVQNVVDCVINDSLAFGILICGTGIGMSIVANRHRKIRAALCYNVEVARLAREHNDANVLCLGARHISYEDAQSIVYNFLTTKFSEGRHVQRIIKIDNCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS87</accession>
    <name>Q3YS87_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional protein PutA</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="2">Proline dehydrogenase</fullName>
                <ecNumber evidence="2">1.5.5.2</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2">Proline oxidase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="2">Delta-1-pyrroline-5-carboxylate dehydrogenase</fullName>
                <shortName evidence="2">P5C dehydrogenase</shortName>
                <ecNumber evidence="2">1.2.1.88</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Oxidizes proline to glutamate for use as a carbon and nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + L-glutamate 5-semialdehyde + NAD(+) = 2 H(+) + L-glutamate + NADH</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a quinone + L-proline = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the proline dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="2"/>
    <dbReference type="EC" id="1.2.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0375"/>
    <dbReference type="EnsemblBacteria" id="AAZ68418">
        <property type="protein sequence ID" value="AAZ68418"/>
        <property type="gene ID" value="Ecaj_0375"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0375"/>
    <dbReference type="eggNOG" id="COG0506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005682_1_0_5"/>
    <dbReference type="OMA" id="PPWNFPV"/>
    <dbReference type="UniPathway" id="UPA00261">
        <property type="reaction ID" value="UER00373"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:1-pyrroline-5-carboxylate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006561">
        <property type="term" value="P:proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:proline catabolic process to glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025703">
        <property type="entry name" value="Bifunct_PutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005933">
        <property type="entry name" value="Delta1-pyrroline-5-COlate_DH-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024089">
        <property type="entry name" value="PRODH_PutA_dom_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024082">
        <property type="entry name" value="PRODH_PutA_dom_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14850">
        <property type="entry name" value="Pro_dh-DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000197">
        <property type="entry name" value="Bifunct_PutA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81935">
        <property type="entry name" value="SSF81935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01238">
        <property type="entry name" value="D1pyr5carbox3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 2 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Pro_dh-DNA_bdg" evidence="7">
        <location>
            <begin position="69"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Pro_dh" evidence="6">
        <location>
            <begin position="192"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="570"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="803"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000197-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1049" mass="116314" checksum="755930F13492B4B0" modified="2005-09-27" version="1">MGDIGMISALQAPNEIRKRMQMLYRTEENSYARYLTEKTEVSQDSKIRIYSVAKQIIEKIRVDRNLGIIDAFMQEYGLSNEEGIALMCLAESLLRIPDDCTINDLIKDKIGNSMWSNHIGSSSSMFVNAATWGLFIGGIVLKESNDSAKWFGTINNLLKTMGEPIIRKAIQQAMCTLGKHFIKGRDITEALNNRKEGELYSFDILGEAAKTRKDAERYFSEYMQAVDSIGKSKGSDDTGRLVDYDEISVKISALHSRYEFSQIDDVLDEIVDKLLQICRLAKEYNIRVCIDAEEASRLEISLMILEKLRFESSLNGWEGLGLAVQAYQKRAFSVLDFVEDISVRSCHKMMVRLVKGAYWDYEIKNSQELGLSSYPVFTRKVYTDVSYLACANKILSKPNTFYPCFATHNAYTLAAILEMANKDHPGFEFQRLHGMGASLYEYVTQELAANIKCRVYAPVGGYQDLLPYLIRRLLENGANSSFINQLNDSNISLEQLIQDPLEKAKELEYLPHPNIPLPKDIFGPERLNSSGIDITDSVTLANFNDEMKNYQNCKFKASSIVNGEEFDGDFIEILSPSNSEDLVGEVLFASSTQALSALDIAYSAFKDWSNVPVSTRASILEKAANLIEENKAKLIMLLIREGGKVISDAIAEIREAVDFLRYYAVLGRQELEGSNRLPGPVGEDNYLYFRSRGVFVCISPWNFPLAIFIGPIAAALVTGNTVIAKPAEQTSIIAYEAVKLLYDAGIPKGVLHLLLGDGKELGEVLLKNEKIGGVAFTGSTETARIINQSIAEKEGGIIPFIAETGGLNTMITDTSALVEQVTNDVITSAFKSAGQRCSALRVLFVQEEVADKQIEMICGAMEDLVIGDPMLLKTDIGPVIDKASQEMLIAHADRMSQEGKLLCQVKLGEECQKGYFFAPCAYEIQNISQLQREVFGPILHIIRYKKGDLHKILSEINDTGYGLTFAVQSRVQSNIDNIIDNINVGNVYVNRNQVGAVVGVQPFGGQGLSGTGPKAGGPYYLHRFLTEKVVSINTTALGGNTSLMCLTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS92</accession>
    <name>Q3YS92_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="7">Glutamate N-acetyltransferase</fullName>
                <ecNumber evidence="7">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="7">Ornithine acetyltransferase</fullName>
                <shortName evidence="7">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="7">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="7">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="7">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="7">N-acetylglutamate synthase</fullName>
                <shortName evidence="7">AGSase</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="7" type="primary">argJ</name>
        <name evidence="8" type="ordered locus">Ecaj_0370</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Heterotetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ArgJ family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="7"/>
    <dbReference type="EC" id="2.3.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304491.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0370"/>
    <dbReference type="MEROPS" id="T05.001"/>
    <dbReference type="EnsemblBacteria" id="AAZ68413">
        <property type="protein sequence ID" value="AAZ68413"/>
        <property type="gene ID" value="Ecaj_0370"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0370"/>
    <dbReference type="eggNOG" id="COG1364">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027172_1_0_5"/>
    <dbReference type="OMA" id="DYVHENS"/>
    <dbReference type="OrthoDB" id="1083409at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:acetyl-CoA:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103045">
        <property type="term" value="F:methione N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="PTHR23100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5023261576" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="7">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023261575" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="7">
        <location>
            <begin position="207"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="7">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="7">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="7">
        <location>
            <begin position="206"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="423" mass="46069" checksum="DA19DD2AD5152114" modified="2005-09-27" version="1">MGRKFSDHPRFLVSPFAPTDFPIMPDVNGVELYSFASGIKKIVDFSGKLDLLIVKMPCNTSVAGVFTTSYTCSSNVEHCRKILPHGKARVLVVNSGNSNVSVGVGGYKAVEEVTRLCSERFGCSTEEVYFCSTGVIGVPLPVDKITSVILNPDWSSAKNSWDAAADSITTTDTFIKMVTKEVSICGEKVTINGICKGSGMVAPNMATMLAYIFTDANIKSDVLQKLLREYVDISFNSITVDGDTSTSDSVLMFATNLAQHKQVCSADDQDIDDFKIALKDMLLELAHFIVKDGEGLTKFITINIRNAVSLSSARKIGFSVANSLLVKTAIAGGDANWGRIMMAIGKAGEPFNKNEVSLKIGDSVVLINGEISPTYDRNFVNEYIKNNQNIYIEVDINVGNFDATVWTCDLTHRFVDINGKYLT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS95</accession>
    <name>ASSY_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate synthase</fullName>
            <ecNumber evidence="1">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argG</name>
        <name type="ordered locus">Ecaj_0367</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304488.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS95"/>
    <dbReference type="SMR" id="Q3YS95"/>
    <dbReference type="STRING" id="269484.Ecaj_0367"/>
    <dbReference type="EnsemblBacteria" id="AAZ68410">
        <property type="protein sequence ID" value="AAZ68410"/>
        <property type="gene ID" value="Ecaj_0367"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0367"/>
    <dbReference type="eggNOG" id="COG0137">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032784_4_2_5"/>
    <dbReference type="OMA" id="QCEVVTF"/>
    <dbReference type="OrthoDB" id="357142at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="Argininosuccinate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="PTHR11587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="SSF69864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000263923" description="Argininosuccinate synthase">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <sequence length="393" mass="44353" checksum="4444B569FA3174C9" modified="2005-09-27" version="1">MKKIVLAYSGGLDTSVILKWLQENYNCEVVVFTADLGQDDDMSVIRQKATALNVKEIFIEDLKEEFVKDFVFPMFRANTVYEGYYLLGTSIARPLIAKRQIEIAKLTGADTVAHGATGKGNDQIRFEFGYYCCNPNIKVIAPWRQWKLTSRNSLIEYAKKHGINVPLDKSSEPPYSIDANLLHTSYEGKSLEDPYVEPDYTILSKSVTPELASDTPEYIEISFEKGDPCAINNIPLSPANLLKQLNTIGGKHGIGIIDIVENRYVGIKSRGIYETPGGTILLHAHRAIESITLDREAAHLKDEIMPKYAKLIYNGYWWTTERKMLQALIDRSQEKVNGVVRLKLYKGSVIVVGRKSENSLYSHNLASFDLSEDYNHTDAEGFIKINSLRLRNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS97</accession>
    <name>Q3YS97_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Gp200</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304486.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YS97"/>
    <dbReference type="STRING" id="269484.Ecaj_0365"/>
    <dbReference type="EnsemblBacteria" id="AAZ68408">
        <property type="protein sequence ID" value="AAZ68408"/>
        <property type="gene ID" value="Ecaj_0365"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0365"/>
    <dbReference type="eggNOG" id="COG0666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_250614_0_0_5"/>
    <dbReference type="OrthoDB" id="1360387at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="SSF48403"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="410"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="445"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="478"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="733"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="767"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="800"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="940"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="1103"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1397"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1421" mass="152933" checksum="43D7D8CF430DA2EE" modified="2005-09-27" version="1">MSDPKQGDPEQNQTNPSGDIQDQSQQDQQEQDQQQGAVGGAVGNSPIERERVAAPESEDLYTVIIPKGKRTAAPILERKSPTPEPKVEDDEDLPPTLPPRTFSGEGYDDVGVSMPTVSRGIYQPPIVQDSNLYSSIGGVPQEAQYDAAARAGGPRKFLYGPYTFSNGQEIMDFEFDTPWPDVRNAVLGNKEIKEEWLTTSGPVRDIADRIVASKGDLSEDQVEEILDIIFMNESEIAEGISNPLHADVDNNPVKGAKNVMTLMHLVYACDVDPRIVKALGEVENDEGDLGANAYNVLDSEGNLPLHHAAKNCTGDKLKLCMEKTKTDFIDTANFANQSPLHIITQKPDCSVLDIEEFTSRNLDFGLVDGDGKNPLHHAVEHLPPVILKGVMDHVKNSSEFQDLVNDPDYFGNTIAHYAVKNKNADLTLFNMLKASGADLNVRNVVGRAPIHVASSNGKANAVSGLVSCGIDVNSQDVNGDTPLHIAVEGGSMETVLAVLNQRGADVSVQNNDGVTPMLSAAKYGDIGVIKALGSAKPNIKGEDTVAKSLLMEDYKGFTPLHFVAGGGSRDTFRVVRKNYEKCHDLATIRAALMQDRSGGELVNLGDFESENILGSPNAKFLQHIQSANFGFSPAHCAIVSSNHNVMKDILNFVGDSLHLPSERGYNAMQVAALFGDKEAVKMLAKSAKPSDLNFKTSATPTPLNLACLRGDNEVVRGLVGQHGIDINQRMGSDKNTVLHYAISKGDSFLVQKILAHTGVDVNCENNLGQTPLHLAVEGGDPKIVSSLLKAGAVVNRLDDNGRSVLSSAIVPGRKEKGVLGIVNKLLDRGADINLDGDHNILFDQCLRGGYNNVLDKLIQQGVEVNRNSEIRPMVYAAISGNEHAIKSLANAGGDVNEVVNNPSSRHSGNPLIMVAVADGNAGLLKTLVSEGCDVGKSGKDGNTALHYAVSHSDKEFGNKAIKILISRNSVGTNRDILTQKNNAGDTPLHEALKSGNINSVQNILSAVHPRYAKEILTARDKEGYTPMHYTVGVNNVDVGRSILESMLSKGVNNLGEIVGAQDSNFRTPLHAAIKISDYRAADMIIGSLSKTELSKLSQLTDINGDTPLHLSCQSGNVEMTQFFLGGLDKRELPKTLKIANKNGDTPLHDAIRNDDIKSAKMMIRNCNKEELANVLKCKDSFGNTVLHTIADQVIANPESKKDLDGLMNLAVKRLKNQDLKDLVNTRNNSDDTVAHCALLSDMKYAQKILKSCNHDTLVRGNSNNQSLSECIRDDSKYKKGGIFSKSLFSKLKKLEARAASASYEELSSISSGSDVSSVSTNSTEVSAVPEVARSSGAVSFKHVQETGVDTSGPSDIESLERLSDTSLGSNDFDQRMADLDQEIANIVSGLPEVTQVAVSQQQAASPSSGQAAGVQQKEMQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSA3</accession>
    <name>Q3YSA3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal subunit assembly factor BipA</fullName>
            <ecNumber evidence="3">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">GTP-binding protein BipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bipA</name>
        <name evidence="5" type="ordered locus">Ecaj_0359</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="3">Binds to ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. BipA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304480.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0359"/>
    <dbReference type="EnsemblBacteria" id="AAZ68402">
        <property type="protein sequence ID" value="AAZ68402"/>
        <property type="gene ID" value="Ecaj_0359"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0359"/>
    <dbReference type="eggNOG" id="COG1217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017016_4_0_5"/>
    <dbReference type="OMA" id="MSMLFTI"/>
    <dbReference type="OrthoDB" id="143460at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03710">
        <property type="entry name" value="BipA_TypA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00849">
        <property type="entry name" value="BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035651">
        <property type="entry name" value="BipA_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042116">
        <property type="entry name" value="TypA/BipA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006298">
        <property type="entry name" value="TypA_GTP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF8">
        <property type="entry name" value="PTHR42908:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01394">
        <property type="entry name" value="TypA_BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="8"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="20"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="614" mass="67495" checksum="9911901DA1546B60" modified="2005-09-27" version="1">MLSQGDYQSICNLAIIAHVDHGKTTLLDSMLKQSGTFRENQDVAERVMDNNDLERERGITILAKCTSVIWQGKKINIIDTPGHADFGGEVERVLSMADGVLLLVDASEGPMPQTKFVLSKALKAGLLPIVIINKVDRPDSRIDEVLDEVYELFINLDATSEQLDFPILYASGRNGWCIKNLSDNRESLVPLFEAIVQYVKPVAYDANAPFSMLLTLLESDKFLGRILTGKIYTGTARVNDGVKVLSLSGEVVEYGKLTKLLAFSGLKRVPVKEAKAGDIIAVAGLEKASVSDSILDSSINFPIASTPVDPPTMAVTIGVNDSPFAGTEGVKLTSTVIKARLIAEAETNVAITVKETGQGDAYEVGGRGELQLGVLIETMRREGFELSVSRPRVLLRKDDKGQVLEPIEEVVIDVDEAYSGVIMEKLSFRKGDMRDLRPSGKDRVRMVFLVPSRGLIGYQGEFLTDSHGTGIMNRLFHSYAPHKGNIPTRNNGVLISTDKGDAVAYAIFNLQDRGVMFIKPQDKVYCGMIVGLHNRENDIDVNVLKGKQLTNIRAAGSDEAVRLVPPKIMTLEEMIAFINDDELVECTPKSVRLRKKYLDPNERKRASRANKNMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSC3</accession>
    <name>Q3YSC3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304460.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0339"/>
    <dbReference type="EnsemblBacteria" id="AAZ68382">
        <property type="protein sequence ID" value="AAZ68382"/>
        <property type="gene ID" value="Ecaj_0339"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0339"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046586_0_3_5"/>
    <dbReference type="OMA" id="VCHEMLA"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="66"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="263" mass="30254" checksum="85E3CC5BC5F2ECCC" modified="2005-09-27" version="1">MLVFDRSLVRLYRERLYNKDSNFVFSEISDLLLDKLSLFSLTAGLMLNIGIRSNSFSEDLLLRKIVSSHDQIIQCDLSYYVLCNARGNCKVVADEEALPFCSDIFDIVVSNASLHNVNNLFNILLSIYNIMKRRGIFLAALFGSKTLYELKHSMIRAEMDFGIAPRVLPFISVQDIVSLLQKTGYSDIVVDVNTIKVEYDDIYALFKDLRNMGEGNVLYNRNKYPLSRTVIRKIFESYKQYFSIDKVNIPVTFEIITLKCSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSE3</accession>
    <name>Q3YSE3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CutA1 divalent ion tolerance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304440.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0318"/>
    <dbReference type="EnsemblBacteria" id="AAZ68362">
        <property type="protein sequence ID" value="AAZ68362"/>
        <property type="gene ID" value="Ecaj_0318"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0318"/>
    <dbReference type="eggNOG" id="COG1324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098807_3_0_5"/>
    <dbReference type="OMA" id="VYTTFPD"/>
    <dbReference type="OrthoDB" id="1801957at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004323">
        <property type="entry name" value="Ion_tolerance_CutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419">
        <property type="entry name" value="PTHR23419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="106" mass="12114" checksum="847D27495C63F2AD" modified="2005-09-27" version="1">MTNISLIYTTTPTYEDAYRISNILLNNKLIACANIFNNITSVYLWENEIHNNSECAIILKTTNDLVHAATDKIQEIHPYDTPAIITIDPTNTNDKFTHWINDCITL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSG0</accession>
    <name>EFP_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor P</fullName>
            <shortName evidence="1">EF-P</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">efp</name>
        <name type="ordered locus">Ecaj_0301</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the elongation factor P family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304423.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSG0"/>
    <dbReference type="SMR" id="Q3YSG0"/>
    <dbReference type="STRING" id="269484.Ecaj_0301"/>
    <dbReference type="EnsemblBacteria" id="AAZ68345">
        <property type="protein sequence ID" value="AAZ68345"/>
        <property type="gene ID" value="Ecaj_0301"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0301"/>
    <dbReference type="eggNOG" id="COG0231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074944_1_1_5"/>
    <dbReference type="OMA" id="WSVVEFQ"/>
    <dbReference type="OrthoDB" id="1260763at2"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04470">
        <property type="entry name" value="S1_EF-P_repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05794">
        <property type="entry name" value="S1_EF-P_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00141">
        <property type="entry name" value="EF_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015365">
        <property type="entry name" value="Elong-fact-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020599">
        <property type="entry name" value="Transl_elong_fac_P/YeiP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013185">
        <property type="entry name" value="Transl_elong_KOW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001059">
        <property type="entry name" value="Transl_elong_P/YeiP_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011768">
        <property type="entry name" value="Transl_elongation_fac_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30053">
        <property type="entry name" value="PTHR30053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01132">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08207">
        <property type="entry name" value="EFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09285">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005901">
        <property type="entry name" value="EF-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01185">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00841">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00038">
        <property type="entry name" value="efp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000010737" description="Elongation factor P">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00141"/>
        </source>
    </evidence>
    <sequence length="189" mass="21323" checksum="19455CCD8CE40D50" modified="2005-09-27" version="1">MAERGSDIRPGHILEHNNALYLVVKVMHTQPGKGGAYIQAEMKNLKTGAKQYERFRTDGDIKRAIVDESDYQYIYGDGSMITVMHLKTYEQITISKDILGDKSIYLQDNIVITLVFYNGEIISAKVPDYVTLRVIETEAVIKGQTVSSSSYKVAMLENNQRISVPTFIKPGDKIVVYTPDDSYYERAKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSH2</accession>
    <name>CCME_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytochrome c-type biogenesis protein CcmE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytochrome c maturation protein E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heme chaperone CcmE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ccmE</name>
        <name evidence="1" type="synonym">cycJ</name>
        <name type="ordered locus">Ecaj_0286</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
            <orientation evidence="1">Periplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CcmE/CycJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304411.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSH2"/>
    <dbReference type="SMR" id="Q3YSH2"/>
    <dbReference type="STRING" id="269484.Ecaj_0286"/>
    <dbReference type="EnsemblBacteria" id="AAZ68333">
        <property type="protein sequence ID" value="AAZ68333"/>
        <property type="gene ID" value="Ecaj_0286"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0286"/>
    <dbReference type="eggNOG" id="COG2332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079503_1_1_5"/>
    <dbReference type="OMA" id="HVEFAVH"/>
    <dbReference type="OrthoDB" id="1724595at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017003">
        <property type="term" value="P:protein-heme linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01959">
        <property type="entry name" value="CcmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004329">
        <property type="entry name" value="CcmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036127">
        <property type="entry name" value="CcmE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34128">
        <property type="entry name" value="PTHR34128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03100">
        <property type="entry name" value="CcmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82093">
        <property type="entry name" value="SSF82093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0735">Signal-anchor</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000238806" description="Cytochrome c-type biogenesis protein CcmE">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Signal-anchor for type II membrane protein" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="topological domain" description="Periplasmic" evidence="1">
        <location>
            <begin position="29"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01959"/>
        </source>
    </evidence>
    <sequence length="133" mass="14989" checksum="AA7F63C85D04BE3A" modified="2005-09-27" version="1">MKRKHKRLLFIIVTFIIFGSSVVIVLNKLRSNISFFFTPTEVLSGSINVSSNDIRIGGMVISGSLKRYDNLISFFITDLESQIKVVYQGILPPLFSEGSWVVAKGKMVNGEFNANEILAKHDENYKPGKYRAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSH4</accession>
    <name>PYRF_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="1">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">OMP decarboxylase</fullName>
            <shortName evidence="1">OMPDCase</shortName>
            <shortName evidence="1">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrF</name>
        <name type="ordered locus">Ecaj_0284</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + orotidine 5'-phosphate = CO2 + UMP</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the OMP decarboxylase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304409.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSH4"/>
    <dbReference type="SMR" id="Q3YSH4"/>
    <dbReference type="STRING" id="269484.Ecaj_0284"/>
    <dbReference type="EnsemblBacteria" id="AAZ68331">
        <property type="protein sequence ID" value="AAZ68331"/>
        <property type="gene ID" value="Ecaj_0284"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0284"/>
    <dbReference type="eggNOG" id="COG0284">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067069_1_0_5"/>
    <dbReference type="OMA" id="NFKIFLD"/>
    <dbReference type="OrthoDB" id="1150446at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01200_B">
        <property type="entry name" value="OMPdecase_type1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="PTHR32119:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241856" description="Orotidine 5'-phosphate decarboxylase">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01200"/>
        </source>
    </evidence>
    <sequence length="228" mass="24871" checksum="9C11E5E990C9ECB1" modified="2005-09-27" version="1">MWCNPIICALDTYDINHALRLTKMLYGKISMVKLGLEFFTAHGLSGVQAVSDCGIPIFLDLKLHDIPNTVGKAISVIKSLNIAMLTIHISGGRDMMLSAIDSIFGSMIKLIGVTVLTSIDDSDLKDIGIDRSSIQHVMLLSKVAQEIGLYGIVCSAFEVQEVRNQCGKDFKLVVPGIRFEDDYSDQKRVKNPKDAILAGADYLVIGRPITMSSDPIQTVDAILSSINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSH7</accession>
    <name>Q3YSH7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH_37 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304406.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0281"/>
    <dbReference type="EnsemblBacteria" id="AAZ68328">
        <property type="protein sequence ID" value="AAZ68328"/>
        <property type="gene ID" value="Ecaj_0281"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0281"/>
    <dbReference type="eggNOG" id="COG5606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163934_1_0_5"/>
    <dbReference type="OMA" id="NHWNQVT"/>
    <dbReference type="OrthoDB" id="1946648at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039554">
        <property type="entry name" value="HigA2-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13744">
        <property type="entry name" value="HTH_37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_37" evidence="1">
        <location>
            <begin position="5"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="108" mass="12178" checksum="64E351ADAB352AB2" modified="2005-09-27" version="1">MTTISNQNDYGIKYKILKLIKDITYKNHWNQVTTAKILGVDQPKISQISNGKTAGFSLERLLIFLLRLKCEVNITITINNSEIALINKDSITENSLESINLVIVQKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSI6</accession>
    <name>Q3YSI6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nicotinate-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Deamido-NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Deamido-NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Nicotinate mononucleotide adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0272</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H(+) + nicotinate beta-D-ribonucleotide = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NadD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.18" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304397.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0272"/>
    <dbReference type="EnsemblBacteria" id="AAZ68319">
        <property type="protein sequence ID" value="AAZ68319"/>
        <property type="gene ID" value="Ecaj_0272"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0272"/>
    <dbReference type="eggNOG" id="COG1057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069765_2_0_5"/>
    <dbReference type="OMA" id="RWFERYE"/>
    <dbReference type="OrthoDB" id="1433958at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="11">
        <location>
            <begin position="16"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="194" mass="22969" checksum="25B6411A62ACEAE3" modified="2005-09-27" version="1">MFFNKFIVVRQLKIGLLGGSFNPPHYGHLYITQEAIKRLDLDCVWWLVVSRNPLKFNGGYSIEDRVTLSLQLVASYPKVRVIKVTECYSYNVVTRLCKKFVNVKFVWLMGDDNLFSFHYWYRWKAFCKLLPIVVFERSKNIYRCLGTPFVSYMRNAYCVDFHLLLNCRYGWILVRLMPCNVSSSQIRGYTLNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSI8</accession>
    <name>Q3YSI8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10 11">Chromosomal replication initiator protein DnaA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dnaA</name>
        <name evidence="15" type="ordered locus">Ecaj_0270</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10 11">Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 12">Belongs to the DnaA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0270"/>
    <dbReference type="EnsemblBacteria" id="AAZ68317">
        <property type="protein sequence ID" value="AAZ68317"/>
        <property type="gene ID" value="Ecaj_0270"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0270"/>
    <dbReference type="eggNOG" id="COG0593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026910_3_0_5"/>
    <dbReference type="OMA" id="REFNPLF"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06571">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1750.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00377">
        <property type="entry name" value="DnaA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001957">
        <property type="entry name" value="Chromosome_initiator_DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020591">
        <property type="entry name" value="Chromosome_initiator_DnaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018312">
        <property type="entry name" value="Chromosome_initiator_DnaA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013159">
        <property type="entry name" value="DnaA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024633">
        <property type="entry name" value="DnaA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038454">
        <property type="entry name" value="DnaA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30050:SF2">
        <property type="entry name" value="PTHR30050:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08299">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11638">
        <property type="entry name" value="DnaA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00051">
        <property type="entry name" value="DNAA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00760">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00362">
        <property type="entry name" value="DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01008">
        <property type="entry name" value="DNAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="13">
        <location>
            <begin position="150"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_DnaA_C" evidence="14">
        <location>
            <begin position="361"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="158"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAZ68317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="452" mass="51542" checksum="011BBBB28C80B47B" modified="2005-09-27" version="1">MKEGLQSDNRVIWKDIQSKLHKFYGNAIYDSWLSVLVYVNNEGGKVLLSAPTRFIKEWILVHYLDQILKYWQDEDKSVCSVDICVVNNQDTNLIVNVKDQQGNEIRNNYDHLSSPLDPRFTFDNFVVGKPNELAFAAARRVAESNDPISGSNPLFLYGGVGLGKTHLMHAIAWYIIKSSSKRKIAYLSAEKFMYQYVTALRSKDIMLFKEQFRSVDILMVDDVQFISGKDSTQEEFFHTFNALIDQNKQLVISADRSPSDLDGVEERIKSRLGWGLVADINETTFELRLGILQLKVEKMGINIPNKVLEFLAKNIKSNIRELEGALNKVVAHSSLVGCSITLDSASDILSDLLRANHKSVTLECIQKKVAEFFNIKMTDMYSTRRLRTLARPRQVAMYLSKKLTQKSLPEIGKSFGGRDHATVIHAVKQIEKLIETDDKLRDDINLLTRMLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSJ3</accession>
    <name>Q3YSJ3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0265</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304390.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0265"/>
    <dbReference type="EnsemblBacteria" id="AAZ68312">
        <property type="protein sequence ID" value="AAZ68312"/>
        <property type="gene ID" value="Ecaj_0265"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0265"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_0_5"/>
    <dbReference type="OMA" id="FQYVSMT"/>
    <dbReference type="OrthoDB" id="961781at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="9"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="411" mass="46626" checksum="72B5D12F169657F3" modified="2005-09-27" version="1">MSTRSSVKPILSILICAFVECYDFLIYGNFSRMFSKMFFAHITEDFAMVLSLMSFGMAFFVRPFGSLLFGYIGDKYGRKTALFSSATLLIISVGGIAFLPLFETIGVLSPILLIVFRVLQGLSFAGEIGAVVLMVENAKNTRNIIYAMYSHFIVGIFGGAVGCYIFKLCNNLIPEYQFYSWGWRIPFIVGLLMSFSLPFLRNSIEESREYLYYNNKNKISKVPIVDIILNYKYPFIMVCIFVPFCNALFYTFFVFLEVQSKVNVLIYSLLILTIVLSGYLISLICRVYKTTTVVFSANIFFIVIISPLIWFFGESIFTYFLTALSLGMASTPLSAILMLLFPANVRQTGYSVAFSIGIGCFGMLTPMIFLWLSQLGLSTVFCMDLYALFSLYGFYCLKKDKNVIKYESTFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSK1</accession>
    <name>DAPA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="1">HTPA synthase</shortName>
            <ecNumber evidence="1">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapA</name>
        <name type="ordered locus">Ecaj_0257</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304382.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSK1"/>
    <dbReference type="SMR" id="Q3YSK1"/>
    <dbReference type="STRING" id="269484.Ecaj_0257"/>
    <dbReference type="EnsemblBacteria" id="AAZ68304">
        <property type="protein sequence ID" value="AAZ68304"/>
        <property type="gene ID" value="Ecaj_0257"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0257"/>
    <dbReference type="eggNOG" id="COG0329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_7_1_5"/>
    <dbReference type="OMA" id="GMDACVP"/>
    <dbReference type="OrthoDB" id="1438588at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_1000050187" description="4-hydroxy-tetrahydrodipicolinate synthase">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="1">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="296" mass="32630" checksum="3827263918354B28" modified="2005-09-27" version="1">MSKVKLSGIFTALITPFKNDFSIDEDTFSKLIEHQISNNIHGLVPCGTTGEYSTLSFEEYCRVIELCVKVTNKRVPIIAGSGSNCTQETIKRTLYVQSLNVDAALVVVPYYNRPSDEGIFQHFKAVHDATDVPIIIYNIPQRTAIDPNDVLLARILSLPRIIGIKDATGDVSRPLNLKLLIDKEFALFTGNDATSLGFYAQGGGTGCISAVSNVIPKIYSDMHNAFFAHNIKEAMDANASIFKLSKVLFCQSNPSPTKYAMSLIKGISPTVRLPLVELTQENKLKVENMLKELNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSL8</accession>
    <name>Q3YSL8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Adenylosuccinate lyase</fullName>
            <shortName evidence="10">ASL</shortName>
            <ecNumber evidence="4 10">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0238</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = AMP + fumarate</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="4 10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304365.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0238"/>
    <dbReference type="EnsemblBacteria" id="AAZ68287">
        <property type="protein sequence ID" value="AAZ68287"/>
        <property type="gene ID" value="Ecaj_0238"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0238"/>
    <dbReference type="eggNOG" id="COG0015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_0_1_5"/>
    <dbReference type="OMA" id="ASSCEKI"/>
    <dbReference type="OrthoDB" id="347727at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADSL_C" evidence="11">
        <location>
            <begin position="350"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="433" mass="49870" checksum="1222DD083ABB86EB" modified="2005-09-27" version="1">MIKRYSRPKMSSIWEDNNKFKIWLDIEYYACEAQVKLGIIPSYILDNLKNKTEHFDINRINEIESVVKHDVIAFLTYIAESTNSDIRYLHYGMTSSDVLDTCLSVQLKQSCELLLHDIEIILSILKQKSIETKYMLCMGRSHGIHAEPITFGLKLARFYAEFKRNYNRLKIAQDEISVCKISGAMGNFANIDPFVENYVAKALNLKSESISSQVIPRDRHAMFFATLGVIASSIENIAIEIRHLQKTETLEVTEYFSSGQKGSSAMPHKKNPILSENLTGLARMIRSYVAPALENVALWHERDISHSSVERCIAPDACIVADFALARLADLLNNMIIYPNNMDKNLKQSKGLIFSQRVLLELVKHGLNRETAYKIIQERALETWNNNSHFLDELKRDPQVLKYINSKNLEDLFNLNYYTQHIDMIFDKVFQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSM0</accession>
    <name>Q3YSM0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304363.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0236"/>
    <dbReference type="EnsemblBacteria" id="AAZ68285">
        <property type="protein sequence ID" value="AAZ68285"/>
        <property type="gene ID" value="Ecaj_0236"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0236"/>
    <dbReference type="eggNOG" id="COG4649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1364414_0_0_5"/>
    <dbReference type="OMA" id="NIDNTKQ"/>
    <dbReference type="OrthoDB" id="1497321at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="200" mass="22562" checksum="AC43D28DFEF30BDB" modified="2005-09-27" version="1">MRLSLVKTLSKLKWVCVVAVMSLIVFAGMVSWVNSRNEKVSQHMGDMMYDALMNEISDDEMIKILNDVAKIDESSYQYLAKFKLASMYGEDKTEEAQSIYADLAADKRLTPELRELAEYLEVITLLKGNNLDLLKNKVQQFLSKRSEVYTSSIKEAIAVSMLKNNDISGAVGVIKEIVVSADSEPIVYKNAMDLLQIYEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSU4</accession>
    <name>RS7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsG</name>
        <name type="ordered locus">Ecaj_0160</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S9 and S11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304289.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSU4"/>
    <dbReference type="SMR" id="Q3YSU4"/>
    <dbReference type="STRING" id="269484.Ecaj_0160"/>
    <dbReference type="EnsemblBacteria" id="AAZ68211">
        <property type="protein sequence ID" value="AAZ68211"/>
        <property type="gene ID" value="Ecaj_0160"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0160"/>
    <dbReference type="eggNOG" id="COG0049">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072226_1_1_5"/>
    <dbReference type="OMA" id="NVMPHVE"/>
    <dbReference type="OrthoDB" id="1540940at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_B">
        <property type="entry name" value="Ribosomal_S7_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_S5/S7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005717">
        <property type="entry name" value="Ribosomal_S7_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_S7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_S7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="PTHR11205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="SSF47973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01029">
        <property type="entry name" value="rpsG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000226499" description="30S ribosomal protein S7">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <sequence length="160" mass="18307" checksum="31D17B0D5154D7A7" modified="2005-09-27" version="1">MSRRRRASKRVISPDSKYNSVLLARFINVIMRSGERSIAEKIVYGALNKAEARLGEGAMSIFNAALNNVMPQMEVRSRRIGGVTYQVPVEVKEDRAVSLALRWIFKAAAAARKRSNKMYMDCLCNELLEAYNKRGGAYKMREEKYKMAEANKAFSHFRFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSV0</accession>
    <name>Q3YSV0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0154</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0154"/>
    <dbReference type="EnsemblBacteria" id="AAZ68205">
        <property type="protein sequence ID" value="AAZ68205"/>
        <property type="gene ID" value="Ecaj_0154"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0154"/>
    <dbReference type="eggNOG" id="COG1178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021838_0_2_5"/>
    <dbReference type="OMA" id="QAFWRVA"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="460"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="505"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="48"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="327"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="539" mass="60864" checksum="42EE941805BA3E3B" modified="2005-09-27" version="1">MSFVNLSKNIFLCILIGMFIAPILSLISIIFTEHKTINLLTSVLPNYTFNTVVLMLGVGTIALVIGISAAWFVTYYSFFGRRFFEIALFLPLSIPGYIVAYVYVNIFEFSGPLQIFLRETFHWSKGDYYFPTVKSLEWGIIIIGFNLYPYVYMLVRTGLIAIRGTVAVATTLCPSRCKILTAIALPVVRPAIAASVSFVLMEVISDFGTPQFLAIDTFTSGIYRSWFLLHDKYSACLFAFIALFFIFLLIIFEKLFRGKGISYSTIKMNTEYYHTWQVNSKLKLTLIYCVCSIPILIGFVIPVVPLLYWTVERIGTLVTNTRFYMSVFNSISIAFITSVIAVIISIVMSYIIRKRESLSYAIRFIVMGYAIPNTIVAVSVMVLLGNISHFVNSYFSFALIGTTFGLIYAYVFRFLAVSLGPIESGLNKIPREIDWSSLLMGHNVISTCFNVHIPMIRKSILVGFLLVFVDVIKELAATLIIRPFNFDTMATLMYELISDERYADAAPYALVIVLIGLISVVILCKLFQYDKPECSKVML</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSV8</accession>
    <name>SYA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="1">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">alaS</name>
        <name type="ordered locus">Ecaj_0146</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAZ68197" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68197.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_044262106.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSV8"/>
    <dbReference type="SMR" id="Q3YSV8"/>
    <dbReference type="STRING" id="269484.Ecaj_0146"/>
    <dbReference type="EnsemblBacteria" id="AAZ68197">
        <property type="protein sequence ID" value="AAZ68197"/>
        <property type="gene ID" value="Ecaj_0146"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0146"/>
    <dbReference type="eggNOG" id="COG0013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_1_1_5"/>
    <dbReference type="OrthoDB" id="91428at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000075108" description="Alanine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="581"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="585"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="683"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="687"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="887" mass="99906" checksum="28B4A4DFD83DFAF5" modified="2005-12-06" version="2">MKESNLVGDIRKLFVDFFIKNGHQLFPSSPLIVKDDPSLLFTNAGMVQFKHVFTDASNANVGTAVSSQKCLRVGGKHNDLENVGYTNRHHTFFEMLGNFSFGDYFKEFAVELAWSFVTKELALNKDKLYFTVYHEDQETFDLWKKISGFSENRIIKIKTNDNFWSMGSTGPCGPCSEIFYDYGEDIEGGLPGTPEEDGARFTEIWNLVFMQYNRKSDGELCALPKKCIDTGMGLERISAVMQGVHDNYDINLFKDLIKVSKKQSGNTNNELAHRVIADHVRSAAFLIAEGLTPGNEGRDYILRRIIRRAARYVYMLKYDGALMYQIFPSLIDEKSYAYMADYYPELINAKDLIISILKIEEENFKDTLVKALPLLEKELVGLSSGGVLPGDIAFKLYDTYGFPVDITLDIIKEKGIKFDEQGFYDQMDKQKERSKLNHSIKSVQQLKGKLWVDIKEHYGGTKFVGYEQYSTRAKVLSIIYEGDKSTEVANVGDRVNVLLDITPFYAESGGQKGDTGVFNVIVRQGKELLSCDDVVEVLDTKKVLDTLYIHECVIKTGSLIVGDIICAEVNCEKRKNLCANHSATHLLHYVLKSVIDRSIIQKGSLVSDDKLRFDFSYGVALTKEQLTLIEDKMFSLIRNNSPVVTHICDLKEAIADGAVALFTEKYEDHGVRVINIGDSKELCCGTHVRYTGEIGCFKIVSEASVACGVRRIEAVTGQHAIDYFREQEKMLHLIAESVKSPIDNILIQIDKLNRNNQELKQKLSDAYFSVINLQGIATDKIGSIDFLYSSLNSVPIDVVRKFINEHLVNDMIMFFSNVVGRNVMYVIGVASNLHSKIKATDFVEIIGEVIKSKGGGNNQLAQISGEYIKEVDVIFHVKNKLINVLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSW0</accession>
    <name>Q3YSW0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutamine synthetase, catalytic domain</fullName>
            <ecNumber evidence="5">6.3.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304273.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0144"/>
    <dbReference type="EnsemblBacteria" id="AAZ68195">
        <property type="protein sequence ID" value="AAZ68195"/>
        <property type="gene ID" value="Ecaj_0144"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0144"/>
    <dbReference type="eggNOG" id="COG0174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1037204_0_0_5"/>
    <dbReference type="OMA" id="CRIEHRV"/>
    <dbReference type="OrthoDB" id="1372151at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="4">
        <location>
            <begin position="6"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="266" mass="30309" checksum="956993DF97BC8C7F" modified="2005-09-27" version="1">MLNYTLNYLNTNLNAIPLIGIELEFYFDNVEPNDISLLITNIKKKIFHFNCNIIKEQDNLQYEIQTSTTTNILNLILEINLIKEVLEDSINHFGGSINFAAKPYLDKPGSAFHIHINLLDANHNNLFYNQNNKMSNYLLYSIGGLCSLMKKHMIFFAPNNNSYLRYIHADIDTPTTISWGGNNRSTSIRIPSTSTDPTKCRIEHRVPGADCNYKQAITSILQGIIYGIENKIQPPPKTYGISSDIQYNLEKLPLSLNEAIKYNITD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSX3</accession>
    <name>Y131_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0434 protein Ecaj_0131</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Ecaj_0131</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0434 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSX3"/>
    <dbReference type="SMR" id="Q3YSX3"/>
    <dbReference type="EnsemblBacteria" id="AAZ68182">
        <property type="protein sequence ID" value="AAZ68182"/>
        <property type="gene ID" value="Ecaj_0131"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0131"/>
    <dbReference type="eggNOG" id="COG2835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155659_3_1_5"/>
    <dbReference type="OMA" id="ELICHAD"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01187">
        <property type="entry name" value="UPF0434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000291085" description="UPF0434 protein Ecaj_0131">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01187"/>
        </source>
    </evidence>
    <sequence length="56" mass="6503" checksum="794EEE2FA3246AA6" modified="2005-09-27" version="1">MIDHRILEILVCPLTKDKLQYNKDTNELISQKAKLAFPIRDGIPIMLIDEARKLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSX7</accession>
    <name>Q3YSX7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Surface protein-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304256.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68178">
        <property type="protein sequence ID" value="AAZ68178"/>
        <property type="gene ID" value="Ecaj_0127"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0127"/>
    <dbReference type="HOGENOM" id="CLU_005919_0_0_5"/>
    <dbReference type="OMA" id="AHLHENE"/>
    <dbReference type="OrthoDB" id="466263at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002169">
        <property type="entry name" value="Peptidase_M9A/M9B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01752">
        <property type="entry name" value="Peptidase_M9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1005" mass="113776" checksum="167F2DCB5C39C41F" modified="2005-09-27" version="1">MTIFLESDDDKSNFKKTLENGTKDKTNLDNTYYDYHHEDDMGNTEYHYVSLDRVDHVKMPEEPVGYGGDTLPIVPTTAASVSGSDAGVAVGNVKDFEDNVFHHTSTIRNDELKIDLRIHTLKDLSDKRLREIEKGFNDTVTKFKNNFGLEPNDGETIFDLYLFDDKEQYNYYGKLYNLGISGSGGMTFYGNANVPYKIYVHQYGEILNLKHELTHALESYASGHKLHGSDVNSRIFTEGLADYIQEDNSFIMRGLKDREITSDVLKDSSGNVDHLSGVAVNENQRLSYSIGHAFVSFLQEKYPKLISEYLNALKEDNIIRAKEIISMDKYPDFEPWVKSKDISLYLENMNVLKLGLGEKMFSAESASYFEDQGVNKEYYHENIYDMSGKLVGEMSPVVHYAQKNVIRIWNIASPDMIEVRPEYNFLKLVTTPSGKSAYVYCDKNGHEYFNTKDYIDSAFNILARYDVKLRESSDALDIRGRYSDAAKVFSKLPNADLLLDKFLEKIGYSSYKQIIMSNPEQLNSIKAYVVKEVFENFRESEVKKVLSGESHPEVRNVLMDLTYVDLKSVIGVNGADIDSIISNPDVMLRTAVLGKGNASGISLYVDDQKVGELSTEAGYCVKNLDTGKVYFMFHNVVGMIASGYEDRAYMVVLEKDGKFTTALVNNIQKAADGNVVWDNQFNHPNINNLHSNYKELLLNDASVKDYSHLADVKFNKDDTVIVKGELLDDKGTVSVDDDVHRAVVKHDDQILHQFKSMSFYITEPSADSGDNYGSDFFISDEGKNLRFQLPKAITHLKLVNVNGNNKLVPCTKDGNEHPEGMPSDLTDEYRYIDPIFAHTFEKQSYSKNSISVGLVDFSKYKEGSMFKLQHYSDDYHIHKDEQGNVIRPNNRSYVTKVDLVYDDKVIGMLSDSINQFQGDIFISASLNYSHNDFLSSKYFQKVNIEALENGIYSGRYDVGDGDQIAGLNTDTGYSDKAIFYFKNDSASTDMPASDVTTILPYINEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSX8</accession>
    <name>Q3YSX8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304255.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68177">
        <property type="protein sequence ID" value="AAZ68177"/>
        <property type="gene ID" value="Ecaj_0126"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0126"/>
    <dbReference type="HOGENOM" id="CLU_409240_0_0_5"/>
    <dbReference type="OMA" id="SKNYFFV"/>
    <dbReference type="OrthoDB" id="530639at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="671" mass="78143" checksum="339D6F9AEC678F06" modified="2005-09-27" version="1">MTSNTSIPKNHEYSFKLDIGENLYFFCNHNVHKVKIITEDNTEITMPSKNYFFVGDKFYAPYNNYFYDNYLNIPAEYRYIKVDHMQYRTTNNEQPQDFYNLVLCDKNGEEYRYNYYKFYIKPENIIEKSAEINLKEYYNIQQLKEGAPLFKIVSEQPNNTTKASTALILDISSNQKFAKLSPEALQYKHYLDRNSPTYDTFTLSYSDIRKHHVDEQEKINLHNIRDDILQAEMENNPIFLVIQDGKYFFTDVKQDQPLTTSYNTALKVLASANFQINNVPNDNCYVDMHKKFIFKITKSNLHTEHDNSKNLASITLEGKEIPLISNDDDTQIFYDDFSFKCYQNFTQVFNYDEPIIGLDKDFYEPIKEKLSSNNIYITIKSDEQNHIKTYFSDKQGNHILDLPNTKLTEYLSTMLPLGDFSNEVLNTHIEDIAHQKLSDTTQKHDTLNPEKNSTTLQNSVNETAGTNDPQSTQNAVHKHDTLDTQKDSTTSQKSVNDTASTNDSQSTQNAVHKHDTLDTQKDSTTSQKSVNETASTNDPQPTQNAVHKHDTLDTQKDSTTLQKLVSEEHNINKSNTNINVEQNIVYFPLSREHVSIVDNIEQNKHHVSFNLTYEEMLNFYEAVKEQYSYDEVLIAYNNIFKNYGREQKNDNIYIDGDNHIFIENHDFGILQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSZ0</accession>
    <name>PYRG_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTP synthase</fullName>
            <ecNumber evidence="1">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="1">CTP synthetase</shortName>
            <shortName evidence="1">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrG</name>
        <name type="ordered locus">Ecaj_0114</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304243.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSZ0"/>
    <dbReference type="SMR" id="Q3YSZ0"/>
    <dbReference type="STRING" id="269484.Ecaj_0114"/>
    <dbReference type="MEROPS" id="C26.964"/>
    <dbReference type="EnsemblBacteria" id="AAZ68165">
        <property type="protein sequence ID" value="AAZ68165"/>
        <property type="gene ID" value="Ecaj_0114"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0114"/>
    <dbReference type="eggNOG" id="COG0504">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_5"/>
    <dbReference type="OMA" id="EFNNAYR"/>
    <dbReference type="OrthoDB" id="783657at2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="PTHR11550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000266111" description="CTP synthase">
        <location>
            <begin position="1"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="295"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="1">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="510"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="512"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="19"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="151"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="191"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="191"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="384"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <sequence length="538" mass="60221" checksum="71E12065EA4A1413" modified="2005-09-27" version="1">MSNQTSSRFIFVTGGVVSSLGKGLAAASIGALLQARGFKICLRKLDPYLNIDPGTMSPIQHGEVFVTDDGAETDLDLGHYERFTGVKTTKNDNITTGKVYHNLLKKERRGDYLGQTVQIIPHVTDLINSFILHNTEGLDFVICEIGGTVGDIESQPFLESIRQIGYKLSKNNTIFVHLTLVPYIDAAMELKTKPTQHSVKELSAVGIQPDIILYRSKIPLSKEQRDKIANLCNVSPTNIIPALDVKNIYELPISYHNYNLDTQILKHFNVNSPDPNLSKWENILNISHTLTKTVTVAIIGKYIKLLDAYKSLIEALEHASIHNRVKLSIRWIDSRSLDSNGINAFDNVDSILIPGGFGDDGVKGKITAITHARLNKIPFLGICMGMQLAIVEFANNVAHLEDANSTEFNLYCKNPVIHQLPELQQNFLGGSMKLGSCPCYLDANSKIFSIYQQSVINERRRHRYTFNLQYKDLLEQHGLTFTGKANNTDDSLIEVIELNNHPWFIGVQFHPEFKSSPFHSHPLFISFIKASLSYQEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSZ8</accession>
    <name>Q3YSZ8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0106</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be required for disulfide bond formation in some proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thioredoxin family. DsbA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0106"/>
    <dbReference type="EnsemblBacteria" id="AAZ68157">
        <property type="protein sequence ID" value="AAZ68157"/>
        <property type="gene ID" value="Ecaj_0106"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0106"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1479845_0_0_5"/>
    <dbReference type="OMA" id="IYTSWCS"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13905">
        <property type="entry name" value="Thioredoxin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="148" mass="16844" checksum="1C2C3F317C04FF79" modified="2005-09-27" version="1">MPDTLIKIKEINSAQALSDMFKMTDSKINVIVIYTSWCSSCVKKIPEINKLISDFDNINPIVISLDENKNKLLSFLSKQGTLNFTPYNVSPSYLKNLLSYLTNKGIVFNHHIPYIAVLYNGIHPITNINNAKQLKLTIQNLIEKHHVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT09</accession>
    <name>Q3YT09_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Kef-type potassium/proton antiporter, CPA2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304224.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0095"/>
    <dbReference type="EnsemblBacteria" id="AAZ68146">
        <property type="protein sequence ID" value="AAZ68146"/>
        <property type="gene ID" value="Ecaj_0095"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0095"/>
    <dbReference type="eggNOG" id="COG0475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005126_9_3_5"/>
    <dbReference type="OMA" id="IRPFHDV"/>
    <dbReference type="OrthoDB" id="941006at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="6">
        <location>
            <begin position="408"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="569" mass="62424" checksum="7638D735F0FA4DB9" modified="2005-09-27" version="1">MHDSKYLLDIITLLSAAIVIVVLFWKLNISPVLGYFVAGTLIGSNGLNLVESSVIIQNLGEFGVVFLLFLIGIELTFERLIAMRLHVFGFGTLQVIVTASVIWYISNKFGIDSRGSLIIGGALALSSTAIVLQVLQEKGYQSTQVGRLSIAVLLLQDFAVVPLIVLLPLLTGESHHSIPISLLYSFIKAAIALTLIFITGRLLLRPLFNTIAAMKSNEIFIATTLLIVLGAAFITENFNLSMALGAFVSGLLVAETEYRHDVEQVVLPFKRLLLGLFFMTVGMSIDIKFVLNKLPIITLASISLIALKSFIIFILCRFFRFQLAAAIQAGLLLSQGGEFAFILFGLAAEKNVLSSDLAQTLMMITTVTMAFTPLLAALGDWISKLITKKKISLNSQELTLDTKDLDQHVIVVGFGRVGRMVAKVLVAEHVNYIASDIQPKIVKDGIDEGYPVYLGDLTKLNTLQSMAISRARMIIIAINNEVTIKKIISLVAKNFPHIIIIVRLPDLNNVEIYKELGAHYLIPETYEVGLQLGAVALSNSGFSNHAVALLKDRFRMVNYDVVKNKESDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT21</accession>
    <name>ATPA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit alpha</fullName>
            <ecNumber evidence="1">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpA</name>
        <name type="ordered locus">Ecaj_0083</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304212.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT21"/>
    <dbReference type="SMR" id="Q3YT21"/>
    <dbReference type="STRING" id="269484.Ecaj_0083"/>
    <dbReference type="EnsemblBacteria" id="AAZ68134">
        <property type="protein sequence ID" value="AAZ68134"/>
        <property type="gene ID" value="Ecaj_0083"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0083"/>
    <dbReference type="eggNOG" id="COG0056">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010091_2_1_5"/>
    <dbReference type="OMA" id="LQAPGVM"/>
    <dbReference type="OrthoDB" id="837522at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01132">
        <property type="entry name" value="F1_ATPase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01346">
        <property type="entry name" value="ATP_synth_alpha_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038376">
        <property type="entry name" value="ATP_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033732">
        <property type="entry name" value="ATP_synth_F1_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039088">
        <property type="entry name" value="F_ATPase_subunit_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00962">
        <property type="entry name" value="atpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000238244" description="ATP synthase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="171"/>
            <end position="178"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Required for activity" evidence="1">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01346"/>
        </source>
    </evidence>
    <sequence length="509" mass="55222" checksum="DF29819223AF6984" modified="2005-09-27" version="1">MNNTISPGEVLRVIKERIENFDNQVKADSVGEVISIKDGIAIVYGLEKAKFGEVVVFTNGVTGIVLGLDCDTASVVIFGNERSVGEGDTAKCTGKLMDVPVGFELLGRVVDALGNPIDGAGNVDSKIRLPVEIKAPGIIARQSVSEPLQTGIKVIDMLIPIGRGQRELIIGDRKTGKTAIAIDTIINQKLHNDTVSEKEKVYCIYVAIGQKNSAIARIVDKLRVSGALEYTIVVAAGASDTVSFQYLAPYAACAMGEFFRDNGMHCLIVYDDLSKHAVAYRQMSLLLRRPPGREAYPGDVFFLHSRLLERAAKMSDEKGAGSLTALPIIETQAGDVSAYVPTNVISITDGQIFLESEIFYKGFRPAVNVGLSVSRVGSAAQIKSVKKVAGSVKLSLAQYRELEDFAKFGSDIDVHSKKVLDRGRRMMELLKQKQYSPLSVAEQVAVIFAGTSGCLDDISVNNVGKFEEMLLKELNENYPDVLSNISNNFTDDVKDLLLDIISKVTSSFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT30</accession>
    <name>GUAA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="1">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">guaA</name>
        <name type="ordered locus">Ecaj_0074</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304203.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT30"/>
    <dbReference type="SMR" id="Q3YT30"/>
    <dbReference type="STRING" id="269484.Ecaj_0074"/>
    <dbReference type="EnsemblBacteria" id="AAZ68125">
        <property type="protein sequence ID" value="AAZ68125"/>
        <property type="gene ID" value="Ecaj_0074"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0074"/>
    <dbReference type="eggNOG" id="COG0519">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_5_5"/>
    <dbReference type="OMA" id="KRKIIGH"/>
    <dbReference type="OrthoDB" id="504464at2"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000229425" description="GMP synthase [glutamine-hydrolyzing]">
        <location>
            <begin position="1"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="GMPS ATP-PPase" evidence="1">
        <location>
            <begin position="200"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="227"/>
            <end position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00344"/>
        </source>
    </evidence>
    <sequence length="526" mass="58533" checksum="67C4089927AE486E" modified="2005-09-27" version="1">MSKVAIIDFGSQFTQLLARRIRELNVYCEIFTPDVEFDLVKDSKAFILSGGPKSVASLAEIPKIVHNIIELNKKSSIPVLGICYGLQLLSNYFNSAIVSDCSKEFGKATLNIIKESEITKNVWEVGKRVDVWMSHADSVYNVPRGFEVIAYSVLKNVIAMICNEERKIYGMQFHPEVYHTTDGIALLSNFIKIAGCKTDWTMNSFLDEQQRVIKKQVGDKKVIAAISGGVDSSVAAALTYRAIGDQLHCIFIDNGLLRYNEAEKISQLLVNQFQMPITIVDKSSVFLNKLKSITDPEQKRKIIGKTFIEVFEGEANKIGGVEFLMQGTIYPDVIESGGAVGKESVTIKSHHNVGGLPDVMRLQLVEPLKLLFKDEVRLLGKELGISDEILMRHPFPGPGLAIRIIGEVTQEKIKMLQAADDIYINLIKKYNLYNIIWQAFAVILPVKTVGVMGDSRTYGYTCALRAVTSSDGMTAGCFPFGVDTDTKLIFYEFLQEVSNAIVNNVDGINRVVYDTTSKPPATIEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT46</accession>
    <name>Q3YT46_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">secF</name>
        <name evidence="11" type="ordered locus">Ecaj_0058</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a complex with SecD. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304187.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0058"/>
    <dbReference type="EnsemblBacteria" id="AAZ68109">
        <property type="protein sequence ID" value="AAZ68109"/>
        <property type="gene ID" value="Ecaj_0058"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0058"/>
    <dbReference type="eggNOG" id="COG0341">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050012_1_1_5"/>
    <dbReference type="OMA" id="FMAIVVY"/>
    <dbReference type="OrthoDB" id="410730at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_B">
        <property type="entry name" value="SecF_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005665">
        <property type="entry name" value="SecF_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01755">
        <property type="entry name" value="SECFTRNLCASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00916">
        <property type="entry name" value="2A0604s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00966">
        <property type="entry name" value="3a0501s07"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="293" mass="32501" checksum="B528DF04B55ACD8C" modified="2005-09-27" version="1">MIFRNGTNINFSKYRMCAMFCSGVLMLISILLVLFKGVCFGTDFAGGIVIELRASQIDTKNVTLKLQEKGFVGFSVQSFRNNDKELMIRFKSGKDVNHTDTIEKIKLALGEILGEVTYLKIDYVGPQVGYKQIFEGLCAIIFSLLGMFLYLWVRFQWRFALGGVLALIHDVILTLGLISLCNIEFNLSSTAAILTVIGYSVNDSVVIYDRIRELLRKKLQKKLSDIINVSINATLSRTILTSGTTMLASLPLILLCQGTVRELSIIVFFGIAVGTYSSIFIATASLSNNVIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT47</accession>
    <name>Q3YT47_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endonuclease distantly related toarchaealHolliday junction resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0102 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304186.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68108">
        <property type="protein sequence ID" value="AAZ68108"/>
        <property type="gene ID" value="Ecaj_0057"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0057"/>
    <dbReference type="eggNOG" id="COG0792">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115353_0_4_5"/>
    <dbReference type="OMA" id="PITYKQQ"/>
    <dbReference type="OrthoDB" id="2044249at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003509">
        <property type="entry name" value="UPF0102"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34039">
        <property type="entry name" value="PTHR34039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02021">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="122" mass="14716" checksum="639768C51DA363A7" modified="2005-09-27" version="1">MQIKKNYLKVTYNMIGYLGEVIIIWILKCKRYHIIKHRYKCTLGEVDIIAFKNQTLVFIEVKTSILRSEIPITYKQQQSIIRVAKHFITYYTKFEKYNIRFDLYFLSLSKGLIHITNAWQET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT62</accession>
    <name>Q3YT62_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phospholipid/glycerol acyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.51" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304171.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0042"/>
    <dbReference type="EnsemblBacteria" id="AAZ68093">
        <property type="protein sequence ID" value="AAZ68093"/>
        <property type="gene ID" value="Ecaj_0042"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0042"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027938_5_1_5"/>
    <dbReference type="OMA" id="MPRPLCY"/>
    <dbReference type="OrthoDB" id="1756450at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="PlsC" evidence="2">
        <location>
            <begin position="67"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="240" mass="27299" checksum="A483AD48A15AE8BB" modified="2005-09-27" version="1">MLFYILSVLFTILHAITILPLYLIFPMSLKIKCSVYGIKVILFLCKTIDKVDYEIRGYENLPDDSSYIIASEHQSPLETLILFTTLKDPVYVLKKELRYLPFFGLYFMLIKMIFINRQQKIQALKHMLSATKSRVKEGRTVIVFPQGSRTIPGEKISIKPGITAIYNQLSIPIVPVAVNTGLFWPASILSFKKRPGKAIIQILPPIYPGLSKQEFTNELETRISTASNSLIKEALQQNSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT63</accession>
    <name>RS20_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsT</name>
        <name type="ordered locus">Ecaj_0041</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304170.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT63"/>
    <dbReference type="SMR" id="Q3YT63"/>
    <dbReference type="STRING" id="269484.Ecaj_0041"/>
    <dbReference type="EnsemblBacteria" id="AAZ68092">
        <property type="protein sequence ID" value="AAZ68092"/>
        <property type="gene ID" value="Ecaj_0041"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0041"/>
    <dbReference type="eggNOG" id="COG0268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160655_3_0_5"/>
    <dbReference type="OMA" id="KAESNIH"/>
    <dbReference type="OrthoDB" id="2005443at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00500">
        <property type="entry name" value="Ribosomal_S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002583">
        <property type="entry name" value="Ribosomal_S20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036510">
        <property type="entry name" value="Ribosomal_S20_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398">
        <property type="entry name" value="PTHR33398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01649">
        <property type="entry name" value="Ribosomal_S20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46992">
        <property type="entry name" value="SSF46992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00029">
        <property type="entry name" value="S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000224966" description="30S ribosomal protein S20">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00500"/>
        </source>
    </evidence>
    <sequence length="95" mass="10987" checksum="C9877452F10B47B1" modified="2005-09-27" version="1">MANHPSAKKMIKVIKKRTMVNRMRKSRAHNYVKKFLLALAAGNKELMVETFKKAESNLHKCVNKKIIHRNTAARKISRMALKLKTFDLQQQAKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT65</accession>
    <name>Q3YT65_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0039</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0039"/>
    <dbReference type="EnsemblBacteria" id="AAZ68090">
        <property type="protein sequence ID" value="AAZ68090"/>
        <property type="gene ID" value="Ecaj_0039"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0039"/>
    <dbReference type="eggNOG" id="COG1230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_0_0_5"/>
    <dbReference type="OMA" id="GHEKMLH"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="312" mass="34638" checksum="EC4FADAFE39ADE14" modified="2005-09-27" version="1">MECGYKMSLIHKDKTSSHENKLIYAILIIIITMVTEVIGGIISNSLALLSDAGHMFTDFISLLLSWLAYKVAMKKSDACRSYGYHRFQVVAAFINGLTLFGIAILIILESIKRFVSPEKVCWEIMMSVAVLGLVANVVSFFLLYRKNENNLNLKSAVLHVIGDLLGSVAAIIASVVIMFTSWEIVDPLLSVFVSIIILGGAYRIIKNSGHILLEGTPDNVNPDKIRTSVCENIPGVLDVHHIHIWSLTTDHPIMTMHIKLDKAIVDNSLKYSQVVVSVKKLLSENFNIIHVTIEAEYDNCADDSVMIEIAHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT74</accession>
    <name>Q3YT74_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Znf/thioredoxin_put domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304159.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0030"/>
    <dbReference type="EnsemblBacteria" id="AAZ68081">
        <property type="protein sequence ID" value="AAZ68081"/>
        <property type="gene ID" value="Ecaj_0030"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0030"/>
    <dbReference type="eggNOG" id="ENOG5032ZVA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243707_0_0_5"/>
    <dbReference type="OMA" id="DYKFEHI"/>
    <dbReference type="OrthoDB" id="1043256at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021834">
        <property type="entry name" value="DUF3426"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011723">
        <property type="entry name" value="Znf/thioredoxin_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11906">
        <property type="entry name" value="DUF3426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13717">
        <property type="entry name" value="zinc_ribbon_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02098">
        <property type="entry name" value="MJ0042_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Znf/thioredoxin_put" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="221" mass="25687" checksum="2BC25751B6BFB8A1" modified="2005-09-27" version="1">MRIECKNCKAVYRVDSSKFPTEGKKVRCTHCNNTWTHIPSEDKAEDYPEKNYTNKESVVNPNLQASKNPTQHHSSTRHSFLKKTLSVCTTILLIFSLSVMLQNNIPYKFRKIYRIIEMYDTTNIQLADSKIKILKKDEYNIAIKIEGIIKNQSDQERFIPGIQLVLYNKQKKAVASKKLNQHKTDIIPSKQDYKFEHIIHAPKDTDSIQIKIGNLFEIAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT79</accession>
    <name>Q3YT79_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0025"/>
    <dbReference type="EnsemblBacteria" id="AAZ68076">
        <property type="protein sequence ID" value="AAZ68076"/>
        <property type="gene ID" value="Ecaj_0025"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0025"/>
    <dbReference type="HOGENOM" id="CLU_1658086_0_0_5"/>
    <dbReference type="OMA" id="CIMYIMI"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="194" mass="22611" checksum="FB21C056984CBADA" modified="2005-09-27" version="1">MASTVINKTLYQFLRSINFYTIHSYVIMLFNNEQVFMKLKETTINGMLVLCFAPMVAFYHKMKVLFTCGLPLLDGTEESNYEHAVMIGCLSTVLCCVIYIMILIHFNIKLMLFLLSKITGKNFNALNDIISRFIDVSVIHFVISNIHLKFLKCISHKNKSEDKQIYKDFAKKTNIIIKSIEHHRKLYENFILPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT91</accession>
    <name>Q3YT91_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage portal protein, HK97 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304142.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0013"/>
    <dbReference type="EnsemblBacteria" id="AAZ68064">
        <property type="protein sequence ID" value="AAZ68064"/>
        <property type="gene ID" value="Ecaj_0013"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0013"/>
    <dbReference type="eggNOG" id="COG4695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054516_0_0_5"/>
    <dbReference type="OMA" id="YFVKFNV"/>
    <dbReference type="OrthoDB" id="389520at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006944">
        <property type="entry name" value="Phage/GTA_portal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006427">
        <property type="entry name" value="Portal_HK97"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04860">
        <property type="entry name" value="Phage_portal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01537">
        <property type="entry name" value="portal_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="393" mass="44881" checksum="2299717E2FC29A79" modified="2005-09-27" version="1">MHFFKKKSIKDYSYTFSIPIQLFTEAVWKNRSYANFAENGYIKNVIAFRSIHMIASAAASVPLILNKTVRNNTFQIKNHPLLKLISRPNNTTSKSEFIEGILTYKLISGNAYILMIENHSMIPKELHLLRPDRVEIISDKDNRPYSYRYSINSCSYDYKINKLTNYSQILHIKNFHPLNDYYGLSPIEAASYSIDQHNQAGSWNQAMLQNGARPSGALIVNTKGNGNGSLTQEQYKRLKSQVDEFYSGPRNAGRPILLEGGLEWKEMSLSPKDMDFIESKHSSARDIALAFGVPPQLLGIPGDNTYSNLIEARLSLWEQTILPHLDNIISHLNNWLTPKFGHNIFLSYDKDSISVLTEKRKQLWQYVEHATFMTINEKRAAFGLPPIENGNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YTA2</accession>
    <name>Q3YTA2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304131.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68053">
        <property type="protein sequence ID" value="AAZ68053"/>
        <property type="gene ID" value="Ecaj_0002"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0002"/>
    <dbReference type="HOGENOM" id="CLU_121355_0_0_5"/>
    <dbReference type="OMA" id="FLSNGFW"/>
    <dbReference type="OrthoDB" id="2019385at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="186" mass="22646" checksum="318A2A2C4271CE6E" modified="2005-09-27" version="1">MLFYRYMLFLFLLKNIKNLVVRVFFLALFLYFSSFIFIAMKEGIKVAFAYKDPQSVAIYIYKYLKWCYYNYDSLTYSVFFKAGVSLIIPFWFYLKVSKINWNEFFRCLFEYVCSMFKKDNYNKENLEFYNYNDFNFIDTNHVEEIDKIKKNAIAEIEESINKMVSNILCVKSKKNNQNYDNVIDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQN5</accession>
    <name>Q3YQN5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glyceraldehyde-3-phosphate dehydrogenase, typeI</fullName>
            <ecNumber evidence="9">1.2.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0939</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305043.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0939"/>
    <dbReference type="EnsemblBacteria" id="AAZ68970">
        <property type="protein sequence ID" value="AAZ68970"/>
        <property type="gene ID" value="Ecaj_0939"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0939"/>
    <dbReference type="eggNOG" id="COG0057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030140_0_2_5"/>
    <dbReference type="OMA" id="NAKVLAW"/>
    <dbReference type="OrthoDB" id="944149at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43148">
        <property type="entry name" value="PTHR43148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="8">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="209"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="6">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="333" mass="36957" checksum="B1BB5C3743ED846A" modified="2005-09-27" version="1">MKIGINGLGRIGRCLIRAIYENTQSSIELSALNGSTSSEIHAHLIQYDSVHGKFPYNVESEEGYIIINDKKIPLSIEKEPKNIFWEKHNIDIVLECTGKFNKKSLAIQHINSTVKKVIVSAPVQDSDVTIVYGVNNEMLKKEHNVISAGSCTTNCIAPILKIMNDTVGIKNGFITTVHSYTNDQNLVDNNHKDLRRARACMMSIIPTTTGATKTIDLIIPELKGKLNGTAIRVPTPNVSMIDLVFNSVKSTNANEINDIIKNSSQNSKVISITDKKLVSIDFCHSTYSSIVDANETCVTDNNLCRIAAWYDNEWAFAMRMLDIVSLLSELIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQN7</accession>
    <name>Q3YQN7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0937</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305041.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0937"/>
    <dbReference type="EnsemblBacteria" id="AAZ68968">
        <property type="protein sequence ID" value="AAZ68968"/>
        <property type="gene ID" value="Ecaj_0937"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0937"/>
    <dbReference type="eggNOG" id="COG0670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058671_1_1_5"/>
    <dbReference type="OMA" id="SHNAYAE"/>
    <dbReference type="OrthoDB" id="1947455at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="PTHR23291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="234" mass="26140" checksum="029D9AF8872F42FA" modified="2005-09-27" version="1">MDNYSNVRFQSAYYSVGLRNYLVKVYNYMAMALGLTGIVAFFVSSSSALMSAIYNTPLHWVVMFAPIGLVFLMSYKLNVFSFQTVLTVFFSFAALMGVSISYIFLVYTSASIAKVFFISSSMFGIMAWYGNVTKKDLSQFGTFLFMGLVGIVLASLVNLFLHSGPLHFAISIVAVVLFTGMTAYDAQRIKDVYYKFNDGTDVSVNKMAILGATTLYFNYVNIFLSLLNLQGERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQP5</accession>
    <name>Q3YQP5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable chromosome-partitioning protein ParB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0929</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in chromosome partition. Localize to both poles of the predivisional cell following completion of DNA replication. Binds to the DNA origin of replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305033.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0929"/>
    <dbReference type="EnsemblBacteria" id="AAZ68960">
        <property type="protein sequence ID" value="AAZ68960"/>
        <property type="gene ID" value="Ecaj_0929"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0929"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023853_0_0_5"/>
    <dbReference type="OMA" id="IALSYQR"/>
    <dbReference type="OrthoDB" id="653368at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041468">
        <property type="entry name" value="HTH_ParB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004437">
        <property type="entry name" value="ParB/RepB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17762">
        <property type="entry name" value="HTH_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00180">
        <property type="entry name" value="parB_part"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="5">
        <location>
            <begin position="34"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="283" mass="32104" checksum="EE10B172BD9228FB" modified="2005-09-27" version="1">MTKRLGKGIFELIGNSNLEINNNTSNNINTSNHLTMPINLLNPSTSQPRKTFDQASLKELAESISKHGIIQPIIIRKNPHKKGYEIIAGERRWRASILAKLKSVPVIVKEISDSQCLELSIIENIQRQDLTPIEEAEAYSNLINTFSYTHEDLASILGKSRSHITNMIRILSLPPSIKSMVNDKLISFGHARALINIENPEEIAQKIISSNLNVRQTELLIKNLQKPHRNKILENFTELENSLSEMLKLKIKIKGLNHKGNIVIKYNNQQQFNSILDKLKNPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQR8</accession>
    <name>Q3YQR8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305013.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0906"/>
    <dbReference type="EnsemblBacteria" id="AAZ68937">
        <property type="protein sequence ID" value="AAZ68937"/>
        <property type="gene ID" value="Ecaj_0906"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0906"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="YSENFRI"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230953" evidence="1">
        <location>
            <begin position="25"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="281" mass="31525" checksum="DE94CF01CD24D967" modified="2005-09-27" version="1">MNNKRNFFLIGMSLLINLLLPIDASSMEVHNYTHFKPRLYISGQYRPGVSHFSKFSVKETHCNTVQLVGLTKDIKVTNNSSINTNTSFNFPYVAEFQDNAMSFSGAIGCFYSENFRIEVEASYEEFDVKNPEGSTTDSYRYFALARGMDGNNIPTSQKFTVMRNDGLLISSVMINGCYNVILNDIQAEPYICAGLGGDFIEFFNGFHVKLAYQGKVGISYQIFPEVRLFIDGYYHKVKGNKFKNLHVQHVGALAALPKVTSAVATLNIGYFGCEAGVRFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQT0</accession>
    <name>Q3YQT0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305001.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0894"/>
    <dbReference type="EnsemblBacteria" id="AAZ68925">
        <property type="protein sequence ID" value="AAZ68925"/>
        <property type="gene ID" value="Ecaj_0894"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0894"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_26_0_5"/>
    <dbReference type="OMA" id="IQLMRAF"/>
    <dbReference type="OrthoDB" id="1288157at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="85"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="215" mass="24809" checksum="613AAE656304A9F1" modified="2005-09-27" version="1">MSIHAKNKQNKPEYANEIKAHDPLIEDLIEKNIQQHKFTGEGIRLDVDDYASKNFKKEGAESTKELKSATKVRPHPVDECVGKEIKRQRIMRGMSQNQLANKLGITFQQVQKYEKGTNRIVISRLYQLASVLNVEVRDIMLKLQEDLKNISCDNPITPPHALRDNEEKFLPEFNDSKIDSKEVLMMVRAYTCIKNEKVRNIIYNLVKALSLDNKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQT1</accession>
    <name>NDK_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="1">NDK</shortName>
            <shortName evidence="1">NDP kinase</shortName>
            <ecNumber evidence="1">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ndk</name>
        <name type="ordered locus">Ecaj_0893</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDK family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAZ68924" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68924.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YQT1"/>
    <dbReference type="SMR" id="Q3YQT1"/>
    <dbReference type="STRING" id="269484.Ecaj_0893"/>
    <dbReference type="PRIDE" id="Q3YQT1"/>
    <dbReference type="EnsemblBacteria" id="AAZ68924">
        <property type="protein sequence ID" value="AAZ68924"/>
        <property type="gene ID" value="Ecaj_0893"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0893"/>
    <dbReference type="eggNOG" id="COG0105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060216_8_1_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006165">
        <property type="term" value="P:nucleoside diphosphate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="SSF54919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDP_KINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000226561" description="Nucleoside diphosphate kinase">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="141" mass="15943" checksum="E326EA5B4AEC485A" modified="2006-12-12" version="2">MLERTLSILKPDVVKRNITGQVNSYIENSGLKIVTQKMCLLTRFQAEEFYAIHKSQHFFIPLVDFMVSGPIIVQVLEGENAISLYRELMGATDPKKANPGTIRGDFAENIDANCVHGSDSLDNAVREIRFFFSDYELLSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQU6</accession>
    <name>Q3YQU6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA(Ile)-lysidine synthase</fullName>
            <ecNumber evidence="6">6.3.4.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile)-2-lysyl-cytidine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile)-lysidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tilS</name>
        <name evidence="8" type="ordered locus">Ecaj_0878</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP + diphosphate + H(+) + lysidine(34) in tRNA(Ile2)</text>
            <dbReference type="Rhea" id="RHEA:43744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10670"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83665"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the tRNA(Ile)-lysidine synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.19" evidence="6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0878"/>
    <dbReference type="EnsemblBacteria" id="AAZ68909">
        <property type="protein sequence ID" value="AAZ68909"/>
        <property type="gene ID" value="Ecaj_0878"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0878"/>
    <dbReference type="eggNOG" id="COG0037">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018869_3_2_5"/>
    <dbReference type="OMA" id="YKRTLYR"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01992">
        <property type="entry name" value="PP-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01161">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012795">
        <property type="entry name" value="tRNA_Ile_lys_synt_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="PTHR43033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02432">
        <property type="entry name" value="lysidine_TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="ATP_bind_3" evidence="7">
        <location>
            <begin position="19"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="24"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="433" mass="50163" checksum="581938C3386A4BD4" modified="2005-09-27" version="1">MRDLKLLFQKKVQNLNSAYAVAVSGGIDSMVLLHLSAQHRNHNTPIILTVNHGLRQEAKQEALFVFQHSQNLNLKCHILNWHGKLPESNIQSSARQIRYGLLLQWCNENRINYLMVAHQKNDQAETIMIRLERGSGLDGLAGMQECTYLNGICILRPLLSVSRTELLQYASQNNIPWINDPSNNNKKYKRTLYRNMLETTDNPEILINRLYTASTHIKRSLDCILHYVREAIDECLEFTNLGYINIKLDVFLNLPEEISLRLLTYSIMTIGQQKYKPRYKKINKIFYKIQNNELNSAQTLCSCKIVKNQNNTISIIREVSTIKELIVNYPTNTSIIWDNRFKISIAHHDANSLIICSLNDSNIPDDLKKLNREAVRCLPVLKHQNKIVAYPLQNNNKNDYIDTKSYIRIEEVLVKQNLINLTYSEFISKEPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQU7</accession>
    <name>Q3YQU7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0877</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304984.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0877"/>
    <dbReference type="EnsemblBacteria" id="AAZ68908">
        <property type="protein sequence ID" value="AAZ68908"/>
        <property type="gene ID" value="Ecaj_0877"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0877"/>
    <dbReference type="eggNOG" id="COG0168">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030708_0_1_5"/>
    <dbReference type="OMA" id="GSMNHFS"/>
    <dbReference type="OrthoDB" id="756220at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="483" mass="54073" checksum="41854075C6F95C7C" modified="2005-09-27" version="1">MYKSLRSIIFITGVFSLVFAFLMIIPTIVNYYTGYVWQHFFCSCIFVVLFGLVCILLGQPSNLKQMSVVIVTVVIWIVLSAISAIPFVCDSDLHYVDAFFEAVSGLTTTGATVLTDLQNQSPGILIWRALLNAIGGLGIITSGIFLFSYLKICNLHELYHSESSDQSKKFKYGIVRSSMYIFIMYCMLMTSCAILYWIFGMSGFDAICHAMSTVSTGGFSSYDDSLQHFNNVYIEIVAVMFMLLSSCPFIVYLKIVARQCFYDEQVVFFAVIVFLFVMISVMNCYFDEIMSGHSFFDILRYSVFSVVSLSTSTGFVNYDYTNWHFFSTFGILMMLIGGCSGSTNSGIKVYRIVLLGKALHAYISSIVYPTQISTVKYNRKRVDEDFIRNIGLFFSLYIFLLFIGSAIVSSGGVDFITAFSAVSGAFSNTGPGIGHIVGPSANYYNLLPSVKVLLSFLMLMGRLEIIPFFSCMYLMTNVLLKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQV4</accession>
    <name>Q3YQV4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pentapeptide repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0870"/>
    <dbReference type="EnsemblBacteria" id="AAZ68901">
        <property type="protein sequence ID" value="AAZ68901"/>
        <property type="gene ID" value="Ecaj_0870"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0870"/>
    <dbReference type="eggNOG" id="COG1357">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026220_0_0_5"/>
    <dbReference type="OMA" id="KIDQANF"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13599">
        <property type="entry name" value="Pentapeptide_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="635" mass="71291" checksum="496B5A85D9772419" modified="2005-09-27" version="1">MILITYNQLDYIIRLYKYNMKHYIKYIFTVLTTCALFFNINICYANATDQNQQQGDITNQENFFTVKRTSLTKEDFVSFIIKCHAKGIPLDFKKEFGNNLSGADFSDLDLRGSVFNNVNLLHANFTRANLSSSTFTDTNMQGASFINANLSRSNIKNSNLNFANFTSADLQKTIITQSKINNTNFSDVDMRFSILTGIDGSFANFSEAELTLVSILNSRIEKTNFHDVEGESILIQDCNLSNSNFFGANLHNSKIQFSNLTNTILYGVNLDSSDVTGSNFSNSNMEVSNISFSNFQNADLSNTNMHLVDAHYTNLNNTNLHKVCMSNGTLVGVNLKNSTLSNADLSNANLINSIMEESDMQEVLFNNTHVTKSNMKYAILNNTTINNTVFLNSDLTMTSIRNTEWHNSSIYSSKLTSAIIDNNTIKDSTYDKVTAANTIWSNSTIENSNISHTNLDFSTFNNNKIHKVSFFSNHLESAKIENSNITGSSIYKNILTNFLTNNCDTENTIIIENKNFILTALPSTAITSLKSLQSTITNNKKFDVNFSNFDFRKINLNNSNFSNSILKNANFSGLKLENVNFSFADLQSSHFKNSQLKNVDFSNANLENADLSHSKLNEVNTHKTNLRNVKTDLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQV8</accession>
    <name>Q3YQV8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bifunctional purine biosynthesis protein PurH</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="9">Phosphoribosylaminoimidazolecarboxamide formyltransferase</fullName>
                <ecNumber evidence="9">2.1.2.3</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="9">AICAR transformylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">IMP cyclohydrolase</fullName>
                <ecNumber evidence="9">3.5.4.10</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="9">ATIC</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="9">IMP synthase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="9">Inosinicase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="9" type="primary">purH</name>
        <name evidence="11" type="ordered locus">Ecaj_0866</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>H2O + IMP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">The IMP cyclohydrolase activity resides in the N-terminal region.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the PurH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.3" evidence="9"/>
    <dbReference type="EC" id="3.5.4.10" evidence="9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304974.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0866"/>
    <dbReference type="EnsemblBacteria" id="AAZ68897">
        <property type="protein sequence ID" value="AAZ68897"/>
        <property type="gene ID" value="Ecaj_0866"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0866"/>
    <dbReference type="eggNOG" id="COG0138">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016316_5_2_5"/>
    <dbReference type="OMA" id="WRVAKFV"/>
    <dbReference type="OrthoDB" id="1049662at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="PTHR11692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MGS-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="504" mass="55781" checksum="91F3F2D6CA4861C1" modified="2005-09-27" version="1">MKVKRAIISVYNKTNIIELAKFLIAQKVEIIATSSTYQTLLGAELQVTEVSDYTNFPEIMGGRVKTLHPKIHGGILSNRNNHATECSNLGIYDIDLVIVNLYPFFQVASLKTSTESEIIEKIDIGGITLLRAAAKNFHNVTVVSDINDYETLKAEMTNNQNTTTLTYRKHLAKKAFAITSSYDSNIYNWMNKDSNDTLPKTFIIHGNKVQTLRCGENPHQQGAFYSSAKNKYPLEQLHGKELSYNNIVDIESAINIVSEFTQPAATIIKHSNPCGTAIADNITSAYNNAFSCDTKSSFGGIVALNREINEDIAEEINKIFIEVIVGTSITDKALEIIQKKKNVRVILSTQYNSPKYTIKNVSDGFLLQESNTNQLSAKDLIQVTNFPVSDDTISNLLFAWKVCKHVKSNAIVIAKDHRTIGVGAGQMSRVDSLEIAIKKAQDCTGAVLASDAFFPFTDSILLSASVNISAIIQPGGSLKDKEVIEEANNQKIAMFFTNIRNFYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQX0</accession>
    <name>Q3YQX0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0854</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304962.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0854"/>
    <dbReference type="EnsemblBacteria" id="AAZ68885">
        <property type="protein sequence ID" value="AAZ68885"/>
        <property type="gene ID" value="Ecaj_0854"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0854"/>
    <dbReference type="eggNOG" id="COG0695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_7_3_5"/>
    <dbReference type="OMA" id="LLWWKGV"/>
    <dbReference type="OrthoDB" id="2045232at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097573">
        <property type="term" value="F:glutathione oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03418">
        <property type="entry name" value="GRX_GRXb_1_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011900">
        <property type="entry name" value="GRX_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02181">
        <property type="entry name" value="GRX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="6">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="81" mass="9360" checksum="08793DF14DFA97FB" modified="2005-09-27" version="1">MTKVIIYTKDPCPYCTKAKALFNKKNILFKEIDVTNNSTLLEEMIQKSNGMRTLPQIFINDQHIGGCDDLYRLYESGKLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQX8</accession>
    <name>Q3YQX8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304954.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YQX8"/>
    <dbReference type="STRING" id="269484.Ecaj_0846"/>
    <dbReference type="EnsemblBacteria" id="AAZ68877">
        <property type="protein sequence ID" value="AAZ68877"/>
        <property type="gene ID" value="Ecaj_0846"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0846"/>
    <dbReference type="eggNOG" id="COG2834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055272_6_0_5"/>
    <dbReference type="OMA" id="LLKWDYY"/>
    <dbReference type="OrthoDB" id="1933827at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd16325">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029046">
        <property type="entry name" value="LolA/LolB/LppX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004564">
        <property type="entry name" value="OM_lipoprot_carrier_LolA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35869">
        <property type="entry name" value="PTHR35869"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03548">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89392">
        <property type="entry name" value="SSF89392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="193" mass="22195" checksum="628122E41C8A5788" modified="2005-09-27" version="1">MKISVVYIFFFIVCFFSVHSYSSEKIKVEALSYFNTIHSFKAEFVQTNSANNVAQYGTLIMKKPGLLKWDYYAPTPASIIIQGTTISYYDKELEEYSYAIVNNPIINLLSSDIKNIEDIIFTNISTVDNNKIITLQDKKTQILAAVVFNVNPITIVGLNIINPDSTIYIKFYNIQNNITIEESEFKHNTFYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQY1</accession>
    <name>Q3YQY1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein HflK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0843</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">HflC and HflK could encode or regulate a protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">HflC and HflK may interact to form a multimeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the band 7/mec-2 family. HflK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304951.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0843"/>
    <dbReference type="EnsemblBacteria" id="AAZ68874">
        <property type="protein sequence ID" value="AAZ68874"/>
        <property type="gene ID" value="Ecaj_0843"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0843"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039173_0_1_5"/>
    <dbReference type="OMA" id="WIVQYRI"/>
    <dbReference type="OrthoDB" id="577287at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03404">
        <property type="entry name" value="SPFH_HflK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010201">
        <property type="entry name" value="HflK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF2">
        <property type="entry name" value="PTHR43327:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01933">
        <property type="entry name" value="hflK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="7">
        <location>
            <begin position="70"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="355" mass="40417" checksum="0A1D4FEDE81766AA" modified="2005-09-27" version="1">MFDDHDPWDSNNKEENQAKGYKNSNDINKIINRLNNTLNSFLHNKKKNNSYGKIQFIVAFLVIISLYMASGFYMVEPEEEAVELLFGKYHNTVGPGLRYHFPSPIGQIIKLKVKTINREEIGSKLYTDSTSDHGEGVMLTGDENIVNINFDVHWRINNAYNYLFKVRDNQVGDTVKNAAESAMREVIGKSSISFAIEGKGRAVISQETKTLLQNILDHYEMGVEVLSIQLKKVDPPEKVISSFRDVQSARADKEKLINEAYAYRNQVLPRAKGEAIKIKLDAEAYESEVVNTAEGNAKRFTALYNEYVQQPDAVRNRLYLETMEEILNKNDKVVVSDDLKGMLSYFPLADPKNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQY7</accession>
    <name>Q3YQY7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304945.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68868">
        <property type="protein sequence ID" value="AAZ68868"/>
        <property type="gene ID" value="Ecaj_0837"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0837"/>
    <dbReference type="HOGENOM" id="CLU_803479_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="345" mass="39786" checksum="1AF3E7E33EB8D273" modified="2005-09-27" version="1">MYDKTVLKIFTDVGINDDKDTIVLLIDAIIFNAFIVNGVLNDNGLNMLIEELGKRYNTDNSNVFFPVSAIFKLKYDTEGMPYVHITNIVVLWSCMLTILPKKLLTEQNFVNFVKLTLSRSLDSEYLDPCNQLFSEFVTYYYKVGKNGNLPEKVVNHLNAIKQGKICAQLSALSEFPKFTSMLKEYVDLLQYLIRETFVDGEASNPAYWLKPLKTPYDFISDFTYQPKVYRIFADNGIAVKEGDVIALFHAAMLGATIDHVDFLRRNTPQTIASLIHDMYRFRYYKSFGFYYYESMLSLVKQMKAGGKIIPLAYYAFSIMGWDVQKFRDFAEYVSGISSQSYEKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-04-07" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR02</accession>
    <name>Q3YR02_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rplM</name>
        <name evidence="5" type="ordered locus">Ecaj_0822</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0822"/>
    <dbReference type="EnsemblBacteria" id="AAZ68853">
        <property type="protein sequence ID" value="AAZ68853"/>
        <property type="gene ID" value="Ecaj_0822"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0822"/>
    <dbReference type="eggNOG" id="COG0102">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082184_2_2_5"/>
    <dbReference type="OMA" id="DFVVIIN"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_L13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="156" mass="17709" checksum="AAAFC1EA7371258A" modified="2005-09-27" version="1">MQMETFSLKASQIKKRWVVIDASGVVVGRLAAFVANILRGKDKPEYTPHMDCGDNVIIINAEKVQFTGSKLKGKVYYRHTGYPGGLKSCTPADLLLNKHPERIINMAIRRMLSTGPMARRRLKNLYIYSGPEHKHNAQQPVKLDFLSMNAKNNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR10</accession>
    <name>Q3YR10_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Sec translocon accessory complex subunit YajC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">yajC</name>
        <name evidence="13" type="ordered locus">Ecaj_0814</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">The SecYEG-SecDF-YajC-YidC holo-translocon (HTL) protein secretase/insertase is a supercomplex required for protein secretion, insertion of proteins into membranes, and assembly of membrane protein complexes. While the SecYEG complex is essential for assembly of a number of proteins and complexes, the SecDF-YajC-YidC subcomplex facilitates these functions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">Part of the SecDF-YidC-YajC translocase complex. The SecDF-YidC-YajC translocase forms a supercomplex with SecYEG, called the holo-translocon (HTL).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the YajC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0814"/>
    <dbReference type="EnsemblBacteria" id="AAZ68845">
        <property type="protein sequence ID" value="AAZ68845"/>
        <property type="gene ID" value="Ecaj_0814"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0814"/>
    <dbReference type="eggNOG" id="COG1862">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116157_2_0_5"/>
    <dbReference type="OMA" id="HMSANTQ"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003849">
        <property type="entry name" value="Preprotein_translocase_YajC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33909">
        <property type="entry name" value="PTHR33909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02699">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01853">
        <property type="entry name" value="YAJCTRNLCASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01323">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00739">
        <property type="entry name" value="yajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0811">Translocation</keyword>
    <keyword evidence="9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="148" mass="15668" checksum="814B689E1E1B415C" modified="2005-09-27" version="1">MLTINTILDKVGPLIGPLVLKMSLISEVFAAATTTAPASGVGSSIAGLVPLVLIFFVFYFFIIRPQQKKIKEHNKLLDSIKKGDKVIISSSILGSVTKVDATNGHFLVEISEGVEIKVLKSSISEVLNKNNKPTSSAVEGSPSVSSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-08-12" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR23</accession>
    <name>Q3YR23_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heat shock protein DnaJ, N-terminal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0801</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0801"/>
    <dbReference type="EnsemblBacteria" id="AAZ68832">
        <property type="protein sequence ID" value="AAZ68832"/>
        <property type="gene ID" value="Ecaj_0801"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0801"/>
    <dbReference type="eggNOG" id="COG2214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_13_5_5"/>
    <dbReference type="OMA" id="SIWMNIN"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="90"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="140" mass="15816" checksum="A0F92B10CB3A5FEC" modified="2005-09-27" version="1">MQKNTFNLYNMDIPCLNQVIYMLFIFLIIFSIIGVIFVLPILALFIFLILKRNSIINMSEVMTSTFNKVMNEASMYSNTRSTGNSLSKAEALEILGLEKNASPEQINTAYHKLMKSMHPDKGGSPYLAQKLNEARDTLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR24</accession>
    <name>Q3YR24_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogen-fixing NifU, C-terminal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304908.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0800"/>
    <dbReference type="EnsemblBacteria" id="AAZ68831">
        <property type="protein sequence ID" value="AAZ68831"/>
        <property type="gene ID" value="Ecaj_0800"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0800"/>
    <dbReference type="eggNOG" id="COG0694">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060555_0_2_5"/>
    <dbReference type="OMA" id="AIMEHYM"/>
    <dbReference type="OrthoDB" id="2010888at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035433">
        <property type="entry name" value="NFU1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036773">
        <property type="entry name" value="HIRIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="SSF110836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nfu_N" evidence="1">
        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="186" mass="20609" checksum="D6ED7D19FB64858F" modified="2005-09-27" version="1">MFIQIEETPNPNTLKFMPGVPVNNGKVSEFADNIAAESSPLAAALFKVEHVKGVFFGGDFVSITKSDEIEWDVLKPEVLTVIMEFLTLNPGDSTEDFNEEETDEFFDEKDVEMVDKIKELINDYVKPAVMQDGGDIKFKGYSNGIVFVKLRGACSGCPSASITLKEGIYNMLSYYIPDIQGVESVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR33</accession>
    <name>Q3YR33_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ETC complex I subunit conserved region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0791</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304899.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0791"/>
    <dbReference type="EnsemblBacteria" id="AAZ68822">
        <property type="protein sequence ID" value="AAZ68822"/>
        <property type="gene ID" value="Ecaj_0791"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0791"/>
    <dbReference type="eggNOG" id="ENOG5032RJS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077196_4_1_5"/>
    <dbReference type="OMA" id="MIARIYS"/>
    <dbReference type="OrthoDB" id="2028651at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038532">
        <property type="entry name" value="NDUFS4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219">
        <property type="entry name" value="PTHR12219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04800">
        <property type="entry name" value="ETC_C1_NDUFA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="97" mass="11408" checksum="112B856DB3A26836" modified="2005-09-27" version="1">MRARIYKPTKSAMQSCDNKQKKWKLEFEPSCTQYIEPLMKWTGSHDTRQQIRLFFKTKELAIAYAIAHNIDYIVLQNNSRTTSAKSYASNFTRLRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR37</accession>
    <name>Q3YR37_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conjugal transfer protein TrbG/VirB9/CagX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0787</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrbG/VirB9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0787"/>
    <dbReference type="EnsemblBacteria" id="AAZ68818">
        <property type="protein sequence ID" value="AAZ68818"/>
        <property type="gene ID" value="Ecaj_0787"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0787"/>
    <dbReference type="eggNOG" id="COG3504">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058585_3_0_5"/>
    <dbReference type="OMA" id="GFYGYQS"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06911">
        <property type="entry name" value="VirB9_CagX_TrbG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010258">
        <property type="entry name" value="Conjugal_tfr_TrbG/VirB9/CagX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033645">
        <property type="entry name" value="VirB9/CagX/TrbG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038161">
        <property type="entry name" value="VirB9/CagX/TrbG_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03524">
        <property type="entry name" value="CagX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="270" mass="30953" checksum="5DE8A6838E69AA2E" modified="2005-09-27" version="1">MLYILLHTQMRNTAIVFVLILLSSTICFAKQEVRSIAQDNHIKVMTYNPHAIHKYTGFYGYQSSILFEEGETIDTISMGDSTGWQLMPKGNRLFIKPVADKADTNATIITNKRVYYFEFFAEEAKGLDDPRLAYEVRFIYSSTENLVGEDSSGMIFPVSQPAIPDLTDSEIAKKGLNFDYLVSHNRGSQRIVPLKIFDDNQFTYMQFAHINADLPSIFTVDSEGYESLINFRISGDYVIIERVSDVFTLRYGSDTVCVFNNKYNKKKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR43</accession>
    <name>Q3YR43_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Malonyl CoA-acyl carrier protein transacylase</fullName>
            <ecNumber evidence="2 5">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0781</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the fabD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="2 5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0781"/>
    <dbReference type="EnsemblBacteria" id="AAZ68812">
        <property type="protein sequence ID" value="AAZ68812"/>
        <property type="gene ID" value="Ecaj_0781"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0781"/>
    <dbReference type="eggNOG" id="COG0331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030558_0_1_5"/>
    <dbReference type="OMA" id="AANYNCP"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024925">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004410">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc_FabD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000446">
        <property type="entry name" value="Mct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="SSF55048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00128">
        <property type="entry name" value="fabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_AT" evidence="7">
        <location>
            <begin position="7"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000446-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="326" mass="35598" checksum="D9ACE39AF3920A5C" modified="2005-09-27" version="1">MYKMILMFPGQGSQFVSMGKDIYDNFAVARYVFHEVDDILNKKLSKIMFEGSIENLTSTDNAQLALMVTSIATLRAIEYVLGKPIHLFNKVKYMAGHSVGECVALCASGALTLYEVTQLLLIRSQAMYDAVPLGYGGMCALIGGSLEDVENVVKMAQEYGVCEIANDNCDGQVVVSGVKAALEQLPDLIKDTGIKKVIKLQVSGPFHSSLMNSACDKLQQFIERIEDMKMPLVKVISNVSATEYTDCLMIKDLIAKQLISRVRWRESILYMIKQGCTCFIEVGAGTTLSGLVKRISPNVSAVSVNTASCIDSITQHFAVNAGFVNA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR44</accession>
    <name>KTHY_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thymidylate kinase</fullName>
            <ecNumber evidence="1">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmk</name>
        <name type="ordered locus">Ecaj_0780</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + dTMP = ADP + dTDP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304888.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YR44"/>
    <dbReference type="SMR" id="Q3YR44"/>
    <dbReference type="STRING" id="269484.Ecaj_0780"/>
    <dbReference type="EnsemblBacteria" id="AAZ68811">
        <property type="protein sequence ID" value="AAZ68811"/>
        <property type="gene ID" value="Ecaj_0780"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0780"/>
    <dbReference type="eggNOG" id="COG0125">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_0_0_5"/>
    <dbReference type="OMA" id="VMTREPG"/>
    <dbReference type="OrthoDB" id="1585072at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:thymidylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000023189" description="Thymidylate kinase">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <sequence length="202" mass="23141" checksum="D7252B3496367F2E" modified="2005-09-27" version="1">MFITFEGIDGSGKTTQSHLLTEYLSKIYGVNNVVLTREPGGTLLNEAVRDLLFQAKGLDSLSELLFFIAMRREHFMKVIKPSLMQKKIVVCDRFIDSTIAYQGYGQGIDCNLICQLNDLVVDMYPDITFVIDVDVHESLSRSCKNGYEFAELEFYYRVRNGFYSIVEKNPHRCHIITDNNEICDIDGINLIHLKIVKVLQMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR59</accession>
    <name>Q3YR59_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription-repair-coupling factor</fullName>
            <shortName evidence="8">TRCF</shortName>
            <ecNumber evidence="8">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mfd</name>
        <name evidence="11" type="ordered locus">Ecaj_0765</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the C-terminal section; belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the N-terminal section; belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304873.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0765"/>
    <dbReference type="EnsemblBacteria" id="AAZ68796">
        <property type="protein sequence ID" value="AAZ68796"/>
        <property type="gene ID" value="Ecaj_0765"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0765"/>
    <dbReference type="eggNOG" id="COG1197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005122_3_2_5"/>
    <dbReference type="OMA" id="SHVVHIN"/>
    <dbReference type="OrthoDB" id="234717at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000716">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00969">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036101">
        <property type="entry name" value="CarD-like/TRCF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003711">
        <property type="entry name" value="CarD-like/TRCF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004576">
        <property type="entry name" value="Mfd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037235">
        <property type="entry name" value="TRCF-like_C_D7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005118">
        <property type="entry name" value="TRCF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02559">
        <property type="entry name" value="CarD_CdnL_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03461">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01058">
        <property type="entry name" value="CarD_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00982">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141259">
        <property type="entry name" value="SSF141259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143517">
        <property type="entry name" value="SSF143517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00580">
        <property type="entry name" value="mfd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="605"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="788"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1128" mass="129839" checksum="CC3D214837576D2B" modified="2005-09-27" version="1">MGNINKFLNIPHTFEPFVIIKMYAKKLINSAIYIVDKETQLHDLASIFKFYIPSLEVITFPAWNAIPYKKNSPHNSVMAERIKVLYSLTTKCNNTPYIILSTTNAIMQKVLPQNIILQSVLKITLQQNITKHDIEYYLTHYGYNQYSIVQDTGEFAILNNSIDIFPMTYKNPIRIHLHNNFIQSINYFNHTTQLIDNNSFLDEILIYPTSELIKQKATIDLFSRIYKTKTNQDQNLLDTIISGKKHTGEENFLPLFYKEELKNIFNYIPQNSTVIFSDRIINEIEQYNSQIYLNYKSQLFYQVPPHQFYINLEDFKTITQQFNKIVFSQHNSANNNNSTSINAQSFNITTQIQHVPNFKVLAQDKQANIFEILTQYINTTCQTKKLIIACNSIGSLEYIQNKLKTLGIKLYKINHYQEIFYSHNITILPTNQSFITKSTIVITEHDLLYKQEVTNKSNISNIINNNTNLDIGDIVIHKDYGIGKIVELETTKVLDSYHDFIKIEYYNNDKLFLPVENINLISKYGQQNPNVTLDKLGSTSWQQRKTKIKNHIKKIARELLAIEAARKLSTGQIFCKDENYQHFCNEFSYTETEDQLQAIKDMEHDLSSGKIMNRLICGDVGFGKTEIALRAAFLVANKNYQVAVIVPTTLLCRQHFIVFTERFKKFPNIKIKQLSKIVERSEIKKTKESLSCGQVQIIIGTHAILAKDVTFANLSLLIIDEEQQFGVKQKESLKKIKTNVHVISLSATPIPRTLYMSLCGIKDLSLIKTSPKNRLPVTICTTHYDDTIIKDAIIREHNRGGRVFYVCPQISNIKSISDNLTKLVPGIKINTAHGQLPPTQLDTIMNDFFDGKFTILLTTSIIECGLDIPFANTIIIHNADMFGLAQLYQLKGRVGRSNIKGFVYFILSEKVTNKPTTKLEIIQSINSISSGFTLSLHDMDIRGFGNLVGEEQSGNIKDVGIELYQQMLEEELNIHNENPKDFDHINISINVNIRIPEHYIKDIGLRMRVYKKIGNLKTKQEIDNYHIELINKFGNLPNEVENLLNTIYIKQLCANIGIYEIEQVKNIIYLRIQSNTTFKQEALNYFINNPLIFKIQNNSILMLINNSSYCTTSFIIHHLGKINALNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR78</accession>
    <name>Q3YR78_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0745"/>
    <dbReference type="EnsemblBacteria" id="AAZ68777">
        <property type="protein sequence ID" value="AAZ68777"/>
        <property type="gene ID" value="Ecaj_0745"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0745"/>
    <dbReference type="HOGENOM" id="CLU_2329303_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="98" mass="11194" checksum="794AB54E8DA58C94" modified="2005-09-27" version="1">MCVLSVSVWFLAFIFNIDVHFKESSSIQLLVKKKYIVVKYIALLNIANINTSSMTALTGLQCECWICGMLFISIFIIYSMAVIFFAHGKFVYFSIVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR79</accession>
    <name>Q3YR79_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0744</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304853.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0744"/>
    <dbReference type="EnsemblBacteria" id="AAZ68776">
        <property type="protein sequence ID" value="AAZ68776"/>
        <property type="gene ID" value="Ecaj_0744"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0744"/>
    <dbReference type="HOGENOM" id="CLU_1967084_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="127" mass="14542" checksum="D4EE6A90E036E5E4" modified="2005-09-27" version="1">MSNPYCIALISLVLLLLFVLLAIALYRCCTKSKQRLEKEEQLLIKQTAKQLMGEVDLLKRENSEFVKLTDELGNELSSLQVKHSMLKNEFDKLSNMYRISNRTCYARGLAIEELEGELNICALPCEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR81</accession>
    <name>Q3YR81_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0742</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0742"/>
    <dbReference type="EnsemblBacteria" id="AAZ68774">
        <property type="protein sequence ID" value="AAZ68774"/>
        <property type="gene ID" value="Ecaj_0742"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0742"/>
    <dbReference type="HOGENOM" id="CLU_2787231_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="68" mass="7680" checksum="385A59560431D7C9" modified="2005-09-27" version="1">MSLKQEYDRKKEKPVDKEKLFSEYLSSIGSVVYAEAQANLAMASAKRIAVNANRAKEELESTVNLQCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRA9</accession>
    <name>Q3YRA9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0714</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68746">
        <property type="protein sequence ID" value="AAZ68746"/>
        <property type="gene ID" value="Ecaj_0714"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0714"/>
    <dbReference type="HOGENOM" id="CLU_531835_0_0_5"/>
    <dbReference type="OMA" id="EEFANFM"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="518" mass="60682" checksum="71089EB6348D4174" modified="2005-09-27" version="1">MLYMDKEINLSKSSPNLFKSISVVEYQLMRPQYLKEIVDSLKLEKEELGKVRTLLIMYEYNVQFYRLLVLFFRVLIRRDKSLQVLQKYTEIMAVFTLIKNKEVLLNDSFVRKCINSTINKFIRFFIKRKNAPRLAAKIERKAIEDYYEVINELNKKERKNVEQNIIEDSIEQRNGELLEKYEELVSVVSRARDQDFDVLECIPSMSEILSASDVIDNDKICNVVVSTFAISRVDMLMIKYIVFGNNGNLLKSSFLFNQIKHSFKTICSEIAYHVFGTEACQDAKTDTRKVQLYQEISVTFKNVIKIIMSSENICSAYTKIYQILSLRFLTVGKEIEFFLIKILMSYKKQDIEVYEKRVVKFLYDDQFKAMYAIIKKEYNFNDIVYANKGIKAIIKNLCDQRSNIIEQMCNMKKLLFTAENYNDILVDFLLSDSVKCDEEIVSSVTWLKHMKKNIKSPSKLNSSELLILRQDVQYVDSEIQMLNENLEVLVSRICNNSISRIRCNSVSSYSDILSKIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRC7</accession>
    <name>Q3YRC7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Colicin V production protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0696</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304805.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0696"/>
    <dbReference type="EnsemblBacteria" id="AAZ68728">
        <property type="protein sequence ID" value="AAZ68728"/>
        <property type="gene ID" value="Ecaj_0696"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0696"/>
    <dbReference type="eggNOG" id="COG1286">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108005_0_0_5"/>
    <dbReference type="OMA" id="DILPNWI"/>
    <dbReference type="OrthoDB" id="1888607at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009403">
        <property type="term" value="P:toxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003825">
        <property type="entry name" value="Colicin-V_CvpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02674">
        <property type="entry name" value="Colicin_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="200" mass="22850" checksum="C9CBF0F59F8C72DE" modified="2005-09-27" version="1">MLDVAVIGIISLSVIIGVFRGFIKEIFGLCGICISVLLTIKYHGYFSNLYSQYVVSDIISQILSTITVFILIIVAMMIVNGWIMHLLSSVRCTIIDRFGGLLIGFVKGIVFSYFLFFIIETSCYALSAPKDGDEEEKVLPSWFVNSYYYNIFYVFNTYVDDIIPETTHEKIHEVESVVQDIIEKKHTADSIKKKKFKHKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRD1</accession>
    <name>GLYA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="1">SHMT</shortName>
            <shortName evidence="1">Serine methylase</shortName>
            <ecNumber evidence="1">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glyA</name>
        <name type="ordered locus">Ecaj_0692</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304801.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRD1"/>
    <dbReference type="SMR" id="Q3YRD1"/>
    <dbReference type="STRING" id="269484.Ecaj_0692"/>
    <dbReference type="EnsemblBacteria" id="AAZ68724">
        <property type="protein sequence ID" value="AAZ68724"/>
        <property type="gene ID" value="Ecaj_0692"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0692"/>
    <dbReference type="eggNOG" id="COG0112">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_5"/>
    <dbReference type="OMA" id="VTNRNAI"/>
    <dbReference type="OrthoDB" id="861782at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="PTHR11680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000234976" description="Serine hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="127"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="1">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <sequence length="421" mass="46173" checksum="A9340D73921118EB" modified="2005-09-27" version="1">MVRYILDHNLQDIDVEVFDCISGELNRQNSQLQLIASENFVSKAVLEAQGSIFTNKYAEGYPGKRYYCGCHFADIVENIAIERLCKLFGCKFANVQPHSGSQANQGVFAALLKPGDTVVGLSLDCGGHLTHGSAPSISGKWFNAVQYQVDRNTGLLDMDEIEKLVLEHKPTLLIAGSSAYPRTIDFKRFREIADKVGAYLLADIAHYAGLIAAGEFPSPFEYAHVVTSTTHKTLRGPRGAVIMTNYEDIHKKIQSSIFPGMQGGPLMHVIAAKAVAFGEALKPDFKDYAKQIIKNSRVLVEVFKERGLNIVTDGTDSHIVLVDLRPKGVTGKDAVLALERLGIICNKNAIPFDTEKPFVTSGLRFGSAAETSRGLQESEFREIGNMVCDVIDNLKASDIVKASVEQDVIKKVKELTFAFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRE6</accession>
    <name>Q3YRE6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Multisubunit sodium/proton antiporter, MrpD subunit</fullName>
            <ecNumber evidence="4">1.6.5.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0677</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.6.5.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304786.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0677"/>
    <dbReference type="EnsemblBacteria" id="AAZ68709">
        <property type="protein sequence ID" value="AAZ68709"/>
        <property type="gene ID" value="Ecaj_0677"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0677"/>
    <dbReference type="eggNOG" id="COG1009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_9_3_5"/>
    <dbReference type="OMA" id="KRMPLTM"/>
    <dbReference type="OrthoDB" id="1887859at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="423"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="460"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="3">
        <location>
            <begin position="140"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="496" mass="55797" checksum="FE037590FDBFA4AF" modified="2005-09-27" version="1">MHFFNNLTSEFTLVCTILLPFISAIMILMLKKIKYSQEIISIISSCILFYMAFETYKFYTKHDSELYIELYEFINPKIYIAFKAEILSMIFSLLVSFLWIITTIYSISYMKNNDNNNKKQPLFYSCFAISIGCTMGIAFSANLLTLFIFYELLTISTYPLITYYINDQSKISGRYYIGILLGTSMLLFLPAIIITYNISGTLDFTTNGILPDNISNTLLITLLLLFTYGIGKAALMPIHSWLPQAMVAPTPVSALLHAVAVVKSGVFTIIKILLYIIGLKNLNAFIQEHHNILMYISGATIILASLIAIKQTNLKKLLAYSTISQLSYIVIAASLCTEYAIKVSMFQMISHAFAKITLFFTAGAIYTKTGKKYLHELHGIGMSMPITMIAFSIGAIAMIGIPPAPTFYGKFFIISEALQQKNFLVISILILSTVLNTMYFIPIIYNAFSTSYSFKYAEAPLPMLIAIITTAICTLLLFVIPYIISAFIDNYFQSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRF0</accession>
    <name>Q3YRF0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily hydrolase, subfamily IA,variant1</fullName>
            <ecNumber evidence="1">3.1.3.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0673</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0673"/>
    <dbReference type="EnsemblBacteria" id="AAZ68705">
        <property type="protein sequence ID" value="AAZ68705"/>
        <property type="gene ID" value="Ecaj_0673"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0673"/>
    <dbReference type="eggNOG" id="COG0546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_19_3_5"/>
    <dbReference type="OMA" id="WDNTLVD"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="211" mass="23443" checksum="1608E78AA835DB25" modified="2005-09-27" version="1">MMKQPTAVIFDWYNTLIDTSINTDCVILNKVLDQMGYKNIDLHSIPNSTIPKYLTTLLGKRWKEATTLYENSLEKSQKSDNFMLNDGAIELLDTLKANNIIMAIVSNKNGKSLRAEVQNKNLTHYFDSIIGSGDTGTTKPSPEPVLAALTNINVQPSKEVFFIGDSISDIQSATEAGCLPIKYGSTTTINNVLSFSNFDDIRHFICKLINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRG2</accession>
    <name>Q3YRG2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptidase M48, Ste24p</fullName>
            <ecNumber evidence="8">3.4.24.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0661</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304770.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0661"/>
    <dbReference type="EnsemblBacteria" id="AAZ68693">
        <property type="protein sequence ID" value="AAZ68693"/>
        <property type="gene ID" value="Ecaj_0661"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0661"/>
    <dbReference type="eggNOG" id="COG4783">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030556_2_0_5"/>
    <dbReference type="OMA" id="HLSQRHF"/>
    <dbReference type="OrthoDB" id="1445800at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M48" evidence="7">
        <location>
            <begin position="30"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="435" mass="50258" checksum="547D5F2E3F812F0D" modified="2005-09-27" version="1">MNIKNLILFLIVLLHSNISFCQNDYMIFRDSEVESVIKKIAFPIFTAAKINPETVRVFIVNDKMVNAYVDGNNNDVFLNYGLFKFSEDPSVLIGVLAHEVGHISQKHVLFRKNKIHNSMILSGIGYVLGIITAITVNPDMGQAIALATNDISKRMFFLYSRLQEASADQCALRYLDEAGYSNDGLVKMFKYFYSLEAQYRGNIDQYLLSHPLSYDRLLQIQNYRNHNEVHGFSDEDMYKFKRVVEKINAFFNPVERLVNDKSDINQLSPYAQSIIFYKQSDVSKALEKLDSLISKSPEDPYLYELKAQMLYKVGDIKKSVENYKLALKFSFDNVLIKLETSQALLLYDQKEAVNYLEQVTYQEPDNIFAWKQLAIAYGKTGNLGMSYFSLASKSFFENNKKDFDKYFRLAKKYLPKDSVQLERMYDLKTSLLSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRG3</accession>
    <name>Q3YRG3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304769.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68692">
        <property type="protein sequence ID" value="AAZ68692"/>
        <property type="gene ID" value="Ecaj_0660"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0660"/>
    <dbReference type="HOGENOM" id="CLU_1583156_0_0_5"/>
    <dbReference type="OMA" id="NDCYRQC"/>
    <dbReference type="OrthoDB" id="1596093at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="197" mass="22367" checksum="3E1845D2B9BB4A59" modified="2005-09-27" version="1">MKIQNTIFILLTLQAACSPIYDNKVTYKDPESESGKACVVKCAYIRNDCYRQCQVNATNCQLTEKMINVQERAKPFISIVNNNESKDKGNDTEKNSCESENLKCKKSCSNSTACETRCDMSMDMCNTQRNLNKPENFDRFFSNMSRKSAKIIEPQSISLCKVDECDRLCRSDYNLCFSSCGGQIITYKQCVAFCGKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRH4</accession>
    <name>CH60_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperonin GroEL</fullName>
            <ecNumber evidence="1">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">60 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Chaperonin-60</fullName>
            <shortName evidence="1">Cpn60</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">groEL</name>
        <name evidence="1" type="synonym">groL</name>
        <name type="ordered locus">Ecaj_0647</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide.</text>
            <dbReference type="EC" id="5.6.1.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304758.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRH4"/>
    <dbReference type="SMR" id="Q3YRH4"/>
    <dbReference type="STRING" id="269484.Ecaj_0647"/>
    <dbReference type="PRIDE" id="Q3YRH4"/>
    <dbReference type="EnsemblBacteria" id="AAZ68681">
        <property type="protein sequence ID" value="AAZ68681"/>
        <property type="gene ID" value="Ecaj_0647"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0647"/>
    <dbReference type="eggNOG" id="COG0459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016503_3_0_5"/>
    <dbReference type="OMA" id="TDTDKME"/>
    <dbReference type="OrthoDB" id="265347at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000256907" description="Chaperonin GroEL">
        <location>
            <begin position="1"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00600"/>
        </source>
    </evidence>
    <sequence length="552" mass="58659" checksum="1FA4E21BF0EC530B" modified="2005-09-27" version="1">MANVVVTGEQLDKAIREVVHILEDAVGCTAGPKGLTVAISKPYGAPEITKDGYKVIKSIKPEDPLALAIANIIAQSASQCNDKVGDGTTTCSILTAKVIEEVSKAKAAGADIVCIKDGVLKAKEAVLDALMSMKREVLSEEEIAQVATISANGDKNIGVKIAQCVQEVGKDGVITVEESKGFKELDVEKTDGMQFDRGYLSPYFVTNSEKMLVEFENPYILLTEKKLNIIQPILPILENVARSGRPLLIIAEDVEGEALSTLVLNKLRGGLHVAAVKAPGFGDRRKDMLGDIAILTGAKHVISDDLAIKMEDLTLAELGTAKNIRITKDTTTIIGSVDNSSDNVQSRINQIKVQIESSTSDYDKEKLRERLAKLSGGVAVLKVGGSSEVEVKERKDRVEDALHATRAAVEEGVVPGGGAALLYTLSVLENLKSKNDDEQLGINIIKRALQAPIKRIIRNSGSENAPCVIAHLLKQNDKELIFNVDTMNFANAFTSGVIDPLKVVRIAFDFAVSLAAVFMTLNAIVVDVPSKDDAGAAGGAGGMGGMGGMGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRI0</accession>
    <name>Q3YRI0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">5'-phosphoribosylglycinamide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">GAR transformylase</fullName>
            <shortName evidence="2">GART</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">purN</name>
        <name evidence="4" type="ordered locus">Ecaj_0641</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the transfer of a formyl group from 10-formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(6S)-10-formyltetrahydrofolate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide</text>
            <dbReference type="Rhea" id="RHEA:15053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="EC" id="2.1.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GART family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304752.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0641"/>
    <dbReference type="EnsemblBacteria" id="AAZ68675">
        <property type="protein sequence ID" value="AAZ68675"/>
        <property type="gene ID" value="Ecaj_0641"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0641"/>
    <dbReference type="eggNOG" id="COG0299">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038395_1_1_5"/>
    <dbReference type="OMA" id="TGITIHY"/>
    <dbReference type="OrthoDB" id="1815747at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08645">
        <property type="entry name" value="FMT_core_GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="3">
        <location>
            <begin position="5"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="5'-phosphoribosylglycinamide binding" evidence="2">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>(6S)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>(6S)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
        </ligand>
    </feature>
    <feature type="site" description="Raises pKa of active site His" evidence="2">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="208" mass="22882" checksum="FA0F3B9BDD5DAD04" modified="2005-09-27" version="1">MVPLRLGILISGRGSNMHALINACMQDDFPAEISCVISNNPNANGLLIAQRNNIKTFVIQGRPLDFDAIDNILKEHKVDLICLAGFMSIVPEKFINKWFHKVINIHPSLLPSFKGLSAQAQALKAGVKIAGCTVHYVYPELDAGPIIIQAAVPVFSSDTVTDLSNRILQMEHICYPKAVKLIALNQVQLDENLTTSDESLQLFYSEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRK4</accession>
    <name>Q3YRK4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SSU ribosomal protein S1P</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304728.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0617"/>
    <dbReference type="EnsemblBacteria" id="AAZ68651">
        <property type="protein sequence ID" value="AAZ68651"/>
        <property type="gene ID" value="Ecaj_0617"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0617"/>
    <dbReference type="eggNOG" id="COG0539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1098">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015805_2_1_5"/>
    <dbReference type="OMA" id="SLNEFRY"/>
    <dbReference type="OrthoDB" id="1235756at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035104">
        <property type="entry name" value="Ribosomal_protein_S1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="PTHR10724:SF7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00681">
        <property type="entry name" value="RIBOSOMALS1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="60"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="144"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="231"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="322"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="409"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="501"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="567" mass="63479" checksum="8CA42632692560EF" modified="2005-09-27" version="1">MENFQIKTNLGSEDYLQNIKKIKSNKFISHNLEAEDSEDDNSSEFQNALKEFIDDSVKEGQIIEGTIVCIDKGYVTIDSGLKSESIVSLKEFELGDDYQNIGIGSKVKLYLEKIEGRNGSVVLSREKAIRDELWQKLEEAAERKEDVEGVIFSSIKCGYTVDIKGVVAFLPASHVALRQVKDITPLLGKKQKFRILKMDNKQGNIVVSRKAILEESLADAKSLYLSQLNEGDIIEGKVKSITKYGVFIEIHESPSVGVVDGLLHITDISWSRVSHPSAVFSCGQTVKTKIIKIDRENKKISLGVKQLEESPWSNIEKKYPVDSIHKGIVTSIEDYGVFVELETGIEGLVHVSEVSWTKNSLPINQLFMRGEEIQVKVLSIDTAKSRMSLSMKRCQENPWQAFTLKYPLGSVVSTKIKNITNLGIFVSFEDNTLNDGIEGLIRTTELSWSLSPEAAVKKYSIGDSVEAKILMINPNKSKIDLGVKQLEYDPFLDLLKKTNLGDKIPVTVTKVVEDTGILVDVFNGSNSFNLLIEQEYLPDNKKFFPGDKLEAEVLLIETYNMVLSLKH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRM1</accession>
    <name>RL5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplE</name>
        <name type="ordered locus">Ecaj_0600</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304712.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRM1"/>
    <dbReference type="SMR" id="Q3YRM1"/>
    <dbReference type="STRING" id="269484.Ecaj_0600"/>
    <dbReference type="EnsemblBacteria" id="AAZ68634">
        <property type="protein sequence ID" value="AAZ68634"/>
        <property type="gene ID" value="Ecaj_0600"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0600"/>
    <dbReference type="eggNOG" id="COG0094">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_2_1_5"/>
    <dbReference type="OMA" id="ERMYAFL"/>
    <dbReference type="OrthoDB" id="1456375at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_B">
        <property type="entry name" value="Ribosomal_L5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020930">
        <property type="entry name" value="Ribosomal_L5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_L5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000242996" description="50S ribosomal protein L5">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <sequence length="177" mass="19774" checksum="DFC94AE14D2A7957" modified="2005-09-27" version="1">MLKDLYKSHIVPSLKTKLGYSNVMQVPKIVKVCLNIGLGIRGSDSKVMNSCVRDLALIAGQKPVATYAKKSIAGFKIRKGFPIGCKVTLRNNKMYEFLERLIHVVLPREQDFKGLSVNQFDGCGNLSIGIKEHISFLEIDYDKIDKILGLDINIVTNAVNNNDAKLLLMEFGFPFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRN6</accession>
    <name>Q3YRN6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sodium:alanine symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0585</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304697.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0585"/>
    <dbReference type="EnsemblBacteria" id="AAZ68619">
        <property type="protein sequence ID" value="AAZ68619"/>
        <property type="gene ID" value="Ecaj_0585"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0585"/>
    <dbReference type="eggNOG" id="COG1115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024867_1_2_5"/>
    <dbReference type="OMA" id="VSMTQVF"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015655">
        <property type="term" value="F:alanine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001463">
        <property type="entry name" value="Na/Ala_symport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30330">
        <property type="entry name" value="PTHR30330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01235">
        <property type="entry name" value="Na_Ala_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00175">
        <property type="entry name" value="NAALASMPORT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="436" mass="48079" checksum="2FCC577FAA6A9C62" modified="2005-09-27" version="1">MDLLNLVLSFPAVFLLLFTGIYLSIKSRFLQITKLPSAISLITMKKYRDKSFSIAALCTIIGGNLGVGNISGTAVALKSGGPGFALWMVIIVTLCAIIKYTTCYLSIETRVKFSKQYIGGPAIYFKNAFNTNKAVIVFTILMLICSIAIGNFVQVNSLSIPMQLINKPPVIAGLFMCFMFFAVTTLRLEIISALISRLVPAMAVAYIILSSFVLYKFSDNIIPSIKLMFSNFLTFESFKSGMIGAFILETFHIIQVGTFRGIFATDIGLGLEGMVHSSINSTSKNFNTNQSLISLISPFIVVIVTLVTTLVILVTNTWLGPLESTNMCIAAFKSAFDGQYINYIIIGIMFCFSFTTIFTWFVCAKHTLYCLTGGKDSLCIKMWKVLYTIIIPIGALGKVQFLWDIADISISLILISNTIAILILLPKYKDIFKCSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRN9</accession>
    <name>Q3YRN9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4-hydroxybenzoate octaprenyltransferase</fullName>
            <ecNumber evidence="8">2.5.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">4-HB polyprenyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ubiA</name>
        <name evidence="9" type="ordered locus">Ecaj_0582</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of ubiquinone-8 (UQ-8) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate 3-octaprenyl-4-hydroxybenzoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>4-hydroxybenzoate + all-trans-octaprenyl diphosphate = 4-hydroxy-3-all-trans-octaprenylbenzoate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27782"/>
            <dbReference type="ChEBI" id="CHEBI:1617"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57711"/>
            <dbReference type="EC" id="2.5.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304694.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0582"/>
    <dbReference type="EnsemblBacteria" id="AAZ68616">
        <property type="protein sequence ID" value="AAZ68616"/>
        <property type="gene ID" value="Ecaj_0582"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0582"/>
    <dbReference type="eggNOG" id="COG0382">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034879_1_1_5"/>
    <dbReference type="OMA" id="MVVYPYG"/>
    <dbReference type="OrthoDB" id="1472516at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002083">
        <property type="term" value="F:4-hydroxybenzoate decaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047293">
        <property type="term" value="F:4-hydroxybenzoate nonaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008412">
        <property type="term" value="F:4-hydroxybenzoate octaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01635">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006370">
        <property type="entry name" value="HB_polyprenyltransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PTHR11048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="295" mass="33170" checksum="7776BB327D1639B8" modified="2005-09-27" version="1">MLCKLKDIVPNFDKYIELLGLHMVETFFLALFPGLVSVVLVSHDVLSACWYMLLCIVGTSIVKPAGCVINCICDREIDAKVKRTKNRPLADKSLTVVQALKVLVVLLLCSCGLLVFTNMYTVKLSIITMILIVIYPLSKRFFAWPQFLLGLVFNSGVLLVCSMITGRLTLSCILLYIGCIFWTIGYDTIYAAQDRECDAKLGLNSTALRFGNDIRLWIGRVYTITVAMWMSAGVVAMLHPVFYIAVLIIAVIFYYQYKKSDFDNSEKCMYMFNMNAYIGLVLFVGAVFGRVLNVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRP6</accession>
    <name>PLSX_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate acyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.274</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acyl-ACP phosphotransacylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Acyl-[acyl-carrier-protein]--phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-acyl-ACP acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">plsX</name>
        <name type="ordered locus">Ecaj_0575</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a fatty acyl-[ACP] + phosphate = an acyl phosphate + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.274"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Probably interacts with PlsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Associated with the membrane possibly through PlsY.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PlsX family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.274" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRP6"/>
    <dbReference type="SMR" id="Q3YRP6"/>
    <dbReference type="STRING" id="269484.Ecaj_0575"/>
    <dbReference type="PRIDE" id="Q3YRP6"/>
    <dbReference type="EnsemblBacteria" id="AAZ68609">
        <property type="protein sequence ID" value="AAZ68609"/>
        <property type="gene ID" value="Ecaj_0575"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0575"/>
    <dbReference type="eggNOG" id="COG0416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039379_1_0_5"/>
    <dbReference type="OMA" id="HGKSNAR"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043811">
        <property type="term" value="F:phosphate:acyl-[acyl carrier protein] acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00019">
        <property type="entry name" value="PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003664">
        <property type="entry name" value="FA_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012281">
        <property type="entry name" value="Phospholipid_synth_PlsX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30100">
        <property type="entry name" value="PTHR30100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02504">
        <property type="entry name" value="FA_synthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002465">
        <property type="entry name" value="Phsphlp_syn_PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00182">
        <property type="entry name" value="plsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000001757" description="Phosphate acyltransferase">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00019"/>
        </source>
    </evidence>
    <sequence length="337" mass="36105" checksum="07076DB216347C69" modified="2005-09-27" version="1">MSIISIAVDAMGGDFAPEAVIGGLDFALTNLLDNKDVSFNIYGQESQVLPMLDKYKNVKEHSLFIDTPDIVLANDKPSFALRKRKSSSMWCAIESIKNGVTSGVVSSGNTGALMAISCFVLGTLPNIKRPAICCALPSKCERYFVILDLGANVDCSANSLFQFAIMGNAFAKAVLNVPNPKVALLNVGEEEVKGTDVIREAFLLLRQAESSINFCGYIEPINMLGGEVDVVVADGFCGNVVLKVAESIAYIFKSVFEKSVSSSIITKIAGLLLKSSMKRNFMKFNPKIYNGAMLVGLNGVVVKSHGNADKIAFAHAVKVAVNAARNNINAKIIHELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRQ3</accession>
    <name>Q3YRQ3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Farnesyl-diphosphate synthase</fullName>
            <ecNumber evidence="3">2.5.1.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0567</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0567"/>
    <dbReference type="EnsemblBacteria" id="AAZ68602">
        <property type="protein sequence ID" value="AAZ68602"/>
        <property type="gene ID" value="Ecaj_0567"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0567"/>
    <dbReference type="eggNOG" id="COG0142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_0_1_5"/>
    <dbReference type="OMA" id="VEMVHAA"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:geranyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="283" mass="31496" checksum="0F17E4E3AD2047DB" modified="2005-09-27" version="1">MKQRLEGILSCNISELMKHYSSLLIQKLESMLPENNQDILLTSIRYSIFSPGKHIRPFLVNAVAQMFDVNVDRTLPVSAAVEIIHVYSLIHDDLPGMDNEEIRRGQASSHKKFNEAVAILTGDALLTLAFEILSTINESPLIRCRIIKTLAHAIGYQGMIQGQILDTETIAKDINEIQNIHVLKTAQFFSAICELGGILGNASNIQLNALANYGLNLGHAFQIKDDIADEDQDKSKNNILNIINNKDAKNYASNLIDKSIEHLTIFSHKADILHSLAGFIRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRR0</accession>
    <name>Q3YRR0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multisubunit sodium/proton antiporter, MrpF subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0560</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPA3 antiporters (TC 2.A.63) subunit F family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0560"/>
    <dbReference type="EnsemblBacteria" id="AAZ68595">
        <property type="protein sequence ID" value="AAZ68595"/>
        <property type="gene ID" value="Ecaj_0560"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0560"/>
    <dbReference type="eggNOG" id="COG2212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125825_2_1_5"/>
    <dbReference type="OMA" id="MSVMLCC"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007208">
        <property type="entry name" value="MrpF/PhaF-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04066">
        <property type="entry name" value="MrpF_PhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="105" mass="11614" checksum="579869113F02E4D6" modified="2005-09-27" version="1">MLIFVYIDFITIIVNSMLVFTGCVLLFCMGAMLICVATGNSIYDKILAANLFGTYSVVLIVVVGVINNTFSFLIDVSLVYACINFISTIAFMRFFLHGSFGKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRR3</accession>
    <name>Q3YRR3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multisubunit sodium/proton antiporter, MrpB subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0557</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304670.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0557"/>
    <dbReference type="EnsemblBacteria" id="AAZ68592">
        <property type="protein sequence ID" value="AAZ68592"/>
        <property type="gene ID" value="Ecaj_0557"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0557"/>
    <dbReference type="eggNOG" id="COG2111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101659_3_1_5"/>
    <dbReference type="OMA" id="FHGDYGP"/>
    <dbReference type="OrthoDB" id="1962104at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="MnhB" evidence="8">
        <location>
            <begin position="6"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="139" mass="14958" checksum="39D5A101372254EF" modified="2005-09-27" version="1">MFKNPVLRTIYILMVPIIILYGLYVQFHGDYSPGGGFQAGVIVASAIILHGILFGVDMTSKAISQYIVKLICGIGILIYGGTGIASMLLGGEFLSYSVLMSDPVLGQELGIFIVELGVGMTVCSSMLIIFFNFVSSYKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRR6</accession>
    <name>DNAK_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein DnaK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">HSP70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heat shock 70 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heat shock protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaK</name>
        <name type="ordered locus">Ecaj_0554</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a chaperone.</text>
    </comment>
    <comment type="induction">
        <text evidence="1">By stress conditions e.g. heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304667.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRR6"/>
    <dbReference type="SMR" id="Q3YRR6"/>
    <dbReference type="STRING" id="269484.Ecaj_0554"/>
    <dbReference type="PRIDE" id="Q3YRR6"/>
    <dbReference type="EnsemblBacteria" id="AAZ68589">
        <property type="protein sequence ID" value="AAZ68589"/>
        <property type="gene ID" value="Ecaj_0554"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0554"/>
    <dbReference type="eggNOG" id="COG0443">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_2_1_5"/>
    <dbReference type="OMA" id="ISIKRHM"/>
    <dbReference type="OrthoDB" id="161217at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="SSF100920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="SSF100934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000225961" description="Chaperone protein DnaK">
        <location>
            <begin position="1"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="597"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="597"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="617"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine; by autocatalysis" evidence="1">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="634" mass="68963" checksum="B929AC51BF655422" modified="2005-09-27" version="1">MAVIGIDLGTTNSCVAVMEGGDAKAIENSEGARTTPSIVAFTDSERLVGDPAKRQATTNAKNTIYASKRLIGRRYQDVKDIKSSYEVVSAKNGDAWIKVLGKEYSPSQIGAFVLEKMKETAERHLGHKVEKAVITVPAYFNDAQRQATKDAGKIAGLDVIRIINEPTAAALAYGLNKSDKQKVIAVYDLGGGTFDVSILEIADGVFEVKATNGDTMLGGEDFDHAIMDYLMDDFKKTTGIDLHNDAMAVQRIKEASEKAKIELSNRMETDINLPFISSDSTGPKHLSLKLTRAKFENLVDDLIQRTIEPCKKALKDAGISADKIDEVVLVGGMTRVPKVIQKVKEFFGREPHKGVNPDEVVAIGAAIQGSILAGDVRDVLLLDVTPLSLGIETLGGVFTPLIERNTTIPTKKSQVFSTAEDGQTAVTIKVYQGERKMAADNKLLGQFSLEGIPSAPRGMPQIEVTFDIDANGIVHVSAKDKASGKEQAIKIQSSGGLSDDEIQRMIKEAEQKAGEDEKRKKFIELKNNGENLVHSTEKSLNEYGDKIPNSDRLEIENAIRDVRDALGNSDVESVDILQQKVDHLMKVSMKLGEALYGNANNTSSTESTTTNNNNEEDSKVVDSDYQEIDKKDGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRS0</accession>
    <name>ERA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTPase Era</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">era</name>
        <name type="ordered locus">Ecaj_0550</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304663.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRS0"/>
    <dbReference type="SMR" id="Q3YRS0"/>
    <dbReference type="STRING" id="269484.Ecaj_0550"/>
    <dbReference type="PRIDE" id="Q3YRS0"/>
    <dbReference type="EnsemblBacteria" id="AAZ68585">
        <property type="protein sequence ID" value="AAZ68585"/>
        <property type="gene ID" value="Ecaj_0550"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0550"/>
    <dbReference type="eggNOG" id="COG1159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038009_1_1_5"/>
    <dbReference type="OMA" id="WAEVDVI"/>
    <dbReference type="OrthoDB" id="984717at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04163">
        <property type="entry name" value="Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00367">
        <property type="entry name" value="GTPase_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030388">
        <property type="entry name" value="G_ERA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005662">
        <property type="entry name" value="GTP-bd_Era"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698">
        <property type="entry name" value="PTHR42698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00436">
        <property type="entry name" value="era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51713">
        <property type="entry name" value="G_ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000079692" description="GTPase Era">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Era-type G" evidence="2">
        <location>
            <begin position="7"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="1">
        <location>
            <begin position="205"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="2">
        <location>
            <begin position="15"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="2">
        <location>
            <begin position="41"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="2">
        <location>
            <begin position="62"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="2">
        <location>
            <begin position="124"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="2">
        <location>
            <begin position="153"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="15"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="62"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="124"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01050"/>
        </source>
    </evidence>
    <sequence length="296" mass="33719" checksum="D5632E82DB85797F" modified="2005-09-27" version="1">MDHIDKKCSMNAIVGTTNAGKSTLINMLVGRKVAAVTPKVQTTRVRMHAVLNNENVQLIFIDTPGIFSPKTKLEKFIVKHAWMSLKGIENVILLLDVKNYLNKHIEKIISRIKQSNINAILVVNKIDMVSQALVDKAIEYMYSLHNFSKTFTISALHDIGLNRLINYLCEISPSGPWLYPEGQISDAPLKFFIAEITREKLFLSLHHELPYSLSVVTEALEEKVDGSLIVKQVIYVTKDNHKTIILGKKGEMIKKISIESRSELEKILDLKIHLFLFVKVRELWQDHLNECVGYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRS2</accession>
    <name>Q3YRS2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0548"/>
    <dbReference type="MEROPS" id="A32.002"/>
    <dbReference type="EnsemblBacteria" id="AAZ68583">
        <property type="protein sequence ID" value="AAZ68583"/>
        <property type="gene ID" value="Ecaj_0548"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0548"/>
    <dbReference type="eggNOG" id="COG3577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099411_0_1_5"/>
    <dbReference type="OMA" id="VVPRGQM"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05483">
        <property type="entry name" value="retropepsin_like_bacteria"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011969">
        <property type="entry name" value="Clan_AA_Asp_peptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034122">
        <property type="entry name" value="Retropepsin-like_bacterial"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="SSF50630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02281">
        <property type="entry name" value="clan_AA_DTGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="156" mass="17596" checksum="8600D9A27D30135E" modified="2005-09-27" version="1">MMFFSSAKNDISSKYFDKFFPNSVIHNKKVTYVDGGGVEFRRAKDGHFYIEATVHGIPINFLVDTGATDVVLSVEDAKRLKKHLKYLNKKKTYHTANGTIKALYVEISEMQIGGFVVNNVKASVNVSPMRTSLLGMSFLQYFNFNMSGDKLTLNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRT8</accession>
    <name>Q3YRT8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TrbL/VirB6 plasmid conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ecaj_0531</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TrbL/VirB6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304645.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0531"/>
    <dbReference type="EnsemblBacteria" id="AAZ68567">
        <property type="protein sequence ID" value="AAZ68567"/>
        <property type="gene ID" value="Ecaj_0531"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0531"/>
    <dbReference type="eggNOG" id="COG3704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_319288_0_0_5"/>
    <dbReference type="OMA" id="KEHITWV"/>
    <dbReference type="OrthoDB" id="290497at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030255">
        <property type="term" value="P:protein secretion by the type IV secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007688">
        <property type="entry name" value="Conjugal_tfr_TrbL/VirB6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04610">
        <property type="entry name" value="TrbL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="552"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="580"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="651"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="687"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="733"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="769"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="809"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="882"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="259"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="882"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="905"/>
            <end position="919"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="935" mass="105616" checksum="8ADA29AB0780DCCD" modified="2005-09-27" version="1">MFRIVLIISVFLLSSCGYHGCIKPQNVMFDEYSSTVETGLQDGKKEHITWVKSDLILAGKKDLIIRVNTVNVNYCHNTNYVVDIYADQGDSTLSGKKFYLGIPNIMAGEKLNFRLYPAFNFTVDKDICSNKNPQIYAQNTDKCMKDYLDTKYYIAIDNYYGSQMNNLMYLKDRSSHDKAREWINFPNKILYNPDKSNTIYQYLNNQDSINGIGKVNSQNNNNGEMFYLCATGKEIDELVNKTIDEEIKKKQQQGNPSTIKVSEHEKKELVEKYKEAISKKQDEVRNKYKKQYDAYSVNMICGNLCNIPDYENKKVEKDCFVVEMNNIFNACTVTADGNLSGCDFENVMQYNTYLPFLYLKHNSGITNFITIDDTIRKWQSDNNRSSDIGLLTNYSYLISKNILDKGLYLEVNVDKNLELTGKYKIEVDKDCTNHVKDSLYYVISKGPPKVKPGDSGTTKIDFIGNEDTKVKLSDKDAAGDLYFGIKDNGDGYENNTGYFNITVISKKRIPNVISYIVEHLKDALERGLYGTSSTNSGGVNLIYNSIVKNLHFIQIVNSLLVLYVLINALFYFVGFSKASIFELLVITLKIGVIIYVIGPNSWEFFNNHLFKLFTEAPIQLIAIMTGQDPAETTSFEFLDLVLYRFSLSETWIQILALLFTGPVGWVSVALIFWGLIVLFLTVATAVITYLISIILIGLLLSIAPFFIICILFKRTKAIFDAWIKSLVQTAMQPVIIFASFALLTEVIDNIIYSMFAFESCDTCVLKPEFDLKIMKLSFCILEFLLPVGISPLSTFNDNLKDSVNGDGSVMFLGLPVPFMKVLIFVIMVNATKHFIGSAGEMCTSIFGSFANLSAVAENAKESALGIIGMDQNSRMQRARYEEMNKTSDRDNDGERKRDPLGLQSPITENDEQPISRRQSRSGVEIPEGPPSSFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRW9</accession>
    <name>Q3YRW9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304614.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68536">
        <property type="protein sequence ID" value="AAZ68536"/>
        <property type="gene ID" value="Ecaj_0500"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0500"/>
    <dbReference type="HOGENOM" id="CLU_1459154_0_0_5"/>
    <dbReference type="OMA" id="PHLYDPW"/>
    <dbReference type="OrthoDB" id="1797236at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="185" mass="19612" checksum="023B619A73BF7A00" modified="2005-09-27" version="1">MEGNEGGGINVSALQSYLHQQQSAGTSQEDEETVFSVFVFLTWFYKNVPCYLDSVTGNISASVTCLGMSLPTTIVLGSEMQKNIFGLPKLLPDPQPGDATAGDAGGGADDGAAAGGDDPDAMQLVHPDDPYAQDNYPHADQDVAAAAGHGLDDVALQQEPEYHHDRSPSPTPVTQHGNQYDHDFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRX2</accession>
    <name>Q3YRX2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68533">
        <property type="protein sequence ID" value="AAZ68533"/>
        <property type="gene ID" value="Ecaj_0496"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0496"/>
    <dbReference type="HOGENOM" id="CLU_2329302_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="98" mass="11000" checksum="F2D39E0D5E01ECE8" modified="2005-09-27" version="1">MELILLFEGNVPILYPAHVCTYDKSQDAYRIDYPAIDFTTNVTRPGDDVPLNELYGSMFNKYCIISNTSSPKIIYCLYGTTRGGGGGEHSIYNSCFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRX5</accession>
    <name>Q3YRX5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Ribosomal RNA small subunit methyltransferase E</fullName>
            <ecNumber evidence="4 11">2.1.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0493</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) + N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42920"/>
            <dbReference type="Rhea" id="RHEA-COMP:10283"/>
            <dbReference type="Rhea" id="RHEA-COMP:10284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74502"/>
            <dbReference type="EC" id="2.1.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the RNA methyltransferase RsmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.193" evidence="4 11"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304608.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0493"/>
    <dbReference type="EnsemblBacteria" id="AAZ68530">
        <property type="protein sequence ID" value="AAZ68530"/>
        <property type="gene ID" value="Ecaj_0493"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0493"/>
    <dbReference type="eggNOG" id="COG1385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067442_4_0_5"/>
    <dbReference type="OMA" id="IGPEGCF"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006700">
        <property type="entry name" value="rRNA_ssu_MeTrfase-E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30027">
        <property type="entry name" value="PTHR30027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04452">
        <property type="entry name" value="Methyltrans_RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015601">
        <property type="entry name" value="MTase_slr0722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00046">
        <property type="entry name" value="TIGR00046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="9 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="239" mass="26649" checksum="E56746F2447C3EAE" modified="2005-09-27" version="1">MQKRSLNVRLYVTCALNKSSIITLDKLASHYICNVMRAANLDEIKLFNGKDGEWIGEIFEVSHNTKIIVNEQIRKQVATKCLVLCFAIVKNTALQNVVRQSTEMGVTLMQPLYTKHIATSNINLDKCKKWAIEAAEQCERLDIPDILSPISFVDLGKLKSSDNNFVICDETGKGKHPSEVLKNKDNIHIIIGPEGGFSNDELNFAYSFCDGLSLGTRILKVDTAVVSALAYVNEYYSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRY3</accession>
    <name>Q3YRY3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304600.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68522">
        <property type="protein sequence ID" value="AAZ68522"/>
        <property type="gene ID" value="Ecaj_0485"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0485"/>
    <dbReference type="HOGENOM" id="CLU_118039_0_0_5"/>
    <dbReference type="OrthoDB" id="2125385at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="200" mass="23344" checksum="C744A57DCF4E79FC" modified="2005-09-27" version="1">MNLILLVLRDNRPVLLPTAKCKYHKELERYSVPGDVVNDRLGIPNAENFENPYCEVHILYGVVFKKYNLVTSVSLQENIMFLFGENPVSGCSAFFVFRLQKDLLGSVLQHGLELDNHMILGSGIIEKRDISYEKYTKDLFELIKTRLSIISFCKQSIRTHMLSFFNEYGELFYTVYQNTVVYDTKTDVITNERYDIIRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRZ2</accession>
    <name>Q3YRZ2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH-quinone oxidoreductase subunit L</fullName>
            <ecNumber evidence="3">7.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NADH dehydrogenase I subunit L</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">NDH-1 subunit L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Ecaj_0476</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304591.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0476"/>
    <dbReference type="EnsemblBacteria" id="AAZ68513">
        <property type="protein sequence ID" value="AAZ68513"/>
        <property type="gene ID" value="Ecaj_0476"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0476"/>
    <dbReference type="eggNOG" id="COG1009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_6_0_5"/>
    <dbReference type="OMA" id="LIGFWQH"/>
    <dbReference type="OrthoDB" id="1274678at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010934">
        <property type="entry name" value="NADH_DH_su5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018393">
        <property type="entry name" value="NADHpl_OxRdtase_5_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="PTHR42829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06455">
        <property type="entry name" value="NADH5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01974">
        <property type="entry name" value="NDH_I_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="309"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="409"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="454"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="595"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_N" evidence="15">
        <location>
            <begin position="61"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="14">
        <location>
            <begin position="127"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="NADH5_C" evidence="16">
        <location>
            <begin position="427"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF06455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAZ68513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="621" mass="70383" checksum="AD9F87DBA857CE05" modified="2005-09-27" version="1">MIKIELLCVFLPLIGSIFGKMINNRFASQLVITCLVICASLLSWYLFFNFKDSYVITLFPWIELYKLKINWSLCIDSLSIIMLIIVNTVSAIVHLYSISYMSHDAGASRFLSYVSLFTFFMLILITSDNFVQLFFGWEGVGLCSYLLIGFWFEKASATKAAMKAFIMNRIGDFFFILGIIAVFWVFGSLEFSKIFGMLDSVYVEGKMNFLGYPMLYVDVICLLLFIGCMGKSAQIGLHTWLPDAMEGPTPASALIHAATMVTAGVFLVARCSPLFEVSELARNVILIVGTCTCFFAATVAIVQDDVKKIIAYSTCSQLGYMFIACGLSVYSIAIFHLMTHAFFKALLFLCAGNIIHAAHEQNIHKINVSWRQIPFTYVLTWIGSLALAGIFPFAGFYSKDLIIESSYHVNTISFIVCNFVAFLTSFYSWRLIILVFHGVNLKRESIHESGKVMLLPLLILAIGSVFAGMWGQNLLLINSPAFWNSSIIVHEHEEVGLFVKLLPLILSVFGIVCAYLRYFYNYFDNWRSNKLLKFLYNKWYFDELYNIVFVVPIKCIANLFSKVIDKKIIDYFGLGGITRLVDSCSKGSIKIQTGFIFDYAFVMLLGLISIIMWLIYNNIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRZ3</accession>
    <name>Q3YRZ3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH-quinone oxidoreductase subunit M</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">NADH dehydrogenase I subunit M</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">NDH-1 subunit M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0475</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304590.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0475"/>
    <dbReference type="EnsemblBacteria" id="AAZ68512">
        <property type="protein sequence ID" value="AAZ68512"/>
        <property type="gene ID" value="Ecaj_0475"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0475"/>
    <dbReference type="eggNOG" id="COG1008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_4_4_5"/>
    <dbReference type="OMA" id="ITRWGNQ"/>
    <dbReference type="OrthoDB" id="535707at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010227">
        <property type="entry name" value="NADH_Q_OxRdtase_chainM/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507">
        <property type="entry name" value="PTHR43507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01972">
        <property type="entry name" value="NDH_I_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="8">
        <location>
            <begin position="122"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="485" mass="54039" checksum="59C22EA62360E5B5" modified="2005-09-27" version="1">MLFFMVLLPILGSCLLAVSNFKSDSLFIKLVSLLCSGAAFIINIIIAVKFDYLCNGFQFVYRLTQGFAVGIDGLSLPLLLLTTFLFLLSVVFALYNMKSSNLRVLLALLLLLEGLTIGVFASLDIIMFYIFFEAVLIPMFFIIGIWGHEDRVYATFKLFLYTLVGSLLFLIALLYVYFFSDRVSDIEKLTYILSNYLNLTAQKWVWIAFFVSFAIKIPVVPFHTWLPDAHVQAPTVGSVLLAGILIKIGTYALLRFSIPMLPEASVYFANFVIVLSIIAVIYASLISFVQNNIKKLIAYSSIAHMGFVTAGIFSFNEYGISGAVFQMISHGLISAALFTCVGILYNKTHTLEISKYSGLAKIMPQFSIMFIFFSMASIALPGTSGFIGEFLSILGVFHYSKLFSVLIAVGVILGALYMLFLCKKIIWGAAPNCDLIIDCNLSKSELFILATLAAFILLFGLYPYYILLKCLTPLVEQLYLKNFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRZ9</accession>
    <name>Q3YRZ9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acriflavin resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304584.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0469"/>
    <dbReference type="EnsemblBacteria" id="AAZ68506">
        <property type="protein sequence ID" value="AAZ68506"/>
        <property type="gene ID" value="Ecaj_0469"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0469"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_5"/>
    <dbReference type="OMA" id="GDNVIFW"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="460"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="532"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="856"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="880"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="914"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="959"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1038" mass="116628" checksum="BB404BBB3D06785B" modified="2005-09-27" version="1">MRKIAEIFLRRNRVSIFLLMMILVFGCYSYFNIPKEKNPEIKKPILSIYTTLQGMSSEDVEKLLVYPIEREMKAIKGISRISSVAKTGYANVILEFNTGFDYKEALENVRSKIDIIRAKLPQEATFPMISEHNFSLFPVLSIALIGDLPDRTLFKIARNLQSKLESLPSIESVKIIGNSKDIVEVNISPDVINSYNLQMQDVVMAILNNNQFVNAGNLDSVIGKYTLHLPGLLKNVQDIMNIPIKTDGYSVVTIGDVANIKLVYQDNKEFVRVNHHPCLVLEISKQAGKNIVETIFQVKGLISNLSDVLPKNLSIVYLQDESEEILDILNELQNAILISVLLVVIIMMIFMGTKTALLVAISIPGSFLFGMIIMYFLGYTLNIVILFSLIMAVGMLVDDAIVVTEYADRKMIDGLNKTEAFKQAAYDMFWPVASATLTKLVVYIPLLFWPGTTGEFMKYIPIALITTLSGSWIMALIFIPVLGSIIGTPSVTDKKNIAKIVAISTGDFIKLGSFMRGYSQLLKYILDRPKKFICIVVSILFSSTLGYFIVGPGIEFFPDIESERMLITIRSSNNLSVNERDNILKEIEKRICNVSGIKFIYAKAGLFEYSLDVDDIIGKIQVELEKWSVRRKSEKILTEIKSKLQDIKGIIINIAEDKMKPVIGKPIRIDVSSYNVAKVNDVTNKIMSIMNNSLGFENVTDNRPSLEIEWKVDADKHKAIKSGVDISMINRFIKMIAGGVLLNKYHPDNVDDEVDIVVKFPKEYRSLNTLNNLFVSSFNGYTAVSNFVVYRPQYKEGRINHVDGMRTVSITSDLSQGYLLEKMVSFLKQKFKEDIDPEVLVEIKGEIKDQEESQGFLIKAFFVIVLLIMLILITEFNSIYDALIIMTAIFLSTTCVFFGFFITGHVFSVVMGGVGIIVLAGVIVNNNILLLDAFYINMKSICDKKDAIIMAALSRVRPILLTVVTGIIGLVPMILRINFDFINRQIICNSPSSQLWFELSYTIAIGLFLATIITLFFTPALLMIGKYNKINKSIIKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-04-07" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS02</accession>
    <name>Q3YS02_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome-binding factor A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rbfA</name>
        <name evidence="3" type="ordered locus">Ecaj_0466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer. Binds 30S ribosomal subunits, but not 50S ribosomal subunits or 70S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RbfA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304581.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0466"/>
    <dbReference type="EnsemblBacteria" id="AAZ68503">
        <property type="protein sequence ID" value="AAZ68503"/>
        <property type="gene ID" value="Ecaj_0466"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0466"/>
    <dbReference type="eggNOG" id="COG0858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089475_1_1_5"/>
    <dbReference type="OMA" id="FIRKAIF"/>
    <dbReference type="OrthoDB" id="1971380at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00003">
        <property type="entry name" value="RbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000238">
        <property type="entry name" value="RbfA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023799">
        <property type="entry name" value="RbfA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020053">
        <property type="entry name" value="Ribosome-bd_factorA_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33515">
        <property type="entry name" value="PTHR33515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02033">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89919">
        <property type="entry name" value="SSF89919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00082">
        <property type="entry name" value="rbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01319">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0690">Ribosome biogenesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="115" mass="13486" checksum="0A012F072BEB8F78" modified="2005-09-27" version="1">MIYKSENFRNLKVASVLNRAISKVLMQDICLSNNHLITISKVEVSNDIRNANVFIIISQDCTDKEAVIKELNDSSYFIRKAIFPYVDLRYIPRLYFKQDYCFDNLVKINQILESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS30</accession>
    <name>Q3YS30_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68475">
        <property type="protein sequence ID" value="AAZ68475"/>
        <property type="gene ID" value="Ecaj_0433"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0433"/>
    <dbReference type="HOGENOM" id="CLU_1978031_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="126" mass="14886" checksum="E1932B132493E7FD" modified="2005-09-27" version="1">MNILINNKFSEINIIQNTLVSYNYAVMYSLYKNNKLALAIQIDKDITIDDMLNDFKFLSKLIVYVGYTTSHNKTFCKKVKQHKVLDDPYPNIKLMRLLTVSIFYYYIKDRILIICVKVVYMVVLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS39</accession>
    <name>Q3YS39_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304544.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68466">
        <property type="protein sequence ID" value="AAZ68466"/>
        <property type="gene ID" value="Ecaj_0423"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0423"/>
    <dbReference type="HOGENOM" id="CLU_1080664_0_0_5"/>
    <dbReference type="OrthoDB" id="2090025at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021387">
        <property type="entry name" value="DUF3023"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11224">
        <property type="entry name" value="DUF3023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="257" mass="29429" checksum="2BD6220B2F6FBED7" modified="2005-09-27" version="1">MLGYDSENKVFINEQLSEELVQTSETEGLICIRCYCVGNSSSGRLKVCVNEEQCFNNEIPVDGQSVVCCEFILPDRICDHQLVRKTIDNTLKVQYVKCYLVIAKEQSQKFFNEVYKNFLLESPMRLKVCNIRQYGNVCLSMLTNTQLYTESMKEAFGIIKFNAEFVEYSKQVIMFHSKGQKQHNKNQECGATGTTVVGMCKKAFKSFSRRVKKNVGEHDEQRLVCEKDEYDDGQDRPMVLGSKETTADRWGGGGKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS42</accession>
    <name>Q3YS42_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304541.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0420"/>
    <dbReference type="EnsemblBacteria" id="AAZ68463">
        <property type="protein sequence ID" value="AAZ68463"/>
        <property type="gene ID" value="Ecaj_0420"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0420"/>
    <dbReference type="eggNOG" id="ENOG5033NGP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_201846_0_0_5"/>
    <dbReference type="OMA" id="NPIEWAS"/>
    <dbReference type="OrthoDB" id="2005471at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="72" mass="7950" checksum="2784823B95831157" modified="2005-09-27" version="1">MAKKGAKVAKTASKNNPLQRKKGTAQKMYNGKMIKPVKYIDRELGTIFMAGQYDNGDLVQDSSGNPIEWASM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS44</accession>
    <name>PYRB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="1">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartate transcarbamylase</fullName>
            <shortName evidence="1">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrB</name>
        <name type="ordered locus">Ecaj_0418</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304539.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS44"/>
    <dbReference type="SMR" id="Q3YS44"/>
    <dbReference type="STRING" id="269484.Ecaj_0418"/>
    <dbReference type="EnsemblBacteria" id="AAZ68461">
        <property type="protein sequence ID" value="AAZ68461"/>
        <property type="gene ID" value="Ecaj_0418"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0418"/>
    <dbReference type="eggNOG" id="COG0540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_2_0_5"/>
    <dbReference type="OMA" id="FPTEREY"/>
    <dbReference type="OrthoDB" id="1844275at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF16">
        <property type="entry name" value="PTHR11405:SF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000301570" description="Aspartate carbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <sequence length="305" mass="34098" checksum="592C198557B65871" modified="2005-09-27" version="1">MNTLLEISSLTPDDIESIFNITMQYFNNRSQNNKILNGKVVVNLFFESSTRTLSSFEIAEKSLGAHSITLNINTSSINKGESIIDTILNINAMNPDLIIIRSQYSQLVKTISQYVSNCCIINAGDGNHEHPTQALIDYCTIRYLKKNISNLNISICGDILHSRVARSNIRLLSRYGANISLIAPPTLACDLVGVSHIYHNLIDGIRDADVIMLLRLQKERITNCVIPSEEEYSHLYMLNSERLSYAKKDVIIMHPGPTNKGTEISNNVAENKSVILLQVKMGVAVRKAILHYLLYGNKCNTSNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS47</accession>
    <name>Q3YS47_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304536.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68458">
        <property type="protein sequence ID" value="AAZ68458"/>
        <property type="gene ID" value="Ecaj_0415"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0415"/>
    <dbReference type="HOGENOM" id="CLU_1364415_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="200" mass="22000" checksum="888BD9C165AA72A7" modified="2005-09-27" version="1">MNNKTVDNILKAINALLFLFCVIIPIVLAICELSNLSVPSFMYKIDMFVIMVTTLAMYIVSFIDRDYVKLSYRQVDKKSKQIIFHSIGTAIIAAGILLPLGLTCYDIYSIVTGNGVNIAVGYQIAAYSMLALYAMSTVIFLVCGVDIVAQQVVSCTRQNVNDSECESLIASDANGYDEVNCNVESVIIDDCSNECVKNDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS68</accession>
    <name>Q3YS68_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative immunogenic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304515.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YS68"/>
    <dbReference type="STRING" id="269484.Ecaj_0394"/>
    <dbReference type="EnsemblBacteria" id="AAZ68437">
        <property type="protein sequence ID" value="AAZ68437"/>
        <property type="gene ID" value="Ecaj_0394"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0394"/>
    <dbReference type="eggNOG" id="COG2358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033215_0_1_5"/>
    <dbReference type="OMA" id="VAQSDWQ"/>
    <dbReference type="OrthoDB" id="991310at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011852">
        <property type="entry name" value="TRAP_TAXI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42941">
        <property type="entry name" value="PTHR42941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16868">
        <property type="entry name" value="NMT1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02122">
        <property type="entry name" value="TRAP_TAXI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230979" evidence="1">
        <location>
            <begin position="20"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="326" mass="36150" checksum="8265486EF48E752F" modified="2005-09-27" version="1">MKKMLILCLFIISVLFSNAVVSIDDNASRQYILIGTGSMTGVYYPIGGSICRFITSDYKDSSNKVICSISSTTGSVYNLNSIRYSNMDVSIVQSDLEYYAYNGLGFYEKMLPMENLRMLASLHREYLTIVVRKNSNISVIDDIKGKRINIGSPGTGVRVAMLKLLNEKGWTRKDFSVMAELKSSEQAQALCDNKIDVMVDIIGHPNASIQEASATCDIMFVPLDDKLIDDLHAKYPYYQKDVINGSLYNSSSNINTVSVKASLVATTDLSDDLAYKIVKSMVTHLQELRGVTGALKTITVKDMARADITPMHDGAKRYYKEIGAIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS71</accession>
    <name>FENR_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ferredoxin--NADP reductase</fullName>
            <shortName evidence="1">FNR</shortName>
            <shortName evidence="1">Fd-NADP(+) reductase</shortName>
            <ecNumber evidence="1">1.18.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Ecaj_0391</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + NADP(+) + 2 reduced [2Fe-2S]-[ferredoxin] = NADPH + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:20125"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ferredoxin--NADP reductase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304512.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS71"/>
    <dbReference type="SMR" id="Q3YS71"/>
    <dbReference type="STRING" id="269484.Ecaj_0391"/>
    <dbReference type="EnsemblBacteria" id="AAZ68434">
        <property type="protein sequence ID" value="AAZ68434"/>
        <property type="gene ID" value="Ecaj_0391"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0391"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_5_5"/>
    <dbReference type="OMA" id="PEKVIYD"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01685">
        <property type="entry name" value="FENR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022890">
        <property type="entry name" value="Fd--NADP_Rdtase_type_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000364829" description="Ferredoxin--NADP reductase">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01685"/>
        </source>
    </evidence>
    <sequence length="338" mass="37478" checksum="5CE202B200DB2AA1" modified="2005-09-27" version="1">MTDYTTDIAVIGAGPVGIFTVFQAGMLKMQCCVIDALTEVGGQCTALYPEKPIYDIPGYPIVSGKELIDNLKKQSEPFNPQYLLGQIAEKIEDYSDYFLIRTSKGIVIQSKVIVIAAGAGAFGPNRPPIDNILDYENKSVFYQVKNISDFHNKNIMIAGGGDSAADWAVELSKIASQLYIVHRRRNFRCAPNTALQIDNLSQNGKINVIVPYQIKKLLGEEGKLHSVVVQNITNHEEITLQVDYLFPFFGTSANLGSMLNWGIEIKGYQMTVDFETCRTSRDRIYAVGDIATYPGKVKLILTGFSEAAMACHHIYHIVYPNSPLNFQYSTSKGIPNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS72</accession>
    <name>Q3YS72_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304511.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0390"/>
    <dbReference type="EnsemblBacteria" id="AAZ68433">
        <property type="protein sequence ID" value="AAZ68433"/>
        <property type="gene ID" value="Ecaj_0390"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0390"/>
    <dbReference type="eggNOG" id="COG0312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_0_0_5"/>
    <dbReference type="OMA" id="LYRKSTF"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD_M" evidence="3">
        <location>
            <begin position="120"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="2">
        <location>
            <begin position="229"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="444" mass="48704" checksum="29D3D3663B0588FD" modified="2005-09-27" version="1">MDILNIAEDAVKKIKSMGYIGDVVIYQSDKLSLSQRLHKIDEVVQSKNCKIGIRVIVNNNQFACVSSNEFSNISELINQAISMAKLSTGDPYISLYNEFVDKVNLMSGNDLMILDRTTLTIDQCKKIIEDMENAALSYDSRIINSEGSSFSNGITNVVLVTSNGFIGSYSKSNFVSNIAIIAGMDDKMEVGYDFSCVCNFNDIKNPIEIGKEAASRAINRLNPRKVQTSKMPVIIENRIASTLLGNFASAIRGDNIVNETSFLNGSINKKVFPDDIFIIDDPLMMCGLSSRPFDGEGVVGSKKFVVENGVLKSWILDIRSANKLKLKSTGNAIRHPNASITPGVSNFYIQNGNVSVEELMSDIKKGLYITDLFGFGINLITGDYSQGASGFFIENGKISYPVNEITIASKLQYMFAEMYVANDLQFLGSINSPTVRINDVTIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS81</accession>
    <name>Q3YS81_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0382"/>
    <dbReference type="EnsemblBacteria" id="AAZ68424">
        <property type="protein sequence ID" value="AAZ68424"/>
        <property type="gene ID" value="Ecaj_0382"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0382"/>
    <dbReference type="HOGENOM" id="CLU_1872170_0_0_5"/>
    <dbReference type="OMA" id="DIYTYHI"/>
    <dbReference type="OrthoDB" id="1760811at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="134" mass="15542" checksum="8541EC7A936586B5" modified="2005-09-27" version="1">MTEITILNSIIKLLKSNNNLMSIVNNVYDIIPNKVSLPYIHIYTSNLKTLNAFNNNMSKIQLLCKIYCYNSDTLNKILTIIINLLAHYKSNTNDLKYKIIPEYTYNIHQNNDIIYSLLNFTILAEKTHVNTITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS90</accession>
    <name>Q3YS90_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304493.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0372"/>
    <dbReference type="EnsemblBacteria" id="AAZ68415">
        <property type="protein sequence ID" value="AAZ68415"/>
        <property type="gene ID" value="Ecaj_0372"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0372"/>
    <dbReference type="HOGENOM" id="CLU_1084767_0_0_5"/>
    <dbReference type="OMA" id="SICSHIG"/>
    <dbReference type="OrthoDB" id="1780463at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="258" mass="29400" checksum="6C6FADF154BB51FD" modified="2005-09-27" version="1">MKNITKSKSFSYEKFHLISIAIILLLWNIFFTIKLISSISQHTKNLILPYTAILSISSILLYGINIAYNLNRIIKHFQEKEKLPKQRLVSFIGIIGEGVSLLGINIISTIGILIFPFNNYMLIASSIFSMLSSVFIIEYASIFLNTDIKQYKKLKDSNKHTKYTVWSMANWVGALMISIANISFISTSYLIKHDGSDIFKIVLFSVYVSIIISLLFLKSAQEKDNLTSLITETKSTQLNKKEEETTHIKSLECPNYSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS98</accession>
    <name>Q3YS98_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deoxyguanosinetriphosphate triphosphohydrolase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0364</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dGTPase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304485.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0364"/>
    <dbReference type="EnsemblBacteria" id="AAZ68407">
        <property type="protein sequence ID" value="AAZ68407"/>
        <property type="gene ID" value="Ecaj_0364"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0364"/>
    <dbReference type="eggNOG" id="COG0232">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028163_1_0_5"/>
    <dbReference type="OMA" id="HCKAFRR"/>
    <dbReference type="OrthoDB" id="370035at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016793">
        <property type="term" value="F:triphosphoric monoester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01212">
        <property type="entry name" value="dGTPase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006261">
        <property type="entry name" value="dNTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023023">
        <property type="entry name" value="dNTPase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026875">
        <property type="entry name" value="PHydrolase_assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13286">
        <property type="entry name" value="HD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01353">
        <property type="entry name" value="dGTP_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="64"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="397" mass="46243" checksum="57932E0686DCA957" modified="2005-09-27" version="1">MLDLVKYACNPVETRGRVFHEEEDQYCNCYQVDRDRIVYSGAFRKLQYKTQVFINYENDYYRTRLTHSLEVAQIARSLSRKLRFNEDLAEAISLAHDLGHPPFGHAGEEALNEMAMHHLGFDHNIQALRILTLLEKRYVKFNGINLTWETLEGVAKHNGPITGENRVNSSKKIHKFILDYDSEYRLDLDNFSSAEAQIAAISDDIAYNIHDIDDGIRAKILVIDELLELPLIGEVLKKVIDDNSGLSVSDNRIMHEFLRKTINIMMADIISQVTTNIKEYNISSQDDIRKLGKIFVHFSAEMNQYKVSLQNFLRAKLYNYYKVKRVKNKVKRIIKELFQVFYDDPQILPFDWSVKASDANLIDRSIVICDFISGMTDRFAIQEHRKIFDTTYEMLSF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSA1</accession>
    <name>NUOI_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit I</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoI</name>
        <name type="ordered locus">Ecaj_0361</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304482.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSA1"/>
    <dbReference type="SMR" id="Q3YSA1"/>
    <dbReference type="STRING" id="269484.Ecaj_0361"/>
    <dbReference type="EnsemblBacteria" id="AAZ68404">
        <property type="protein sequence ID" value="AAZ68404"/>
        <property type="gene ID" value="Ecaj_0361"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0361"/>
    <dbReference type="eggNOG" id="COG1143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067218_5_1_5"/>
    <dbReference type="OMA" id="RGDLYYT"/>
    <dbReference type="OrthoDB" id="1619561at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="PTHR10849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000250902" description="NADH-quinone oxidoreductase subunit I">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="1">
        <location>
            <begin position="59"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="1">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01351"/>
        </source>
    </evidence>
    <sequence length="167" mass="19500" checksum="2C107E1842A2F86F" modified="2005-09-27" version="1">MICKKKFLSPIINLFHSIIINCKGMYITLRYMFKPKVTLNYPLEKGPLSTRFRGEHALRKYKNGEERCIACKLCEAICPAQAITIEAQERDDGSRRTVRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEYATETREELMYNKSKLLHNGQVWEEAIDFRIKKNSQFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSA2</accession>
    <name>Q3YSA2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304481.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68403">
        <property type="protein sequence ID" value="AAZ68403"/>
        <property type="gene ID" value="Ecaj_0360"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0360"/>
    <dbReference type="HOGENOM" id="CLU_168862_0_0_5"/>
    <dbReference type="OMA" id="MNARIVN"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="116" mass="13608" checksum="E454EBBD2A65C13A" modified="2005-09-27" version="1">MFILSDIIEIRSQIVNIINIQIKYLEQSDFATVKDLKCIEKELINLLNCKHKKIKSSINVILSYKDQETIELLNNGYLDYRRILEIRNNILISKYTNKLLKSAVKPMNARIVNNLQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSB0</accession>
    <name>GATA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="1">Glu-ADT subunit A</shortName>
            <ecNumber evidence="1">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatA</name>
        <name type="ordered locus">Ecaj_0352</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAZ68395" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68395.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_044261964.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSB0"/>
    <dbReference type="SMR" id="Q3YSB0"/>
    <dbReference type="STRING" id="269484.Ecaj_0352"/>
    <dbReference type="EnsemblBacteria" id="AAZ68395">
        <property type="protein sequence ID" value="AAZ68395"/>
        <property type="gene ID" value="Ecaj_0352"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0352"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_5"/>
    <dbReference type="OrthoDB" id="1239251at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241098" description="Glutamyl-tRNA(Gln) amidotransferase subunit A">
        <location>
            <begin position="1"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="1">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="487" mass="53223" checksum="B9713DB9B7DA654F" modified="2006-06-27" version="2">MKDILKLSIAEMHDKLKKREFSAVELTKLHIEAVDNEKLNAFVTKTPEIALSAAEKADHIFTHQQENLTPLTGIPVGIKDLFCTKNVRTTACSNILKNFIPQYDSTVTKLLLNNGAVMLGKLNMDEFAMGSSNSNSCFGNVKNPWIRADGAEVVPGGSSGGSSAAVAGFLCAGALGSDTGGSVRQPAAFCGIVGLKPTYGRCSRLGMIAFASSLDQAGVLTRTVEDSALMLQSICGYDTQDPTSVNINVPKFSESITHKIKGTRIGIPKEYELSEKHKEYAEISEMWSKGIQYLKDEGAEIIEISLPHTSYALPVYYIICSAEASSNLARYDGIRYGTRISSDDINEMYKLTRGYNFGTEVKRRILIGAYALSSGYYDAYYNKAQCIRRLVTNDFIESFKKIDYILTPTAPKEAFSINEQLDTLTMYLNDVFTVPASLAGLPAISVPIGLSKNNLPLSLQIIGNYYDEGGILNLASIIEKHTGRILK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSB1</accession>
    <name>Y351_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable transcriptional regulatory protein Ecaj_0351</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Ecaj_0351</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TACO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304472.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSB1"/>
    <dbReference type="SMR" id="Q3YSB1"/>
    <dbReference type="STRING" id="269484.Ecaj_0351"/>
    <dbReference type="EnsemblBacteria" id="AAZ68394">
        <property type="protein sequence ID" value="AAZ68394"/>
        <property type="gene ID" value="Ecaj_0351"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0351"/>
    <dbReference type="eggNOG" id="COG0217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062974_2_2_5"/>
    <dbReference type="OMA" id="VQYVEGN"/>
    <dbReference type="OrthoDB" id="1304328at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00693">
        <property type="entry name" value="Transcrip_reg_TACO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="PTHR12532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="SSF75625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01033">
        <property type="entry name" value="TIGR01033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000257060" description="Probable transcriptional regulatory protein Ecaj_0351">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="248" mass="28050" checksum="0B5367B23A65E6B8" modified="2005-09-27" version="1">MAGHSQFANIKHRKGAQDAKRAQRFTKLIREIIVSAKQGLPDPEFNSRLRSAIIAAKKENLPKDRIDAAIKSATGNTQSDNYEEVVYEGYGPGNIALMIQTLTNNRNRTAAELRHALSKYNGKLGESGSVSFLFNHVGVIAYKAASIDNFDSLFNIAIELQALDVEETTQDESQEKMYYVTCNVQDFGNVRDQLFNKFSDAEFSRLFWKPSDILKIEDEEIQKKISSLMDLLENNDDVQYIDSNFTFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSD2</accession>
    <name>Q3YSD2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="6">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">coaD</name>
        <name evidence="8" type="ordered locus">Ecaj_0329</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the bacterial CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304451.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0329"/>
    <dbReference type="EnsemblBacteria" id="AAZ68373">
        <property type="protein sequence ID" value="AAZ68373"/>
        <property type="gene ID" value="Ecaj_0329"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0329"/>
    <dbReference type="eggNOG" id="COG0669">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100149_0_1_5"/>
    <dbReference type="OMA" id="EFQMALM"/>
    <dbReference type="OrthoDB" id="1846503at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00355"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02163">
        <property type="entry name" value="PPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00151">
        <property type="entry name" value="PPAT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001980">
        <property type="entry name" value="PPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF1">
        <property type="entry name" value="PTHR21342:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01020">
        <property type="entry name" value="LPSBIOSNTHSS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01510">
        <property type="entry name" value="coaD_prev_kdtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="7">
        <location>
            <begin position="5"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="124"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="162" mass="18432" checksum="28AF5780987D35A1" modified="2005-09-27" version="1">MKVGIYPGTFDPITFGHIDIIKRAYNLVDKLIIGVAKNCVKNTIFSAEVRAELIQHEIQLLNIAVETIIFDGLLVYFAQENNASVIIRGLRAVSDFDYEFQMSWVNYKLTPQIETIFLPASEDTQFISSSFVKEIARLNGDINAFVPISVQKYLKNFYQNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSD6</accession>
    <name>Q3YSD6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304447.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0325"/>
    <dbReference type="EnsemblBacteria" id="AAZ68369">
        <property type="protein sequence ID" value="AAZ68369"/>
        <property type="gene ID" value="Ecaj_0325"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0325"/>
    <dbReference type="eggNOG" id="COG3814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106715_1_0_5"/>
    <dbReference type="OMA" id="QMTIVIQ"/>
    <dbReference type="OrthoDB" id="1620073at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007481">
        <property type="entry name" value="SspB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036760">
        <property type="entry name" value="SspB-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04386">
        <property type="entry name" value="SspB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101738">
        <property type="entry name" value="SSF101738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="157" mass="18195" checksum="E40998D2DC4856EC" modified="2005-09-27" version="1">MSNAIDYQKLMHSAMCTVVKNALTVIAHSEHSTNVHIAISFLTNYKGVTLPDHIKENYPQEITVILQHQFRSLQVFDNSISVILSFRGQEETVVIPFHSIIKYIDVYQGFVLDLEQYMSNNIEEADNYDYDTDDKNEESDQQNNQDNIIFIDTFLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-04-07" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSE2</accession>
    <name>Q3YSE2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0319</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304441.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0319"/>
    <dbReference type="EnsemblBacteria" id="AAZ68363">
        <property type="protein sequence ID" value="AAZ68363"/>
        <property type="gene ID" value="Ecaj_0319"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0319"/>
    <dbReference type="eggNOG" id="COG3437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_51_5"/>
    <dbReference type="OMA" id="INCIFTS"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="446"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="706"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="824" mass="94091" checksum="3A594125011F2462" modified="2005-09-27" version="1">MRKRHTDNTHDNKVDFVIRRYGLSVCKLSIFLIAPLLCILSLYIFNIYDSYINIIINCIFTSISILAISIQLRQYLHIIASIEYQNMIFANSLNHNTEFCLIIKNDGKIIYSDARFYERFIKNQETKLNLFDILKIGNISEQEIETFKNALKNKLSINTYFCLNTKNKISNFTLILDPVSENPEIEINYSKKINLFFAPLARPQEYFVLKAIKITKEQVYERLIQKHCVGAYLLNHRGVILSANDSFLKMLELNSIENGTTLTNFLVKGYNKDKIIDNNTVTLSTSTGIKFKAHISQAAFYDKNNHSYIYGLLTPVNSDILNYHLHPCFTEAPIAILQCSTDGKILKSNNTLKRLVKHSKDYIFEYILPSYNKKIKKYFQNNIVKNMSLEAQLYNNLYVKIHFNKFIHQNNMFIICYITDSEDRKNLEIQLEQSQKMQAIGQLAGGIAHDFNNILTAIIGFCDLLLIRHTATDPSFSDIMQIKQNANRATNLIKQLLAFSRKQTLQPKILDVNNIIADLLHMIKRLINESIELKIQYEQNINLVKADLCQLEQVIINLVVNARAAMETGGQLSIKTYNTKTDALKILLKNMFSPDKEQIEDGEYVAIEISDTGYGMEDKIMKKIFDPFFSTKETASGIGLGLSTVYGIVKQTDGYIYVKSTVNVGTTFIILIPTVHLSNSNDIEIPYNKYSQEVNNVIPQVSTSTTILLIEDEDPVRIFATKALTKKGFKVIDISCGDQALDIINKYPIDIVISDVVMPKISGPEIVEQILKAKPDIQIIFISGYAEEVFNKHSNIDATKINFLSKPFTLKQLTEKVLEISEHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSK4</accession>
    <name>Q3YSK4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0254</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304379.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0254"/>
    <dbReference type="EnsemblBacteria" id="AAZ68301">
        <property type="protein sequence ID" value="AAZ68301"/>
        <property type="gene ID" value="Ecaj_0254"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0254"/>
    <dbReference type="eggNOG" id="COG5449">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080134_0_0_5"/>
    <dbReference type="OMA" id="WLVNWAD"/>
    <dbReference type="OrthoDB" id="756610at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011928">
        <property type="entry name" value="Phage_phiJL001_Gp84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018964">
        <property type="entry name" value="Phage_phiJL001_Gp84_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019228">
        <property type="entry name" value="Phage_phiJL001_Gp84_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09931">
        <property type="entry name" value="DUF2163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09356">
        <property type="entry name" value="Phage_BR0599"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02218">
        <property type="entry name" value="phg_TIGR02218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2163" evidence="2">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Phage_BR0599" evidence="1">
        <location>
            <begin position="191"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="269" mass="30746" checksum="2CF9476872C9CAC2" modified="2005-09-27" version="1">MKNINPKLQQHLSSEILTIAHCIQIILQNKKIIGLTNFDQNLHIDNITYEATPELIIDTLKYNFNYGNSTKIETTINNNIIKEEIISGLYDSAIVKIFLVNYTDLTQGSVILFYGNIDEITLINDKLIAELSSLYNILSRNIGDSFSSSCRAQFCDNKCKLNKEEFTNIYSITRVISDQEFECINLINIDNYYTYGFITFIGGENNNSTVEVKSHNQAYIKFYNTLPKKISVHDTFSIVAGCDKSFTTCSNRFNNSKNFRGEPHIPEIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSK7</accession>
    <name>Q3YSK7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator family transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0251</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304376.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0251"/>
    <dbReference type="EnsemblBacteria" id="AAZ68298">
        <property type="protein sequence ID" value="AAZ68298"/>
        <property type="gene ID" value="Ecaj_0251"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0251"/>
    <dbReference type="eggNOG" id="COG0738">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_62_1_5"/>
    <dbReference type="OMA" id="LRDIWIT"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="284"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="7"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="401" mass="44868" checksum="C81C38AA0789225E" modified="2005-09-27" version="1">MCFYPIFNWIIGVLVLVCGGNFQIAYSVINPYVSEDLNLNIAQTALAASIHMWSFSVFQLFTGSVLDYYDAKKVLPISIIITSIGTFLFSYSDYTICFFVSQVFLGLGASFGFVGAGYISYRFFSPLKSGIMFGLVQTVYSLSSLLMEYTYTYLTSSGLSWRDIIKYIEYAEIVVLILTLFLVSSKKSIYLKNNSLSISSVLLQVFKHIVEVVRVKNIWLFTIVGSLMFGVFLSIAILWGQKLLMAMNLDSYYSGVVHSIIWIGFAIGCPLVNTISNALKNRKYVFMGFCLLQCVSLTLLLYSNNVYLLCFLIFIFGCASGGHMLNFSIGTDVVDESKVGTVCSIINGLMCIVGGVIMFLVGLALEYQNKYDIIHISLSMPILIFLSFCVLFFCRDTFSMK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSL1</accession>
    <name>RECR_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Recombination protein RecR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">recR</name>
        <name type="ordered locus">Ecaj_0245</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304372.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSL1"/>
    <dbReference type="SMR" id="Q3YSL1"/>
    <dbReference type="STRING" id="269484.Ecaj_0245"/>
    <dbReference type="EnsemblBacteria" id="AAZ68294">
        <property type="protein sequence ID" value="AAZ68294"/>
        <property type="gene ID" value="Ecaj_0245"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0245"/>
    <dbReference type="eggNOG" id="COG0353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060739_1_1_5"/>
    <dbReference type="OMA" id="DVMAIEN"/>
    <dbReference type="OrthoDB" id="1661836at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01025">
        <property type="entry name" value="TOPRIM_recR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00017">
        <property type="entry name" value="RecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000093">
        <property type="entry name" value="DNA_Rcmb_RecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023627">
        <property type="entry name" value="Rcmb_RecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023628">
        <property type="entry name" value="Rcmb_RecR_C4-type_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015967">
        <property type="entry name" value="Rcmb_RecR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034137">
        <property type="entry name" value="TOPRIM_RecR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30446">
        <property type="entry name" value="PTHR30446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02132">
        <property type="entry name" value="RecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111304">
        <property type="entry name" value="SSF111304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00615">
        <property type="entry name" value="recR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01300">
        <property type="entry name" value="RECR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000322887" description="Recombination protein RecR">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="76"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00017"/>
        </source>
    </evidence>
    <sequence length="195" mass="21520" checksum="E5BF2A93AE45399B" modified="2005-09-27" version="1">MDINKLINMFAKLPNFGPSSSRRIVLHLLRNKEEVMLPLASGIQDLAFQTKECSVCFNIDVKSPCSICSDTKRDHQLLCIVEELGDLWAFEKGKIYQGVYHVLGGTLSAIYGIGPDQLNLDNIVERIKKFDIKEVIIGIGNTMDGQVTTHYITQIVKGLGIKITRLACGIPMGGEIDYLDEGTLSAALSSRYVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSM7</accession>
    <name>Q3YSM7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304356.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0229"/>
    <dbReference type="EnsemblBacteria" id="AAZ68278">
        <property type="protein sequence ID" value="AAZ68278"/>
        <property type="gene ID" value="Ecaj_0229"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0229"/>
    <dbReference type="HOGENOM" id="CLU_789246_0_0_5"/>
    <dbReference type="OMA" id="SHCNQAG"/>
    <dbReference type="OrthoDB" id="1747881at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="354" mass="40591" checksum="7B12523917814805" modified="2005-09-27" version="1">MNDNTKIIIAGLAIAFLIFTILLIYILTKYLKHHNNIPFDICIRLPEASIKKSMFALTCEKQPNTNAIKGNTSHRDFTGIKYYINDIQININDFSKNTLILKKMLKNGIKQTDNLYYRLIMKHKEVIIQALNISDKTCYNIFQNRIVMAAIFEAAFKSITNTTPSINIIHELVSHCNQAGNFVMPGEFLTTIFKKCKDFFPSTHSKNNKASFTIENEEEITCCIASDIEMYKYADTKSEISEKSTVSSLQGKLTFTISSKDCSNFSHTLYKDITLEIIVPEEMKKYITTSDITNESYNSNITLINRTKTVDNETRLIYSLPNLNIPSLINRKNLDFILRNNSISIEHCETLKQH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSN4</accession>
    <name>RL33_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmG</name>
        <name type="ordered locus">Ecaj_0222</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAZ68271" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68271.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304349.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSN4"/>
    <dbReference type="SMR" id="Q3YSN4"/>
    <dbReference type="STRING" id="269484.Ecaj_0222"/>
    <dbReference type="EnsemblBacteria" id="AAZ68271">
        <property type="protein sequence ID" value="AAZ68271"/>
        <property type="gene ID" value="Ecaj_0222"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0222"/>
    <dbReference type="eggNOG" id="COG0267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190949_1_0_5"/>
    <dbReference type="OrthoDB" id="2034291at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00294">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="L33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001705">
        <property type="entry name" value="Ribosomal_L33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00471">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01023">
        <property type="entry name" value="rpmG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000356453" description="50S ribosomal protein L33">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="56" mass="6498" checksum="2BF147BA729BBEFE" modified="2008-12-16" version="2">MAKKGSTLLVKLASSAKTGFFYVKKRNPKKLINKLSFRKYDPVVRKHVLFSEEKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSN7</accession>
    <name>Q3YSN7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acyl carrier protein</fullName>
            <shortName evidence="8">ACP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">acpP</name>
        <name evidence="11" type="ordered locus">Ecaj_0219</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304346.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0219"/>
    <dbReference type="EnsemblBacteria" id="AAZ68268">
        <property type="protein sequence ID" value="AAZ68268"/>
        <property type="gene ID" value="Ecaj_0219"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0219"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108696_5_1_5"/>
    <dbReference type="OMA" id="FISKMNF"/>
    <dbReference type="OrthoDB" id="1943389at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="UniPathway" id="UPA00360"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036104">
        <property type="term" value="P:Kdo2-lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="Acyl_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="PTHR20863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1 8 9" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="10">
        <location>
            <begin position="9"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="8 9">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="91" mass="10309" checksum="4E42F8E3AD9AD455" modified="2005-09-27" version="1">MESNDGKLESIKDTVTKIVIDCLKLKDEQKATLSGSTNLAKDLNLDSLDFVDLVMCLEDHFSIEISDEEAQELETIDKIEEYIRNKISSKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSQ2</accession>
    <name>CLPX_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease ATP-binding subunit ClpX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clpX</name>
        <name type="ordered locus">Ecaj_0203</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the ClpX-ClpP complex. Forms a hexameric ring that, in the presence of ATP, binds to fourteen ClpP subunits assembled into a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpX chaperone family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304331.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSQ2"/>
    <dbReference type="SMR" id="Q3YSQ2"/>
    <dbReference type="STRING" id="269484.Ecaj_0203"/>
    <dbReference type="EnsemblBacteria" id="AAZ68253">
        <property type="protein sequence ID" value="AAZ68253"/>
        <property type="gene ID" value="Ecaj_0203"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0203"/>
    <dbReference type="eggNOG" id="COG1219">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014218_8_2_5"/>
    <dbReference type="OMA" id="HYKRVQA"/>
    <dbReference type="OrthoDB" id="718259at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00175">
        <property type="entry name" value="ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004487">
        <property type="entry name" value="Clp_protease_ATP-bd_su_ClpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046425">
        <property type="entry name" value="ClpX_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010603">
        <property type="entry name" value="Znf_CppX_C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038366">
        <property type="entry name" value="Znf_CppX_C4_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06689">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00994">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00382">
        <property type="entry name" value="clpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51902">
        <property type="entry name" value="CLPX_ZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000024553" description="ATP-dependent Clp protease ATP-binding subunit ClpX">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="ClpX-type ZB" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="114"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01250"/>
        </source>
    </evidence>
    <sequence length="406" mass="44827" checksum="D571DBF23E91F921" modified="2005-09-27" version="1">MADSEKNSCSCSFCGKIHSEVRKLIAGPSVFICNECIDLCSGILQEEGRSYKKTDTLDLKPKEIKKVLDEYVIGQEHSKKVLSVAVYNHYKRLSNSGVISEVEISKSNVLLIGPTGSGKTLLARTLARVLQVPFAMADATTLTEAGYVGEDVENILLKLLQAANFNVDAAQRGIIYIDEVDKISRKSENTSITRDVSGEGVQQALLKVIEGTVSSVPPQGGRKHPHQEFIQINTDNILFIFGGAFDGLDKIIESRHRGSSMGFEANVQKVSKNKDIFCYTEPEDLVKFGLIPEFVGRIPVITSLSELNESTLCRILVEPKNSLVKQYKKLFEMDNIDLQFDDSALSEIAKKAAVRKTGARGLRAILEALLLDLMFESPGDVGINQVVISKKMVEELMVNSRLFLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSR4</accession>
    <name>Q3YSR4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Succinate dehydrogenase hydrophobic membrane anchor subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Ecaj_0190</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Membrane-anchoring subunit of succinate dehydrogenase (SDH).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur protein, plus two membrane-anchoring proteins, SdhC and SdhD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell inner membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0190"/>
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        <property type="protein sequence ID" value="AAZ68241"/>
        <property type="gene ID" value="Ecaj_0190"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0190"/>
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        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151315_0_2_5"/>
    <dbReference type="OMA" id="RIFTITC"/>
    <dbReference type="OrthoDB" id="2048140at2"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000104">
        <property type="term" value="F:succinate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
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    <dbReference type="InterPro" id="IPR014312">
        <property type="entry name" value="Succ_DH_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="SSF81343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02968">
        <property type="entry name" value="succ_dehyd_anc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAZ68241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="118" mass="13625" checksum="CB460FBF7940035B" modified="2005-09-27" version="1">MVSHSVYHWLIQRATAFVLLPLSMWFLFKFVAVVSLIFKSLPDLHLCFSSIGITDLVVLLCFFVFAFYHGVLGIQVILEDYIHSSILRTSVFLVIKVIVVLTVLFLVFIVLYNTLFMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSU7</accession>
    <name>Q3YSU7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Beta-lactamase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0157</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0157"/>
    <dbReference type="EnsemblBacteria" id="AAZ68208">
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        <property type="gene ID" value="Ecaj_0157"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0157"/>
    <dbReference type="eggNOG" id="COG0595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008727_3_3_5"/>
    <dbReference type="OMA" id="KNTCVFE"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004613">
        <property type="entry name" value="RNase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041636">
        <property type="entry name" value="RNase_J_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17770">
        <property type="entry name" value="RNase_J_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00649">
        <property type="entry name" value="MG423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="8">
        <location>
            <begin position="22"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="544" mass="60634" checksum="F21CA94A9A19F7D7" modified="2005-09-27" version="1">MCLKMNTKDLLFVPLGGVGQIGMNVNLYHLDGKWIIIDFGAGFADDNMPGIDMIVADISFIKKNRKNLLGIVLTHAHEDHIGGIQYLWSDLQCPIYATKFTSALLQAKLKEYQLKVNITEIDITKRLKLGPFSLEFINLTHSIPEMNAIALHTEKGVIVHTGDWKLDDNPIIGPISNTQRLKELGDEGVLALVCDSTNIFTKNKSGSEGDLEESLFNIIKDCKQKVAVSLFASNIARIQTIVHIAEKLGRKVVILGKSLVRITQAAQGSGYLQDISQFIDINQANKLPRNQVLLLCTGCQGEALAATAKLANNSHSLTKLEHGDTIIFSSKIIPGNEKRIYSVFNKFINMGVNVITEFMEYVHVSGHPSKIEVSTMYSLIRPKLSIPVHGEYIHMHEHAKVAEQCNVEKAIIVHPGDVINIIKKQKVNSVKAGYFGVDGNFLHHPQGNVISMRKRMRDSGIIIVTLVLNNKKALLKEPRIFAPGVLDHSDNQAITKKILSKISSDLYSKKINNIKQHVENIVFNALKYDIKNKPFIEVQLEYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSU9</accession>
    <name>Q3YSU9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Membrane fusion protein (MFP) family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0155</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304284.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0155"/>
    <dbReference type="EnsemblBacteria" id="AAZ68206">
        <property type="protein sequence ID" value="AAZ68206"/>
        <property type="gene ID" value="Ecaj_0155"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0155"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023976_1_1_5"/>
    <dbReference type="OMA" id="FPIVPGM"/>
    <dbReference type="OrthoDB" id="1395473at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010129">
        <property type="entry name" value="T1SS_HlyD"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01843">
        <property type="entry name" value="type_I_hlyD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="505" mass="56920" checksum="AECE7399BB6C7F92" modified="2005-09-27" version="1">MYKKLKDFIKVGFSLPKLLGHNTLHIENNVEEQMSSTLTKSLSIVDNIVNFLFKYNRKGTINEVLKVSWGPLFSGFIIILLFFGVFGIWAAVAPLDGSVIAQGEVISSSEKQIVQHLEGGLIDKILVKEGELVQKNQPIIYLHNTTAQANLDIIKERMLDLLATEARLIAIKYDRESIDFPNTIKNLSDEKTRDQIIQNQQQLFESHKKNISGQIDILQQRTKQLRQELSGLSAQLTSATTQHKLISEELETKKNLLETGYISKPYIIALERQYAESEGKIGQLKSAIASVNQKIGENKLEIINITNNVQNKINLDLKDITSAITDLKERMKTAQDILDRTTIRSPHAGVITGLKYHTEGGVIPPGAPITNVVPLNDDLVIDAKIQTRHIEEILSAQMKDKNIVSYEKYRGLKAKVRLSAYNIRKIGLVNGIVVQVSADALSEINGMRYYKIQVIIPKEQSEKLRNLKLYPGMPAEVFIITQSRTLLNYLFTPITSTFEKAFNER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSV6</accession>
    <name>SUCC_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Succinate--CoA ligase [ADP-forming] subunit beta</fullName>
            <ecNumber evidence="1">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="1">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sucC</name>
        <name type="ordered locus">Ecaj_0148</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304277.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSV6"/>
    <dbReference type="SMR" id="Q3YSV6"/>
    <dbReference type="STRING" id="269484.Ecaj_0148"/>
    <dbReference type="EnsemblBacteria" id="AAZ68199">
        <property type="protein sequence ID" value="AAZ68199"/>
        <property type="gene ID" value="Ecaj_0148"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0148"/>
    <dbReference type="eggNOG" id="COG0045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037430_0_2_5"/>
    <dbReference type="OMA" id="ITACDEV"/>
    <dbReference type="OrthoDB" id="316012at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_synthase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="PTHR11815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_1000082076" description="Succinate--CoA ligase [ADP-forming] subunit beta">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="1">
        <location>
            <begin position="9"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="320"/>
            <end position="322"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00558"/>
        </source>
    </evidence>
    <sequence length="386" mass="42168" checksum="F3A39C8DAA9B3BDE" modified="2005-09-27" version="1">MNIHEYQAKHILSRFGVSVPKGAIVHSLSEVDDAISKLHSKVIVVKAQIHAGGRGKAGGVVVCRTSDEAKASIKNMLGSTLVTHQTSKDGQKVRKVYLEEGCDIKKEYYISAIVNRKQSQVSIIFSTEGGVDIEEVASTSPEKVIVCNIDPLFGFQSFHGRNLCFDSNLSLDQTRKISDIAEKIYKVMLSTDANQVEINPLVETSSGNFIALDAKINFDDNALYRHPDIQELRDYDEEIKEEIEASKYGLNYIKMDGNIGCMVNGAGLAMATMDIIKYYGAEPANFLDVGGGASQKTVTEAFKIILSDKVDGILVNIFGGIMRCDIIANGIIAAIEEIRINVPLVVRLSGTNFELGKKLLDDSSLNIITANDLSEAAYNIVNIVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSW4</accession>
    <name>Q3YSW4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304269.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0140"/>
    <dbReference type="EnsemblBacteria" id="AAZ68191">
        <property type="protein sequence ID" value="AAZ68191"/>
        <property type="gene ID" value="Ecaj_0140"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0140"/>
    <dbReference type="HOGENOM" id="CLU_565889_0_0_5"/>
    <dbReference type="OMA" id="DYFQICV"/>
    <dbReference type="OrthoDB" id="328876at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="482" mass="55256" checksum="C504E634C216C9BE" modified="2005-09-27" version="1">MSFFSKMSKNRIVISVGDNGAVVLNIVNSEVRDKYFIENKQDVDLSKVVSCIIEYKTSSIYLVLNHSEQIYTEHVMPTYNRVVAKSLVKMNLNDVMGDYDISDAFLLTKPNDSNKSCHYMVVKSQLNNLTKKLLEVIVSNLGNFCGILLLPTELSYLCDRLLKFYGKDNEGWVILIAYTKTNDFRKIVLYQGKIIHVSNVIITSDETLPSIIAGKIYQEISNTVLYLAKFGYSKESIINLYIITSSNIKTSLLSFDFKNMDSSILTPYELSKILALKQSSSVVSEFCDTVVLCAITEKTPLKVFHTKDTSLFYKILFFNKYVLPCLLCFIALLSLLNITYMFSINDNYDEKIDLLLRKEELVSKVEKISQDTTLKQVNEMYETVDVYKLLLADDFFSLDLILRLRKVDLKGFNVNYLSCNARGHGIAVKLLLKFVEEKQFSYYFNGLKDRIMEEFKDYKTEVIDTLPNVADNEKIIVVKLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSW5</accession>
    <name>Q3YSW5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304268.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68190">
        <property type="protein sequence ID" value="AAZ68190"/>
        <property type="gene ID" value="Ecaj_0139"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0139"/>
    <dbReference type="HOGENOM" id="CLU_353647_0_0_5"/>
    <dbReference type="OMA" id="THELQQD"/>
    <dbReference type="OrthoDB" id="754492at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="717"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="794" mass="89125" checksum="AA3EE9A12F57C0B6" modified="2005-09-27" version="1">MDSFLNAYSVDLIQQNSTIFSLYYVKRGLVANDLICEVHHDNSNYAVKVNDVPILDHTVKNYLPRMLFVKGVDNDFLIVAVSVNELKQYKIRKSLLLKIYKINYLPTIALLDFDKINLSSSIRSLREEVKYPVVLYQNRPDTIMVIARVDNDDRGRSPDRLYVMWNLRYRDGRFAIIKPAASEGQFNNRYLFKYSGYIGYGKYSDKLIMVSEVKGHLKLLYVGKYLSQDYNFFSNIYEISVDYTLNFGARKCIINYRYECNLRDIDKYNIPVGGIDVINAISNGKSTYLAYVGVVSNSKFGDDTQLVIVSNKQGKQVTIYDFMRVADRVTSLYVVHVDSNIVMTTVSSSYIVRYEIPELQLRNGNVTHINIMQFSRREITNVANFANVITGNISFPSDKYINVTTVAAKKLCNSVENVYFNVRSILSGDIFTTEQSLSSVNASMYETTRSVISRSESTKMSVEPVTSVYMLSNTEKPVMAVDYPSEASSTVQSIISKSSITEVGIKSATANSLSYNSDSTMESVSTRDKFSTMSSAIQSIAPKSYALEREKGASTSSAIQAEIQGSSTTEDVKHLSSDTVSSIGFSTSQSVVRYYVTDTVNSLSIDRNDVVSSITDVTAHPITNKKKSKIVDNRSNVSDVKYGSLHKKVKYNVKRSTLKPGIKTSIKEHTFHSDFTPTDYVLANRLNLTNNVSMSDYYLTNNGTDESNVRMDNSKNGLLIGVVCAVFFIVMVLFFSKGGYDRLKRGVIRCLGNGRRTRCRSANPRVLPVLELHDLREISGVIISNRAAYASSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSY3</accession>
    <name>Q3YSY3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit M</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">NADH dehydrogenase I subunit M</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">NDH-1 subunit M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0121</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304250.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0121"/>
    <dbReference type="EnsemblBacteria" id="AAZ68172">
        <property type="protein sequence ID" value="AAZ68172"/>
        <property type="gene ID" value="Ecaj_0121"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0121"/>
    <dbReference type="eggNOG" id="COG0651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_9_0_5"/>
    <dbReference type="OMA" id="AFPVNFW"/>
    <dbReference type="OrthoDB" id="1887859at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="6">
        <location>
            <begin position="129"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00031584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="491" mass="54184" checksum="72AECDBEECB048FE" modified="2005-09-27" version="1">MISLLPILQVVIPLLSAVGCALLKNARLVKIISAIVIFVSFSIAIILFCQIYPNGVIKYSIGGWVVPYGIELKVNIFNATMLVLVGFVAVMSVIYGIYPNTKEVNVSTIPSFYSIFLLCFSGFLGILVSNDVFNIYVFLEISSISSYILVAMGRDKTALIAAFDYLVVGTIGATFYLIGVGFLYAITGTLNVGDLFLIVQDKSLATNKAIQIGLLFIAVGLFVKAALFPFHKWLIQAYNYAPTFVSVFFSGVSTKVMIYLVIKVVYDIFKAELVLITLPFNFVFMFFAALSVIFASVFAILSDNIKRIFAYSSVAHLGYIVFAVGLNTYYGLAAAVTYIINHSLVKSALFMVIGSISYNCGSLDLKDCTNIWKNMPKIALPFVILCISLIGMPITSVFISKWYIFDATIKSNFWFGMVMLLVGSGLSVIYVWNIIEATCFKSSDKQNKKLFEIPNIMVLCIWVIAISSIILGLYPMPLTLISNKIAESLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSZ4</accession>
    <name>Q3YSZ4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="8">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpS</name>
        <name evidence="10" type="ordered locus">Ecaj_0110</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304239.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0110"/>
    <dbReference type="EnsemblBacteria" id="AAZ68161">
        <property type="protein sequence ID" value="AAZ68161"/>
        <property type="gene ID" value="Ecaj_0110"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0110"/>
    <dbReference type="eggNOG" id="COG0180">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029244_1_4_5"/>
    <dbReference type="OMA" id="GWGQFKP"/>
    <dbReference type="OrthoDB" id="951354at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_B">
        <property type="entry name" value="Trp_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024109">
        <property type="entry name" value="Trp-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="12"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="197"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="11"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="19"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="197"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="333" mass="37227" checksum="67995BA09D2A60A9" modified="2005-09-27" version="1">MSTPIALSGIQPTGSIHLGNYLGSIRNWVSLQNSYKCFFFLADLHTLTSQSYNPAELKQNSINTLATYLACGIDPNKSVLFIQSSINAHSELLWILSCITSTGQLNRMTQFKEKSRNKASASCLGLYSYPVLMAADILIYKANIVPVGVDQKQHLELTRDIAISFNSTYNTQYFQIPEPLIVQESAKIMSLRNGKKKMSKSDSSDFTRINLYDSNDMISKKISKATTDSIMGFDFNTLDSRPEVKNLVSIYATLLNINIEQACANIENLDTKQFKEQLAELIISNVAPIQQKVKEFLEDQEHLHNIIVSGNNKAASIANKHILEIKEIVGCWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT03</accession>
    <name>Q3YT03_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome-binding ATPase YchF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ychF</name>
        <name evidence="8" type="ordered locus">Ecaj_0101</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304230.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0101"/>
    <dbReference type="EnsemblBacteria" id="AAZ68152">
        <property type="protein sequence ID" value="AAZ68152"/>
        <property type="gene ID" value="Ecaj_0101"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0101"/>
    <dbReference type="eggNOG" id="COG0012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018395_0_1_5"/>
    <dbReference type="OMA" id="VLRCFDN"/>
    <dbReference type="OrthoDB" id="603226at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00092">
        <property type="entry name" value="TIGR00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="6">
        <location>
            <begin position="3"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="7">
        <location>
            <begin position="277"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="362" mass="40205" checksum="B6FEB65A8D1D5E8E" modified="2005-09-27" version="1">MGLNCGIVGLPNVGKSTLFNALTQTMVAEVANYPFCTIEPNVGKAIVQDHRLKTLAQMASSKKIIYNQVECVDIAGLVSGASQGEGLGNKFLSHIREVDAIIHVLRCFSDQNISHVNQTVDPISDAEIVEMELILADIESLKRRLPALEKAVKSNKEPRKKLDNILEVLSILESGNLAKNAAHLGEDLKQLQLITTKPMMYVCNVEESNVTHGNALSEKVRLMAEKKHNKFCCISAKLEADVSSLETEEEKQIFLSEFNLEESGTTSVVKTMYDLLDMITFFTLGPQEARAWPIKRLSTASTAAGVIHTDFEKGFIKAELISFDDYIKYNGEAKCKEAGKVRFEGRDYIVQDGDIIHFRHNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT13</accession>
    <name>PTH_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="1">PTH</shortName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pth</name>
        <name type="ordered locus">Ecaj_0091</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304220.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT13"/>
    <dbReference type="SMR" id="Q3YT13"/>
    <dbReference type="STRING" id="269484.Ecaj_0091"/>
    <dbReference type="PRIDE" id="Q3YT13"/>
    <dbReference type="EnsemblBacteria" id="AAZ68142">
        <property type="protein sequence ID" value="AAZ68142"/>
        <property type="gene ID" value="Ecaj_0091"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0091"/>
    <dbReference type="eggNOG" id="COG0193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062456_1_0_5"/>
    <dbReference type="OMA" id="HVLSKFH"/>
    <dbReference type="OrthoDB" id="1676462at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:aminoacyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00462">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00083">
        <property type="entry name" value="Pept_tRNA_hydro_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PTHR17224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="SSF53178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01195">
        <property type="entry name" value="PEPT_TRNA_HYDROL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000264034" description="Peptidyl-tRNA hydrolase">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00083"/>
        </source>
    </evidence>
    <sequence length="193" mass="22044" checksum="7DB8C38E0738E805" modified="2005-09-27" version="1">MFHLLAGLGNPGTQYTLTRHNAGFMVIDAILDKFHFPNFKKKNNALISIGNIESYRVILVKPWTFMNNSGVPIMNIVSLYKIPLDNMIVFHDEVEIDFCTIRVKKSGGNAGHNGLKSIDNFLGKDYWRVRFGIGHPRNKMDLSDYVLSHFHNLNAVNNTISSIVEHITLLLEKNHTTFTDKVRNTLKYEDIPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT20</accession>
    <name>Q3YT20_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 8">Transcription elongation factor GreA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcript cleavage factor GreA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">greA</name>
        <name evidence="11" type="ordered locus">Ecaj_0084</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7 8">Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the GreA/GreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0084"/>
    <dbReference type="EnsemblBacteria" id="AAZ68135">
        <property type="protein sequence ID" value="AAZ68135"/>
        <property type="gene ID" value="Ecaj_0084"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0084"/>
    <dbReference type="eggNOG" id="COG0782">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101379_2_0_5"/>
    <dbReference type="OMA" id="TWLTQEA"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00105">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018151">
        <property type="entry name" value="TF_GreA/GreB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006359">
        <property type="entry name" value="Tscrpt_elong_fac_GreA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028624">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022691">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036805">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03449">
        <property type="entry name" value="GreA_GreB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006092">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46557">
        <property type="entry name" value="SSF46557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01462">
        <property type="entry name" value="greA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00829">
        <property type="entry name" value="GREAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="GreA_GreB_N" evidence="10">
        <location>
            <begin position="14"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GreA_GreB" evidence="9">
        <location>
            <begin position="90"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="170" mass="19454" checksum="EECD895DB80BCA47" modified="2005-09-27" version="1">MVWLMNNYMNERFPITKSGFEKLELELESLKHERPKIIKSISEARELGDLSENAEYHAARERQGLIESKIMELESKRSRAEVIDVSNLSGDTVMFGATVTMSIYDEQTGNTDEVVYKIVGEYEVDISKQMISIKSPLVLSLLGKKEGDVVEVRTPKGDYKIYKIIKIEFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT22</accession>
    <name>ATPD_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F(1) sector subunit delta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-type ATPase subunit delta</fullName>
            <shortName evidence="1">F-ATPase subunit delta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpH</name>
        <name type="ordered locus">Ecaj_0082</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">This protein is part of the stalk that links CF(0) to CF(1). It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase delta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304211.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT22"/>
    <dbReference type="SMR" id="Q3YT22"/>
    <dbReference type="STRING" id="269484.Ecaj_0082"/>
    <dbReference type="EnsemblBacteria" id="AAZ68133">
        <property type="protein sequence ID" value="AAZ68133"/>
        <property type="gene ID" value="Ecaj_0082"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0082"/>
    <dbReference type="eggNOG" id="COG0712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085114_1_2_5"/>
    <dbReference type="OMA" id="AQPYAKA"/>
    <dbReference type="OrthoDB" id="1937493at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020781">
        <property type="entry name" value="ATPase_OSCP/d_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="PTHR11910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="SSF47928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00389">
        <property type="entry name" value="ATPASE_DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000184703" description="ATP synthase subunit delta">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01416"/>
        </source>
    </evidence>
    <sequence length="183" mass="20356" checksum="5F63BD74096FD57A" modified="2005-09-27" version="1">MTQYRGGYIASCYAQALFSVSDVNSICKDVEFVISVLENDNNVAMFLSSPRVSKESKISLIKVIGDYIDSILVKFMIIVIESNRGNILLQIFSAFLDLVRKYNKEVNISVTSCALLTKQEEEGICNALLERYGKVVGITNNLDPSILGGFIIRVNFDVIDVSLNSYLQSLRELSKMSVCSISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT23</accession>
    <name>Q3YT23_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304210.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0081"/>
    <dbReference type="EnsemblBacteria" id="AAZ68132">
        <property type="protein sequence ID" value="AAZ68132"/>
        <property type="gene ID" value="Ecaj_0081"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0081"/>
    <dbReference type="eggNOG" id="COG5454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160698_3_0_5"/>
    <dbReference type="OMA" id="FFIISWW"/>
    <dbReference type="OrthoDB" id="2038848at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009935">
        <property type="entry name" value="DUF1467"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07330">
        <property type="entry name" value="DUF1467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="84" mass="9403" checksum="0DC62CC4B8B2FD6A" modified="2005-09-27" version="1">MGVGDIVFFIISWWIILFLILPIKVEVPDNPVIGSASSAPVKSYLLIKSIIATVTSALLTGLYVYLKIKGYIDFEYIYDLITFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT37</accession>
    <name>Q3YT37_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304196.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68118">
        <property type="protein sequence ID" value="AAZ68118"/>
        <property type="gene ID" value="Ecaj_0067"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0067"/>
    <dbReference type="HOGENOM" id="CLU_396260_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021902">
        <property type="entry name" value="DUF3514"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12027">
        <property type="entry name" value="DUF3514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="695" mass="78185" checksum="BAAB7894124587B3" modified="2005-09-27" version="1">MIVAIIVGAVSVVGAATALISIISSSDEDKDSTKALEQEKKSEDTSKSDRGKASGVQVSPDKVVEDNKPVETSQDLGATGITPGDNSDKQDALQARYLREGAKPKVHSSKSAKSKVTKPTKKPAVKFYDWSKKEYVSAATTFVQSVQQPSKVVESTSDVVPPKAPKVIIQPSSSEVREEGDKDRSKYIEPEKLIVQQILTPDVSLQLGKEDKVLSDPNYDPEVTVVELLKQMSLDKLSTAAQGKQKVFDETKEKKHKSEGKLSSVPVVHQEEMLTSDVDLQLSEDEKMLNDPNYDPEAAIIELLRQMNVEKLGATAQGEQEVSDTPEKYRYEGYSELSSVKVSSLSPAELIACSGKQFNNFMGSTCIRLLQKRYSSSDKRFKMFLQKFRSMQFIYCCEILQQLFYVQCNRLYVTDNTEVLLRNLQNPTLCVLVLKLCFALYCTIPLARQGFCDTVVYNTRICGKNLLDMLLVKVYNGVQHEGFKFRPIRQILYCQYLNKLSFSNVVLHDGDFFLGIYSLLCELSYLRVNSILRSHDILDFAIVGCAIMCGSMYDNYGFLINNKTSIDEEHNIMYLVEHMGSTGPQRGAFGQMCYDLLCQVRYMYNAGNNQLTKLHNMLFPRALIEQCSPGFRERAEEHVAGGTMSFNLIITDIASRICRMMDRYHLGPFEYEVSSYARLYDDIMRGLDKGTGNQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT45</accession>
    <name>Q3YT45_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="1">4.1.2.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.2.13" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304188.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0059"/>
    <dbReference type="EnsemblBacteria" id="AAZ68110">
        <property type="protein sequence ID" value="AAZ68110"/>
        <property type="gene ID" value="Ecaj_0059"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0059"/>
    <dbReference type="eggNOG" id="COG1830">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057069_0_0_5"/>
    <dbReference type="OMA" id="FVKVNYP"/>
    <dbReference type="OrthoDB" id="1560751at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="301" mass="32636" checksum="AB620D329A2835A0" modified="2005-09-27" version="1">MLANNPIQDILGFYESENPGVKANLTRMLLHGKVAGSGKLLILPVDQGMEHGPTKSFAKNYQSYDPHYHFQLALEGGFSAFAAPIGMLEAGASTYAGLIPLILKINSSILLSPKNSFPDQVVTSSVKDALRLGCSAIGLTIYPGSHNFFSMIRETRDLIAEAKAAGLAVVIWSYPRGNDISKQGETAVDIVAYAAHIAASLGANIIKVKLPTSHIERDSIPYDISDLKQRVEYVKLSCFSGRRLVVFSGGEAKTDEELLQEIQSIKSGKGDGSIIGRNSFQRSKNNAILMLEQIAKIYNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT58</accession>
    <name>Q3YT58_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ornithine carbamoyltransferase</fullName>
            <ecNumber evidence="5">2.1.3.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0046</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304175.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0046"/>
    <dbReference type="EnsemblBacteria" id="AAZ68097">
        <property type="protein sequence ID" value="AAZ68097"/>
        <property type="gene ID" value="Ecaj_0046"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0046"/>
    <dbReference type="eggNOG" id="COG0078">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_5"/>
    <dbReference type="OMA" id="DGNNVCN"/>
    <dbReference type="OrthoDB" id="988597at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="4">
        <location>
            <begin position="8"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="3">
        <location>
            <begin position="152"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="303" mass="34588" checksum="56DE114D688F9FF1" modified="2005-09-27" version="1">MNKQNIHSFIDLNLHTKEELNTILNYGAIIKKNPNQYSKHLSNKNIALIFLQPSTRTRISFEVGVNQLGGNAITLNGKDTQLIRGECIHDTAKVLSRYVDLIVIRILEHDSLLELNKYSTVPVINSLTQHSHPCQVLADIMTYEEKMGPIENATVSWIGDFNNMLVSWIHAATVLNFKLNISTLEYSENLDLLLKLVNEKGANVVYNKNPYDIIPDADIITTDSWVSMGFENKIKTEFFNYQVNAKLLEKAKPNYMFLHCLPAHRGEEVTSEIIDGLNSYIFDEAENRLHIQKAIMLWCLNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT70</accession>
    <name>Q3YT70_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-dependent DNA helicase RecG</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0034</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304163.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0034"/>
    <dbReference type="EnsemblBacteria" id="AAZ68085">
        <property type="protein sequence ID" value="AAZ68085"/>
        <property type="gene ID" value="Ecaj_0034"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0034"/>
    <dbReference type="eggNOG" id="COG1200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005122_7_1_5"/>
    <dbReference type="OMA" id="DNGFQAC"/>
    <dbReference type="OrthoDB" id="234717at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045562">
        <property type="entry name" value="RecG_dom3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033454">
        <property type="entry name" value="RecG_wedge"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19833">
        <property type="entry name" value="RecG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17191">
        <property type="entry name" value="RecG_wedge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="274"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="449"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="678" mass="76504" checksum="A9C794AC789DAAFE" modified="2005-09-27" version="1">MHDLSIFSSIYMLPGVNKVVGNLLKKLCGGDKIIDLLFHIPQNYIDRRTGLSEEAVGKIVTFIGTVKSHGFIGGRRKAQYKIVLDTCIGEVSLVFFHYSVKYLKSVLKVGSECVISGTLIRFFECLQITHPDYIITDVGKFHDISIIEPVYPLIKGVTSKRISKLVKLSIGLLPDFPEWIDERLLRDNQWDSWKESLIKIHHPKTLETVSLHKMRLAYDELLSHQISMKMVRKFDCKQGVSIISKRIYHDDILNKLPFKLTAGQEEVISQITESQALNSRMVKLLIGDVGSGKTVVALFAILNVIENEGQVAFMAPTEILAEQHYRWIQGILSGIPVSVELLTSKVRKKQDIKRKLQLGECKVVVGTHALFQDGVDFNNLNLIIIDEQQRFGVLQRMKLINKSNLADVLFMTATPIPRTLEQVVYGDIDCLRLEDKPHNRLPIHTSIINIEKLYEVVTKLQFALRDGNKAYWICPYIEDSELVDIAAAEKRFFSLKEVFGQEVGLVHSRLSKVERDDVMMSFYHGTIKLLVATTVIEVGVDVPDATIIIIENAEQFGLSQLHQLRGRVGRSDKASFCVLLHGSMVSKVAYKKLCILRKSQDGFYIAEQDLLLRGSGDVLGVKQSGLSNFKFADIYSDQNLISIATEQAQEILNANTKLNDNLAQLLCMFGYDVSAMNY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT96</accession>
    <name>RUVC_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Crossover junction endodeoxyribonuclease RuvC</fullName>
            <ecNumber evidence="1">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Holliday junction nuclease RuvC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Holliday junction resolvase RuvC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvC</name>
        <name type="ordered locus">Ecaj_0008</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvC family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304137.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT96"/>
    <dbReference type="SMR" id="Q3YT96"/>
    <dbReference type="STRING" id="269484.Ecaj_0008"/>
    <dbReference type="EnsemblBacteria" id="AAZ68059">
        <property type="protein sequence ID" value="AAZ68059"/>
        <property type="gene ID" value="Ecaj_0008"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0008"/>
    <dbReference type="eggNOG" id="COG0817">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091257_1_0_5"/>
    <dbReference type="OMA" id="AICHIWR"/>
    <dbReference type="OrthoDB" id="1815080at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16962">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00034">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020563">
        <property type="entry name" value="X-over_junc_endoDNase_Mg_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002176">
        <property type="entry name" value="X-over_junc_endoDNase_RuvC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30194">
        <property type="entry name" value="PTHR30194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02075">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00696">
        <property type="entry name" value="RSOLVASERUVC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00228">
        <property type="entry name" value="ruvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01321">
        <property type="entry name" value="RUVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225141" description="Crossover junction endodeoxyribonuclease RuvC">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00034"/>
        </source>
    </evidence>
    <sequence length="156" mass="17426" checksum="41635EAFEA2034F6" modified="2005-09-27" version="1">MNVIGLDPGLNHTGWGILSIEKDIKLIGNGVIKTNNKETPGQRLNKIHKELINILNSYQINSASMEEVFINKNPRSSISLCYARGVLLLTLNAMNIQVFEYSSNYVKKSITGNGHAKKEQVHFMVEKILNVEFKGTYDISDAIAVALCHAYSKNNF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT97</accession>
    <name>PLSY_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-3-phosphate acyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acyl-PO4 G3P acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Acyl-phosphate--glycerol-3-phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">G3P acyltransferase</fullName>
            <shortName evidence="1">GPAT</shortName>
            <ecNumber evidence="1">2.3.1.275</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lysophosphatidic acid synthase</fullName>
            <shortName evidence="1">LPA synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">plsY</name>
        <name type="ordered locus">Ecaj_0007</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an acyl phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:34075"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="2.3.1.275"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Probably interacts with PlsX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PlsY family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.275" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304136.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT97"/>
    <dbReference type="SMR" id="Q3YT97"/>
    <dbReference type="STRING" id="269484.Ecaj_0007"/>
    <dbReference type="EnsemblBacteria" id="AAZ68058">
        <property type="protein sequence ID" value="AAZ68058"/>
        <property type="gene ID" value="Ecaj_0007"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0007"/>
    <dbReference type="eggNOG" id="COG0344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081254_4_0_5"/>
    <dbReference type="OMA" id="WRHRGNL"/>
    <dbReference type="OrthoDB" id="1691856at2"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043772">
        <property type="term" value="F:acyl-phosphate glycerol-3-phosphate acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01043">
        <property type="entry name" value="PlsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003811">
        <property type="entry name" value="G3P_acylTferase_PlsY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30309">
        <property type="entry name" value="PTHR30309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02660">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01207">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00023">
        <property type="entry name" value="TIGR00023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000188363" description="Glycerol-3-phosphate acyltransferase">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01043"/>
        </source>
    </evidence>
    <sequence length="191" mass="21306" checksum="C6510BE777B742B2" modified="2005-09-27" version="1">MNIYTSILVLSYLIGSIPFGLILSYIGGLGDIRKIGSGNIGATNVFRKSKKLAVVTLILDSLKGFVSVMLAKNFSSDQTFVFMSALFSIIGHMFPVWLSFKGGKGVATLLGSIMFIEYKFVIYFTIFWIIVFVIFRYSSLSSIISTISIMLLVYTHYSANESITFLVMSLLVIVQHIENIVRIIKGKENKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQP0</accession>
    <name>SYT_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="1">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thrS</name>
        <name type="ordered locus">Ecaj_0934</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-threonyl-tRNA(Thr)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305038.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YQP0"/>
    <dbReference type="SMR" id="Q3YQP0"/>
    <dbReference type="STRING" id="269484.Ecaj_0934"/>
    <dbReference type="EnsemblBacteria" id="AAZ68965">
        <property type="protein sequence ID" value="AAZ68965"/>
        <property type="gene ID" value="Ecaj_0934"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0934"/>
    <dbReference type="eggNOG" id="COG0441">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008554_0_1_5"/>
    <dbReference type="OMA" id="FYYDFAY"/>
    <dbReference type="OrthoDB" id="900765at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="PTHR11451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000020386" description="Threonine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Catalytic" evidence="1">
        <location>
            <begin position="242"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01228"/>
        </source>
    </evidence>
    <sequence length="633" mass="73262" checksum="283BD6EE82933D34" modified="2005-09-27" version="1">MINIYFNNNLCKQFHRGIKGYDVVADLFPELKNKAIAVKVNGESYDLSREITENCTFEVITTNSEEGLEIIRHDTAHIMAQAVKEIFPDTQITIGPTIKDGFYYDFATEHNFSSNDLEIIEKKMIEIINKNESFIREVWTREEAIKFFSSIGEDYKVKIISKIPNNENITVYKQGNFIDLCRGPHAPSTKTSKSFKLTKVSGSYWQGNSSNEKLQRIYGTAWRNEEELKSYLNNLIEIEKRDHRKIGKELELFHIQNEACGQVFWHKKGWTIYRTIENYIRKKLETNGYIEVKTPMLLNKELWEKSGHWDKFRENMFLSEAEDKVLAIKPMNCPCHIQIFNSKIRSYRDLPIRMAEFGICHRYEASGALHGLMRVRGFTQDDAHIFCTESQVISEALKFCNLLMEVYKDFGFTNILVKFSDRPENRAGSDETWDRAESALKKSVEAANLNYVLNPGDGAFYGPKLEFVLKDAIGREWQCGTLQMDFVLPERLGAYYVGSDGKKHHPIMLHRAILGTFERFIGILIEHHSGKFPIWLAPTQLSILTISEESIEYANSLKTKAEEHNIRVELDATNEKINYKIRNHIHKKIPVFWIVGKKEVNENSVSIRYLESNTQQVMPIDKALKTLLTCVSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQP4</accession>
    <name>RIMM_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome maturation factor RimM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimM</name>
        <name type="ordered locus">Ecaj_0930</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Binds ribosomal protein S19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The PRC barrel domain binds ribosomal protein S19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RimM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305034.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YQP4"/>
    <dbReference type="SMR" id="Q3YQP4"/>
    <dbReference type="STRING" id="269484.Ecaj_0930"/>
    <dbReference type="PRIDE" id="Q3YQP4"/>
    <dbReference type="EnsemblBacteria" id="AAZ68961">
        <property type="protein sequence ID" value="AAZ68961"/>
        <property type="gene ID" value="Ecaj_0930"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0930"/>
    <dbReference type="eggNOG" id="COG0806">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077636_0_1_5"/>
    <dbReference type="OMA" id="DEFYHAD"/>
    <dbReference type="OrthoDB" id="1497415at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00014">
        <property type="entry name" value="Ribosome_mat_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011961">
        <property type="entry name" value="16S_RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002676">
        <property type="entry name" value="RimM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036976">
        <property type="entry name" value="RimM_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01782">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02273">
        <property type="entry name" value="16S_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000244127" description="Ribosome maturation factor RimM">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="PRC barrel" evidence="1">
        <location>
            <begin position="93"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00014"/>
        </source>
    </evidence>
    <sequence length="173" mass="19543" checksum="437D7243B6518AB5" modified="2005-09-27" version="1">MRNDLICLGVITSSHGISGHVKIRTFTESPEDFTSYGMLTDGTNTYKISIVSVISQNIIIAKIKNIVSRNDADLLRNKKLFIEKDKLPNLTNNDEFYHNDLIGLNVILENNKVYGNIKKIHNFGSCDIIEICLSNSKKSTMLPFTKNIFPYINIKEKYIIINLPETIGNSSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQQ6</accession>
    <name>Q3YQQ6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Major outer membrane protein P30-20bacterialsurface antigen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0918</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305022.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68949">
        <property type="protein sequence ID" value="AAZ68949"/>
        <property type="gene ID" value="Ecaj_0918"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0918"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="GGYYHKV"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="275" mass="30872" checksum="7A8FD6AB6E6A788A" modified="2005-09-27" version="1">MVIKMNYKRFVVGVTLSTFVFFLSDGAFSDANFSEGRRGLYIGSQYKVGIPNFSNFSAEETIPGITKKIFALGLDKSEINTHSNFTRSYDPTYASSFAGFSGIIGYYVNDFRVEFEGSYENFEPERQWYPENSQSYKFFALSRNATNSDNKFIVLENNGVVDKSLNVNVCYDIASGSIPLAPYMCAGVGADYIKFLGISLPKFSYQVKFGVNYPLNVNTMLFGGGYYHKVVGDRYERVEIAYHPTALSDVPRTTSASATLNTDYFGWEIGFRFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQR3</accession>
    <name>Q3YQR3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0831</name>
        <name evidence="3" type="ordered locus">Ecaj_0911</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2005-07" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Ehrlichia canis str. Jake.</title>
            <authorList>
                <person name="Palenik B."/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Mavrommatis K."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304939.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0831"/>
    <dbReference type="EnsemblBacteria" id="AAZ68862">
        <property type="protein sequence ID" value="AAZ68862"/>
        <property type="gene ID" value="Ecaj_0831"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68942">
        <property type="protein sequence ID" value="AAZ68942"/>
        <property type="gene ID" value="Ecaj_0911"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0831"/>
    <dbReference type="KEGG" id="ecn:Ecaj_0911"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="MHYKKFF"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014309228" evidence="1">
        <location>
            <begin position="26"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="280" mass="30962" checksum="D1BB28B5AF0E6CA3" modified="2005-09-27" version="1">MNYKKILVRSALISLMSILPYQSFADPVGSRTNDNKEGFYISAKYNPSISHFRKFSAEETPINGTNSLTKKVFGLKKDGDITKKDDFTRVAPGIDFQNNLISGFSGSIGYSMDGPRIELEAAYQQFNPKNTDNNDTDNGEYYKHFALSRKDAMEDQQYVVLKNDGITFMSLMVNTCYDITAEGVSFVPYACAGIGADLITIFKDLNLKFAYQGKIGISYPITPEVSAFIGGYYHGVIGNKFEKIPVITPVVLNDAPQTTSASVTLDVGYFGGEIGMRFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQS1</accession>
    <name>Q3YQS1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0903</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0903"/>
    <dbReference type="EnsemblBacteria" id="AAZ68934">
        <property type="protein sequence ID" value="AAZ68934"/>
        <property type="gene ID" value="Ecaj_0903"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0903"/>
    <dbReference type="HOGENOM" id="CLU_1459155_0_0_5"/>
    <dbReference type="OrthoDB" id="2124900at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="185" mass="21968" checksum="941062FE3115D72F" modified="2005-09-27" version="1">MATYMIHKAKIIEPMFSPSILTDYIILSILNNVQDHNSFIICITKLYKLILSSITKKLINVKDNIAVLHNINLYTLYNKCSKFKSTQSFYEMYFYTKNIKDRILNFNSLYFIKYNVTQKNSNESYYKSTYKSHNFFNATYTTSFIHQHKLYTKYSITNQHNKSTKSFSNKILAKNILNNQENSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQS4</accession>
    <name>Q3YQS4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305007.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0900"/>
    <dbReference type="EnsemblBacteria" id="AAZ68931">
        <property type="protein sequence ID" value="AAZ68931"/>
        <property type="gene ID" value="Ecaj_0900"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0900"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="PYLCTGI"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="264" mass="30625" checksum="70B4E83DCEA9D910" modified="2005-09-27" version="1">MYNIINYVIKYTIALAFLLLPRVSFSILIGNIEKSIKLLSVHINSQYKPSISQISNYLIQENNSKEKKINILNLSNNTITYNMQLENSTTNFRFIIGYFFKRLRFAVEDSYEEFHIKDNDSLKANLSKYSYKMYNEDFQNFTIATDNKLSITSAIVNICYDILINNTTVLPHLCTAVGICSTGFFNDMRFKLLYQRKIGLGYLINSNVMLFFNVYYHKVMRNKLKNLLTQYSVDINAFLDAITVLANTDIGYFGSEVGVRFIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQT4</accession>
    <name>Q3YQT4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="4">1.1.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">IDP</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">NADP(+)-specific ICDH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Oxalosuccinate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Ecaj_0890</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304997.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0890"/>
    <dbReference type="EnsemblBacteria" id="AAZ68921">
        <property type="protein sequence ID" value="AAZ68921"/>
        <property type="gene ID" value="Ecaj_0890"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0890"/>
    <dbReference type="eggNOG" id="COG0473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_1_1_5"/>
    <dbReference type="OMA" id="TCAHKAN"/>
    <dbReference type="OrthoDB" id="1551125at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014273">
        <property type="entry name" value="Isocitrate_DH_aproteobac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040978">
        <property type="entry name" value="TT1725"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF43">
        <property type="entry name" value="PTHR11835:SF43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18324">
        <property type="entry name" value="TT1725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02924">
        <property type="entry name" value="ICDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Iso_dh" evidence="16">
        <location>
            <begin position="4"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAZ68921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="482" mass="53398" checksum="2F767FE4387A9A5C" modified="2005-09-27" version="1">MSIPITVAYGDGIGPEIMEAVLLILSEAESGLVIETIEVGHNLYKKEWSSGIAPSSWDSIFRTKVLLKSPTMTPQGQGHKSLNVTLRKKLGLYANIRPCISYHPVIKTKYPNLNIVIIRENEEDTYTGIEHRLTSDTYQCSKVITRSGSERICNYAFHYATVHNRKKVTCLIKDNIMKMTDGIFHKSFSKIAENYPSITSDHYIVDIGMAKVAANPENFDVIVTTNLYGDIVSDIVAELSGSIGLAGSANIGDNYAMFEAVHGSAPDIAGKNIANPSGLLNAAIQMLIYLKKFDKAELIYNALLKTLEDGMHTADIYQSQVSKGKVSTMGFAKAIIDNFGQSPTQFPKSIFQNSAGESGISYTYKPSYATKVLVGVDLSISCDSADLNLKQLISNIQGVTHDKLELVLIHSKGLEIWPDESSNANLSYMDQICCRFYMKGQSDKIVNEHVNQLLFDMSKKKIDVVKMEKLYLYDNQPGFFTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQV0</accession>
    <name>Q3YQV0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="15">H+-transporting two-sector ATPase, B/B' subunit</fullName>
            <ecNumber evidence="15">3.6.3.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ecaj_0874</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0). The b'-subunit is a diverged and duplicated form of b found in plants and photosynthetic bacteria.</text>
    </comment>
    <comment type="function">
        <text evidence="11">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ATPase B chain family.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.14" evidence="15"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304981.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0874"/>
    <dbReference type="EnsemblBacteria" id="AAZ68905">
        <property type="protein sequence ID" value="AAZ68905"/>
        <property type="gene ID" value="Ecaj_0874"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0874"/>
    <dbReference type="eggNOG" id="COG0711">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079215_1_2_5"/>
    <dbReference type="OMA" id="NQIFWLV"/>
    <dbReference type="OrthoDB" id="1544382at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002146">
        <property type="entry name" value="ATP_synth_b/b'su_bac/chlpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00430">
        <property type="entry name" value="ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4 13" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAZ68905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="167" mass="19545" checksum="E527EC3FE696A937" modified="2005-09-27" version="1">MDIIPQLDITSYPSQLFWFFLSFGILYLVISKNILPKVENVIRKRYNITRGSINCVENDLNLIQHELKKQLLSLDEVKAEADKIISSALQEVKSTNADLITALNEEIKKMFSTADEYMHNLKHQVEQELIDLTCEIALLYYKKMLGTEYIDKDKLRDITIRLYKEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQV1</accession>
    <name>Q3YQV1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase F(0) sector subunit c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">F-type ATPase subunit c</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ecaj_0873</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_006011614.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0873"/>
    <dbReference type="EnsemblBacteria" id="AAZ68904">
        <property type="protein sequence ID" value="AAZ68904"/>
        <property type="gene ID" value="Ecaj_0873"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0873"/>
    <dbReference type="eggNOG" id="COG0636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148047_4_0_5"/>
    <dbReference type="OMA" id="CMGFCIL"/>
    <dbReference type="OrthoDB" id="2078221at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
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        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
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        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
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        <property type="entry name" value="F/V-ATP_Csub_sf"/>
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        <property type="entry name" value="PTHR10031"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPASEC"/>
    </dbReference>
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        <property type="entry name" value="SSF81333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="13">
        <location>
            <begin position="7"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAZ68904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="73" mass="7788" checksum="97EE6BB50D1F8648" modified="2005-09-27" version="1">MESLKFIAVGLSVFGMVASALGVANIFSTMLNGLARNPETEDKLKKYVYTGAALVEAMGLFSFLLALLLIFVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQW0</accession>
    <name>Q3YQW0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0864</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Skp family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
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    <dbReference type="STRING" id="269484.Ecaj_0864"/>
    <dbReference type="EnsemblBacteria" id="AAZ68895">
        <property type="protein sequence ID" value="AAZ68895"/>
        <property type="gene ID" value="Ecaj_0864"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0864"/>
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        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1479844_0_0_5"/>
    <dbReference type="OMA" id="NAMEMVY"/>
    <dbReference type="OrthoDB" id="1568657at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005632">
        <property type="entry name" value="Chaperone_Skp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024930">
        <property type="entry name" value="Skp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35089">
        <property type="entry name" value="PTHR35089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03938">
        <property type="entry name" value="OmpH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00935">
        <property type="entry name" value="OmpH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111384">
        <property type="entry name" value="SSF111384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230935" evidence="4">
        <location>
            <begin position="23"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="49"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="182" mass="20914" checksum="06E0426B9ADEB04B" modified="2005-09-27" version="1">MRLKLFLIIFILATTLSNYALAKNDQNIPVAFIDRDIIISEALSVKSIRAQLDDKRAALQQDFAAREEELHKAEEELSKQKTILSSEAFEKKVADFKTKVSNLQQDISVKGAELENMYMNAMEMVYNKIKNISTKIAKEKNINLILFLIKKNQVFYATDGIDFSNEVLERLNKELPNIEIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQW1</accession>
    <name>Q3YQW1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Outer membrane protein assembly factor BamA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">bamA</name>
        <name evidence="8" type="ordered locus">Ecaj_0863</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the Bam complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the BamA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304971.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0863"/>
    <dbReference type="EnsemblBacteria" id="AAZ68894">
        <property type="protein sequence ID" value="AAZ68894"/>
        <property type="gene ID" value="Ecaj_0863"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0863"/>
    <dbReference type="eggNOG" id="COG4775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007664_1_0_5"/>
    <dbReference type="OMA" id="IEWISPM"/>
    <dbReference type="OrthoDB" id="258122at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01430">
        <property type="entry name" value="OM_assembly_BamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010827">
        <property type="entry name" value="BamA/TamA_POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023707">
        <property type="entry name" value="OM_assembly_BamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="PTHR12815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF23">
        <property type="entry name" value="PTHR12815:SF23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07244">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006076">
        <property type="entry name" value="OM_assembly_OMP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03303">
        <property type="entry name" value="OM_YaeT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 6" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009019174" description="Outer membrane protein assembly factor BamA" evidence="6">
        <location>
            <begin position="19"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="7">
        <location>
            <begin position="23"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="7">
        <location>
            <begin position="173"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="7">
        <location>
            <begin position="347"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="769" mass="87226" checksum="1CEFD7C6F6EA0809" modified="2005-09-27" version="1" precursor="true">MKYIVIILILFSSSFAYAEALNTKVKQVQINGNHRLDYKTIYFYSKINLEDNVTQETIDQIIKNLHSTQLFSHIDVHVNKDNYLVINVKENPVVNNIIFHGNKEFNKKDLQNDILKLKKLAVFTKSKLQQDISNLLMLYQSKGKLGAKIAYEVKELENNKIDVIIKINEGPTSRIKNIKFIGNKFFSDAILKKAIQSSEHYIYKIFSSNTKFASERLMLDQAGLYNFYTSKGFIDFKVKSVVPELRDDHNINLIFSVEEGIRYKFGNSNIIIDKQVTNHQQLKEEIQKLVMSKSGDIFNREVINNSIEKITQHLSNNGNFFSDVKHEYNIKNDTVDIDYIIYTGNKVYINKINITNNKTTLDQVIRQKLNISEGDIYNASVINRSYKNILGSGFFESVNVENHKINDSLVDLTFQVKERGTGTFAVSAGFSSVTGLVGKINIQERNLFGTGKVLSLQAEKSTSSLSSSVDLVMPNFLDTDSAVGFGLFYAHQNKPGGKNYSTTKLIGNSDSAFSSSNAGFMLHTSHDITDDLGLSLNYAYKHVNIFNIKDTASDLIKEQGGKSIDSSIGYALQFRKFDNLSKIRDGYFIKLRQNFSGLGGTLHYIKTEGSFNYSRELFPKISNDILLSIKTSMGYVFSYKSDEQVKINQRFIIGSNEIRGFHVSGIGPRDKKTLDALGGKFYFSMINQVDFPIGLPDDLGIKGSLFIDAATLFGLDYTDKEYYENKSLRISAGFGFSWRSPFGPMRLDFGFPIRKEDYDVTDMIRFSMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQY6</accession>
    <name>Q3YQY6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0838</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68869">
        <property type="protein sequence ID" value="AAZ68869"/>
        <property type="gene ID" value="Ecaj_0838"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0838"/>
    <dbReference type="HOGENOM" id="CLU_002895_0_0_5"/>
    <dbReference type="OMA" id="RSMINHE"/>
    <dbReference type="OrthoDB" id="1226603at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1510" mass="171143" checksum="C56D1AEA2DC730D8" modified="2005-09-27" version="1">MFMGRWWCKNIVLYLAVLLFSFCPALGKSEYVTPEKLEMYFAMYNGKHFTSEEFIKGMGYHRVRFTDYVGFFYGYWFGIHFSDYNQVFDYKENDSVYIRFAGHNQIRTPSFNGYYNGWYKDIEDIYAAQLGINPDKNSRTVNEHDIMLMQLSSYWGKVTEHAIPIGSCKSIYKSKNPGFYRSLSARAFLTENGVHKMVCVCLINDCNPNTHCINGQVRCKRVKKSVDPPPFCSILDSANIVSITPLKFSQQTFFRPGVRIHIYSGSGKPYIEDLYAQSYKIGDKTSYDISYAGIPYKFQIYKAGYDMVCAEYLNNGVPEKKVCLPSPGLVRPKVTPSSGGVHIQYQDCQGLPSCDVDVSPGTKDSNMFFSVIKPKVNLDDYTLISQYECDGGQVVHDISKCSGKSAKDLGYASDSNGNVTCVVDMPFVRMKYFLKKNNRDLWLSRYEKMFLGYGVVNDETDDGKKSESYVMCDYGSAINIANMQQGELDKVKSVKQDLFFDIAGHYNPKGNPCVSNVLYKYDSTRFYKKDRGVSCKDNAASNNLMGEFDGCSSLYDSDDDFTNFFHEGDDELDKIKPLNPILQGMCVSNFPSYKYGKRSVVRNVLQNTYKLSIDKKNSTCDFLKIEAWGGGASGVSRSGKSGKPGNYVMGILKFDKDVVDKKLIIDIGTGGTGSSHLSNAGGDTTVKLCDDDDENCTIKLVANGGDVGGNYLKDNSEGVDKLVHYRFATGLRDSGEGEILIPYQSPDEPYGKLQKDSKECNCNSSTLEKNSNKYFGSGGCSGIYNCAQEGADGMVKLTCEKWSGSVGKIKLIDENSCNNVLTAFIEKTNKPMGGMPDKVKKFLDKISDVTFCRKVQNMTSLITALYDYFSMINTLFSSKNMSNDLPNLRKRLLTELSNEKVQLVFKELGIDDNPETILLYIDAINFNYGISISKPPEGLLNHYVADDKFDYDLSKHDVDYNTLSDDEAMMFNVVGEDTERWFSVKLQNQQFVQEYGEFIDFIHKNVMADKNGYKKNNIVVSWMYNFFETDKQIFDLYAAPFVELMLGVDLDKFMKWRQCGDTNVKLFDAIALYKDKLPTGVQEFIEKISNPNFCVEFPGMTLLGEYISELENYMSGNLFNSLNKNDIIDRGSDKLSNVVEQLDDSVGENYEVFHNMGILEDETLLIDAVVFNYVMSKLNISSAQIDNVFSLLNNVSVRYTDRLPYDTILELLRNANSMKYGQDGVFRANMIALWSYTSYYVFDVRWSVNYFRSFVKLLLGIDLKSIKLRRCDQDLVDLFKAFRVTEYSLSKTLQDFLIDTNNKDFCQKMLQFPELGRVLSEYKNILRDILHSGDIFNDPSSATVNMQLSNIMKLRSRIKYLLNDPAVYSVFTDVGITNTQAQIPLIIDAVMLNTLMLDANISSANLSKLISFAVKDRTLSVVRVGDPVRKGNKILHRLRITKTVTKFPDDTILKLQRNANSTQYGNYGSYKTDIIVMWSCMLYLASKASWSSEEFESFVNLVLEIDVKNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR04</accession>
    <name>ARGC_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyl-gamma-glutamyl-phosphate reductase</fullName>
            <shortName evidence="1">AGPR</shortName>
            <ecNumber evidence="1">1.2.1.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetyl-glutamate semialdehyde dehydrogenase</fullName>
            <shortName evidence="1">NAGSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argC</name>
        <name type="ordered locus">Ecaj_0820</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + N-acetyl-L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.38" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304928.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YR04"/>
    <dbReference type="SMR" id="Q3YR04"/>
    <dbReference type="STRING" id="269484.Ecaj_0820"/>
    <dbReference type="EnsemblBacteria" id="AAZ68851">
        <property type="protein sequence ID" value="AAZ68851"/>
        <property type="gene ID" value="Ecaj_0820"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0820"/>
    <dbReference type="eggNOG" id="COG0002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006384_0_1_5"/>
    <dbReference type="OMA" id="PHLTPMI"/>
    <dbReference type="OrthoDB" id="951261at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000010993" description="N-acetyl-gamma-glutamyl-phosphate reductase">
        <location>
            <begin position="1"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00150"/>
        </source>
    </evidence>
    <sequence length="346" mass="38849" checksum="9B15964BFBC778D6" modified="2005-09-27" version="1">MSYQVSVAVVGATGYVGVELVRLLLAHPMVKIRYLCATQSTGKLLSSSYFHISQNYISISVSSFDDIDLDKLDVVFLCLPHGASSEIVKKIHNVVKVIDLSADFRIKDPDIYKQWYGVHYCPDLIKDFVYGLTEIYYEDIQKSSFIACPGCYPTSVLIPLFPLLRLRLVKSNNIIVDAKSGVSGAGRSVKQDNLFCEVYDAIKSYKVSNHRHIPEIEQELCFAACREDINLQFVPNLIPVKRGMMSNIYLELEVGVSLTDVREALLLFYRDSFFVVVDEEKAITTRSVVGTNYCYLGIFPGRLPNTIVIISVIDNLLKGAAGQAVQNFNVMMSYEEKLALSNIPYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR08</accession>
    <name>Q3YR08_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3'-5' exonuclease</fullName>
            <ecNumber evidence="3">3.1.26.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0816</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.26.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0816"/>
    <dbReference type="EnsemblBacteria" id="AAZ68847">
        <property type="protein sequence ID" value="AAZ68847"/>
        <property type="gene ID" value="Ecaj_0816"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0816"/>
    <dbReference type="eggNOG" id="COG0349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086957_0_0_5"/>
    <dbReference type="OMA" id="MGLNPHR"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="2">
        <location>
            <begin position="14"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="216" mass="24962" checksum="FCBD71248DCF7CB1" modified="2005-09-27" version="1">MIYLFYRWRKFYVPVFVHDCDLPNGLNFEDAVAIDTETMGLVCRRDRLCLVQLADSSGNVHLVKFSGNYQAPNLVSVLSDPNIIKIFHFARFDIAVMRYYLGMWANPCYCTKIASKLVRTYTDHHGLKELCYELLGVKLNKMQQSSDWGRESLTSEQLNYAASDVIYLHALKSKLDAMLQRENKRELAAACFKFLQTRAQLDLLGWENVDIFSHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR16</accession>
    <name>Q3YR16_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Aspartokinase</fullName>
            <ecNumber evidence="6 15">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">Ecaj_0808</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="6 15"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304916.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0808"/>
    <dbReference type="EnsemblBacteria" id="AAZ68839">
        <property type="protein sequence ID" value="AAZ68839"/>
        <property type="gene ID" value="Ecaj_0808"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0808"/>
    <dbReference type="eggNOG" id="COG0527">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_3_2_5"/>
    <dbReference type="OMA" id="DMIVQTI"/>
    <dbReference type="OrthoDB" id="1067792at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04261">
        <property type="entry name" value="AAK_AKii-LysC-BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041740">
        <property type="entry name" value="AKii-LysC-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="17">
        <location>
            <begin position="268"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="8"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="177"/>
            <end position="178"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="213"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000726-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AAZ68839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="417" mass="45726" checksum="4D1BA44FA1E86B4E" modified="2005-09-27" version="1">MERILVKKFGGTSLQDIKCINKVVEIIKQDINNNYKVVVIVSAMGKFTDNIISQIKQISDVKSPSERSEYDLIISSGEQISCGLLSLALQKVGINAQSWLGWQLPIVTTEDHTKARIIDINVNSLQDSLANNDVAIVAGFQGMHKNNRVTTLGRGGSDTSAVAIAVALGVDLCYIYTDVDGIYTADPNLVPKAHKLDYITYDEMIEMSSLGAKVLQVRSVEIAMKYNIKLCILSTFNPGKGTMLCKKGESHMESQLITGVTCNNKTASITLKQVKTLSGITTVFNAIAEKNINVDMIIQNMNDNNANDITFTISEEDLPTTKKFLTEIQSELMYQDLIINSEVAKVSIIGVGMISHSGIAYKMFDTLTSNNIKILAVTTSEIKISVLIYRKDSQLATVALHSAFELDSTESNLYTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR32</accession>
    <name>Q3YR32_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Auxin Efflux Carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0792</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the auxin efflux carrier (TC 2.A.69) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304900.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0792"/>
    <dbReference type="EnsemblBacteria" id="AAZ68823">
        <property type="protein sequence ID" value="AAZ68823"/>
        <property type="gene ID" value="Ecaj_0792"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0792"/>
    <dbReference type="eggNOG" id="COG0679">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056175_4_2_5"/>
    <dbReference type="OMA" id="CCIMSAV"/>
    <dbReference type="OrthoDB" id="2033473at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="305" mass="34134" checksum="EE5B369AB19DE713" modified="2005-09-27" version="1">MFFILLFKILPLYITVFLGFIAGKFLKIDSPSIARLLFYIMGPIVVFSGISQITINSEILSLPFFTWLVCCIMSAVVYYTSSFIFRDSTRNILAFSSGSSSMGFFGLPVALAMFDNHTVSIYLFCYVGMLMFENSFGFYIAAQGSYTPRKCIIKLTTLPAAYAAIVALTVNYFKIPVPPFISDVAINIRGAYVSLGMMLLGIAVANIKIFTIDWKFIFITLIIKYVFWPVLILGFIILDKLTIQLYNNDTYRALILLAIIPMSGTGIILATMLNNNPDKTAIMLLVNTTIGLFYVPLMISILLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR34</accession>
    <name>Q3YR34_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exodeoxyribonuclease VII small subunit</fullName>
            <ecNumber evidence="3">3.1.11.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the XseB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.6" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304898.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0790"/>
    <dbReference type="EnsemblBacteria" id="AAZ68821">
        <property type="protein sequence ID" value="AAZ68821"/>
        <property type="gene ID" value="Ecaj_0790"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0790"/>
    <dbReference type="eggNOG" id="COG1722">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145918_3_1_5"/>
    <dbReference type="OMA" id="DDEYTFE"/>
    <dbReference type="OrthoDB" id="2057189at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003761">
        <property type="entry name" value="Exonuc_VII_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037004">
        <property type="entry name" value="Exonuc_VII_ssu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02609">
        <property type="entry name" value="Exonuc_VII_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116842">
        <property type="entry name" value="SSF116842"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01280">
        <property type="entry name" value="xseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="62" mass="7263" checksum="0173CDC6ED2BF721" modified="2005-09-27" version="1">MSIDDEYTFESAIEQLELIVKELESNQVSLARSIELYSLGKKLYEYCSKTIEEIELKIEVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR39</accession>
    <name>Q3YR39_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304893.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0785"/>
    <dbReference type="ESTHER" id="ehrcj-q3yr39">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68816">
        <property type="protein sequence ID" value="AAZ68816"/>
        <property type="gene ID" value="Ecaj_0785"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0785"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_1_0_5"/>
    <dbReference type="OMA" id="RNARDFE"/>
    <dbReference type="OrthoDB" id="1498437at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="30"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="283" mass="32537" checksum="0D3735820FB63BF1" modified="2005-09-27" version="1">MISYTLNISQLKEKFTMCYYYFNSTSNNYLICVHGITRNARDFDYLANTLSSNYKIICPDIVGRGKSSWLQDYQLYTYSTYCKSIVYLLRHLKIDKVDFLGTSMGGIIGMYLAAYFPNLINKLIINDIGPSIRVSTLKKISQHININPTFNTIDEAVVYIKNLLINFGINQENHWQHIITHSIIQNPNNTYSLASDPKIGMALDEDISKIESDTWNIWNIWDKIQSKILVIHGSLSGVLTHSTLKKMLLSKQNIDSIEYRNIGHAPALMDDYQIQDIKNWLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR40</accession>
    <name>Q3YR40_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 14">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="7">IMP dehydrogenase</shortName>
            <shortName evidence="7">IMPD</shortName>
            <shortName evidence="7">IMPDH</shortName>
            <ecNumber evidence="7 14">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">guaB</name>
        <name evidence="16" type="ordered locus">Ecaj_0784</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>H2O + IMP + NAD(+) = H(+) + NADH + XMP</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="7">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 14">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 13">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="7 14"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304892.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0784"/>
    <dbReference type="EnsemblBacteria" id="AAZ68815">
        <property type="protein sequence ID" value="AAZ68815"/>
        <property type="gene ID" value="Ecaj_0784"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0784"/>
    <dbReference type="eggNOG" id="COG0516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022552_2_2_5"/>
    <dbReference type="OMA" id="MGYCGAK"/>
    <dbReference type="OrthoDB" id="532857at2"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="PTHR11911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3 7 14" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10 14" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 7 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="15">
        <location>
            <begin position="89"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="15">
        <location>
            <begin position="152"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="7 9">
        <location>
            <begin position="337"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="7 9">
        <location>
            <begin position="360"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="7 9">
        <location>
            <begin position="384"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="7 8">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="247"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <begin position="297"/>
            <end position="299"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAZ68815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="485" mass="52049" checksum="6CA7273CAE1EC605" modified="2005-09-27" version="1">MELSYAFDDILIIPSASNVLPSETDVTTYITNEIELKIPLISAAMDTVTEAKLAIALAQHGGIGCIHKNLPIDKQLLEVRKVKKYESWIVYNPIAVSPDDSLAVALSIMEEYSYSGIPVVTDTENGKLLVGILTNRDVRFIEDKGCKVADIMTKDHLITVPEGIERADAIKLLHQYRKERLIVVDNNYCCVGLITVKDIEKFNQFPNSCKDSGARLRVAAAVGTGTKDGIERAEALIAEDVDIIVVDTAHGHSERVLTTVKEIRSLFPHAQIIGGNVATAEGAQALIEAGVDSVKVGIGPGSICTTRIVTGVGVPQFSAILNVANACKNKKIKVIADGGIKYSGDIAKSIAAGADVVMIGSIFAGTDESPGEIIIYNGRAYKSYRGMGSVGAMKHGSASRYFQENNHKFIPEGIEGRVPLKGPVAGVIHQLVGGLKSAMGYTGNRNISDMKTKCKFTSITSAGLRESHVHDVIITREASNYDPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR52</accession>
    <name>Q3YR52_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304880.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68803">
        <property type="protein sequence ID" value="AAZ68803"/>
        <property type="gene ID" value="Ecaj_0772"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0772"/>
    <dbReference type="HOGENOM" id="CLU_1624539_0_0_5"/>
    <dbReference type="OrthoDB" id="2140837at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="163" mass="18295" checksum="D8F1BD81F1645CD0" modified="2005-09-27" version="1">MFSDLKSSAFSIVNSTSNFSLDLGDEFFNNTVANQTDLIIHDANGDKVIGDNIIAISIIVAGIFIVVAVGICMFWLNRNESVLRERYMRCAQNPDTLKMDQPSTSGVTFCNAVSDEVELQQMDDVELRQRKVEETHFPKTLMSSPSNNSNNRECTMYYPSNFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR53</accession>
    <name>Q3YR53_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0771</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304879.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68802">
        <property type="protein sequence ID" value="AAZ68802"/>
        <property type="gene ID" value="Ecaj_0771"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0771"/>
    <dbReference type="HOGENOM" id="CLU_1132201_0_0_5"/>
    <dbReference type="OMA" id="ERSICCN"/>
    <dbReference type="OrthoDB" id="2153575at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="245" mass="27180" checksum="10C05578BDBCF6F4" modified="2005-09-27" version="1">MSFLDVISGVFASLTSNFFNNTTNSTLGTIRGNVTGVYNTLVNYVTTFFNSTVVNTNAVTGSSALNNTLPTIDNDTIHFVNSTIDDVLRNTNHSVINTSSSSDDWNKFFYCLLALGFVCVIRRICYHFMRGFREERHRDLMIRGFFSEAEVENNGIGCESRERSICCNVKENIVRQRNDISTEGDEGLSVNQCEEELHSICGGARTDISEKGSDSSSIGCEEIALNTMSTNVVVEMKVTEMSTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR66</accession>
    <name>Q3YR66_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidate cytidylyl transferase</fullName>
            <ecNumber evidence="2">2.7.7.41</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.41" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0757"/>
    <dbReference type="EnsemblBacteria" id="AAZ68789">
        <property type="protein sequence ID" value="AAZ68789"/>
        <property type="gene ID" value="Ecaj_0757"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0757"/>
    <dbReference type="eggNOG" id="COG4589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037294_1_1_5"/>
    <dbReference type="OMA" id="LIMLYFC"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="216" mass="24135" checksum="C14415F8ADFB77DA" modified="2005-09-27" version="1">MVSNNFFMRSFSSVLIFCIVFLSLYFGRMTFYCLCFFLAVVSSVELFNIMKHKSLYYIPSIFIVVIPYASLVYIYNLLHGEIILIWLIAVVWGTDIAAYFTGKSIGGKKMIPVISPNKTWAGLLGGIAAGTFISVVISIIFGIFFVFHAFVGGIIIAFVAQVGDVAESCIKRFYKVKNSGTFIPGHGGILDRMDSFIFTAPLVAYYVQKFSKFFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR72</accession>
    <name>Q3YR72_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0751</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68783">
        <property type="protein sequence ID" value="AAZ68783"/>
        <property type="gene ID" value="Ecaj_0751"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0751"/>
    <dbReference type="HOGENOM" id="CLU_1145775_0_0_5"/>
    <dbReference type="OMA" id="FENAMIC"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="242" mass="28108" checksum="09379A85727A4950" modified="2005-09-27" version="1">MYFIQSKSWIMECVPVGIIITIFTIFMLIMLAFIVFLFIDLIYFNIDLMKIYKSVESEVKDQLNYELESADISNDKDLLQVTNVIEYIIDQLTILHDACLDTNRSQSFENAMICLLNARVQAHMAEMYQNGETLSNEDEMYILAELKAREVKNGNQLYEKKDLRRGIIRAIKDAISYTENGVRICAFMLYDATEQKLILCRMKAAFDVCKAGIKSIEKKETEQNVIDDIQLYHEAYEHCVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR77</accession>
    <name>Q3YR77_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304855.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68778">
        <property type="protein sequence ID" value="AAZ68778"/>
        <property type="gene ID" value="Ecaj_0746"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0746"/>
    <dbReference type="HOGENOM" id="CLU_1141180_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="243" mass="27010" checksum="D712CC96770EEE46" modified="2005-09-27" version="1">MDNYCIIICAALVILLAILMAVLFGLLYKSTRDLKRSLESQSKSYNSEIGLLKQSLESLKANIAGLNLQNINSVLNRVNTYDKKLEIIKNDIKGFSSKCNSLETACDTLKKQLISAHGELASLSKKIHEAWKDKNKDGTVAIPSQNQIFDIDLMALVEELNIMKMAVAKLMSQNETHDLMNLEHSVSNLVCSVSSLSDSLSALESERVEYSIYIKNIAAKVASNLDEITFRVSAIERKISDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR89</accession>
    <name>Q3YR89_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68766">
        <property type="protein sequence ID" value="AAZ68766"/>
        <property type="gene ID" value="Ecaj_0734"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0734"/>
    <dbReference type="HOGENOM" id="CLU_1913760_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="132" mass="15146" checksum="144A331F4C50464E" modified="2005-09-27" version="1">MLQKKCDDLLEDVKSKALSVKIFREEVVHVRARLVKIVELMYQEFSTRLTVLSRSLISTVTDGYPIIRRKMQRDHSLTPQAMQQGVGALCRAFVVNIDKELEKTKKACHIKCKELQIEVEEVLGSDGILKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR95</accession>
    <name>Q3YR95_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304837.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68760">
        <property type="protein sequence ID" value="AAZ68760"/>
        <property type="gene ID" value="Ecaj_0728"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0728"/>
    <dbReference type="HOGENOM" id="CLU_1213267_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="228" mass="25733" checksum="D2D6667301B4C487" modified="2005-09-27" version="1">MDMNKGLLVAIAVLALFVLLLLFALYIAYRKYKVCSNQMNCLSEELIVGEKRYNTALGKLEKEVHCLTKQLAALRNENAKMLLALEQSEKSEVAFEHDSALERQLQLSNKQMVLKNCAKLKDLASRLRTIVSEAQARHEDDVMQIEHIISAAFNSMVSCYKSMLGNSCHQQVKIAMKDLESSIRCVKKHLYVLCDKLGEGFISAEEDISDEVFNPVIQIVNHAQKKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRA0</accession>
    <name>Q3YRA0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0723</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304832.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68755">
        <property type="protein sequence ID" value="AAZ68755"/>
        <property type="gene ID" value="Ecaj_0723"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0723"/>
    <dbReference type="HOGENOM" id="CLU_1118800_0_0_5"/>
    <dbReference type="OrthoDB" id="2149918at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="248" mass="28234" checksum="1DD8E8D68098B9B8" modified="2005-09-27" version="1">MCERSLNVIIALLALLALLIVVLLCAVIYKCHVDCQNERQRSSEIEEMKLLLRGQPQAILEPDVDREKIEKLCEEKFKNQLKEQQKANSDLQSKLKSLEKTNKHLAEVVSSLKKHGVIINRKNTELTEQCSMLRKNCAQLGVVCRKLGYDNKVLMRDAQVVFNAGFYSFKEAVDGMLQISAEIELLLLKRNERIDALTVGEIEYHLLTLRTILMDLEKQEKKSQKQPTQQVKAASVLPCSDQDNNKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRB2</accession>
    <name>Q3YRB2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304820.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68743">
        <property type="protein sequence ID" value="AAZ68743"/>
        <property type="gene ID" value="Ecaj_0711"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0711"/>
    <dbReference type="HOGENOM" id="CLU_282247_0_0_5"/>
    <dbReference type="OMA" id="ANASHKK"/>
    <dbReference type="OrthoDB" id="832300at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1040"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1107" mass="126027" checksum="610EC0D0DB0DAFBB" modified="2005-09-27" version="1">MLTGKFKRNAFIRHEVSCLALFLKSRGFTSKEILGFLSVVQSFHQSTPSFIIYTGYEFNIVSEKQINFVVKSFDHRNYSNYQYILSRLDESGELDLVCQAYLWTIQMYALLRFYCYACQLQFGSECYSKDEMVDALLEDLYDLCAGIMTRGEGGRDQIPPDVLLPYVERFVSLVFKVDYQSLRLKLCCIVENAYRGYSEARAGVALEAQDKEKRMLVLVMYKTAMSEFVSINSDEFSSLFKKQIFPMTCRFICNGINVLPELFISRLGIECYIDVINFYKDLFFVFDSRDLYDVYQNEDEFCSAIFNDICLSARGSLAAVGGNTKSYNPYSYQMSVFAFYDISLAIHEIFGTKRLKKLLQLEYMLQLYKTLLAEDLNEMHVTLVTLLGRFSFYYTDENGENKCALELLLELMSLCYYAAFINRVPMEQVSLCYSVCYNMMSEIADSGNFEISLQEYRKVFDDVVAELHCRSIDLSEVVFEKNLFFILVQHLLCGEVLGKKLTVSGLTYDVLLECKKTQELKAQQASIMLCRIFKKSNGIIGSCSSNNIGGLLGKLLFGNFLERNLILKMLNIFHIAYKRSKNSHNSRDIQKNLSQELASYFSVVLNLLKYVEASLPYDFSLDSIDGLSPDHCEIYSNINNMLNICAESLGISLCDLEMEHDVKFEKDFADILGAIPISLLNNESDVVNQVSFCKDFDKETCMLIMQLVVSYVCLYDSSKVKEDLCSANLSSPAFLLFNILTYPGLSSAFVNLCYKTSLEKSIVVKRTFIEMLQSFSSEGMLNDLPTTEVENKIIAKGKEIITERLVKLLIREDMSFNINEEVLKCACLSIIKLRELSHADVELDMRNARNIARTSGVFIIDDSLLSNALSYEDKSLGIDQAIKQNPLFSQFFSSSAADEISTVDKILSELGDLAESKKLDTTSMGIIELSDVKDESTSTSSLIITDDKVAHKYEKLSSDFYDIDFESEIDKVEISKMKDGLKSYFDSDFYRKAYLEKKKESTSLFKMDVCVYSAPVIALTMGCMSVLLLSIAFASQENTMLFVSCVVIAVICLVISILCCCITAMQIYNQKDDVAEEETCLLERENIFTKTQNPDIFRVKPDHNKMH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRB4</accession>
    <name>CTAA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heme A synthase</fullName>
            <shortName evidence="1">HAS</shortName>
            <ecNumber evidence="1">1.17.99.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytochrome aa3-controlling protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ctaA</name>
        <name type="ordered locus">Ecaj_0709</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of heme O to heme A by two successive hydroxylations of the methyl group at C8. The first hydroxylation forms heme I, the second hydroxylation results in an unstable dihydroxymethyl group, which spontaneously dehydrates, resulting in the formyl group of heme A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 A + Fe(II)-heme o + H2O = 2 AH2 + Fe(II)-heme a</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with CtaB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COX15/CtaA family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.17.99.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRB4"/>
    <dbReference type="SMR" id="Q3YRB4"/>
    <dbReference type="STRING" id="269484.Ecaj_0709"/>
    <dbReference type="EnsemblBacteria" id="AAZ68741">
        <property type="protein sequence ID" value="AAZ68741"/>
        <property type="gene ID" value="Ecaj_0709"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0709"/>
    <dbReference type="eggNOG" id="COG1612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017627_0_0_5"/>
    <dbReference type="OMA" id="YWWEWGH"/>
    <dbReference type="UniPathway" id="UPA00269">
        <property type="reaction ID" value="UER00713"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01665">
        <property type="entry name" value="HemeA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023754">
        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289">
        <property type="entry name" value="PTHR23289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000349030" description="Heme A synthase">
        <location>
            <begin position="1"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01665"/>
        </source>
    </evidence>
    <sequence length="347" mass="39834" checksum="5F2566F9744E5696" modified="2005-09-27" version="1">MKIADNTEHVCVSVKVWLCICSIGILLMVLVGGITRLTHSGLSITEWNPVIGIFPPVSEKEWIAEKMKYMSTPEFKCLNFNITFQEFKKLYLIEYFHRLLGRIVGLIFLLPFLYFTYKKKLNKNLIVNFIIICVLILFQGVMGWLMVKSGLIDRPHVSHYRLTAHLLLALLIFYLLWRQFLSAVILNVTCNLKINNTLIFYVISILIVIQITFGSLVAGLNAGLLHKTVPFLEGRFMLEDLLFMKPLWSNIFDNPITVQFIHEVIAVLILVIVSATLLILRLNFFPSYLLLVCLLIQLTFGILTFIYNVPIALASLHQVTAFILFAINTYLLHCVKLLKLQHVKFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRC4</accession>
    <name>Q3YRC4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0699</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family. DsbE subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304808.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0699"/>
    <dbReference type="EnsemblBacteria" id="AAZ68731">
        <property type="protein sequence ID" value="AAZ68731"/>
        <property type="gene ID" value="Ecaj_0699"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0699"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_19_4_5"/>
    <dbReference type="OMA" id="CKEEHPL"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03010">
        <property type="entry name" value="TlpA_like_DsbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004799">
        <property type="entry name" value="Periplasmic_diS_OxRdtase_DsbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="23"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="165" mass="18846" checksum="358C44957F492282" modified="2005-09-27" version="1">MKIIATALLVCFFTFVAIFTKVLLNKNTAPDNTNRIVLPILFTEDQVFDTNDLIGHPYILNVFASWCTTCQEEHKLWVEVAEKKTIKIYGINYLDTEHKVKEWLKKHGDPYTKIAKDYSGKMGSVLGVTAVPETFVFDKNGKMILQIHGNISKDILENQILKLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRC6</accession>
    <name>Q3YRC6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TRAP C4-dicarboxylate transport system permease DctM subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304806.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0697"/>
    <dbReference type="EnsemblBacteria" id="AAZ68729">
        <property type="protein sequence ID" value="AAZ68729"/>
        <property type="gene ID" value="Ecaj_0697"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0697"/>
    <dbReference type="eggNOG" id="COG4666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007041_2_0_5"/>
    <dbReference type="OMA" id="QWITTEG"/>
    <dbReference type="OrthoDB" id="271112at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010656">
        <property type="entry name" value="DctM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021814">
        <property type="entry name" value="DUF3394"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011853">
        <property type="entry name" value="TRAP_DctM-Dct_fused"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06808">
        <property type="entry name" value="DctM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11874">
        <property type="entry name" value="DUF3394"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02123">
        <property type="entry name" value="TRAP_fused"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="531"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="562"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="589"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="619"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="659"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="686"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="717"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="854"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="DctM" evidence="2">
        <location>
            <begin position="138"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="883" mass="97911" checksum="2BA14A0F34694C9D" modified="2005-09-27" version="1">MKKEAEHIQHLIDRELASSRPIDDGNKITSYTISFIAFLWACFQLFIASPLPFWLVDHGLEWAVFPDVKSRAIHLAFAFSLLFLFLPISKNSNKTLKALDCVSAVLSCISVLYIVVFYEDLSFRIAMPNDLDLIMAFLGLVFLLEGARRAIGAPIAIIALMFLMYAYFGNSMPDIIAHKGHNFSAIASHAWLSSEGVFGVALAVSSNFVFLYVLFGAFLDKAGAGNFFIKLSFALLGRFVGGPAKAAVIASGFMGMMSGSSVANTITIGSFTIPLMKKMGLSSEKAAAIEVSAGVNGQIMPPVMGAAAFLMSEFLSIPYATIVKYAFVPAVMVYVTLLYIVHLEACKLDMRPVIDLTKARSIYFSILRFFISVTCLVIFSCVVYVLIEGISIYGFYIPGIKAIFLDKSIYCISLILISVYIGVLFYQFKGERKRTVENDEEENKCPRLSETFKYGIHYFIPIFILVWCLIVEKLSSALSCFWTNLFLIFMLLAKDTIYALFDKRIMNLCSVFYGSMKQVYEAMVVASKNMIAIAVATGAAGIIVGAVSLTGFGLSISGLLDSIAHGSLFFTLLVTAVICIVLGMGMPATGCYIIVSTLMAPILTSVIHKSGFYVPQIAIHLFVFYFGIMADVTPPVGIASYAAAAIAKANAFKTGVQSFLYNIRTMIVPFLFIFNTELILYDVTNIWDNVFVIFVSLVGILSISAAMQGYFIRKNKLYESIMLLMLGIILIMPNYIAKLFVSPYDTIPFSKLHDKFVVLKDSAMKFTVESRKQSDGIRKEVIVPLKDKIQGVEFQGTLFDLLDKNIGITVDPNGTSNNRLEIKDIASWSYIEPSDMDESYCIVELALSLKKNYHVTYVYLIAIILLVLIIFLQTRKRDLVKRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRC8</accession>
    <name>RS6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsF</name>
        <name type="ordered locus">Ecaj_0695</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds together with S18 to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304804.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRC8"/>
    <dbReference type="SMR" id="Q3YRC8"/>
    <dbReference type="STRING" id="269484.Ecaj_0695"/>
    <dbReference type="EnsemblBacteria" id="AAZ68727">
        <property type="protein sequence ID" value="AAZ68727"/>
        <property type="gene ID" value="Ecaj_0695"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0695"/>
    <dbReference type="eggNOG" id="COG0360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113441_5_3_5"/>
    <dbReference type="OMA" id="PSHIYEC"/>
    <dbReference type="OrthoDB" id="1776954at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00473">
        <property type="entry name" value="bS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00360">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_S6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020814">
        <property type="entry name" value="Ribosomal_S6_plastid/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_S6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="PTHR21011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="SSF54995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000229540" description="30S ribosomal protein S6">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00360"/>
        </source>
    </evidence>
    <sequence length="109" mass="12653" checksum="3AA4518A1357B5F3" modified="2005-09-27" version="1">MPLYEFTFIAQQGLTQYELEGLVKGLSSLLTKNGAELLKYEYWGLLDFAYTIDKMNKGHYCMIYIKATPSSMDEFKRKVRLNEDVLRFLCLKKDKLPKGDSLMIQANQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRD5</accession>
    <name>Q3YRD5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 19">Succinate dehydrogenase flavoprotein subunit</fullName>
            <ecNumber evidence="8 19">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">Ecaj_0688</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>a quinone + succinate = a quinol + fumarate</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 19">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and a hydrophobic anchor protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 19">Cell inner membrane</location>
            <topology evidence="4 19">Peripheral membrane protein</topology>
            <orientation evidence="4 19">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 19">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="8 19"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304797.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0688"/>
    <dbReference type="EnsemblBacteria" id="AAZ68720">
        <property type="protein sequence ID" value="AAZ68720"/>
        <property type="gene ID" value="Ecaj_0688"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0688"/>
    <dbReference type="eggNOG" id="COG1053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_6_1_5"/>
    <dbReference type="OMA" id="DPIPIQP"/>
    <dbReference type="OrthoDB" id="153138at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 19" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 19" id="KW-0274">FAD</keyword>
    <keyword evidence="12 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="15 19 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-0813">Transport</keyword>
    <keyword evidence="11 19" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="20">
        <location>
            <begin position="14"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="21">
        <location>
            <begin position="464"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="18">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00019965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="AAZ68720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="598" mass="65715" checksum="25FE1FD71C6E73FC" modified="2005-09-27" version="1">MSSSAYDIIDHEYDVVVVGAGGAGLRATFGMTNVGLSVACISKVFPTRSHTVAAQGGISAALGNVSEDDWRWHMFDTVKGSDWLGDQDAIEYMCKNAPEAVIELERYGVPFSRTSEGKIYQRPFGGMTTEFGKGKPAVRTCAASDKTGHAILHTLYQQSLKYNAKFFVEYFAIDLIMDEEGQCKGVLAWSLCDGTLHRFRAHVVVLATGGYGRIYFSATSAHTCTGDGGGMVSRINLPLEDMEFVQFHPTGIYGAGCLITEGCRGEGGYLINSEGERFMERYAPKAKDLASRDVVSRAMTLEIREGRGVGPKKDHVYLSVSHLGAKVISERLPGISETARTFAGVDVTKDPIPVLPTVHYNMGGIPTNYYGEVVTLSNGQQKVVPGLFAIGEAACVSVHGANRLGSNSLLDLVVFGRAAAIRAKELIKPGMLHVPVKKSSEDWIIDRFDALRFSKGSLRVSEIRNNMQNVMQNHAAVFRTAEVLEEGKKNIKNVAESVSEIAVEDRSMIWNSDLVEALELTNMMPQAVVTMECAANRQESRGAHAREDFPERDDENWMKHSLAWYNPVDCSVNIKYKDVAKTTLTDDVQYFPPQKRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRF7</accession>
    <name>Q3YRF7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phage integrase, N-terminal SAM-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0666</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304775.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0666"/>
    <dbReference type="EnsemblBacteria" id="AAZ68698">
        <property type="protein sequence ID" value="AAZ68698"/>
        <property type="gene ID" value="Ecaj_0666"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0666"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_0_5"/>
    <dbReference type="OMA" id="HSFASHM"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="4"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="116"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="310" mass="36054" checksum="1B3D1A96AFCC2C11" modified="2005-09-27" version="1">MKDEKLCIIVNEWVSWLEKERRYSVNTVDAYVRDVNKFLGFLYTYVLRPVTLEDVVNIKVADLRKWFTFRYQANVEAITNARSLSALKNFFRYLSRTYDVDNQSIFCLSRPKLKSALPKTLGQFHIENIVNYYSLHDNWVFKRDFAIIMLLYGCGLRISEAVGLKFQDVRTGEILITGKGNKERILPILPVVRNSLDEYIKFCPYHTKLNSVGNEYIFVGVNGKKLGRTYFANRIQKICEKIGLPDTTTPHVFRHSFATHLLLSGADIRSIQELLGHANLSTTQIYTHLDHKSIIDHYKNFHPQVIKKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRF8</accession>
    <name>Q3YRF8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="3 12">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 12">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 12">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">purM</name>
        <name evidence="15" type="ordered locus">Ecaj_0665</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="3 12"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304774.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0665"/>
    <dbReference type="EnsemblBacteria" id="AAZ68697">
        <property type="protein sequence ID" value="AAZ68697"/>
        <property type="gene ID" value="Ecaj_0665"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0665"/>
    <dbReference type="eggNOG" id="COG0150">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_5"/>
    <dbReference type="OMA" id="EPLFMTD"/>
    <dbReference type="OrthoDB" id="923547at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="PTHR10520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="13">
        <location>
            <begin position="58"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="14">
        <location>
            <begin position="174"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAZ68697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="342" mass="37378" checksum="E49BCC17BB576EB5" modified="2005-09-27" version="1">MTTYSSSGVDIESGNSLVEKIKPIASSTFRPGVLDSIGSFGALFDISKIKEYNKPVLVSSTDGVGTKLCIAQEVNNHKTIGIDLVAMCVNDVLAQGAEPLFFLDYFATGQIDHNIALEVINSIAVGCKKANVALIGGETAEMPGMYSDNKYDIAGFAVGIIEAENILPKSNNIKAGDIILGLIASGLHSNGFSLIRKIISDNKINYHDICPWSNQTWANYLLTPTRIYVNSLLPTISLVNGLAHITGGGFIYNIPRIIPNHLSATIDLNSWKMPQIFHWLNTEMQIHQMELLKTFNCGIGMILVIPQENECQVLSLLKGIDESVYKIGEITERTTEDQVIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRG1</accession>
    <name>Q3YRG1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal silencing factor RsfS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rsfS</name>
        <name evidence="3" type="ordered locus">Ecaj_0662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with ribosomal protein L14 (rplN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Iojap/RsfS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304771.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0662"/>
    <dbReference type="EnsemblBacteria" id="AAZ68694">
        <property type="protein sequence ID" value="AAZ68694"/>
        <property type="gene ID" value="Ecaj_0662"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0662"/>
    <dbReference type="eggNOG" id="COG0799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092688_6_1_5"/>
    <dbReference type="OMA" id="VVHVFQK"/>
    <dbReference type="OrthoDB" id="1990650at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090071">
        <property type="term" value="P:negative regulation of ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01477">
        <property type="entry name" value="Iojap_RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004394">
        <property type="entry name" value="Iojap/RsfS/C7orf30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043">
        <property type="entry name" value="PTHR21043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00090">
        <property type="entry name" value="rsfS_iojap_ybeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="118" mass="13525" checksum="249091E0C78BE676" modified="2005-09-27" version="1">MSPSKSETNSTEELKNLITCILDKHKASNIVTMNISDKSNLAEYLIIASGESSYHVKSLAEKIKKEIKPYKKFSIEGLKDGNWVVLNCCNILVHIFKPEVREYYQLESLWNSQIKEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRM9</accession>
    <name>Q3YRM9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Adenylate kinase</fullName>
            <shortName evidence="6">AK</shortName>
            <ecNumber evidence="6 8">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">adk</name>
        <name evidence="9" type="ordered locus">Ecaj_0592</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="6 8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304704.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0592"/>
    <dbReference type="EnsemblBacteria" id="AAZ68626">
        <property type="protein sequence ID" value="AAZ68626"/>
        <property type="gene ID" value="Ecaj_0592"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0592"/>
    <dbReference type="eggNOG" id="COG0563">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032354_1_2_5"/>
    <dbReference type="OMA" id="MSPVIAW"/>
    <dbReference type="OrthoDB" id="1491686at2"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00649"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="PTHR23359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMP" evidence="6">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="62"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="221" mass="24746" checksum="E7679E2DC62B8BF5" modified="2005-09-27" version="1">MENKINILIFGPPGSGKGTQCHILSETYSSISIISTGDLLRNEAKLDTDDGKRIKKIIESGDLVDDDLVCKMFAKSISKIKNGFLLDGFPRNLSQAEFLTKILAEYDSQIDIVIQLQLDLEVIKSRLSSRILCKNCGQVSNLSFFGKKIDEITCSFCNSNKLDCRYDDKLEVIVKRVDKYNEEIKKLEDYYCDKIVCIDAGKSIEGVACDIKEKIAPLIIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRN8</accession>
    <name>RL34_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmH</name>
        <name type="ordered locus">Ecaj_0583</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRN8"/>
    <dbReference type="SMR" id="Q3YRN8"/>
    <dbReference type="STRING" id="269484.Ecaj_0583"/>
    <dbReference type="EnsemblBacteria" id="AAZ68617">
        <property type="protein sequence ID" value="AAZ68617"/>
        <property type="gene ID" value="Ecaj_0583"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0583"/>
    <dbReference type="eggNOG" id="COG0230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129938_2_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00391">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000271">
        <property type="entry name" value="Ribosomal_L34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020939">
        <property type="entry name" value="Ribosomal_L34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14503">
        <property type="entry name" value="PTHR14503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00468">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01030">
        <property type="entry name" value="rpmH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00784">
        <property type="entry name" value="RIBOSOMAL_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000013335" description="50S ribosomal protein L34">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00391"/>
        </source>
    </evidence>
    <sequence length="44" mass="5313" checksum="022C21F7EC34CB39" modified="2005-09-27" version="1">MKGTFQPSRIVRKRRHGFRSRMSTKMGRRILNRRRAQGRRVLCA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-24" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRP2</accession>
    <name>DAPB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <shortName evidence="1">HTPA reductase</shortName>
            <ecNumber evidence="1">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapB</name>
        <name type="ordered locus">Ecaj_0579</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304691.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRP2"/>
    <dbReference type="SMR" id="Q3YRP2"/>
    <dbReference type="STRING" id="269484.Ecaj_0579"/>
    <dbReference type="EnsemblBacteria" id="AAZ68613">
        <property type="protein sequence ID" value="AAZ68613"/>
        <property type="gene ID" value="Ecaj_0579"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0579"/>
    <dbReference type="eggNOG" id="COG0289">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047479_2_1_5"/>
    <dbReference type="OMA" id="HHPNKAD"/>
    <dbReference type="OrthoDB" id="803114at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016726">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00102">
        <property type="entry name" value="DapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="PTHR20836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000093967" description="4-hydroxy-tetrahydrodipicolinate reductase">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="99"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="164"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="267" mass="29171" checksum="D6052ED8E161BD59" modified="2005-09-27" version="1">MTKVNIGIVGCLGKQGKRLVAEISASPNAQVSAGLVRPGNPYVGQVLGEIVGCNCSAKAIDSLEHLFDVSDIVVEFTNPQTLLECIKMAESKKKPLLSGTTGPAATEMVFEDYIKNIPFLWTTNVSFGVNILAKLVEEAARKLFDYDIEVWEMHHRYKKDSPSGTSLILGRAAAKGRNVPFKKAQYVSGTPQEARQDVNTIGYAVSRGGSDLSDHRIMFVSDEEMIDFNHRTLNKNLYAKGALKAALWLVKQPPGVYTMSDMMAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRP4</accession>
    <name>Q3YRP4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Queuine tRNA-ribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Guanine insertion enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tgt</name>
        <name evidence="6" type="ordered locus">Ecaj_0577</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>7-aminomethyl-7-carbaguanine + guanosine(34) in tRNA = 7-aminomethyl-7-carbaguanosine(34) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:24104"/>
            <dbReference type="Rhea" id="RHEA-COMP:10341"/>
            <dbReference type="Rhea" id="RHEA-COMP:10342"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:58703"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82833"/>
            <dbReference type="EC" id="2.4.2.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the queuine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.29" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304689.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0577"/>
    <dbReference type="EnsemblBacteria" id="AAZ68611">
        <property type="protein sequence ID" value="AAZ68611"/>
        <property type="gene ID" value="Ecaj_0577"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0577"/>
    <dbReference type="eggNOG" id="COG0343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022060_0_2_5"/>
    <dbReference type="OMA" id="GIDLFDC"/>
    <dbReference type="OrthoDB" id="1165356at2"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:queuine tRNA-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101030">
        <property type="term" value="P:tRNA-guanine transglycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00168">
        <property type="entry name" value="Q_tRNA_Tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004803">
        <property type="entry name" value="TGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00430">
        <property type="entry name" value="Q_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TGT" evidence="5">
        <location>
            <begin position="15"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="93"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding" evidence="4">
        <location>
            <begin position="267"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding; important for wobble base 34 recognition" evidence="4">
        <location>
            <begin position="291"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="396" mass="44903" checksum="E12B303555E0B45B" modified="2005-09-27" version="1">MYKNFDFKIIHQYNNARLGTILTPHGTINTPAFIFCATKGAIKSVDISKIKESDTQIILSNTYHLMLQPGADIIEKLGGLHKVTGWNKPMLTDSGGYQIFSLGYGSVSHEIKNAGRSYGSRTLVSISEEGAVFKSYINGNLYHLTPEKSIQIQQKLGADLIVVLDECTPFHISYKYTKDSMNMSHRWAVRCIEQFKKNNDNSQALYGIIQGGIYEDLRIDSCNFTNDMPFFGHAIGGSLGACKQQMYDIVSLTTSKLDKNRTIHLLGIGNIDDIFQGVKSGIDTFDCVYPTRIARHGGALVKPKYRKEETRTKEYINLRNQRFKIDTNPIESDCLCFTCCNHSRGYIHHLLKAQELLAYSLITIHNIYFMNNLMQSIRKAILNNTLEEEQNQWVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRP9</accession>
    <name>Q3YRP9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304684.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68606">
        <property type="protein sequence ID" value="AAZ68606"/>
        <property type="gene ID" value="Ecaj_0572"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0572"/>
    <dbReference type="HOGENOM" id="CLU_1822337_0_0_5"/>
    <dbReference type="OMA" id="FWQMFVS"/>
    <dbReference type="OrthoDB" id="1820253at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="141" mass="16638" checksum="889652AC9DDA1D94" modified="2005-09-27" version="1">MYFDVLISLVIALGALGICIALFSLYKNYVHEHILKIFVGDDCEEQLTDEEKYKKYIEKKQKELIKHQMLVRDITQGNTEYKSNIKIVDIMEPIGHWTRFVMSEKLQRFAGLKFDKEETGFWQMFVSMRSLSQGKYKGRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-04-07" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRQ4</accession>
    <name>Q3YRQ4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="4 9">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0566</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="4 9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0566"/>
    <dbReference type="EnsemblBacteria" id="AAZ68601">
        <property type="protein sequence ID" value="AAZ68601"/>
        <property type="gene ID" value="Ecaj_0566"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0566"/>
    <dbReference type="eggNOG" id="COG0209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_7_0_5"/>
    <dbReference type="OMA" id="LEIWHID"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="PTHR11573"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="SSF48168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonuc_red_lgN" evidence="10">
        <location>
            <begin position="41"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="RIBORED_LARGE" evidence="11">
        <location>
            <begin position="109"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="RIBORED_LARGE" evidence="11">
        <location>
            <begin position="440"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="622" mass="71191" checksum="76B2879A6EDC3E05" modified="2005-09-27" version="1">MFYIVNTYNIYAFLQYVVLNDEKMVEIMRKVIVDYGRDNNLTSFGKAVLSDRYLLENEDYQQLFVRISEYYSDNPEHAQRLYDYMSQLWFMPATPILSNGGTNRGLPISCFLNETEDSLRGIVNLWNENVWLASKGGGIGSYWGNLRSIGEKVRGSGKTSGIIPFIVVQNALTLAISQGSLRRGSSAVYLPVWHPEIEEFLDLRKPTGGDPNRKALNIHHGVVLSDKFMKCVENDEPWDLISPKDQSVIFTVKARDIWIKILTTRVETGEPYILFIDQVEQNKPEIYKKLNLNIKMSNLCTEITLTTGYDHLNKSRTAICCLASLNLEYYEKWKDNSLFIEDVMRFLDNVLTDFIEKAPNDMERAKYSAMRERSIGVGVMGFHSFLQSKMIPFESVAATIWNKKIFSHIKRHADAASYNIAMEKGSCPDAQDAGVIERFVNKLSIAPTASISIIAGNTSPGIEPYAANVFTHKTLTGSFIVRNKFLQKLLIVKGKDNEQVWSSISTNEGSVQHLDFLTEEEKLIFKTAYELDQRWIIEHTSDRSKYICQAQSVNIFLPANVHKRYLHKIHFLAWKKGLKSLYYCRSKSIQRADKVSHGALEKSVEQYKQITNFDYNECLSCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRR8</accession>
    <name>Q3YRR8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH/Ubiquinone/plastoquinone (Complex I)</fullName>
            <ecNumber evidence="4">1.6.5.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0552</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.6.5.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304665.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0552"/>
    <dbReference type="EnsemblBacteria" id="AAZ68587">
        <property type="protein sequence ID" value="AAZ68587"/>
        <property type="gene ID" value="Ecaj_0552"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0552"/>
    <dbReference type="eggNOG" id="COG0651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_519667_0_0_5"/>
    <dbReference type="OMA" id="LINCACF"/>
    <dbReference type="OrthoDB" id="1887859at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="343"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="462"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="505"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="3">
        <location>
            <begin position="83"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="531" mass="59835" checksum="91EC16EF7EDEADB5" modified="2005-09-27" version="1">MSKYFTTRCSSIAMIHWLILFVFVVLELYLYDAKIDEYNYSLILFYVFSFIIAQIFLYIWNLKKHETILYLLYYLSSITTISSKSLLSLVISFEFMSLSALMIIAADSVGNNKKAVIHYSCVHFLAGVLLLIGSFGGVLSVVKHSYDDYCRLCFLVGLLINCACFPLSSWVTDSYSSALNNSIIVLSVFTTKVSAFVLLFFFQGESILLFLGIATSVYGIVFSVLENNIKRLLCYNLVGQMGLVITAIGFSYSGDVDVHGVIVLQIVLSIVYQTLLFMVAMSVINSTQKFNLNEVGGLFKKMPMEAICSAVAIFTMGALPGTGGFVSKLLITHNVDNFDPTNVLISKLFLGCSILLFISVGVKFFWYVFISKSKENTVIKKVCIASRLSMLILTLVCIFFGVFSSYYVFHNLDPETLDSIILQCELIFGSILFFIVFRSVFSGRVNFNMDIDWVYRVLFMRIIYIICDFLLYIFSILVTMFSYVFSNKMLLCSKSGKEVLNIASVNPLGFMILLSMLFVIVMVIVYLCLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRS1</accession>
    <name>Q3YRS1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA_pol_B_exo2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304662.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0549"/>
    <dbReference type="EnsemblBacteria" id="AAZ68584">
        <property type="protein sequence ID" value="AAZ68584"/>
        <property type="gene ID" value="Ecaj_0549"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0549"/>
    <dbReference type="eggNOG" id="COG3298">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069554_0_0_5"/>
    <dbReference type="OMA" id="VLHYRGL"/>
    <dbReference type="OrthoDB" id="1985371at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019288">
        <property type="entry name" value="3'-5'_exonuclease_PolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10108">
        <property type="entry name" value="DNA_pol_B_exo2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_pol_B_exo2" evidence="1">
        <location>
            <begin position="51"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="275" mass="31994" checksum="5D9982DD5C0E0943" modified="2005-09-27" version="1">MLNSLLVFDIETVPDVDVCRNLFSDFDDTIELSEKREALTSYHLEITDGKNPFLRQPFHKIVAISFLKADIKRIGEYESFHLQEIRSGGKVDSSEKDLLKGFFQYVSSLRPRFVSFNGRTFDFPVLKYRAMIHGVQAGSLYKIGDKWNNYFQRYSVDWHCDLLDYLSDFGTSARVKMNEVCSVLNFPGKFGIDGSKVTELYDNNKISAIRDYCETDVINTYLVYLRVMHYQDRVTTLSYNDSISDLLSYLDSGEKEHFKQFKVEWNKACCGNFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRU9</accession>
    <name>Q3YRU9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Secretion protein HlyD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304634.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YRU9"/>
    <dbReference type="STRING" id="269484.Ecaj_0520"/>
    <dbReference type="EnsemblBacteria" id="AAZ68556">
        <property type="protein sequence ID" value="AAZ68556"/>
        <property type="gene ID" value="Ecaj_0520"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0520"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_0_1_5"/>
    <dbReference type="OMA" id="IKVTPAM"/>
    <dbReference type="OrthoDB" id="1432684at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HlyD_D23" evidence="2">
        <location>
            <begin position="76"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="356" mass="40134" checksum="CCD40ED5C9EFCB1A" modified="2005-09-27" version="1">MLRKLLKLKLQYKIAFCILLLAIFWISSGVFKKSVQTERDVLHNNNNAPNIQVIESHAQEKLVYLSLIGEVEAFHHVNVISEVAGKISDILVKDGDYVTKGNLLVKVEKYEKEAQLKQAQALLRQRELEYKASQSLNEHGYRSEINDALSFAALESAKADVKRAQINLGSTEINAPFSGFIDKINVQVGELLSPGISIVHLVNFDKIRVVTYVPEKYLNKLKLGSICKILMAQNKEIDAPITFISKVINSNTRTYRVEMVLDNDDSKVFAHGAVYSVKIPLGNYKAHRISSSALDLQDNGDLGIKVIIDERVKFIPIEIIDSEYNGDVWIVNSPDNIKLITLGHEYVKENTHIEYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRV2</accession>
    <name>RS2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsB</name>
        <name type="ordered locus">Ecaj_0517</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304631.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRV2"/>
    <dbReference type="SMR" id="Q3YRV2"/>
    <dbReference type="STRING" id="269484.Ecaj_0517"/>
    <dbReference type="EnsemblBacteria" id="AAZ68553">
        <property type="protein sequence ID" value="AAZ68553"/>
        <property type="gene ID" value="Ecaj_0517"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0517"/>
    <dbReference type="eggNOG" id="COG0052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040318_2_1_5"/>
    <dbReference type="OMA" id="SYIDRAY"/>
    <dbReference type="OrthoDB" id="1623045at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_S2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_S2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_S2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_S2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="PTHR12534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="SSF52313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000003955" description="30S ribosomal protein S2">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="259"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="291" mass="32693" checksum="FFD0BB0F763560F2" modified="2005-09-27" version="1">MVNLPKFTMRDLVESGVHFGHKASRWNPKMAPYIYGVYNDIHIINLQNTVVLLKNALKALYDVVLKRGRVLFIGTKVQASAIIADEAIRCGQYYVNNRWLGGMLTNWETISLSIKKLKEYEKLIENVDNQFTKKELLLFEKKRAKLDRSIGGICNMGGLPHVLFVIDTNKEHIAIKEANKLNIPVIAVLDTNSDPTGIDYPIPGNDDAVRSIDFFCKIVSDTILEAIRSDLAKSGINVDGIKDFSVEKRDDLLRTSNRDNKNNKNNNNTDNTDNAASIKEEDLIGGSNNEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRV3</accession>
    <name>EFTS_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor Ts</fullName>
            <shortName evidence="1">EF-Ts</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tsf</name>
        <name type="ordered locus">Ecaj_0516</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EF-Ts family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304630.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRV3"/>
    <dbReference type="SMR" id="Q3YRV3"/>
    <dbReference type="STRING" id="269484.Ecaj_0516"/>
    <dbReference type="EnsemblBacteria" id="AAZ68552">
        <property type="protein sequence ID" value="AAZ68552"/>
        <property type="gene ID" value="Ecaj_0516"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0516"/>
    <dbReference type="eggNOG" id="COG0264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047155_2_0_5"/>
    <dbReference type="OMA" id="DAGMMDC"/>
    <dbReference type="OrthoDB" id="1405357at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00050">
        <property type="entry name" value="EF_Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036402">
        <property type="entry name" value="EF-Ts_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001816">
        <property type="entry name" value="Transl_elong_EFTs/EF1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014039">
        <property type="entry name" value="Transl_elong_EFTs/EF1B_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018101">
        <property type="entry name" value="Transl_elong_Ts_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741">
        <property type="entry name" value="PTHR11741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00889">
        <property type="entry name" value="EF_TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54713">
        <property type="entry name" value="SSF54713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00116">
        <property type="entry name" value="tsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01126">
        <property type="entry name" value="EF_TS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01127">
        <property type="entry name" value="EF_TS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241479" description="Elongation factor Ts">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in Mg(2+) ion dislocation from EF-Tu" evidence="1">
        <location>
            <begin position="79"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00050"/>
        </source>
    </evidence>
    <sequence length="288" mass="31606" checksum="064D5F771FD9032F" modified="2005-09-27" version="1">MKIDISAIKKLRDLTGAGVGDCKEALSSCDGDIEKAKNYLREQGIAKAYKKSTKDVSDGLIAIHVDGNKGAILEVNSETDFVARNEKFQKLVLNLVSLANQYATESIEDFLKHEYISGTSVHDEIMSNIAIIGENIHLNKIGCLSVNSGVVSGYIHNPVIDNLGKIGAIVALESNGDSEKLKVLAKQIAMHIVAAKPEALSLDVLDKDLLNKEREIIKKQVDQLNKPAAVAEKIIDGRMAKFYQDVVLLDQVFVMDSQLTISELVKRKESELAETINLIGYKLFVINK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-02-21" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRX7</accession>
    <name>RSMH_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase H</fullName>
            <ecNumber evidence="1">2.1.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA m(4)C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (cytosine-N(4)-)-methyltransferase RsmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmH</name>
        <name type="synonym">mraW</name>
        <name type="ordered locus">Ecaj_0491</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = H(+) + N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10287"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74506"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.199" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304606.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRX7"/>
    <dbReference type="SMR" id="Q3YRX7"/>
    <dbReference type="STRING" id="269484.Ecaj_0491"/>
    <dbReference type="EnsemblBacteria" id="AAZ68528">
        <property type="protein sequence ID" value="AAZ68528"/>
        <property type="gene ID" value="Ecaj_0491"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0491"/>
    <dbReference type="eggNOG" id="COG0275">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038422_1_1_5"/>
    <dbReference type="OMA" id="NPAKRTF"/>
    <dbReference type="OrthoDB" id="1272633at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="PTHR11265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004486">
        <property type="entry name" value="MraW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81799">
        <property type="entry name" value="SSF81799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00006">
        <property type="entry name" value="TIGR00006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000223543" description="Ribosomal RNA small subunit methyltransferase H">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="30"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01007"/>
        </source>
    </evidence>
    <sequence length="297" mass="33349" checksum="862EEA1C7C2CC8B3" modified="2005-09-27" version="1">MHTPVLLKEMLEILDPQDGKIYVDATFGAGGYTKAILNAANCKVCAIDQDKHTSIFYEELANNFPNRIHFFINKFSQIKQVLLGAQLERVDGVVFDIGVSSMQLDDANRGFSFSKNGPLDMRMSTSNSIDASVFVNTVSEEEIANIIYQYGGEKYSRKIAKAIINFRKKKVIETTGELASIVRSVVSRSKNHDINPATRTFQAIRIWVNKELQELEQGIMCAADLLNPGGKIIVVSFHSLEDRIVKMIFKSLCSDEISLKLNTGFQLINKKIVRPSFEEILNNPRSRSAKLRAILKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS01</accession>
    <name>IF2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infB</name>
        <name type="ordered locus">Ecaj_0467</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304582.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS01"/>
    <dbReference type="SMR" id="Q3YS01"/>
    <dbReference type="STRING" id="269484.Ecaj_0467"/>
    <dbReference type="EnsemblBacteria" id="AAZ68504">
        <property type="protein sequence ID" value="AAZ68504"/>
        <property type="gene ID" value="Ecaj_0467"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0467"/>
    <dbReference type="eggNOG" id="COG0532">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006301_10_2_5"/>
    <dbReference type="OMA" id="VIFAMNK"/>
    <dbReference type="OrthoDB" id="347113at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="PTHR43381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="SSF52156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000228194" description="Translation initiation factor IF-2">
        <location>
            <begin position="1"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="346"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="355"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="380"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="402"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="456"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="492"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="355"/>
            <end position="362"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="402"/>
            <end position="406"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="456"/>
            <end position="459"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="848" mass="93816" checksum="59FD73ECC9C4A8E9" modified="2005-09-27" version="1">MNESKGAVDSGLMSGKTERTTLKLSDKLKLSSSIQQNTKFALNKSITTVEVRKSKKRRDIDNIEQASVVLQNDNVYQSDGDNNTLTIQEQISRMNALQNANIHEKKEEVSDDQTDKKEEVTNAETVGLLPVEENVNTDLVIEEGHSEKVEEVIEESVIEDQPNLEHLDVIEEKNGPNQSGDQNVDNSIVDLLQSKAVEEKKLKKYEKEHEEKKGNPKKGVSNHMYSKHVKLVIEEELEDNNKQIIQTHKTRKNRSVSSIKNKITRKVLIPKKITVQELASNMSERVKDVQNMLFQMGRRDIKPNDFLDSDQAAIIVEAFNHTFKLVNDGKLEDDLYSDGNDKELLPRAPVVTVMGHVDHGKTSLLDAIRESNVADGEFKGITQHIGAYQIILDGDKRITFIDTPGHEAFTAMRACGTNVTDIVVLVVAADDGIMPQTIESINHVKAANVAMIVAVNKIDKHDANLDKITNSLLNHGVVAESLGGDVIVVPVSAKEKINLDQLKSSILLMAELLELKAIYNTRASGVVIESKIDKNCGVVATLIVQKGTLKSGDIIVVGHNSYGKVRNMFNSDGRSQKVAIPSMPVKVLGLNNVPNSGSSFIVVDSEKQARELINYRQELFNASLEENAKSKMDASNILACDVVDELNVILKCDVMGSVEAICYSISKITHEDIKLNVLYKGVGNVTKSDVLLAETSNSIILAFNVKADAQVKELAKQRCVEINHYSVIYDIIDDVKRILSSMLKPLQQEVQVGALAIRKVFSSGNAGSVLGCYVTSGVVKKGSLVKLIRNNTIIHEGKIKVLRRFKDDVKEVSSGFECGILLDYSKEIYPESDIINVLEVIEEVRVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS03</accession>
    <name>Q3YS03_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integral membrane protein TerC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304580.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0465"/>
    <dbReference type="EnsemblBacteria" id="AAZ68502">
        <property type="protein sequence ID" value="AAZ68502"/>
        <property type="gene ID" value="Ecaj_0465"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0465"/>
    <dbReference type="eggNOG" id="COG0861">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064910_0_1_5"/>
    <dbReference type="OMA" id="IMFLDIV"/>
    <dbReference type="OrthoDB" id="1917125at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005496">
        <property type="entry name" value="Integral_membrane_TerC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03741">
        <property type="entry name" value="TerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="227" mass="25846" checksum="7CF67F6CD1709588" modified="2005-09-27" version="1">MENDIIKFFTLLMLEVVLGVDNIIFISLAVTKIQPELRNKAKYIGLSLALIMRIITLYGASYIISLNNPITSLLNFKLSTNNLFMIFGGTFLIYQSLSEIWNDISNKNHKISNLKSNFYIVILQIIIVDFIFSIDSILTAIGITHNIFIIQIVFAASIIFTILFLNYIIKVVTTHSNIKTIAVMFVLMLGIILVLDGIHIKVSHNYLYFTFIFSTITEVINIAKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS05</accession>
    <name>Q3YS05_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0463</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304578.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0463"/>
    <dbReference type="EnsemblBacteria" id="AAZ68500">
        <property type="protein sequence ID" value="AAZ68500"/>
        <property type="gene ID" value="Ecaj_0463"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0463"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_250450_0_0_5"/>
    <dbReference type="OMA" id="NFDCKMA"/>
    <dbReference type="OrthoDB" id="147328at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1328" mass="155000" checksum="F6745C66D69A4C4B" modified="2005-09-27" version="1">MLNFNKVPTSNTPSKLYLIIACVSLIMLLMFLLMRETGVLIEKNNIVSDIITGFFTSLLLFISTIFSVMYLYDCLIYKNNLINLFRKEHKTIIEPQQIELNKKFEHYVKWLLEFKKSQLDNLSDDVTLMMQQSSLNLEEYNRAIDELKALQNMQKEIITQSNNLAYKFQLYDYEYSNSLKFAQDDFVRQVMYERDSYSSFYDYLKELSVGLTEGDGDEPLFSISPRMIYINNLPPIVSVFKSASALLTVVGRLEADNPSLFSEYCQRFYNFDCKMALTNEISLSNTLRGDSRVYNDICDWIYNAELKYNMAYNFVTAKRRYCDSFVITRDLVSSSIDEFSSIDLDLQDTEYKMDRIFGMSSPVIEQLAEDLNVLKEDRQETLDNFRIEVTEELEDYTEGEVIASDKIQHELSSRSLGMNIVRFLQQEEALKALIEKRRAVMSSDGLLNKAEETLSYIKMRFDAINSHIAIQQCNLHKVTSEILSLLNEVLNKKRFIKTIDIRDKASHLFENLSQEENDQIELIQEIKSIVEQIVSITSSTENTLLKSLHDDLLSYLSLLNGMEKVFSVQELVQCKVKTSEEFLDTGQSNVQEILDVLYKRYDFLTKNFTIIQNILFSLDDIETINSMRLPSYRYIEQVYDLSKNLIELQDRYDTRCKLIDKLEDLLNAQLQVKDVSSYLHLLSLSDDEKRILVMQLSKQEKPVLLFKLLEEEGFTAIREKMTEQLNEKKEAISQVLSTFIEKKNQILEDQAITHLLNGKPINVRFIDHLERLADSLKLLSNEDGELIYDNGDIEEINTIVIWLKKISELLYNKTSAERARSTESYFQCLNDGFLTESRLFNVSNLKEKIQALMESPSILEQLSAVGYQFEEDQIFMINDLHFFLSLATFLKVQLNDLHKGTLGMILEIMDIEKQDKELLINTINQELRFLISNDEIEEMVDIFLREEHEELVKLNTLQSSVSKVKEEYDDIEVLLNKVFELLPDVQDETKNFIYRRLKNITPYSVDLVISDLRLYRLPLDEKLTKAETVDRLKVLIGCMIDDIDKISLPNVPEQHKRYRMYEEEVALHTQIKSDLEKLQEILQKKEIQSCDIIEAIQGLDKNVKRTFSINQQLSRLMSFITIRNEEYKKILQFKSYVAHFLSFIKLLVYCVVETLFFIRTTLVIPLDHKIHQQTKLVSCLKVKVKEKLNKVVKGLLSKKLYYPKDLKIVRMIKKKLIDHGIVCDAIQVLEDGVENRVKCSMDALEGLMLTREINYVEQLMENDESEKRSCFRKLQLYKVIKKFMDNSVDKESTQYLEKINVNSQQGSNTSLVSFCEYSDRAQSYACCM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS09</accession>
    <name>ATPB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit beta</fullName>
            <ecNumber evidence="1">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpD</name>
        <name type="ordered locus">Ecaj_0459</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS09"/>
    <dbReference type="SMR" id="Q3YS09"/>
    <dbReference type="STRING" id="269484.Ecaj_0459"/>
    <dbReference type="PRIDE" id="Q3YS09"/>
    <dbReference type="EnsemblBacteria" id="AAZ68496">
        <property type="protein sequence ID" value="AAZ68496"/>
        <property type="gene ID" value="Ecaj_0459"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0459"/>
    <dbReference type="eggNOG" id="COG0055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022398_0_2_5"/>
    <dbReference type="OMA" id="GFNMIMD"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000254255" description="ATP synthase subunit beta">
        <location>
            <begin position="1"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="183"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="507" mass="55076" checksum="F412A07749FD741B" modified="2005-09-27" version="1">MSSLANKAKSKGKSSKSKSNVNTQVDELSDSIGLVVRVMTAVVDIKFDGGKVPKILNALESKKFYNGKKLVLEVSQHISDNIVRCIALDSTDGLSRNDEFIDTRAPISVPVGRGTLGRVFDVLGNTIDDCGPLEESKYIIKPIYSEIPKLTDQKIATEILVTGIKVIDLLAPYLKGGKVGLFGGAGVGKTVLIMELIHNIAKAHRGVSVFAGVGERTREGNDLYHEMIESGVINLNEKDQSQAVLVYGQMNEPPGARLRVALSALTMAEYFRDSENQDVLFFVDNIFRFTQSGSEISALLGRIPSAVGYQPTLAAEMGAMQERITSTNNGSITSVQAIYVPADDLTDPAPATSFAHLDSTTVLSRQISELGIYPAVDPLDSTSQALSADIVGEEHYEVAKEVQRILQTYKSLQDIIAILGMDELSEDDKLIVARARKIQRFLSQPFHVAEVFTGSPGKFVSLEDTVSSFKGLVEGKYDHLPEAAFYMVGSIDDVIKKAELLQKEGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS16</accession>
    <name>Q3YS16_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sodium:dicarboxylate symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0449</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0449"/>
    <dbReference type="EnsemblBacteria" id="AAZ68489">
        <property type="protein sequence ID" value="AAZ68489"/>
        <property type="gene ID" value="Ecaj_0449"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0449"/>
    <dbReference type="eggNOG" id="COG1301">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019375_7_1_5"/>
    <dbReference type="OMA" id="YFCTTII"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42865">
        <property type="entry name" value="PTHR42865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="SSF118215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="426" mass="47904" checksum="AB07E6A0146E2D73" modified="2005-09-27" version="1">MINRNLLNITCNFVLLMIHKFSLKIFIPIILAIISYYVNQKWIFEFAKAFGDIFINLLKLISLPVVFFSITSTISGLANLIEIKNLIKKTIFYTISTTIVAAIIALTIYLLIDPSKKALVFNILNTNEHINTPDYLSYLISILPHNFIKVFLDNNVIGCVILAFLIGGSLLVLPDKNKRELLHKILDALFDTFLEIAKLILKLMPIALWSFITVLLYNTKEGYNLSSILKYLLCIMVANFIQAFVVLPLLLKLKKISVIKTFRGALPALTIAFFSKSSTATLPTTLRCAQDNLKISKKISSFILPVCTTINMNACAAFILITVFFVSEMNEYTFSIGEMFLWIFLATGAAIGNAGVPMGCYFMAMSYLISMKVPLSIMGIILPIYTIIDMFETAINVWSDVCIAQVINKEYSILTQENKKISHNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS18</accession>
    <name>Q3YS18_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA recombination protein RmuC homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0447</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA recombination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RmuC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68487">
        <property type="protein sequence ID" value="AAZ68487"/>
        <property type="gene ID" value="Ecaj_0447"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0447"/>
    <dbReference type="eggNOG" id="COG1322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024057_0_1_5"/>
    <dbReference type="OMA" id="GDSKMQG"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003798">
        <property type="entry name" value="DNA_recombination_RmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30563">
        <property type="entry name" value="PTHR30563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02646">
        <property type="entry name" value="RmuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="41"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="437" mass="50627" checksum="B6C64BF4C9D24447" modified="2005-09-27" version="1">MNMTITTICILEGLLLILVSYFIALKSRISKFDHRILCMIKDDLERKIQRLELENGDLKSKNNQFSIELQNMQSKITELEFEKKTLKYNIDLYKNEFASIDEKYKSHFENLANRIFDEKTDKFNKISQESLSKILNPLQEHINLFKKKVDDSFDVHNKEQFLLKQEIQRIVLANEKITLQAENLANVLKGNVKVQGNWGEMILEKILESSGLRKNQDYILHGTGMDLQNSEGNKQQPDAIVNLPENKHLIIDSKVSLTHYEKYFSTKSEESKDVLLKQFITSVRLHVNVLSNKKYQNIEKLKTPDFVLMFIPIEGAYSLAMQEDVMLHDYAWNKKIVIVCPSTLFATLRTIESVWRLERQNRNTMEIARQGGALYDKIVGFVSDMQKLGKQINILNGVYSDAMKKLSEGNGNILSRTENLKHLGVKTSKKILENFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS26</accession>
    <name>Q3YS26_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carboxyl transferase</fullName>
            <ecNumber evidence="3">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304557.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0437"/>
    <dbReference type="EnsemblBacteria" id="AAZ68479">
        <property type="protein sequence ID" value="AAZ68479"/>
        <property type="gene ID" value="Ecaj_0437"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0437"/>
    <dbReference type="eggNOG" id="COG4799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_6_2_5"/>
    <dbReference type="OMA" id="YAYAECT"/>
    <dbReference type="OrthoDB" id="886663at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="2">
        <location>
            <begin position="264"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="509" mass="56335" checksum="B04CA0B9CC02B8E3" modified="2005-09-27" version="1">MNFSGLQDLHDRKLKSFYGGGVSRIEKQHIKGKLTARERLTVLLDDNSFEEYGTFVEHRSVNFSMDKSKIPGDGVVVGYGTINGRKVCVYSQDFTVFGGSLSESNAKKICNIMDKAASLGLPIIGINDSGGARIQEGVDSLAGYGEIFQRNVNLSGVVPQISIIMGPCAGGAVYSPALTDFIFMVRNTSYMFVTGPDVIKKVMYEDVSQEDLGGAKVHSNKTGIADLVFNDEIEALLYVRKFMDFITSNNTELSKSQLVSCLDTEDLSLNTLIPKNSSTPYDMNELLEKVCDERLFYEIKPDFARNIIVGFGKIGGYKVGLIANQPLHLAGCLDIDASRKGARFIRFCDAFNIPIITFIDVPGFMPGVNQEHSGIIAHGAKLLYAYAEATVPKISIIVRKAYGGAYIVMNSKHLCGDINYAWPDAEIAVMGAEGAVEIIFRNETDKEKIQNIIDEYRTKIVNPYVAASRGYIDDIIIPSRTREYLFKSLQLLEKKKVNKIMRKHDNLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS27</accession>
    <name>Q3YS27_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0436</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304556.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0436"/>
    <dbReference type="EnsemblBacteria" id="AAZ68478">
        <property type="protein sequence ID" value="AAZ68478"/>
        <property type="gene ID" value="Ecaj_0436"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0436"/>
    <dbReference type="HOGENOM" id="CLU_118038_0_0_5"/>
    <dbReference type="OrthoDB" id="1822921at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="195" mass="21954" checksum="D5F4AF032E650F98" modified="2005-09-27" version="1">MRQLLLLMDKNGRLLLSDVSLYDNIGGKFIPRDPSLKEIVKEINESEEIDSCVAFDPYIYNAIFSNFTVISSQKFKNEQLILHGVDKTGTAVFLSLLLNRGEFKSFLSNINDTPCFFIDNNSYIDGGPTYERSDKQLFCDMKNNLVGLVPKTSDNKLCVTPFRFFNEDGGIVNSVLISCEISMQGAIGLRRVTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS28</accession>
    <name>Q3YS28_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304555.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68477">
        <property type="protein sequence ID" value="AAZ68477"/>
        <property type="gene ID" value="Ecaj_0435"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0435"/>
    <dbReference type="HOGENOM" id="CLU_118038_0_0_5"/>
    <dbReference type="OrthoDB" id="1822921at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="195" mass="22490" checksum="96158BD2E1306EB4" modified="2005-09-27" version="1">MQLLLFCDRNNKLFLYDMSLCKEVNGKFVPRDESIRTMVKDTGDDQQQSKDPYMNRFDLVGSCFSNFTFINSKKFYNEFVIMRGVNMHGENVCLSLSLFKNEFQSFLERSAFTPCFFVNDSNYVGGGPTHDRTNKQLFCDLKANLKDLVPKKPDINSNVISFKFFNENGKIVDTVLSLCEVLAPTHLTPKMIKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS32</accession>
    <name>Q3YS32_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304551.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68473">
        <property type="protein sequence ID" value="AAZ68473"/>
        <property type="gene ID" value="Ecaj_0431"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0431"/>
    <dbReference type="HOGENOM" id="CLU_628121_0_0_5"/>
    <dbReference type="OrthoDB" id="2112692at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021387">
        <property type="entry name" value="DUF3023"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11224">
        <property type="entry name" value="DUF3023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="436" mass="49360" checksum="FF48A50382C6468F" modified="2005-09-27" version="1">MWSVLKQKVLGGAGKEFDENQEKNKERVNKELLEKSKKRSLKKEEGLTQIETTQLQTIQELSTEEIPQTLADVKIECYDGNQEQEKALTTQESLEKKEEKYAEIDISQLQQVQELSTEKIYQEQREQHYTQTKLYEKAGARPKVTSGTVGKKIEKRGKKQPTASVPTESEIEEQKKAEVREAKQEEKALQEALSKLKLRPVSCVERMLSYYDEEKKKDYQELANMVQTITPNDINVKSTKCIGNTDPVSGKMYVSCNRDKSLKVEVKGDSLFIVNCRLSNKYLSENSRVNCAFPPKTPKQRKRKAIVYCFVDGNNTSKFLETCTTTSLSSYFLNSQCPFNFCNVKYVHFYTKHQNNKNLDLSDEKEALECFGGLTNVEFIDSRSSPSSSLSSSPSTSGSSSSHSSSPTESSSSGSPSSDFKSSFTSKFTRRQHHAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS40</accession>
    <name>NUOH_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit H</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoH</name>
        <name type="ordered locus">Ecaj_0422</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 1 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304543.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS40"/>
    <dbReference type="SMR" id="Q3YS40"/>
    <dbReference type="STRING" id="269484.Ecaj_0422"/>
    <dbReference type="EnsemblBacteria" id="AAZ68465">
        <property type="protein sequence ID" value="AAZ68465"/>
        <property type="gene ID" value="Ecaj_0422"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0422"/>
    <dbReference type="eggNOG" id="COG1005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_0_1_5"/>
    <dbReference type="OMA" id="WSGWASN"/>
    <dbReference type="OrthoDB" id="1559067at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01350">
        <property type="entry name" value="NDH1_NuoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018086">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432">
        <property type="entry name" value="PTHR11432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00667">
        <property type="entry name" value="COMPLEX1_ND1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00668">
        <property type="entry name" value="COMPLEX1_ND1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000244913" description="NADH-quinone oxidoreductase subunit H">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01350"/>
        </source>
    </evidence>
    <sequence length="369" mass="41223" checksum="D561BAA7F554F159" modified="2005-09-27" version="1">MSYYYNIFTDFISCISSGAVLLIKIMAVIISVMVSVAYLVYMERKVIGAIQLRQGPNVVGPFGLLQPFADAVKLIIKEHIIPFKSNKICFLIAPIITFTLALLGWAVIPFGADVIVNNGHEVIIPNVIANINIGVLYILAISSLGVYGIIIAGWSSNSNYAFLGAIRSASQMISYEVSIGLTIVTVLLATGSLKLGEIVVARHNMPYWIDLLLLPMACVFFISSLAETNRHPFDLPEAESELVSGYNVEYSSMPFALFFLGEYANMILINAMAVIFFFGGWYPPLNISFLYVIPGIIWFVLKIIVLLFCFIWIRATIPRYRYDQLMRLGWKVFLPISLLWVVLVSGVLLYTDSLPRNAYGIQYVHDLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS49</accession>
    <name>Q3YS49_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="8">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">lysS</name>
        <name evidence="9" type="ordered locus">Ecaj_0413</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304534.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0413"/>
    <dbReference type="EnsemblBacteria" id="AAZ68456">
        <property type="protein sequence ID" value="AAZ68456"/>
        <property type="gene ID" value="Ecaj_0413"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0413"/>
    <dbReference type="eggNOG" id="COG1384">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025562_2_0_5"/>
    <dbReference type="OMA" id="DWPMRWA"/>
    <dbReference type="OrthoDB" id="256927at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00177">
        <property type="entry name" value="Lys_tRNA_synth_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002904">
        <property type="entry name" value="Lys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940">
        <property type="entry name" value="PTHR37940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01921">
        <property type="entry name" value="tRNA-synt_1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00467">
        <property type="entry name" value="lysS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="34"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="281"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="511" mass="58866" checksum="C630D791AC047CB5" modified="2005-09-27" version="1">MMEQYKFWPFQEAEKILKRFGEDTEIIFETGYGPSGLPHIGTFGEVLRTTAIMNALRKICPDIVTKIIVVSDDMDGLRKIPDNIPNKDMIAEHLCKPLTMIPDPFCACKSYGHHMNMLLCKFLDLFNFQYEFKSATECYKNGLYDEKLLLLLKNYDKVMNVMLPTLGSERQKTYSPFLPVCPKTSKVLQVPIIDLDVELGTISYKNEDDEIIKTQVTGGKCKLQWKPDWGMRWSALNIKYEAYGKDLQPSAELSSKICEILGSAPPILFCYELFLDKEGRKISKSKGNGISIEDWLSYAPSESLALYILQNPKKAKKLYFDVIPRSMDDYLYLVDDYHKNPSYDNPVWHIHHGSVPIVDIFGLSFSLLLNLASACNAENSDVLWGFIKNYQDNHALQDNRLLSQLVDFSVKYYHDFVKPYKVYKTPDHDEKIILDELKNMLNDFPNDITSDEIQSNIFSLMKKYCFNDLRSCFKSFYEILLGQSHGPRLGSFIKLYGINNTINLIDQALKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS54</accession>
    <name>Q3YS54_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HesB/YadR/YfhF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0408"/>
    <dbReference type="EnsemblBacteria" id="AAZ68451">
        <property type="protein sequence ID" value="AAZ68451"/>
        <property type="gene ID" value="Ecaj_0408"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0408"/>
    <dbReference type="eggNOG" id="COG0316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069054_4_2_5"/>
    <dbReference type="OMA" id="DFAWRGL"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="FeS_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="FeS_cluster_insertion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00049">
        <property type="entry name" value="TIGR00049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S_biosyn" evidence="2">
        <location>
            <begin position="32"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="141" mass="16234" checksum="5585B60DDCCBDD60" modified="2005-09-27" version="1">MNNEISISYNNDGNSSQIRYYINSEVTSKSPIIITENAIKKIRQLIKQKEDAVIGIRIMVKQKGCFGFKYNIEYAYDIKLLDVKIEVEYQHENFTILIDPKAMMFINGTEIDHVENKISSGFTFKNPNEKGRCGCGESFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS62</accession>
    <name>Q3YS62_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304521.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0400"/>
    <dbReference type="EnsemblBacteria" id="AAZ68443">
        <property type="protein sequence ID" value="AAZ68443"/>
        <property type="gene ID" value="Ecaj_0400"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0400"/>
    <dbReference type="HOGENOM" id="CLU_195742_0_0_5"/>
    <dbReference type="OMA" id="KSYTHAF"/>
    <dbReference type="OrthoDB" id="1979961at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="76" mass="9107" checksum="DC0350C28EACB097" modified="2005-09-27" version="1">MEDPGTMIKCTLSYLNNTKSYTSAFKKNVIEAFEARLITEEQFTYMIHHVTKFIKKIEVYENMFLDIYDKHFISKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS77</accession>
    <name>Q3YS77_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA polymerase, sigma 32 subunit, RpoH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0385</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0385"/>
    <dbReference type="EnsemblBacteria" id="AAZ68428">
        <property type="protein sequence ID" value="AAZ68428"/>
        <property type="gene ID" value="Ecaj_0385"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0385"/>
    <dbReference type="eggNOG" id="COG0568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_3_5_5"/>
    <dbReference type="OMA" id="YQRWLAE"/>
    <dbReference type="OrthoDB" id="1565734at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="57"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="6">
        <location>
            <begin position="235"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="300" mass="35354" checksum="C0EF4EFD58D27421" modified="2005-09-27" version="1">MVSYINMLTNSIFSLTQDNLMSYMNEVHTFPILSPEEEDRLAKNWYENGIVEDAHRLVTSHLRLVVKIALSFKNYGLPLIELIMEGNIGLMQAVKKFNPTLGFRLSTYAIWWIKASIKDYILKSWSCIKIGTTQAQRKLFFSLRKIKQKLFKYNNITQDDIKLIANECSTSEQEVEQMNRYFTYRDRSLNEVVFSNDNQKGIELQEIIKCDTPNQEDTYLLNEEASLKETLITQALSTLNERYRDIFIRRRLIENPDTLDTLSQEYKISKERVRQIEMHAFTKVKNFIMLKKEELGLLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS93</accession>
    <name>Q3YS93_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304490.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0369"/>
    <dbReference type="EnsemblBacteria" id="AAZ68412">
        <property type="protein sequence ID" value="AAZ68412"/>
        <property type="gene ID" value="Ecaj_0369"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0369"/>
    <dbReference type="HOGENOM" id="CLU_1203285_0_0_5"/>
    <dbReference type="OMA" id="CIGYNYL"/>
    <dbReference type="OrthoDB" id="1797181at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="230" mass="26884" checksum="14B64CE55F32700C" modified="2005-09-27" version="1">MKNLKYIIVYFVVAIVAFDAEAIPFYIKGGCNFGVNYSDSLKSKYVNYERLNIDINTAIGYKLKNNFFYEFEVRYARIKPAIQELVDFEKMDLVDILQRIIGKKTYVSKIENIFEINNVTALINFGYDYIINDKLTAYLSYGVGIGGLFNYQGFRSNTATHYGISMQNEIGLCYTYNKKMSLCIGYNYLKNYWKYDTDKIYDEDGNSVIYHFRDFQLKSHTVFANFKVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS96</accession>
    <name>Q3YS96_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304487.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68409">
        <property type="protein sequence ID" value="AAZ68409"/>
        <property type="gene ID" value="Ecaj_0366"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0366"/>
    <dbReference type="HOGENOM" id="CLU_079587_0_0_5"/>
    <dbReference type="OMA" id="KSHASHG"/>
    <dbReference type="OrthoDB" id="1579188at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="275" mass="30952" checksum="26A83B345F3BC889" modified="2005-09-27" version="1">MKNDKTNLFNKKLIHKWQMAYICMSVFYLMLLSSAFFVMLYAPNDVKFIANYLLLGVACISFIAQLINITCNILNIVNIKREQKRNSKVATEARKELTVAVGFIGESIALLGIAVVVIMLGAQWLSFGAVALPLRILNIFASTFSIVYGSILLCKSIKTNSESKDSPNFNFRFHAKWVLVNSILFISLGFYNLIYETILHEVLDFTNNHGLAMLLKATVFLGYVCVLSSIFFSQLFDMHATMDKDAVIRCKIGYSSEEAEGLLKIQDCQGLKFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSA6</accession>
    <name>Q3YSA6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0356</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304477.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68399">
        <property type="protein sequence ID" value="AAZ68399"/>
        <property type="gene ID" value="Ecaj_0356"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0356"/>
    <dbReference type="HOGENOM" id="CLU_2507408_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="85" mass="9386" checksum="27126B9011D68C9C" modified="2005-09-27" version="1">MCKKEQELCLISSEQLSDKIPVSGKYLAARKEYNNDLSNLGKNVSSLLEDVNDDVDILQQQCFGEVSSLLESASACSPVMRSNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSB9</accession>
    <name>Q3YSB9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68386">
        <property type="protein sequence ID" value="AAZ68386"/>
        <property type="gene ID" value="Ecaj_0343"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0343"/>
    <dbReference type="HOGENOM" id="CLU_1406826_0_0_5"/>
    <dbReference type="OMA" id="NTIADCT"/>
    <dbReference type="OrthoDB" id="2121782at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="181" mass="20111" checksum="CF90CD2A1CC9FA09" modified="2005-09-27" version="1">MGMSRSIMIANAATIVLLALIGLIVSCVNFTFQSQSYVMTSIFSLLLIMGFTLLCVNCVAAKYDNVINVLYNNTVASDLDISKKEKTGIFAKLKNLLFRERSSQELETSSYSDQHDDSDHNSVIQVDGQRVLYNSGSEIDDSVEIDQNVSENSGSAECLDNTDFTIEDAEKMIQSRYFKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSC7</accession>
    <name>Q3YSC7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase M22, glycoprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304456.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0335"/>
    <dbReference type="EnsemblBacteria" id="AAZ68378">
        <property type="protein sequence ID" value="AAZ68378"/>
        <property type="gene ID" value="Ecaj_0335"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0335"/>
    <dbReference type="eggNOG" id="COG1214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064886_3_0_5"/>
    <dbReference type="OMA" id="NTSGPIC"/>
    <dbReference type="OrthoDB" id="1826274at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022496">
        <property type="entry name" value="T6A_TsaB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF11">
        <property type="entry name" value="PTHR11735:SF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03725">
        <property type="entry name" value="T6A_YeaZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TsaD" evidence="1">
        <location>
            <begin position="31"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="192" mass="21100" checksum="544DCAA6EE691F53" modified="2005-09-27" version="1">MAIMTINTSGPICSVATISENQVIHYEESTSHNQHSESLFKLIHTLFDKSKNSYSTISDIGVVVGPGSFTGIRVGIAAAQGIHIASNITLHGISNLEMQAYLIAQTTKNENILSVIEVSNNKFYYQTFNHQLIPTSKIQLLDKDIKCNNSKHNTQSITAPDFNAKTAALILAYKLQNNLPTLSTTPIYFNTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSD1</accession>
    <name>Q3YSD1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin reductase</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0331</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304452.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0331"/>
    <dbReference type="EnsemblBacteria" id="AAZ68374">
        <property type="protein sequence ID" value="AAZ68374"/>
        <property type="gene ID" value="Ecaj_0331"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0331"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_1_5"/>
    <dbReference type="OMA" id="VDNFPGY"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="5">
        <location>
            <begin position="8"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="321" mass="34554" checksum="77CCF061F2CE5304" modified="2005-09-27" version="1">MIHTYKTKVLIIGSGAAGCTAAIYAARANLEPILITGMCPGGQLTITTDVENFPGFAHAVQGPDLMEQMKQQAHNSGANVISDEIKEIHTDVYPFKCIGIFGDQYIAESIIIATGAQAKWLNIKSEETFKGRGVSACATCDGTFFTGSDVAVIGGGNTAVEEALYLTRHATKVFLVHRRDKLRAEPIMQERLLNNNKIQVIWDSVVEEILGNDENGTVEAVKLKSVKTGQFTTIKVKGVFIAIGHTPNTQILKNKDNSSIVELDQEGYIITKHGSTITSHPGIFAAGDVQDKIYRQAVVAAGSGCMAALEAAKFLSENTMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSD9</accession>
    <name>Q3YSD9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="16">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="16">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="16">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="16">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="15" type="primary">nnrE</name>
        <name evidence="14" type="synonym">nnrD</name>
        <name evidence="18" type="ordered locus">Ecaj_0322</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 16">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 15 16">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 14 16">
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 14 16">
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="15 16">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 16">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="16"/>
    <dbReference type="EC" id="5.1.99.6" evidence="16"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304444.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0322"/>
    <dbReference type="EnsemblBacteria" id="AAZ68366">
        <property type="protein sequence ID" value="AAZ68366"/>
        <property type="gene ID" value="Ecaj_0322"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0322"/>
    <dbReference type="eggNOG" id="COG0062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_4_1_5"/>
    <dbReference type="OMA" id="ALECEIT"/>
    <dbReference type="OrthoDB" id="1748633at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NADHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052857">
        <property type="term" value="F:NADPHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="SSF64153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="18" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 14 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14 16" id="KW-0520">NAD</keyword>
    <keyword evidence="9 14 16" id="KW-0521">NADP</keyword>
    <keyword evidence="7 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15 16" id="KW-0630">Potassium</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="17">
        <location>
            <begin position="4"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX" evidence="15">
        <location>
            <begin position="52"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX" evidence="15">
        <location>
            <begin position="109"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="378"/>
            <end position="382"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR017184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AAZ68366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="466" mass="50022" checksum="65EC6BEBB9B0E82E" modified="2005-09-27" version="1">MVVLNSQQVISYEKSCGIVVDELICRAGKAITDSISKLFPKQFVTVIAGPGNNGKDGVTAARMLKAQGWPVILMLYNCNVDIDEDWVIPLTYNSIVNCQSCLIIDAIFGIGLSRNIPEDLSCIFKYINNDSNNVIVAVDIPSGINCDTGQIMGCAIHADVTITFSVLKIGHILFPGCDYSGKVHVVDIGINIDYSRVAIRNNTPVLWKHEIPKLDYTVNKYKRGYTLVCSVGNKSIGASKLVAMAALRTGSGIVSIACDSNAVAFYASCLTSIMYKLYDDVINDDRVTSVVIGPGCGISDITKQRTVDVLSKKNCVLDADSISVFADSCETLFSEIKHNVIMTPHEGEFKRIFPFLTGSKIEVVQKAANLSKAVIVLKGPDTVIADPIGNVVVNNAPFNLATAGSGDVLSGIIGGLLSCGMTPLSAACCGVWIHAECARNYGIGLIADDIILQIPQILGKLFYSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSE9</accession>
    <name>Q3YSE9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0312</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304434.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0312"/>
    <dbReference type="EnsemblBacteria" id="AAZ68356">
        <property type="protein sequence ID" value="AAZ68356"/>
        <property type="gene ID" value="Ecaj_0312"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0312"/>
    <dbReference type="HOGENOM" id="CLU_1439020_0_0_5"/>
    <dbReference type="OMA" id="ETHNMTH"/>
    <dbReference type="OrthoDB" id="1770846at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="188" mass="21412" checksum="35D24258C59D147D" modified="2005-09-27" version="1">MMSSIFQKQLNVQFLNYIGDGICSLHSMLAAIKDKCNAHSSPDVNISFTIQHKDNVRNLIDQEYGHNTVTNLLHTCNNSNITLIDASEQPITNEEILSQDSRYSYNTIPQNDVTQYDEEISYETAYHLLLNLVNNDVNLFEEVISIAEVLVREGVLVAPESKIKKNIAQIILHNIQHKQKTQHSGYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSF8</accession>
    <name>Q3YSF8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal large subunit pseudouridine synthase C</fullName>
            <ecNumber evidence="4">5.4.99.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">23S rRNA pseudouridine(955/2504/2580) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">rRNA pseudouridylate synthase C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">rRNA-uridine isomerase C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0303</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Responsible for synthesis of pseudouridine from uracil at positions 955, 2504 and 2580 in 23S ribosomal RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(955/2504/2580) in 23S rRNA = pseudouridine(955/2504/2580) in 23S rRNA</text>
            <dbReference type="Rhea" id="RHEA:42528"/>
            <dbReference type="Rhea" id="RHEA-COMP:10099"/>
            <dbReference type="Rhea" id="RHEA-COMP:10100"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.24" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304425.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0303"/>
    <dbReference type="EnsemblBacteria" id="AAZ68347">
        <property type="protein sequence ID" value="AAZ68347"/>
        <property type="gene ID" value="Ecaj_0303"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0303"/>
    <dbReference type="eggNOG" id="COG0564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_1_2_5"/>
    <dbReference type="OMA" id="PKTHVYR"/>
    <dbReference type="OrthoDB" id="1714699at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="9">
        <location>
            <begin position="15"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="308" mass="35212" checksum="E5EEE5B5EF1B7CE8" modified="2005-09-27" version="1">MVGKYYTVKEDDQDVRVDKFVRRVLKIPQSLVEKFLRKGIVLLDNAKVKSNTRIKTGNVIYIKCNTIIGEEIHTKRICKTDSSYLINLISNSILYEDDDVLIINKPAGVSVQGGTKVRISISDILDKIRDGESMRIVHRLDKDTSGILMLARNARASRLIMHEFKNRRVEKKYLALTQGIPKNDVGEINHSITKKKHNFYENIGTGNSIFQEAITSFFVLKKLPYDIGLLELKPKTGRKHQIRIHLSHIGCSIIGDKKYGKFCNHVLNDHLQLHAHFLSIDIGNKKLSFTAPMPDYMKDTIMNLEKLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSH3</accession>
    <name>SURE_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="1">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">surE</name>
        <name type="ordered locus">Ecaj_0285</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304410.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSH3"/>
    <dbReference type="SMR" id="Q3YSH3"/>
    <dbReference type="STRING" id="269484.Ecaj_0285"/>
    <dbReference type="EnsemblBacteria" id="AAZ68332">
        <property type="protein sequence ID" value="AAZ68332"/>
        <property type="gene ID" value="Ecaj_0285"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0285"/>
    <dbReference type="eggNOG" id="COG0496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045192_1_2_5"/>
    <dbReference type="OMA" id="MHVEERI"/>
    <dbReference type="OrthoDB" id="1909278at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106411">
        <property type="term" value="F:XMP 5'-nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="PTHR30457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SSF64167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000235613" description="5'-nucleotidase SurE">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <sequence length="256" mass="27714" checksum="7CE7A8CD3A7EED22" modified="2005-09-27" version="1">MKVLLTNDDGFHATGIKVLKEIIIESGIASEVWIAAPASNCSGYGRSIGVKTATDVYQIGDTEFIVNSTPSTSAFLGLREITGKKPDLVLSGINSGINVGNDIGYSGTIGAAAEGAMMNIPSIAISQEYDGKHGEINWINPQKFLKKIINMLLEVPFWNKSTIMNVNFPLVPAKGIKFTSQGEYIPSNKIEKKRNVSGSISYTVHRIAPDKGNRGYSDDSIKAIDDGYITITPLKLDMTDFDALESLIALYKDCTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSH9</accession>
    <name>Q3YSH9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Smr protein/MutS2 C-terminal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304404.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0279"/>
    <dbReference type="EnsemblBacteria" id="AAZ68326">
        <property type="protein sequence ID" value="AAZ68326"/>
        <property type="gene ID" value="Ecaj_0279"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0279"/>
    <dbReference type="eggNOG" id="COG2840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055978_2_1_5"/>
    <dbReference type="OMA" id="MQEEAYF"/>
    <dbReference type="OrthoDB" id="1888882at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SSF160443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="1">
        <location>
            <begin position="112"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="204" mass="23239" checksum="233D672FCE68605C" modified="2005-09-27" version="1">MLKKSKRETPNSSSCDDIVSWDSITLIVTPIYNNKVPYKKHIDNNQYCISSNDRHTNHLIPIQTNSNITYNTGISSIQTCSNSTYDISSGLNLNNHTKKKIDKGKYYIDATLDLHGYTLDSAYDKLLDFIVKNYNLSNKCLLIITGWGSTNKNNSNSIKSNFSKWLQNEQIAKIILYYREAINSHGGKGAFYVLLKSKNKKRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSI3</accession>
    <name>Q3YSI3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304400.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0275"/>
    <dbReference type="EnsemblBacteria" id="AAZ68322">
        <property type="protein sequence ID" value="AAZ68322"/>
        <property type="gene ID" value="Ecaj_0275"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0275"/>
    <dbReference type="eggNOG" id="COG0470">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_983347_0_0_5"/>
    <dbReference type="OMA" id="SWLICGK"/>
    <dbReference type="OrthoDB" id="1971388at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="19"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="296" mass="33835" checksum="38A5BD02AFD41BF9" modified="2005-09-27" version="1">MNKIIGHTLAKNTLINNTHIQSWLLYGNKGIGKATLAYNFAQYLTQSPNSNYCNPDLLIINDNEEIIGIEKIRNIKNFLYLTTARFKYRIVIIDSIDSLTINAINAMLKILEETPTNSIIILISHNLHEVPVVIRSRCFTLGLSDLNPKQTQQVIKNNFPHLDYEKVSHIYPGTPGMVDQHIEKEIALHESLISILTHQYDSKIIENIITTDISFYKIEYILLTIVSNLAKEILGITTSLSNYNTLVDNIKNKKVDVDKLLSKLLQIQHLISYAKKFQLDKKSVMLNLVNIMIYLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSJ8</accession>
    <name>MUTS_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA mismatch repair protein MutS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mutS</name>
        <name type="ordered locus">Ecaj_0260</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304385.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSJ8"/>
    <dbReference type="SMR" id="Q3YSJ8"/>
    <dbReference type="STRING" id="269484.Ecaj_0260"/>
    <dbReference type="EnsemblBacteria" id="AAZ68307">
        <property type="protein sequence ID" value="AAZ68307"/>
        <property type="gene ID" value="Ecaj_0260"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0260"/>
    <dbReference type="eggNOG" id="COG0249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002472_4_0_5"/>
    <dbReference type="OMA" id="TPMMAQY"/>
    <dbReference type="OrthoDB" id="15991at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00096">
        <property type="entry name" value="MutS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005748">
        <property type="entry name" value="DNA_mismatch_repair_MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="PTHR11361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="SSF48334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="SSF53150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="SSF55271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01070">
        <property type="entry name" value="mutS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224369" description="DNA mismatch repair protein MutS">
        <location>
            <begin position="1"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="614"/>
            <end position="621"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00096"/>
        </source>
    </evidence>
    <sequence length="804" mass="91004" checksum="896D36BBEA882935" modified="2005-09-27" version="1">MNHDRKITPIMQQYMMLKNQYKECLLFYRLGDFYELFFDDAIETSKVLNIVLTKKGNVPMCGVPFHSSESYLNRLVKLGYKVAICEQLETPEEAKKRGYKALVKRDVVRIVTPGTILEDSLLEAKENNYLCCIVNVNDNYAIAWLELSTGLFHYNTTDLRKIDSDLLRINPKEVLISDKLVELDLIYSTLKKYKFSITQYSSSFFDTSRSYNTLCSVYGISTLKGLGDLKDEEIAVCGSLLEYVKATQKGNLPKLEFPKAYGDRDFMFIDAAALRNLELFNTQSGDLEGSLISSIDYTITACGGRLLKRCLSAPLACSYAINRRLDIVEFFVNNKVLYKNVRETLRGIADIERILTRVKVGRCSPKDLYALKLTLDKTCILVNLLSKFDTSVVSDFCTRLGRYDDLCKTLSNVLIPNNVSNVKDGGFIKPDYDVQLSEYIYIQNYSNNLIQELRDKYRNITNIQNLKILYNNILGYYVEVSANHLINDKDFIHRQTLANSIRYTTSELKALESKIISARDAAISLEVKIFGQLCTRIIEHADEITMTAHAVSEIDMLTSFAELAIQYSYTRPVVDDSYEFNIQKGRHPVVERNGKFVANDIDLAQMQRVHLITGPNMAGKSTFLRQNALIGILAHIGSFVPAYSAHIGVIDKIFSRVGASDNIAFGYSTFMVEMTETAAIINQATDKSFVILDEIGRGTGTYDGLSIAWSVIEQIHNVNKSRAIFATHYHELSKLDECLENIKCFCMKVEEWNGKVVFLHEIIPGSTNKSYGIHVAKLAGFPQSVLDRAEDLMNKLKANEDLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSK2</accession>
    <name>Q3YSK2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage major capsid protein, HK97 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0256</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304381.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0256"/>
    <dbReference type="EnsemblBacteria" id="AAZ68303">
        <property type="protein sequence ID" value="AAZ68303"/>
        <property type="gene ID" value="Ecaj_0256"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0256"/>
    <dbReference type="eggNOG" id="COG4653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041417_0_0_5"/>
    <dbReference type="OMA" id="PYRNKAV"/>
    <dbReference type="OrthoDB" id="755947at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024455">
        <property type="entry name" value="Phage_capsid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05065">
        <property type="entry name" value="Phage_capsid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01554">
        <property type="entry name" value="major_cap_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="382" mass="43614" checksum="78C9C5B2AA576CF2" modified="2005-09-27" version="1">MTTSTLEIQESLNNLTASWEEFKTQNEKKLSDISAKSAIYPSILEKLEKTENILDIQNQRLDQLEISSQRPITYDNKHYNEDHNYKMFTQYLCKGIDEIKSATEEALDESYSVPHNISSYIEKNLTQNSVMRQLCSIEKISGDSLDCFISDNNQTNVNWRTSSQDTDETKPPKIDKVTINLHELYAQPKITKKLLEDSTIDIASWLINHLVDAFSRAENTAFISGDGNNKPYGILTYAKEENTIESKTLNSDTIIKLYYSLDEYFATKAAFIMHRSVLEAIRSLKLTSGQYIWHPGLTPGNPDTLMGLPVYQTSDMPQLNKKSLPIIALADFKNAYKIVENRSIRTLRDPFTSKPFVKFYTTKRVGGAVINDNAIRFLKITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSP5</accession>
    <name>Q3YSP5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YggT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304338.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0211"/>
    <dbReference type="EnsemblBacteria" id="AAZ68260">
        <property type="protein sequence ID" value="AAZ68260"/>
        <property type="gene ID" value="Ecaj_0211"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0211"/>
    <dbReference type="eggNOG" id="COG0762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136788_0_1_5"/>
    <dbReference type="OMA" id="WLINFQV"/>
    <dbReference type="OrthoDB" id="2069856at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003425">
        <property type="entry name" value="CCB3/YggT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02325">
        <property type="entry name" value="YGGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="94" mass="11232" checksum="5ACEC8280E5A5D13" modified="2005-09-27" version="1">MHPLVYLLDTLLSIYNFSLVLWIILSWLIFLNIVNRYNEIVSNVFLLLSKLMSPALNFVRKIFPFTVSYNFDLSPLILLIFINFVRYALRYYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSS2</accession>
    <name>Q3YSS2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Patatin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0182</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304311.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68233">
        <property type="protein sequence ID" value="AAZ68233"/>
        <property type="gene ID" value="Ecaj_0182"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0182"/>
    <dbReference type="eggNOG" id="COG3621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_144_9_5"/>
    <dbReference type="OMA" id="PTYFEPA"/>
    <dbReference type="OrthoDB" id="373926at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="6"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="2">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="42"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="315" mass="34630" checksum="323F1421A67F32F2" modified="2005-09-27" version="1">MTKYVLSVDGGGVRGIVAATILQEIEKRINKPLCKVFDLVSGSSVGSLICGALCVKNADGTPRYSACDLLELILMYAGKIFCNSTVRNALSLVFGPKYSDKNLNAVLQEIFGDVTIKDLIADFIVPSYDLCSNQTIMFRSWIDKYSDIKVCDVTRAAVAAPTYFTPKKMIVDNKKQLLVDSAIVCNNPVIAAYSAAQVLYPNEKICCLSVGCGTVSQSFSDLQNSLLYWSRKILCVIIDAGLEAIDYEMARLVKGEDSYCRISGDIVYSACDFGDASQENIKNLKKDAQKILQENEKNINDFCDMLLSDDTIKNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSS7</accession>
    <name>COAX_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type III pantothenate kinase</fullName>
            <ecNumber evidence="1">2.7.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PanK-III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pantothenic acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaX</name>
        <name type="ordered locus">Ecaj_0177</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NH4(+)</name>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
        </cofactor>
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">A monovalent cation. Ammonium or potassium.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type III pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304306.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSS7"/>
    <dbReference type="SMR" id="Q3YSS7"/>
    <dbReference type="STRING" id="269484.Ecaj_0177"/>
    <dbReference type="EnsemblBacteria" id="AAZ68228">
        <property type="protein sequence ID" value="AAZ68228"/>
        <property type="gene ID" value="Ecaj_0177"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0177"/>
    <dbReference type="eggNOG" id="COG1521">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066627_1_0_5"/>
    <dbReference type="OMA" id="HEPWLTL"/>
    <dbReference type="OrthoDB" id="2039419at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00352"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01274">
        <property type="entry name" value="Pantothen_kinase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004619">
        <property type="entry name" value="Type_III_PanK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34265">
        <property type="entry name" value="PTHR34265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03309">
        <property type="entry name" value="Pan_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00671">
        <property type="entry name" value="baf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000267525" description="Type III pantothenate kinase">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="6"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="108"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01274"/>
        </source>
    </evidence>
    <sequence length="264" mass="29574" checksum="AEA48DC1AF516D42" modified="2005-09-27" version="1">MLIAIDVGNTNIKFAICTSNRILKIIRIASQQAKTADQYFFYLNNIINQLNTDFKNINNIIISSVVPSITKPMLELSKNYFNINPIILDNNHADIFNIKINLQNKALGSDRLADIIAASNIYPNKDLLIIGMGTATVFNLLNKDKCIYGQVIAPGAHILAKSMRQFTALLPEVSVSKQDKVVHNNIYHAMESGVYWGYITMVNGMIEKIIKEEKKDLHVIATGGNSYLFFDHKTAINNIETDLTIKGILYLHNMLSNNTAQNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YST2</accession>
    <name>Q3YST2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304301.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0172"/>
    <dbReference type="EnsemblBacteria" id="AAZ68223">
        <property type="protein sequence ID" value="AAZ68223"/>
        <property type="gene ID" value="Ecaj_0172"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0172"/>
    <dbReference type="eggNOG" id="COG1238">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153235_0_0_5"/>
    <dbReference type="OMA" id="WYLGRIT"/>
    <dbReference type="OrthoDB" id="1928533at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="138" mass="15775" checksum="81139746F5A02DA9" modified="2005-09-27" version="1">MIEIYSLLFIDSLVAALILPLNKVLIFKIMTYFGGYSYALMLLVSTLGTVVGGIINCILGRMIIFARVEYHKMQDDYGRLGYYIKLVLMLLTLLCSWIPVWGGIINVLSGYFKVRMLNLIVLLFISYLGYFIHCIMIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YST4</accession>
    <name>RPOC_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit beta'</fullName>
            <shortName evidence="1">RNAP subunit beta'</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit beta'</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit beta'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoC</name>
        <name type="ordered locus">Ecaj_0170</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304299.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YST4"/>
    <dbReference type="SMR" id="Q3YST4"/>
    <dbReference type="STRING" id="269484.Ecaj_0170"/>
    <dbReference type="PRIDE" id="Q3YST4"/>
    <dbReference type="EnsemblBacteria" id="AAZ68221">
        <property type="protein sequence ID" value="AAZ68221"/>
        <property type="gene ID" value="Ecaj_0170"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0170"/>
    <dbReference type="eggNOG" id="COG0086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_3_1_5"/>
    <dbReference type="OMA" id="YRNIRVE"/>
    <dbReference type="OrthoDB" id="4421at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01322">
        <property type="entry name" value="RNApol_bact_RpoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012754">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="PTHR19376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02386">
        <property type="entry name" value="rpoC_TIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000225533" description="DNA-directed RNA polymerase subunit beta'">
        <location>
            <begin position="1"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="810"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="884"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="891"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="894"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01322"/>
        </source>
    </evidence>
    <sequence length="1410" mass="157617" checksum="AEDA247B785BD035" modified="2005-09-27" version="1">MKMLDLYGYTSIAQSFDKICISIASPESIRAMSYGEIKDISTTNYRTFKVEKGGLFCPKIFGPVNDDECLCGKYRKKRYRGVICEKCGVEVTSSKVRRERMGHIELVSPVAHVWFLKSLPSRIGALLDMPLKLIESILYSGDFVVIDPIATPLSKGEVISESAYNQAKDNYGEDSFLALTGAEAIRELLVRLDLHAINANLRTELESTTSEMKRKKIVKRLRIVENFINSGNKPEWMILTVIPILPPDLRPLVSLENGRPAVSDLNHHYRTIINRNNRLGKLLKLNPPAIMIRNEKRMLQEAVDALFDSTRRSYVSNKAGSVGYKKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPNLKLHQCGLPKKMALELFKPFICSKLKMYGIVPTVKLANKMIQNEKPEVWDILDEVIHEHPILLNRAPTLHRLGIQAFDPVLIEGKAIQLHPLVCSAFNADFDGDQMAVHIPLSLEAQLEARILMMSTNNILSPSNGKPIIVPSKDIVLGIYYLTLQDQAQSEDEVLFFGDFSHVEYALHNKDVHLCSKIKYRMTYCNYDVEGNKPSYYSKIIETTPGRLILWQIFPQHKNLTFDLINQVLTVKEITSIVDLVYRNCGQSETVEFSDKLMSLGFKYASQSGISFGCKDMIIPDTKTMHVDNASDKIKEFAVQYQDGLITRSERYNKVIDEWSKCTDLIAKDMMKAISVYDEENKLNSIYMMAHSGARGSASQMKQLSGMRGLMAKPSGEIIETPIISNFREGLNVFEYFNSTHGARKGLADTALKTANSGYLTRRLVDVAQDCIVVEYDCKTHNGFAIRSVVEGGAVVETLDNIILGRVAAVDVYNPITEELLVKAGELIDEAKVEKIKIAGLDAVKVRSPLTCEAKKGICALCYGRDLAIGDVVSIGEAVGVIAAQSVGEPGTQLTMRTFHVGGTAMRGVETSNLIALLDAKAKLVNSNVVEDKHGNKIVMSRSCEIVLLDSVGNEKMRHNIPYGARLYVNDGQFVKITEKIAEWDPYTMPIITEKTGIIKYMDLIDGVSINEVLDESTGISNRVVVDWKLHLQGANLRPRLVLVDDNGNIITLSNGLEANYFIPIGAVLNVQDGQKVHAGDVITRIPRESIKTRDITGGLPRVIELFEARRPKEHAIVSDIDGYVEFGKDYYRSKRRIFIKPVDDKLTPVEYLVPKGKHTIVNEGDFVHKGDLLMDGDPDPHDILRVLGVEALANYMISEIQQVYRLQGVRIDNKHIEIILRQMLQKVEIFEPGDTMYLVGENIDMEEVIKTNNSMVKMGKSPAKYAPILQGITRASLDTNSFVSAASFQETTKVLTEAAFSGKEDSLYGLKENVIVGRLIPAGTGFLMNKIKKLSLLNKEDYSMYYDSEYQDPALLQSDSNNFGHDLPKGNNNIVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSW8</accession>
    <name>Q3YSW8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304265.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0136"/>
    <dbReference type="EnsemblBacteria" id="AAZ68187">
        <property type="protein sequence ID" value="AAZ68187"/>
        <property type="gene ID" value="Ecaj_0136"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0136"/>
    <dbReference type="eggNOG" id="COG3820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081814_1_0_5"/>
    <dbReference type="OMA" id="KATAMWL"/>
    <dbReference type="OrthoDB" id="1621803at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010421">
        <property type="entry name" value="TrcR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06242">
        <property type="entry name" value="DUF1013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="213" mass="23724" checksum="F1FEDCF68351C581" modified="2005-09-27" version="1">MSNSNVPLSPNPKGSSSTLEDSGPLMPRAVAVWLVDNTILTFEQIAKFCNLHLLEVKGIADGEVAKGVVGCNPITMGQVTKEEIECCQNDPSRIPRLISYRNFKKSKKRTVRYTPIARRRDKPDAVAWILKYCPEMQDVCIAKLIGTTKSTIAAIRNKEHWNSSNIKPRDPVLLGLCTQVDLDEAIMKAQIVAERERRVKNIKASDFSISDSV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSX1</accession>
    <name>SYFA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheS</name>
        <name type="ordered locus">Ecaj_0133</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304262.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSX1"/>
    <dbReference type="SMR" id="Q3YSX1"/>
    <dbReference type="STRING" id="269484.Ecaj_0133"/>
    <dbReference type="EnsemblBacteria" id="AAZ68184">
        <property type="protein sequence ID" value="AAZ68184"/>
        <property type="gene ID" value="Ecaj_0133"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0133"/>
    <dbReference type="eggNOG" id="COG0016">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025086_0_1_5"/>
    <dbReference type="OMA" id="DWHNFTA"/>
    <dbReference type="OrthoDB" id="469058at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00281">
        <property type="entry name" value="Phe_tRNA_synth_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004188">
        <property type="entry name" value="Phe-tRNA_ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022911">
        <property type="entry name" value="Phe_tRNA_ligase_alpha1_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PTHR11538:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02912">
        <property type="entry name" value="Phe_tRNA-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000231981" description="Phenylalanine--tRNA ligase alpha subunit">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with beta subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00281"/>
        </source>
    </evidence>
    <sequence length="345" mass="39884" checksum="25B39706DA021193" modified="2005-09-27" version="1">MLHSNIYSLQTEATNKILSTSSLEELESLRLYYFGKSGVITACLRSISTINNIEERKSIGSAVNSICAELKSLMNSQKEKLHKIQIDAQLMEDKIDISLPVRPKQIAKLHPISKTLHEVKHIFVSLGFKLSDGPELEDEFHVFDALNTHKHHPAREENDTFYLKKLVNNKRIVLRTHTSSVQIRTMESNNGNYPIKIIAPGKVYRNDWDATHSPMFHQIEGLYIDKNINMGHLKYCINYFLKKFFGENIQIRFRNSYFPFTEPSAEVDIKCSQKNWMEILGCGMVHHNVLTNVNINPEQYSGFAFGIGIERIAMIKYNINDLRKFYSNKLQWLTHHGFCFTNLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSY8</accession>
    <name>Q3YSY8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phage integrase, N-terminal SAM-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0116</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304245.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0116"/>
    <dbReference type="EnsemblBacteria" id="AAZ68167">
        <property type="protein sequence ID" value="AAZ68167"/>
        <property type="gene ID" value="Ecaj_0116"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0116"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_0_5"/>
    <dbReference type="OMA" id="HRFLYAE"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="107"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="309" mass="35467" checksum="2B18EDB48D65B627" modified="2005-09-27" version="1">MDNHKCVEIFLEAITAEKHISYNTYQSYKTDLLGLCEFVSKKNLLLVDTSINELQNYIRLLYEKGYKANTVLRKISAIKNFYGFLYRDNMIESDPTLYLDAPKLSRSLPKALSFEEISRLLDVAALDGSPDGRRLNAMVNILYSSGIRISELVCLKFYSVLEALKDHNAEIYHIKIKGKANKERIVLLNLSAVESIKKYIEIYKYFVPNGFEVSQWLFPGTKFDYPITRQRVGQLLKDLAMNAGIDITKISPHKLRHSFATHLLNNGSNIIFIQKMLGHANLSTTQIYTHIANEKLKNVLLKFHPLNNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSZ3</accession>
    <name>GRPE_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein GrpE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">HSP-70 cofactor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">grpE</name>
        <name type="ordered locus">Ecaj_0111</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304240.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSZ3"/>
    <dbReference type="SMR" id="Q3YSZ3"/>
    <dbReference type="STRING" id="269484.Ecaj_0111"/>
    <dbReference type="EnsemblBacteria" id="AAZ68162">
        <property type="protein sequence ID" value="AAZ68162"/>
        <property type="gene ID" value="Ecaj_0111"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0111"/>
    <dbReference type="eggNOG" id="COG0576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057217_6_2_5"/>
    <dbReference type="OMA" id="YAYEKIA"/>
    <dbReference type="OrthoDB" id="1906715at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_coiled_coil"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="PTHR21237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="SSF51064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="SSF58014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_1000053577" description="Protein GrpE">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="199" mass="22842" checksum="04277E94CDCDD65F" modified="2005-09-27" version="1">MSDSDNNTKSQQNNPTQTDEKSGEEIQSNQKPQRKFTAVLNKKKEKLNEDLSELDKLKQQLAHFQNQFRLAVADKENVKRIMQKNIDDTSIYAISNFARDLLSSCDNLETSLKNLKEDDSIHAGVLMTYKELLNTLERHNITRIDPIGEKFNPQFHKAVSQMTDEDKDENTILHVVQPGYIIKDKLLRPASVIISKKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT05</accession>
    <name>Q3YT05_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304228.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0099"/>
    <dbReference type="EnsemblBacteria" id="AAZ68150">
        <property type="protein sequence ID" value="AAZ68150"/>
        <property type="gene ID" value="Ecaj_0099"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0099"/>
    <dbReference type="HOGENOM" id="CLU_408682_0_0_5"/>
    <dbReference type="OMA" id="CTHIVTH"/>
    <dbReference type="OrthoDB" id="1678980at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="538"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="580"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="630" mass="71176" checksum="3B9B1B5AE8AD9FE5" modified="2005-09-27" version="1">MPTQEQQTSIINQKNSEIITSLAGLLFQEFSHNISHEPSNDKLTISYTLCQTAKDQPINIDNLSEAIVIFLEFYVVSNFILPYHPNSPDQEPSPPERSLLSFKIRINNKAESSEITPDKIITQSSSKILTITNPLKSVIRTSLEKEITKHCSITSNQPLTLCFITTFNTPFNIDTLNAHLTNNLATTQGILKLYPIIRLYNTSPITSTLPNCQKSLRLTVAHIKNPKNKGNKAQISMCLMTYNTEHTPVFTPLTHLCKCQYNHFSCALYEIDNTALGELPDLQDYPLHIQDNTYDLQTATYSLTYSSFTKAQLCQQLQYYASLFRDLYGEQHVKLSISFSLKENITPQPETLPKCYSQELNPETNIETLLNTACCIPEQSAFSKIFYKKYNEQTLEQILSQYTINLHLTCKDLLLEPSQDLPTSLPPVSTTTSHQVQSTHVIDPTTQQPDSQATSQITHRTQTSSQTQSSMLLPVTTPTAKTLSTTRSRTLRPRYKSTQLPTIPEVPEHTPKYKPVSSNNLQTFLTNKSKYKKRQKSSIMCTAIATAILTCLSIFGYNFLATHWKFLAEQQNIAKINQTVTLGILVFFGILFIGYVIYSQIKTSHDISENMINNPTIDPVEINQSIIDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT07</accession>
    <name>Q3YT07_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15 16">Protoporphyrinogen IX oxidase</fullName>
            <shortName evidence="15">PPO</shortName>
            <ecNumber evidence="15 16">1.3.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Ecaj_0097</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 16">Catalyzes the oxidation of protoporphyrinogen IX to protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>3 A + protoporphyrinogen IX = 3 AH2 + protoporphyrin IX</text>
            <dbReference type="Rhea" id="RHEA:62000"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 16">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 15 16">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cell membrane</location>
            <topology evidence="3 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15 16">Belongs to the HemJ family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="15 16"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304226.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0097"/>
    <dbReference type="EnsemblBacteria" id="AAZ68148">
        <property type="protein sequence ID" value="AAZ68148"/>
        <property type="gene ID" value="Ecaj_0097"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0097"/>
    <dbReference type="eggNOG" id="COG1981">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125006_1_0_5"/>
    <dbReference type="OMA" id="VRLFIYH"/>
    <dbReference type="OrthoDB" id="1433260at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070818">
        <property type="term" value="F:protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02239">
        <property type="entry name" value="HemJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005265">
        <property type="entry name" value="HemJ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40255">
        <property type="entry name" value="PTHR40255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03653">
        <property type="entry name" value="UPF0093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004638">
        <property type="entry name" value="UCP004638"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 15 16" id="KW-0349">Heme</keyword>
    <keyword evidence="13 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="14 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR004638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAZ68148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="144" mass="16869" checksum="DEA544D5821FFFA2" modified="2005-09-27" version="1">MIDYKHWIEAFHIISIITWMAGMLYLPRIYVYHAQVPLHSDSYNLFNTMESKLLFYIINPSMISSIALGTALSIITEAYYFLWFKIKIVCVILMCIIQVMLFKHRKDFAAKNNTKSSLYFKCLNESVTLLIIIIVIMVVVKPFM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-26" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT10</accession>
    <name>DAPE_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Succinyl-diaminopimelate desuccinylase</fullName>
            <shortName evidence="1">SDAP desuccinylase</shortName>
            <ecNumber evidence="1">3.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapE</name>
        <name type="ordered locus">Ecaj_0094</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-diaminoheptanedioate + succinate</text>
            <dbReference type="Rhea" id="RHEA:22608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:58087"/>
            <dbReference type="EC" id="3.5.1.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) or Co(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family. DapE subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAZ68145" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68145.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_044261945.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT10"/>
    <dbReference type="SMR" id="Q3YT10"/>
    <dbReference type="STRING" id="269484.Ecaj_0094"/>
    <dbReference type="EnsemblBacteria" id="AAZ68145">
        <property type="protein sequence ID" value="AAZ68145"/>
        <property type="gene ID" value="Ecaj_0094"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0094"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_4_0_5"/>
    <dbReference type="OrthoDB" id="275025at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00021"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009014">
        <property type="term" value="F:succinyl-diaminopimelate desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01690">
        <property type="entry name" value="DapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005941">
        <property type="entry name" value="DapE_proteobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01246">
        <property type="entry name" value="dapE_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000375544" description="Succinyl-diaminopimelate desuccinylase">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="380" mass="41738" checksum="27BF9BB13643DA10" modified="2009-05-26" version="2">MAIDPIKLSQELISFPSITPTDNGAIDFLSNTLSQYGFTCHVLTFGDDQVQVRNLYAQLGNGSPNLCFAGHTDVVPTGDSEKWKFDPFSGKIEDNILYGRGVVDMKSAICAFVAAVSRIDFNQINGSISFLISGDEEGNYFKYGTPAVLKWLTDNNKKIDFCLVGEPTSQSSVGDTIKIGRRGSINFKIVCNGVQGHVAYPHLAENPVDNMISILYKISNTVLDNGNEYFQPSNCEITSVDVGNTATNVIPDKITANLNIRYNDMHTSESLFNIINNICAEITEKYELFTAVSGSSFITHPGKHSDMLSSAIKKVTGQDAILSTSGGTSDARFIKDFCPVIELGLSNETAHKINEHAPVDDIYKLTDIYEEFIRQFFNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT15</accession>
    <name>Q3YT15_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycine cleavage T protein(Aminomethyl transferase)</fullName>
            <ecNumber evidence="5">2.1.2.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0089</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.2.10" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304218.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0089"/>
    <dbReference type="EnsemblBacteria" id="AAZ68140">
        <property type="protein sequence ID" value="AAZ68140"/>
        <property type="gene ID" value="Ecaj_0089"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0089"/>
    <dbReference type="eggNOG" id="COG0354">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_7_1_5"/>
    <dbReference type="OMA" id="MDRLHGV"/>
    <dbReference type="OrthoDB" id="1984573at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCV_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045179">
        <property type="entry name" value="YgfZ/GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GcvT_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602">
        <property type="entry name" value="PTHR22602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="GCV_T" evidence="4">
        <location>
            <begin position="13"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="278" mass="31911" checksum="428AA9EC61ED95FE" modified="2005-09-27" version="1">MSKFIVLPNRSIVLFHGPDARQLLNRTTTNNVLNLTQNKAVYSLLLSPSGRYMYDFFVVQYEKYILLDCCSIDKDEIIQKFLSYKLQSKVVIREKKHYKVGVFIGEESSSNVCGYTYCEGNTIFFQDPRLSTLGLRVIFDESNEALSNVNSDAERYKDYEMLRINNTVPDCNKDMIKGTSFPLQFRMDEFNAIDFNKGCYIGQEVVARMYRAGVKKKIYTVISESESFDDTKVMWDQKQVGELLSNVGNIGLCLLDINSCDNLCDLKIGKAKIKVLFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT31</accession>
    <name>Q3YT31_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0073</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304202.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68124">
        <property type="protein sequence ID" value="AAZ68124"/>
        <property type="gene ID" value="Ecaj_0073"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0073"/>
    <dbReference type="HOGENOM" id="CLU_161893_0_0_5"/>
    <dbReference type="OMA" id="IEIMMPH"/>
    <dbReference type="OrthoDB" id="1873884at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="92" mass="10358" checksum="C71A3B0F2E31627F" modified="2005-09-27" version="1">MTEELSLDSSLESAMSYVEKGYNTKIDSKMKECIKNEVKQAIPELNKVLTPLINSAISSEQQTLSQNQQADLVKKLIEIMMPHMQKIITASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT33</accession>
    <name>Q3YT33_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304200.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0071"/>
    <dbReference type="EnsemblBacteria" id="AAZ68122">
        <property type="protein sequence ID" value="AAZ68122"/>
        <property type="gene ID" value="Ecaj_0071"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0071"/>
    <dbReference type="HOGENOM" id="CLU_426840_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021902">
        <property type="entry name" value="DUF3514"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12027">
        <property type="entry name" value="DUF3514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="641" mass="71360" checksum="261201085C84BAA8" modified="2005-09-27" version="1">MQSDTHESIVRVDVSNGSNDEQLNDNSEFAKRTDKSTQKTKGSDRKSECLLKDIVESSEKTLVTESTSAYSQDIHDAEEFESRLSSSQWSTVQKGTRSTTKGDKEQKKFSGDGVKCLGKTEGVYSESVVESSAQRVSVPKGSIVENSSQWDKKNQDIKNSQLKLLKAENIAESGIISGSGEEWSVKFSKKDSKRNKKSQTFESDATVKAIDVSHASTVGEGKASQELSSKYLQQGARPKVRSHTQSISVGLPIGNANSKDVSQKKNVSLSTVNVSEMKAHSCMVTGIGTGVHLETKPIGRSGDTYGCVTNKQYLASVDKFKMSVEKFAKMHMQYIANIVFDVFAIENGNIIFRDEIQNVLLKTTKNIELCSFVIKQGIIMQVMSRVCSSKLYNSVMYCASHWDNQKILDAVLLEILNLGPNALVSMLPSMRNVFLNMHEGIEHNVFRLYSGGFYSGLLNMCYDFITQIDKSPGLMRLYNFIILSSQIHFGTMYDVICKSQVTAAGVQNVEAIKKLIFNGHRVGSVQICPTIFVHRCCGVKRTFRHLYSPNYKNVACTIGNLNIPSVIIKECSCNIDEKVAQCIEEKTMSFDVFVEDMVCNCVKSLLYQNVKLLLEKDIALYEKEVRSKYQTMGSTTSSRML</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT34</accession>
    <name>Q3YT34_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304199.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68121">
        <property type="protein sequence ID" value="AAZ68121"/>
        <property type="gene ID" value="Ecaj_0070"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0070"/>
    <dbReference type="HOGENOM" id="CLU_2616383_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="78" mass="7985" checksum="E1CADE58F7BB4351" modified="2005-09-27" version="1">MIFPAIAVATVATTLTIVVLCDKKSGKIGNKSNIDNIEASTSSSTATTSDEELAEELSGLDIASSKSRILDRGGGELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT56</accession>
    <name>Q3YT56_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">DNA polymerase I</fullName>
            <ecNumber evidence="4 11">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">polA</name>
        <name evidence="14" type="ordered locus">Ecaj_0048</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Single-chain monomer with multiple functions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="4 11"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304177.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0048"/>
    <dbReference type="EnsemblBacteria" id="AAZ68099">
        <property type="protein sequence ID" value="AAZ68099"/>
        <property type="gene ID" value="Ecaj_0048"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0048"/>
    <dbReference type="eggNOG" id="COG0749">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004675_0_0_5"/>
    <dbReference type="OMA" id="NRPPMPD"/>
    <dbReference type="OrthoDB" id="1220182at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019760">
        <property type="entry name" value="DNA-dir_DNA_pol_A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018320">
        <property type="entry name" value="DNA_polymerase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="PTHR10133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00593">
        <property type="entry name" value="pola"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00447">
        <property type="entry name" value="DNA_POLYMERASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="7 11 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="53EXOc" evidence="12">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="POLAc" evidence="13">
        <location>
            <begin position="618"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAZ68099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="861" mass="97476" checksum="1CD6CFA753AC3924" modified="2005-09-27" version="1">MRIFTVIDAYGLLFRAYYALPNLSTSYGLPIGGVYGFINIFLKYIEKHSTDYLVVVFDTGSKNFRHDIYPEYKSNRPKLPNDLISQFSLLREAVSAFNIASEEVIGYEADDVIATLTKKYSQLQDVEVTVVTSDKDLLQLLNYGIRIFDPIKNRYIEEEDVHNKFGISSNQLLDFLSLTGDASDNIPGVPGIGVKTAAKLLNDFGSLNDLLLRAQEIKTNRCRESIVQYSDQAILSRQLVTLCDTVDICGGIEKYIFQNSEIHELTAFLKKYELQSLINKVNKIFKQNVPSTNRNLNNSSDIVTSDKAIQYSAEGLSVFIENCKSEGIIALHIEIVDNSIGSISLSYRKEVFFYIDKDHVDDALEFIKPIFSLGYILKVIYDVKTLLKIIPNLDIVAFNDIMVMSYVLNPGVHDHSLQNIISYNVEQSITNMNAANLLLLHELLRKSLFVDRLYTVYERVEKPLIRVLDNMEKIGVLVNVDVLKTLSATFSEKINMLESEIYKLSGAEFNIASSKQLGNILFDKMGIKKGKKLSSGNYSTDAEVLSDLAFNDVEIADKILKWRHLTKLKTTYTDVLEKQKNSNSGRVHTFYSMVSTATGRLSSSNPNLQNIPVRSEEGNSIRRAFIAKKGYKLISADYSQIELRIMAHIADVQAFKNAFFLNQDIHSITAKQIFNTEKLDKNLRRRAKSINFGIIYGMSAFGLAKQLSISRSEASTYINNYFRSYPEIQSYMENIKAYAKTHGYTRTIFGRKCFVKDINSDNVTARNFSERAAINAPLQGTSADIIKMSMIHLFDKLKNGSLILQVHDELLFEILEEQVDDAVKIIREVMENIVKLSVPLKVDFNIGDNWADLVPYNSSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT59</accession>
    <name>Q3YT59_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA replication and repair protein RecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">recF</name>
        <name evidence="6" type="ordered locus">Ecaj_0045</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RecF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304174.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0045"/>
    <dbReference type="EnsemblBacteria" id="AAZ68096">
        <property type="protein sequence ID" value="AAZ68096"/>
        <property type="gene ID" value="Ecaj_0045"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0045"/>
    <dbReference type="eggNOG" id="COG1195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040267_2_0_5"/>
    <dbReference type="OMA" id="GESWSYA"/>
    <dbReference type="OrthoDB" id="891841at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00365">
        <property type="entry name" value="RecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001238">
        <property type="entry name" value="DNA-binding_RecF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018078">
        <property type="entry name" value="DNA-binding_RecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042174">
        <property type="entry name" value="RecF_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32182:SF0">
        <property type="entry name" value="PTHR32182:SF0"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00618">
        <property type="entry name" value="RECF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="ATPase_AAA_core" evidence="5">
        <location>
            <begin position="32"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="372" mass="42843" checksum="ACA711A9700ED1E4" modified="2005-09-27" version="1">MPPIEKSYINNLRLVNFRNYSNLELDTSSKSVVLLGKNGAGKTNILEAISLLSKGTGIRGVNTESMQNSTSDSPWSLSYQIHTQNGIYPIVISRNNKQRNIIISNKSQNYITLHKIISITWLIPQLDHIFLKSQSERLRFFDRITHIFDTKYASYIIKYNKAKQERSRLLHNNSTDNFWLSSLESIIAENGINIARTRFNVMQILQSSLSQNSHSNAFFKAVIKIQSQVFDLLDQEDSIELYKEHLKKNRAKDSLSNLVSFGVHNDNFQIFHLEKTLIANNCSTGEQKILLLSLILSSVIAKQNIGEYPILLLDDVMSHLDAFHQEKLIETIINIKCQVWLTDIDLTQQNFAKYREYFKFFHIINNTAILLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT83</accession>
    <name>Q3YT83_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conjugal transfer protein TrbG/VirB9/CagX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrbG/VirB9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0021"/>
    <dbReference type="EnsemblBacteria" id="AAZ68072">
        <property type="protein sequence ID" value="AAZ68072"/>
        <property type="gene ID" value="Ecaj_0021"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0021"/>
    <dbReference type="eggNOG" id="COG3504">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058585_2_0_5"/>
    <dbReference type="OMA" id="MPAIYIE"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06911">
        <property type="entry name" value="VirB9_CagX_TrbG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010258">
        <property type="entry name" value="Conjugal_tfr_TrbG/VirB9/CagX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014148">
        <property type="entry name" value="VirB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033645">
        <property type="entry name" value="VirB9/CagX/TrbG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038161">
        <property type="entry name" value="VirB9/CagX/TrbG_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03524">
        <property type="entry name" value="CagX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02781">
        <property type="entry name" value="VirB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="274" mass="31819" checksum="78CBFF0F0A01B8AC" modified="2005-09-27" version="1">MNFYKIYTTLLIVLIMIMFHGNAHSISIADNPVTIDSRIKTFVYSNNEIYNVVFNYGYHSYIEFSKGETIKMLAIGDTVSWKVKPVDNKLFIIPLEKNGKTNMLIETSKGRSYAFDLICRSAGESNNDKKDVEAEYSDLRDLAYIVRFYYPKTEEDFDLNKINLPDISIFSNSHKIKNHIVIKPNITSDNYLVNTKLEDQKKLPVELFDDGKLTYFKFANNNQIIPQVFIYNNLGEKVPCKMLLLNNYVIIKGVHKNLYLEYKNESVKITNKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT95</accession>
    <name>Q3YT95_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome-c oxidase</fullName>
            <ecNumber evidence="3">7.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cytochrome aa3 subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Cytochrome c oxidase polypeptide III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0009</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome c oxidase subunit 3 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304138.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0009"/>
    <dbReference type="EnsemblBacteria" id="AAZ68060">
        <property type="protein sequence ID" value="AAZ68060"/>
        <property type="gene ID" value="Ecaj_0009"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0009"/>
    <dbReference type="eggNOG" id="COG1845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044071_0_0_5"/>
    <dbReference type="OMA" id="ALLWPDF"/>
    <dbReference type="OrthoDB" id="1502742at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01665">
        <property type="entry name" value="Cyt_c_Oxidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024791">
        <property type="entry name" value="Cyt_c/ubiquinol_Oxase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033945">
        <property type="entry name" value="Cyt_c_oxase_su3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403">
        <property type="entry name" value="PTHR11403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00510">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="SSF81452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="COX3" evidence="11">
        <location>
            <begin position="4"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="274" mass="31294" checksum="65BC9D027B844ACA" modified="2005-09-27" version="1">MKGKLHDHHLVNPSPWPLLFSITVLITALGAVGTIHGTYIGQVMFGLGLPSLSIVLYKWWKDIVTEAIRDNCHTEIVKKGLRLAMAGLILSETMFFFGFFWSFFKAWLFPVYAFDNLIPGKLLNWPPSDIPKLDPWSIPLLNTVILLLSGCTLTWSHYSLINNDNRTTIRMLGATIVLGIIFSTFQAIEYMHTGFSFHETGEKAIYSSNFYMITGFHGLHVIVGTVFLIICLIRAKKNQLSPENHIGFECAAWYWHFVDVIWLLLFVFLYWISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQP8</accession>
    <name>Q3YQP8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EVE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0926</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305030.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0926"/>
    <dbReference type="EnsemblBacteria" id="AAZ68957">
        <property type="protein sequence ID" value="AAZ68957"/>
        <property type="gene ID" value="Ecaj_0926"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0926"/>
    <dbReference type="eggNOG" id="COG2947">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041799_2_1_5"/>
    <dbReference type="OMA" id="LWDGIRN"/>
    <dbReference type="OrthoDB" id="1829674at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002740">
        <property type="entry name" value="EVE_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01878">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EVE" evidence="1">
        <location>
            <begin position="2"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="135" mass="15819" checksum="3D5113D5C9E88017" modified="2005-09-27" version="1">MSYWLLKTEPKDFSWDNMVHDKVTVWDNVLNYQAQNYLKAMKYNDLAFFYHSGKEKVIIGIVAIYKEFYITDINNKFGVVEVKTNKKLNKTVSLKDIKSNSTLKNMIVLKQPRLSVSPITEQEWYCILQLGETAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQQ7</accession>
    <name>Q3YQQ7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305021.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68948">
        <property type="protein sequence ID" value="AAZ68948"/>
        <property type="gene ID" value="Ecaj_0917"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0917"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="CKKIFIT"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230966" evidence="1">
        <location>
            <begin position="26"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="288" mass="31591" checksum="86DCAECB88E9BF5E" modified="2005-09-27" version="1">MNCKRFFIASALISLMSFLPSVSFSESIHEDNINGNFYISAKYMPSASHFGVFSVKEEKNTTTGVFGLKQDWDGATIKDASSSHTIDPSTIFSISNYSFKYENNPFLGFAGAIGYSMGGPRVEFEVSYEIFDVKNQGNSYKNDAHKYCALSRHTGGMPQAGHQNKFVFLKNEGLLDISLMINACYDITIDSMPFSPYICAGIGSDLVSMFETTNPKISYQGKLGVSYSISPEASVFVGGHFHRVIGNEFKDIPAITPAGATEIKGTQFTTVTLNICHFGLELGGRFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQR6</accession>
    <name>Q3YQR6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0908</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305015.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0908"/>
    <dbReference type="EnsemblBacteria" id="AAZ68939">
        <property type="protein sequence ID" value="AAZ68939"/>
        <property type="gene ID" value="Ecaj_0908"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0908"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="KISPYMC"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="292" mass="32862" checksum="2B53AA618BBAD407" modified="2005-09-27" version="1">MNNKSQFLIRFIFLTCMLSLPNISLSKVNNEKHSGLYISGQYKPSVSVFSNFSVKETNFHTKHLIALKQDVDSVEIDTGGNTAGISNPSNFTIPYTAEFQDNHTNCNGSIGYAFAEGPRIEIELSYEKFDVKNPTGYTTVKDAYRYFALAREINISLFQPKQKEGSGIYHVVMKNDGLSILSNIVNICYDFSLNNLPISPYLCGGMGINAIEFFDALHVKFAYQSKAGISYQLLRKINLFIDVYYYQVISNKFKNLKVQHVHELKDNPKVTSAVATLDIAYFGSEAGIRIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQR7</accession>
    <name>Q3YQR7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305014.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0907"/>
    <dbReference type="EnsemblBacteria" id="AAZ68938">
        <property type="protein sequence ID" value="AAZ68938"/>
        <property type="gene ID" value="Ecaj_0907"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0907"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="YTEIQDA"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230968" evidence="1">
        <location>
            <begin position="27"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="299" mass="33085" checksum="14A29F3A1A3CC42D" modified="2005-09-27" version="1">MNSKSKFFTICTSLICLLSSPNTSLSNFIGNSTKHSGLYVSGQYKPSVSIFSKFSVKETNTHTVQLVALKKDVNSISMNISNGATGISKATNFNLPYVAEFQDNAFNFSGAIGYSLFEQLNIEVEGSYEEFDAKNPGGYILNDAFRYFALAREMGQEKNDNKHLSPKEEHDISKTYYTVMRNNGLSILSIMINGCYNLPLNDLSISPYFCTGIGVDAIEFFDALHLKLALQSKIGATYQLSDNISLFTNGYYHQVIGDQFKNLKVQYIGELKENPKITSAVATLNVGYFGGEIGVRLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQS0</accession>
    <name>Q3YQS0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305011.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0904"/>
    <dbReference type="EnsemblBacteria" id="AAZ68935">
        <property type="protein sequence ID" value="AAZ68935"/>
        <property type="gene ID" value="Ecaj_0904"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0904"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="YHRIANN"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230930" evidence="1">
        <location>
            <begin position="28"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="289" mass="32461" checksum="BEABDA87935D424A" modified="2005-09-27" version="1">MNSKKTFSILGSILICLAACLPIQSFSESSNVTYNTKHTGLYISGLYKPSVSHFSDFSIKETYTNTEALFGLKQDISSILRNKETTQYNNNFNVPYTAKFQDDFASFSIAVGYIANNGPRIEIEGSYEEFDVKNPGNYTTIDAHRYIALAREKTSYYLSSPKENKYVIIKNNGISIVSIIINGCYDISLNDSKVSPYICTGFGGDFIEFFSAIRFKFAYQGKIGISYSLSSNIILFTDGYYHKVINSQFKNLNVEHVVNELTTDPKVTSATAFLNIEYFGGEFGLKFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQS3</accession>
    <name>Q3YQS3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0901</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305008.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0901"/>
    <dbReference type="EnsemblBacteria" id="AAZ68932">
        <property type="protein sequence ID" value="AAZ68932"/>
        <property type="gene ID" value="Ecaj_0901"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0901"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="NDAHRYF"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="289" mass="32867" checksum="A1EA73149B7D2F8C" modified="2005-09-27" version="1">MLQRLNFINIILAFLLLLFPFQSFTLYIHDHEITQNVGLYISSQYKPSIPYFKNFLIEENSHKTVELMGLANDVTHVTEYVLKDNTKFNTPYSAKFRNSLINLSGAIGYYSGQGPRLEIEGSYENFDVASCKNCPVKNANRYIALVRDKKPGNIYPSDHSRSNMSYYTFIKNNGISILSVMINGCYDIAFSNVKISPYVCAGIGGDFITLFETMHIKFAYQGKFGISYLVSPSISIFANGHYHKVMDNVFKNLHVKYIYKLQDAPTITSARAKLRIGYFGSEVGVRFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQS6</accession>
    <name>Q3YQS6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305005.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0898"/>
    <dbReference type="EnsemblBacteria" id="AAZ68929">
        <property type="protein sequence ID" value="AAZ68929"/>
        <property type="gene ID" value="Ecaj_0898"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0898"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="NIMDPPI"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230957" evidence="1">
        <location>
            <begin position="23"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="316" mass="35242" checksum="1E09285FF5F6869B" modified="2005-09-27" version="1">MNYAKVFILVCIIFLFPSLSFATNNNYFVHEIGKSIGHFYIGVQYKPGTPHFNRFSIADDSTFNLLAISHTKDYLFSYSTEVRGLFSLPQEQQNLLHYATGGSTTLNTLKDSNKFIPGYNPTYTDNLLGVGGIVGYSINNLRIELEAFYEKFNIKAPTGYNYDTEYFAIATVVYKGKTKPVHYHCMKNTGIILSSFLVNTCYDFTLKIAKKIAPYLCLGVGGDFIDFLGQTRLKASYQAKAGLSYAISPNLTFFVDGSFHGYMNNQFPGLLVDYPTDISVSMPSGDNATAYSEFTTMLAKLNMIFLAGSIGIRFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQS9</accession>
    <name>Q3YQS9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305002.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68926">
        <property type="protein sequence ID" value="AAZ68926"/>
        <property type="gene ID" value="Ecaj_0895"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0895"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="NYRYFAL"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="296" mass="32761" checksum="B4632044F5C20D74" modified="2005-09-27" version="1">MKLLYHLDNIMIKFSAIGIVFSFIALFAPNAFPSPVPIDFSNESEMAGFYASAQYNIGFPRFSPISAKYKTDEKSEKELTLFSLKEETETIDLKKAGDFKKGYSPVYNRNYTGFSGAIGYSGGGLRVELEGSFTRFDVDKQKYKNPDGHRYFALSKDSEIQNSSSGSSSNNKDYVVMKNEGFNAISLMFNACYDMIIGNSSLVPNACIGIGQGIIRFLGGTNIHTLFKAKLGLGFLISPKTILFANGYYVKAKDNAFTNLSVQYPVEISAAPKHIDPIVYFNADNYGCEVGLRFIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQU4</accession>
    <name>LGT_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase</fullName>
            <ecNumber evidence="1">2.5.1.145</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lgt</name>
        <name type="ordered locus">Ecaj_0880</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the diacylglyceryl group from phosphatidylglycerol to the sulfhydryl group of the N-terminal cysteine of a prolipoprotein, the first step in the formation of mature lipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-cysteinyl-[prolipoprotein] = H(+) + S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[prolipoprotein] + sn-glycerol 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:56712"/>
            <dbReference type="Rhea" id="RHEA-COMP:14679"/>
            <dbReference type="Rhea" id="RHEA-COMP:14680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="ChEBI" id="CHEBI:140658"/>
            <dbReference type="EC" id="2.5.1.145"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; lipoprotein biosynthesis (diacylglyceryl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Lgt family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.145" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304987.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YQU4"/>
    <dbReference type="SMR" id="Q3YQU4"/>
    <dbReference type="STRING" id="269484.Ecaj_0880"/>
    <dbReference type="PRIDE" id="Q3YQU4"/>
    <dbReference type="EnsemblBacteria" id="AAZ68911">
        <property type="protein sequence ID" value="AAZ68911"/>
        <property type="gene ID" value="Ecaj_0880"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0880"/>
    <dbReference type="eggNOG" id="COG0682">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013386_1_0_5"/>
    <dbReference type="OMA" id="TDVPWAI"/>
    <dbReference type="OrthoDB" id="435911at2"/>
    <dbReference type="UniPathway" id="UPA00664"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008961">
        <property type="term" value="F:phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01147">
        <property type="entry name" value="Lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001640">
        <property type="entry name" value="Lgt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30589">
        <property type="entry name" value="PTHR30589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01790">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00544">
        <property type="entry name" value="lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01311">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_1000053427" description="Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol)</name>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01147"/>
        </source>
    </evidence>
    <sequence length="261" mass="30279" checksum="4BED2A03E2F67DE0" modified="2005-09-27" version="1">MIIDQVAFRIGPLQIRWYSLSYIFGMIFAYWYIKKIDKYQVFNKESYESVISWWVISVILGGRIGYILFYNLDFYIHFPIEMLKLWNGGMSFHGALVGVMIGMYIFCRKNKIDVLAAFDLGACAVPVGIFFGRIANFINGELYGKVTDIKIGMIFPASGDLLYRHPSQLYEAFGEGFLLFIITNSLFFFTKIKTSKGMLSSVFCIWYGVIRFFIEFVREPDVQIGYIIFDQITMGQLLSIFMIIMGFYFIKLAKVQDKFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQW2</accession>
    <name>Q3YQW2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc metalloprotease</fullName>
            <ecNumber evidence="9">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0862</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304970.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0862"/>
    <dbReference type="EnsemblBacteria" id="AAZ68893">
        <property type="protein sequence ID" value="AAZ68893"/>
        <property type="gene ID" value="Ecaj_0862"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0862"/>
    <dbReference type="eggNOG" id="COG0750">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025778_1_0_5"/>
    <dbReference type="OMA" id="QYMVGFG"/>
    <dbReference type="OrthoDB" id="1395197at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004387">
        <property type="entry name" value="Pept_M50_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42837">
        <property type="entry name" value="PTHR42837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00054">
        <property type="entry name" value="TIGR00054"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M50" evidence="10">
        <location>
            <begin position="22"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ_6" evidence="11">
        <location>
            <begin position="151"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="380" mass="42695" checksum="31653443C9B3DB60" modified="2005-09-27" version="1">MASIIDNIYHILNSGSFYLLSFLTIMSVIVFVHEYGHYIVAKLCNVKIEVFSIGFGPELFGINDKSGTRWKFSIIPIGGYVKMLGDEDPSSSQGGSSHLSEGEKSRAFCEKPLYQKFLIVFAGPLANLIFAIIVLMMFFTTKGIMKHNSVIGGVLQDSVAEHAGLASGDIILKINDHNVKWFEEIKYYIEKYAKDTQELIIEYSRNGHIHTVTIKPSIKEEKGSFGQIKKRAFLGITMSNVLSNYELQRLSVTSAFVQSISYTYLLSKSIFQVLGQMLTGKRSISELGGPIRIAQYSGESVKHNEVLLCMAMISINLGVMNLLPIPMLDGGHIFQYFVQAILRRKQLNPKYQRYVSTIGLMLLLSLMIFVTFNDIKSMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQW5</accession>
    <name>Q3YQW5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine-type D-Ala-D-Ala carboxypeptidase</fullName>
            <ecNumber evidence="4">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ecaj_0859</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the peptidase S11 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0859"/>
    <dbReference type="EnsemblBacteria" id="AAZ68890">
        <property type="protein sequence ID" value="AAZ68890"/>
        <property type="gene ID" value="Ecaj_0859"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0859"/>
    <dbReference type="eggNOG" id="COG1686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027070_8_1_5"/>
    <dbReference type="OMA" id="QNTHFQT"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015956">
        <property type="entry name" value="Peniciliin-bd_prot_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012907">
        <property type="entry name" value="Peptidase_S11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037167">
        <property type="entry name" value="Peptidase_S11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07943">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00936">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69189">
        <property type="entry name" value="SSF69189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBP5_C" evidence="13">
        <location>
            <begin position="272"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAZ68890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="383" mass="43604" checksum="B456C83B8AE03173" modified="2005-09-27" version="1">MMFIMSRYVVLLLSCFFGIQVCINNAIALPLQTKASQVGIFDFLSETMLLEHNIDEQFVSSSMSNLMTLYVTFFYIKAGFVRMEDKFKTSKEAWQKGGNSIFLRAGQLVAVRDLINGIITTSANDACITLAEGISGSQEEFVKEMNRIAQKLNLTKSHFTNVIGVQDQNQVMSARDLITLMVNLFKDFPEYYHLFSKKDFKYNNIYQESINMLLPDNRVDCMIAAHTDTGYGSIVAAKHEGRKIFMFINGLQTKEERLSEIKQLLDYSFNNFSSKTIFNKDNKVKEIAVRGGDSKYVGAAFNKDVTISYPKGSYDTVKTFFSHDNTISAPIKKGQEIGKFHIQIPGFTEQIIPIYATRTVNKISFFEKIWYIFFPKTNEATAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQY5</accession>
    <name>Q3YQY5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CagE, TrbE, VirB family component of typeIV transporter system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0839</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrbE/VirB4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304947.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0839"/>
    <dbReference type="EnsemblBacteria" id="AAZ68870">
        <property type="protein sequence ID" value="AAZ68870"/>
        <property type="gene ID" value="Ecaj_0839"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0839"/>
    <dbReference type="eggNOG" id="COG3451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008341_3_0_5"/>
    <dbReference type="OMA" id="YRPEVYY"/>
    <dbReference type="OrthoDB" id="182908at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018145">
        <property type="entry name" value="CagE_TrbE_VirB_cntrl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03135">
        <property type="entry name" value="CagE_TrbE_VirB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CagE_TrbE_VirB" evidence="2">
        <location>
            <begin position="232"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="791" mass="90498" checksum="8A8DDA276172086D" modified="2005-09-27" version="1">MSFIKEMIGRSSDMHGFSVNGRDGTYNKGDFVPSACHYDENTILNKNGELVQVIKVEDYVLTHHINEKDLRAAVRNSIVNSVKVPEVSFWIYTVRKQHNFDLLQCKTNDVSDVLSSAHVDNISKRVTYINELYIAIVTNHLPESMKGVFGALSFSYIKNKHEDFLKNKVIRLNKITASILESLKKFEAKKLGLVVVDEEKVRSELIEFFYYLTVMRHKECLLDVADISSICSNHDINMGFNTFKVSCDDVQKFGAILAIKDYQESPLDAVDECLQQDYEFIIVEIIKFVKSKNALKLFQKQATFLEFSNDLQLKKLSNIDDFVSVDANSYLNFCERKINFVIMSNTLSQLRGNINKTVSSLAALGIVCVRCDLTMEDDFWSHLPGNFAYILNFKCTLLKYACAFSLLHYFPSGALQGNKWGQAVTMFFSNKGRPYFFNFHVMDKGNTLMVGSPQSSVTMLLNFLLSESRKLNTRIISLDYTGKSIIFIKAIGGKYYRADHRRDYQEMSFNFFQLEDNDLSRKIVTGVLQRMLNVKNITEEMKSSINQVVDHIFTLPIESRTISNIADHISGLGKNVNQWVNNGEFAHLLKEDSNIDWKAKVLGLNVGIVFSKPQCASVIIYYFLNMLINYLDGSPTILVIDEAWILDYIFVNDKEFDEWIDTMNKLNVVIVFTGENIPAIVSSNIIYKFNQYVETQIFMPNSVSTSKMYMKAFNLSKSECNTMFQMPSQGGYFFIKQDSASVVLSFNLPNIPETNILVANKNTIRYMYESINSHGDDVKEWLPTFYKKCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQZ6</accession>
    <name>Q3YQZ6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aconitate hydratase</fullName>
            <shortName evidence="10">Aconitase</shortName>
            <ecNumber evidence="10">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ecaj_0828</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. Could catalyze the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate. The apo form of AcnA functions as a RNA-binding regulatory protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <text evidence="10">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0828"/>
    <dbReference type="EnsemblBacteria" id="AAZ68859">
        <property type="protein sequence ID" value="AAZ68859"/>
        <property type="gene ID" value="Ecaj_0828"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0828"/>
    <dbReference type="eggNOG" id="COG1048">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013476_2_1_5"/>
    <dbReference type="OMA" id="NGGIMQY"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00718"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047780">
        <property type="term" value="F:citrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="PTHR11670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 10 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase" evidence="11">
        <location>
            <begin position="72"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase_C" evidence="12">
        <location>
            <begin position="680"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="883" mass="97223" checksum="4B8F79529E4E12D1" modified="2005-09-27" version="1">MELLNSLDSKKKLITGKSYYEYFSLKAACSKLGVDIAKLPYSLKVLFENLLRNENGHSVRLEDIKKLAQCVNEHVKYEINFSPARVLMQDFTGVPAVVDLAAMRDYVRENGENPSIINPKVPVDLIIDHSIQVDFYGEEDAFNKNVSVEIQRNLERYKFLKWGQHAFKNFRVVPPGAGICHQVNLEHIAKVVWSEGDLVYPDTLVGTDSHTTMINGLSVLGWGVGGIEAEAVMLGQPITMLIPQVIGFKLTGKLSEGVTATDMVLTITSILRSKGVVGKFVEFYGDGLSGLSIADRSTVANMSPEYGATCGFFPIDHKTLEYLEITGRDISLIDLVESYAKEQCLWHTKEEPRYDDRIELDLASVQPVIAGPKRPQDKIFLSDVKATVQNLLQSSKSTVIDPESQSLRNGDVVIAAITSCTNTSNPSVMIAAGLVARKANMLNLKAKPWVKTSLAPGSQVVTEYLVKSGLQKDLDALGFHLVGYGCTTCIGNSGPLSSKIEEDIKRNDLIVASVLSGNRNFEGRIHPCIRANFLASPPLVVAYALAGTVDINLNADPIAVDDDGKHVYLHDIWPSNDEINEVISKAIDKQMFVDKYKDVFSGGKYWEDLKCANGDIYLWDTSSTYIQSPTYFKNLSIAVDDASNNINISNARILALLGDSVTTDHISPAGNIAANSPAGMFLMQNNVVVQDFNSYGSRRGNHHVMTRGTFANIRVRNEMVNCEGGFTKYVPTGEKMSIFDASQLYQQNNVPLVVIAGKEYGSGSSRDWAAKGTFFLNIKAIIAESFERIHRSNLIGMGVLPLTFKEGDTRKTLQLTGDEGISIVGEIGINKEVECIIFRKDGVSQNIKLICNLNTAIEVDYFKSGGVLQYVLVRMMRSNLSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQZ9</accession>
    <name>Q3YQZ9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0825</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the MIP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304933.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0825"/>
    <dbReference type="EnsemblBacteria" id="AAZ68856">
        <property type="protein sequence ID" value="AAZ68856"/>
        <property type="gene ID" value="Ecaj_0825"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0825"/>
    <dbReference type="eggNOG" id="COG0489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_0_5"/>
    <dbReference type="OMA" id="QHITFKD"/>
    <dbReference type="OrthoDB" id="1413173at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="PTHR42961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="7">
        <location>
            <begin position="4"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="111"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="351" mass="38537" checksum="E96D733AF059747C" modified="2005-09-27" version="1">MITKQDVLNALLAVVDCNSNRSIVELGLVTSVLIKDNVVYCVLNLLNEHQIAQKNVIEKKCNDAINIIPHVKGIKIVFTAVHDTVNKVNRRAPTEKISITGVKSLILISSGKGGVGKSTLAMNIAFSLLRKGYRTALADLDIYGPSIPHMLGVKDFANPEIDNDNKMIPITRYGLRSMSIGYLIAKDNAAIWRGPMVTKAIYTLMTNTAWEEVDYLIIDTPPGTGDVHLSLVEKFDITGVVVVSTPQDLAIIDAVKICDMMKKMNVHIVGIVENMSYFIDTNSGNKTYVFGKQGVHKVANELNIDFLGEVPLYPQICSVAELGTPLSLDHEICEIYDNITNNMLCSLKSTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR00</accession>
    <name>Q3YR00_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304932.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0824"/>
    <dbReference type="EnsemblBacteria" id="AAZ68855">
        <property type="protein sequence ID" value="AAZ68855"/>
        <property type="gene ID" value="Ecaj_0824"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0824"/>
    <dbReference type="eggNOG" id="COG3827">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1381605_0_0_5"/>
    <dbReference type="OMA" id="KPELSTW"/>
    <dbReference type="OrthoDB" id="1294633at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019632">
        <property type="entry name" value="DUF2497"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10691">
        <property type="entry name" value="DUF2497"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="242" mass="27661" checksum="F2C85BD2CC7CD02F" modified="2005-09-27" version="1">MSNTNDFQSIKDVIANIRKVMSSNDNGDSTTEQHDTNTQPDDTEHEVLNLENPENHVELHNIQHAHNTLNEINQTFQAISDLPITTYQNKTQPDIQISVKKEKIYPDQLSNVNQFISIEQSTQRLSSQERRLTTTHTLSEEISKNTSEIKGVHQKNTFISENLVSPESIVASSEEIKKLITQVHNYTKPTNVSSDKSPTIEELVINMLKPELSTWLNNNLQKLVKEIVEKEIKHIIKKSNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR07</accession>
    <name>Q3YR07_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Response regulator receiver: C terminal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0817</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304925.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0817"/>
    <dbReference type="EnsemblBacteria" id="AAZ68848">
        <property type="protein sequence ID" value="AAZ68848"/>
        <property type="gene ID" value="Ecaj_0817"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0817"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_5"/>
    <dbReference type="OMA" id="QLWGYPP"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="127"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="127"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="266" mass="30109" checksum="24C27E427A38A202" modified="2005-09-27" version="1">MRILLIEDDIACAKAVEASLSSEGHFCETMTSAQDCYGSIIPKNDDYDVVILDIHFPGKIDGYDILVKLRESGIKVPVLILSCISSVSHKVKGLYYGADDYVTKPFHKSELLARIKAIVRRTRGHPESIVRVGNIAINLDHKCVECNGEIVHLTKKEYGMIELLSSRLGTVLTKEMFLSHLYRELDEPSDDKIIDVFMCKLRKKLKVANNGKNYIETVWGRGYVLKECVDEDENDDREGYRWDHDGGSNIDSVYGAQEDKKYVEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR25</accession>
    <name>Q3YR25_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0799</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304907.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68830">
        <property type="protein sequence ID" value="AAZ68830"/>
        <property type="gene ID" value="Ecaj_0799"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0799"/>
    <dbReference type="HOGENOM" id="CLU_577136_0_0_5"/>
    <dbReference type="OMA" id="YPWYSII"/>
    <dbReference type="OrthoDB" id="979701at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="474" mass="55907" checksum="0885E9D377D46AFD" modified="2005-09-27" version="1">MYQRKYSQTINEILEIISSNSLHSTDSLNLLLVIVPQIYNVPSTDNENYRYYHIIQHYTNEAYNNIQNKEQAKLLLKLWFKALIHKVSYGITIFPEDQKIIDKTLDKLFNKFRKINQYPTQQIKDKLYNKFRRIISKSNINPELKEFFQSYATDTSNLTKHKMEVINEIIASVNTQDVTLLGTCHGITKGYSKNYLNIPISQKIKYLYQDIMKTEPIDLKHALKLFPKKHPVFSIQYQLTKLHVTLKDYQNRKEKFSTAKYPWYSIINNIKKVIYTHIIQPKLTKKAHILTQITSAFKESIAYGDTVFFFKQLKAIHKNSNISWNPFYKDINTLIKSLNNMEPPVQNIMTSKEFITNIKFYPKHEHTTHIVRNTKKQHFLNNLHTGSTHTKTEHNKFHKRNIIRKAKKAISYTNKIAQENIKSIKNRITKTIHKKRKKTHKIHTTPTTSFAFIDLISSDELTNTNTNILSTIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR31</accession>
    <name>Q3YR31_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304901.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0793"/>
    <dbReference type="EnsemblBacteria" id="AAZ68824">
        <property type="protein sequence ID" value="AAZ68824"/>
        <property type="gene ID" value="Ecaj_0793"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0793"/>
    <dbReference type="eggNOG" id="COG0742">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075826_0_2_5"/>
    <dbReference type="OMA" id="FNWLMPY"/>
    <dbReference type="OrthoDB" id="1827334at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004398">
        <property type="entry name" value="RNA_MeTrfase_RsmD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004553">
        <property type="entry name" value="CHP00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00095">
        <property type="entry name" value="TIGR00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="182" mass="20372" checksum="2C17FDABF40536B3" modified="2005-09-27" version="1">MLRITSGKYRGRRIFSDKLLSARPAMSIIRESIFNIILSRMSIQGCKVLDLFCGSGSLSFEALSRGAESALLIDINHYNLSLVKRTSEHLGVVNNVTLMCCDVEKLPIATDQYDIIFVDPPYTNPALVDIALSVLMRLNWIRSSSVVVVRVRKKVAFICPAGYKVMVERIYGISKVIILQCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR36</accession>
    <name>Q3YR36_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0788</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0788"/>
    <dbReference type="EnsemblBacteria" id="AAZ68819">
        <property type="protein sequence ID" value="AAZ68819"/>
        <property type="gene ID" value="Ecaj_0788"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0788"/>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_2_5"/>
    <dbReference type="OMA" id="KIKICKF"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="5">
        <location>
            <begin position="36"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="110" mass="12367" checksum="81330F10864DA8A8" modified="2005-09-27" version="1">MVSVMIEQISDSDFHSKVISCNEDILILVDFWAPWCGPCKTLEPQLEKLAQQYAEQVKIYKLSIEDNQDVAIQYGVSAVPTTLMFKNGKKLSQVIGADIAKIINELNNHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR42</accession>
    <name>Q3YR42_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">50S ribosomal protein L31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0782</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL31 family. Type A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0782"/>
    <dbReference type="EnsemblBacteria" id="AAZ68813">
        <property type="protein sequence ID" value="AAZ68813"/>
        <property type="gene ID" value="Ecaj_0782"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0782"/>
    <dbReference type="eggNOG" id="COG0254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114306_3_0_5"/>
    <dbReference type="OMA" id="PETHPAW"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002150">
        <property type="entry name" value="Ribosomal_L31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042105">
        <property type="entry name" value="Ribosomal_L31_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01197">
        <property type="entry name" value="Ribosomal_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00105">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 8" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="102" mass="12071" checksum="9D9FBF79F23DB67B" modified="2005-09-27" version="1">MTNDTYKCYKHRLQINLYYKTFEKVYRMKKGIHPETNDILTIFTNGEERKMKSTLGKKNELIKIHLESDCFNHPAWNPNLRTVSKKNSKAAKFISKFGDLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR57</accession>
    <name>Q3YR57_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0767</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68798">
        <property type="protein sequence ID" value="AAZ68798"/>
        <property type="gene ID" value="Ecaj_0767"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0767"/>
    <dbReference type="HOGENOM" id="CLU_2422264_0_0_5"/>
    <dbReference type="OrthoDB" id="2232347at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="91" mass="9900" checksum="21640716006FEC7D" modified="2005-09-27" version="1">MGNSGISIIVCVVFIFLIMMVLVSMFQRNASIFDCVSRQQCKASNADHDKGNDTSNQSEEASVNTDLVSGSNGTCEDIDLQSVQSRKCCRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR58</accession>
    <name>Q3YR58_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304874.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68797">
        <property type="protein sequence ID" value="AAZ68797"/>
        <property type="gene ID" value="Ecaj_0766"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0766"/>
    <dbReference type="HOGENOM" id="CLU_2860610_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="64" mass="7528" checksum="4903F7B04D5F84BD" modified="2005-09-27" version="1">MGNNDDHKIKCMECLFSLVDVFMCLIMSFAIFGILRMAHDRTEQRSENQNVEPVALRNRLRQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR61</accession>
    <name>Q3YR61_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0762</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68794">
        <property type="protein sequence ID" value="AAZ68794"/>
        <property type="gene ID" value="Ecaj_0762"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0762"/>
    <dbReference type="HOGENOM" id="CLU_784665_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="353" mass="37327" checksum="1A410AFF915EDAA5" modified="2005-09-27" version="1">MPTQTTETFSSTVSTAVESTTSSASTVTGHNTLNTITDTASTVTSAITDIASSTLTSITNYTSNTINATSEVITTAVSSVAETTASIATTVMNATSSAVSTAANATSSVISTAAENVTTALTTSTEILSNITSTAVNATSSAISTAAENVTTALTTAIETATNIANTAINTTSSIANTIPRTSRSTTTYTAQSSDHHLKIVAATLFAIVLILIAITLIVNLNRKCNKDRTRRNSDEEIQALLQLVSTVDRSAGSETQNVGLDSFTMENQEELLYEPRRRSPYQEFIAFEDGSILPVSRRCTNREPITAVIMVSEDSDEEDQEHESHDTSDDGTIPGSVTEPMIQYHCNKHAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR70</accession>
    <name>Q3YR70_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68785">
        <property type="protein sequence ID" value="AAZ68785"/>
        <property type="gene ID" value="Ecaj_0753"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0753"/>
    <dbReference type="HOGENOM" id="CLU_1025775_0_0_5"/>
    <dbReference type="OrthoDB" id="2115437at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="271" mass="31260" checksum="2947141B9A757670" modified="2005-09-27" version="1">MTFYQQQVAVMLIFIILFFLTLMCMIYVCYRMSCFNHKLERACDFVREEVEEDIDEKLVGINSTGDRCLCQITSAIDYIMTQFEILDNCVGKEEDVESAKEDFFLGKMIARIIGVYQSESKLSEYEKSILKQLKLKYAESSPTDIRAESLLYIKNFVNKVCTNVRSSFPRHIDLSPKLLILGRMKVAFEILENCMKCVRQDITLERFFSVMGKSTEEELIETVISKDPELTLRLSQSVRKNVPKNNESCCTGSDVSDVIRLILEVGYLVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR76</accession>
    <name>Q3YR76_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0747</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304856.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68779">
        <property type="protein sequence ID" value="AAZ68779"/>
        <property type="gene ID" value="Ecaj_0747"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0747"/>
    <dbReference type="HOGENOM" id="CLU_1832009_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="140" mass="16153" checksum="17E75726664ED01E" modified="2005-09-27" version="1">MENVPKLLLISVIVILLLLIAAVCYSVYNRNKQTVLSLQEEVKDLRNRLEMLSGNFERFYDSFFSSSVGRQIRYSNHLVESIKDEVSDMEENIITNSCMIKQLKDNLINASETMLSNIQVLDCKIESMMLDRERSIKPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR84</accession>
    <name>Q3YR84_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0739</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304848.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68771">
        <property type="protein sequence ID" value="AAZ68771"/>
        <property type="gene ID" value="Ecaj_0739"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0739"/>
    <dbReference type="HOGENOM" id="CLU_1254318_0_0_5"/>
    <dbReference type="OrthoDB" id="2181829at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="220" mass="24608" checksum="157F7FA826F005BB" modified="2005-09-27" version="1">MDNRLITVAIALLAAFILIFACLLIVAIYKCCVSTKKVAEDEKPLLEDGGPSRDELLQTVEKLESDFQRALSGEFQQKLENRSLLRDLGKERECVAQLQKECDLLLEKNKALAAQSEKLELMLSREHEIKMIKDKRGTTNTRPRALAWGFDLMKKCSNAVAGIRSSTHVSESDKELITQQVLLLKESVQYFLNSISYSELPQTVMSDVEQSGVSGGKNVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR90</accession>
    <name>Q3YR90_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68765">
        <property type="protein sequence ID" value="AAZ68765"/>
        <property type="gene ID" value="Ecaj_0733"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0733"/>
    <dbReference type="HOGENOM" id="CLU_1658089_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="159" mass="18226" checksum="BD44A4DD67F0CADF" modified="2005-09-27" version="1">MLLLVIFLIYQRCFKPLRDKVDMLEYENKLLEEKNAELESEISDVSKARDAVLSKYSDVLENIVGTLDPEVKVSKTGDFFQDATANVTVIARLMQQSVSDFVKYIRGNEEKQKKQYKEQGVQVGSVSLVSNKACYLVYYCFVVVMSLMQNSILEFIRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR96</accession>
    <name>Q3YR96_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0727</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304836.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68759">
        <property type="protein sequence ID" value="AAZ68759"/>
        <property type="gene ID" value="Ecaj_0727"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0727"/>
    <dbReference type="HOGENOM" id="CLU_2342318_0_0_5"/>
    <dbReference type="OrthoDB" id="2197187at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="97" mass="10665" checksum="0F7C39FFDBA56EA7" modified="2005-09-27" version="1">MQMESDVVQTSKFRLKLLISELVYKLGKNCTSVIATCCDLSLSEKLALSSSMLTLERTMLNLVDSAVGEASLATVPNTQLFSTFLQCNNVNCPEKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRA3</accession>
    <name>Q3YRA3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68752">
        <property type="protein sequence ID" value="AAZ68752"/>
        <property type="gene ID" value="Ecaj_0720"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0720"/>
    <dbReference type="HOGENOM" id="CLU_1249005_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="221" mass="24681" checksum="815A1B8615DEF7AE" modified="2005-09-27" version="1">MNMGTKEIIGIVIAFIVICALISCLVYKLVKQSQEEKKILKLLNSEKPLASFPEMLQLAKLLKSITTCSANFESLSYCCCNMSCVICYIHKSCVVDNKAKKTIRRLEREVIFCRNALVLVETRMLECPLSDKKLCELLVAVSEKLECTLDEMMNIFVNTCAAPGQKEIFAANCEGLLNDRKTLQKVKKYCESIVNGSVTQSNSKLDVNGLRCVGNSSERSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-04-07" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRB0</accession>
    <name>Q3YRB0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="8">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purC</name>
        <name evidence="10" type="ordered locus">Ecaj_0713</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0713"/>
    <dbReference type="EnsemblBacteria" id="AAZ68745">
        <property type="protein sequence ID" value="AAZ68745"/>
        <property type="gene ID" value="Ecaj_0713"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0713"/>
    <dbReference type="eggNOG" id="COG0152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061495_2_0_5"/>
    <dbReference type="OMA" id="EFCYKND"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01415">
        <property type="entry name" value="SAICAR_synt_PurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033934">
        <property type="entry name" value="SAICAR_synt_PurC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR_synt" evidence="9">
        <location>
            <begin position="15"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="258" mass="29721" checksum="C0ACA831E756FF3B" modified="2005-09-27" version="1">MVNGSHKYIMKNREKIYEGKAKIIFATSNPLEVIQHFKDEVTAFNNKKADVINEKGIVNNYISSFLMKELTNKGIKTHFISLLNQREQLVKKVSIIPLEIVVRNLSAGNFSKRFQIADGTSFKSPIIEFYYKSDKLSDPMVSDGHILSFQWLTNQELEKIKMLSLKINEILSEIFLNVGIKLVDFKLEFGKLYNNKASDLLLADEISPDTCRLWNISDNRKLDKDLYRMNLGNVIEAYKEVAQKLNVITTKHIIDNNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRB6</accession>
    <name>DCD_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dCTP deaminase</fullName>
            <ecNumber evidence="1">3.5.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Deoxycytidine triphosphate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dcd</name>
        <name type="ordered locus">Ecaj_0707</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the deamination of dCTP to dUTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dCTP + H(+) + H2O = dUTP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="EC" id="3.5.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dCTP deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304816.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRB6"/>
    <dbReference type="SMR" id="Q3YRB6"/>
    <dbReference type="STRING" id="269484.Ecaj_0707"/>
    <dbReference type="EnsemblBacteria" id="AAZ68739">
        <property type="protein sequence ID" value="AAZ68739"/>
        <property type="gene ID" value="Ecaj_0707"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0707"/>
    <dbReference type="eggNOG" id="COG0717">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087476_4_0_5"/>
    <dbReference type="OMA" id="FENHRYP"/>
    <dbReference type="OrthoDB" id="1598407at2"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00665"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000009719" description="dCTP deaminase">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="107"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <sequence length="187" mass="21098" checksum="E719F09D0A5071C0" modified="2005-09-27" version="1">MSVMPDHWIKDKAIQEKMIDPFIDYKESSGILSYGLSSYGYDARIDNKFKIFTNINPVTVDPKNFPLGSFIDKEEDICIIPPNSFVLAKTVEYFKIPKDILVMCVGKSTYARCGIVVNVTPLEPGWEGYVTLEFSNTTPLPAKIYAFEGACQFIFLKGDSQCSLSYNEMKGKYMKQLDVTLPIVNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRC5</accession>
    <name>Q3YRC5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">DNA repair protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">radA</name>
        <name evidence="9" type="ordered locus">Ecaj_0698</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Plays a role in repairing double-strand DNA breaks, probably involving stabilizing or processing branched DNA or blocked replication forks.</text>
    </comment>
    <comment type="domain">
        <text evidence="6">The middle region has homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the RecA family. RadA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304807.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0698"/>
    <dbReference type="EnsemblBacteria" id="AAZ68730">
        <property type="protein sequence ID" value="AAZ68730"/>
        <property type="gene ID" value="Ecaj_0698"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0698"/>
    <dbReference type="eggNOG" id="COG1066">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018264_0_0_5"/>
    <dbReference type="OMA" id="FELMRLA"/>
    <dbReference type="OrthoDB" id="505485at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01498">
        <property type="entry name" value="RadA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004504">
        <property type="entry name" value="DNA_repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041166">
        <property type="entry name" value="Rubredoxin_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18073">
        <property type="entry name" value="Rubredoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00416">
        <property type="entry name" value="sms"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RECA_2" evidence="8">
        <location>
            <begin position="62"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Lon-protease-like" evidence="6">
        <location>
            <begin position="349"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RadA KNRFG motif" evidence="6">
        <location>
            <begin position="249"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="91"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="450" mass="49309" checksum="3A6810EA0CBEA44C" modified="2005-09-27" version="1">MSTKVLYICQVCGNQTHRWTGKCNSCGSWDSITGETATEHLINTQHNSEIKLSSLSDKNVEISSRFITKISELDRVFGGGIVLGSSTLVGGEPGVGKSTLLLQLTSILDKNFSKCLYVSGEESIDQIKIRADRLQITQSNIQLLSTSSLSDIILSLKKNKDFKCVVIDSIQTIYDSRISSSPGTVVQIRMCTHELITFTKQHNIILFILGQITKDGQIAGPKTLEHMVDTVLYFEGENNSQLRILRTVKNRFGPTNEIGVFEMSDKGLIPVKNPSYLFLAKSQNNNIVGSAVFAGIEGSRPILMEIQSLIANTNMVTPRRAVVGWDVNRLAMIIAVLTAKCNIFLGDKEVYLNIAGGLKICEPAADLAIAASLISSFINVAIPSSIIILGEIALSGEVRNISSIDSRLKEAHKLGFTKAIIPLNNKHVFTDINTVEIGHIKMLKKYLIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRD2</accession>
    <name>Q3YRD2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CyaY protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0691</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the frataxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304800.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0691"/>
    <dbReference type="EnsemblBacteria" id="AAZ68723">
        <property type="protein sequence ID" value="AAZ68723"/>
        <property type="gene ID" value="Ecaj_0691"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0691"/>
    <dbReference type="eggNOG" id="COG1965">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080880_4_1_5"/>
    <dbReference type="OMA" id="IVSKHEP"/>
    <dbReference type="OrthoDB" id="1812841at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002908">
        <property type="entry name" value="Frataxin/CyaY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036524">
        <property type="entry name" value="Frataxin/CyaY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020895">
        <property type="entry name" value="Frataxin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821">
        <property type="entry name" value="PTHR16821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01491">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01219">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55387">
        <property type="entry name" value="SSF55387"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03421">
        <property type="entry name" value="FeS_CyaY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01344">
        <property type="entry name" value="FRATAXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50810">
        <property type="entry name" value="FRATAXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="109" mass="12367" checksum="1680B42F039DF126" modified="2005-09-27" version="1">MSHSLCISDFQALSYNLLNTLTEMIDRVDIDGVLECENCDGVVKISDSNGNLYIINKHEPSMQIWVASPISGSVRFSYDKSLSAWINDKSDELFNFLRVEMKVLFDIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRE4</accession>
    <name>Q3YRE4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304788.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0679"/>
    <dbReference type="ESTHER" id="ehrcj-q3yre4">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68711">
        <property type="protein sequence ID" value="AAZ68711"/>
        <property type="gene ID" value="Ecaj_0679"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0679"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066961_0_0_5"/>
    <dbReference type="OMA" id="TISRWLE"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="28"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="257" mass="28843" checksum="ECC7DEC140178F80" modified="2005-09-27" version="1">MSGYATLTLSYAQDLHIAYKQLVGDKVSIIFFGGFNSNMQGTKATALYDYCKLHNLGLIIFDYLGHGESDGEFTDYNISDWYKNCIEVMNQLTPVDKPQIIIGSSMGAWLMLLAAISNQDKVSHLISLAGAPDFTESLIFQKLNAIQKDELYKNGKITLYANSNKTHSYLITRNLIEDGRKHLLLHQESINITCSVTLIHGMKDDTVPYQVSITLAEKIKSNNINLHLIKSADHNLSDDNSINIILRYVKEAVDQSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRE7</accession>
    <name>Q3YRE7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0676</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304785.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0676"/>
    <dbReference type="EnsemblBacteria" id="AAZ68708">
        <property type="protein sequence ID" value="AAZ68708"/>
        <property type="gene ID" value="Ecaj_0676"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0676"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1228334_0_0_5"/>
    <dbReference type="OMA" id="HAINAKP"/>
    <dbReference type="OrthoDB" id="1575094at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="229" mass="25685" checksum="3D92A02000E80B5D" modified="2005-09-27" version="1">MCILGADKIIPPHIPAVGTGKGYSSDGIAIPRNSKEILKNKITPGTKPLVVFIGGALDDTTRLVLRLYARYYTQNHDHQDIAYATWGSSATIPIIKAWYEAEQKICLVGHSWGGDSEYKIVKKLEMNTIDLMCTIDPVSRAGVGGKLPKPKNLKKWYNVAIDYKKVKFSINNLIAQTGGPWFYCMYADKNFIVDTVEINKHLVSADHAMAEALFFKYFNEYVKNFATQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRF5</accession>
    <name>Q3YRF5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative response regulator NtrX-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ecaj_0668</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304777.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0668"/>
    <dbReference type="EnsemblBacteria" id="AAZ68700">
        <property type="protein sequence ID" value="AAZ68700"/>
        <property type="gene ID" value="Ecaj_0668"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0668"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_0_6_5"/>
    <dbReference type="OMA" id="PTNSRVM"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="10">
        <location>
            <begin position="16"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="9">
        <location>
            <begin position="153"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="8">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="471" mass="53160" checksum="FCFE98C3D30F56D4" modified="2005-09-27" version="1">MAQDFEMSKERLYISEVLVVDDEGDIRNLIKDILSDDNHGTKLAVDGLSAIKMAYEKEPDVVLLDIWLKGSDIDGLSVLEKLKERYPHLPVIVISGHGNIATAVKSLHIGAYDYIEKPFTESRLKLVVKRAIESGRLRRENDELKSAFEDYEIVGSSSVIKNLRSMVNKAAATSSRILITGSPGVGKEVVARLIHKKSKGYDTPFISMYSSMLPANNYLVNIFGSEESSNILSHRVPPHIGIIEQANRGTLFIDEVTDLRYDTQLRLLRLLQEGKIYRENSKVPVSVDVRIIVSSSKDIENEVRAGKFCEDLYYRLNVLPIRVPSLVEYCTDIPELCRYFMNSICKKMGLCTHILSDEALIAMQSYEWPGNLRQLRNVIEWILIMKSPKEVITAKDLPVDIVSNSPINDVLSAKVISVPLRQAREEFERQYLKTQLSRFGGNVSKTAEFVGMERSALHRKLKVLGLCNISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRG9</accession>
    <name>Q3YRG9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fructose-1,6-bisphosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0654</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the FBPase class 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304763.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0654"/>
    <dbReference type="EnsemblBacteria" id="AAZ68686">
        <property type="protein sequence ID" value="AAZ68686"/>
        <property type="gene ID" value="Ecaj_0654"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0654"/>
    <dbReference type="eggNOG" id="COG1494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054938_0_0_5"/>
    <dbReference type="OMA" id="AVFYMDK"/>
    <dbReference type="OrthoDB" id="879338at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01516">
        <property type="entry name" value="FBPase_glpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004464">
        <property type="entry name" value="FBPase_class-2/SBPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30447">
        <property type="entry name" value="PTHR30447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03320">
        <property type="entry name" value="FBPase_glpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004532">
        <property type="entry name" value="GlpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00330">
        <property type="entry name" value="glpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="86"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="163"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR004532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR004532-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR004532-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="309" mass="33167" checksum="362F46814426A9A1" modified="2005-09-27" version="1">MQNLCFKLLSVTEAAALASYKCLGMGDEKGADRVAVDAMRKSLNLLEISGTVVIGEGERDNAPMLYIGEKVGTGGIDVDIALDPLEGTTMCAHYKNGAMSVLAVTEKGGFLNAPDVYMEKIAVGPNIPRGVISLKYDVKTNLYNLAAVKKCKVDELVVTVLYRERHLELIKNIRECGVRIKLIDDGDVSAVVSLMKGMHDLYVGIGGAPEGVLAAAALLSMGGYMEGKLVLDTESLKTRAEKFGIKDPDKIYSIEEMVKSDAIFLATGITDSSLVQGIKYSQGSFMIESVIIKPASIMYINNTIPESVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRH6</accession>
    <name>Q3YRH6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Chaperone protein ClpB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">clpB</name>
        <name evidence="15" type="ordered locus">Ecaj_0645</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homohexamer. The oligomerization is ATP-dependent.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homohexamer; The oligomerization is ATP-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304756.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0645"/>
    <dbReference type="EnsemblBacteria" id="AAZ68679">
        <property type="protein sequence ID" value="AAZ68679"/>
        <property type="gene ID" value="Ecaj_0645"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0645"/>
    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_4_0_5"/>
    <dbReference type="OMA" id="SKMMQGE"/>
    <dbReference type="OrthoDB" id="44062at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017730">
        <property type="entry name" value="Chaperonin_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03346">
        <property type="entry name" value="chaperone_ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Clp R" evidence="14">
        <location>
            <begin position="3"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="414"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAZ68679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="857" mass="95495" checksum="F87C4B5CE3809334" modified="2005-09-27" version="1">MDLNKFTDVSKNLIMQSQTAAVALGHQSLVPEHLLKVMLDEKDELVELLLTSCGCDIDKIYNDVKLSLNKLPVVSGSGSGHIHLSREMAQVLEEAVSLAKRNQDTYVTVERLLQALAIVKDSNVCKILSAHGVTPVKLESLILNMRNGTKADSVNSEQKFNALKKYAKDITEAAMAGKLDPVIGRDEEIRRTMQVLSRRTKNNPVLIGDPGVGKTAIIEGLAQRVVIGDVPVGLRNTKIMALDLGMLVAGTKYRGEFEERLKAVINEIVASNGAVILFIDELHTLVGAGATDGAMDASNLLKPALARGEIHCIGATTLDEYRQHIEKDAALARRFQPVFISEPTVNDTISILRGLKEKYEIHHGIRIMDSAIIAASTLSNRYITDRFLPDKAIDLIDEAASRARIEIDSKPEVIDELDRKIIQLKIEAGVLEKENTESSKQRLVKLNEELDKLNIQAAELNSKWQAEKIKILKMQECAEKLDNARSDLEKAQRSGNLAKAGELMYGIIPELEKELKKCEKPSSNMLKREVTEDDIANIVSRWTGIPIENMMSSEREKLLRMEEEIGKTVIGQESAIKAVSDAVRRSRAGVQDANKPLGSFLFLGPTGVGKTELVKTLAEFLFCDRSALLRFDMSEFMEKHAVSRLIGAPPGYVGYDQGGMLTEAVRRRPYQVILFDEIEKAHGDIFNILLQVLDEGRLTDNHGKLVDFRNTILVLTSNLGQEILINTEEDVDEDSIKQSMTSVLQHHFRPEFLNRLDEIIIFHKLTKAHIEKIIDVQFSILQKIVAQKKLEISLSLRAKSWLIDNGYDSLYGARPLKRLIQQRIQNSLAKLILANQVNEGNKLEVDVSGDDLVINKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRJ0</accession>
    <name>Q3YRJ0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Type I secretion system ATPase, PrtD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ecaj_0631</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of an ABC transporter complex. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304742.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0631"/>
    <dbReference type="EnsemblBacteria" id="AAZ68665">
        <property type="protein sequence ID" value="AAZ68665"/>
        <property type="gene ID" value="Ecaj_0631"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0631"/>
    <dbReference type="eggNOG" id="COG4618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_95_6_5"/>
    <dbReference type="OMA" id="WFGWMAL"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030256">
        <property type="term" value="C:type I protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030253">
        <property type="term" value="P:protein secretion by the type I secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010128">
        <property type="entry name" value="ATPase_T1SS_PrtD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24222:SF0">
        <property type="entry name" value="PTHR24222:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01842">
        <property type="entry name" value="type_I_sec_PrtD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="31"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="339"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="568"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="373"/>
            <end position="380"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="592" mass="64641" checksum="54D360B10AA73D64" modified="2005-09-27" version="1">MEGVNFNFQAMQELKKSVLYNSLEKCKSVFWFIFWFSSAINLLMLFLPLYTSQVLDRVLSSGSVSTLIMLSVITVGAFACSSILEICRSLVMARVGDWIDKVVTPDLIMKSISLTSIKSSTSSGEVLRDLSVVKSFITGYGIFSLFDTPWAILYLVAIFMIHSVTGYIAVIGIVLLTSMAIWNELATKKILQEASEEGIRNINAIDVATRNAEVVEAMGMVNYIVDDWAEKNDRNRAMQIKAQNRSNLISGITKFIRSVLQISVIGVGAFLAVLGHKTAGGIIASSILMGRALAPFEALINTWKMLISARISYKRLQILLLASPKREQTMSLPAPAGKVVFDRVFFTPYGGNKPTLKGVSFAVDVGDIVGVIGHSASGKSTIAKLLVGVWKPISGVVRLDGADVYTWNREDFGRYIGYLPQDVELFNASVKVNIARMVPDPDPDKVVRAAQIAGVHELILSLPNGYDTLIGFNGVVLSGGQKQMLGLARAFYGDVKLLVLDEPNANLDGKSEASLVNALLYARKNGITTFIMTHKIQLLNAVDKVIIMNDGMVNGMGSRDEILSKIVPSYTPPNSQPQQQPKPLVQSEDVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRJ9</accession>
    <name>Q3YRJ9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304733.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0622"/>
    <dbReference type="EnsemblBacteria" id="AAZ68656">
        <property type="protein sequence ID" value="AAZ68656"/>
        <property type="gene ID" value="Ecaj_0622"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0622"/>
    <dbReference type="HOGENOM" id="CLU_865286_0_0_5"/>
    <dbReference type="OMA" id="HEHEDSM"/>
    <dbReference type="OrthoDB" id="1672280at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="305" mass="34435" checksum="F5D7BC7FE8E1EDB4" modified="2005-09-27" version="1">MAKLVFDIQTPFKLKNSLFCGFKGFFICNLAAITMLESSLHMIYIRGSRRNTVKQVRAHARYQLKNHIVPFLEVVVVMPLFFILTHFLGDRVFGGFSPTEKYLACLIVCMLVMILICLSLRLLGQRKDPCCGVIRHPIYRSDNKNNNDYIRFRGPNYHTGTSNEPQELSNGLRILLSVVISVLLTPFFLLKAAIELACAAVEVLELPISFLFDLCNLLQDTVTSRTGYHVVRGPKSADRFTHCRKTLNTLSSFLYAGFRDLIVACSLGILNAHIIENHENEHVTPISMLDDAFIGICVFPTTSRT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRL5</accession>
    <name>RS3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsC</name>
        <name type="ordered locus">Ecaj_0606</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a tight complex with proteins S10 and S14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304718.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRL5"/>
    <dbReference type="SMR" id="Q3YRL5"/>
    <dbReference type="STRING" id="269484.Ecaj_0606"/>
    <dbReference type="EnsemblBacteria" id="AAZ68640">
        <property type="protein sequence ID" value="AAZ68640"/>
        <property type="gene ID" value="Ecaj_0606"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0606"/>
    <dbReference type="eggNOG" id="COG0092">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_0_2_5"/>
    <dbReference type="OMA" id="KTNPIGN"/>
    <dbReference type="OrthoDB" id="1132353at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_B">
        <property type="entry name" value="Ribosomal_S3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005704">
        <property type="entry name" value="Ribosomal_S3_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_S3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_S3_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="SSF54821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01009">
        <property type="entry name" value="rpsC_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000230697" description="30S ribosomal protein S3">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="1">
        <location>
            <begin position="38"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <sequence length="211" mass="23856" checksum="5DFA51F52047205B" modified="2005-09-27" version="1">MGQKCNPIGLRLKIINTWDSLWYANKDYTTKLHEDFLLRKFIKKAFYHAAISKVVIARKVDLIMVNVYSAKPGVIIGKKGADIDKVKQQIVKMINNNIELNIIEVKKPELKAVLIAENIAQQLEKRISFRRAMKRSVQNCLKIGAKGIKVSCAGRLGGAEIARTEWYKEGSVPLHTFRANIDYGFSEAKTIYGIIGVKVWVYLGETKSSNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRL7</accession>
    <name>RL29_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmC</name>
        <name type="ordered locus">Ecaj_0604</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304716.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRL7"/>
    <dbReference type="SMR" id="Q3YRL7"/>
    <dbReference type="STRING" id="269484.Ecaj_0604"/>
    <dbReference type="EnsemblBacteria" id="AAZ68638">
        <property type="protein sequence ID" value="AAZ68638"/>
        <property type="gene ID" value="Ecaj_0604"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0604"/>
    <dbReference type="eggNOG" id="COG0255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158491_4_0_5"/>
    <dbReference type="OMA" id="FYCRNIK"/>
    <dbReference type="OrthoDB" id="2063644at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_L29/L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_L29/L35_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="SSF46561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000007478" description="50S ribosomal protein L29">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <sequence length="67" mass="7891" checksum="7A12D854CA44DA83" modified="2005-09-27" version="1">MDIVDIRSKTSDELRELLASLRKELVDAVLNRKIDKSGNHFYCVNIKRDIARVLTVLNERKKEERHV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRL9</accession>
    <name>RL14_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplN</name>
        <name type="ordered locus">Ecaj_0602</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304714.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRL9"/>
    <dbReference type="SMR" id="Q3YRL9"/>
    <dbReference type="STRING" id="269484.Ecaj_0602"/>
    <dbReference type="EnsemblBacteria" id="AAZ68636">
        <property type="protein sequence ID" value="AAZ68636"/>
        <property type="gene ID" value="Ecaj_0602"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0602"/>
    <dbReference type="eggNOG" id="COG0093">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_2_2_5"/>
    <dbReference type="OMA" id="AKEVLCI"/>
    <dbReference type="OrthoDB" id="1799923at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_L14_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_L14P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_L14P_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_L14P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="PTHR11761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="SSF50193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000355814" description="50S ribosomal protein L14">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <sequence length="119" mass="12704" checksum="7002408B05190F23" modified="2005-09-27" version="1">MIQKNTLLDVADNSGARKVLCIGLLNGKKSASVGDVIVVSTKVITPRGKVSKGKVYKAVVVRVKKAVRRLDGSIIRFSSNAVVLINDQGDPLGTRVFGPIKKLPFGLFSKVMSLAVEVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRM3</accession>
    <name>RS8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsH</name>
        <name type="ordered locus">Ecaj_0598</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S5 and S12.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304710.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRM3"/>
    <dbReference type="SMR" id="Q3YRM3"/>
    <dbReference type="STRING" id="269484.Ecaj_0598"/>
    <dbReference type="EnsemblBacteria" id="AAZ68632">
        <property type="protein sequence ID" value="AAZ68632"/>
        <property type="gene ID" value="Ecaj_0598"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0598"/>
    <dbReference type="eggNOG" id="COG0096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_0_0_5"/>
    <dbReference type="OMA" id="FDQGYIL"/>
    <dbReference type="OrthoDB" id="1692188at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_B">
        <property type="entry name" value="Ribosomal_S8_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="SSF56047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000290830" description="30S ribosomal protein S8">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <sequence length="132" mass="14526" checksum="605FC46FEF8702AD" modified="2005-09-27" version="1">MSLSDPIANFLTSIRNGQLSMNKVIVVSYSYVIHAILQILLSEGYIDGFTEKSKSSSIKFFEVKLKYYNGAPVISQIARISKPGKRCYCSAKDMPKFYNGLGLYIISTSKGIMSDYNARKAGVGGEILCGVF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRN2</accession>
    <name>RPOA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit alpha</fullName>
            <shortName evidence="1">RNAP subunit alpha</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoA</name>
        <name type="ordered locus">Ecaj_0589</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase alpha chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRN2"/>
    <dbReference type="SMR" id="Q3YRN2"/>
    <dbReference type="STRING" id="269484.Ecaj_0589"/>
    <dbReference type="EnsemblBacteria" id="AAZ68623">
        <property type="protein sequence ID" value="AAZ68623"/>
        <property type="gene ID" value="Ecaj_0589"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0589"/>
    <dbReference type="eggNOG" id="COG0202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053084_0_0_5"/>
    <dbReference type="OMA" id="LMKFRNF"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00059">
        <property type="entry name" value="RNApol_bact_RpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011773">
        <property type="entry name" value="DNA-dir_RpoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011260">
        <property type="entry name" value="RNAP_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32108">
        <property type="entry name" value="PTHR32108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03118">
        <property type="entry name" value="RNA_pol_A_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="SSF55257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="SSF56553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02027">
        <property type="entry name" value="rpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000225273" description="DNA-directed RNA polymerase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha N-terminal domain (alpha-NTD)" evidence="1">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha C-terminal domain (alpha-CTD)" evidence="1">
        <location>
            <begin position="280"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00059"/>
        </source>
    </evidence>
    <sequence length="372" mass="41393" checksum="18287CF60887D18F" modified="2005-09-27" version="1">MIFDEDSNSVTQDSGYMTVGNAYSNAVGYISMSDHWSKLTKPSSIKVESNGASPNKADLIVEPLESGFALTLGNALRRVMMSSLRGFAVYGVEIENVLHEFTSISGVREDVTDILLNISMMRVKLSGLSNKVLSLRVKGPCEVRSGMIPDTDDCIILNKDLLICTLDQDVDFNIKMYVNSGKGYVPAVKRKSVSKLSDVPVNFIATNALYSPIKKASFKVESSRIGQFTDYDRLVLSVETDGSILPDEAVALAARILQDQFQPFINFDETDEPHKKIDTKDALPYDSNLLRKVDELELSVRSYNCLKNDNITYIGDLVQKTESDMLRTPNFGRKSLNEINELLASMNLHLGMKIANWPPESIESLSKQYSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRP3</accession>
    <name>Q3YRP3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphate ABC transporter ATP-binding protein, PhoT family</fullName>
            <ecNumber evidence="6">3.6.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0578</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of an ABC transporter complex. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.27" evidence="6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304690.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0578"/>
    <dbReference type="EnsemblBacteria" id="AAZ68612">
        <property type="protein sequence ID" value="AAZ68612"/>
        <property type="gene ID" value="Ecaj_0578"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0578"/>
    <dbReference type="eggNOG" id="COG1117">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="TIAMKPE"/>
    <dbReference type="OrthoDB" id="1416748at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="7"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="253" mass="28671" checksum="9CC4367219C48D94" modified="2005-09-27" version="1">MSQQIHASARNLSLWYDTNQILHDINLDIYKREVTAFIGPSGCGKSTFLRCFNRMNDFISNCKIKGSLNVDDICIYSVNTNVVLLRAKVGMVFQKPNPFPKSIYDNVAYGPKLHGLAKNKKKLDEIVERSLTSVGLWEELCDRLKDNAFELSGGQQQRLCIARAIAVRPTMLLMDEPCSALDPVATNVIENLIQNLKKHFTIIVVTHSMKQAKKISDRVAVFDSGRIVEYNTTQEIFENPQSSKTQRYIVDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRP8</accession>
    <name>Q3YRP8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-specific adenosine deaminase</fullName>
            <ecNumber evidence="4">3.5.4.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tadA</name>
        <name evidence="6" type="ordered locus">Ecaj_0573</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>adenosine(34) in tRNA + H(+) + H2O = inosine(34) in tRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:43168"/>
            <dbReference type="Rhea" id="RHEA-COMP:10373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10374"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:82852"/>
            <dbReference type="EC" id="3.5.4.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.33" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304685.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0573"/>
    <dbReference type="EnsemblBacteria" id="AAZ68607">
        <property type="protein sequence ID" value="AAZ68607"/>
        <property type="gene ID" value="Ecaj_0573"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0573"/>
    <dbReference type="eggNOG" id="COG0590">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025810_3_2_5"/>
    <dbReference type="OMA" id="PCQMCAG"/>
    <dbReference type="OrthoDB" id="1890883at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00972">
        <property type="entry name" value="tRNA_aden_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028883">
        <property type="entry name" value="tRNA_aden_deaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14437">
        <property type="entry name" value="MafB19-deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="139" mass="15587" checksum="0E73BD4F6B9945CF" modified="2005-09-27" version="1">MKLAISKAQEDLSEVPVGAVIVYNDEVISSSNNSNIQNTDPTAHAEILAIREACKILSTQILDQCDIYVTLEPCAMCAQAISFAKLRRLYFGAYNKKYGAIENGARIFQFCHHVPEIYGGILENENIKLITDFFKKLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRQ2</accession>
    <name>Q3YRQ2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304681.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0568"/>
    <dbReference type="EnsemblBacteria" id="AAZ68603">
        <property type="protein sequence ID" value="AAZ68603"/>
        <property type="gene ID" value="Ecaj_0568"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0568"/>
    <dbReference type="eggNOG" id="COG0545">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_930494_0_0_5"/>
    <dbReference type="OMA" id="CGQNISA"/>
    <dbReference type="OrthoDB" id="974063at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="303" mass="34832" checksum="7B30231539409801" modified="2005-09-27" version="1">MKKILYRLLYLCLILISVALIGNGLQILYTKAMLNKQDTNNHTKAYKININNSLTHMIGYDLLKPLIEARIDYYIKKNGLVDYLENIKHKIIKPDLFKAYDIADGNGKKVLCGQNISAVVYKFSHNTDSKNIISQTLKNIPQEVTFNLGESIVKELNYTIEGMEEEGQRIIILTDEENNLKEQYYVKLTSINSSYPDEINDMLIFNNTIEDYKGTINKIRCGDSVKVRYDIRESNGKIILENQELKVDIGKNQVPLAIELGIINMRSDMTRSIIASPKLFTNFEKLEHFDENNIKIIDISIIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRQ5</accession>
    <name>YQGF_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative pre-16S rRNA nuclease</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Ecaj_0565</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YqgF nuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304678.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRQ5"/>
    <dbReference type="SMR" id="Q3YRQ5"/>
    <dbReference type="STRING" id="269484.Ecaj_0565"/>
    <dbReference type="EnsemblBacteria" id="AAZ68600">
        <property type="protein sequence ID" value="AAZ68600"/>
        <property type="gene ID" value="Ecaj_0565"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0565"/>
    <dbReference type="eggNOG" id="COG0816">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098240_2_2_5"/>
    <dbReference type="OMA" id="PMGWTAQ"/>
    <dbReference type="OrthoDB" id="2075502at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000967">
        <property type="term" value="P:rRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16964">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00651">
        <property type="entry name" value="Nuclease_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005227">
        <property type="entry name" value="YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317">
        <property type="entry name" value="PTHR33317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03652">
        <property type="entry name" value="RuvX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00250">
        <property type="entry name" value="RNAse_H_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000257531" description="Putative pre-16S rRNA nuclease">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00651"/>
        </source>
    </evidence>
    <sequence length="156" mass="17813" checksum="34569641BE84EE0C" modified="2005-09-27" version="1">MLYKSIDEFVKVIDKKKRIIGLDFGEKKIGIALSDKTNLVAIPYSVYIKRSTRKDLGSLYNIFVENNVGSMVIGWPIELSGIESELCQKVVLLANRIIAKYKINIYLHDERYSTAMATRVAKLANIKRKESQSIDDKIAAMLILQQVLDIMKIYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRQ8</accession>
    <name>Q3YRQ8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metal-dependent carboxypeptidase</fullName>
            <ecNumber evidence="1">3.4.17.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0562</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of a C-terminal amino acid with broad specificity, except for -Pro.</text>
            <dbReference type="EC" id="3.4.17.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M32 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.19" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304675.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68597">
        <property type="protein sequence ID" value="AAZ68597"/>
        <property type="gene ID" value="Ecaj_0562"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0562"/>
    <dbReference type="eggNOG" id="COG2317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032916_1_0_5"/>
    <dbReference type="OMA" id="EFGHALY"/>
    <dbReference type="OrthoDB" id="239313at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001333">
        <property type="entry name" value="Peptidase_M32_Taq"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34217">
        <property type="entry name" value="PTHR34217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02074">
        <property type="entry name" value="Peptidase_M32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006615">
        <property type="entry name" value="Zn_crbxpep_Taq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00998">
        <property type="entry name" value="CRBOXYPTASET"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="491" mass="57062" checksum="13EA2625A35322EA" modified="2005-09-27" version="1">MKHYKFLEQIFDKIRNVNSIISVLESSKNDLSDKIEHVCTLKEIKHEIISNEMVNDMIQHCLADKSKLNEWEIANLNHIELIHKDSVTVPLELKLDLYKAQVKSRNSWLLFRNDNASLQDVIALLSDVVRLVSDVTSIKAESLKTSKYDIILGFQDSKLNTRKVDSIFTEIGAFFRQFIVGISDKQKHSKICYPKDIGEDKQVLFGYDILSCFGITGDNVNVTNQGYINNRYSLTKDLSFLVDYDKSDYRIGIRTLFRKIGYALYNLNLPEKWVKQPVGWNVNSIFSEILGLLTSNHLMLSRECVKYMSTSLKKRFSFRGKVGHYENMQLYFNEVQPSLFMHRSDEVTLLGHIMLRYTLEKEMINDSLQVQDLPDAWVQGMKHYFNIVPKNDLEGFLQDDYWVSGVFGYFPCCMISAIIASQIFCFMKNSNSQILSQVERGDFSYFVSWINKNVCDYGTRYSSLELLKKITGQKLNVNFYKNYLTDKYLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRQ9</accession>
    <name>Q3YRQ9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Transketolase</fullName>
            <ecNumber evidence="3 11">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ecaj_0561</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.</text>
    </comment>
    <comment type="function">
        <text evidence="9">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-N(6)-lipoyl-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + H(+) + pyruvate = (R)-N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10480"/>
            <dbReference type="Rhea" id="RHEA-COMP:10481"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10508"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit. Can also utilize other divalent metal cations, such as Ca(2+), Mn(2+) and Co(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="11">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="3 11"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304674.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0561"/>
    <dbReference type="EnsemblBacteria" id="AAZ68596">
        <property type="protein sequence ID" value="AAZ68596"/>
        <property type="gene ID" value="Ecaj_0561"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0561"/>
    <dbReference type="eggNOG" id="COG0021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_0_0_5"/>
    <dbReference type="OMA" id="HHTEGIE"/>
    <dbReference type="OrthoDB" id="188169at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005478">
        <property type="entry name" value="Transketolase_bac-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033247">
        <property type="entry name" value="Transketolase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43522">
        <property type="entry name" value="PTHR43522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00232">
        <property type="entry name" value="tktlase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4 11 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="12">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="661" mass="72725" checksum="D73551F6E61C156F" modified="2005-09-27" version="1">MGNRYNDMVNMANAIRVLSVDAIQNAASGHPGMPLGMADVATVLFAKFLKFTPKDTSWFNRDRFVLSNGHGSMLLYSILYFLGCLDIEEIKRFRQIHSLTPGHPEYGCTPGVDATTGPLGQGLACAVGMAIAERVLSEKFGSELVDHMTYVMVGDGCLMEGISHEAASLAGHLQISKLIVLFDDNGISIDGDTSLAVSDDIKARFLSYGWDVFEIDGHDFDEIESSIENARRSSKPSLICCKTIIGKGLVSKENTSAAHSWPFSEEEVILMKEKLGWCDKPFDVPEEILQLWKSIASKSENEYKAWLNKCDDKASQSFVDVIKEGVSDSVFLSLKKLKKELCNLNLNEATRKSSGRILEVVMENTSKLIGGSADLTGSNNTKPQFASIINKDNFSGSYLHYGIREHAMAACMNGMALHGGVIPYGGTFLVFADYCRPAIRLSALMKRHVIYVMTHDSIGVGEDGPTHQPVEHVSSLRAIPNLYVFRPADAVEVLECWEIALRLTSSPSAFILSRQNVGNVRSLYVEENLSSKGAYIIREYEKDLDVTIFATGSEVEVALKAADILKGKGLDARIVSVPCWELFAQQDKKYIFSLLNNKSIKAAIEAASSFGWHKYIGEDGVFVGLDDFGLSAPCKDLYKHFGITAENLVNKILDKLKINID</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRR9</accession>
    <name>Q3YRR9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Signal recognition particle subunit FFH/SRP54 (Srp54)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ecaj_0551</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304664.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0551"/>
    <dbReference type="EnsemblBacteria" id="AAZ68586">
        <property type="protein sequence ID" value="AAZ68586"/>
        <property type="gene ID" value="Ecaj_0551"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0551"/>
    <dbReference type="eggNOG" id="COG0541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_5"/>
    <dbReference type="OMA" id="DTAGRHK"/>
    <dbReference type="OrthoDB" id="656414at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004780">
        <property type="entry name" value="SRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF7">
        <property type="entry name" value="PTHR11564:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00959">
        <property type="entry name" value="ffh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54_N" evidence="12">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="10">
        <location>
            <begin position="99"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="SRP54" evidence="11">
        <location>
            <begin position="100"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="449" mass="49425" checksum="D87524E11D50B416" modified="2005-09-27" version="1">MFESLTSSLTNIIQKMQSKHVISSEDFDSTMEEVKIALLDADVALSVVKDFINNVKEKIVGETTVKGVLPTQMIIKKIQDCLIEILGSEKQDLNISAKPFSVIMMVGLQGVGKTTTTAKLALKLKNKKKEVIMASLDLYRPAAQKQLEILGKQIDVKTLPIIENQAPVDIVKRAMEAAQHGNYSVLILDTAGRLHIDQVMMEELKAIKDLSCPSEIILVADSMIGQDAVNVAKSFNDSIGITGVILTRVDGDSRGGAALSMKMSIGSPIKFIATGEKLSDLDDFYPDRIAKRILNMGDVVSLVEKATEAVGKHAIDEMQKKAQKGKFDLNDLVQQLKALNKLGGIANILKFMPNFGSDVKRQVSGIADDSRVKQYIAIVNSMTKKERENPKILNGARKARIAKGAGVQINTINMLLKQFNQMSTLMNKFSKVDYNKLKNVDLMNLFKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRT1</accession>
    <name>Q3YRT1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304652.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68574">
        <property type="protein sequence ID" value="AAZ68574"/>
        <property type="gene ID" value="Ecaj_0538"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0538"/>
    <dbReference type="HOGENOM" id="CLU_619269_0_0_5"/>
    <dbReference type="OMA" id="EDIYFEY"/>
    <dbReference type="OrthoDB" id="1143290at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="442" mass="50485" checksum="ECFFD478C984327B" modified="2005-09-27" version="1">MAGNKKIKSSDLSSLEECSEESSESINNVFHDIEVLELNGNEDEDFRALARIKLEDLEVQESLLELEGEFNKFIVEHVEECGIVEDQEYDHTVVALEDEDFEIEESLLQKILDFFSQLYQDTEDYIDLDYELDPLKRRKKKKKFILEAIIEFLKKLLRSGKSLNLKELLDLQILELQERLAKEEDPDLRKLLQERLILLTELRAQMMQSGIGANLLRFFLLASFISSLLGLQQDAYVTREGTRSRDALGLFVQKQEEKALDEKSVSADASKNIVADHFSSFIVSGYVQDVFLGKVQEMSYKLHDQYFMALRDMSYNIGTQHGMQGVFLAVVAIMSAINAAFSKVTSFFRRAIDKLDAFERSVRSRNNFVNDVHDVKSSASPVVCNTDISFTQSNNLHYSGGIWSSLSYHSELKVSCSGIYNSSYLEDIRVIQCQVQNSSVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRU8</accession>
    <name>Q3YRU8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="3 13">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ecaj_0521</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NAD(+) = (R)-N(6)-lipoyl-L-lysyl-[protein] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11 13">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="13">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="3 13"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304635.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0521"/>
    <dbReference type="EnsemblBacteria" id="AAZ68557">
        <property type="protein sequence ID" value="AAZ68557"/>
        <property type="gene ID" value="Ecaj_0521"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0521"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_1_5"/>
    <dbReference type="OMA" id="DAKYGEW"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11 13" id="KW-0274">FAD</keyword>
    <keyword evidence="4 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 11 13" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 13 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 13" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="15">
        <location>
            <begin position="4"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="14">
        <location>
            <begin position="350"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="147"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="184"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="322"/>
            <end position="325"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="12">
        <location>
            <begin position="42"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAZ68557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="466" mass="50078" checksum="C8BE20CD1763866B" modified="2005-09-27" version="1">MQNYDIVIIGGGPGGYKCAIRASQLGLKVACIDKNKILGGTCLRVGCIPSKALLHFSHEYYHLKNNLSEVGITFDNLNFDLNKIMSFKDKNIAELGSGISQLFSSYKIDYLCGAGKIKSVGSNDFIIAINGDNAEQQITSKNIVIATGSDVSSFPGITIDEENIVSSTAALSFKEPPKRLVVIGAGAIGLEMSSVWSRFGSEVTVVEFLDKIAPSMDGDISKALLTSLKKQCINFKLSTKVVSIDNKGSNLTVHLESVKDGKSEVIEADKVLVSIGRVPYTKGLIDNNLIECDSRGFIKVNSRYETNIPGIFAIGDVIGGAMLAHKAEEEGIAVAELISGHIPHVDYEIIPSVIYTHPAVASIGKTEESLKNANYSYSVGKSNFAANGRSRITNNAVGFVKVLTSKVNNAILGVHIIGTYADTMINEAAIAMAYRASSEDVFRICHSHPDVNEAFRDACEAAFLNH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-10-13" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRX8</accession>
    <name>OBG_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTPase Obg</fullName>
            <ecNumber evidence="1">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP-binding protein Obg</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">obg</name>
        <name type="ordered locus">Ecaj_0490</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304605.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRX8"/>
    <dbReference type="SMR" id="Q3YRX8"/>
    <dbReference type="STRING" id="269484.Ecaj_0490"/>
    <dbReference type="EnsemblBacteria" id="AAZ68527">
        <property type="protein sequence ID" value="AAZ68527"/>
        <property type="gene ID" value="Ecaj_0490"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0490"/>
    <dbReference type="eggNOG" id="COG0536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011747_2_0_5"/>
    <dbReference type="OMA" id="VVFDWEP"/>
    <dbReference type="OrthoDB" id="603226at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01454">
        <property type="entry name" value="GTPase_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="PTHR11702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002401">
        <property type="entry name" value="GTP_bd_Obg/CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="SSF82051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02729">
        <property type="entry name" value="Obg_CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000385899" description="GTPase Obg">
        <location>
            <begin position="1"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Obg" evidence="2">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="1">
        <location>
            <begin position="159"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="165"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="190"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="211"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="278"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="306"/>
            <end position="308"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01231"/>
        </source>
    </evidence>
    <sequence length="340" mass="37810" checksum="89438A4ADFDA36E4" modified="2005-09-27" version="1">MSFIDEAKIYLKAGNGGDGCSSFRREKFIEFGGPDGGNGGNGGNIIFYTSHHINTLLYFRYKQHIKAENGNPGSSKKKSGSSGKDVIIKVPIGTQLYDEDGMLITDLNEENQKFIAAHGGKGGIGNANYKTSTNRAPRHFTFGKRGEEKHIILKLKIISDVGIIGLPNAGKSSFLASCTNSKTKIADYPFTTLEPHLGVAFIDNTELVLADIPGLIPGAHLGHGIGDKFLKHIERCSILLHIIDCTLDNIIESYECIRKELSFYNKELSNKTEFIVLNKSDLLDKKEINQKKQILSNHTKKEIFISSIKNNRYPILSTLIKQIHKKYTNTKPHIYDPFNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRX9</accession>
    <name>ENO_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase</fullName>
            <ecNumber evidence="1">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eno</name>
        <name type="ordered locus">Ecaj_0489</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <text evidence="1">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304604.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRX9"/>
    <dbReference type="SMR" id="Q3YRX9"/>
    <dbReference type="STRING" id="269484.Ecaj_0489"/>
    <dbReference type="EnsemblBacteria" id="AAZ68526">
        <property type="protein sequence ID" value="AAZ68526"/>
        <property type="gene ID" value="Ecaj_0489"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0489"/>
    <dbReference type="eggNOG" id="COG0148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_2_1_5"/>
    <dbReference type="OMA" id="EFMIIPV"/>
    <dbReference type="OrthoDB" id="533698at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <feature type="chain" id="PRO_0000267029" description="Enolase">
        <location>
            <begin position="1"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="1">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="364"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <sequence length="421" mass="46584" checksum="68054444A7ADF022" modified="2005-09-27" version="1">MSNITINKILARQILDSRGYPTIEAEVILSNNTKAKACVPSGASVGKFEAVELRDNDKNYYNGYGVTKAVNIINSEIAPQIIGMNTLNQEKIDNTLIKIDGTDNKSRIGANSTLAVSLAIAKAAASTLNIPLYQYIGGINAKVLPTPLINVINGGMHADNNLDFQEFMIIPNGANKFEDAMRMSAEVFFKLKQILKSKQYNTSVGDEGGFAPNIKTNNEVFEIIIDAIEKSGYKMYKDFSLGLDVAASTFYKDQKYKFADYEFNTQELVEYYKNITSQYPIISLEDPIAEEDTNGWKLITQELGNKIQIVGDDLFVTNCKLIQNGIQNNLANAVLIKPNQIGTLTETFNAIRLAQKNNYNTIISHRSGETEDTTISHIAVAANCGQIKTGSLSRSERLAKYNELLYIEKQLDISAIYYGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS04</accession>
    <name>Q3YS04_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATP-dependent clp protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0464</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304579.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0464"/>
    <dbReference type="EnsemblBacteria" id="AAZ68501">
        <property type="protein sequence ID" value="AAZ68501"/>
        <property type="gene ID" value="Ecaj_0464"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0464"/>
    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_4_2_5"/>
    <dbReference type="OMA" id="GRVIQEH"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="214"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="493"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="ClpB_D2-small" evidence="7">
        <location>
            <begin position="661"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="759" mass="85478" checksum="1013FDE8D7670E2A" modified="2005-09-27" version="1">MLSKNLEASLHKALSIAFDFHHEYATLEHLLLALTDDIDARRVFYAFRVSIDKLKITIINFLRYEIPTLINKDISEVKPNAVFERLVHRAIIHAHTSGKSEITGANILAEILSEKDSYSACFLHEQNVKYIDVMNYTSNNNLYTGEFNIDQEFARDMEYGKPINNVNKDVLKDNEILESYCVNLNEAAKKGKIDYVIGRSYELDRTMEVLLRRRKNNPLYVGDPGVGKTAIVEGLALKIIEGDVPDQLKRMVIYSLDMGALLAGTRYRGDFEERIKSVIKAIEAKENAILFIDEIHTIVGAGSTSGGSLDASNLLKPALARGTLKCIGATTYKEYNNNFEKDRALARRYQKINVEESSVSETLRILDGIKSYYESHHQVRYTNQAIRYAAELSDRYISGKLLPDKAVDVIDEAGVYCKLHNAGSKVITGSDIEHIISRITEIPCSNLSCNDLDRVRNLESNLKQSIFGQDFAISHLVDSIKIAKAGLRNHNKPLASYLFAGPTGVGKTELAKQLAEHMGMKLIRFDMSEYMEPHAISKIIGSPPGYVGYDQGGLLTDSISRHQYSVLLLDEIEKAHNDIYNILLQIMDYGCVTDTYGKKVNFHNVVIILTTNAGAFELSKNSVGFIKNKSFSHGDNEKAIERIFSPEFRNRLDAIISFSSLDQEVMLRVVKKFIFQLKEQLAKKNVHIDVTDDVLMYFAQSGYKDACGVRNIENIISKKLRKYLAEEILFGKLFNGGNVKIKLDSNTNKLIYDFCCAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS07</accession>
    <name>Q3YS07_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transaldolase</fullName>
            <ecNumber evidence="3">2.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0461</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.2.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304576.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0461"/>
    <dbReference type="EnsemblBacteria" id="AAZ68498">
        <property type="protein sequence ID" value="AAZ68498"/>
        <property type="gene ID" value="Ecaj_0461"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0461"/>
    <dbReference type="eggNOG" id="COG0176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079764_0_0_5"/>
    <dbReference type="OMA" id="VRHPMHV"/>
    <dbReference type="OrthoDB" id="784333at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00956">
        <property type="entry name" value="Transaldolase_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033919">
        <property type="entry name" value="TSA/FSA_arc/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="PTHR10683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0704">Schiff base</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="220" mass="24176" checksum="89589C258BB7257E" modified="2005-09-27" version="1">MKLFLDTVDIDFLKEFSITGLIDGVTTNPSLMSKSNLECQDLILKICSLIPGPVSIEVISTSYKDMIKEGLDISKIADNIVVKLPLTYDGLIACKVLSHEHNVKVNVTLCFSVPQAILSAKSGAYFISPFVGRIDDIGQSGMQLIKDIRDVYTSYNTFNTEILVASVRNITHVIEAAKIGADIVTIPPSIFKQMFTHPLTDKGLESFLQSWNASGKKIFS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS22</accession>
    <name>ENGB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">engB</name>
        <name type="ordered locus">Ecaj_0443</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304561.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS22"/>
    <dbReference type="SMR" id="Q3YS22"/>
    <dbReference type="STRING" id="269484.Ecaj_0443"/>
    <dbReference type="EnsemblBacteria" id="AAZ68483">
        <property type="protein sequence ID" value="AAZ68483"/>
        <property type="gene ID" value="Ecaj_0443"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0443"/>
    <dbReference type="eggNOG" id="COG0218">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033732_2_0_5"/>
    <dbReference type="OMA" id="LMMDIRH"/>
    <dbReference type="OrthoDB" id="1746051at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03598">
        <property type="entry name" value="GTPase_YsxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
    <feature type="chain" id="PRO_0000266854" description="Probable GTP-binding protein EngB">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="EngB-type G" evidence="1">
        <location>
            <begin position="26"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="61"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="147"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="176"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <sequence length="200" mass="22732" checksum="223F063C1461C0CB" modified="2005-09-27" version="1">MKLKAIMSKCEFIIGATHVKSFPNFSIPEIAIAGRSNVGKSSLINAITNNKKNAKTSSRPGCTKQINFYLINKSFMVLVDLPGYGYSKATRATINNYLFLMEYYLLNSKNLLKVILLIDVKVGFKEIDLDFINWLELNHIYYQLVLTKIDRVSKEILDVNINYIKNLNLGFVIYPVISASSKYKQGIGELIYEIAQCIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS52</accession>
    <name>Q3YS52_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cysteine desulfurase IscS</fullName>
            <ecNumber evidence="10">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">iscS</name>
        <name evidence="13" type="ordered locus">Ecaj_0410</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0410"/>
    <dbReference type="EnsemblBacteria" id="AAZ68453">
        <property type="protein sequence ID" value="AAZ68453"/>
        <property type="gene ID" value="Ecaj_0410"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0410"/>
    <dbReference type="eggNOG" id="COG1104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_0_2_5"/>
    <dbReference type="OMA" id="APHIINF"/>
    <dbReference type="UniPathway" id="UPA00266"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="PTHR11601:SF34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02006">
        <property type="entry name" value="IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="12">
        <location>
            <begin position="24"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="10">
        <location>
            <begin position="91"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="10">
        <location>
            <begin position="219"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="10">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="10">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <note>ligand shared with IscU</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="422" mass="47185" checksum="1C5C0F930F4A9D86" modified="2005-09-27" version="1">MSLTLSVIGNKMGREGQIHINLPVFLDYQSTTKTDDRVLEAMIPYFQQFSNPHSRSHSFGWKAESAVELARERVASLINAEPKEVIFTSGATESNNLAVKGVANFYKNKGNHIITVRTEHKCVLDSCRHLETEGFNVTYLDVQKNGIINLDLLKSYITDKTILVSVMMVNNEIGVIQPVEEIGKICHEHGIFFHTDAAQAFGKIPIDVNKMNIDLLSISGHKIYGPMGIGALYIRKRQPRVRLTPLINGGGQERGMRSGTIPTPLAVGLGEAARIAQEVMEEENIRIKELRDILYNEIKKHLSYVVLNGDYEQRISGNLNLSFPYVEGESIIMAINNLAVSSGSACTSASLEPSYVLRALNIEKDLEHSSIRFGIGRFTTKEEILYAAELIVSSIKKLREMSPLWEMVQEGIDLNNIKWDTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS53</accession>
    <name>Q3YS53_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Iron-sulfur cluster assembly scaffold protein IscU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0409</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">A scaffold on which IscS assembles Fe-S clusters. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304530.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0409"/>
    <dbReference type="EnsemblBacteria" id="AAZ68452">
        <property type="protein sequence ID" value="AAZ68452"/>
        <property type="gene ID" value="Ecaj_0409"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0409"/>
    <dbReference type="eggNOG" id="COG0822">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079283_5_0_5"/>
    <dbReference type="OMA" id="YKNPRNY"/>
    <dbReference type="OrthoDB" id="1793654at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011339">
        <property type="entry name" value="ISC_FeS_clus_asmbl_IscU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="PTHR10093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01999">
        <property type="entry name" value="iscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NifU_N" evidence="4">
        <location>
            <begin position="3"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="138" mass="14897" checksum="A8400D981615B03B" modified="2005-09-27" version="1">MSYSEVLLEHYKNPKNVGTLPKDDYNVGTGLVGAPSCGDVMKLQIKVDNNGRIIDAKFKTFGCGAAIAASSLATELIKGKTVDEAHQLKNTVLAKELSLPPVKIHCSLLAEDAIKAAINDYNMKQENRKSNTAFSSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS63</accession>
    <name>Q3YS63_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304520.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68442">
        <property type="protein sequence ID" value="AAZ68442"/>
        <property type="gene ID" value="Ecaj_0399"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0399"/>
    <dbReference type="HOGENOM" id="CLU_945730_0_0_5"/>
    <dbReference type="OMA" id="GMPVICA"/>
    <dbReference type="OrthoDB" id="1931903at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="319" mass="35913" checksum="88633C1B66D50DFF" modified="2005-09-27" version="1">MLFSVRKKDDDIVIASNKEKKIALLRMFVKDVRKVAAKSCYGIPKEQQVSLDIFNKPILSLNGTSIVAKSKEHQVIYSEIKSRRETDSEIPYRGYLINVKWFSLKEQYCNTLVIKKIDEFSFGLHSCDGKLYNTSGKRVAYVITLGGNLIIHDHIVCDRSKGQTYFHSTLAAGMPVICAGLIRVDNGMIRDISNESGHYKPGIANLYNAVKLLDNVICLDCMIRIVSVTINSVTKESQIFYKVRMKGSFLADMEAVGEDGLTIPQRCFSLLKRCDDNYQKIVNNALIKNNTGCMDNVLVNVSVRIHVLITVYVAMIIIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS67</accession>
    <name>TSAD_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="1">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tsaD</name>
        <name type="synonym">gcp</name>
        <name type="ordered locus">Ecaj_0395</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304516.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS67"/>
    <dbReference type="SMR" id="Q3YS67"/>
    <dbReference type="STRING" id="269484.Ecaj_0395"/>
    <dbReference type="EnsemblBacteria" id="AAZ68438">
        <property type="protein sequence ID" value="AAZ68438"/>
        <property type="gene ID" value="Ecaj_0395"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0395"/>
    <dbReference type="eggNOG" id="COG0533">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_0_2_5"/>
    <dbReference type="OMA" id="HLEGHIY"/>
    <dbReference type="OrthoDB" id="1257362at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="PTHR11735:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03723">
        <property type="entry name" value="T6A_TsaD_YgjD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000303352" description="tRNA N6-adenosine threonylcarbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="137"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01445"/>
        </source>
    </evidence>
    <sequence length="350" mass="38212" checksum="AF9AF2EFCDA9416B" modified="2005-09-27" version="1">MKKSVKVVLGIETSCDETAVAIVNSNKEVLSHKILSQKEHAEYGGVVPEIASRAHINYLYDLTVSCIEESQLSLNNIDAVAVTSGPGLIGGLIVGVMIAKGIASVTGKPIIEINHLEAHALIVRMFYEINFPFLLLIISGGHCQFLIVYNVGCYHKLGSSLDDSLGEVFDKVAKMLNLGYPGGPVIEKKSLSGDSKSFVLPRALTGRCGCDFSFSGLKTAVRNIIMNHEYIDNKLICDISASFQECVGDILVNRINNAIAMSKAIDKRIDKLVVTGGVAANKLLRERMLRCASDNNFEIFYPPSKLCTDNGIMIGWAGIENLVKDYVSNLDFAPKARWPLESLRSNIMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS69</accession>
    <name>Q3YS69_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Triosephosphate isomerase</fullName>
            <shortName evidence="5">TIM</shortName>
            <shortName evidence="5">TPI</shortName>
            <ecNumber evidence="5 6">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tpiA</name>
        <name evidence="8" type="ordered locus">Ecaj_0393</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-erythrulose 1-phosphate = D-erythrulose 4-phosphate</text>
            <dbReference type="Rhea" id="RHEA:49588"/>
            <dbReference type="ChEBI" id="CHEBI:58002"/>
            <dbReference type="ChEBI" id="CHEBI:90796"/>
            <dbReference type="EC" id="5.3.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 6">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 6">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; erythritol degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="5 6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68436.1"/>
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        <property type="term" value="P:glycolytic process"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
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    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="23"/>
            <end position="25"/>
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    <feature type="active site" description="Electrophile" evidence="5">
        <location>
            <position position="108"/>
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    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="175"/>
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    <feature type="binding site" evidence="5">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000148"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004939"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="255" mass="28016" checksum="E773F387D74475B2" modified="2005-09-27" version="1">MSSSLIAGTLFLVIFMSLLIVANWKMYGDFITFSSFTKELSINLTNVKADLEVVLCPPFIALPKVVDCISCIKFGAQNCFYETEGKYTGEVSAKMLYDCGCSYVIVGHSERRSIFHESDYHVQLKASAAIKSGLIPIVCVGETLLDRENGMLKDSLLNQCYNSFPKSGKFIIAYEPVWAIGSNTIPSIDMITEALEVIRSYDSTSDIIYGGAVNQDNIRDVIAINQLSGVLVGSASLKLDSFYNIIHGAVNVRQN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS74</accession>
    <name>ATPG_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase gamma chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector gamma subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase gamma subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpG</name>
        <name type="ordered locus">Ecaj_0388</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase gamma chain family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAZ68431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
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    <dbReference type="SMR" id="Q3YS74"/>
    <dbReference type="STRING" id="269484.Ecaj_0388"/>
    <dbReference type="EnsemblBacteria" id="AAZ68431">
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        <property type="gene ID" value="Ecaj_0388"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="1701531at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PTHR11693"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ATPsyn_F1gamma"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000053206" description="ATP synthase gamma chain">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00815"/>
        </source>
    </evidence>
    <sequence length="281" mass="32323" checksum="DC9EC36A6E16E558" modified="2005-09-27" version="1">MANLKALLLRIKSVKSIQKTTKVMQMISAAKLHRVQQKLENARKHLLEISNVIDSVDKDNVSDVFSKHKKEKVLLVIMSSDRGLCGNFNNLIVKFTKSYIEDLESDNKEVNLLFFGKKAYDMMYSQYSDKILNVLPDVKSITNFLYFKLFVYSSGVNFEKFDNVIVLFNKFYSTILQKPSAQELIPCNVEMSVLLKEMYQYEPTYIDVISTISLGYILNLMYIAFLENSASEHCSRMIAMESANRNTKDMLNKLALEYNRSRQASITTDLIEIISGFESLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS75</accession>
    <name>Q3YS75_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0387</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304508.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0387"/>
    <dbReference type="EnsemblBacteria" id="AAZ68430">
        <property type="protein sequence ID" value="AAZ68430"/>
        <property type="gene ID" value="Ecaj_0387"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0387"/>
    <dbReference type="eggNOG" id="COG0666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_223858_0_0_5"/>
    <dbReference type="OMA" id="DQMFAHG"/>
    <dbReference type="OrthoDB" id="7148at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="SSF48403"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="2575"/>
            <end position="2607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1993"/>
            <end position="2015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2111"/>
            <end position="2144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2158"/>
            <end position="2186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2848"/>
            <end position="2957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3256"/>
            <end position="3326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3341"/>
            <end position="3562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4055"/>
            <end position="4084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4221"/>
            <end position="4245"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="302"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="731"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2848"/>
            <end position="2913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="2914"/>
            <end position="2957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3263"/>
            <end position="3305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="3355"/>
            <end position="3395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
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            <end position="3414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="3464"/>
            <end position="3504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="4059"/>
            <end position="4078"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="4245" mass="471383" checksum="B80B0D7806152AB1" modified="2005-09-27" version="1">[encoded data, 4245 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS84</accession>
    <name>Q3YS84_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0378</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0378"/>
    <dbReference type="EnsemblBacteria" id="AAZ68421">
        <property type="protein sequence ID" value="AAZ68421"/>
        <property type="gene ID" value="Ecaj_0378"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0378"/>
    <dbReference type="HOGENOM" id="CLU_128099_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="209" mass="24587" checksum="7E21B5CC9629B55F" modified="2005-09-27" version="1">MLLPHKLKVKAIYHHIDRSDNIKELLYTYKKLIKIKKYLYKTKKIYKKINIISIKKKLCKEHKKNNLSIYIKKEIKKEIKTLLMSTIAHEMDPIQRPGQTVDSNIRGARKFGRSHKVKKKTRNKSKSKTIFLKLLNFLFNLIVPMPSCVLSADLIIKNCIIKNLPYIIHKKNHKNSKDNNNKPNKTTIDKPLKHISISQHTDKYYSIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSA7</accession>
    <name>Q3YSA7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304476.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68398">
        <property type="protein sequence ID" value="AAZ68398"/>
        <property type="gene ID" value="Ecaj_0355"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0355"/>
    <dbReference type="HOGENOM" id="CLU_1319261_0_0_5"/>
    <dbReference type="OrthoDB" id="2129507at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="208" mass="22891" checksum="428C6D436523EA18" modified="2005-09-27" version="1">MSDSGIGNRILVRPSKYSSFRPLCFPSVLFSGGGKALVCVKCSLSSSTILADPEWNDVIGLSKLRSLWKCNIKFSVYILVGEKQMLSLCSVVASGEKFSFLKENLGNYGEVILSKEGCYKTARQEAMDLVNVAGLNAEFILPVDSTADTAECSVPVENEFGYVYEKSSATRFQSEALPLKLEEDLLMEVQKKVIVVVLQCVKKNKSYV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSA8</accession>
    <name>HEM3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Porphobilinogen deaminase</fullName>
            <shortName evidence="1">PBG</shortName>
            <ecNumber evidence="1">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylbilane synthase</fullName>
            <shortName evidence="1">HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemC</name>
        <name type="ordered locus">Ecaj_0354</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="1">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304475.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSA8"/>
    <dbReference type="SMR" id="Q3YSA8"/>
    <dbReference type="STRING" id="269484.Ecaj_0354"/>
    <dbReference type="EnsemblBacteria" id="AAZ68397">
        <property type="protein sequence ID" value="AAZ68397"/>
        <property type="gene ID" value="Ecaj_0354"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0354"/>
    <dbReference type="eggNOG" id="COG0181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_0_2_5"/>
    <dbReference type="OMA" id="LWQANHI"/>
    <dbReference type="OrthoDB" id="1450400at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018160">
        <property type="term" value="P:peptidyl-pyrromethane cofactor linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PTHR11557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="SSF54782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000059096" description="Porphobilinogen deaminase">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="1">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00260"/>
        </source>
    </evidence>
    <sequence length="298" mass="32902" checksum="B8052F26F30624FE" modified="2005-09-27" version="1">MNIRIGTRGSVLALAQTLEVTNLLNRYFPEINIQVVKIKTSGDINNKVPINTIGGKGLFIKEIEEALLIGKVDLAVHSVKDIPAFYCDGLIIPCVLKRSSPYDVFVSSKYQSIKSLPINATVGTSSIRRKVQLNYLRPDLQVIPVRGNIDTRILKSDIGEFDGIVLAEAGLIRINKCNVIKEVLDSKIMLSAVGQGAICVQCRADDYNIINKIKVLNCHKSYVCVIAERSFLKTINGSCDTPLAALAQYVDSDTIYMSCMLSNEKEMVFSDCYFKECNAEESGIDMGKKLINELYGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSC9</accession>
    <name>Q3YSC9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0333</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible conversion of aspartate and 2-oxoglutarate to glutamate and oxaloacetate. Can also transaminate prephenate in the presence of glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoglutarate + L-arogenate = L-glutamate + prephenate</text>
            <dbReference type="Rhea" id="RHEA:22880"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58180"/>
            <dbReference type="EC" id="2.6.1.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304454.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0333"/>
    <dbReference type="EnsemblBacteria" id="AAZ68376">
        <property type="protein sequence ID" value="AAZ68376"/>
        <property type="gene ID" value="Ecaj_0333"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0333"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_5"/>
    <dbReference type="OMA" id="SVAMTGW"/>
    <dbReference type="OrthoDB" id="554560at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033854">
        <property type="term" value="F:glutamate-prephenate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="4">
        <location>
            <begin position="32"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="398" mass="44006" checksum="BBBF24557F572E48" modified="2005-09-27" version="1">MSLIAGRMDCIKPSPTLEIASQAQKLKMSGVDVISLSAGEPDFDTPQHVKQAAIDAINAGKTKYTAVDGIIELKKAIVDRFKQDHDLTYDVNQVSVGNGAKQCIYNLLMATINDGDEVIIPAPYWVSYPDVVKISGGNPVIVDCSETFKLTPDLLESVITEKTKWLIINSPNNPTGLMYTYEELQCIAEVLLKYPNIYIMTDDIYSKIVYDGFKFFTIAQVEPRLYDRVFTVNGVSKAYAMTGWRIGYVAGDSKVISAISVIQSQSTTNPNSIAQFASVHALTGDQEFLKERNKIFAERRDMMMNMVNNTSVLSVKKPQGAFYVFISCKELIGKSAKNGLVINSGIDFTRYLLEDYNVAAVPGEAFGVQGFFRISYATSTEQLSKACDRILDACNKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSD0</accession>
    <name>Q3YSD0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alkyl hydroperoxide reductase C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0332</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304453.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0332"/>
    <dbReference type="EnsemblBacteria" id="AAZ68375">
        <property type="protein sequence ID" value="AAZ68375"/>
        <property type="gene ID" value="Ecaj_0332"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0332"/>
    <dbReference type="eggNOG" id="COG0450">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_21_1_5"/>
    <dbReference type="OMA" id="WWPKDFT"/>
    <dbReference type="OrthoDB" id="892697at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="2"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="7">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="205" mass="23322" checksum="87B4EBCD430ABDEF" modified="2005-09-27" version="1">MNLITKQAIDFTAKAVMPDDKICEFNLKEYVNGKCAILVFYPRDFTFVCPTEIISLHNRIPQFMEKNTEIIGISTDSEFCHSHWRNVSVNKGGIDKISFPLIADDSHFISSSYGVLHNNSIALRATFIIDDKFIVRHQSINDLPIGRNVDELLRIIDAIKHYQIHGEVCPAGWMKGDPAMEASQHGIEDYLKSHIDNLEKSIMDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-05" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSD4</accession>
    <name>SYP_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proline--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="1">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proS</name>
        <name type="ordered locus">Ecaj_0327</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304449.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSD4"/>
    <dbReference type="SMR" id="Q3YSD4"/>
    <dbReference type="STRING" id="269484.Ecaj_0327"/>
    <dbReference type="EnsemblBacteria" id="AAZ68371">
        <property type="protein sequence ID" value="AAZ68371"/>
        <property type="gene ID" value="Ecaj_0327"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0327"/>
    <dbReference type="eggNOG" id="COG0442">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016739_4_2_5"/>
    <dbReference type="OMA" id="NCDYAAN"/>
    <dbReference type="OrthoDB" id="665824at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00861">
        <property type="entry name" value="ProRS_anticodon_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00779">
        <property type="entry name" value="ProRS_core_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01570">
        <property type="entry name" value="Pro_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004500">
        <property type="entry name" value="Pro-tRNA-synth_IIa_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023716">
        <property type="entry name" value="Prolyl-tRNA_ligase_IIa_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044140">
        <property type="entry name" value="ProRS_anticodon_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033730">
        <property type="entry name" value="ProRS_core_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00409">
        <property type="entry name" value="proS_fam_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000248897" description="Proline--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01570"/>
        </source>
    </evidence>
    <sequence length="424" mass="48616" checksum="D5665CA5C8E6B0B4" modified="2005-09-27" version="1">MRLSNYYIPTLKETSADVSVVSHKYSIRAGLIKQIASGIYTWLPLGLRVLRNIENIVREEMNKSGALEVLMPLIQPASLWKESGRYDDYGSEMLRITDRNQREMLFGPTHEEVITDTLRTTLTSHKNLPITLYQIQWKFRDELRPRYGIMRCREFLMKDAYSFDKDLSGAILSYNLMFKTYIKIFKNLGLTPIAVKAESGPIGGNLSHEFHVLANSGESTLYYDQEIIELMNSDNIDIERIKNTYTAADDMHDPQTCPIPPNNIKTSKGIEIGHIFYLGDKYSKPMNANFCDNNDSKPLQMGCYGIGISRLVAAIIEVFHDNKGITWPEEISPFKFSLVNLYSSNDKCIKVAENLHMQLHNDVLYDDTDDSPGIKLTRTDLLGMPWQIIIGKSTVEQDLIEVRNRSNKDKFLISVEQFLKKFKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSF3</accession>
    <name>SYC_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="1">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cysS</name>
        <name type="ordered locus">Ecaj_0308</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304430.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSF3"/>
    <dbReference type="SMR" id="Q3YSF3"/>
    <dbReference type="STRING" id="269484.Ecaj_0308"/>
    <dbReference type="EnsemblBacteria" id="AAZ68352">
        <property type="protein sequence ID" value="AAZ68352"/>
        <property type="gene ID" value="Ecaj_0308"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0308"/>
    <dbReference type="eggNOG" id="COG0215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_0_1_5"/>
    <dbReference type="OMA" id="AKYWMHN"/>
    <dbReference type="OrthoDB" id="952207at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="PTHR10890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000240910" description="Cysteine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="269"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <sequence length="459" mass="52382" checksum="A123D98AA1658EE0" modified="2005-09-27" version="1">MIIYNTLTGIKEPFIPLNVDNIKVYVCGPTVYNFVHIGNARSIVIYDVLFRLLKLLYPSVTYVRNITDIDDKIINVAQSNGQNICEVTSVYIKAFHEDMKMLNCLEPTYEPKATDNINVMINLIQKLIDHGHAYVDNATVFFNVESYPSYGQLSKRNIKELIYGSRVDIELGKKHPGDFVLWKPATEVDNKLMSCWPSPWGLGRPGWHIECSAMSYCYLSENFDIHGGGADLQFPHHENEIAQSCCAFPGSYYAKYWIHNGFLTVNGEKMSKSLGNVLTVRQLLESGVKGELIRYVLLNTHYRKPLDWFNMTLVSAQESLNRMYTALSSVNVDLSLDDDVELSSDVVDCLKDDINTPKAISVLHEMVTEINKTSDVMKKTSLAKTLIKTANFLGILQHSWQDWFRVNENDQEIDKLIDERRIARANNDFKKADDIRQLLLSKGIVLSDNKDGTTHWYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSJ9</accession>
    <name>Q3YSJ9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304384.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0259"/>
    <dbReference type="EnsemblBacteria" id="AAZ68306">
        <property type="protein sequence ID" value="AAZ68306"/>
        <property type="gene ID" value="Ecaj_0259"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0259"/>
    <dbReference type="HOGENOM" id="CLU_751712_0_0_5"/>
    <dbReference type="OMA" id="FAAWQEQ"/>
    <dbReference type="OrthoDB" id="1095176at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="368" mass="40808" checksum="9F8F62C77FD7A5C5" modified="2005-09-27" version="1">MFGFLKKGASVIIKAAVTPTTSKLPHQESVGKHLEGMLKSIVPGQKSSREKNFDLKMHDRTYKLGVELPGRTRVAGHETEVALKVPSYKLPYQALQKFAAWQEQNKEGEVDETRKDALASLITPVAATRELIASKTADQYFQKLKELDSQLKIICGKVTLDSTAGSYKQGLTVELDVSGKSKEQIEQEVKIVLQSLGITDSKLAKTFAQRLYKTSTDPKVSSADIQDKKTNTDSKDTTDDKDVVSSAPAQNSDVVSPKKEQRSDLYPAPESSTMSDSDFSLTNPRNMPYNTRGPLQSSEMLQKLQQQILMQHRPEPLFPQPMSPSMQQAVEEIARDLPTNINEEEHMQGNSTNTGIPIHNTGKAHARR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-05" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSK6</accession>
    <name>MIAB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase</fullName>
            <ecNumber evidence="1">2.8.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-i(6)A37 methylthiotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">miaB</name>
        <name type="ordered locus">Ecaj_0252</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-SH + AH2 + N(6)-dimethylallyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37067"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="Rhea" id="RHEA-COMP:10376"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="ChEBI" id="CHEBI:74417"/>
            <dbReference type="EC" id="2.8.4.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylthiotransferase family. MiaB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304377.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSK6"/>
    <dbReference type="SMR" id="Q3YSK6"/>
    <dbReference type="STRING" id="269484.Ecaj_0252"/>
    <dbReference type="EnsemblBacteria" id="AAZ68299">
        <property type="protein sequence ID" value="AAZ68299"/>
        <property type="gene ID" value="Ecaj_0252"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0252"/>
    <dbReference type="eggNOG" id="COG0621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_2_0_5"/>
    <dbReference type="OMA" id="CEHFHIP"/>
    <dbReference type="OrthoDB" id="397139at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035596">
        <property type="term" value="F:methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01864">
        <property type="entry name" value="tRNA_metthiotr_MiaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006463">
        <property type="entry name" value="MiaB_methiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00273">
        <property type="entry name" value="(dimethylallyl)adenosine_tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000374272" description="tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase">
        <location>
            <begin position="1"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="143"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="441" mass="50164" checksum="36184C98734090F9" modified="2005-09-27" version="1">MKGLYIKSYGCQMNVYDSLIMENIIKPLGFTVVSEPSEADVVILNTCHIREKASEKLYSELGKIRKIQENKDLTIVVAGCVAQAEGEEIFERSPFVDIVVGPQSIHTLPELLVKAHRIKKQVINIDFPVISKFDAIPVEEYTKNQETSAFISVQEGCNKFCTFCVVPYTRGEEYSRTVEAIFNEALVLADSGIKEITLIGQNVNAYHGTYKGCEWDLGKLIQHIAKIPNIERIRYTTSHPRDMHQSLYEAHKLEEKLMPFVHLPVQSGSDRILKKMNRKHTAEEYIDIISHLRKQRPDIAFSSDFIVGFPGETEEDFENTIKLVEKVKFSQAYSFKYSPRPGTPGAEYPNQVPEEIKSERLLRLQKLLREQQLAFNRSMIGETCTVLFSSKKGKFDNQIIGKTAYMQSCYINTDNPSQFYNSISPIKIIDAHQNSLTGVVC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-03-20" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSK9</accession>
    <name>ZNUC_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zinc import ATP-binding protein ZnuC</fullName>
            <ecNumber evidence="1">7.2.2.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">znuC</name>
        <name type="ordered locus">Ecaj_0249</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex ZnuABC involved in zinc import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP(in) + H2O(in) + Zn(2+)(out) = ADP(in) + H(+)(in) + phosphate(in) + Zn(2+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29795"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.20"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (ZnuC), two transmembrane proteins (ZnuB) and a solute-binding protein (ZnuA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Zinc importer (TC 3.A.1.15.5) family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAZ68296" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="7.2.2.20" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68296.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_044261955.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSK9"/>
    <dbReference type="SMR" id="Q3YSK9"/>
    <dbReference type="STRING" id="269484.Ecaj_0249"/>
    <dbReference type="PRIDE" id="Q3YSK9"/>
    <dbReference type="EnsemblBacteria" id="AAZ68296">
        <property type="protein sequence ID" value="AAZ68296"/>
        <property type="gene ID" value="Ecaj_0249"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0249"/>
    <dbReference type="eggNOG" id="COG1121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_5"/>
    <dbReference type="OrthoDB" id="1721927at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015633">
        <property type="term" value="F:ABC-type zinc transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017882">
        <property type="entry name" value="ZnuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42734:SF9">
        <property type="entry name" value="PTHR42734:SF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51298">
        <property type="entry name" value="ZNUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0864">Zinc transport</keyword>
    <feature type="chain" id="PRO_0000281500" description="Zinc import ATP-binding protein ZnuC">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="24"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="56"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="242" mass="27691" checksum="1E17C3C6792E1290" modified="2007-03-20" version="2">MFHRLLNKSKILACDDKSVNDYIINVKNLSFFYSKKKVIDNISFRVKFGEIITILGPNGGGKTTLIRILVGIYKNYVGLVEYAKDFTIGYLPQHFSVNSLIPMTVEYFLNSSYTNRKRKLGVGDVLKDVNIEKILDRQMSEISYGELQLVLLARCLMLNPDLIILDEPVSCMDINAKNSFYKLINKLISIYNLSVIMTSHDLHFVMSNSYRVICINKSVYCEGSPSEIVKNEKFLKMFSSYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSL5</accession>
    <name>Q3YSL5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutathione S-transferase, N-terminal</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0241"/>
    <dbReference type="EnsemblBacteria" id="AAZ68290">
        <property type="protein sequence ID" value="AAZ68290"/>
        <property type="gene ID" value="Ecaj_0241"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0241"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_5_3_5"/>
    <dbReference type="OMA" id="ERVYKLQ"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="87"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="225" mass="26516" checksum="65BE35B54009CB2C" modified="2005-09-27" version="1">MKLTMDTLYHFPLCPFSRKVRIFLKEKKFNFHQIEENPWKKREGFIKINPVCQVPVLISGQHVIADSQAICEYIEELYDSASLLGSSLYTRSIVRKLIYWIDYKFYHEITRYIINEKISKYYIKNASPDSRFIQAARQNLLPHVRYIERLLNASSWIACNEFTLADITLAAHISVLDFMNIFPWHLTATLKEWYSVIKSKPSFSDILNDKVIGITPPVHYNKLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSL7</accession>
    <name>Q3YSL7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304366.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68288">
        <property type="protein sequence ID" value="AAZ68288"/>
        <property type="gene ID" value="Ecaj_0239"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0239"/>
    <dbReference type="HOGENOM" id="CLU_309668_0_0_5"/>
    <dbReference type="OMA" id="TRTHSEY"/>
    <dbReference type="OrthoDB" id="633542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="685"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="857"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="907"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="951" mass="108055" checksum="9C05D610388FA0E7" modified="2005-09-27" version="1">MEPRKILTSNQDKLYKQILNLIKKYRATHRTFLRSPLRYCIDQIRFLAFNRKNNSHESSIVITEALTEIVILQSITFEDFAAVLEALELDFRYTLEEAGVSVLFTLFYGLIGHISDQMCNTGEAVHSLQSITDILRSCPQFSHTFDPRNIACYDQFLSRIRALQNFDIETLAEEARIVLSTDAPLSLQTVLLRGLAHAILRQADPESHVDHGEQIDIEASTAIRLVSTAILYQTRERHVAEARAAYAVAHPNRHTAFTTAIRWNERLAFISPAYACAVLSTFFAREFSEFVVNRARDDLRTRFNITFSLNHTREYSSTVNTVLNNTTNNATVFVPTHSSEYFPEWNGLANGIPSYFTTLLSCIQLAFALPPTVLGIHRRVFIRRYLENHNPTREEILNDLTFQSILLFRWFGNLALQPADCISHAVKDAIFKWSIFRYFTPSSAIEEPCKFEYVHNVRQRFSSRNARRFRTDTMFSLGTAGTILALEYTDVALFNFKNIGFTFFVKFARTLGAIRPIFMNRIHGNMLYNPRLESLVITNLVRITGTVPLLPLYDTFGAQLRYMNLLTAIDVIVTSNVDARSSRLFNECTILEEHLTAIENVTQNVLEYRGALALLAPELHRRFDDARYIIPISILLEEDYKEAQKKRASRKPRYSISQDTTTLSTEQLEQEIASANEEGVSLLGMRPLESSQQTPTTESTEQELSSSYKYTFLHQSRQDFSSEPERRKARKPLHKKQPKSIYTGEGSQQDKTKQQKKKKLSPDTQNVQLLSHIAQTDDIQVEDETFTIPDMPQQPQPQPSPRLLSFLEFAQADLQALEQHIQLSTDAQRICTTQQQSALTTQHLTTNVMHHAARESYIQSTQDQQQPNDNQLSSTQTHVPASGIPGVTLISSTRRSSTVSVHSLSPIFVQQSTGSNEGENTPSEDDTNPSSQVALASISGVSQGQFHQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSM2</accession>
    <name>Q3YSM2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA gyrase subunit A</fullName>
            <ecNumber evidence="8">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gyrA</name>
        <name evidence="10" type="ordered locus">Ecaj_0234</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304361.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0234"/>
    <dbReference type="EnsemblBacteria" id="AAZ68283">
        <property type="protein sequence ID" value="AAZ68283"/>
        <property type="gene ID" value="Ecaj_0234"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0234"/>
    <dbReference type="eggNOG" id="COG0188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002977_6_1_5"/>
    <dbReference type="OMA" id="THHWLLF"/>
    <dbReference type="OrthoDB" id="217468at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034335">
        <property type="term" value="F:DNA negative supercoiling activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01897">
        <property type="entry name" value="GyrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005743">
        <property type="entry name" value="GyrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="SSF101904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TOP4c" evidence="9">
        <location>
            <begin position="10"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GyrA-box" evidence="8">
        <location>
            <begin position="550"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="8">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="898" mass="100266" checksum="409FE47A6A00B3E5" modified="2005-09-27" version="1">MSDLNNVISVSIEQELEDSYLSYAMSVIISRAIPDIRDGLKPVHRRILYSMYKSGYDYGKPYKKAARVVGDVMGKYHPHADAAIYDSLVRMAQDFSLLLPLIDSQGNFGSIDGDPPASMRYTEARLARAAHFLLNDIDEDTVDFRPNYDENEVEPVVLPAEFPNILVNGAGGVAVGMSTNIPSHNLGEVVDACIAYIDNPEITFDELINIVRGPDFPTGGTILGDAGIRSAFMTGKGTIIVQGKTHLEDLPSGKQAIIIDEIPYQVNKAKLIERIHELVKEKKIEGILDLRDESNKMGIRVAIELKKQVSHQVVLNQILGLTPLRTTFSINTLVLENNKPKVMSLLDIISTFVEYRREVLTRRTQFRLRKVREKAHLYIGMYIAVLNIDKVISIIRSSRDTAEATKRLLECKWDALTSVREMIELVADTKSLVVDDVCQLTGVQVKSILEMKLQRLTNLEKEKLELDLKSMVSMIRQYTELLKNKEALLKLIKDGLIDVKTKLAVPRRTTIDYSHFETDVEDLIPKEDMVVTVTMSGYIKRAKLSNYRAQRRGGKGRIGQAIKEEDVITNLFVVNTHTSVLFFSNIGRVYKLKVYKLPLGEPTSRGRSLVNIFPLSENESITSVMSLPDNVEDAESLNIVFATASGNLRRNALADFQYVPSNGKIAMVLDEGDTLISVKVCKEEDHVLLSTRLGKSIRFSVSNVRQFKGRMSDGVRAIKLSSQDNVISMSILLAMDIGSEKKDLYLKVPLDKRIAVKEEGFIGTGIVKILADLGLDQDTFMTMVQNEQFILTVTENGFGKRTSAYEYRTTLRGGVGVVNILTTTRNGNVVASFPVESNDHIMLITDKGKLIRIAVSDIRIIGRSTQGVTLFKTEKHEKVVSVAKIIDAKDDSEIDDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSM5</accession>
    <name>Q3YSM5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304358.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68280">
        <property type="protein sequence ID" value="AAZ68280"/>
        <property type="gene ID" value="Ecaj_0231"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0231"/>
    <dbReference type="HOGENOM" id="CLU_841287_0_0_5"/>
    <dbReference type="OMA" id="ENRESTK"/>
    <dbReference type="OrthoDB" id="1473471at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="328" mass="38471" checksum="6E758E496952E58A" modified="2005-09-27" version="1">MYNTILIISITTALIILTITLLFIKNMNKRSNKLFFDKQLTNISRDFIQTIFYPTNYEQQNITNPFHNIFSKRLSISIYDLDDRLFEQFPKVLQSYQLKNTSNPYINKHFQYISQILKIRKTDNNFKQYRHIIALAATLDEMFVKFNNSEIHPDIIHEIVYHFYQNTYAQATIKILNNIFNVGKKLTHIIKAKSTHILFRVYNDNLINISINTYISLSEVTTPNKIHNFNATLSFDISCTKNSKTCDSTISYNYTSLNLLIPNSMKQYTTQEKITVEDALFSNIIQNPNYATLNYNNQHEYNNDILLLNYCIPNPTVESLFKDKSQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSM9</accession>
    <name>Q3YSM9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pilus retraction ATPase PilT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0227</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304354.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68276">
        <property type="protein sequence ID" value="AAZ68276"/>
        <property type="gene ID" value="Ecaj_0227"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0227"/>
    <dbReference type="eggNOG" id="COG2805">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822955_0_0_5"/>
    <dbReference type="OMA" id="TAITCIN"/>
    <dbReference type="OrthoDB" id="473670at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="336" mass="38556" checksum="CA45E4955FBB0D0E" modified="2005-09-27" version="1">MLLFKQLIQSSIGYSSNYLYLTPNNPPAIRRFNNIEFLHTKSLLKEEIEEIIKSILKAEEYKIYMAQKFFNTTLNFNDSHQLYIYISHTSQSFKITVDIIINELTLAPEFEKELNRIISIYSKGLILFTGNICNTNTLLSILHQINNNQKKHVLVFTNNKMSIQKANKALISIYNLSQAESTFIKNQSADIVLFSNFNNINLVNTAIMCINMGLSVIAFTDTISCSCALEGISALFSHKKSILKILSTHLVAIITQVFINKVSIDNLHLYEIIECDTTIQQFIREDNLSEIKNSGIIKNINELIEKHQIHYHDVHNILKNLSDTKLFTEPDLNDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSN8</accession>
    <name>Q3YSN8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">3-oxoacyl-[acyl-carrier-protein] synthase 2</fullName>
            <ecNumber evidence="3 10">2.3.1.179</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ecaj_0218</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor. Can efficiently catalyze the conversion of palmitoleoyl-ACP (cis-hexadec-9-enoyl-ACP) to cis-vaccenoyl-ACP (cis-octadec-11-enoyl-ACP), an essential step in the thermal regulation of fatty acid composition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(9Z)-hexadecenoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxo-(11Z)-octadecenoyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:55040"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10800"/>
            <dbReference type="Rhea" id="RHEA-COMP:14074"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:83989"/>
            <dbReference type="ChEBI" id="CHEBI:138538"/>
            <dbReference type="EC" id="2.3.1.179"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 12">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.179" evidence="3 10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304345.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0218"/>
    <dbReference type="EnsemblBacteria" id="AAZ68267">
        <property type="protein sequence ID" value="AAZ68267"/>
        <property type="gene ID" value="Ecaj_0218"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0218"/>
    <dbReference type="eggNOG" id="COG0304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_69_2_5"/>
    <dbReference type="OMA" id="QIGHCLG"/>
    <dbReference type="OrthoDB" id="606297at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="PTHR11712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="B_KETOACYL_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_KS" evidence="13">
        <location>
            <begin position="6"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAZ68267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="422" mass="44852" checksum="C90329F4E15A31DB" modified="2005-09-27" version="1">MTTRRIVITGLGLVTSLGSDVKTVWDLLSNGVSGISKINRFDTSDLDCKIAGQVIMKSESEEYVFNAADYIPEKDLKKMDNFIHFGIAAATQAVNDSNLLDCKDLDYDRVGVIVGSGIGGLPFIEKTVICLQERGPRRISPFFIPASLINLVSGHISIKYGFTGLTDAVVTACSTGAQAIGNAARVIQSGEADVIIAGGAEGAVCRIGIAGFSAMKALSTKFNDTPELASRPWDKQRDGFVMGEGAGIVILEDYEHAKKRGAKIYGELLGYGMTSDAYHISAPHPEGKGGMKAMELALSSAQLNPESINYINAHGTSTPLGDSTEIQAIKSIFKESIYKVPISSTKSSIGHLLGAAGSVEAIFSILAMNTSITPPTLNLYESSEDPQLNLVPLNAQEHKINYCLSNSFGFGGVNISLVFGKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSQ4</accession>
    <name>TIG_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trigger factor</fullName>
            <shortName evidence="1">TF</shortName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PPIase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tig</name>
        <name type="ordered locus">Ecaj_0201</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">About half TF is bound to the ribosome near the polypeptide exit tunnel while the other half is free in the cytoplasm.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FKBP-type PPIase family. Tig subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304329.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSQ4"/>
    <dbReference type="SMR" id="Q3YSQ4"/>
    <dbReference type="STRING" id="269484.Ecaj_0201"/>
    <dbReference type="EnsemblBacteria" id="AAZ68251">
        <property type="protein sequence ID" value="AAZ68251"/>
        <property type="gene ID" value="Ecaj_0201"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0201"/>
    <dbReference type="eggNOG" id="COG0544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033058_2_2_5"/>
    <dbReference type="OMA" id="KGIKTQF"/>
    <dbReference type="OrthoDB" id="932993at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00303">
        <property type="entry name" value="Trigger_factor_Tig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005215">
        <property type="entry name" value="Trig_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008880">
        <property type="entry name" value="Trigger_fac_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037041">
        <property type="entry name" value="Trigger_fac_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008881">
        <property type="entry name" value="Trigger_fac_ribosome-bd_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036611">
        <property type="entry name" value="Trigger_fac_ribosome-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30560">
        <property type="entry name" value="PTHR30560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05698">
        <property type="entry name" value="Trigger_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05697">
        <property type="entry name" value="Trigger_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003095">
        <property type="entry name" value="Trigger_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102735">
        <property type="entry name" value="SSF102735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00115">
        <property type="entry name" value="tig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0697">Rotamase</keyword>
    <feature type="chain" id="PRO_0000256556" description="Trigger factor">
        <location>
            <begin position="1"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="1">
        <location>
            <begin position="176"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00303"/>
        </source>
    </evidence>
    <sequence length="440" mass="50401" checksum="1597C80655A7720F" modified="2005-09-27" version="1">MLDCYVVKEVSNDKLKWEYEFVIDKKYFLDQLDSKLSEIAKNVKVPGFRVGKASIDLVKKEYLNDVMTDVVRKTIESTSSDFVKSNKFGEIISSNIDIVSYPNYYSDNDSKEENLVYKLSFEVMPEAPLMDIDSIVLNGIEVDIQESDVSEFIENLKKQRPNFIVVNGAEYAVQEGDKVVIDYQNKVKGKILRGGSAKDFALVIGKGVALKEFENQLLGMRVGETKSFPLTFPDDYSVAYLAGKTTDMSVVVKSIYVVKDVQDNESVARSYGFKDVAEMENFVRKQIGQQFDQMVLTIMKKELFDYMDNTYSIDVPECVVKQEITKINKEILDSGEDIQIDVEKEAVRRVKLGMLLIRMSRHNNITIKNEDVLSFIKNNYTDYGVDINNVLKMLQSNKNFANYISGKVLEDKVINYIIKLVKKDRKVMTTKEINLMFENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSR6</accession>
    <name>Q3YSR6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="4">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ecaj_0188</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EPSP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0188"/>
    <dbReference type="EnsemblBacteria" id="AAZ68239">
        <property type="protein sequence ID" value="AAZ68239"/>
        <property type="gene ID" value="Ecaj_0188"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0188"/>
    <dbReference type="eggNOG" id="COG0128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_640516_0_0_5"/>
    <dbReference type="OMA" id="YEDHRMA"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="10">
        <location>
            <begin position="11"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="428" mass="47755" checksum="ED0C924EC2BBE8CD" modified="2005-09-27" version="1">MVLISEKIYHMCGHVDILKDNLMSHATLVLASQVIGVTKIYDLVFNDDIMLTIKALDLLGVKIKYNKSSKVCTVEGMGVGGFLCPKDALYLNNPAIYMMIGCLASCPFTSFLYGNSNISISKVMKPLLLMGARFIANDNKFPAALVGYIDMLPIRYVAKDDETKMAMIFAALNTFGTTTIISTTGKDSIVPILQYLNVGIECFINKNYVINISGQRELYSKDMCIPNDFFCMLSFIAVALVLKESEITILNVLLTDRIKSFCKVLIEMGGNLFFVNHRNNAIGEKVADLLIKGSVLQGMKCLHNNDFDIDEYLFIVIIAAYADGITVLSNVLDHILMDKRLKTVINELVKAGIRIDIEGNRLIIYGCSDNILDWNLVDACYDFKMTILFLVIGMISKNVIKIKNIRKTRDLLTIIELFNKHGAGIKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSR8</accession>
    <name>Q3YSR8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EndoU_bacteria domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0186</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304315.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68237">
        <property type="protein sequence ID" value="AAZ68237"/>
        <property type="gene ID" value="Ecaj_0186"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0186"/>
    <dbReference type="eggNOG" id="ENOG502ZA3K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089587_0_0_5"/>
    <dbReference type="OMA" id="ETECECE"/>
    <dbReference type="OrthoDB" id="1355433at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037227">
        <property type="entry name" value="EndoU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029501">
        <property type="entry name" value="EndoU_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14436">
        <property type="entry name" value="EndoU_bacteria"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142877">
        <property type="entry name" value="SSF142877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004231032" description="EndoU_bacteria domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="EndoU_bacteria" evidence="3">
        <location>
            <begin position="140"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="285" mass="32977" checksum="A07EDCBBDAF176B7" modified="2005-09-27" version="1">MNFCKYILLFIIFLPFSLVACSDVYPPLEVLKNQDDVKNSYKCLRNQSIMNPFFNMDKDSDNDCDLPVVPELNKFDRAVLNVCGNWINTPRADLFKVMLDNTEYKEYVEKLYEDLNHEVFTPNADLAQFKDELTKLWFDQHAFAHIMCGQPDILKLGGMHFFGRYIQAQENHWAGRHYDSAVVDEVSDKVYTIGVIFRNPNNYLVIDPRKGCDFLHADEIILNATKAYKAFSKHRELGGDVLKKCLYKNDGVDYTFVAKDNAIFTFYSTLTPQCDPGETECECEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YST3</accession>
    <name>Q3YST3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">8-amino-7-oxononanoate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304300.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0171"/>
    <dbReference type="EnsemblBacteria" id="AAZ68222">
        <property type="protein sequence ID" value="AAZ68222"/>
        <property type="gene ID" value="Ecaj_0171"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0171"/>
    <dbReference type="eggNOG" id="COG0156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_11_2_5"/>
    <dbReference type="OMA" id="YPYFRPI"/>
    <dbReference type="OrthoDB" id="479874at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="40"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="367" mass="40730" checksum="4C5972457E0E0DEE" modified="2005-09-27" version="1">MLDGILNQELINLKKNNLYRELSTITRSESGCFVAQNDQNLISFSCNDYLGLVGHPLLKEAAINAVNSYGVGAGASRMITGNNVIYQCLEKKLAKIYNTEMALVFSSGYLANVGVIAALTHRHDMIISDKLVHSSIIDGIKLSGAKNYRFEHNDYKDCENILKEFRKLHKHCLIIVEQVYSMNGDIAPIDHLKKLAEKYEAWLITDCAHSFGLMHCTNSDVYVGTLSKAAGVLGGYVCASEVVIKYIQNKARTFIYTTALPPMVVAAANAALDIIGGSVTDIPIKLAKFFCKNLNLAEPESHIVLLIMESVNKVLHAQQVLKQEGFLVIAIRPPTVQTPRLRFVFSVNHNLSDIEKLCYVIKQKKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSU1</accession>
    <name>Q3YSU1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein translocase subunit secE/sec61 gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0163</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304292.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0163"/>
    <dbReference type="EnsemblBacteria" id="AAZ68214">
        <property type="protein sequence ID" value="AAZ68214"/>
        <property type="gene ID" value="Ecaj_0163"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0163"/>
    <dbReference type="eggNOG" id="COG0690">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113663_8_0_5"/>
    <dbReference type="OMA" id="MSILFCS"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005807">
        <property type="entry name" value="SecE_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038379">
        <property type="entry name" value="SecE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00964">
        <property type="entry name" value="secE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="65" mass="7418" checksum="8F5F69BF81C0CF49" modified="2005-09-27" version="1">MSNVTKFLLGVKQEALQVSWASKNEVVGFLFVVILIITFMSILFCSVDFLFLKFIKIILGVIYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSU6</accession>
    <name>Q3YSU6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304287.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0158"/>
    <dbReference type="EnsemblBacteria" id="AAZ68209">
        <property type="protein sequence ID" value="AAZ68209"/>
        <property type="gene ID" value="Ecaj_0158"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0158"/>
    <dbReference type="eggNOG" id="COG1409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_635751_0_0_5"/>
    <dbReference type="OMA" id="MSIFTNH"/>
    <dbReference type="OrthoDB" id="550557at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004231033" evidence="1">
        <location>
            <begin position="25"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="106"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="431" mass="48584" checksum="2CBD336EDC7B2BCF" modified="2005-09-27" version="1">MKVMLIKIKCFFVLLILLSISADAKPLFTWSQVTSNDKLSVRVVVPEGENCPIVNVDGKKLEMTVRAFPQKGVFDDKVCELLVPQTSEEIFVDGMEVPVINKEIRKIAIIADTGCIVSFWRGKLYQQRCTSSEEWPLKKILSNIAQHYPDLIVHVGDYLYRAGKCVDVEKCGNVRGGDNSEAWKADWLGPSVLIADKAPFLFVRGNHENCDRSYMGWFRYLDPHQYPSSSDKCNNFTDSWMFNASRLNSGNLDFYVFDSSFGDEKNVLDSDIQNLKEQFLPLLKSKSSNIWFLTHRPLWSYSTHIKGGDIYYGNVSQKKAFGDLFPDNVSVVLSGHMHLSQVLDLESTNKEKYVPMQIIAGNGGALLHGVPEDKLLFKNVRIGDVIANTITNELDFGFAIVTMQEPGLTGDVIVTLYNASGKEVRQFHVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSV9</accession>
    <name>Q3YSV9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0145</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell inner membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304274.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0145"/>
    <dbReference type="EnsemblBacteria" id="AAZ68196">
        <property type="protein sequence ID" value="AAZ68196"/>
        <property type="gene ID" value="Ecaj_0145"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0145"/>
    <dbReference type="eggNOG" id="COG0573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_1_0_5"/>
    <dbReference type="OMA" id="VIRMSVL"/>
    <dbReference type="OrthoDB" id="1325025at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="144"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="374" mass="41242" checksum="A5448F860C24C09B" modified="2005-09-27" version="1">MILTTILLSITLLIGNYHKIKLNFEDKFNIITCLNITYPFFVCYSFYTEHINTVSIIITFIVLVLVLIAIYIDKEFILLKCIMFSSVGLLSLVVICIVLCLTYESALFFSKIPLTDFLFGTNWQPDPEIIDGKPVGSFGILPLLSGTLLIVIVAITIAVPLGLFSAIYISEYASKKIRYITNTILEILAGIPTVVYGYFAVVFLSPSIRSLAKYYHLNTQSENALVAGLVIGIMILPFITSMIENAIHSVPKNLRYGSMALGASRSDTIWNIIIPYALPNIISSIILSISRVIGETMIVLMAAGITAHLSFNPLHAVTTITVQIATILTGDQNFNSIQTSSAYALGLTLFIITWLLNLIALCITKKATRSIKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSY1</accession>
    <name>Q3YSY1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hypothetical membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304252.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0123"/>
    <dbReference type="EnsemblBacteria" id="AAZ68174">
        <property type="protein sequence ID" value="AAZ68174"/>
        <property type="gene ID" value="Ecaj_0123"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0123"/>
    <dbReference type="eggNOG" id="COG1463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107027_1_1_5"/>
    <dbReference type="OMA" id="QYQFPKD"/>
    <dbReference type="OrthoDB" id="807474at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030970">
        <property type="entry name" value="ABC_MlaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003399">
        <property type="entry name" value="Mce/MlaD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02470">
        <property type="entry name" value="MlaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04430">
        <property type="entry name" value="OM_asym_MlaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="MlaD" evidence="2">
        <location>
            <begin position="40"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="149" mass="16239" checksum="EE4192ACA9F8D929" modified="2005-09-27" version="1">MHRSNVIEIFAGFIVLIAALSIGIIAFKKLPFKNTSHSCYIVKAHFPSVDGLDMGDDITLSGVKIGTVTSISLDRNFTPIVTMCIQKNVLLPSDSSASLSLSNFLGRRYIDIALGSNQDSIPAGGFIEYTNSDLNLNVMLTRLVNRFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSZ9</accession>
    <name>Q3YSZ9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purE</name>
        <name evidence="7" type="ordered locus">Ecaj_0105</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304234.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0105"/>
    <dbReference type="EnsemblBacteria" id="AAZ68156">
        <property type="protein sequence ID" value="AAZ68156"/>
        <property type="gene ID" value="Ecaj_0105"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0105"/>
    <dbReference type="eggNOG" id="COG0041">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094982_2_2_5"/>
    <dbReference type="OMA" id="SNSIDGW"/>
    <dbReference type="OrthoDB" id="1701464at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="6">
        <location>
            <begin position="8"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="167" mass="17634" checksum="AEE7605105E3BB29" modified="2005-09-27" version="1">MSINQNTVDVAVIMGSQSDFPTMNNSISILKELNITHKVMIISAHRTPDRLYTFAKSAEDNGFKVIIAGAGGAAHLPGLVASLTHIPVIGVPIKSSNLNGIDSLLSIVQMPKGIPVATMSIGSSGAYNAAIIAASILSLENDIIKDNLKTWRKNLSDSVKLIPDTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT25</accession>
    <name>Q3YT25_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304208.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0079"/>
    <dbReference type="EnsemblBacteria" id="AAZ68130">
        <property type="protein sequence ID" value="AAZ68130"/>
        <property type="gene ID" value="Ecaj_0079"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0079"/>
    <dbReference type="HOGENOM" id="CLU_1084769_0_0_5"/>
    <dbReference type="OMA" id="WIQQLED"/>
    <dbReference type="OrthoDB" id="959243at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="262" mass="30450" checksum="1EDE2666646FFB8F" modified="2005-09-27" version="1">MVTREIWGWFFMVLITTSCSVCAVLSYLDIKKGLVEFNQQFNQQYKRAMENDVRSILVQVNQSLLNSYLSKQKENCPVQRIVTSENNDVISCSSEEWLAKLLLSVINLREAFLGDISVKSIIYSIKPMLLRLDDSLINDAVAEIERLDSKKLTTFNRLKFSFKKIARELHYSRSNMVQKVFFKWIVVKNQNDVLLHNLKEVESYLDDNLWSDALTFAQKELSSVVELELWIQQLEDIVSMEKSISIIYDQLSQYIIKLPGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT51</accession>
    <name>Q3YT51_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Trans-hexaprenyltrans transferase</fullName>
            <ecNumber evidence="3">2.5.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0053</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304182.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0053"/>
    <dbReference type="EnsemblBacteria" id="AAZ68104">
        <property type="protein sequence ID" value="AAZ68104"/>
        <property type="gene ID" value="Ecaj_0053"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0053"/>
    <dbReference type="eggNOG" id="COG0142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_0_5"/>
    <dbReference type="OMA" id="NIVAVMS"/>
    <dbReference type="OrthoDB" id="1861068at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="325" mass="36450" checksum="C645ACD7D2C3A9F8" modified="2005-09-27" version="1">MTNEFNNTLLQLKSLIQQDLDAMEILISQHSDKHIALITEIIHHLIKSGGKRIRPIIFFIICKMLNCTNENKILIAAAIEFIHNATLLHDDVIDESDLRRGKKTSNFIWGNKASILVGDFLLAISFQWLIECKNLNILSILSQTSNTIVVGEIQQMLSSHNINISKEKYIEIISAKTASLFGATCESAAHLAQVNLQESKALKSFGNNFGIAFQIMDDILDYTASNAQFGKKLGNDLSTGKTTLPVIIAYQNATPEEKKFWDQISVNQNLEQAISYIKSHNAIEKSLEIATAYIDQAKACLTIFSESNYKMCLNKLLDCILYRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT64</accession>
    <name>Q3YT64_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ABC-type transport system involved in resistance to organic solvents auxiliary component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304169.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0040"/>
    <dbReference type="EnsemblBacteria" id="AAZ68091">
        <property type="protein sequence ID" value="AAZ68091"/>
        <property type="gene ID" value="Ecaj_0040"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0040"/>
    <dbReference type="eggNOG" id="COG2854">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1313789_0_0_5"/>
    <dbReference type="OMA" id="EEFTNID"/>
    <dbReference type="OrthoDB" id="1716096at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008869">
        <property type="entry name" value="MlaC/ttg2D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042245">
        <property type="entry name" value="Tgt2/MlaC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05494">
        <property type="entry name" value="MlaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230945" evidence="1">
        <location>
            <begin position="24"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="209" mass="24161" checksum="7D4C14D78AEBA9F9" modified="2005-09-27" version="1">MISNFICKLFFISLFFLFNNAFGYDSTEACNEYIRPCYFIVTLKNQIEIITQEIKDKAELYAGIQSIVDRVLDIREISKFIAGSYWNTMSTDEQEKFVNEYGQYVKRMYSKQLCKYSTYDMSILSVKNPKEGHYLINTRLSSENDNHNFIIVEFKLISAGDGFLLSDLRVNNAISLSVTQRSMIKNIVSKQGIDGMLSYFQSENSAAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT67</accession>
    <name>Q3YT67_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Heme exporter protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ecaj_0037</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the CcmB/CycW/HelB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304166.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0037"/>
    <dbReference type="EnsemblBacteria" id="AAZ68088">
        <property type="protein sequence ID" value="AAZ68088"/>
        <property type="gene ID" value="Ecaj_0037"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0037"/>
    <dbReference type="eggNOG" id="COG2386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1254316_0_0_5"/>
    <dbReference type="OMA" id="WICATFV"/>
    <dbReference type="OrthoDB" id="1965979at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003544">
        <property type="entry name" value="Cyt_c_biogenesis_CcmB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026031">
        <property type="entry name" value="Cyt_c_CcmB_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03379">
        <property type="entry name" value="CcmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002764">
        <property type="entry name" value="CcmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="11 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="191"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR002764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAZ68088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="220" mass="24495" checksum="A23D5B0EA099E940" modified="2005-09-27" version="1">MCGSTVFLHELKLLMSRKCNLLNAIILFVLIISTALFTMQNTEIVKALPTILWVCSISTIHISMCNLFENDYANGSLEQMLIQDRIPEIVIFFKIFSHWIYVGIPVSIISMFIDYVILGSSIYATLVLGLSLSISLLVISFISAVGDTLVLGRGYGVIIAQILTLPIMIPVLIYFSLLFESLRNGLYEENLMLLGLLIASLIPLSIIFVSFSIKLAVEHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT68</accession>
    <name>Q3YT68_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DnaK suppressor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0036</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304165.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0036"/>
    <dbReference type="EnsemblBacteria" id="AAZ68087">
        <property type="protein sequence ID" value="AAZ68087"/>
        <property type="gene ID" value="Ecaj_0036"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0036"/>
    <dbReference type="eggNOG" id="COG1734">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043144_2_2_5"/>
    <dbReference type="OMA" id="KKGEEYM"/>
    <dbReference type="OrthoDB" id="1932026at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037187">
        <property type="entry name" value="DnaK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000962">
        <property type="entry name" value="Znf_DskA_TraR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01258">
        <property type="entry name" value="zf-dskA_traR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109635">
        <property type="entry name" value="SSF109635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51128">
        <property type="entry name" value="ZF_DKSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DksA C4-type" evidence="6">
        <location>
            <begin position="58"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="dksA C4-type" evidence="4">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="138"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="152" mass="18035" checksum="2F43F0AED33DD54D" modified="2005-09-27" version="1">MLKKDYNTAFPEDEDDSNYMNHKQLEYFRQKLIKWRETLQKESEELATELLRPQVDADLTDMATREHNIVLILKNSKRNEALIVEINKAIQRIDDGLYGYCEETGEKIGIARLEANPITLYSIEEQERREKQQKLYSNDQESDFNNNFLNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT77</accession>
    <name>Q3YT77_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304156.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0027"/>
    <dbReference type="EnsemblBacteria" id="AAZ68078">
        <property type="protein sequence ID" value="AAZ68078"/>
        <property type="gene ID" value="Ecaj_0027"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0027"/>
    <dbReference type="HOGENOM" id="CLU_150535_0_0_5"/>
    <dbReference type="OMA" id="VIKNCEY"/>
    <dbReference type="OrthoDB" id="1767282at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="143" mass="16563" checksum="F8888D52BE605BF8" modified="2005-09-27" version="1">MSDVPFVNNRNETKSHKITWEIVKNQKYKQTHLLQISCLYIITIHNKNHNISLPTDNVISNILLRINTTMESVLLNKLLSIEILKGISSHKFISKKKNNIARLQDISQFFNSHFNIKLPRSIEESFIAEHKEAVQLLKSSISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT85</accession>
    <name>Q3YT85_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Type IV secretion system protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0019</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Part of the Type IV secretion system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304148.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0019"/>
    <dbReference type="EnsemblBacteria" id="AAZ68070">
        <property type="protein sequence ID" value="AAZ68070"/>
        <property type="gene ID" value="Ecaj_0019"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0019"/>
    <dbReference type="eggNOG" id="COG0630">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_3_1_5"/>
    <dbReference type="OMA" id="EAWVEKK"/>
    <dbReference type="OrthoDB" id="473670at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043684">
        <property type="term" value="C:type IV secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044097">
        <property type="term" value="P:secretion by the type IV secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014155">
        <property type="entry name" value="VirB11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02788">
        <property type="entry name" value="VirB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="3">
        <location>
            <begin position="17"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="332" mass="36585" checksum="292B601B754D4941" modified="2005-09-27" version="1">MTTSYAALDTYLEPLQSIFNEDGVNEVSINQACEVWVENRGNIRCESIPILNLSHLKALGRLIAQATEQKISEENPLLSATLPNGYRIQIVFPPACEATAVAMSIRKPSAMQLSLDDYEKMGAFDQAITETKENSIDNELNELLKQKKIKQFLECAVINKKNIIISGGTSTGKTTFTNAALRSIPKDERLITVEDAREIVLSNHPNRVHLISSKGGQGRAKVTTQDLIEACLRLRPDRIIVGELRGAEAFSFLRAINTGHPGSISTLHADTPMMALEQLKLMVMQAGLGIPPDQIINYITNIVDIVIQLKRASGGVRYVSEILFTKSLQKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT98</accession>
    <name>Q3YT98_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nicotinate-nucleotide diphosphorylase (carboxylating)</fullName>
            <ecNumber evidence="4">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0006</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304135.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0006"/>
    <dbReference type="EnsemblBacteria" id="AAZ68057">
        <property type="protein sequence ID" value="AAZ68057"/>
        <property type="gene ID" value="Ecaj_0006"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0006"/>
    <dbReference type="eggNOG" id="COG0157">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039622_0_1_5"/>
    <dbReference type="OMA" id="DMIMLKD"/>
    <dbReference type="OrthoDB" id="1718114at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="PTHR32179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="QRPTase_N" evidence="11">
        <location>
            <begin position="20"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="QRPTase_C" evidence="10">
        <location>
            <begin position="108"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="275" mass="30289" checksum="EB24AD8F80BCD896" modified="2005-09-27" version="1">MKVSFSEIINNALKEDLGENGDITTNSILLDEKINFSINAREDLVVCGIPILEEIFNTKKEYIKYKVHKNDGNIAGKNSTLVSGEALAIHLMPIERVILNFIQHASGIASITRQFVNEVSGTKAKIRSTRKTTPGLRMLDKYSVCVGGGENYRNSLYDGVLIKDNHIASCGSITLAIQRLRMNLKNAYVAIECDNISQIEESLSNNVDMILLDNMSIGEIKQAVEIVNGKSVLEVSGCVNIRNVRSIALTGVDYISIGCITNSFKNKDIGLDIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YTA0</accession>
    <name>Q3YTA0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="10">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glycyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="10">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">glyS</name>
        <name evidence="12" type="ordered locus">Ecaj_0004</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304133.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0004"/>
    <dbReference type="EnsemblBacteria" id="AAZ68055">
        <property type="protein sequence ID" value="AAZ68055"/>
        <property type="gene ID" value="Ecaj_0004"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0004"/>
    <dbReference type="eggNOG" id="COG0751">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007220_2_1_5"/>
    <dbReference type="OMA" id="LPIPKRM"/>
    <dbReference type="OrthoDB" id="213210at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00255">
        <property type="entry name" value="Gly_tRNA_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015944">
        <property type="entry name" value="Gly-tRNA-synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30075">
        <property type="entry name" value="PTHR30075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02092">
        <property type="entry name" value="tRNA_synt_2f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01045">
        <property type="entry name" value="TRNASYNTHGB"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00211">
        <property type="entry name" value="glyS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DALR_1" evidence="11">
        <location>
            <begin position="585"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="701" mass="80896" checksum="C61510F470C77757" modified="2005-09-27" version="1">MPLELLFECLSEEIPPEMQNSAYLYINSYIKKELNRYNINFKSIEVFVTPNRLTLYVNNIVNSSSNNLLKRKGPKVSADKIAIEGFLKKVNKKISDLIICKVGNDEFYYAEISNNIEINTENIISSIIENMLTNFPWDKKMRWGTGNTHWIRPIINIMCILDGKVLPIKFADIEANNKSRGNRFTKFFEVKDFNSYISQLKKNNVILCQEERMNSILQQIQNITEENNLVCENNQNLINKLNGILEYPIVIMGSVDEKFHDLPKELILSVMHNHQKYLAAFTGGKITNFITISTVSNDAIISGHNNVLNARLSDASFLINKDKEYNIDYYIEKLKNIVFHAKLGSVFEKVNRIVALSKYISMWIPHSSLIKVERASQLSKFDLSTLAVKEFPELQGIIGGYYASYFNEELEISESIVNHYEPTSSKKECPSSPITIAVSISDKTDSLVGMIVAGEQVTGSRDPFSIRRMAISVIRTIIENNLNIPIKLLVEKSVSLYSYAITEKNAINKIKDVISRKNRRKDIIFYVLEFFSNRFKIMLKEDGIKEDIINAVIESRKFNNLLIIKKEANLLNEYVFTNNGIEIIKAYKRLSNISDIEDNISITTKQKPNKKLFSANEEHDLYKEISYQKNNIKKLIKENKFKESLDNLYNFSCCVNRFMDNVKVNDKMNKELYKNRISLVKSAFYIFDLVTDFSKIKNTHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQP6</accession>
    <name>Q3YQP6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome segregation ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0928</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305032.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0928"/>
    <dbReference type="EnsemblBacteria" id="AAZ68959">
        <property type="protein sequence ID" value="AAZ68959"/>
        <property type="gene ID" value="Ecaj_0928"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0928"/>
    <dbReference type="eggNOG" id="COG1192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_1_4_5"/>
    <dbReference type="OMA" id="VQCEYFA"/>
    <dbReference type="OrthoDB" id="729012at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="3"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="255" mass="28406" checksum="04C04526F122C54F" modified="2005-09-27" version="1">MSKVFAIVNQKGGVGKTTTSINLSTAFSIVNKKTLLIDLDPQGNSSTGFGITYEQRTNTVYEVLINNLPISSAVVTTEIPNLHLLPSTVDLSAAEVELTQVQQREFILKKSLSEVKNSYDYIFIDCPPSLGLLTVNALIAADSIMIPLQCEFFALEGLSHLIKTIEIVKKHLNPLLSIEGIILTMYDKRNKLSEQVEEDIRKYLKESVYKTVIPRNVRLSEAPSHGKPAIIYDFKCAGSQAYIYLAKEILKKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQQ4</accession>
    <name>Q3YQQ4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68951">
        <property type="protein sequence ID" value="AAZ68951"/>
        <property type="gene ID" value="Ecaj_0920"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0920"/>
    <dbReference type="HOGENOM" id="CLU_127010_0_0_5"/>
    <dbReference type="OMA" id="GVGTLGH"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="182" mass="19750" checksum="71B4319122EAEF52" modified="2005-09-27" version="1">MDIFSNELNATVHVNGTTYEGKVIIDNNGNFDTNLSLADGVDTLGHLCGNISQNNETKENSYILEYIFEQRIVYPTLPILHSFNGQIVSSAEEALPHQIAFDNSNDNIKIILSDSEIVQPVTNAKESQAEVSKPVTDVKENQDGAPQPAANTPQEKQESVPTPADGVNNDPTKEGASQPNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQT6</accession>
    <name>Q3YQT6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent dethiobiotin synthetase BioD</fullName>
            <ecNumber evidence="5">6.3.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DTB synthetase</fullName>
            <shortName evidence="5">DTBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Dethiobiotin synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bioD</name>
        <name evidence="6" type="ordered locus">Ecaj_0888</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)-dethiobiotin + ADP + 3 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the dethiobiotin synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0888"/>
    <dbReference type="EnsemblBacteria" id="AAZ68919">
        <property type="protein sequence ID" value="AAZ68919"/>
        <property type="gene ID" value="Ecaj_0888"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0888"/>
    <dbReference type="eggNOG" id="COG0132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072551_3_1_5"/>
    <dbReference type="OMA" id="YPHAPNI"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00161"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00336">
        <property type="entry name" value="BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004472">
        <property type="entry name" value="DTB_synth_BioD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210">
        <property type="entry name" value="PTHR43210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006755">
        <property type="entry name" value="DTB_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00347">
        <property type="entry name" value="bioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="15"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="180"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="231" mass="26040" checksum="6D21729D11AB8297" modified="2005-09-27" version="1">MYEMSAYFITSSGTDVGKTFVTTALCWHFIKRGKIVQAIKPVISGWNDTDILNNDTYKILCSLNIECNSSNIARISPWRLCYPHAPNIAARLENIELNYSEILEFCYQCISQSYDNLFIEGVGGVMSPITDDRTCLDLICDLNVKVILVIGSYLGSISHTLTALRVLSGMSVKVVLTIKNEGDTNVDDIVKFIYEYTGKSVYVQHYVAGSFDLWEKTSDDIVNFLNLYDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQW8</accession>
    <name>Q3YQW8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Methionine aminopeptidase</fullName>
            <shortName evidence="2">MAP</shortName>
            <shortName evidence="2">MetAP</shortName>
            <ecNumber evidence="2 3">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">map</name>
        <name evidence="5" type="ordered locus">Ecaj_0856</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2 3">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="2 3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304964.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0856"/>
    <dbReference type="MEROPS" id="M24.001"/>
    <dbReference type="EnsemblBacteria" id="AAZ68887">
        <property type="protein sequence ID" value="AAZ68887"/>
        <property type="gene ID" value="Ecaj_0856"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0856"/>
    <dbReference type="eggNOG" id="COG0024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_0_0_5"/>
    <dbReference type="OMA" id="GDHAYTF"/>
    <dbReference type="OrthoDB" id="1285136at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070084">
        <property type="term" value="P:protein initiator methionine removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="18"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="266" mass="29775" checksum="8FD343E83DAEECD7" modified="2005-09-27" version="1">MSNLERSITIHNKEDFQYMRNAGKLAAEVLDFITSYVKPGVSTNELNNLCHDFIINAGAIPAPLGYRGYPKSICTSKNFVVCHGIPDDIPLKNGDIVNIDVTVIVDGWHGDTSRMYWVGEPSIKAKRLCEAAYKALMVAIEQVMPGQKLNQIGLAIEKEMKKYGYSIVRDYCGHGLGRVFHASPNVVHYYDPDDTVIIREGMFFTIEPMINAGKCNTMLNKKDGWTVTTRDLSLSAQFEHSLGVTENGVEIFTLSPQNMHYPPYMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQX3</accession>
    <name>Q3YQX3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0851</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68882">
        <property type="protein sequence ID" value="AAZ68882"/>
        <property type="gene ID" value="Ecaj_0851"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0851"/>
    <dbReference type="HOGENOM" id="CLU_1203286_0_0_5"/>
    <dbReference type="OMA" id="RYESAQR"/>
    <dbReference type="OrthoDB" id="2152510at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="225" mass="23994" checksum="3EFCD9762C4CCA29" modified="2005-09-27" version="1">MVLFMKAHSTSIRNFQPLERAAIIIAVLGLAAFLFAAAACSDRFQRLQLTNPFVIAGMVGLAVLLVASLTAALSICLTKSKQVTQHAIRHRFGYESSTSSSVLLAISIISLLLAAAFCGKIMGNDNPDLFFSKMQELSNPLVVAAIVAVSVFLLSFVMYAAKNIISPDKQTHVIILSNQQTIEEAKVDQGMNILSAVLPAAGIDIMTIASCDILAVSSRGSSQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQY0</accession>
    <name>Q3YQY0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HflC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0844</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">HflC and HflK could regulate a protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the band 7/mec-2 family. HflC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304952.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0844"/>
    <dbReference type="MEROPS" id="I87.001"/>
    <dbReference type="EnsemblBacteria" id="AAZ68875">
        <property type="protein sequence ID" value="AAZ68875"/>
        <property type="gene ID" value="Ecaj_0844"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0844"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059167_1_0_5"/>
    <dbReference type="OMA" id="DFFAFYR"/>
    <dbReference type="OrthoDB" id="1714999at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052547">
        <property type="term" value="P:regulation of peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03405">
        <property type="entry name" value="SPFH_HflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010200">
        <property type="entry name" value="HflC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42911">
        <property type="entry name" value="PTHR42911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005651">
        <property type="entry name" value="HflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="4">
        <location>
            <begin position="23"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR005651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="290" mass="33335" checksum="F3157D68AF49CFA1" modified="2005-09-27" version="1">MSSNPFKFILGFLTLVIVVISLNSIFIVDEAHQSIVLQFGRVVKQIHNSGLYFKLPFIQKVVYVDKRIIDISSDSREVIAADQKRFIVDSYAKYRIVDPVKFYQTVRTEIGLKNRLSSIIESNIREKIGNVSLINFLNEARSEVMTIIQEGVSKESEKFGIEMIDVRIKRADLPEENSTAIFRRMQTDREKEAKEIRAEGEEASQRIKSDADLQTRIIIADAIKEAQIIRGNGEAKASKIYNDVLKVDPNFFSFYRTMQAYRHAFNGKNTRIILSPNNDFINLFNKERGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR56</accession>
    <name>Q3YR56_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0768</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0768"/>
    <dbReference type="EnsemblBacteria" id="AAZ68799">
        <property type="protein sequence ID" value="AAZ68799"/>
        <property type="gene ID" value="Ecaj_0768"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0768"/>
    <dbReference type="HOGENOM" id="CLU_3199202_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="45" mass="5348" checksum="B2FBE1D8F4816170" modified="2005-09-27" version="1">MVKDLEAERRVRAMRERADRYNENQLRADVVLEQRNLQGYLGIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR68</accession>
    <name>Q3YR68_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304864.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68787">
        <property type="protein sequence ID" value="AAZ68787"/>
        <property type="gene ID" value="Ecaj_0755"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0755"/>
    <dbReference type="HOGENOM" id="CLU_1097254_0_0_5"/>
    <dbReference type="OMA" id="DINHCIS"/>
    <dbReference type="OrthoDB" id="1589119at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="242" mass="28018" checksum="2E11DD1D1349FF07" modified="2005-09-27" version="1">MMDEIYLAIVSVLTLIIFILMIYSILKKCRDSKKDFDVNNDSGLPVSALNISYSRIRNSVICDLKKQLKVKMKELYYSGTEFIEAKSPQHGVLMVKIGFRYVQDQIFNFIDKINNYDVYKQMLLLTRFISETEDSVLMCNSHKEILLKCDLQNMSCIRALLKNVFNLVKNIGDEEQIKLMSEYYIKLDYGLAFIVKGMSFTIKNVDILNIFCRDTRPRGNGIFYDDISFVAISLGDIENCVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR83</accession>
    <name>Q3YR83_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304849.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0740"/>
    <dbReference type="EnsemblBacteria" id="AAZ68772">
        <property type="protein sequence ID" value="AAZ68772"/>
        <property type="gene ID" value="Ecaj_0740"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0740"/>
    <dbReference type="HOGENOM" id="CLU_1400588_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="194" mass="21751" checksum="50F878427D180B38" modified="2005-09-27" version="1">MQNGIVIALSVLVVLLSILLLIALTKCYCVLLDNKKLAAQNDEIVRINRGITADNKSLRNQSSKLLSIVKDRLLRDKEKCKELAEMTKQQIDEKLSEFVPLIIKQAVGVLRDNIAPDIKVDKSVIAGAIDYCVQSMQEGFLGCVDNMQVAREHDIKLLEEQVEEIWNNTVEEEVPWQVSDLAVNGMYSVNDKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR87</accession>
    <name>Q3YR87_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0736</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304845.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68768">
        <property type="protein sequence ID" value="AAZ68768"/>
        <property type="gene ID" value="Ecaj_0736"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0736"/>
    <dbReference type="HOGENOM" id="CLU_1439021_0_0_5"/>
    <dbReference type="OrthoDB" id="2175042at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="188" mass="21308" checksum="8A81A8C305E7A8B4" modified="2005-09-27" version="1">MDTTAIVLLVLLIVMLLLLVLLAVAAVKCQKQYNFLKEENKKLLACCEEASDYSQELVSQNDKLKKESERIKSKSDKLERELKNKMQKFFSDQVSEYEDLKESCIELINIRGESVKKLFDDVKQDMSPESVKCLIDTNILFLQKSITYEIDSSKNTLEKRLSDQFPSNDIEGASVSSTGGNVQPNDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR99</accession>
    <name>Q3YR99_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304833.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68756">
        <property type="protein sequence ID" value="AAZ68756"/>
        <property type="gene ID" value="Ecaj_0724"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0724"/>
    <dbReference type="HOGENOM" id="CLU_1872171_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="136" mass="14825" checksum="FF0B9ACB679B4603" modified="2005-09-27" version="1">MTNTEIGLTVTLILLLSVILLALLLAFCVFRYCGSYLDRKVESMQRLLEEPRIMDQHKLGNAGGMEEVVQCRLGNADGVGDPLLQASGGSSITVEEMRIRRLGRFSTNKEGNINPRSGLSSSKIVTLDSLVQDKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRA2</accession>
    <name>Q3YRA2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304830.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68753">
        <property type="protein sequence ID" value="AAZ68753"/>
        <property type="gene ID" value="Ecaj_0721"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0721"/>
    <dbReference type="HOGENOM" id="CLU_1238618_0_0_5"/>
    <dbReference type="OrthoDB" id="2140838at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="223" mass="25330" checksum="84E7FE38FC0726DF" modified="2005-09-27" version="1">MKIEYILAIVFAVVVAILLIISLLYWLLKSSRRLKEQAGGQEIFKSIGSGRIPIVPENKNVVNALFKVNASLAEHVSCQFSVMQILLDAIKYMMLENGEEEALILKLNEICIDIFSAYYVIDFRSRVSTIRINEYNLLANDLVRSYQDMCDICEDLLSLIEQHKVTDYKQCCAQLMKNIVLECKNECLMLMLQEQSYNCSVLPSIGLEDSFVNGVGSCQQDMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRC0</accession>
    <name>Q3YRC0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3'-5' exonuclease</fullName>
            <ecNumber evidence="3">3.1.26.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0703</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.26.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304812.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0703"/>
    <dbReference type="EnsemblBacteria" id="AAZ68735">
        <property type="protein sequence ID" value="AAZ68735"/>
        <property type="gene ID" value="Ecaj_0703"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0703"/>
    <dbReference type="eggNOG" id="COG0349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042387_0_0_5"/>
    <dbReference type="OMA" id="FMRVDTF"/>
    <dbReference type="OrthoDB" id="1462689at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="SSF47819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="2">
        <location>
            <begin position="3"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="386" mass="45009" checksum="7B3474B9AEC24026" modified="2005-09-27" version="1">MLIDSVKNLEHMCNNLLMLQPKFIAIDTEFIRNSSEYYPRLCLMQVAYGREQFVIDVLASDMDLSPLNSILYNKNIVKVFHDCRQDIDALLTKFPKIPNPIFDTQVAAMFCYCYENAVGYSRLVEQFLGISLDKLSLKRSNWMLRPLSPDKIQYALNDVIYLHELYQILYDNLVDSGKLLWFLEEMDNIVLQELNYSNVCDIPDLSSDITREEIIVVKSVIEWREKLAQFFNLNRELILRNKVMLSLIKDFIRKSDISTLSKYIKKEYLTHGIVADLLEIINNNQDVNVDELYNIDTNYNQSILNVLLILLCNLCYKNNISQKLVTSKGSLIKLLNGKFSYVMKGWRYEFFGCEIEKFMQGKSKIIVSAQVLDNNFIDIMTTLTDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRC2</accession>
    <name>Q3YRC2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0701</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304810.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0701"/>
    <dbReference type="EnsemblBacteria" id="AAZ68733">
        <property type="protein sequence ID" value="AAZ68733"/>
        <property type="gene ID" value="Ecaj_0701"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0701"/>
    <dbReference type="eggNOG" id="COG0278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_2_1_5"/>
    <dbReference type="OMA" id="KGTKLMP"/>
    <dbReference type="OrthoDB" id="2001991at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097573">
        <property type="term" value="F:glutathione oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014434">
        <property type="entry name" value="Monothiol_GRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="PTHR10293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005894">
        <property type="entry name" value="Monothiol_GRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00365">
        <property type="entry name" value="TIGR00365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="8">
        <location>
            <begin position="18"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="110" mass="12647" checksum="1C10D70AAEED033E" modified="2005-09-27" version="1">MKNDIMDKIKHDIENNDVVLYMKGDADFPQCGFSSVVVSILKKMKVNFKSINVLEDLELREAIKEFTNWPTIPQLYVKGEFIGGCDIVKEMYHSGELQELFSKNNLISTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRD7</accession>
    <name>Q3YRD7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavin-dependent thymidylate synthase</fullName>
            <shortName evidence="1">FDTS</shortName>
            <ecNumber evidence="1">2.1.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FAD-dependent thymidylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thymidylate synthase ThyX</fullName>
            <shortName evidence="1">TS</shortName>
            <shortName evidence="1">TSase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thyX</name>
        <name evidence="2" type="ordered locus">Ecaj_0686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP + H(+) + NADPH = (6S)-5,6,7,8-tetrahydrofolate + dTMP + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:29043"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.148"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate synthase ThyX family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.148" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0686"/>
    <dbReference type="EnsemblBacteria" id="AAZ68718">
        <property type="protein sequence ID" value="AAZ68718"/>
        <property type="gene ID" value="Ecaj_0686"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0686"/>
    <dbReference type="eggNOG" id="COG1351">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067790_0_0_5"/>
    <dbReference type="OMA" id="EWARLMP"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050797">
        <property type="term" value="F:thymidylate synthase (FAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01408">
        <property type="entry name" value="ThyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003669">
        <property type="entry name" value="Thymidylate_synthase_ThyX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036098">
        <property type="entry name" value="Thymidylate_synthase_ThyX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934">
        <property type="entry name" value="PTHR34934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02511">
        <property type="entry name" value="Thy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69796">
        <property type="entry name" value="SSF69796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02170">
        <property type="entry name" value="thyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51331">
        <property type="entry name" value="THYX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1 2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Involved in ionization of N3 of dUMP, leading to its activation" evidence="1">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="102"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="105"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="113"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="201"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="293" mass="34352" checksum="8441F121357878F5" modified="2005-09-27" version="1">MGGVMMELMESKLTKRVVVPELEEILYKEHKVLDKGFVRLVDYMGSDESVVQSARISYGRGTKSVNQDAALINYLMRHSHTTPFEMCEIKFHIKLPIFVARQWVRHRTANVNEYSARYSVLDREFYIPEHDQIAKQSENNAQGRGSSLSHEDAEYVTDLLVHDSNMVYETYNKFLEKGISREISRVNLTLNYYTEWYWKIDLHNLLHFLKLRSEMNAQYEIRVYAKAILEIVKKWVPLTYAAFVEYSLESQHLSKSALDVVRRLIAGEEVAREDTSIGKREWKELMSILENEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRD9</accession>
    <name>RUVB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Holliday junction ATP-dependent DNA helicase RuvB</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvB</name>
        <name type="ordered locus">Ecaj_0684</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with RuvA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304793.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRD9"/>
    <dbReference type="SMR" id="Q3YRD9"/>
    <dbReference type="STRING" id="269484.Ecaj_0684"/>
    <dbReference type="EnsemblBacteria" id="AAZ68716">
        <property type="protein sequence ID" value="AAZ68716"/>
        <property type="gene ID" value="Ecaj_0684"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0684"/>
    <dbReference type="eggNOG" id="COG2255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055599_1_0_5"/>
    <dbReference type="OMA" id="IHRMSRP"/>
    <dbReference type="OrthoDB" id="874197at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00016">
        <property type="entry name" value="DNA_helic_RuvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041445">
        <property type="entry name" value="AAA_lid_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004605">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008824">
        <property type="entry name" value="RuvB-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008823">
        <property type="entry name" value="RuvB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42848">
        <property type="entry name" value="PTHR42848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17864">
        <property type="entry name" value="AAA_lid_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05491">
        <property type="entry name" value="RuvB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05496">
        <property type="entry name" value="RuvB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00635">
        <property type="entry name" value="ruvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000235366" description="Holliday junction ATP-dependent DNA helicase RuvB">
        <location>
            <begin position="1"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="58"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00016"/>
        </source>
    </evidence>
    <sequence length="329" mass="36998" checksum="CA70E08AF46B688A" modified="2005-09-27" version="1">MKNILQSTECLEDQQNVSIRPNLLDEFIGQSSVVNNLKIFINAAYTRKEPMDHVLLYGPPGLGKTTLAHIIAKELKVNFRSTAGPLLSKAGDLAAILTNLQAKDILFIDEIHRLNRNIEEILYSAMEDFCLDIIVGEGCGARTLRVDLPKFTLVGATTRIGLLSNPLRDRFGIPIHLEFYSTEELIKVIQRAAKVIKTDISDSGAQEISLRARGTPRIALRLLRRIRDFIEVTEHNKTITDTFADKALLRLGIDKLGLDRQDIQYLKFIHDSNNPTGIDTISSGLSEDTGNIEETIEPYLIKINFIQRTPRGRVITQKAISYLKEQSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRE5</accession>
    <name>Q3YRE5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ecaj_0678</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in electron transfer from bc1 complex to aa3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304787.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0678"/>
    <dbReference type="EnsemblBacteria" id="AAZ68710">
        <property type="protein sequence ID" value="AAZ68710"/>
        <property type="gene ID" value="Ecaj_0678"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0678"/>
    <dbReference type="eggNOG" id="COG3474">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060944_4_0_5"/>
    <dbReference type="OMA" id="KGYIPGT"/>
    <dbReference type="OrthoDB" id="1939800at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="PTHR11961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00604">
        <property type="entry name" value="CYTCHRMECIAB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0349">Heme</keyword>
    <keyword evidence="9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="12">
        <location>
            <begin position="72"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="173" mass="19377" checksum="CF7AEFFEF2D7A380" modified="2005-09-27" version="1">MDGFHLNKIFTAILFSSFIILFISNVVDLLYNPNPHAEQVRGYQINIEEQPVNTAINSTETQTDIETLIAQADITKGKEITKKCISCHTFEKGGANRVGPNLWNIIGNKKAHLGDKFNYSKAMLNKGGTWEHQDLFEFLTKPQAYIKGTRMAFAGISKPQDVADLIAYLKSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRF9</accession>
    <name>Q3YRF9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HPTransfase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304773.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0664"/>
    <dbReference type="EnsemblBacteria" id="AAZ68696">
        <property type="protein sequence ID" value="AAZ68696"/>
        <property type="gene ID" value="Ecaj_0664"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0664"/>
    <dbReference type="eggNOG" id="COG5385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1308517_0_0_5"/>
    <dbReference type="OMA" id="DAANDVM"/>
    <dbReference type="OrthoDB" id="1464071at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018762">
        <property type="entry name" value="ChpT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10090">
        <property type="entry name" value="HPTransfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPTransfase" evidence="1">
        <location>
            <begin position="79"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="210" mass="23871" checksum="0CE99DF4488A1143" modified="2005-09-27" version="1">MQNSDILSNVELVSARLLHDLAGSVGAIVNYIECLSDDEGLCEDMMSLLSDAANDVMNKFKLLKQAYSISDDNGDFGVTKHNIKNYLKRKKILLEWDVDVQLFDVELVEKVNKLLTNMVMILMYFMINGSKVKVTLSKSSDGGLLLEIVACAQKIEVHDSIKSILDRDISNRELNTKNIQVNFLMMLLDNYHAKMTYEVQETIFKFFVCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRG4</accession>
    <name>Q3YRG4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
            <ecNumber evidence="3">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ecaj_0659</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the transfer of pyrophosphate from adenosine triphosphate (ATP) to 6-hydroxymethyl-7,8-dihydropterin, an enzymatic step in folate biosynthesis pathway.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HPPK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0659"/>
    <dbReference type="EnsemblBacteria" id="AAZ68691">
        <property type="protein sequence ID" value="AAZ68691"/>
        <property type="gene ID" value="Ecaj_0659"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0659"/>
    <dbReference type="eggNOG" id="COG0801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097916_1_2_5"/>
    <dbReference type="OMA" id="QVILRQN"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="SSF55083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPPK" evidence="13">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAZ68691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="172" mass="19566" checksum="23CB218C962B4B14" modified="2005-09-27" version="1">MCGPLSNDIVILALGSNCGNMLGYIKSAVSMLPLYNMNYSYLYKTPALLPENAADHWDTPFLNMVVSGYTNLSPHSMLEQVKSIEKKLGRISHERWAPRPIDIDIILWENTVLDLDNLSIPHKQMHCRDFVLVPLCDIYARLPHPTSKTSIQDMLLKLKDINLIKQNANLFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRH3</accession>
    <name>CH10_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Co-chaperonin GroES</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">10 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Chaperonin-10</fullName>
            <shortName evidence="1">Cpn10</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">groES</name>
        <name evidence="1" type="synonym">groS</name>
        <name type="ordered locus">Ecaj_0648</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heptamer of 7 subunits arranged in a ring. Interacts with the chaperonin GroEL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GroES chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304759.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRH3"/>
    <dbReference type="SMR" id="Q3YRH3"/>
    <dbReference type="STRING" id="269484.Ecaj_0648"/>
    <dbReference type="EnsemblBacteria" id="AAZ68682">
        <property type="protein sequence ID" value="AAZ68682"/>
        <property type="gene ID" value="Ecaj_0648"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0648"/>
    <dbReference type="eggNOG" id="COG0234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132825_1_0_5"/>
    <dbReference type="OMA" id="KVFYRQW"/>
    <dbReference type="OrthoDB" id="1965002at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="PTHR10772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000025255" description="Co-chaperonin GroES">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00580"/>
        </source>
    </evidence>
    <sequence length="94" mass="10390" checksum="4F3EAD64F2FF58C5" modified="2005-09-27" version="1">MNLSMLHDNVLIEALEEGNSNSPIQLPDSAKKKPTKGKVVAVGPGVYNSNGNILPMNIKVGEVVFYRQWAGNEIEFNDKKYIVMKESDIIAKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRH8</accession>
    <name>Q3YRH8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Intradiol ring-cleavage dioxygenase</fullName>
            <ecNumber evidence="6">1.13.11.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0643</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.3" evidence="6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304754.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0643"/>
    <dbReference type="EnsemblBacteria" id="AAZ68677">
        <property type="protein sequence ID" value="AAZ68677"/>
        <property type="gene ID" value="Ecaj_0643"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0643"/>
    <dbReference type="eggNOG" id="COG3485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089557_0_0_5"/>
    <dbReference type="OMA" id="LSDKNMC"/>
    <dbReference type="OrthoDB" id="1456634at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018578">
        <property type="term" value="F:protocatechuate 3,4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03459">
        <property type="entry name" value="3_4-PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039387">
        <property type="entry name" value="3_4-PCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="SSF49482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230939" evidence="4">
        <location>
            <begin position="24"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="INTRADIOL_DIOXYGENAS" evidence="5">
        <location>
            <begin position="62"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="224" mass="25372" checksum="81B811A655F76007" modified="2005-09-27" version="1">MNYFVKLLLCLLMSTAYTYNALAVDPVLVNCKKTPEIYDISNKPSKFSLSNNLRRKTGTPLTADGELIYIVGRVTDENCIPIANANVLIWHTNAHGIYQDNQGPSGKSELILEELDMYDYNFNGSGKSVTDNLGNYSFITIVPGTNSQNKIPRVHFILQHSEFPEFNTTMFFPEYNNSTDNQFKNIDPNLNDRLVAQYTKNALGIKTYHFNITIAGKTPYKSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRJ3</accession>
    <name>Q3YRJ3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0628"/>
    <dbReference type="EnsemblBacteria" id="AAZ68662">
        <property type="protein sequence ID" value="AAZ68662"/>
        <property type="gene ID" value="Ecaj_0628"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0628"/>
    <dbReference type="HOGENOM" id="CLU_2069404_0_0_5"/>
    <dbReference type="OMA" id="FPQNDNS"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="118" mass="13378" checksum="AA583CFB7B855694" modified="2005-09-27" version="1">MSFINATIGFADDLNSDGNVYDAETDSNENTGEGLYLDNDQDYDENDDFYYDEDDVYEDEDDIYNDNLMDNEENDVQDDTEEDNDDAPEVNGEKGNKLNAKSKIGEGKSDDKKKLLKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRK9</accession>
    <name>RL3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplC</name>
        <name type="ordered locus">Ecaj_0612</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L19.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Methylated by PrmB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304724.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRK9"/>
    <dbReference type="SMR" id="Q3YRK9"/>
    <dbReference type="STRING" id="269484.Ecaj_0612"/>
    <dbReference type="EnsemblBacteria" id="AAZ68646">
        <property type="protein sequence ID" value="AAZ68646"/>
        <property type="gene ID" value="Ecaj_0612"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0612"/>
    <dbReference type="eggNOG" id="COG0087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044142_2_0_5"/>
    <dbReference type="OMA" id="KRMAGRY"/>
    <dbReference type="OrthoDB" id="1270636at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_B">
        <property type="entry name" value="Ribosomal_L3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_L3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_L3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="PTHR11229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000241343" description="50S ribosomal protein L3">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="1">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <sequence length="231" mass="25245" checksum="06D6ECC9F58863FC" modified="2005-09-27" version="1">MAKRIGLFLEKVGHTAIFDNEGKRIPVTLLHLRDSFIISTKTKDINGYNAVVLGVESSVNIKKPQLKILEKSNITAKCRIFESRVDNLDGIVKGAKISITHFVENQYIDVTGYSLGKGFAGVMKRHNFKGLKASHGVSIAHRSQGSTGQCQDPGRVYKGKKMAGHLGSSKVTVQNLKVILVDQERSLLVVKGNNIPGAKGSYVFVKDAVKKSVPKNSFPVCTEDLKLDNIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRL2</accession>
    <name>RL2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplB</name>
        <name type="ordered locus">Ecaj_0609</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304721.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRL2"/>
    <dbReference type="SMR" id="Q3YRL2"/>
    <dbReference type="STRING" id="269484.Ecaj_0609"/>
    <dbReference type="EnsemblBacteria" id="AAZ68643">
        <property type="protein sequence ID" value="AAZ68643"/>
        <property type="gene ID" value="Ecaj_0609"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0609"/>
    <dbReference type="eggNOG" id="COG0090">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_2_1_5"/>
    <dbReference type="OMA" id="SCIELRP"/>
    <dbReference type="OrthoDB" id="961486at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_B">
        <property type="entry name" value="Ribosomal_L2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_L2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_L2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000237182" description="50S ribosomal protein L2">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="224"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="276" mass="30003" checksum="B344C71D812159F3" modified="2005-09-27" version="1">MGIKNLNSVTASLRGTVLLDKSMLWKGKPEKSLVSYKISCGGRNSRGVITVRHRGRGHKRLYRIIDFKRNKVGVSATVERLEYDPNRTAFIALLSYDDGEKSYIIAPNGLKKGDVVISGEGSDILPGNCLMLKSIPVGTFVHNVELRPGNGGVIARSAGTYAQLMSKDGVYVLLRLSSGEIRKVLSDCRATIGIVSNLDNQNVKLGKAGRNRWLGIRPTVRGVAMNPIDHPHGGGEGKTSGGRNPVTPWGVPTKGKKTRKRNKSSNKYIKRVSDKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRL8</accession>
    <name>RS17_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsQ</name>
        <name type="ordered locus">Ecaj_0603</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304715.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRL8"/>
    <dbReference type="SMR" id="Q3YRL8"/>
    <dbReference type="STRING" id="269484.Ecaj_0603"/>
    <dbReference type="EnsemblBacteria" id="AAZ68637">
        <property type="protein sequence ID" value="AAZ68637"/>
        <property type="gene ID" value="Ecaj_0603"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0603"/>
    <dbReference type="eggNOG" id="COG0186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_1_1_5"/>
    <dbReference type="OMA" id="HPMYGKF"/>
    <dbReference type="OrthoDB" id="2035880at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_B">
        <property type="entry name" value="Ribosomal_S17_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019984">
        <property type="entry name" value="Ribosomal_S17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_S17/S11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="PTHR10744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03635">
        <property type="entry name" value="uS17_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000233475" description="30S ribosomal protein S17">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <sequence length="83" mass="9730" checksum="E1562F2D47E1458D" modified="2005-09-27" version="1">MKEKKRKGMSNKKVLTGVVVATKCDKTIKVMVSRMVRHKTYKKIVKKRKNYVVHDEYNKCKCGDVVKIQEHIPISATKRWIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRM8</accession>
    <name>Q3YRM8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secY</name>
        <name evidence="11" type="ordered locus">Ecaj_0593</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0593"/>
    <dbReference type="EnsemblBacteria" id="AAZ68627">
        <property type="protein sequence ID" value="AAZ68627"/>
        <property type="gene ID" value="Ecaj_0593"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0593"/>
    <dbReference type="eggNOG" id="COG0201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030313_0_2_5"/>
    <dbReference type="OMA" id="FAMWLGE"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="PTHR10906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="SSF103491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="436" mass="48880" checksum="E0B3AFD43321E25F" modified="2005-09-27" version="1">MSQKMDLLSWNKDNLLGKLWFTLLCLIVYRLGTYIPIPGINPDIIDAVVSKHSVGVLGIFNVFSGGALGRMTVFALNVMPYIVSSIIIQLLSVTVPRLNELRQNGELGRMKINNYIRYMTIFFCIVQGSVILLGLEKMNSENSIVVIDPGLLFRIVGISSLLGGTMFLLWLGERINKNGIGNGVSMIIFTGIVAELPGSFSSMFLLGKNGNVPIFVILLMVLIFFALLLLIIFVEKSYRKILVQYPKRQVKNKLYGSSSTYIPLKINISGVIPPIFANALLLSPITVANFHQGSPWSDFILMYFSSGKLLYIVCYALLIIFFAFFYTAFVFDAKETSEILKKNGGFVPGKRPGNATCEYFNYVIKRLTVLGSIYLTVICIIPELIRYNYSVSFTLGGTSFLIIVNVIIDTFSQVQTHLYSSRYNTLIRKSELWKIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRN0</accession>
    <name>RS13_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsM</name>
        <name type="ordered locus">Ecaj_0591</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304703.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRN0"/>
    <dbReference type="SMR" id="Q3YRN0"/>
    <dbReference type="STRING" id="269484.Ecaj_0591"/>
    <dbReference type="EnsemblBacteria" id="AAZ68625">
        <property type="protein sequence ID" value="AAZ68625"/>
        <property type="gene ID" value="Ecaj_0591"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0591"/>
    <dbReference type="eggNOG" id="COG0099">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_1_2_5"/>
    <dbReference type="OMA" id="YRGLRHK"/>
    <dbReference type="OrthoDB" id="1772647at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019980">
        <property type="entry name" value="Ribosomal_S13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="PTHR10871:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03631">
        <property type="entry name" value="uS13_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000230503" description="30S ribosomal protein S13">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="123" mass="13999" checksum="108F04A881E4124B" modified="2005-09-27" version="1">MARIAGVNIPTNKRVVIALTYIYGIGLSLANRICEGCNIDHNVRVVNLSDDEIIRIRNFIRENYIVEGDLRKEVSMNIKFLTDIGCYRGLRHRRGLPVRGQRTHTNAKTRKGRSKLPVAAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRR1</accession>
    <name>Q3YRR1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Multisubunit sodium/proton antiporter, MrpG subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0559"/>
    <dbReference type="EnsemblBacteria" id="AAZ68594">
        <property type="protein sequence ID" value="AAZ68594"/>
        <property type="gene ID" value="Ecaj_0559"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0559"/>
    <dbReference type="eggNOG" id="COG1320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121334_2_2_5"/>
    <dbReference type="OMA" id="ATKCDTG"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005451">
        <property type="term" value="F:monovalent cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098662">
        <property type="term" value="P:inorganic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005133">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703">
        <property type="entry name" value="PTHR34703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03334">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01300">
        <property type="entry name" value="CPA3_mnhG_phaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="93" mass="10275" checksum="B0BF51A3AB42520F" modified="2005-09-27" version="1">MIILVLGMFLVITSVIGVIRFPDFYTKLHPAGITDSLGATLILIGLAMQFELSIVTIKILLLICFLWITCTTASYALASSAYHKDKKRENNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRR2</accession>
    <name>Q3YRR2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF4040 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0558</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304671.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0558"/>
    <dbReference type="EnsemblBacteria" id="AAZ68593">
        <property type="protein sequence ID" value="AAZ68593"/>
        <property type="gene ID" value="Ecaj_0558"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0558"/>
    <dbReference type="eggNOG" id="COG2111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102546_0_0_5"/>
    <dbReference type="OMA" id="YDTFGET"/>
    <dbReference type="OrthoDB" id="1792987at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MbhD-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="DUF4040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4040" evidence="8">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="181" mass="19845" checksum="273A5B3EB3A11A1F" modified="2005-09-27" version="1">MLSDVNFIMNIVLSLFLVFVSFSLVFFRNLTLNVVVMCIFSFLMALLYLIMDAPDVAITEASVGGGISTVLMLMALSLIGKNEGKVHFNIKIFPVIIALGMFISLMYVIPDLPVFGDPNSFAKNHVASYYLKNTYNNIGIPNVVTAILASFRGYDTFGETVVIFTASLGISLLLAKDNNHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRT5</accession>
    <name>Q3YRT5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type IV secretory pathway, VirB3-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0534</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0534"/>
    <dbReference type="EnsemblBacteria" id="AAZ68570">
        <property type="protein sequence ID" value="AAZ68570"/>
        <property type="gene ID" value="Ecaj_0534"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0534"/>
    <dbReference type="eggNOG" id="COG3702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158477_3_0_5"/>
    <dbReference type="OMA" id="FIELFMV"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007792">
        <property type="entry name" value="T4SS_VirB3/TrbD/AvhB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05101">
        <property type="entry name" value="VirB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="101" mass="11647" checksum="DA4AC64178DA4C03" modified="2005-09-27" version="1">MVFIMAGSVKADQLFKGLTRPTMLFGVSYIFAMLNFMICIMIFMYTNDFRALFILGPGIHGIGFLASSKEPLFLELFMLRMKKCSKCLNRFYHNSNSYDVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRW3</accession>
    <name>Q3YRW3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">DNA polymerase III subunit epsilon</fullName>
            <ecNumber evidence="3 9">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dnaQ</name>
        <name evidence="11" type="ordered locus">Ecaj_0506</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3 9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304620.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0506"/>
    <dbReference type="EnsemblBacteria" id="AAZ68542">
        <property type="protein sequence ID" value="AAZ68542"/>
        <property type="gene ID" value="Ecaj_0506"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0506"/>
    <dbReference type="eggNOG" id="COG0847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047806_2_1_5"/>
    <dbReference type="OMA" id="FHVYLNP"/>
    <dbReference type="OrthoDB" id="1985371at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06131">
        <property type="entry name" value="DNA_pol_III_epsilon_Ecoli_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006054">
        <property type="entry name" value="DnaQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006309">
        <property type="entry name" value="DnaQ_proteo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00573">
        <property type="entry name" value="dnaq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01406">
        <property type="entry name" value="dnaQ_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Exonuclease" evidence="10">
        <location>
            <begin position="8"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="239" mass="27526" checksum="EECA981B49976855" modified="2005-09-27" version="1">MSYYKGVREVVLDTETTGLDPDGGDRIIEIGCVELIDRVFTGKVFHEYINPERDIPYYATKIHGITIDMLKDKPKFSEIADKFLEFVNNSVLVIHNAGFDIKFIKMELDKIQKDYDSDFQVVDTLILARKKFPGVSSTLDALCRRFNISLQDRKFHGALLDATLLGKVYVQLMGGLQRSLDFSYSNNIIDTSNINNQCEDVAIEPRFYEVSEEEITQHKMLLEKIKNPIWNSVFYMKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRW4</accession>
    <name>Q3YRW4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine-monophosphate kinase</fullName>
            <shortName evidence="2">TMP kinase</shortName>
            <shortName evidence="2">Thiamine-phosphate kinase</shortName>
            <ecNumber evidence="2">2.7.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">thiL</name>
        <name evidence="5" type="ordered locus">Ecaj_0505</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + thiamine phosphate = ADP + thiamine diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15913"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thiamine-monophosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.16" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304619.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0505"/>
    <dbReference type="EnsemblBacteria" id="AAZ68541">
        <property type="protein sequence ID" value="AAZ68541"/>
        <property type="gene ID" value="Ecaj_0505"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0505"/>
    <dbReference type="eggNOG" id="COG0611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046964_3_0_5"/>
    <dbReference type="OMA" id="HFRRDWS"/>
    <dbReference type="OrthoDB" id="1016556at2"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02194">
        <property type="entry name" value="ThiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02128">
        <property type="entry name" value="TMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005303">
        <property type="entry name" value="Thiam_monoph_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01379">
        <property type="entry name" value="thiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AIRS" evidence="3">
        <location>
            <begin position="22"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="4">
        <location>
            <begin position="144"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="113"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="314" mass="35218" checksum="2428C5769E899A13" modified="2005-09-27" version="1">MKEFEYIKNYIYKLDNDSLIGDDAAIIDYTNKLLVTKDILIEGIHFFKNCNPTTLAKKALRSNLSDIAAMGSTPYGYCLGLVLPHNITQDWWQNFTNSLKEEHKKFNIKLLGGDTTSHKEDKIIISITAFGTSNGNILRRSEAKIGDFIYVSGNIGDAALGLLIYQKIINKNYNNLKKKYDIPQPRINLGISINNIASSCIDISDGLIQDLEHICHSSQVGAEIYLNKIPLSNEAQEIITNSPKYINNILSGGDDYELIFTINPQFSSLIKEISYKNKIKISKIGEITPGNSVTLYDKNRNIITLNSKGFNHFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRW7</accession>
    <name>Q3YRW7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mg chelatase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0502</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family. ComM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304616.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0502"/>
    <dbReference type="EnsemblBacteria" id="AAZ68538">
        <property type="protein sequence ID" value="AAZ68538"/>
        <property type="gene ID" value="Ecaj_0502"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0502"/>
    <dbReference type="eggNOG" id="COG0606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026145_1_1_5"/>
    <dbReference type="OMA" id="STNPCPC"/>
    <dbReference type="OrthoDB" id="581802at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032508">
        <property type="term" value="P:DNA duplex unwinding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004482">
        <property type="entry name" value="Mg_chelat-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025158">
        <property type="entry name" value="Mg_chelat-rel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="PTHR32039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039:SF7">
        <property type="entry name" value="PTHR32039:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13335">
        <property type="entry name" value="Mg_chelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00368">
        <property type="entry name" value="TIGR00368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="208"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="506" mass="55850" checksum="4DAB31A7C4FF1962" modified="2005-09-27" version="1">MIAQVKSVAFYGINTINVEVQIHIAKGIPAFNIVGLPDKAIAESRERIRAALNSINLSLPSQRITINLSPADLFKEGSHYDLPIAVGLLMTIGIIPKINDLPYIILGELALDASITSVSGVLPAAISAQNQKIGIICPYNNGEEASLIKNISILSPRHLTSLINYFNKNETIPTPSNKLLIENPEYLDMKDVKGQSIAKRALEIAAAGGHNVLMVGPPGTGKSMIAKRLPGILPDLTYKQIIEINTIKSITKKCNNSISTTRPFRDPHSSLSIAAMVGGGKNVKPGEITLAHQGILFLDELPEFSRSVLDALRQPLENKEILISRANINIKYPANFQLIAAMNPCRCGYFGDSSKACSKAPKCAIEYQNKISGPLNDRIDIHIEVPYVNMLLYDNYKTSENSKTIKERVIIAQKLQKIRYGNLFSNATISENMVTKFLIPDKSGMQLLRNVLQEKSTSAREYIKILKVARTIADLALSESISYNHIAESLSYTKQKYIKEYKFFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRX6</accession>
    <name>Q3YRX6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="3">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0492</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = 5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304607.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0492"/>
    <dbReference type="EnsemblBacteria" id="AAZ68529">
        <property type="protein sequence ID" value="AAZ68529"/>
        <property type="gene ID" value="Ecaj_0492"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0492"/>
    <dbReference type="eggNOG" id="COG0212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066245_0_1_5"/>
    <dbReference type="OMA" id="LRIFMTK"/>
    <dbReference type="OrthoDB" id="1816478at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="PTHR23407:SF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="9"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="116"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="173" mass="19757" checksum="4D5C727C6D7A4E3C" modified="2005-09-27" version="1">MVDDIKNRKIELRKKYRRLRKEVQNKNGASYALLKNYIDFIMIEKASIVSGYMPIDGEIDVLPLMNYLIEHACIVAIPIVNENSKVLLFHELNTVNIVVPNILIVPLIAFDKNLNRLGFGGGYYDNTISTLRPNCKVIGVGYDIQLCDLIPTESHDQALDIVITEKNIYENKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRY5</accession>
    <name>Q3YRY5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0483</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304598.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68520">
        <property type="protein sequence ID" value="AAZ68520"/>
        <property type="gene ID" value="Ecaj_0483"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0483"/>
    <dbReference type="HOGENOM" id="CLU_2449933_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="89" mass="10338" checksum="AC62C964744C0188" modified="2005-09-27" version="1">MDLTLLVLSDGKILLYPTDRCGIRRRADGKIRNYNLDDTDLSAQLGYPDPQSTYHNFHTMFLSRFTQSVIISSTKLLEDLVFFFVKVKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-02-09" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRZ4</accession>
    <name>NUON_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit N</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoN</name>
        <name type="ordered locus">Ecaj_0474</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304589.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRZ4"/>
    <dbReference type="SMR" id="Q3YRZ4"/>
    <dbReference type="STRING" id="269484.Ecaj_0474"/>
    <dbReference type="EnsemblBacteria" id="AAZ68511">
        <property type="protein sequence ID" value="AAZ68511"/>
        <property type="gene ID" value="Ecaj_0474"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0474"/>
    <dbReference type="eggNOG" id="COG1007">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_1_3_5"/>
    <dbReference type="OMA" id="FYIRVIV"/>
    <dbReference type="OrthoDB" id="1664448at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00445">
        <property type="entry name" value="NDH1_NuoN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010096">
        <property type="entry name" value="NADH-Q_OxRdtase_suN/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01770">
        <property type="entry name" value="NDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000391143" description="NADH-quinone oxidoreductase subunit N">
        <location>
            <begin position="1"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00445"/>
        </source>
    </evidence>
    <sequence length="474" mass="52900" checksum="737F1F1CD04206F8" modified="2005-09-27" version="1">MYWNNFVYIIPEVFLVSSSLILLLLGIVLNKKVIHVLSLISLLVTVGIVGINLEIEPQLIFNGLLKSDLYISIAKIIILLSSSSVLFMLLVSGKEYCYEFSIMILFAVFGLITLIAANSLLSFYLSFEIQSITLYALTCFDKSAIKSSESGIKYFVLSALSSCIMLYGISMLYGYTTQVGFSGIYNFFSNNENIPLGAILGVILILISIFFKLSASPFHMWVPDVYQGTSTIMTMFFSIVPKSTFIFLLMRILNEVLPSLSKDWQQIMICVSMLSIFVSAFGAMRQTNLKRLFSYAAIGHIGYMLISLAINTTVSNAATIMYLLLYVVMNIGLFCILIQYDDDDCNLLNLKGLHNKSPVIAFCIAVIMLSMAGIPPLAGFFAKYDVLSSLVENGFIKIAVTFALVSAVSCYYYLKIIKVMYFDLSDSSCDVSISNKKLCFVLLLTVVVNLVFFVFVGHVRNIISYFLHFNFFNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRZ6</accession>
    <name>Q3YRZ6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304587.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0472"/>
    <dbReference type="EnsemblBacteria" id="AAZ68509">
        <property type="protein sequence ID" value="AAZ68509"/>
        <property type="gene ID" value="Ecaj_0472"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0472"/>
    <dbReference type="HOGENOM" id="CLU_418417_0_0_5"/>
    <dbReference type="OMA" id="KVHVKED"/>
    <dbReference type="OrthoDB" id="953009at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="641" mass="74720" checksum="17E932B0D772775E" modified="2005-09-27" version="1">MFRRNILNILLILIFSFVVSCSNKIKYQFSRKYSPIYNPEGEAFGNDEEFYRAYSIYKERRDSATSDIGKNSKHVDTRKKLRRKVQVKNVDPLEEYDVFLEKEDHDLMIDRNGVNLVDVEGAKFIVPIENDMPDTMRDKKHVGVGVSGPNVDDSKLPIVKVEDKSKLQDTKDKKRDDSKLPVIKVEDKSKLQDTKDKKRDDSKLPVIKVEDKSKLQDTKDKKRDDSKLPVIKVEDKSKLQDTKDKKRDDSKLPVIKVEDKSKLQDTKDKKRDDSKLPVVKVEDKSKLQDTKDKKRDDSKLPVVKVEDKSKLQDTKDKKRDDSKLPVIKVEDKSKLQDTKDKKRDDSKLPVIKVEDKSKLQDTKDKKRDDSKLPVIKVEDKSKLQDTKDKKRDDSKLPVIKVEDKSKLQDTKDKKRDDSKLPVVKVEDKSKLQDTEDKKRDDSKLPIVEKVEDKSKLQDSSDYSNDNQSDKKEHGLLSLLKFPKIEDDKHEDSNDELEDDKVNNDDGILLSQPKDSVESSQHIPKEDEQKNNRKPLHFLSFLKEPSDEQHDAKKGVQKVENKNDQDKSPILPVKEEENVKIIEDNEQGNVIKDGDNNHQVVLSQEEEELLQNIKKIKEYDEDYTITYYYDDDDYDDTEYYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS08</accession>
    <name>Q3YS08_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase epsilon chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP synthase F1 sector epsilon subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">F-ATPase epsilon subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">atpC</name>
        <name evidence="11" type="ordered locus">Ecaj_0460</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304575.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0460"/>
    <dbReference type="EnsemblBacteria" id="AAZ68497">
        <property type="protein sequence ID" value="AAZ68497"/>
        <property type="gene ID" value="Ecaj_0460"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0460"/>
    <dbReference type="eggNOG" id="COG0355">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1956129_0_0_5"/>
    <dbReference type="OMA" id="HENFIME"/>
    <dbReference type="OrthoDB" id="1696893at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="SSF51344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0139">CF(1)</keyword>
    <keyword evidence="9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP-synt_DE_N" evidence="10">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="128" mass="14741" checksum="572DDB8257F962A1" modified="2005-09-27" version="1">MKYLSVELITPDDFSEITNVLSVTSYTTDGEFVILPGHEKFMIELRSGIIKIRTDGDISIFYVLNPVLKVDLYNCKVIANQFINTCNVDAVILKKYKEDIEKILHRIDDKILLKMAKKQLTFIDNIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS13</accession>
    <name>Q3YS13_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative 3-hydroxyacyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="1">1.1.1.35</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.35" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0455"/>
    <dbReference type="EnsemblBacteria" id="AAZ68492">
        <property type="protein sequence ID" value="AAZ68492"/>
        <property type="gene ID" value="Ecaj_0455"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0455"/>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_686495_0_0_5"/>
    <dbReference type="OMA" id="WSNTLIY"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="407" mass="46802" checksum="5EFA9C2F475BE591" modified="2005-09-27" version="1">MQIQKVAIVNFKRIDISSIINYIVKCNISVVVIILNKADNILQYQEDSILVSTVHLNDDLSCLRDVRWVININSEKEEEDVILIRNIYSKILSHISHNVIISASSSVYLDIIREMQCNVSCNLLIASFFNSDNTIRLIECANYYSSQVKCDNTIQILSNSFSNLIVCNNIPGLVLDRVIVFWLMISLIGAYNFNINVEEVDFIISNECMGIPTGVFNLLDKMGLDNFILKVKYLIKHLPDDYLCKLYNFTPTVVLQMISDGYTGSTGKIGGFYRFYELYGGNNNQVIDLYTGLYRQAYVVSRDFEKIQDLFCANNKHCQFMWYVWSNTLIYVASLIPNLSHNISVIDKAIKLAYGWKHGPFEMIDLINDFTNNKFFDLHDNLPQILSIKKNMYNSKKQCLNIDGNYI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS23</accession>
    <name>RF1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide chain release factor 1</fullName>
            <shortName evidence="1">RF-1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prfA</name>
        <name type="ordered locus">Ecaj_0442</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Methylated by PrmC. Methylation increases the termination efficiency of RF1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304560.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS23"/>
    <dbReference type="SMR" id="Q3YS23"/>
    <dbReference type="STRING" id="269484.Ecaj_0442"/>
    <dbReference type="EnsemblBacteria" id="AAZ68482">
        <property type="protein sequence ID" value="AAZ68482"/>
        <property type="gene ID" value="Ecaj_0442"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0442"/>
    <dbReference type="eggNOG" id="COG0216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_0_1_5"/>
    <dbReference type="OMA" id="ISDHRVG"/>
    <dbReference type="OrthoDB" id="928964at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00093">
        <property type="entry name" value="Rel_fac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004373">
        <property type="entry name" value="RF-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00019">
        <property type="entry name" value="prfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000263269" description="Peptide chain release factor 1">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="1">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="359" mass="40884" checksum="B9ED2F6DD5F5EB4C" modified="2005-09-27" version="1">MSFDSNLEELYQKFCKLKSILEDPSQLSVDSFVAASKEYSELLPVISVIDQYNSLQKDIADLEELINNPETDHELKSLAKEEFYERQKQLPKIKNKLKLSLIPKDRDDARNAILEIRAGTGGEEAALFVTDLYRMYTKYAEQKNWKFEQINSSSTGIGGHKEVSLCISGSNVFARLKFESGVHRVQRVPETEASGRLHTSAATVAVLPEIEEVDLKIDEKDLRIDVYRSSGPGGQSVNTTDSAVRITHIPSGIVVIQQDEKSQHKNKNKALKVLRARLYNLEKQKRDSEISQMRKSQIGSGDRSERIRTYNFPQSRITDHRINLTLYRLEDIMKEGNLDEFIDALIAEDEANKLKNLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS25</accession>
    <name>Q3YS25_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304558.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68480">
        <property type="protein sequence ID" value="AAZ68480"/>
        <property type="gene ID" value="Ecaj_0438"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0438"/>
    <dbReference type="HOGENOM" id="CLU_118038_0_0_5"/>
    <dbReference type="OrthoDB" id="2117201at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="200" mass="23425" checksum="6E7104672435D344" modified="2005-09-27" version="1">MQLLLLVDNNNRAFLSDMNLYNYKREKFIPRNKYIKLKNTKGKEIQLLNHIDNSDLYGTIFTRFTIISSTKFTNETVILRGTNRYSHDVFLALLLDKHKFRNFLDKEDELCLFVSDLNEICIFLNENNYLGSGLTNERSDKQLFINLKNNMTKLKPKSDKEKFCTRFSFFNEENIMVNSIIVPAELSNISTTNLATVILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS35</accession>
    <name>Q3YS35_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304548.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68470">
        <property type="protein sequence ID" value="AAZ68470"/>
        <property type="gene ID" value="Ecaj_0428"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0428"/>
    <dbReference type="HOGENOM" id="CLU_083566_0_0_5"/>
    <dbReference type="OMA" id="NTREFPA"/>
    <dbReference type="OrthoDB" id="1882792at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021387">
        <property type="entry name" value="DUF3023"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11224">
        <property type="entry name" value="DUF3023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="290" mass="33326" checksum="4F8C4E08EA918616" modified="2005-09-27" version="1">MQQSIYISKIVNDYLCIKGDNFSFIYSECYCIGNTPENNDTLEIHVLKTYNSNKKITPNGNSVFSLNTRYPLKTIMQLNMQLKKINLINLSQNDLKEESISAKLYIFVKRNKLQQFIEDVLNNDKPGAVVVPSEYGSIIYTMLYTDDFVKYFDEETALTNFSRIASAPKNYPEFRIIHKQHSIISKLLNNMKCISQNNLISDKTSTISSTKNSNTIIRNPHEYKSDDVVTTVSTKTSPSIITEQQESDDHSTISKLKTLKKSRKRQRYGRLLLVKPSLSTIQEEPSVMIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS37</accession>
    <name>NUOD_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit D</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoD</name>
        <name type="ordered locus">Ecaj_0426</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304546.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS37"/>
    <dbReference type="SMR" id="Q3YS37"/>
    <dbReference type="STRING" id="269484.Ecaj_0426"/>
    <dbReference type="EnsemblBacteria" id="AAZ68468">
        <property type="protein sequence ID" value="AAZ68468"/>
        <property type="gene ID" value="Ecaj_0426"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0426"/>
    <dbReference type="eggNOG" id="COG0649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_1_5"/>
    <dbReference type="OMA" id="IMGTSME"/>
    <dbReference type="OrthoDB" id="473681at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01358">
        <property type="entry name" value="NDH1_NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="PTHR11993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01962">
        <property type="entry name" value="NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000357809" description="NADH-quinone oxidoreductase subunit D">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01358"/>
        </source>
    </evidence>
    <sequence length="393" mass="44780" checksum="AC0ADA7059319A0B" modified="2005-09-27" version="1">MSDQVKITPMTLNFGPQHPAAHGVMRLVLEMGGEVIERIDPHIGLLHRGTEKLIEYKTYLQALPYFDRLDYVSPMAQEHAYSLCVEKLLKCEIPIRAKYLRVIFCELTRILNHLLNISSQALDIGAMTPLLWMFEEREKILGFYERASGARFHSAYIRPGGVAADVPSDLIDDIFKFVSTFSKFMDDIDDLLTENRIWKQRNVDIGVVSKKQALDWGFSGPMLRACGIPWDLRKSQPYEIYEDLEFEIPVGENGDCYDRYLVRMAEIRQSIKLLEQCLDRLPDGPVKTDDRKIAPPKRSEMKESMEALIHHFKLYSEGYSVPVGETYMAVEAPKGEFGVYIVSDGTNKPYRCRIRAPGFAHLQAIDIIAKGHMLADLTAIIGSLDIVFGEIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS59</accession>
    <name>Q3YS59_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome c-type biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0403</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Possible subunit of a heme lyase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CcmH/CycL/Ccl2/NrfF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304524.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0403"/>
    <dbReference type="EnsemblBacteria" id="AAZ68446">
        <property type="protein sequence ID" value="AAZ68446"/>
        <property type="gene ID" value="Ecaj_0403"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0403"/>
    <dbReference type="eggNOG" id="COG3088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107187_4_1_5"/>
    <dbReference type="OMA" id="PFKNRSD"/>
    <dbReference type="OrthoDB" id="1984854at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005616">
        <property type="entry name" value="CcmH/CycL/Ccl2/NrfF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038297">
        <property type="entry name" value="CcmH/CycL/NrfF/Ccl2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03918">
        <property type="entry name" value="CcmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="132" mass="15513" checksum="EFE5B1089698CDA5" modified="2005-09-27" version="1">MQIKNNLVFIITFTLSFSSAYAFSIDKKLVNNDMELRAINLFKIVRCLICSGESIYESQSQLAYDMRAMIRNKIQNGYNDDQIITELRNYYGNQIIINPPYNSYTYLLWILPALIFIIGIILSVKFMYNRRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS64</accession>
    <name>MDH_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Malate dehydrogenase</fullName>
            <ecNumber evidence="1">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mdh</name>
        <name type="ordered locus">Ecaj_0398</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of malate to oxaloacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 3 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304519.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS64"/>
    <dbReference type="SMR" id="Q3YS64"/>
    <dbReference type="STRING" id="269484.Ecaj_0398"/>
    <dbReference type="EnsemblBacteria" id="AAZ68441">
        <property type="protein sequence ID" value="AAZ68441"/>
        <property type="gene ID" value="Ecaj_0398"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0398"/>
    <dbReference type="eggNOG" id="COG0039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045401_2_1_5"/>
    <dbReference type="OMA" id="MGWTSQA"/>
    <dbReference type="OrthoDB" id="870724at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01339">
        <property type="entry name" value="LDH-like_MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00487">
        <property type="entry name" value="Malate_dehydrog_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011275">
        <property type="entry name" value="Malate_DH_type3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01763">
        <property type="entry name" value="MalateDH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_0000241947" description="Malate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00487"/>
        </source>
    </evidence>
    <sequence length="313" mass="33762" checksum="8CF3E7B4C1836F23" modified="2005-09-27" version="1">MIKRKKIALIGAGNIGGMIAYLIRLKNLGDVVLLDVNDGIAKGKALDMAESSPVGKYNGEILGTNNYADIEGADAIVVTAGITRKPGMSREDLINTNVNIIKEVADNIGKYAPNAFVVVVTNPLDVMVFAMHKYSKLSSNMVVGMAGILDSARFSYFIAKELNVSVDNVNSLVLGGHGDLMLPLVKYSSVAGISIADLIKIDLITQDKVDAIIERTRKGGEEIVSLLKIGSAYYAPAESALLMIDSYLNDRRLILPCSVYLKGEYGVSNLFVGVPVIIGRNGVEKIIELELTEQEKNIFDNSVKLIKNLVSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS65</accession>
    <name>Q3YS65_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydroneopterin aldolase</fullName>
            <ecNumber evidence="2">4.1.2.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.2.25" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0397"/>
    <dbReference type="EnsemblBacteria" id="AAZ68440">
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    <dbReference type="OrthoDB" id="1858654at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006760">
        <property type="term" value="P:folic acid-containing compound metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FolB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02152">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00526">
        <property type="entry name" value="folB_dom"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FolB" evidence="1">
        <location>
            <begin position="3"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="118" mass="13725" checksum="7C1CC507DC05C4BA" modified="2005-09-27" version="1">MKIQIHDLAVYTCIGLRRWEKVIRQKIIVDCDVTLYCYNAISDIEDTIDYSLFTDSLINFANSNSFELLEIMASELLDYIMKDKRICHCYLKLYKPTAINIDGKVSVELESMQNDVNT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS78</accession>
    <name>RL36_EHRCJ</name>
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        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmJ</name>
        <name type="ordered locus">Ecaj_0384</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
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        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304505.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS78"/>
    <dbReference type="SMR" id="Q3YS78"/>
    <dbReference type="STRING" id="269484.Ecaj_0384"/>
    <dbReference type="EnsemblBacteria" id="AAZ68427">
        <property type="protein sequence ID" value="AAZ68427"/>
        <property type="gene ID" value="Ecaj_0384"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0384"/>
    <dbReference type="eggNOG" id="COG0257">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135723_3_2_5"/>
    <dbReference type="OrthoDB" id="2066169at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00251">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_L36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_L36_sp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="SSF57840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00828">
        <property type="entry name" value="RIBOSOMAL_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000302198" description="50S ribosomal protein L36">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00251"/>
        </source>
    </evidence>
    <sequence length="42" mass="4978" checksum="EF079A743B95EF1C" modified="2005-09-27" version="1">MKVIGSLKSAKVRDKDCRIVRRKGRIYVINKKNPRFKARQGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS82</accession>
    <name>Q3YS82_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304501.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0380"/>
    <dbReference type="EnsemblBacteria" id="AAZ68423">
        <property type="protein sequence ID" value="AAZ68423"/>
        <property type="gene ID" value="Ecaj_0380"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0380"/>
    <dbReference type="HOGENOM" id="CLU_155694_0_0_5"/>
    <dbReference type="OMA" id="DISSFGW"/>
    <dbReference type="OrthoDB" id="1599635at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011855">
        <property type="entry name" value="Phgtail_TP901_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06199">
        <property type="entry name" value="Phage_tail_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="129" mass="15132" checksum="48B9390088E429A0" modified="2005-09-27" version="1">MSIQLQIKDYNNSFFSLNNIKSLRISLHNKNEYIKNIHLSGWKSALENSGNKYIIFKIQGIVDCSPANQLLQQFSFNNDIIELKIIINEKETISAKCIIELYERYYESSNFDNFNTVFISSEKVIFNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS83</accession>
    <name>Q3YS83_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0379"/>
    <dbReference type="EnsemblBacteria" id="AAZ68422">
        <property type="protein sequence ID" value="AAZ68422"/>
        <property type="gene ID" value="Ecaj_0379"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0379"/>
    <dbReference type="HOGENOM" id="CLU_1515608_0_0_5"/>
    <dbReference type="OMA" id="LMSVIAH"/>
    <dbReference type="OrthoDB" id="1898227at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="182" mass="20881" checksum="E7317F01504136C1" modified="2005-09-27" version="1">MIDSCNNYHKLCKIDKEIITLLRIAQDESLLNAFSEVKHEASKEEQKLEIEEENNVLILENDDNNKDTEEIKNHIKLKLNILMSVIAHKMDPVQRAGETAQSNTKDSKKFGRSAAQDLSVVTTALFKMILNQIKLILEVAITNEKSENQQFGFQKELHIDPEQQSHLPNLVSQEDNQQSRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS86</accession>
    <name>Q3YS86_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenosylmethionine-8-amino-7-oxononanoate aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">7,8-diamino-pelargonic acid aminotransferase</fullName>
            <shortName evidence="4">DAPA AT</shortName>
            <shortName evidence="4">DAPA aminotransferase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">7,8-diaminononanoate synthase</fullName>
            <shortName evidence="4">DANS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Diaminopelargonic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bioA</name>
        <name evidence="6" type="ordered locus">Ecaj_0376</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate</text>
            <dbReference type="Rhea" id="RHEA:16861"/>
            <dbReference type="ChEBI" id="CHEBI:16490"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="EC" id="2.6.1.62"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.62" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0376"/>
    <dbReference type="EnsemblBacteria" id="AAZ68419">
        <property type="protein sequence ID" value="AAZ68419"/>
        <property type="gene ID" value="Ecaj_0376"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0376"/>
    <dbReference type="eggNOG" id="COG0161">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_4_3_5"/>
    <dbReference type="OMA" id="VAVKMCL"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00834">
        <property type="entry name" value="BioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005815">
        <property type="entry name" value="BioA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00508">
        <property type="entry name" value="bioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="119"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="310"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM" evidence="4">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="428" mass="47430" checksum="4DD88E762AC2F4F6" modified="2005-09-27" version="1">MLLNNMYINTCAWFNKGYQNIWMPYTQMKNSPLPLSVKSASGCYIILEDGKRLLDGISSWWSVCHGYSHPHIVQKVQDQVAKLSHVMFSGLAHEQSYVLASRLVKISPEQKKVFFSDSGSTAVEVAMKMAVQYHQNLGNKNKYNFVSFVNGYHGDTMGCMSISDPTKIHGTKFKKYHPSQFILPLPSTEEEIKNFANTIYSIRDQVAAVILEPILQAAGGMLIHSANILKKIYDIAKANNILFIADEVATGFGRTGTMFGCNQANIVPDIIVIGKALTGGFCTLAATLTTQEVYDAFLSDNIDDAFMHGPTFMANALACAAANASLDIFESQDLIRNIGLIENQLKSELGVFKQLDCVTDIRVKGATGVIELKDGLIDKHDIINKGVELNIWIRPLNNVIYVMPPFIINSNELTQLITSIYKILKSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS88</accession>
    <name>Q3YS88_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="5">1.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0374</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304495.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0374"/>
    <dbReference type="EnsemblBacteria" id="AAZ68417">
        <property type="protein sequence ID" value="AAZ68417"/>
        <property type="gene ID" value="Ecaj_0374"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0374"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_5"/>
    <dbReference type="OMA" id="KMPERDY"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011284">
        <property type="entry name" value="3oxo_ACP_reduc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01830">
        <property type="entry name" value="3oxo_ACP_reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="151"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="244" mass="26388" checksum="5828139227F373D1" modified="2005-09-27" version="1">MFNLSQKRFLITGASGGLGQAITKALSNAGATLCISGTRESVLEKIAEQYPNTYVLPCDLTNNEHVNSLINNACKAMSGIDGIVCNAGITLDKLTLRTTDEDWNKVINTNLTTTFKLNRNACRAMLKNNQGRVINISSVVAFTGNLGQANYSASKAGMIAMSKSIAREFASRNITVNCIAPGFIETPMTDVLSEEQRNNILTHIPMQRMGTPEEVAAAVLFLASDESRYITGQTLHVNGGMLMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS89</accession>
    <name>Q3YS89_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0373"/>
    <dbReference type="EnsemblBacteria" id="AAZ68416">
        <property type="protein sequence ID" value="AAZ68416"/>
        <property type="gene ID" value="Ecaj_0373"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0373"/>
    <dbReference type="HOGENOM" id="CLU_1048625_0_0_5"/>
    <dbReference type="OMA" id="FNYRRMK"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="265" mass="29750" checksum="CB272C12BDB42E7D" modified="2005-09-27" version="1">MFLFKKSTHKTKFYIALCSNVIYFGVFLFAIFIQLIRFPSKLSAKDLLLINVLMVLSARTFLFLLSSYELTCTYYNNKQSPLLKYKKAAHIAEVISTVLAIIIQIIAVVHVATGDLEIVSDKKSTIHTKGAVDFVSILIKLLIASPLLIYFYYNRMKENQHPTQQKKIKYLFYLSITSLVISSIAFIGKIINVLEQTQSFSLFNTENYSNSGPTNFPLGPIIRISCIALSIIILTIIVGIESSISPVLSDARTHSQYQSLEQSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS94</accession>
    <name>Q3YS94_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304489.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0368"/>
    <dbReference type="EnsemblBacteria" id="AAZ68411">
        <property type="protein sequence ID" value="AAZ68411"/>
        <property type="gene ID" value="Ecaj_0368"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0368"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1324675_0_0_5"/>
    <dbReference type="OMA" id="LMFGLEF"/>
    <dbReference type="OrthoDB" id="1310982at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230942" evidence="1">
        <location>
            <begin position="23"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="226" mass="26338" checksum="0ACBB55632BC6C56" modified="2005-09-27" version="1">MRIFLYLQIILLLFINSNMVFAEDAVNTSVRNFYIGGSYGYDKKTLELKVADKYLDFDFHVDDIIGYNIGLKLGYICPSASLNNFRTDLEFVYIAKREIIAENVEESLKKEILYWNDNKYKVFFNLYYDMGNFFSIKDLAFYFGSGVDIYGLIIDKLSKKSESSDIKSNFMLQNVIGLKYRLYNVAIYGGYKYFIGYTKSKKDNVIFKNCKILGSYGLIFGLEFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSA5</accession>
    <name>Q3YSA5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304478.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68400">
        <property type="protein sequence ID" value="AAZ68400"/>
        <property type="gene ID" value="Ecaj_0357"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0357"/>
    <dbReference type="HOGENOM" id="CLU_083847_0_0_5"/>
    <dbReference type="OMA" id="YFEVEQA"/>
    <dbReference type="OrthoDB" id="1916573at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021387">
        <property type="entry name" value="DUF3023"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11224">
        <property type="entry name" value="DUF3023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="225" mass="25143" checksum="693C7F50426470B0" modified="2005-09-27" version="1">MLGDEYLKKRRKHNNALCGALLMVNTMDLKAVYPIGITGSDGKMKVQITSTHQDKKLPLAGTGDTLFLLKCRLPSSTIEDDENLHKLIGGRRRCLTQYVKLHLYFLVEEKSVDDFMLRASLNFSQSIPAFTDFGDYGRFILARVRGGLYDDEFDEEKDLTNVSGLDASFMSEEEFQNYIKTQSSADKLESGIITSSDSTAIAVMTQLMDLSITGIESGMFPHDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSB4</accession>
    <name>Q3YSB4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304469.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0348"/>
    <dbReference type="EnsemblBacteria" id="AAZ68391">
        <property type="protein sequence ID" value="AAZ68391"/>
        <property type="gene ID" value="Ecaj_0348"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0348"/>
    <dbReference type="HOGENOM" id="CLU_531836_0_0_5"/>
    <dbReference type="OMA" id="EYVEMEG"/>
    <dbReference type="OrthoDB" id="1682133at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="535" mass="59884" checksum="AB06FDCCC0B31FD1" modified="2005-09-27" version="1">MNKSQSLYLFLFLLCVTILAFTAAILAFRFNDRLKIPYLNIIAAVVMSVLAVALLISVAHLVMHYQGTSVSVKDMPPRIAAGDQMVLFVPFTADQLSKLSPKNNIIDVKYDVVESGSSLAGKREFVLDITQSYGLCPTKKVQVVMDNNRQCQLLFTNEEISSQVNGPGMCFVVNGNTSIVKGNMQKPGFYIKHPEVKDGTLSLNCEYQKICTGSMEDLRYILRSECVRSGRTIYEMLLSNILTMCPNQKDKVIPSDASVTHSKDLWINTNSGRLALSLMKIISPDSTLINLEELKNDDEPLYQKITWIRNYLVTNGHNVSEASFEVIFKKASDIFYRRTAGMSEYGNLHSDRITLSGKKFSDILAEGMQLDEELLIIMAIASYSCEGTLDPYNTTNKFLREILMLDQQRSDSILKEIEEILGKHWYEIIYLNDLKFSGSKVCTQMKKLLQDGCDSATALEIVNAARAYMLEKQWSSVPITRGVGCIMNLGYLDRIRLCGNREVSVETQEEGVSSSIVEVVDTSSVQNLLAPGNMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSC1</accession>
    <name>Q3YSC1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68384">
        <property type="protein sequence ID" value="AAZ68384"/>
        <property type="gene ID" value="Ecaj_0341"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0341"/>
    <dbReference type="HOGENOM" id="CLU_1425955_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="190" mass="21534" checksum="B1F75730001C2569" modified="2005-09-27" version="1">MSFFTNKTNILILTVLVSMVLVFIIKCIYMRYNRVKYYDTLYEGDEQALDDQSECVNNNLPTNQNDSENTPRKVNEDIKDTLSSKENNFLGVFSDIHGCVNNNLQNDQSNSEIEKIDERVKGILSNKVKNDDLIIFEDVHEGINDRSAYENTCGDVNNNFLNSTGSDNSNNICTNSAAKAKKKKSFACLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-24" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSC5</accession>
    <name>RS15_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsO</name>
        <name type="ordered locus">Ecaj_0337</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304458.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSC5"/>
    <dbReference type="SMR" id="Q3YSC5"/>
    <dbReference type="STRING" id="269484.Ecaj_0337"/>
    <dbReference type="EnsemblBacteria" id="AAZ68380">
        <property type="protein sequence ID" value="AAZ68380"/>
        <property type="gene ID" value="Ecaj_0337"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0337"/>
    <dbReference type="eggNOG" id="COG0184">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148518_0_0_5"/>
    <dbReference type="OMA" id="FKTHVKD"/>
    <dbReference type="OrthoDB" id="1990141at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_S15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_S15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_S15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="S15_NS1_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="PTHR23321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="SSF47060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000115432" description="30S ribosomal protein S15">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <sequence length="93" mass="11060" checksum="D77CBEDC120777D2" modified="2005-09-27" version="1">MSITREKKSELISEYCLKKGDTGSSFVQCAILSERIRNLTEHLKTHKKDFHCRRGLMVLVYRRRNGLQYIKKKYGDDQYLALIKRLGIRDIFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSE7</accession>
    <name>Q3YSE7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">DNA primase</fullName>
            <ecNumber evidence="12">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dnaG</name>
        <name evidence="16" type="ordered locus">Ecaj_0314</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12 13 14">Binds 1 zinc ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer. Interacts with DnaB.</text>
    </comment>
    <comment type="domain">
        <text evidence="12">Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="12"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0314"/>
    <dbReference type="EnsemblBacteria" id="AAZ68358">
        <property type="protein sequence ID" value="AAZ68358"/>
        <property type="gene ID" value="Ecaj_0314"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0314"/>
    <dbReference type="eggNOG" id="COG0358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013501_5_3_5"/>
    <dbReference type="OMA" id="DEPILCF"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03364">
        <property type="entry name" value="TOPRIM_DnaG_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00974">
        <property type="entry name" value="DNA_primase_DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013264">
        <property type="entry name" value="DNA_primase_core_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037068">
        <property type="entry name" value="DNA_primase_core_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006295">
        <property type="entry name" value="DNA_primase_DnaG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036977">
        <property type="entry name" value="DNA_primase_Znf_CHC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030846">
        <property type="entry name" value="DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034151">
        <property type="entry name" value="TOPRIM_DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002694">
        <property type="entry name" value="Znf_CHC2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08275">
        <property type="entry name" value="Toprim_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01807">
        <property type="entry name" value="zf-CHC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002811">
        <property type="entry name" value="DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00400">
        <property type="entry name" value="ZnF_CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01391">
        <property type="entry name" value="dnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 12 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 12 13" id="KW-0639">Primosome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 12 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="15">
        <location>
            <begin position="254"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CHC2-type" evidence="12 14">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR002811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR002811-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAZ68358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="604" mass="68707" checksum="AEAE37F6F19094A1" modified="2005-09-27" version="1">MVHLIIIILLNLSIAYDMNVVDIIKSKVKLSDVVGQRVKLTKRGNSVIGLCPFHGEKTPSFTVSDEKGLYYCFGCGANGDVIQFTSDINALDFKGAVDYLAEMYSIDLPKSTSKKSSLIPLLDAATLWFEKQLFANRVAIEYIKSRKIFSDTVKKFRLGYAPAYGLSKYLLSQGFNINDIRSVGLLNSQDQDYFYNRIIFPICNSSGDTIGFGGRSIVDAQSPKYLNSRGNVFFQKRENLYASHFAVREVKKQGKIIVVEGYMDVLMLHQIGVCNVVGLLGTSMTSEHLMYLWEITSEIIIWMDGDVAGKMSSIKSALLALSLIKPGCVVKFVDVFTGMDPYDVCINGGVDSVNSLLDSAKLLSEFIWDYELAKISTDKSSVMPEQCVVLEARIKHYLDQIGDGNIAKYYKKYFYSQIRDLQYRRNESKPLANHSINRASNKLKYVNDEFLLEEYNQLRVMYVIMEFPELLDDPIIFDQFSKFNIKNKNVYKLQQNIINIKVTLCDHVMSKEMLLLELKKSNLEGVIDFISSKISASSFMSIQNRAHNIDIAKKEIEKIMLLDNLQCIQAEILDLRLHGKRDLAEKLSNQAQEIDSRLRELWNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSE8</accession>
    <name>Q3YSE8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0313</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304435.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0313"/>
    <dbReference type="EnsemblBacteria" id="AAZ68357">
        <property type="protein sequence ID" value="AAZ68357"/>
        <property type="gene ID" value="Ecaj_0313"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0313"/>
    <dbReference type="eggNOG" id="COG1565">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024840_3_0_5"/>
    <dbReference type="OMA" id="YYHPQRN"/>
    <dbReference type="OrthoDB" id="1220759at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PTHR12049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="335" mass="38444" checksum="3779ED281BD19C10" modified="2005-09-27" version="1">MYSYLKEVIFSSGGAISIEQFMQVALYDVHHGYYMTQMPFGAHGDFITAPEISQLFGEIIALWVLLSWQKIGAPSKFVVVELGPGRGTLINDVIRILKRFEQCYAAMSVYLVEISPVLENVQRDILKNEKVFWCRNVSDVPDCPILIIANEFFDALPIRQFTYFDNTWYETYVTLENDEFKIIYKSVDERFEVSSDIEKPVVETCNEAISIVKYIENKILQNSGAAVIIDYGYVNCPYKSTIQSVKCHKYNDLLKNIGKSDITSHVNFAVLRDSLSTLDSVIMNQRDFLYSFGIKERLRILIENATEVQRQNLITGFLRLTENMGSMFKVLLINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-04-07" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSF7</accession>
    <name>Q3YSF7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Response regulator receiver modulated diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0304</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304426.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0304"/>
    <dbReference type="EnsemblBacteria" id="AAZ68348">
        <property type="protein sequence ID" value="AAZ68348"/>
        <property type="gene ID" value="Ecaj_0304"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0304"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_28_5"/>
    <dbReference type="OMA" id="NDYFVYP"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="158"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="324"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="458" mass="52061" checksum="AEF79516865EBCEC" modified="2005-09-27" version="1">MTAKVLIVDDLPANIKLLQAKLMSEYYNVLTATNGMDAIKIAEEKHPDIILLDVMMPEMDGYETCKRLKSNPATTYIPIVMVTALDNTSDNRVSGLSYGADDFLTKPINDTALFARIRSLTRFKMVIDELRLRGKTNADMSVLNNNMMDYANKIDNASILIVDEDVIRSEHINNILQKSFQQTVVSHDIDQALNLSNIQNYDLIITDLEFSGDGLRLCSQLRNKIETRYTPILVLLDENEDPKLLSKAFDIGIHDYITTPVDSNELIARVNVQVKRKRYQDALRINVDTSMTMAITDPLTGCYNRRYFDMHFHNIINESLQKEKNLSLMILDIDHFKEVNDTFGHTVGDELLQQFKKRVSDNIRITDLLARFGGEEFVVILPDTTISTAEQIARRILTVIKDTPFKTSIGDINKTVSIGVVEVQKSDVDNIKQLIDRADKCLYEAKNTGRNKVVIYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSH5</accession>
    <name>Q3YSH5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TatD-related deoxyribonuclease</fullName>
            <ecNumber evidence="2">3.1.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304408.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0283"/>
    <dbReference type="EnsemblBacteria" id="AAZ68330">
        <property type="protein sequence ID" value="AAZ68330"/>
        <property type="gene ID" value="Ecaj_0283"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0283"/>
    <dbReference type="eggNOG" id="COG0084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_4_2_5"/>
    <dbReference type="OMA" id="PNEAPRI"/>
    <dbReference type="OrthoDB" id="915852at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015991">
        <property type="entry name" value="Hydrolase_TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00010">
        <property type="entry name" value="TIGR00010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01090">
        <property type="entry name" value="TATD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="260" mass="29364" checksum="7805C021A2E2EDB3" modified="2005-09-27" version="1">MIVDSHCHLNYFNPDEIKGIVLRAEENNVKLMQTVCTTMSEFSNLVSIAESYKQVYLSVGVHPTNSANGESVSVDELVTLAQHSKVIGLGETGLDFYKCSDIGEQERNFLAHINASRETRLPVIIHSRSADERMIEILESEMRESPFLGLMHCFASSRELAIKAIELGLYISFSGIVTFKNAKIVQEVAGFVPKDMVLVETDSPFLSPEPYRGQKNEPSRTKFVVEYLAKLWDMDIEEISNLTTNNFFRLFKKCSAHFHS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSI1</accession>
    <name>COAE_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dephospho-CoA kinase</fullName>
            <ecNumber evidence="1">2.7.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephosphocoenzyme A kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaE</name>
        <name type="ordered locus">Ecaj_0277</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3'-dephospho-CoA + ATP = ADP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18245"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoaE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304402.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSI1"/>
    <dbReference type="SMR" id="Q3YSI1"/>
    <dbReference type="STRING" id="269484.Ecaj_0277"/>
    <dbReference type="EnsemblBacteria" id="AAZ68324">
        <property type="protein sequence ID" value="AAZ68324"/>
        <property type="gene ID" value="Ecaj_0277"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0277"/>
    <dbReference type="eggNOG" id="COG0237">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057180_3_0_5"/>
    <dbReference type="OMA" id="NFTIRTG"/>
    <dbReference type="OrthoDB" id="1515383at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00356"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00152">
        <property type="entry name" value="TIGR00152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000243283" description="Dephospho-CoA kinase">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="DPCK" evidence="1">
        <location>
            <begin position="3"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00376"/>
        </source>
    </evidence>
    <sequence length="200" mass="23310" checksum="20BBBAEEF65718C0" modified="2005-09-27" version="1">MVIFGLTGGIGSGKSLVASYFSTFFKAKIFDADKVVHELYKYDSDVIRLVSEYFPDSVDNGIVDRNNLRQHFLTDNHLWVEFQSVIHAIVLKKKKDFIMLHNRRSVRYVVLDIPLLIESNFYSCCDFIIHVTTSRLLQMQRVLRRGLSIKEFESIRCKQLSESSRKKFANFTIRTGLSKKDILFQIKKIMLNVNNKCNMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSI9</accession>
    <name>Q3YSI9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Enoyl-[acyl-carrier-protein] reductase [NADH]</fullName>
            <ecNumber evidence="9">1.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0269</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:10240"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.9" evidence="9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0269"/>
    <dbReference type="EnsemblBacteria" id="AAZ68316">
        <property type="protein sequence ID" value="AAZ68316"/>
        <property type="gene ID" value="Ecaj_0269"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0269"/>
    <dbReference type="eggNOG" id="COG0623">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_10_1_5"/>
    <dbReference type="OMA" id="GILDMIH"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016631">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05372">
        <property type="entry name" value="ENR_SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014358">
        <property type="entry name" value="Enoyl-ACP_Rdtase_NADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43159">
        <property type="entry name" value="PTHR43159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000094">
        <property type="entry name" value="Enoyl-ACP_rdct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 9 11" id="KW-0520">NAD</keyword>
    <keyword evidence="5 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="75"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="202"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000094-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000094-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="280" mass="30732" checksum="38F7C6D620A4E184" modified="2005-09-27" version="1">MSRNKEILVLASDIMKGKKGLIIGIANDKSLAWGIAKTLAMHGADIAVTYLSDVFKKRIDPLVESIGVKLVLQCDVSKEDSVDTMFEQLNDKWENIDFLVHAVAFSDKNELKGRYIDTSLDNFLTSMHISCYSLTYLAKKVEKMMRNGGSILTLSYYGAQKVMPHYNVMGVCKAALEASVKYLAVDLGSQNIRVNAISAGPVRTLASSGIGDFHYILTWNKYNAPLRRNTTIEDVGGAALYLLSDLGKGTTGEILYVDSGYHAIGMKAIDAPDINYVEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSJ2</accession>
    <name>Q3YSJ2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4 5">Single-stranded DNA-binding protein</fullName>
            <shortName evidence="3">SSB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0266</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304391.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0266"/>
    <dbReference type="EnsemblBacteria" id="AAZ68313">
        <property type="protein sequence ID" value="AAZ68313"/>
        <property type="gene ID" value="Ecaj_0266"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0266"/>
    <dbReference type="eggNOG" id="COG0629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078758_0_2_5"/>
    <dbReference type="OMA" id="GNCIGRF"/>
    <dbReference type="OrthoDB" id="1942216at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00984">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="PTHR10302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002070">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="2 3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="Important for interaction with partner proteins" evidence="3">
        <location>
            <begin position="152"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="157" mass="17811" checksum="04E1C19C11DC2D72" modified="2005-09-27" version="1">MSTIGVNKVILIGNLGKDPDIRVMQNGKEMASFSIATSESWVDKTSGIRTEKTEWHNIVVFNEGLVKIIKNCVRKGSKIYIEGSLRTRKWTDQNNVDRYTTEVILQGFNSALYMLDAKNNTMDNNAIPNEYSTESFSKENFQDSSCDDIIIDDEIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSJ6</accession>
    <name>Q3YSJ6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="4">4.2.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Porphobilinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ecaj_0262</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 5-aminolevulinate = H(+) + 2 H2O + porphobilinogen</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304387.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0262"/>
    <dbReference type="EnsemblBacteria" id="AAZ68309">
        <property type="protein sequence ID" value="AAZ68309"/>
        <property type="gene ID" value="Ecaj_0262"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0262"/>
    <dbReference type="eggNOG" id="COG0113">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035731_0_0_5"/>
    <dbReference type="OMA" id="YQMDYAN"/>
    <dbReference type="OrthoDB" id="677575at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="PTHR11458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="10">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="10">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="330" mass="36991" checksum="6F3DD1C36C95F65E" modified="2005-09-27" version="1">MQFPSTRLRRKRLNGWLRNLVRETSLSINDLILPLFVHDRDGISEPIKQLPDIKRYSITELLNVVEQAANLGINAIALFPVVEKHLKSENAEEAFNPENLMYRAISEIKKYIPNIGIIADVALDPYTSSGHDGIVVNDKIDNDLTIKALCKQALVLAAAGCDIVAPSDMMDGRIKEIRNVLDNCTFQDVCILSYSVKYCSSFYKPFREAVGSVSRSQTIDKSTYQLDISNINEAILEVQMDIDESADIVMIKPGMPYLDVIKCISSRYQTPVFAYQVSGEYAMIKAASQHHLLDYDLAIYESLLGFKRAGAKSIITYAALDIANMLKNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSJ7</accession>
    <name>Q3YSJ7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="3">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0261</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + deamido-NAD(+) + H2O + L-glutamine = AMP + diphosphate + H(+) + L-glutamate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304386.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0261"/>
    <dbReference type="EnsemblBacteria" id="AAZ68308">
        <property type="protein sequence ID" value="AAZ68308"/>
        <property type="gene ID" value="Ecaj_0261"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0261"/>
    <dbReference type="eggNOG" id="COG0171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022313_0_0_5"/>
    <dbReference type="OMA" id="YKTRVYE"/>
    <dbReference type="OrthoDB" id="1152435at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 4" id="KW-0520">NAD</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NAD_synthase" evidence="5">
        <location>
            <begin position="242"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="505" mass="57671" checksum="4D479F8E20B779DD" modified="2005-09-27" version="1">MYYPSIFIAQLNYNPENIDYNYDKITKLYTESVKKAADLAIFARYSIPGYIQKIPYLSKDFFNQCDHAINKLALQTYNKNTAIIIGSIKQQNNKIFEIIYLIKNGKIEALIEFSTEYQFSDIAATFTINKINVSLLPISKIPTLPSYTKDTLLLIMDNTPYTQETNSRDNMISKLTCDKYAVYINQVGGYNNRVFHGKSFFTFNGQYNFLNMWQEDSNIYSIDFAKQTHYMTTCDNIESDYQALMLALRDYVYKNSMASIIIGLSGGIDSALVASIAADALGPKNVHSFMLPTKYTSQTSITDSQECAKRLEISYDILPIEKIFQNSLAVLQNTFANTEEDVTEENLQARIRGIILMALSNKFKFMLLSTGNKSEIFVGYATLYGDMCGGFAPIKDIYKTKVYELSKWRNKNIPKNSLCQKIDVIPSQIIEKHPSAELCYNQKDQDTLPDYTTLDSVLTLLIDKNYSVEDVIKKGYNFQDVNYIVHLIKKSQFKREQAPTGPLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSK8</accession>
    <name>Q3YSK8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="9">2-octaprenylphenol hydroxylase</fullName>
            <ecNumber evidence="9">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis [regulation].</text>
    </comment>
    <dbReference type="EC" id="1.14.13.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304375.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0250"/>
    <dbReference type="EnsemblBacteria" id="AAZ68297">
        <property type="protein sequence ID" value="AAZ68297"/>
        <property type="gene ID" value="Ecaj_0250"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0250"/>
    <dbReference type="eggNOG" id="COG0661">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006533_0_0_5"/>
    <dbReference type="OMA" id="RRDYKRV"/>
    <dbReference type="OrthoDB" id="678616at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010232">
        <property type="entry name" value="UbiB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01982">
        <property type="entry name" value="UbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="110"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="480" mass="55681" checksum="2442289207E6107D" modified="2005-09-27" version="1">MLSIFSSIISLLNILSILVRYNVLPYTYFFADRSIRRKSQGERVTMALQRLGPIFIKFGQSMSSRADIIGEEIANHLLCLCDKLPAFPYADVVRIIEEDFKCNISEMFCEFEQKPIAAASIAQVHKAKDIDGNVRAVKVLRPKIEKIFERDIKLMFWLARLAQYFSRFRRFQLLQLVQTFREICRMEIDLRFEAANAEELRSNIQDDDGCYVPEVDWTKTTRRVLTLEWVDAVPIYSVYKSENCETLVRNLIFCFCNQVYRDRFFHADMHPGNLMVDTKGRIVIIDFGIVGRLDKATCMYVTEILVGFLKKDYQYVADIHYRAGYVDAKYSSFITACRAIGEPIVGKNAENISISMLLTQLFKITSDFDMKLQPQLFLLQKTTVLVEGVCRQLCPEINMWKVAELWMEKYSIEKNKYIQKLKRSYICQTINDIPSFMKKVDKSLDVISNYQELNKANIGINRDIIWALIVGGLIIKLIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSL0</accession>
    <name>Q3YSL0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Periplasmic solute binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0248</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the bacterial solute-binding protein 9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304373.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0248"/>
    <dbReference type="EnsemblBacteria" id="AAZ68295">
        <property type="protein sequence ID" value="AAZ68295"/>
        <property type="gene ID" value="Ecaj_0248"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0248"/>
    <dbReference type="eggNOG" id="COG4531">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074098_0_0_5"/>
    <dbReference type="OMA" id="FHEAFDY"/>
    <dbReference type="OrthoDB" id="1703187at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006128">
        <property type="entry name" value="Lipoprotein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006127">
        <property type="entry name" value="ZnuA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01297">
        <property type="entry name" value="ZnuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00690">
        <property type="entry name" value="ADHESNFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="287" mass="33092" checksum="77C5EC8BEDE1B532" modified="2005-09-27" version="1">MKHIVFLLFFYTLLDSTIGYSVPRIIATINPIYSLVNAVTNGVTKPILLINESVSIHDYMLKPSDKRKLQDSDIIFYVDNHLETFINKIHNKKLVRLSEVVELLPSRYDTNFSYKIHTTSNDLHIWLNPENAKSIVKHIMLVLCQTDPENAEKYTNNANLTLTQITQQTETISNMLSSVKTKPYIVTHDAYQYFEKYFGLNFIAALSSSHDTNISVKRLMHIKKIVTQNKIKCIFSESSNDKVKNLFSKYQVKFQVLDPIGNTSEDNAYFNIMQNITDNLVHCLSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSL3</accession>
    <name>Q3YSL3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0243</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of an ABC transporter complex. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304370.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0243"/>
    <dbReference type="EnsemblBacteria" id="AAZ68292">
        <property type="protein sequence ID" value="AAZ68292"/>
        <property type="gene ID" value="Ecaj_0243"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0243"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_5"/>
    <dbReference type="OMA" id="RNLACGY"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015408">
        <property type="term" value="F:ABC-type ferric iron transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03259">
        <property type="entry name" value="ABC_Carb_Solutes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015853">
        <property type="entry name" value="ABC_transpr_FbpC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="4"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="340" mass="38283" checksum="EC736E5537B7D090" modified="2005-09-27" version="1">MVGLQLENVFYKYKKGQFLLNDININCKQGEVVCLLGPSGCGKSTILKLIAGLEDLQYGSICINGKVVVDSNFCMPTEKRNVGLIFQHPSLFPHQTVIENVMFAVKDSSKTQKFNIALSILQSINMVAYKDMYPDMLSGGQQQLITIARVIAQRPEVILLDEPFSSLDTALRINIRRHVLSLLKSKKITVLLVTHDPEEALEVADTVYIMRDGYIVQHGTPYEIYYNPRDHMLAQFFGEVNHFVCTVKDYCIELPIGKIPAQSFANGEEVVVCIRPEAIISHSNNGIKAVVKQIKFFNNMVCVCVGDCLYWMRFANVVLPQIGDTIFILLDLNKILLFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSL4</accession>
    <name>Q3YSL4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DsbC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304369.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0242"/>
    <dbReference type="EnsemblBacteria" id="AAZ68291">
        <property type="protein sequence ID" value="AAZ68291"/>
        <property type="gene ID" value="Ecaj_0242"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0242"/>
    <dbReference type="eggNOG" id="COG4233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134301_0_0_5"/>
    <dbReference type="OMA" id="GWHIYYK"/>
    <dbReference type="OrthoDB" id="1684599at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028250">
        <property type="entry name" value="DsbDN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036929">
        <property type="entry name" value="DsbDN_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11412">
        <property type="entry name" value="DsbC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DsbC" evidence="1">
        <location>
            <begin position="38"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="169" mass="19813" checksum="FEF9CD4746F90A3B" modified="2005-09-27" version="1">MLKFILTILITLFVTNECYASNVINLVNMNLLIGSLDMKNKTIEAAIKVTIQNGWHIYYHTPGDLGLPTVFQWQDDIFKKVDIHWPKPLQHTDTVSNNTFHSNIYKDLVVFPISLVFKDNKELNTTLRIRYAVCKDVCIPREKVIILNQILQDYYNKQIIQIINFWKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSL6</accession>
    <name>Q3YSL6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Biotin--[biotin carboxyl-carrier protein] ligase</fullName>
            <ecNumber evidence="3">6.3.4.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0240</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + biotin + L-lysyl-[protein] = AMP + diphosphate + H(+) + N(6)-biotinyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:11756"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.15"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.3.4.15" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304367.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0240"/>
    <dbReference type="EnsemblBacteria" id="AAZ68289">
        <property type="protein sequence ID" value="AAZ68289"/>
        <property type="gene ID" value="Ecaj_0240"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0240"/>
    <dbReference type="eggNOG" id="COG0340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051096_3_0_5"/>
    <dbReference type="OMA" id="AVWKHIE"/>
    <dbReference type="OrthoDB" id="1842956at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin-[acetyl-CoA-carboxylase] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="4">
        <location>
            <begin position="4"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="247" mass="28397" checksum="CCB3A1B52A399D48" modified="2005-09-27" version="1">MKSNINLPNGYSLEHHKILYSTNYEAIKLAECGAKDGTIIISDEQIKGRGRYERIWHSPKGNLYFSIIITKFVEIFPIPFIAALSVGKTLEEILENKEISKVQYKWPNDVLINSKKISGILLESKTLNNAPQKWIVVGIGINMYKAPEYATTIKQHNINFTLSNLELLEKVINHFNNLRKYYINYGFTKIKDLWLKQTCHLNSKITVKSHNNTYTGKFVTIDMKGNMIMQQEDKTTCITSGEIFNIT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-02-21" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSL9</accession>
    <name>HTPG_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein HtpG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heat shock protein HtpG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">High temperature protein G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">htpG</name>
        <name type="ordered locus">Ecaj_0237</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone. Has ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304364.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSL9"/>
    <dbReference type="SMR" id="Q3YSL9"/>
    <dbReference type="STRING" id="269484.Ecaj_0237"/>
    <dbReference type="PRIDE" id="Q3YSL9"/>
    <dbReference type="EnsemblBacteria" id="AAZ68286">
        <property type="protein sequence ID" value="AAZ68286"/>
        <property type="gene ID" value="Ecaj_0237"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0237"/>
    <dbReference type="eggNOG" id="COG0326">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006684_3_0_5"/>
    <dbReference type="OMA" id="MRRMKEM"/>
    <dbReference type="OrthoDB" id="246194at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="PTHR11528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="SSF110942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000224204" description="Chaperone protein HtpG">
        <location>
            <begin position="1"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="A; substrate-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="B" evidence="1">
        <location>
            <begin position="335"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="C" evidence="1">
        <location>
            <begin position="559"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00505"/>
        </source>
    </evidence>
    <sequence length="637" mass="72723" checksum="96EBF42BED5EB620" modified="2005-09-27" version="1">MQDVVNSEKLKFDAEVGKVLKLVIHSLYTNKDIFLRELISNASDACDKLRYESLSNQDLIDGDTDFKIVISVDQDKNRLYISDNGIGMNRQDLIDNLGTIAHSGTQRFLDAINNETSSQGVVELIGKFGVGFYSAFMVASEVIVESRKAGESIGYQWRSAGDGEFIISQLEDGQFPRGTKITLILKTEESEFVDKFRIEHIVTTYSYHINYPVYFLNDAGEEEKLNSDAAIWTKSKDEISAQEHQNFFRSVAHVGGEPWMILHNKNEGVIEYTNLLYIPSIKPFDLFHPDRKCSVKLYVNKVFITEDNVQIIPQYLRFLKGIIDSSDLPLNISRETLQNNKVIEKIKQSIVKRVLSELKKKAENDINDYKKFWENFGSVLKEGLCESMNTEFREELLSACRFYSTNSDDSLISLEDYIERMKEGQDNIYYLTGNDLDSIKKSPQLEGFVSRGIEVILLIDPVDDFWTNVVTDYQRVPLKSVIRADEDLEKLAHLKKDEEVGESKDENPDSKEKVDAFVKYAIQVLDKLVSGVRVSKKLTNSPVCLAVADGSMDIRMERFLREQKQLNYKSTKILEINPKHPIVSRMIDEYANTGENAVLDNMLHLLLGQACILEGEELEDVSSFAERMNNVLVKVYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSN2</accession>
    <name>Q3YSN2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Disulfide bond formation protein DsbB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0224</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0224"/>
    <dbReference type="EnsemblBacteria" id="AAZ68273">
        <property type="protein sequence ID" value="AAZ68273"/>
        <property type="gene ID" value="Ecaj_0224"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0224"/>
    <dbReference type="eggNOG" id="COG1495">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098660_0_1_5"/>
    <dbReference type="OMA" id="EWGFWPG"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003752">
        <property type="entry name" value="DiS_bond_form_DsbB/BdbC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023380">
        <property type="entry name" value="DsbB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024199">
        <property type="entry name" value="Uncharacterised_DsbB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02600">
        <property type="entry name" value="DsbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033913">
        <property type="entry name" value="S-S_format_DsbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158442">
        <property type="entry name" value="SSF158442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="159" mass="17951" checksum="7116F0A760FDDFF1" modified="2005-09-27" version="1">MGIVKNYGVTLLFIASVVALSVAYISQYLFGMLPCKLCLYERIPYFINIGLFIVYLFKPSKIVFFVMCLCYVCNIFISGYHVALEHDWVADIVGCTDNTQSLSFEDIKSSLLDVNVIVSCARPSVVFMGLSMAECNLIYCVLCFTLGIHLFVKQYVTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSP6</accession>
    <name>Q3YSP6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ecaj_0210</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0053 family. Hemolysin C subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304337.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0210"/>
    <dbReference type="EnsemblBacteria" id="AAZ68259">
        <property type="protein sequence ID" value="AAZ68259"/>
        <property type="gene ID" value="Ecaj_0210"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0210"/>
    <dbReference type="eggNOG" id="COG4536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_4_1_5"/>
    <dbReference type="OMA" id="TIGRQHP"/>
    <dbReference type="OrthoDB" id="1155802at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="122"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="12">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="206"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="274"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="428" mass="47894" checksum="37A7AE67BEEA30FF" modified="2005-09-27" version="1">MGLLLTSILSILILLILSAFFSAAETSITSISSSLVHRLMLQGNKRAKIINTLSQKKKIVINTVLIGNTIINITASSIATAIFIEILGPQGILFSTVIMTFFILIFSEALPKSYAILNPEKIALLISCPLSFCVLVLSPITLSIQYMIDFILKILGMHKDKEIISAAEAMRDLISLHDSKGTMLKQDLDMLSSILDLAETEISQVMTHRKNILAFNIDTNINDLIKKILASSHSRIPLWKNQEDQIIGVVHVKDVITLIREKGKSITQEDIHRIMTKPWFVPDTTLLSVQLHNFLKNRKHLALVIDEYGALQGIVTLEDVIEEIVGDITDEHDITTEAPIKQISENIYHINGFTSIRDINRQLRWNLPDEEASTLAGAIVYEVERIPEEGEEFLLYGLAFKILKKSGHTISSIQVDISPTSIEKHKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSP7</accession>
    <name>Q3YSP7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thiamine-phosphate synthase</fullName>
            <ecNumber evidence="8">2.5.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Thiamine-phosphate pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ecaj_0208</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 8">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H(+) = diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 9">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the thiamine-phosphate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.3" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304336.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0208"/>
    <dbReference type="EnsemblBacteria" id="AAZ68258">
        <property type="protein sequence ID" value="AAZ68258"/>
        <property type="gene ID" value="Ecaj_0208"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0208"/>
    <dbReference type="eggNOG" id="COG0352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018272_8_0_5"/>
    <dbReference type="OMA" id="GFYQIVP"/>
    <dbReference type="OrthoDB" id="461201at2"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00141"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034291">
        <property type="entry name" value="TMP_synthase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="SSF51391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00693">
        <property type="entry name" value="thiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="5 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TMP-TENI" evidence="10">
        <location>
            <begin position="162"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="349" mass="39935" checksum="992355263C9BC647" modified="2005-09-27" version="1">MFECNEIYIIGKDDLVQYSHTSQLKNLDDIIKSVSLLYSIKKKSIYAIDVTDRSNNKCLDYYFNGQEGLWISYKSEPYNDNAKFLLEYKKACTLESKCLNPLDFQKDTLVIARACTNQSVESTSFLDEKCFPNIMIDKDTRFEKNFPAIIDPIRFYQIVPDLYWLRYVINLGVKVVQLRIKDKPIENIENQIKEGVCLANQSNIKLFINDYWQLAIKYKAYGVHLGQKDLKDANFNEIFNAGLRLGISTHCYHELAIARYLRPSYIAFGPIFTTTLKNMDFMPQGVDLLSYWVKNLRSSIIAIGGINLLNVDSVIKTGVNGVAVVSAVLSNNNPAEATYEFIQKCGSIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSP8</accession>
    <name>PDRP_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative pyruvate, phosphate dikinase regulatory protein</fullName>
            <shortName evidence="1">PPDK regulatory protein</shortName>
            <ecNumber evidence="1">2.7.11.32</ecNumber>
            <ecNumber evidence="1">2.7.4.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Ecaj_0207</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional serine/threonine kinase and phosphorylase involved in the regulation of the pyruvate, phosphate dikinase (PPDK) by catalyzing its phosphorylation/dephosphorylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ADP + N(tele)-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] = AMP + H(+) + N(tele)-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase]</text>
            <dbReference type="Rhea" id="RHEA:43692"/>
            <dbReference type="Rhea" id="RHEA-COMP:10650"/>
            <dbReference type="Rhea" id="RHEA-COMP:10651"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:83586"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.32"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + N(tele)-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] + phosphate = diphosphate + N(tele)-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase]</text>
            <dbReference type="Rhea" id="RHEA:43696"/>
            <dbReference type="Rhea" id="RHEA-COMP:10650"/>
            <dbReference type="Rhea" id="RHEA-COMP:10651"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:83586"/>
            <dbReference type="EC" id="2.7.4.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyruvate, phosphate/water dikinase regulatory protein family. PDRP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.32" evidence="1"/>
    <dbReference type="EC" id="2.7.4.27" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304335.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSP8"/>
    <dbReference type="SMR" id="Q3YSP8"/>
    <dbReference type="STRING" id="269484.Ecaj_0207"/>
    <dbReference type="EnsemblBacteria" id="AAZ68257">
        <property type="protein sequence ID" value="AAZ68257"/>
        <property type="gene ID" value="Ecaj_0207"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0207"/>
    <dbReference type="eggNOG" id="COG1806">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046206_2_0_5"/>
    <dbReference type="OMA" id="YAQCEFE"/>
    <dbReference type="OrthoDB" id="980472at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016776">
        <property type="term" value="F:phosphotransferase activity, phosphate group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00921">
        <property type="entry name" value="PDRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005177">
        <property type="entry name" value="Kinase-pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026565">
        <property type="entry name" value="PPDK_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31756">
        <property type="entry name" value="PTHR31756"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03618">
        <property type="entry name" value="Kinase-PPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000196652" description="Putative pyruvate, phosphate dikinase regulatory protein">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="153"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00921"/>
        </source>
    </evidence>
    <sequence length="273" mass="31595" checksum="10B3FBA78A635170" modified="2005-09-27" version="1">MNASVVLNLHLISDSTCETVAAVAKSALEHFRSIEVNEFVWSFINSCEQIDKIMSLIEHDKYNFIMYTMFDDDIRRYLKQKAEVQEIPCIPVLSRVIRELSCYLHVKKDPYVNTGMGLDDEYFTRIDAINYTIAHDDGQNLWDIDKADIIILGVSRTSKSPTSIYLAYRGYRVVNIPLVNSINLSVDLSSMKNKLIVGLTIDIDRLIEIRKTRLVSMRNQNNCQYVDYEHVLVEIKETKRICMQNGWPIIDVTQKSVEEIAATIIQYFNKMQH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSQ3</accession>
    <name>CLPP_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="1">3.4.21.92</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Endopeptidase Clp</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clpP</name>
        <name type="ordered locus">Ecaj_0202</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).</text>
            <dbReference type="EC" id="3.4.21.92"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304330.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSQ3"/>
    <dbReference type="SMR" id="Q3YSQ3"/>
    <dbReference type="STRING" id="269484.Ecaj_0202"/>
    <dbReference type="MEROPS" id="S14.001"/>
    <dbReference type="EnsemblBacteria" id="AAZ68252">
        <property type="protein sequence ID" value="AAZ68252"/>
        <property type="gene ID" value="Ecaj_0202"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0202"/>
    <dbReference type="eggNOG" id="COG0740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058707_3_3_5"/>
    <dbReference type="OMA" id="RDYWMKA"/>
    <dbReference type="OrthoDB" id="1728970at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00493">
        <property type="entry name" value="clpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <feature type="chain" id="PRO_0000226447" description="ATP-dependent Clp protease proteolytic subunit">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00444"/>
        </source>
    </evidence>
    <sequence length="200" mass="22307" checksum="297C9DA9DD5518C7" modified="2005-09-27" version="1">MTLVPMVVEQTSRGERAYDIYSRLLKERIIFITGPIEDQMASLVVAQLVFLEAENPEKEICMYINSPGGVVTAGLSIYDTMQYIKPKVSTLCLGQAASMGSLLLAAGEPGMRYALPNSRIMIHQPSGGFQGQATDIEIHAKEILDIKSRLNDIYVKHTGRDLPEVVANMERDNFMRAEKAKDFGIIDKVIEKRLDIEESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSQ8</accession>
    <name>Q3YSQ8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304325.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0197"/>
    <dbReference type="EnsemblBacteria" id="AAZ68247">
        <property type="protein sequence ID" value="AAZ68247"/>
        <property type="gene ID" value="Ecaj_0197"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0197"/>
    <dbReference type="eggNOG" id="COG2975">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168040_0_1_5"/>
    <dbReference type="OMA" id="AIQQAWI"/>
    <dbReference type="OrthoDB" id="1860577at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007479">
        <property type="entry name" value="ISC_FeS_clus_asmbl_IscsX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036762">
        <property type="entry name" value="IscX-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37532">
        <property type="entry name" value="PTHR37532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04384">
        <property type="entry name" value="Fe-S_assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039003">
        <property type="entry name" value="IscX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140319">
        <property type="entry name" value="SSF140319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03412">
        <property type="entry name" value="iscX_yfhJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="66" mass="7829" checksum="7481CEAFF5066D78" modified="2005-09-27" version="1">MKWNDIESVAMSLEDHYPRHDIFGTRFTDLRLMILGLPDFDDDKDGCNEKILESIQLAWSEERENK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-10" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSR0</accession>
    <name>RS4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsD</name>
        <name type="ordered locus">Ecaj_0194</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304323.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSR0"/>
    <dbReference type="SMR" id="Q3YSR0"/>
    <dbReference type="STRING" id="269484.Ecaj_0194"/>
    <dbReference type="EnsemblBacteria" id="AAZ68245">
        <property type="protein sequence ID" value="AAZ68245"/>
        <property type="gene ID" value="Ecaj_0194"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0194"/>
    <dbReference type="eggNOG" id="COG0522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092403_0_0_5"/>
    <dbReference type="OMA" id="PVICDEQ"/>
    <dbReference type="OrthoDB" id="1211060at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_B">
        <property type="entry name" value="Ribosomal_S4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005709">
        <property type="entry name" value="Ribosomal_S4_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="PTHR11831:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01017">
        <property type="entry name" value="rpsD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000293272" description="30S ribosomal protein S4">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="91"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <sequence length="202" mass="23086" checksum="523F161442D93F56" modified="2005-09-27" version="1">MAVQRKYRASRRLGVSLWGRSKDPFNTRNYPPGQHGNMGYKKPSDFGKQFAAHKKFKFYYAISSKQMRNVFLKAYKKRGDTGDNFVGLLESRLSSILYNSGLVPTIFSARQLISHKHVLVNGKTVNISSYIVKVGDVVTLKEKAKNLPAVIAAIQSQEHKVPDYLEVDTQEKSVRYLRVPKYCEVPYPATMEVNLVIEFYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSR5</accession>
    <name>Q3YSR5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase cytochrome b556 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">Ecaj_0189</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Membrane-anchoring subunit of succinate dehydrogenase (SDH).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="16">The heme is bound between the two transmembrane subunits.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur protein, plus two membrane-anchoring proteins, SdhC and SdhD. The complex can form homotrimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome b560 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0189"/>
    <dbReference type="EnsemblBacteria" id="AAZ68240">
        <property type="protein sequence ID" value="AAZ68240"/>
        <property type="gene ID" value="Ecaj_0189"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0189"/>
    <dbReference type="eggNOG" id="COG2009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094691_1_1_5"/>
    <dbReference type="OMA" id="GYTWALM"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045281">
        <property type="term" value="C:succinate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000104">
        <property type="term" value="F:succinate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018495">
        <property type="entry name" value="Succ_DH_cyt_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978">
        <property type="entry name" value="PTHR10978"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000178">
        <property type="entry name" value="SDH_cyt_b560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="SSF81343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01001">
        <property type="entry name" value="SDH_CYT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 16" id="KW-0349">Heme</keyword>
    <keyword evidence="13 16" id="KW-0408">Iron</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <note>ligand shared with second transmembrane subunit</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000178-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AAZ68240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="136" mass="15975" checksum="6398ED57946594A7" modified="2005-09-27" version="1">MFHFFNLIMSKVRPLSPHLQIYKVVYKLSIMHRFTGMLLFVGLFIISWGIIAAFLFPKIIYEIYNFFSTCYLALYSFKLIAFIWLNIFSYHYLNGIRHLLWDLGFCLTKSAVKVTGIIVVCLCIISSFILYSLFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSS5</accession>
    <name>Q3YSS5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide deformylase</fullName>
            <shortName evidence="2">PDF</shortName>
            <ecNumber evidence="2">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">def</name>
        <name evidence="3" type="ordered locus">Ecaj_0179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304308.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0179"/>
    <dbReference type="EnsemblBacteria" id="AAZ68230">
        <property type="protein sequence ID" value="AAZ68230"/>
        <property type="gene ID" value="Ecaj_0179"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0179"/>
    <dbReference type="eggNOG" id="COG0242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061901_2_0_5"/>
    <dbReference type="OMA" id="ANDMMKV"/>
    <dbReference type="OrthoDB" id="1649129at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="194" mass="21801" checksum="C26F91197BD6B476" modified="2005-09-27" version="1">MNILSVDNVQDLRNLHAISHPIEKIDQEIIALANDMMKVMEDSKTVGLSAVQLGNHSRMFTINMFSGLFDVTQDIKVLSGHHSLHGKNMVCINPEVLSFSAETVDLFEGCSSAKSYGLINITRPKHMDFRYTDLLGNKCVVRVYGWLSRCIQHELDHLNGILLANVVDNIKNNCVNSISYEDHSVIHILLVNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSS8</accession>
    <name>Q3YSS8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68227">
        <property type="protein sequence ID" value="AAZ68227"/>
        <property type="gene ID" value="Ecaj_0176"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0176"/>
    <dbReference type="HOGENOM" id="CLU_1330213_0_0_5"/>
    <dbReference type="OMA" id="NIILVFC"/>
    <dbReference type="OrthoDB" id="1521099at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="198" mass="22407" checksum="94518F6F66719808" modified="2005-09-27" version="1">MQVIYVINVVLIFFITGVLLHDVYSISGDKVVEFNLLDNVEFVKSSDNFSGDLKNKISLVQVDNSAISVVDLQDWYYRLLKQEESLKSKETLLRDVEKHNNEQILYLENVKKVLASLVKVDDQKERIHSLVKIYENMPVELAAEIFGLLDINSLMSIANNIDRVTLSNILLHVSPNVTEMIKKASTSVSKCDCSSNIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-23" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YST8</accession>
    <name>RL1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplA</name>
        <name type="ordered locus">Ecaj_0166</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304295.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YST8"/>
    <dbReference type="SMR" id="Q3YST8"/>
    <dbReference type="STRING" id="269484.Ecaj_0166"/>
    <dbReference type="EnsemblBacteria" id="AAZ68217">
        <property type="protein sequence ID" value="AAZ68217"/>
        <property type="gene ID" value="Ecaj_0166"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0166"/>
    <dbReference type="eggNOG" id="COG0081">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_0_0_5"/>
    <dbReference type="OMA" id="GWTDVDV"/>
    <dbReference type="OrthoDB" id="1671455at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_B">
        <property type="entry name" value="Ribosomal_L1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005878">
        <property type="entry name" value="Ribosom_L1_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_L1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_L1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_L1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_L1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_L1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="SSF56808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01169">
        <property type="entry name" value="rplA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000307651" description="50S ribosomal protein L1">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <sequence length="220" mass="24067" checksum="6A4F1F33830F536A" modified="2005-09-27" version="1">MSSLVSNEVYDVESGLRKVIESAKANFCESVDVAINLNINSSKSDEQVRGCVVLPKGLGREVRVAVFAKGGHLEMAREAMANIVGDEELIEEVKKKQCKLDVDWCLTTPDFMASVSSIAKILGPKGLMPNPKFNTVTFELAKAIKIIKSGQIRFKSDKTGIVHAKIGNVKFSIEDLLENFNAVINAVKQCKPASIKGLYFKDVFVISTMGKSVKVENLNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSY7</accession>
    <name>LOLD_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipoprotein-releasing system ATP-binding protein LolD</fullName>
            <ecNumber evidence="1">7.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lolD</name>
        <name type="ordered locus">Ecaj_0117</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex LolCDE involved in the translocation of mature outer membrane-directed lipoproteins, from the inner membrane to the periplasmic chaperone, LolA. Responsible for the formation of the LolA-lipoprotein complex in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (LolD) and two transmembrane proteins (LolC and LolE).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Lipoprotein translocase (TC 3.A.1.125) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304246.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSY7"/>
    <dbReference type="SMR" id="Q3YSY7"/>
    <dbReference type="STRING" id="269484.Ecaj_0117"/>
    <dbReference type="EnsemblBacteria" id="AAZ68168">
        <property type="protein sequence ID" value="AAZ68168"/>
        <property type="gene ID" value="Ecaj_0117"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0117"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="FVYQSHR"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015854">
        <property type="entry name" value="ABC_transpr_LolD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220:SF654">
        <property type="entry name" value="PTHR24220:SF654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51244">
        <property type="entry name" value="LOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000272078" description="Lipoprotein-releasing system ATP-binding protein LolD">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="5"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01708"/>
        </source>
    </evidence>
    <sequence length="226" mass="25031" checksum="E423A576FFD7E1F7" modified="2005-09-27" version="1">MSTVFALSNISKFFGKNNELPIITNANIKIKKGEIVALIGRSGSGKSTLLHIAGLLDTPSSGNIFINNIECSNKTSDKDKTFLRRHFLGFIYQFHHLLQEFSVLENVMLPQIIIGKSKSIAKKNAMEILERVKLQDKFSMSISQLSGGERQRVAIARSLINCPLIVLADEPTGSLDNNTAFEVFSLLQEYAKEKNIAIFLATHNKSLAQKACRIVEINSCMLSSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSZ2</accession>
    <name>Q3YSZ2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Riboflavin biosynthesis protein RibD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="11">Diaminohydroxyphosphoribosylaminopyrimidine deaminase</fullName>
                <shortName evidence="11">DRAP deaminase</shortName>
                <ecNumber evidence="11">3.5.4.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">Riboflavin-specific deaminase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="11">5-amino-6-(5-phosphoribosylamino)uracil reductase</fullName>
                <ecNumber evidence="11">1.1.1.193</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">HTP reductase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ecaj_0112</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H(+) + H2O = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5-amino-6-(5-phospho-D-ribosylamino)uracil + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17845"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58421"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="EC" id="1.1.1.193"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 14">Binds 1 zinc ion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">In the C-terminal section; belongs to the HTP reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">In the N-terminal section; belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.26" evidence="11"/>
    <dbReference type="EC" id="1.1.1.193" evidence="11"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304241.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0112"/>
    <dbReference type="EnsemblBacteria" id="AAZ68163">
        <property type="protein sequence ID" value="AAZ68163"/>
        <property type="gene ID" value="Ecaj_0112"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0112"/>
    <dbReference type="eggNOG" id="COG0117">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1985">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036590_1_1_5"/>
    <dbReference type="OMA" id="PYIILKW"/>
    <dbReference type="OrthoDB" id="1775351at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00401"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008835">
        <property type="term" value="F:diaminohydroxyphosphoribosylaminopyrimidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004794">
        <property type="entry name" value="Eubact_RibD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006769">
        <property type="entry name" value="RibD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00326">
        <property type="entry name" value="eubact_ribD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11 13" id="KW-0521">NADP</keyword>
    <keyword evidence="11 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="9 11 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="15">
        <location>
            <begin position="3"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR006769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAZ68163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="361" mass="40130" checksum="661A34C7F69A7BF2" modified="2005-09-27" version="1">MMHDDKKFMSLALRIARRGLGNVFPNPAVGCIVVNRGIIVGRGWTQVGGRPHAEVVALNNAGYLAKGATVYVTLEPCSHYGKTGPCALKLIDAGIKRMVIAARDPDVRVSGNGIKLLRDANIDVKCDLMYKEAQELNVGFFFTKIKGRPFITVKLASTLDGKIALSNGNSKWITNQLTRNWVHKQRSMYDAVMVGSNTVVHDDPMLNTRIPGLECYSPVRIVIDRFGKICTYHNIVKTADIIPTYILTDDTSKTKLGKAKYLIVTGTDNFLKNAMKVISMKIGITRLFVEGGSILITELLKSQLVDRIIWCRSNKIFGANAVSSVGDLNILTLQNLYNFRKIDTLYFDDDVVDILENCRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSZ6</accession>
    <name>Q3YSZ6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MerR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0108"/>
    <dbReference type="EnsemblBacteria" id="AAZ68159">
        <property type="protein sequence ID" value="AAZ68159"/>
        <property type="gene ID" value="Ecaj_0108"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0108"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045945_4_2_5"/>
    <dbReference type="OMA" id="LRFWEEK"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="15"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="124" mass="14477" checksum="5CC8E5A47B6F13C6" modified="2005-09-27" version="1">MSGKKLSQSSSKLQTIGEVAKCLNVEQYVLRFWEEKFPQINPIKRRGRRLYTQVDVDILKYIKHLLYDKGYKIKGVQQELSKKPVNIDKSNASVKQNVYREGLISLLDDMLNLRNDLLKKFDGM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT16</accession>
    <name>SYV_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Valine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Valyl-tRNA synthetase</fullName>
            <shortName evidence="1">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">valS</name>
        <name type="ordered locus">Ecaj_0088</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304217.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT16"/>
    <dbReference type="SMR" id="Q3YT16"/>
    <dbReference type="STRING" id="269484.Ecaj_0088"/>
    <dbReference type="EnsemblBacteria" id="AAZ68139">
        <property type="protein sequence ID" value="AAZ68139"/>
        <property type="gene ID" value="Ecaj_0088"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0088"/>
    <dbReference type="eggNOG" id="COG0525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_5"/>
    <dbReference type="OMA" id="RQWYIRN"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02005">
        <property type="entry name" value="Val_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022874">
        <property type="entry name" value="Valine-tRNA_ligase_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="PTHR11946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224608" description="Valine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="524"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="527"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02005"/>
        </source>
    </evidence>
    <sequence length="802" mass="93049" checksum="4B1A79DFF931A674" modified="2005-09-27" version="1">MQSIFNNKYQFKNIETKYNTLWDTTKLYKWKNSGTNQFVIDTPPPTISGQLHIGHVFSYCHTDFIARYQRMLGKDVFYPIGFDDNGLPTERLVEKTKKIRATDISRKEFKTICTQVSHEFRIQFKQLFQSIGISYDWDLEYHTISKDIQKISQTSFINLYNKGKLYRKLQPIFWDCIDKTAIARAEVEENELSSFMNTIAFSTEAGKAINIATTRPELMPACVAVFFNPSDIRYQDLLGQNAIVPIFGNKVKILSDDQVKIDKGTGLVMCCTFGDEMDVYWWNKHNLDTKIIISKSGTIDHLNTLQGQDVCKQLHGLSITEARALIIEILERNNLLIQKQEITHNVKCAERSGAPIEILLSHQWFIKVVDIKHELLKQVQKINWHPQSMRKQIEIWIEGLNWDWCISRQRYFGVPFPVWYSKDGKIILPDINKLPIDPTNDLPEGYQDTEIEVETDVMDTWATSSLSTQFHNISATPADLRAQSHEIIRSWAFYTILQAYYHNNDIPWKNIMISGWCLAEDKTKMSKSKGNVLTPNKLLDEYGADVVRYWTANSKLGADTTFSNEILKLGKRFTTKLWNASKFVSMFIDQYSEPDLQYITETMDKWILSKLYKVIVKATESFNSFEYCIALDCIESFFWKDFCDNYLELSKKRAYGELISKQEHLSAVNTLSFVLRELLKMLAPFMPYVTEEIYRTLYSSNNSIHSHNTWPAADVNLYNESDELLGETFIEILNQVRKVKASAQLSVKYKINKLIINKHFPVSLENDLKAVCNADCIVYDNRQNDNKEQLLVSVEFENVQIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT24</accession>
    <name>Q3YT24_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative Uncharacterized membrane-bound protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304209.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0080"/>
    <dbReference type="EnsemblBacteria" id="AAZ68131">
        <property type="protein sequence ID" value="AAZ68131"/>
        <property type="gene ID" value="Ecaj_0080"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0080"/>
    <dbReference type="eggNOG" id="COG3898">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055260_0_0_5"/>
    <dbReference type="OMA" id="LLYWTDK"/>
    <dbReference type="OrthoDB" id="1425742at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010817">
        <property type="entry name" value="HemY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07219">
        <property type="entry name" value="HemY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="38"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="HemY_N" evidence="5">
        <location>
            <begin position="24"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="413" mass="48820" checksum="83D30A6DB1900A46" modified="2005-09-27" version="1">MITSLLIFVIVALTIGLWVIDCDGIITIDWLGYDIEVNILFTFFVVAVFLLVLILLVRLVFCLIQSLYRYRRDSQNKKMILLEQGYMYLNCGDVERVEKIITKLGSFDHPSFYLLKGRVCFDNRKYGLAEKYFGQFAKIVPMIDASLGVHLLNVISQEEDQIQQLSLLRKMLEIFYKQSWSAIFKLEIYRLSRDWSNAIEEIKKVIKLKVNLPYNVQDMLNIFYYALAKQCYDVQRYDDGLKVLDNIKNCQKYSTPIVLLKAQCYLNTDRKRKAVSILEYEYRTNPHPDIANLYLDIMQHNSHAIHKLYSFNTEYYFSIYLIARDAINLGEYDAAMKYLNNEFKTQTYISLYFLMLKLKVLSQDYNELLYWTDKIAKDAISEQHWSCTKCQCVSTRWHYECDNCKGFNTIVWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT38</accession>
    <name>Q3YT38_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304195.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68117">
        <property type="protein sequence ID" value="AAZ68117"/>
        <property type="gene ID" value="Ecaj_0066"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0066"/>
    <dbReference type="HOGENOM" id="CLU_324591_0_0_5"/>
    <dbReference type="OrthoDB" id="2107298at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021902">
        <property type="entry name" value="DUF3514"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12027">
        <property type="entry name" value="DUF3514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012022889" evidence="2">
        <location>
            <begin position="16"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="889" mass="99211" checksum="4A18902A3A952B21" modified="2005-09-27" version="1">MLFLAVISIVGSAAAAVLLRLIINAAQNEKNEEEEQKESDKERCVQESGVDSHSNGKVRDKSTMSDKNVRGGKKKRRSRSNKKGIQEIDKTDQDKQSNAGAASSEGMKEKKCELEKKDIRIPTDIETGKEKGAFGLQSTSDSVLSTDLQTQYLALGARLKERTKSVKSKKSKPTKKSDVQVAMAVDSDQVKKDDKSSDRSVSTGGKDKEKLEKKRQPESAREVIVQSTDRVTADKVSQKQDEERSTETSSSKDVKSTEGSKRGLEQKVPAKDDESDEGVQNKNKEHDTKPNEQVDQSADKDLQHQPGGGPIRSASEHSGTPSQSKDTSAISSDVSQASDGQDKYLALGARPKVRTSKPSKSKMPKSAKKPAVQFFDWDSKYYKVQDPVLVQAGKEYNADIKSTSSILPADEESISTLLYGTKVQQKHSESTDAGLSFNVLIDKTSTQVNEDLVGKGQDFLTIQSMRLKESSLDAQEMTILKSAGQRVSDTVISPVETPDIVQAVCDQIIQKDECEETADSTQLQCLKSVKISKVDVRGRQESVIAFDQSVLPSAFEILALASKKSQAKRQALNIKFYNFKMMHLQYCYDIAQEMFFVQNNKLYTKYDLLSFLRLNIQNYELCVFLLKLSYVLNCTIVIRQPTFAKNLVYNASFFHEPSILCDCIGKLFSSVQSGYTSIEVMHKILCSYHVKRFPQNSTLYSGAFFSELLKILGHLVSIRLSEPNYLNKILDFAIVNCSMMLGSMYSNYRYLTGIKGMIADEATFNMLVDHMGSTGSCKGKLGLMCYRVLCNILYMYNGDYNNDVLICGMYLPERVVKQCSQYFSDKIVNNVVNNTMGFHVIMEDISMLICSMIDRFNLAAFEKEIQAYSNLYDMMVRSSGTQKDQGHGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT43</accession>
    <name>Q3YT43_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="4">2.3.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + acetyl-CoA = (R)-N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:17017"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="2.3.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="4">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.12" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304190.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0061"/>
    <dbReference type="EnsemblBacteria" id="AAZ68112">
        <property type="protein sequence ID" value="AAZ68112"/>
        <property type="gene ID" value="Ecaj_0061"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0061"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_10_2_5"/>
    <dbReference type="OMA" id="IPMVTHF"/>
    <dbReference type="OrthoDB" id="1626282at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043231">
        <property type="term" value="C:intracellular membrane-bounded organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:acetyl-CoA biosynthetic process from pyruvate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006257">
        <property type="entry name" value="LAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="PTHR23151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01349">
        <property type="entry name" value="PDHac_trf_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="6">
        <location>
            <begin position="130"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="403" mass="44387" checksum="F115E25D13DA1A35" modified="2005-09-27" version="1">MPIEILMPALSPTMKNGTIRKWYKSEGDIIKSGDIIADIETDKAVMEFEYTDEDGIIGKIFFAEGSKDIAVNQLIALIAVDEHDLVNVQSYKKRDDVSQNNSNALQVNQQVVSSNEEVLVNQSNVSERIKISPLAKKIAADLCVDINLIKGTGPYGRIIKADVLDAASQKKEHVSSSPMSFTEISSMRRVIAERLVYSKQSIPHFYVSIDCIVDDLLKLRLEINAENSDTKVTVNDFIIKAVAMSIKKFPEINVSWSDDKIVVFHNVDISVAVSIDSGLITPIIFNADKKSLLEISSEVKTLASKAKSGKLRSEEFQGGGFTISNLGMFGIKEFCAIVNPPQSCIMAVGCSEKRAIVVDDQISISNVITITLSVDHRVIDGVLAAKFLSCFKSYLEKPFLMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT48</accession>
    <name>Q3YT48_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">5-aminolevulinate synthase</fullName>
            <ecNumber evidence="6">2.3.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">5-aminolevulinic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Delta-ALA synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Delta-aminolevulinate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ecaj_0056</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycine + H(+) + succinyl-CoA = 5-aminolevulinate + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="2.3.1.37"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.37" evidence="6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304185.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0056"/>
    <dbReference type="EnsemblBacteria" id="AAZ68107">
        <property type="protein sequence ID" value="AAZ68107"/>
        <property type="gene ID" value="Ecaj_0056"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0056"/>
    <dbReference type="eggNOG" id="COG0156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_11_1_5"/>
    <dbReference type="OMA" id="NHASMIV"/>
    <dbReference type="OrthoDB" id="479874at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00375"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003870">
        <property type="term" value="F:5-aminolevulinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010961">
        <property type="entry name" value="4pyrrol_synth_NH2levulA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01821">
        <property type="entry name" value="5aminolev_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="14">
        <location>
            <begin position="46"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAZ68107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="398" mass="44140" checksum="5604687BC1F5DE23" modified="2005-09-27" version="1">MTNYEEIFDNKIKRIKEEGRYREFTGFSRIPGQFPYAIECNVNNVVTLWCSNDYLGMGQSEHVMLAIKNYSSSVGAGGTRNISGTTKEIIELEESLADLHKKPAALTFVCGYVANQTTISTLLSVIPDIVVFSDEKNHSSMIEGIRGSNRAKHIFKHNDVKHLEALLKSVDRSLPKIIIFESLYSMDGDIAPIAEICDLADEYGAMTYLDEVHAVGMYGNHGGGISEQENISDRITIIQGTLSKAFGVMGGYITGSKSLIDVVRSFAPGFIFTTALSPLIASSARISVEHLKNSSVEREKQREVVNKVKESFSRTGINFLKTDTHIIPIIIGDPVACKDISKILLNDYKIYIQSINYPTVPVGTERLRITPTPYHTDEMIDILTQALVDVLGRFKIVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT53</accession>
    <name>Q3YT53_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of an ABC transporter complex. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304180.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0051"/>
    <dbReference type="EnsemblBacteria" id="AAZ68102">
        <property type="protein sequence ID" value="AAZ68102"/>
        <property type="gene ID" value="Ecaj_0051"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0051"/>
    <dbReference type="eggNOG" id="COG1127">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_5"/>
    <dbReference type="OMA" id="PKYLFCD"/>
    <dbReference type="OrthoDB" id="1058434at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030296">
        <property type="entry name" value="MlaF/Mkl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43023:SF6">
        <property type="entry name" value="PTHR43023:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="238" mass="26202" checksum="494B492457A112AF" modified="2005-09-27" version="1">MHYISLSDLHIDFRGHQVLKGISLDIAYGESLVIVGESGSGKSVLTKTILGLIAPTSGKVVISGVDIRHDKSLIKNFGVLFQNCALFDSLTVCENIAFNLHYRFNRDRSLSRKIAASGLNLVGLGDDVLDRYPIELSGGMKKRIALARAIVSEPKIMILDEPTSGLDPIMSDVVNEIIIKCHKEFNLTVITITHDISSAFKIASKIAVLYNGKIIACESVQDIRNIDNQYVKNFICRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT55</accession>
    <name>Q3YT55_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10 11">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7 10 11">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpe</name>
        <name evidence="15" type="ordered locus">Ecaj_0049</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10 13">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 10 11">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7 10 11"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0049"/>
    <dbReference type="EnsemblBacteria" id="AAZ68100">
        <property type="protein sequence ID" value="AAZ68100"/>
        <property type="gene ID" value="Ecaj_0049"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0049"/>
    <dbReference type="eggNOG" id="COG0036">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054856_2_1_5"/>
    <dbReference type="OMA" id="WLQVDGG"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00326"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="PTHR11749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="147"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="202"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10 12">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 12">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13 14">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAZ68100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="222" mass="24420" checksum="46D57E93FF0A36F5" modified="2005-09-27" version="1">MLKKKRIVEVSSSILSADFANLSKSIEVLENASVDYIHIDVMDGNFVPNITIGPVVVSAIRECTKIPFDVHLMINSPSNYIETFVNAGADMITVHAESEVHLDRVIKKIKSYGKDAGLSLVPTSHYSILEYIIFELDLVLIMTVNPGFSGQQFIVSQLDKISRVRSMIEKYSLKTKIAVDGGVNIDNAKSVIEAGADILVVGSAIFKAKNVLDYVQKLKSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT71</accession>
    <name>Q3YT71_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphopantothenoyl cysteine decarboxylase</fullName>
            <ecNumber evidence="2">4.1.1.36</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.1.36" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304162.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0033"/>
    <dbReference type="EnsemblBacteria" id="AAZ68084">
        <property type="protein sequence ID" value="AAZ68084"/>
        <property type="gene ID" value="Ecaj_0033"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0033"/>
    <dbReference type="eggNOG" id="COG0452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033319_2_0_5"/>
    <dbReference type="OMA" id="NTRMYDH"/>
    <dbReference type="OrthoDB" id="1429052at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="1">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="177" mass="19406" checksum="40F4789FECDBFA47" modified="2005-09-27" version="1">MKILLVISGSIAAYKSLDLIRKLQENNHTVTGIISKSGEKFVTQLSVASLSGNYAYTDADFFNIHNSMHHISLTRNSDILLVAPATLDIIAKTAHGIADELATTVLIASNIPIIMAPAMNPVMWYSKANQRNIKCLRNDNVVIIEPKEGLAVCNEYGLGKMAETQDIVSFIDNFQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT78</accession>
    <name>Q3YT78_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Diaminopimelate epimerase</fullName>
            <shortName evidence="8">DAP epimerase</shortName>
            <ecNumber evidence="4 8">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dapF</name>
        <name evidence="10" type="ordered locus">Ecaj_0026</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="4 8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304155.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0026"/>
    <dbReference type="EnsemblBacteria" id="AAZ68077">
        <property type="protein sequence ID" value="AAZ68077"/>
        <property type="gene ID" value="Ecaj_0026"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0026"/>
    <dbReference type="eggNOG" id="COG0253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053306_1_0_5"/>
    <dbReference type="OMA" id="HVAMRVH"/>
    <dbReference type="OrthoDB" id="1921631at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="PTHR31689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="71"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="202"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="212"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="267" mass="29686" checksum="E8F40E585C62FAA1" modified="2005-09-27" version="1">MIDFIKMHGTLNDFVIIDCRSKFYNNIDYKAIANRKTGIGCDQIIVITKSEKADCFMHIYNADGSKVEMCGNAARCVAYLLSNEKQKNNVTIELADRILSCLRISENTVQVNMGIAKFHWTDIPISQECDTLHLPITLEMLSDPVGINVGNPHAIFFVNSIETIPLDKLGPKLENHNLFPKRANISIAEILSRNKIKLRVWERGTGETASCGSGACAALVAAVRRQYTDTTATICLQGGNLLISYQNDNTILMEGMVSYTFHGIYCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT86</accession>
    <name>Q3YT86_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TRAG protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0018</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VirD4/TraG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0018"/>
    <dbReference type="EnsemblBacteria" id="AAZ68069">
        <property type="protein sequence ID" value="AAZ68069"/>
        <property type="gene ID" value="Ecaj_0018"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0018"/>
    <dbReference type="eggNOG" id="COG3505">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012039_2_1_5"/>
    <dbReference type="OMA" id="FIYEPFS"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003688">
        <property type="entry name" value="TraG/VirD4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02534">
        <property type="entry name" value="T4SS-DNA_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="567"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="636"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="576"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="593"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="636"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="723" mass="83636" checksum="824DBCEBFF406610" modified="2005-09-27" version="1">MDSISANHIRNILFLVLGAFFGLEFCFYLSGVLFILMVWGPNYLDFNAINPSLSDFPDRIWPTIFDYVQHWWKNPSAYDAVLLLKLITSLCTPVGILSIVLWNLRNILFDWRPFKKKESLHGDSRWATEKDIRKIGLRSRKGILLGKDKRGYLIADGYQHALLFAPTGSGKGVGFVIPNLLFWEDSVVVHDIKLENYDLTSGWRKKRGQEVFVWNPAQPDGISHCYNPLDWISSKPGQMVDDVQKIANLIMPEQDFWYNEARSLFVGVVLYLLAVPEKVKSFGEVVRTMRSDDVVYNLAVVLDTIGKKIHPVAYMNIAAFLQKADKERSGVVSTMNSSLELWANPLIDTATASSDFNIQEFKRKKVTVYVGLTPDNLTRLRPLMQVFYQQATEFLCRTLPSDDEPYGVLFLMDEFPTLGKMEQFQTGIAYFRGYRVRLFLIIQDTEQLKGIYEEAGMNSFLSNSTYRITFAANNIETANLISQLIGNKTVNQESLNRPKFLDLNPASRSLHISETQRALLLPQEVIMLPRDEQILLIESTYPIKSKKIKYYEDKNFTKKLLKSTFVPTQEPYDPNKTKTATKENEEPMPSIESDLPKNTSDNTENNMEDGAMYSSIEEDYDDDDDDFNFEDLDEYMDEEEDYDDEEYDDIDYDDNNNSNEEYEEDNPEEDDNSNNLDDEEEEEDNIIDYEDEEEYDDNIDYKDDDNNYNKDTTDDQDSKKHNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQN3</accession>
    <name>Q3YQN3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="2">P5C reductase</shortName>
            <shortName evidence="2">P5CR</shortName>
            <ecNumber evidence="2">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">proC</name>
        <name evidence="5" type="ordered locus">Ecaj_0941</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-proline + NADP(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305045.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0941"/>
    <dbReference type="EnsemblBacteria" id="AAZ68972">
        <property type="protein sequence ID" value="AAZ68972"/>
        <property type="gene ID" value="Ecaj_0941"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0941"/>
    <dbReference type="eggNOG" id="COG0345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_0_0_5"/>
    <dbReference type="OMA" id="VVRVMTN"/>
    <dbReference type="OrthoDB" id="1349288at2"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PTHR11645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="3">
        <location>
            <begin position="3"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="P5CR_dimer" evidence="4">
        <location>
            <begin position="159"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="268" mass="29662" checksum="BF329A4EB070CD2D" modified="2005-09-27" version="1">MNILLIGCGNLGSILLNKWSHNAQISKVIVVQPSLSKRDAFNSITKVTFVKDYEKISRDFVPQVVVIAIKPQQIFDVMPNYGIYSKSASFISLCAGIDVNLLKKCLGNTATIIRVMPNIAMSLSKSVNLSYMEEESHNEKQKVSIIFNDTGLIYWIQKEDIFDKLSPITGSGPAFFFSLAEELVKHTVEAGINEEDAIKLVSQTFIGSAKLLETRKTTISNIISSITSRGGITQAALQVLNLDLPKTISSCLKAAMERLLYLKDMHNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQN4</accession>
    <name>Q3YQN4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ThiJ/PfpI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305044.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68971">
        <property type="protein sequence ID" value="AAZ68971"/>
        <property type="gene ID" value="Ecaj_0940"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0940"/>
    <dbReference type="eggNOG" id="COG3155">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072952_1_0_5"/>
    <dbReference type="OMA" id="AQVQCFA"/>
    <dbReference type="OrthoDB" id="1421548at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026041">
        <property type="entry name" value="ElbB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10224:SF12">
        <property type="entry name" value="PTHR10224:SF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="219" mass="24282" checksum="B88977FD0EC5EADB" modified="2005-09-27" version="1">MKLNSAVILSGCGHMDGSEIREAVLVMLELDRNNVSFKCFAPNKNQKQVIDHQKKESVEEVRNILVESARIARGSIYDIEQINVEEFDMLVIPGGYGVAKNFSNLFDEDKQGESILPEFKNAVCKFYNAKKPIGAVCISPAVVVEVLKDIAKVKVTIGEDSNNLIDKLGGIHVDCPTIKSVQDDVNKIFSCSAYMRNDSLYNVYLGIQDMISSMVNSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQP1</accession>
    <name>Q3YQP1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Competence/damage-inducible protein cinA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305037.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0933"/>
    <dbReference type="EnsemblBacteria" id="AAZ68964">
        <property type="protein sequence ID" value="AAZ68964"/>
        <property type="gene ID" value="Ecaj_0933"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0933"/>
    <dbReference type="eggNOG" id="COG1546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_1_2_5"/>
    <dbReference type="OMA" id="VEHALNM"/>
    <dbReference type="OrthoDB" id="1834406at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="SSF142433"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00199">
        <property type="entry name" value="PncC_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CinA" evidence="1">
        <location>
            <begin position="11"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="160" mass="17267" checksum="9404D797AFF25575" modified="2005-09-27" version="1">MIIKQEILEKAKTCVLKLVENNSKIVIAESCTAGLMSFLLSCIPGASKVLDCSFVVYSNEAKANILDIDKELIDKCGAVSSEVSILMSVGALKNSRANVSISITGFAGPDGEQVGLVYVGYASNVDSDYKKCCFSDNMSRNEIQMLSVNAAMDFLLYKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQP9</accession>
    <name>Q3YQP9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ftsZ</name>
        <name evidence="8" type="ordered locus">Ecaj_0925</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305029.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0925"/>
    <dbReference type="EnsemblBacteria" id="AAZ68956">
        <property type="protein sequence ID" value="AAZ68956"/>
        <property type="gene ID" value="Ecaj_0925"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0925"/>
    <dbReference type="eggNOG" id="COG0206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_5"/>
    <dbReference type="OMA" id="IMNQGGV"/>
    <dbReference type="OrthoDB" id="1009041at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 5 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="6">
        <location>
            <begin position="16"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="7">
        <location>
            <begin position="210"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="24"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="420" mass="45513" checksum="6E493043F7028D61" modified="2005-09-27" version="1">MSLNICLPDQSLLRPRITVFGVGGAGGNAVNNMIQSNLHGVNFVVANTDAQALELSLSEKKIQLGIGLTKGLGAGSLPEVGRGAAEESINEIIEEISDSNMLFITAGMGGGTGTGAAPVIAKVAKENKILTIGVVTKPFHFEGAHRMRTAELGLEELQRYVDTLIVIPNQNLFRIANDKTTFADAFKLADTVLHTGVRGITDLMVMPGLINLDFADIRAIMSEMGKAMMGTGEAEGENRAILAAEAAISNPLLDNISMKGAKGILINITGGLDMTLFEVDAAANRIREEVDSHANIIFGSTFNKESEGKIRVSVLATGIDNEEVVIQNKSTLKNKETQDNKLSEISNKSFNPSDNEIAYYKPSDPGQDMINSINHIRKQDNIENQVSNNKIYESAYKAQFNKNEFWDEESLNIPTFLRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQQ2</accession>
    <name>Q3YQQ2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0922</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305026.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68953">
        <property type="protein sequence ID" value="AAZ68953"/>
        <property type="gene ID" value="Ecaj_0922"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0922"/>
    <dbReference type="HOGENOM" id="CLU_1903385_0_0_5"/>
    <dbReference type="OrthoDB" id="2123760at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="133" mass="14990" checksum="6434D3679C4EE9F0" modified="2005-09-27" version="1">MSIDYDSCQIDFSIQRSGHPGVDVYHGIMHVNEEGSSYYASGHIVNDSGNEGIVNIIKYCNRGIYNDEHCTECDLEVILDDPNSSRYVFHVSKDDTCLMCDERNILSMMKPSRKVQIQNDAVVALDIYGCKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQR4</accession>
    <name>Q3YQR4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305017.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0910"/>
    <dbReference type="EnsemblBacteria" id="AAZ68941">
        <property type="protein sequence ID" value="AAZ68941"/>
        <property type="gene ID" value="Ecaj_0910"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0910"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="ISPYICA"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230983" evidence="1">
        <location>
            <begin position="29"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="293" mass="32233" checksum="0A15E94B1D2A3B65" modified="2005-09-27" version="1">MNNKLKFTIINTVLVCLLSLPNISSSKAINNNAKKYYGLYISGQYKPSVSVFSNFSVKETNVITKNLIALKKDVDSIETKTDASVGISNPSNFTIPYTAVFQDNSVNFNGTIGYTFAEGTRVEIEGSYEEFDVKNPGGYTLSDAYRYFALAREMKGNSFTPKEKVSNSIFHTVMRNDGLSIISVIVNVCYDFSLNNLSISPYICGGAGVDAIEFFDVLHIKFAYQSKLGIAYSLPSNISLFASLYYHKVMGNQFKNLNVQHVAELASIPKITSAVATLNIGYFGGEIGARLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQR5</accession>
    <name>Q3YQR5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0909</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0909"/>
    <dbReference type="EnsemblBacteria" id="AAZ68940">
        <property type="protein sequence ID" value="AAZ68940"/>
        <property type="gene ID" value="Ecaj_0909"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0909"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="RIEMGFS"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="294" mass="32869" checksum="570FAC76A5820867" modified="2005-09-27" version="1">MANFMYKKYKLMTAGVVLFHMLFLPHVSFAKNTNSNKLGLYISGQYNPSVSVFSNFSAKETNVHTVQLMALKKDIDSIEVDTGNSAGISKPQNFTVLYTPKFQDNVAGLSGALGFFYSKGLRIEMGFSYEKFDAKDLGEYTKIKDAYRYFALVREMHVSLIYPKDNNTGTHYTVMRNDGISISSATVNGCYDFFFPSLSLSPYMCIGIGIDAIEFLNALHIKFACQGKLGVTYSVSPNVNLFADGYYHKVMGNKFKNLPVQYVNTLEEYPRVTSAIATLDIGYLGGEIGIRFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQU0</accession>
    <name>Q3YQU0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein translocase subunit SecD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">secD</name>
        <name evidence="11" type="ordered locus">Ecaj_0883</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a complex with SecF. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SecD/SecF family. SecD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0883"/>
    <dbReference type="EnsemblBacteria" id="AAZ68915">
        <property type="protein sequence ID" value="AAZ68915"/>
        <property type="gene ID" value="Ecaj_0883"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0883"/>
    <dbReference type="eggNOG" id="COG0342">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007894_4_3_5"/>
    <dbReference type="OMA" id="SAHLQMM"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01463_B">
        <property type="entry name" value="SecD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005791">
        <property type="entry name" value="SecD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00916">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
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        <location>
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            <end position="364"/>
        </location>
    </feature>
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        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
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            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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            <end position="464"/>
        </location>
    </feature>
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        <location>
            <begin position="470"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="508" mass="56210" checksum="2DEC7EE38E1FC924" modified="2005-09-27" version="1">MDDMFSKLQIKPIIVSLLCILAIYLILPNFIDSKFLLSKQKVNLGLDLQGGVYLLLEADFKEYLKERMYALYDELNEFLLSKKIEYSRFDVEQDKLVLTLKDGKSFDKVKLFGNKNVRISEREGCVEISFYDSYKSELLRNVISDAISNIRRRLDKSGTKEIIINSHGKDRISLQVPGVHDTSQIKTLLGKTAKLTFHLLEDVKQLSSINSLTTVLLKDNRGNTYPILKKVEISGDSLIDVSSGINSLGNVIVHFKLNNEAAKKFAKITKENFNKPFAIVLDGVVLTAPIIREPILTGSGEISGNFTVETAKELSILLKSGALPVPLKVIEEKTIGSSLGAEYIKQGRLAMIISVIAVSVFIIFSYRMFGVLAVVGLVFNIIFIVAVLTLLQATLTLPGLAGITLTVGMSVDANVLIFERIREELNSTKKLRWAVESGFKNAMSTIFDSNITTLIVAAVMFVIGSGPISGFAITLSIGILCSMFSAITLTKMLIDLYIKLFNLKTLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQU2</accession>
    <name>Q3YQU2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
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            <taxon>Anaplasmataceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68913">
        <property type="protein sequence ID" value="AAZ68913"/>
        <property type="gene ID" value="Ecaj_0882"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0882"/>
    <dbReference type="HOGENOM" id="CLU_516530_0_0_5"/>
    <dbReference type="OMA" id="DIGISEC"/>
    <dbReference type="OrthoDB" id="596002at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="393"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="527" mass="59470" checksum="3DBE03F1421F0A08" modified="2005-09-27" version="1">MPNNNDYDTYSKFIRDIRSTVFYIRNRGVLHLGDLVSNVTPATYIIDSAKEGIREQFLSRVFVHHSNNKNDIKKVIDFIISLGFIPVVNADYKVTEHEGVCIYDKNRKYGDKHVLIVSSVVRHTTRDVRNIILESRKNFRSSFDDGAKDSIRTVLYDPDSKPLKQRIYDAISYSEKWSLLTTQEKVIRCIFTVLSLSIPLIPPLLLLSYIGNIDIREAAYSMNLLPWQYRVYMKTSDSLYDVIVNNSDEERRKMITDNIMETAMAKGFVVKGEGDEIQPTDLGKHVSGKHNLLLVAHNKELSKKQLLLRITTFSVLAFVCFSQIIALTCHLVRAEKIALYCSILSYFVIFVEGLYLLSLKTTMSKVCATFCLVFAGFMLMVHAFLLYNDITFGYGPIMSGLMVIITGAVLSVALACNQYYKQDRDLALKLGGISTTAYLSMKRDIDGIDEVKTVKLEGGCGYQIIDDLSLALFNAYNAGVFGTVQDQTVGNRQDEIYSGTQENDNCPNTSIGDLQSIDRSNSQDLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQV7</accession>
    <name>Q3YQV7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="13">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="13">2.7.8.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ecaj_0867</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="10">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="13"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0867"/>
    <dbReference type="EnsemblBacteria" id="AAZ68898">
        <property type="protein sequence ID" value="AAZ68898"/>
        <property type="gene ID" value="Ecaj_0867"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0867"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051314_2_1_5"/>
    <dbReference type="OMA" id="WSMVYYL"/>
    <dbReference type="UniPathway" id="UPA00664"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004570">
        <property type="entry name" value="Phosphatidylglycerol_P_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000847">
        <property type="entry name" value="Phos_ph_gly_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00560">
        <property type="entry name" value="pgsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="185" mass="20902" checksum="C8BEA06C8FA31BE8" modified="2005-09-27" version="1">MVDDFRRTLPNLLTILRVFAIPAIVCSFYIGDNNSNYIAFIIFIFACVTDFFDGYLARIWKTQSKFGKLFDPIADKLIVAATVIMLIYIGKITGITIIPVVIIICREILISGLREFLISMNVELPVIKLGKIKTFIQMAAIAMLMLNDSLILYIGEVILYFAAALTIYSAYLYVCIAIKYICLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQW6</accession>
    <name>Q3YQW6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transferase hexapeptide repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0858</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304966.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0858"/>
    <dbReference type="EnsemblBacteria" id="AAZ68889">
        <property type="protein sequence ID" value="AAZ68889"/>
        <property type="gene ID" value="Ecaj_0858"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0858"/>
    <dbReference type="eggNOG" id="COG0663">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064827_7_1_5"/>
    <dbReference type="OMA" id="YVTIGHN"/>
    <dbReference type="OrthoDB" id="1242595at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="172" mass="18944" checksum="F7FB61C3A4D06B10" modified="2005-09-27" version="1">MHNIISYGIFVPNIDGTVFVASTASIVGSVYISKNASIWYNSVLRGDVGMISIGEGTNIQDNTVIHVDRNQGDTEVGKMVTIGHGCILHACQIHDYVFVGMGSIIMDKVIMEENTMLAAGSLVTKGKVIKSGELWAGRPAKFFRMLSEEELNHIKESADNYIRLSQEYLECR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQY2</accession>
    <name>Q3YQY2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0842</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304950.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68873">
        <property type="protein sequence ID" value="AAZ68873"/>
        <property type="gene ID" value="Ecaj_0842"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0842"/>
    <dbReference type="HOGENOM" id="CLU_148745_1_0_5"/>
    <dbReference type="OMA" id="SKFDCSQ"/>
    <dbReference type="OrthoDB" id="1910088at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007039">
        <property type="entry name" value="TrbC/VirB2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04956">
        <property type="entry name" value="TrbC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="125" mass="13276" checksum="D11D3355DFB436CB" modified="2005-09-27" version="1">MVYSVSKYLLFFIFIMFFMLPTNNAFSSSAVQGTTDDDTVTKVICNVVVFVQKLGLPIMTGVILGSSIMAIFGRLPWPAIVMLVVFTAIFFGAGKLISKFAGGIDSVKDGGKFDCGKVAGTTAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR06</accession>
    <name>Q3YR06_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0818</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304926.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0818"/>
    <dbReference type="EnsemblBacteria" id="AAZ68849">
        <property type="protein sequence ID" value="AAZ68849"/>
        <property type="gene ID" value="Ecaj_0818"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0818"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2932">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_1_2_5"/>
    <dbReference type="OMA" id="CEEHISE"/>
    <dbReference type="OrthoDB" id="1933795at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06529">
        <property type="entry name" value="S24_LexA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039418">
        <property type="entry name" value="LexA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019756">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00501">
        <property type="entry name" value="SPASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="211" mass="23816" checksum="F3FACB483D125ABA" modified="2005-09-27" version="1">MKTSSDNSMIVTRMKYQMDKMGINARELAHKADVGKSFVYDILSGKSTNPTSKKLMAIAKVLNVSLSYLISDDNYTYGQGNMNILPVYDLELENGKISSSGDVNLYLSSNINLTSNMKNLRVYYVKGDSMIPTLMNQDVVLVDIGDKIPHPAGLFVIVDTVGVSIRRLEYLRENQKIKLHVVSDNKKYSSYECHLEDMEILGRVIWYARSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR13</accession>
    <name>COXX_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protoheme IX farnesyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heme B farnesyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ctaB</name>
        <name type="ordered locus">Ecaj_0811</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E,6E)-farnesyl diphosphate + H2O + heme b = diphosphate + Fe(II)-heme o</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.141" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304919.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YR13"/>
    <dbReference type="SMR" id="Q3YR13"/>
    <dbReference type="STRING" id="269484.Ecaj_0811"/>
    <dbReference type="EnsemblBacteria" id="AAZ68842">
        <property type="protein sequence ID" value="AAZ68842"/>
        <property type="gene ID" value="Ecaj_0811"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0811"/>
    <dbReference type="eggNOG" id="COG0109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029631_0_2_5"/>
    <dbReference type="OMA" id="QFFWQFP"/>
    <dbReference type="OrthoDB" id="1875497at2"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PTHR43448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000327048" description="Protoheme IX farnesyltransferase">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00154"/>
        </source>
    </evidence>
    <sequence length="295" mass="32845" checksum="F79F60A82391A51B" modified="2005-09-27" version="1">MNSKCETIKPQVVNSILDYWNLLKPKIMYLVVLTGITGMIIAPGSIHPFIAIISTLCIALGSGAAGAINMWYDSDIDALMTRTKNRPIPAGKISKSSAIELGLVLSVISVTVMMISVNYISGILLAISIGFYSFAYTMYLKRRTPQNIVIGGIAGAIPPIIGWTSVTSSISIESLILFLIIFMWTPPHFWALSLLNYQEYEKAKIPMLPVTHGIFVTKVHILVYSIVLFIITLLPGLFLKDYLLYEICAIPLGITFLFHAFKVFTSSNYYKYKAMFTYSVAYLFILFICIILASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR19</accession>
    <name>Q3YR19_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heat shock protein HslU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0805</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpX chaperone family. HslU subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304913.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0805"/>
    <dbReference type="EnsemblBacteria" id="AAZ68836">
        <property type="protein sequence ID" value="AAZ68836"/>
        <property type="gene ID" value="Ecaj_0805"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0805"/>
    <dbReference type="eggNOG" id="COG1220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033123_0_0_5"/>
    <dbReference type="OMA" id="KYGMIKT"/>
    <dbReference type="OrthoDB" id="718259at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009376">
        <property type="term" value="C:HslUV protease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004491">
        <property type="entry name" value="HslU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102:SF3">
        <property type="entry name" value="PTHR48102:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00390">
        <property type="entry name" value="hslU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="105"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="ClpB_D2-small" evidence="7">
        <location>
            <begin position="384"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="492" mass="55293" checksum="8D61C1E16E2AD06F" modified="2005-09-27" version="1">MFVKLKLNDDLESNTTTTSDNTHDNQDHNQTCATKSKNIISVTATEDETTSDDSYNTQELTPQQITQALDRFIIGQADAKRAVAIALRNRWRRNRVPEPLREEIIPKNILMIGHTGIGKTEIARRLAKLAKAPFIKVEATKFTEIGYVGRDVDSIIRDLVDVAINLVKEKFRKIVEKKAKALSESMILDALIGPDASEETKTIFQEKLRNGEFEDSEISISIKESKNTMPPIDIPNIPGNQVGIMNINEIVHKMLGNNKQLKTIKVTVKEARELLINEESEKLMDEDKIIKEALQLASNDGIVFLDEIDKIAARTEIRGEVNREGVQRDLLPLLEGTSVTTKYGTITTDHILFIASGAFHLAKPSDLLPELQGRLPIRVELKPLSKDDLVRILTEPESSLLKQYCALMKTENITIDFTDEGVCTIAEIASTVNREVENIGARRLHTILEKLMEDISYTATENSGKTYVIDSEYVKQKLEDISKQLDLSKFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR28</accession>
    <name>Q3YR28_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0796</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304904.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68827">
        <property type="protein sequence ID" value="AAZ68827"/>
        <property type="gene ID" value="Ecaj_0796"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0796"/>
    <dbReference type="HOGENOM" id="CLU_155693_0_0_5"/>
    <dbReference type="OMA" id="SFIIYKH"/>
    <dbReference type="OrthoDB" id="2093395at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024399">
        <property type="entry name" value="DUF2628"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10947">
        <property type="entry name" value="DUF2628"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="120" mass="14265" checksum="50F44D29314AEC17" modified="2005-09-27" version="1">MQSFFIYKHPKKMNTLDSTVFVKDSFSFMTFIFSIFFTLYKKLWLLSFISISVFSVTYVMYSTLNLINLPIYLLINLLYSFYMASSYTDWYQAKLKKIGYKIHDVIFAENLISAKLKFKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR29</accession>
    <name>SYE2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate--tRNA ligase 2</fullName>
            <ecNumber evidence="1">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-tRNA synthetase 2</fullName>
            <shortName evidence="1">GluRS 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gltX2</name>
        <name type="ordered locus">Ecaj_0795</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304903.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YR29"/>
    <dbReference type="SMR" id="Q3YR29"/>
    <dbReference type="STRING" id="269484.Ecaj_0795"/>
    <dbReference type="PRIDE" id="Q3YR29"/>
    <dbReference type="EnsemblBacteria" id="AAZ68826">
        <property type="protein sequence ID" value="AAZ68826"/>
        <property type="gene ID" value="Ecaj_0795"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0795"/>
    <dbReference type="eggNOG" id="COG0008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_6_1_5"/>
    <dbReference type="OMA" id="PHWRFKL"/>
    <dbReference type="OrthoDB" id="1409413at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000237361" description="Glutamate--tRNA ligase 2">
        <location>
            <begin position="1"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="1">
        <location>
            <begin position="236"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00022"/>
        </source>
    </evidence>
    <sequence length="443" mass="51549" checksum="3508EE2D6663FD3D" modified="2005-09-27" version="1">MITRFAPSPTGYLHVGNVRTALICWLYVRKQKGKFLLRFDDTDTQRSQEEYIKEIENDLKWLNMNWDASFRQSSRFDRYEDVFQYLLKEGFLYPCYESKEELEFKRKMKLKSGLPPIYDRSALNLTQAEKDKYFGRAPYFRFKINQDQLINWDDEIRGKVSFNPKNISDPIIRRVDGTYTYMLPSVIDDMDFNVTHVIRGEDHISNTAVQIQMLDALKAKVPMFAHLSLLYSDDNKISKRVGGSSVKDMQLYELEPMAINSYFAKIGTSHPIDVHINMLGLINSFDITAFSQAPTKFNIDDILKLNPKILHNMSFDDVKDRLKELKIDKPAFWDFVCGNIEKFSDIEEWIKICSRDMVPVVKQDDKDFITLALNMFPQGEVHDSTWNTWVSNIKQQTDRRGKNLFAPLRLALTGLAAGPELAKLLPLIGREEIVRRLSYSVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR41</accession>
    <name>Q3YR41_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD kinase</fullName>
            <ecNumber evidence="5">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nadK</name>
        <name evidence="6" type="ordered locus">Ecaj_0783</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304891.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0783"/>
    <dbReference type="EnsemblBacteria" id="AAZ68814">
        <property type="protein sequence ID" value="AAZ68814"/>
        <property type="gene ID" value="Ecaj_0783"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0783"/>
    <dbReference type="eggNOG" id="COG0061">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073319_0_0_5"/>
    <dbReference type="OMA" id="NGIERMS"/>
    <dbReference type="OrthoDB" id="1463423at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="49"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="122"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="163"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="263" mass="29319" checksum="D70BDF5E10A98E03" modified="2005-09-27" version="1">MSKYKNIGYVFFDDFDKSVALTLEKQYGLIDIKKNKEAAIDLLVVIGGDGLMLHSLHNYIVSKYINIPVYGINYGTVGFLLNQYSDHNLIDRINQSVPTQLTVLNMVAIDTNKNQHNAIAINEVSLFRSTHQAANLQIKINSRLVMEKLVSDGILVSTPAGSTAYNFSAGGSILPLSSNVLSLTAINSFRPRRWRGAILTNSTIIEVDVINPKIRSVSAVADYTEFRNITNIKIKKDNDITITLLFDKGHNLEERIINEQFSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR47</accession>
    <name>SECB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-export protein SecB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secB</name>
        <name type="ordered locus">Ecaj_0777</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the proteins required for the normal export of preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer, a dimer of dimers. One homotetramer interacts with 1 SecA dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304885.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YR47"/>
    <dbReference type="SMR" id="Q3YR47"/>
    <dbReference type="STRING" id="269484.Ecaj_0777"/>
    <dbReference type="EnsemblBacteria" id="AAZ68808">
        <property type="protein sequence ID" value="AAZ68808"/>
        <property type="gene ID" value="Ecaj_0777"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0777"/>
    <dbReference type="eggNOG" id="COG1952">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111574_1_0_5"/>
    <dbReference type="OMA" id="CPNVLFP"/>
    <dbReference type="OrthoDB" id="1624074at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00821">
        <property type="entry name" value="SecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003708">
        <property type="entry name" value="SecB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035958">
        <property type="entry name" value="SecB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36918">
        <property type="entry name" value="PTHR36918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02556">
        <property type="entry name" value="SecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54611">
        <property type="entry name" value="SSF54611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00809">
        <property type="entry name" value="secB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000055366" description="Protein-export protein SecB">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00821"/>
        </source>
    </evidence>
    <sequence length="177" mass="19685" checksum="B5D25327BB4554F1" modified="2005-09-27" version="1">MSYKLKVKGQYIKDLSFENQNSPQIFVMISKTPPEINISVNVSSVSLPVKAQDQENGQSLDNKVEPLYEVTLQVNAEARVGTTVAFICEVKYCGVFSVENSDASNGEELSQQDMRDMLLISAPSILFPFVRELISRITATGGFPPLMLDVVDFKAMYESQIKQNAAEQNDNGQHTEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR65</accession>
    <name>RRF_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome-recycling factor</fullName>
            <shortName evidence="1">RRF</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosome-releasing factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">frr</name>
        <name type="ordered locus">Ecaj_0758</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304867.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YR65"/>
    <dbReference type="SMR" id="Q3YR65"/>
    <dbReference type="STRING" id="269484.Ecaj_0758"/>
    <dbReference type="EnsemblBacteria" id="AAZ68790">
        <property type="protein sequence ID" value="AAZ68790"/>
        <property type="gene ID" value="Ecaj_0758"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0758"/>
    <dbReference type="eggNOG" id="COG0233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073981_2_1_5"/>
    <dbReference type="OMA" id="FNPMNNG"/>
    <dbReference type="OrthoDB" id="1674344at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00520">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00040">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="PTHR20982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="SSF55194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00496">
        <property type="entry name" value="frr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000003159" description="Ribosome-recycling factor">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00040"/>
        </source>
    </evidence>
    <sequence length="185" mass="20853" checksum="6028735234544F89" modified="2005-09-27" version="1">MINEMKQDAKNRMEKTLSVYLSDIDGIRTGRARTSVLDGIVVETYGGRVKLNTISSISVSDNKTLMIKVWDSNNIGAIKTAIMNSNLGFGFSCEATTIRLTVPEMTQDMRKNLVKLLGKISEDCRVSVRNIRRDIMDKLKVMQDNKEISEDDLRVSGTEIQKITDDVMKKINDAFSSKEKELLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR80</accession>
    <name>Q3YR80_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0743</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304852.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0743"/>
    <dbReference type="EnsemblBacteria" id="AAZ68775">
        <property type="protein sequence ID" value="AAZ68775"/>
        <property type="gene ID" value="Ecaj_0743"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0743"/>
    <dbReference type="HOGENOM" id="CLU_2522295_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="84" mass="9630" checksum="98B0B69120EB9EBB" modified="2005-09-27" version="1">MRALKDKLACKEGDKSQSLYENLVSRIEQLNSIMRRVMDEEITGKNVQYMRMMSVTQLYILMQNISSVLNSISVQGVGDETLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR98</accession>
    <name>Q3YR98_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304834.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68757">
        <property type="protein sequence ID" value="AAZ68757"/>
        <property type="gene ID" value="Ecaj_0725"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0725"/>
    <dbReference type="HOGENOM" id="CLU_1425956_0_0_5"/>
    <dbReference type="OrthoDB" id="2183573at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="190" mass="21699" checksum="EC9F203546451780" modified="2005-09-27" version="1">MKDRLMIAAISLLAIFSFVLIMLVAISIYKCCILSNDRSENKILRDDIARLNTQLDILSRENKGLNSHIKDSSVESLQLSFELENFTKRMNDMEEVKPEDVKRMLFELLGKLDKFCKSVARKSENVEGNCETFIDMHLTKCKENILNMIDRLIQGENQVQQQTPQVLLDCNETQGVSALSCNSTNHVQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRA7</accession>
    <name>Q3YRA7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0716</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304825.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68748">
        <property type="protein sequence ID" value="AAZ68748"/>
        <property type="gene ID" value="Ecaj_0716"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0716"/>
    <dbReference type="HOGENOM" id="CLU_244274_0_0_5"/>
    <dbReference type="OrthoDB" id="676557at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1151"/>
            <end position="1182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1601" mass="176742" checksum="6D35B91CFCB64F64" modified="2005-09-27" version="1">MKSEIYVSILILLLLFILCVVVFYILKYCYNIQNIALVEDQKQQSLDFCAEEDQNAASEHANVEGSSDYRSEVEKLKSYSEDFDVYEYSYQVDQRKCSSDVANETYNFAAIARVADDVISSSIESSDGDFSISGCVNTIDQSKSRSVPKTYNSAVMARVADDVISSSIESSDGDFSISGCVNTIDQRKSRSVPKTYNSAVMARVADDVISSSIESSDGDFSISGCVNTIDQRKSRSVPKTYNSAVMARVADDVISSSIESSDGDFSISRCVNTIDQSKSRSVPKTYNSTVMARVADDVISSSIESSDGDFSISGCVNTIDQSKSRSVPKTYNSAVMARVADDVISSSIESSDGDFSISGCVNTIDQRKSRSVPKTYNSAVMARVADDVISSSIESSDGDFSISGCVNTIDQRKSRSVPKTYNSAVMARVADDVISSSIESSDGDFSISRCVNTIDQSKSRSVPKTYNSTVMARVADDVISSSIESSDGDFSISGCVNTIDQSKSRSVPKTYNSAVMARVADDVISSSIESSDGDFSISGCVNTIDQRKSRSVPKTYNSAVMARVADDVISSSIESSDGDFSISGCVNTIDQRKSRSVPKTYNSVAIANVAKDVVSCSSMGSSSVGFSISECDGLIGPKGYISDIVFETYNPAARINIAKDMIAYSSLESYGEYFWSFEVSECNTLIQPYCSEMMIGSFGNSKEFYNITFLDDCVASDIPENDTAIEYQEVHGFDYCDKVDNSCRNLIRSQTYDSDLSVASVNNIRLSNGNDVDFAAVRSDYFTSYGFNRRLSFHYGSKFARDNFSFANFMMYPTINSMAVYVKFEGIPIFHLHHSVISFFEGTRGLGSPGYYDVNGYSYTVMLQNVSLCFKTFRKLQKSRIFIVNQGDLINMKDHISNILQSNVKPVVSESQEIVCTAVRAEDNSRSSQSVVREPGVELKDITDSEAKVPFNDQEIGSANKSSVHVTTDPALDQILADALHYLKEFASSDASVRRVIEQDDASCSTIPAVTQFEESSEISEEVDISSKPGNDNLCDSRLLNDRTSSQGQETMHHMKNSIIVQAEVHGGAIPKRYRSNVPVECGESLGRKKLVERMPVYKWDDIEVISQTPDCGVSKKSVAGDRSDLFARGGFRTSGIPVSRRIASVMKLVKSSDGKKKQVVHQNNPVEKSRGDATGLSGAKSSEVKITEQVKDHDNGHNSQQTIHQDVRKSILKKSEIPVKAERSNSVSVAKHSVENIPDVATARHDRKDVRNKSLARSASTMHKGSSRFSLLDVVNPEGHTTAGKKVESVKVNPNVRRSKSNVADLRSQGSNTTDFVPSTINLGEIAILCDDGVSRTLIDILGEKFSIMRKLLLRLRNKGDADIQFKELLYIADSRIMYVNNLMRNSIDKWCMTQKTMLWDKYGKRIISECMEDMRNIFAKVDMQQDEVCVNSSKSIKDIRDFIEATVKWKIDFIVKNAISEVYIMVFNEAASTIYNKVCTAHRAMIKGANKYLQKSEEGKVVSSCLLFHKDTIRGNVLVHIEVLKTFFAKQLQLKSKEMVKGHLKKCEVNFVFDSVKDDCLRLVHAKYRFPVRDRFVGDMRCMLSSGTSVNEFASSQLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRB1</accession>
    <name>SYH_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="1">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisS</name>
        <name type="ordered locus">Ecaj_0712</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-histidyl-tRNA(His)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304821.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRB1"/>
    <dbReference type="SMR" id="Q3YRB1"/>
    <dbReference type="STRING" id="269484.Ecaj_0712"/>
    <dbReference type="EnsemblBacteria" id="AAZ68744">
        <property type="protein sequence ID" value="AAZ68744"/>
        <property type="gene ID" value="Ecaj_0712"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0712"/>
    <dbReference type="eggNOG" id="COG0124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_1_0_5"/>
    <dbReference type="OMA" id="CDFDFIG"/>
    <dbReference type="OrthoDB" id="277998at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136156" description="Histidine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <sequence length="413" mass="46999" checksum="98F94CAA14539C07" modified="2005-09-27" version="1">MQRSKLKEVRGTKDLLGAEFYKFQYIQHLSQAIANRYGFIAVDTPIIEFTEVFTKTLGDDSDIVTKEMYNFQDKSGENITLRPEFTSAIVRLLINKNLVTPVKLFSSGPVFRYERPQKCRQRQFHQVNFEFFGSDSPLADVEMIALGYNILSELKLLNNITLEINFLGDKETMNSYRLSLVEYLNKYKKDLSGDSQRRLITNPLRVLDSKSPEDCEILLNAPNIGDFYTKYSSDFFTEVLDGLNDLCIPYQLNNRIVRGLDYYCNTVFEFTTSELGSQNAVIAGGRYDGLVRSMGGNDTPAVGFAMGIERVSALIDYEHKESRNVVLVPIGKDAMSYALKLAYELRCKGISVGWNYKNTGLKNMLRKINDNSIVLIFGDEELKSNTVQVKDMKTGEQQEVEKGNLLDALYNKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-07-22" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRC9</accession>
    <name>RS18_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsR</name>
        <name type="ordered locus">Ecaj_0694</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein S6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304803.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRC9"/>
    <dbReference type="SMR" id="Q3YRC9"/>
    <dbReference type="STRING" id="269484.Ecaj_0694"/>
    <dbReference type="EnsemblBacteria" id="AAZ68726">
        <property type="protein sequence ID" value="AAZ68726"/>
        <property type="gene ID" value="Ecaj_0694"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0694"/>
    <dbReference type="eggNOG" id="COG0238">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148710_2_1_5"/>
    <dbReference type="OMA" id="QKKYCRF"/>
    <dbReference type="OrthoDB" id="1940575at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00270">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_S18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_S18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="PTHR13479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="SSF46911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00165">
        <property type="entry name" value="S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000345462" description="30S ribosomal protein S18">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00270"/>
        </source>
    </evidence>
    <sequence length="95" mass="10923" checksum="8234EDEB70481B45" modified="2005-09-27" version="1">MLKKNKKANSNNSNATAYSPLAYLKRPYFKRSKACPLEQCPNEDIDYKNKTLLSKFTSEYGRILPSRITSVSSRKQRLLSTAVKRARYLALLPYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRD4</accession>
    <name>Q3YRD4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0689"/>
    <dbReference type="EnsemblBacteria" id="AAZ68721">
        <property type="protein sequence ID" value="AAZ68721"/>
        <property type="gene ID" value="Ecaj_0689"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0689"/>
    <dbReference type="HOGENOM" id="CLU_2478424_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="87" mass="10298" checksum="2C1D04F895B0E103" modified="2005-09-27" version="1">MLTDNSSFLMYINVYALMLKDNVSNIQNYRYVNNIHQLSISNFLYVQLIYTRRFIMHISILIGMSILNMLSDVMLLICVHVNVMEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRD6</accession>
    <name>Q3YRD6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Succinate dehydrogenase iron-sulfur subunit</fullName>
            <ecNumber evidence="10">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ecaj_0687</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a quinone + succinate = a quinol + fumarate</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="10">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="10">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and a hydrophobic anchor protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304796.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0687"/>
    <dbReference type="EnsemblBacteria" id="AAZ68719">
        <property type="protein sequence ID" value="AAZ68719"/>
        <property type="gene ID" value="Ecaj_0687"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0687"/>
    <dbReference type="eggNOG" id="COG0479">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_0_2_5"/>
    <dbReference type="OMA" id="DGQYFGP"/>
    <dbReference type="OrthoDB" id="1605514at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="9 10" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="11">
        <location>
            <begin position="31"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="161"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="258" mass="29584" checksum="4ED5EDFC2774CB07" modified="2005-09-27" version="1">MVQFCLPKNSKINKNGKVHHAPENAKNVKCFKIYRWSPDDDENPRIDTFFIDLDECGQMVLDALIKIKNEVDSTLTFRRSCREGICGSCAMNIDGTNTLACTKAISDIKSDVKIYPLPHMYVIKDLVPDLSNFYAQYESVTPWMQAEEPNHNKERLQTIEDRSKLDGIYDCILCACCSTSCPSYWWNSDKYLGPAALLQAYRWLIDSRDEASNSRLDMLDDAFKLYRCHTIMNCTKTCPKGLNPAKAIAHIKQMMVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRE1</accession>
    <name>Q3YRE1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Heme exporter protein C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cytochrome c-type biogenesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ccmC</name>
        <name evidence="10" type="ordered locus">Ecaj_0682</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CcmC/CycZ/HelC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304791.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0682"/>
    <dbReference type="EnsemblBacteria" id="AAZ68714">
        <property type="protein sequence ID" value="AAZ68714"/>
        <property type="gene ID" value="Ecaj_0682"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0682"/>
    <dbReference type="eggNOG" id="COG0755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066538_2_1_5"/>
    <dbReference type="OMA" id="CGIIALW"/>
    <dbReference type="OrthoDB" id="955123at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002541">
        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003557">
        <property type="entry name" value="Cyt_c_biogenesis_CcmC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045062">
        <property type="entry name" value="Cyt_c_biogenesis_CcsA/CcmC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30071">
        <property type="entry name" value="PTHR30071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01386">
        <property type="entry name" value="CCMCBIOGNSIS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01191">
        <property type="entry name" value="ccmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrom_C_asm" evidence="9">
        <location>
            <begin position="17"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="234" mass="26712" checksum="363BC8891C160855" modified="2005-09-27" version="1">MSKFISYTRLALPYTVSLFFILLVCSLYMVFISSPPDYKQGEFVRIMYLHVPSAWISLGSYTLMAIFSFIALVWRNVISAILSHAIAPLGLTFTFICLITGSIWGKCTWGIWWVWDARLTSMLVLLFLYLGYLSLWNFFDNESRSMKAASIFAIFSAINIPVIKFSVNIWTTLHQPASIFRKSGIAIDISMLIPLLTMFATLSLLFCILLILRSHTLINLRKINSKLILLNNIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRF1</accession>
    <name>SYDND_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="1">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aspS</name>
        <name type="ordered locus">Ecaj_0672</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304781.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRF1"/>
    <dbReference type="SMR" id="Q3YRF1"/>
    <dbReference type="STRING" id="269484.Ecaj_0672"/>
    <dbReference type="EnsemblBacteria" id="AAZ68704">
        <property type="protein sequence ID" value="AAZ68704"/>
        <property type="gene ID" value="Ecaj_0672"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0672"/>
    <dbReference type="eggNOG" id="COG0173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014330_3_2_5"/>
    <dbReference type="OMA" id="YQLDVEM"/>
    <dbReference type="OrthoDB" id="226836at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="PTHR22594:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="SSF55261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000235526" description="Aspartate--tRNA(Asp/Asn) ligase">
        <location>
            <begin position="1"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="1">
        <location>
            <begin position="200"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="222"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="485"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="492"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="537"/>
            <end position="540"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="1">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00044"/>
        </source>
    </evidence>
    <sequence length="590" mass="67290" checksum="4EE4B53D78FC8107" modified="2005-09-27" version="1">MNIYRTHLCNELREEHINQEVILSGWVYRKRDHGKIIFVDLRDHYGITQLVFNEADNEIFQLISYLKLESVITIKGTVVARDSSTINTTVSTGLIEVVVNHVTIESEANLLPMNIASTQEYPEDIRFKYRFLDLRRDKVKHNIILRSKVIAELRKSMENMGFIEIQTPILTSSSPEGARDYLVPSRIHHGKFYALPQAPQLFKQILMVSGFDKYFQIAPCFRDEDARADRSPGEFYQLDMEMSFVTQEDVFNVIEPVLLNIFSKFSSKTIHKEFPRISYHNAMLYYGSDKPDLRNPLIIQDVTEIFRDSQFNIFNSNIKKGMVVRAIPAPKTANNPRSFFDNKIEFAKTLGAQGLGYITFNDDSSAKGPIAKFLDEERLNKIKSICNLQTGDSVFFVSETEDKAAEFAGEIRTILGTELNLIEPDTFRFCWVVDFPYFKYDKKEKSIGFFHNPFSMPQGGLEALNNQDPLSILAYQYDIVCNGIEISSGAIRNHKLDIMYKAFSIAGYTQEMVDKKFNSLTRAFKFGAPPHGGIAPGIDRMVMLLADATNIREVICFPLNQSGEDLLMGAPSEIDTEHLKLLSLNITKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRH5</accession>
    <name>Q3YRH5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-oxoacyl-[acyl-carrier-protein] reductase FabG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">3-ketoacyl-acyl carrier protein reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4 6">Beta-Ketoacyl-acyl carrier protein reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Beta-ketoacyl-ACP reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0646</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304757.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0646"/>
    <dbReference type="EnsemblBacteria" id="AAZ68680">
        <property type="protein sequence ID" value="AAZ68680"/>
        <property type="gene ID" value="Ecaj_0646"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0646"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_5"/>
    <dbReference type="OMA" id="SPIGHIE"/>
    <dbReference type="OrthoDB" id="1135906at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="241" mass="26667" checksum="64112673AD60C105" modified="2005-09-27" version="1">MEKQLAGKVAIITGASGNIGSAIAKRFAKEGACLILVAKNINKLNAIYDSIQESGGEAMLAAVDLQDFDTIKNFAMSIGTKFKCIDILVLNAAIVGALSPLQDYEYDLYKRIMDINFTANWHLIKYCDPLLKLSPAGRVIAVTSEVTQSLSRYPYWGPYASSKSALETMLRIYATETKHSKLCVNIVYPPLMYNDIYTQAFPTKEIAQSVLPENFTDKFIELASTQCNVTGETYKLESDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRH9</accession>
    <name>Q3YRH9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable cytosol aminopeptidase</fullName>
            <ecNumber evidence="8">3.4.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucine aminopeptidase</fullName>
            <shortName evidence="8">LAP</shortName>
            <ecNumber evidence="8">3.4.11.10</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Leucyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pepA</name>
        <name evidence="10" type="ordered locus">Ecaj_0642</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.</text>
            <dbReference type="EC" id="3.4.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.</text>
            <dbReference type="EC" id="3.4.11.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.1" evidence="8"/>
    <dbReference type="EC" id="3.4.11.10" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304753.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0642"/>
    <dbReference type="EnsemblBacteria" id="AAZ68676">
        <property type="protein sequence ID" value="AAZ68676"/>
        <property type="gene ID" value="Ecaj_0642"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0642"/>
    <dbReference type="eggNOG" id="COG0260">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013734_2_2_5"/>
    <dbReference type="OMA" id="HGFKRVC"/>
    <dbReference type="OrthoDB" id="356206at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00181">
        <property type="entry name" value="Cytosol_peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023042">
        <property type="entry name" value="Peptidase_M17_leu_NH2_pept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="PTHR11963"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTOSOL_AP" evidence="9">
        <location>
            <begin position="342"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="500" mass="54888" checksum="0A4C79F76144EC2C" modified="2005-09-27" version="1">MIDISFSSLMPGVSLLLKTTAIVVGIFEGSNHLEDYNALVDQRENIMKIVEDYKSFDGKFAEVLPIVGLDKNYPVVIVVGLGKAEEFDEEKSLKIGGVIYSELNRMKISEASIISSSDSDIMANIAYGAFLRSFRFNKYFVQKKDDNATYVSKLAFFSKSNPQKTSALFDDLKAEGESIFLARSFVSEPPNVLYPETYAQMIYEELSKVDVKVEIFDENYMRSNQMMALLGVGQGSAKKSRLVVMRWNGGRETEAPIAFVGKGVTFDTGGISLKPSKGMWDMKYDMAGSASVVGIMRTLAARKAKVNAVGVVGLVENAIGGDAQRPGDIVTSMSGQTIEVLNTDAEGRLVLADALWYTQKMFEPKLMIDLATLTGAVVVALGNNQYGGLFSNDDSIANQLIVAGNESGEKLWRLPLDDAYDKLINSSVADMQNISTKGYGADSITAAQFLQRFVNKTPWVHLDIAGMAWDNEGNEICPKGATGFGVRLLNRLILKYYEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRJ6</accession>
    <name>Q3YRJ6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304736.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68659">
        <property type="protein sequence ID" value="AAZ68659"/>
        <property type="gene ID" value="Ecaj_0625"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0625"/>
    <dbReference type="HOGENOM" id="CLU_2141961_0_0_5"/>
    <dbReference type="OMA" id="CILADCD"/>
    <dbReference type="OrthoDB" id="1777453at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="112" mass="13077" checksum="CCF24547421B28B8" modified="2005-09-27" version="1">MNYDTLNFYECDSTCDVEYTDCVRDNSVLDEYMSYQSMWKAVILQAIIDSTSNYRRMENKLEKIKATNWLNDFSRDFTTVCHFAGYNPLNVQNKAKKIISASKALNQNKSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRJ8</accession>
    <name>Q3YRJ8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Ferrochelatase</fullName>
            <ecNumber evidence="7 8">4.99.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Heme synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Protoheme ferro-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hemH</name>
        <name evidence="9" type="ordered locus">Ecaj_0623</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>2 H(+) + heme b = Fe(2+) + protoporphyrin IX</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.99.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Fe-coproporphyrin III + 2 H(+) = coproporphyrin III + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:49572"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:68438"/>
            <dbReference type="ChEBI" id="CHEBI:131725"/>
            <dbReference type="EC" id="4.99.1.9"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:49574"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.1" evidence="7 8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304734.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0623"/>
    <dbReference type="EnsemblBacteria" id="AAZ68657">
        <property type="protein sequence ID" value="AAZ68657"/>
        <property type="gene ID" value="Ecaj_0623"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0623"/>
    <dbReference type="eggNOG" id="COG0276">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018884_4_1_5"/>
    <dbReference type="OMA" id="FSYHGVP"/>
    <dbReference type="OrthoDB" id="780534at2"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="PTHR11108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="2 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="337" mass="39035" checksum="EF99797950B53413" modified="2005-09-27" version="1">MSKIAVVLFNLGGPQSLSEVRSFLFNLFYDKNIINLANPLRFILATLISFFRKKKAIGIYKHLNGQSPILKETKIQASSLENILNDNNDNQYQVFIFMRYSQPSAKDTVESVCQYNPDKIILLPLYPHYSTTTTLSAIQHWNYYMNKSNLQFSTSTVCCYYNNTNYIKAQCQLIIEKYQEAQKYGLPRVLFSAHSLPISIMQQGDPYQHQIEKNVLLITKFLNIPELDYKICYQSKVGPVKWLEPSTISEIKQAKQDNVPIVLVPISFVSENSETLVELDIDYKAIISKENMFFRVPTLSNNGYYIKCLKEICMQDHKNTCPTKCKMCFRYLTNSYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRK7</accession>
    <name>EFTU_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor Tu</fullName>
            <shortName evidence="2">EF-Tu</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tuf1</name>
        <name type="ordered locus">Ecaj_0162</name>
    </gene>
    <gene>
        <name evidence="2" type="primary">tuf2</name>
        <name type="ordered locus">Ecaj_0614</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304291.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRK7"/>
    <dbReference type="SMR" id="Q3YRK7"/>
    <dbReference type="STRING" id="269484.Ecaj_0162"/>
    <dbReference type="PRIDE" id="Q3YRK7"/>
    <dbReference type="EnsemblBacteria" id="AAZ68213">
        <property type="protein sequence ID" value="AAZ68213"/>
        <property type="gene ID" value="Ecaj_0162"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68648">
        <property type="protein sequence ID" value="AAZ68648"/>
        <property type="gene ID" value="Ecaj_0614"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0162"/>
    <dbReference type="KEGG" id="ecn:Ecaj_0614"/>
    <dbReference type="eggNOG" id="COG0050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_0_1_5"/>
    <dbReference type="OMA" id="EGDKEWG"/>
    <dbReference type="OrthoDB" id="621774at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000337374" description="Elongation factor Tu">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="6"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="15"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="59"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="173"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="15"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="80"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <sequence length="395" mass="43481" checksum="9530194A1407386D" modified="2005-09-27" version="1">MVEERKPHINVGTIGHVDHGKTTLTAALTTVLAKRLSGEGNKSVKYDEIDKAPEEKARGITISTAHVEYETENRHYAHVDCPGHADYIKNMITGAAQMDAAILVVSATDGAMPQTREHILLAKQVGVKDIVVWMNKCDVVDDEEMLSLVEMEIRELLSKYGYPGDDIDVVRGSAVKALEEETGSGVWSEKIMELMNALEKISLPVREKDKPFLMSIEDVFSIPGRGTVVTGRIERGVIRVGDKIEIVGLREIQSTVCTGVEMFHKALDAGEAGDNAGILLRGIKKEDVERGQVLSAPGQIHSYKRFKAEVYILKKEEGGRHTPFFSNYQPQFYVRTTDVTGNIKLPEGVEMVMPGDNINIEVSLDKPVAIDQGLRFAIREGGRTVGSGIITEILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRL1</accession>
    <name>Q3YRL1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplW</name>
        <name evidence="6" type="ordered locus">Ecaj_0610</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit. Contacts protein L29, and trigger factor when it is bound to the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304722.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0610"/>
    <dbReference type="EnsemblBacteria" id="AAZ68644">
        <property type="protein sequence ID" value="AAZ68644"/>
        <property type="gene ID" value="Ecaj_0610"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0610"/>
    <dbReference type="eggNOG" id="COG0089">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037562_3_1_5"/>
    <dbReference type="OMA" id="GQKLDIM"/>
    <dbReference type="OrthoDB" id="1978865at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_B">
        <property type="entry name" value="Ribosomal_L23_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_L25/23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="96" mass="11195" checksum="9D1007B69BC8DF4E" modified="2005-09-27" version="1">MINYFNVIQSSIITEKAVLLNEKFNKYAVYVSVDANKQKIKKALKVVFDLDATHINVINQKGKKKHFKGIVGHRKDRKKVYFSLPKDQSFKYMGMK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRM7</accession>
    <name>RL15_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplO</name>
        <name type="ordered locus">Ecaj_0594</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304706.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRM7"/>
    <dbReference type="SMR" id="Q3YRM7"/>
    <dbReference type="STRING" id="269484.Ecaj_0594"/>
    <dbReference type="EnsemblBacteria" id="AAZ68628">
        <property type="protein sequence ID" value="AAZ68628"/>
        <property type="gene ID" value="Ecaj_0594"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0594"/>
    <dbReference type="eggNOG" id="COG0200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055188_4_0_5"/>
    <dbReference type="OMA" id="FKNINRV"/>
    <dbReference type="OrthoDB" id="1874945at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_L15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="PTHR12934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000251508" description="50S ribosomal protein L15">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="156" mass="16813" checksum="301D7F03461E9879" modified="2005-09-27" version="1">MNTVVKLNNIFSGLPKKKKTKVLGRGIGCGKGKTSGRGHKGQKARSGVSINGFEGGQQSIFTRLPKRGFNSLFKSKYSIVNLSTIQRLIDSKKIENVSAITKQVLFNLGVIPSIKEKIKILGDGKLNTAVAIEYDFISKSAGSQVTLLSSVSEDKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRN3</accession>
    <name>RL17_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplQ</name>
        <name type="ordered locus">Ecaj_0588</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L32.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304700.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRN3"/>
    <dbReference type="SMR" id="Q3YRN3"/>
    <dbReference type="STRING" id="269484.Ecaj_0588"/>
    <dbReference type="EnsemblBacteria" id="AAZ68622">
        <property type="protein sequence ID" value="AAZ68622"/>
        <property type="gene ID" value="Ecaj_0588"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0588"/>
    <dbReference type="eggNOG" id="COG0203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074407_2_0_5"/>
    <dbReference type="OMA" id="YAVTELF"/>
    <dbReference type="OrthoDB" id="1838961at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01368">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_L17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="PTHR14413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="SSF64263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01167">
        <property type="entry name" value="RIBOSOMAL_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000055818" description="50S ribosomal protein L17">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01368"/>
        </source>
    </evidence>
    <sequence length="132" mass="15056" checksum="A3852CCC45635C87" modified="2005-09-27" version="1">MRHRVAHRKFSRTSAHRMAMLLNMCISLIKHERISTTLPKAKELRPYVEKLITIGKVYHGKNLVYGKRLLISKIKNPDAANKLIDVLSIRYKSRNGGYTRIIKNGFRKGDSAPMAIIELVDRQIATVENNGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRQ6</accession>
    <name>PURA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylosuccinate synthetase</fullName>
            <shortName evidence="1">AMPSase</shortName>
            <shortName evidence="1">AdSS</shortName>
            <ecNumber evidence="1">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purA</name>
        <name type="ordered locus">Ecaj_0564</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304677.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRQ6"/>
    <dbReference type="SMR" id="Q3YRQ6"/>
    <dbReference type="STRING" id="269484.Ecaj_0564"/>
    <dbReference type="EnsemblBacteria" id="AAZ68599">
        <property type="protein sequence ID" value="AAZ68599"/>
        <property type="gene ID" value="Ecaj_0564"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0564"/>
    <dbReference type="eggNOG" id="COG0104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_0_0_5"/>
    <dbReference type="OMA" id="FHHAKPI"/>
    <dbReference type="OrthoDB" id="232152at2"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224277" description="Adenylosuccinate synthetase">
        <location>
            <begin position="1"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="299"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="331"/>
            <end position="333"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="413"/>
            <end position="415"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <sequence length="431" mass="48012" checksum="377C825D36F69580" modified="2005-09-27" version="1">MVNIIVVGLQWGDEGKGKVVDWLSTNADAVVRFQGGNNAGHTIVVNDNVYKLNLLPSSVLQNGKLSIIGNGVVLDPYALISEIENLKSNGINITSQNFAISESCPLVLSVHKQADMLFEQLRQDTIGTTNKGIGPCYADKISRRAIRVCDLFDPKDLLHNKVNHLLSYHNLLRKNINNPSIETENIVNELLSIAPKILPFVQPVWKTIHNLIEQNKTIIFEGAQGTFLDIDHGTYPFVTSSNTIAQQAFIGCGINPSNKTHILGLVKAYTTRVGNGPFFTEQNNNIGKTMFESGKELGTVSNRQRRCGWFDAVLARQAVILSGVSGLVMTKLDVLDQFSEIKICVKYKYENKIYDYLPASPYIQSKLEPVYETLPGWQTSTFGSVSYKDLPQNAISYIKKIEEILKVPIYLISTGPERNSMIIINNDFLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRR4</accession>
    <name>Q3YRR4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Multisubunit sodium/proton antiporter, MrpC subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0556</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304669.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0556"/>
    <dbReference type="EnsemblBacteria" id="AAZ68591">
        <property type="protein sequence ID" value="AAZ68591"/>
        <property type="gene ID" value="Ecaj_0556"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0556"/>
    <dbReference type="eggNOG" id="COG1006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082058_2_0_5"/>
    <dbReference type="OMA" id="KRHGTVD"/>
    <dbReference type="OrthoDB" id="2078994at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="111" mass="12039" checksum="5FF81AAFDC831A24" modified="2005-09-27" version="1">MIFGYFNYVVSIILMVIGLYVTAADKNLVKKLIGLNVFQTSVLLFYISIGYVSNGTVPILTDGAILYTNPLPSVLMLTAIVVGVAILAVGLSIIVKIKKAFASIDDEKIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-04-07" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRS4</accession>
    <name>Q3YRS4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uroporphyrinogen III synthase HEM4</fullName>
            <ecNumber evidence="2">4.2.1.75</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.75" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0546"/>
    <dbReference type="EnsemblBacteria" id="AAZ68581">
        <property type="protein sequence ID" value="AAZ68581"/>
        <property type="gene ID" value="Ecaj_0546"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0546"/>
    <dbReference type="eggNOG" id="COG1587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_10_1_5"/>
    <dbReference type="OMA" id="LAIPQCC"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033014">
        <property type="term" value="P:tetrapyrrole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="SSF69618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEM4" evidence="1">
        <location>
            <begin position="21"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="240" mass="27683" checksum="E9C81CDF179F1499" modified="2005-09-27" version="1">MQFYYMKSLLVTRPEKDSIKIKESLNKLGFNVYIEPMFSIKYLTAKITLKDFDLIICTSQHSIIALSKITKNRDITIITVGNNTMHIAQELGFTFVKSLNGNINDVVSYLQNYSKSNILYIRGKEVTYNLKKTFNHYKNFQEIILYNTIDRTHFSKCCYSAFLGNKISGVLLYSSRTAEILIELIKKYNIQDKISNITAYTMSNKIANTAKNVHWKTIKISDQPTNESLLSLISNNIRNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRS7</accession>
    <name>RL28_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmB</name>
        <name type="ordered locus">Ecaj_0542</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304656.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRS7"/>
    <dbReference type="SMR" id="Q3YRS7"/>
    <dbReference type="STRING" id="269484.Ecaj_0542"/>
    <dbReference type="EnsemblBacteria" id="AAZ68578">
        <property type="protein sequence ID" value="AAZ68578"/>
        <property type="gene ID" value="Ecaj_0542"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0542"/>
    <dbReference type="eggNOG" id="COG0227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064548_4_2_5"/>
    <dbReference type="OMA" id="MARRCEL"/>
    <dbReference type="OrthoDB" id="2062238at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00373">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribo_L28/L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037147">
        <property type="entry name" value="Ribo_L28/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001383">
        <property type="entry name" value="Ribosomal_L28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528">
        <property type="entry name" value="PTHR13528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00009">
        <property type="entry name" value="L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000007230" description="50S ribosomal protein L28">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="100" mass="11317" checksum="D6885F7A02B03C0F" modified="2005-09-27" version="1">MSRVCDITGQGKSFGNKVSHSNRKTKRTYLVNLHNVTLLSDILNRKFKFKVSSRTLRTIDYKGGFDLYLLNTSSRKLTDKAQKIKKLVKNAVAKGVKVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRS8</accession>
    <name>Q3YRS8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="2">DAP decarboxylase</shortName>
            <shortName evidence="2">DAPDC</shortName>
            <ecNumber evidence="2 3">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lysA</name>
        <name evidence="6" type="ordered locus">Ecaj_0541</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="2 3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304655.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0541"/>
    <dbReference type="EnsemblBacteria" id="AAZ68577">
        <property type="protein sequence ID" value="AAZ68577"/>
        <property type="gene ID" value="Ecaj_0541"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0541"/>
    <dbReference type="eggNOG" id="COG0019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_0_0_5"/>
    <dbReference type="OMA" id="YCRSMAS"/>
    <dbReference type="OrthoDB" id="861683at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="5">
        <location>
            <begin position="40"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="4">
        <location>
            <begin position="283"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="276"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="421" mass="47014" checksum="07477A4D1AEDF2D1" modified="2005-09-27" version="1">MIFLANPFFHYKNNILNIENVNVLDIAKDIGTPVYCYSLNAIKNNYRQFQENLPNNSIICYAVKSNSNLSILSLLNSLGAGADVVSEGEIRRAIAVGISPNKIVFSGVGKTEQEISFALDNRILQFNVESIEELSLINTIACKKNIIAPVSIRINPNISADTNDKITTGLKVNKFGIPEELLDQIFNNNFIGIRIIGISVHIGSQISNLNIFQNTIDKIKKIVKIFEQRNMQIIRVDLGGGLGIPYKNADVFPTIPEYANLLKKNFENENYQIICEPGRALIGNTGVLLTKVLYHKHNQEKSHIILDAGMNDLIRPALYNAEHTIIPAISTSTPLQLYDIVGPICESDDTFAYNYHINNLQNGEIVAICTTGAYGSSMSSNYNSRPLVPEVMVSNSNYDIIRHRQTYEDMLKDEVILDFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRU4</accession>
    <name>Q3YRU4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Carbamoyl-phosphate synthase small chain</fullName>
            <ecNumber evidence="7">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Carbamoyl-phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">carA</name>
        <name evidence="9" type="ordered locus">Ecaj_0525</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304639.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0525"/>
    <dbReference type="EnsemblBacteria" id="AAZ68561">
        <property type="protein sequence ID" value="AAZ68561"/>
        <property type="gene ID" value="Ecaj_0525"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0525"/>
    <dbReference type="eggNOG" id="COG0505">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035901_2_1_5"/>
    <dbReference type="OMA" id="CFNTGMT"/>
    <dbReference type="OrthoDB" id="662268at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="SSF52021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CPSase_sm_chain" evidence="8">
        <location>
            <begin position="8"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="371" mass="41119" checksum="0EF2B468DCCA2816" modified="2005-09-27" version="1">MSQLTSYYNAVLVLSDGKHFFGKSIGSRIPTVGEVCFTTSMIGYQHTITDPSFAGQIITFTFPHIGNVGINHKDFENHNILTHGIIVKTISEDSHSRSYSNLEHWMIQNNLTGISEIDTRALTCHLRNNGSQNGVIYHFDDINLINLSELQKQASRYNYTKHCLTIKFKNNDRTTDDNTHLYNIVVVNFGVKSGILDALSELGCKIHMISGNEDNLSKKTLSLKPQGIVLSNGPGDPSAVSENTIEEIKTLIESKIPILAICLGHQLISLAIGAKITKMLFGHRGSNHPVYNKINNNIEITSQNHGFVVNEDSLPTSAQVTHRSLFDNTIEGIQVDGYPIISVQYHPEGNPGPNDSSYIFDNFINLIKANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRU7</accession>
    <name>Q3YRU7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Quino protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0522</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304636.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0522"/>
    <dbReference type="EnsemblBacteria" id="AAZ68558">
        <property type="protein sequence ID" value="AAZ68558"/>
        <property type="gene ID" value="Ecaj_0522"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0522"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_785135_0_0_5"/>
    <dbReference type="OMA" id="GRVLWQR"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="387" mass="42778" checksum="996D392B65E82C60" modified="2005-09-27" version="1">MIKSGIVTLIVLILLFPVLVLASGVVQEHSLYDVEMSDNSGGNHDKDVAVLASSRLSNRDVISLKMHQGVMPVFVNDRIIFLSRNGILHSVDSKNIEKCYWKLDLTKYSKMYRANILYSENMVFYIVDDNVYGIDFETGEIKWQKGLSSIIAGSPIVADNNLIVVTVDNNLSSFNVSDGSLLWSSQESLPEVKSYSSASSALYGNTVIFSFSNGKVIAFNYLNGLKMWESNVSKANIAFSNGAALQAANGTVIAVDKLHSISNIDIESGKTRWSKDLKVEYVSQIDGGNIAAIIDNKLVLLDVSTGNFLWQYDLLQHGKPKNKWCAPIIIGDKILVISNDGCMVYLDYKSGTLKMVNYILSSTYYVPVFLNSSVYIVTDKGKVVIFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRX0</accession>
    <name>Q3YRX0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">DNA repair protein RecO</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Recombination protein O</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">recO</name>
        <name evidence="9" type="ordered locus">Ecaj_0499</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in DNA repair and RecF pathway recombination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the RecO family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0499"/>
    <dbReference type="EnsemblBacteria" id="AAZ68535">
        <property type="protein sequence ID" value="AAZ68535"/>
        <property type="gene ID" value="Ecaj_0499"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0499"/>
    <dbReference type="eggNOG" id="COG1381">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086029_0_0_5"/>
    <dbReference type="OMA" id="AYVSPKS"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00201">
        <property type="entry name" value="RecO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022572">
        <property type="entry name" value="DNA_rep/recomb_RecO_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003717">
        <property type="entry name" value="RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042242">
        <property type="entry name" value="RecO_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33991">
        <property type="entry name" value="PTHR33991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02565">
        <property type="entry name" value="RecO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11967">
        <property type="entry name" value="RecO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="SSF57863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00613">
        <property type="entry name" value="reco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_rep/recomb_RecO_N" evidence="8">
        <location>
            <begin position="6"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="248" mass="28911" checksum="C569C23C0C0BD2F5" modified="2005-09-27" version="1">MSFSVRWQDQGIVIAIRKYGDDQIIISIFTQKHGLKKGLARYQKKTIHRLQIGDHVNVTWSAKLISNLGYFKYEIIKSTLHHYIHNNLKTMCIAFFTSILDQVLPENEENYTIYNHLDIFMNSVQNKDVNWQIKYLTLELTLLSELGFGLDLSQCAVNNTNENLIFISPKTGKAISQTVGLPYKKSLLPLPPLLYNVYNNYTYKYSKTDFKVSLNVTGYFLKKHFLTEKNITLIKYRDEIIKLIDLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRY4</accession>
    <name>Q3YRY4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0484</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304599.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68521">
        <property type="protein sequence ID" value="AAZ68521"/>
        <property type="gene ID" value="Ecaj_0484"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0484"/>
    <dbReference type="HOGENOM" id="CLU_118039_0_0_5"/>
    <dbReference type="OMA" id="GANSEMN"/>
    <dbReference type="OrthoDB" id="2120040at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="198" mass="23252" checksum="F1B4FEB3FCED20DA" modified="2005-09-27" version="1">MDLRLLLLKCGLPILLPTNTCIYNTESGMYYTLDMHMNARLAPTLQSSDDDQFHRIYGVRFTRYTAVCSITLNQHVVLMFGNNQSSGELAFLVVRMPRYLMRDASLRGLMLNRNDLLTYGCISELQDVISYEDYTLMLFDKFKEHMKINCHTPDPRTHILDFYNDDDRLFCISYFNTVLHDVKVNELTNKQIYVMKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRZ0</accession>
    <name>Q3YRZ0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit J</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 6 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304593.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0478"/>
    <dbReference type="EnsemblBacteria" id="AAZ68515">
        <property type="protein sequence ID" value="AAZ68515"/>
        <property type="gene ID" value="Ecaj_0478"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0478"/>
    <dbReference type="eggNOG" id="COG0839">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085957_5_1_5"/>
    <dbReference type="OMA" id="MLVNKRE"/>
    <dbReference type="OrthoDB" id="1683794at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001457">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00499">
        <property type="entry name" value="Oxidored_q3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0874">Quinone</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="200" mass="22448" checksum="5D6032442D7C3898" modified="2005-09-27" version="1">MVYFLFHFFAALIVLSSVAVVYISNPVYSVLFLILTFFISSTLFILLGAELIAMLVIIVYVGAVAVLFLFVVMMLDINYSRFKNVFTKYLAIGLLCGVILFVNIVFIIRQSATTELVTESVQYVSNVVAIGNLIYTEYMYIFHLSGILLLISIIGALVLTLRAKNSSVHRQNINMQINRPSSIKYVSVKVKEGVNYEDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS00</accession>
    <name>Q3YS00_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription termination/antitermination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nusA</name>
        <name evidence="9" type="ordered locus">Ecaj_0468</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Participates in both transcription termination and antitermination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304583.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0468"/>
    <dbReference type="EnsemblBacteria" id="AAZ68505">
        <property type="protein sequence ID" value="AAZ68505"/>
        <property type="gene ID" value="Ecaj_0468"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0468"/>
    <dbReference type="eggNOG" id="COG0195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029242_0_0_5"/>
    <dbReference type="OMA" id="MDIDMSA"/>
    <dbReference type="OrthoDB" id="384865at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1480.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_B">
        <property type="entry name" value="NusA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025249">
        <property type="entry name" value="KH_dom_NusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="NusA_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036555">
        <property type="entry name" value="NusA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013735">
        <property type="entry name" value="TF_NusA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010214">
        <property type="entry name" value="Tscrpt_termin_fac_NusA_C_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010213">
        <property type="entry name" value="Tscrpt_termination_fac_NusA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="PTHR22648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13184">
        <property type="entry name" value="KH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08529">
        <property type="entry name" value="NusA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="SSF47794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69705">
        <property type="entry name" value="SSF69705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01953">
        <property type="entry name" value="NusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01954">
        <property type="entry name" value="nusA_Cterm_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="5 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 7" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="158"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="517" mass="57600" checksum="F91227E3717D3283" modified="2005-09-27" version="1">MVNLQNFDNLELIRVAKDIADQKGLNLDIIIRAIEEAIQLTSRSKYGNCKIKVTVDRKTGVVSTYRQVLVINDNGEIVIPENKKSEIDSSDISKYKLITLTEAKQIDENAEVGDIMLEHLPVIDLDYSSAKIAKQKIAQVIISEERKKQYEDFKDRIGDIVYGTAKRIEYNNVIVDLNGNEGYLSAANLIKGEVFRVGDRVKAHIEDVRKENSGPQIFLSRISKGFMEQLFKQEIPEIYDGIVTIKAIARDPGSRSKVAVFSSDKNIDPVGACVGARGVRIQSIIYELHGEKIDVILYSPELAKFIVNAIAPAEVLKVIIDEDKEKVELIIPENQLSLAIGRYGQNIRLASELIGWKIDVIGDETESSRKAKELSEGSKIFVEGLDVEEIIGQLLFTEGFVSIEDIDQASVSDIAAIDGFNEEIAEELKSRAADYLTRKDAEIKQILDNLSVDKDVTTLPFLKPSDIIALSENGIKSLEDIAGLCTDEFFDIVPNIALTKDQIDSVILESRKKIGWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS06</accession>
    <name>Q3YS06_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2312 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0462</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304577.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0462"/>
    <dbReference type="EnsemblBacteria" id="AAZ68499">
        <property type="protein sequence ID" value="AAZ68499"/>
        <property type="gene ID" value="Ecaj_0462"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0462"/>
    <dbReference type="eggNOG" id="COG3750">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158651_3_0_5"/>
    <dbReference type="OMA" id="GNGYDVK"/>
    <dbReference type="OrthoDB" id="1940079at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046367">
        <property type="entry name" value="GapR-like_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10073">
        <property type="entry name" value="DUF2312"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2312" evidence="2">
        <location>
            <begin position="17"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="93" mass="11151" checksum="B365E6FFC1CA6059" modified="2005-09-27" version="1">MNISNLTSQNENNVVIENLKRYIERIEKLESEREEISQYIRKVYNEANSNGFNSKVMRQIVKLRKMDNDDREEHEMLLMTYKRALGILVEVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS10</accession>
    <name>Q3YS10_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Magnesium transporter MgtE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0458</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304573.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0458"/>
    <dbReference type="EnsemblBacteria" id="AAZ68495">
        <property type="protein sequence ID" value="AAZ68495"/>
        <property type="gene ID" value="Ecaj_0458"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0458"/>
    <dbReference type="eggNOG" id="COG2239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037408_1_0_5"/>
    <dbReference type="OMA" id="TMTVAVR"/>
    <dbReference type="OrthoDB" id="1239195at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006668">
        <property type="entry name" value="Mg_transptr_MgtE_intracell_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038076">
        <property type="entry name" value="MgtE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006669">
        <property type="entry name" value="MgtE_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43773">
        <property type="entry name" value="PTHR43773"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03448">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00924">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="SSF161093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00400">
        <property type="entry name" value="mgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="8">
        <location>
            <begin position="212"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="456" mass="51167" checksum="2E63965EF189CB99" modified="2005-09-27" version="1">MYTQESDNALHYILDEKISNSLTEALEKQNYNVIEEIINKSDSVQLAYFLLTSTYQHRKEFLNNINDNLVKDILIHLVPDLRQEIIKILGIKKVASLISQLNTEDIVAVIEDLDKESQVNILNILPKSNKIHVHELLSYPEESAGRLMHKDIIIAPEYWTISQLLTFLKQNNKLSKEFYEIFIVDPKLNPIGYLRLDNIICSNSTQIIKNLMKTDIKIIKSHTTQEEVAEIFRKYSLLSAPVINKDGRIIGSIVVHDILNVMHQEAEKDVLHLGMVSNNDISSSLIKTVIKRLPWLFINLLTATVSSMIIGIFSNTIQHFIALSVIMPIIASMSGNAGLQALTVTIRALSTKQLTNRNANRLLFKELSVGFINGLILAIISSIALMLRGQHVDIQILSGMSMIITFSIATFLGTAIPMILNLFKVDPAISSSIIICATSDTLSFLIFLGMATIFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS33</accession>
    <name>Q3YS33_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304550.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0430"/>
    <dbReference type="EnsemblBacteria" id="AAZ68472">
        <property type="protein sequence ID" value="AAZ68472"/>
        <property type="gene ID" value="Ecaj_0430"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0430"/>
    <dbReference type="HOGENOM" id="CLU_083566_0_0_5"/>
    <dbReference type="OMA" id="TEPLICY"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021387">
        <property type="entry name" value="DUF3023"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11224">
        <property type="entry name" value="DUF3023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="183" mass="20974" checksum="C3519C78F0BAFA74" modified="2005-09-27" version="1">MSKVKIDPNIYCIGNTVESTNRLQVHVSNKRHNRTQVQPIGNSLFLLKARFPSGLVKNDSHLRSIYALSNKEKEELSIAANLYCLVKKDALAEFNILKEKILNKEQKYINYFINYGNIVFANLTIDSNPKSQYNEHFALQNIAQLDADFIVEQEQNTTLSIPKSRMRIGKLDTIEEENSNLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS34</accession>
    <name>Q3YS34_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304549.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0429"/>
    <dbReference type="EnsemblBacteria" id="AAZ68471">
        <property type="protein sequence ID" value="AAZ68471"/>
        <property type="gene ID" value="Ecaj_0429"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0429"/>
    <dbReference type="HOGENOM" id="CLU_1105778_0_0_5"/>
    <dbReference type="OMA" id="YCIGHTA"/>
    <dbReference type="OrthoDB" id="2115157at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021387">
        <property type="entry name" value="DUF3023"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11224">
        <property type="entry name" value="DUF3023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="251" mass="28229" checksum="77B44520E4E8CBA7" modified="2005-09-27" version="1">MLVPQEVIASNSLLCEYVKYTTINIKSATCIGSTDIINNKLKIHISKDSNLQPIVQPHAKSLFLLEVTIPSSIVKNDPYLRNIYIISQSESLDANLYCLVDASHIQSFTNEIYDTVQNKVPKFVDNIFKYTEVIFAKLEIDSHLKLNEYEILCSILPDAEFIIHEIEEKNSCCSCCPPFTSKQNITRVQSQESLLQENDAEQVTLPKPEDNITNTSISSTDSSTEHNSISSLLSFVNVTIESINSFQNNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS36</accession>
    <name>Q3YS36_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase subunit E</fullName>
            <ecNumber evidence="7">1.6.5.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0427</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="6">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.3" evidence="7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304547.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0427"/>
    <dbReference type="EnsemblBacteria" id="AAZ68469">
        <property type="protein sequence ID" value="AAZ68469"/>
        <property type="gene ID" value="Ecaj_0427"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0427"/>
    <dbReference type="eggNOG" id="COG1905">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054362_2_0_5"/>
    <dbReference type="OMA" id="MYDTKPV"/>
    <dbReference type="OrthoDB" id="1206880at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="180" mass="20256" checksum="62FE189C01A77C28" modified="2005-09-27" version="1">MSSTQKNSNTNYIGEFKFSKENLKHAKDTINKYPQDRKSSAVMALLHIAQKQCGGFIPSSAINYIADFLGMQLIHVYEVAKFYSMYNLSVTGKYLVQVCRTTPCWLCGSDDVLKSCKELLNIGIGETTSDNLFTLKEVECLGACVNAPVVQINDDYYEKLTPDKMKDILLEIQKKENLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS55</accession>
    <name>Q3YS55_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein DnaJ, N-terminal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0407</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Co-chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Seems to help targeting proteins to be folded toward HscA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HscB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304528.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0407"/>
    <dbReference type="EnsemblBacteria" id="AAZ68450">
        <property type="protein sequence ID" value="AAZ68450"/>
        <property type="gene ID" value="Ecaj_0407"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0407"/>
    <dbReference type="eggNOG" id="COG1076">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068529_2_2_5"/>
    <dbReference type="OMA" id="SMEIREY"/>
    <dbReference type="OrthoDB" id="1520143at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004640">
        <property type="entry name" value="HscB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036386">
        <property type="entry name" value="HscB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009073">
        <property type="entry name" value="HscB_oligo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021">
        <property type="entry name" value="PTHR14021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07743">
        <property type="entry name" value="HSCB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47144">
        <property type="entry name" value="SSF47144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00714">
        <property type="entry name" value="hscB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="3"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="145" mass="17029" checksum="2F378209EEC31878" modified="2005-09-27" version="1">MYKDYFSLFKLKQEFCINIQVLEQNYIALQSNYHPDLFFLKSEKKLALDNIAEINKAYQILKSSITRAEYLLQIKGITADKNDINSIIEEIFKIQESNNIDIEYQISSATKAMEDAFSREDFNEAAKQVIKLKYLRKIQEDRGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS56</accession>
    <name>Q3YS56_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein Hsp70</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0406</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304527.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0406"/>
    <dbReference type="EnsemblBacteria" id="AAZ68449">
        <property type="protein sequence ID" value="AAZ68449"/>
        <property type="gene ID" value="Ecaj_0406"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0406"/>
    <dbReference type="eggNOG" id="COG0443">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_2_3_5"/>
    <dbReference type="OMA" id="IRVEVTF"/>
    <dbReference type="OrthoDB" id="161217at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010236">
        <property type="entry name" value="ISC_FeS_clus_asmbl_HscA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="SSF100920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="SSF100934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01991">
        <property type="entry name" value="HscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="618" mass="68910" checksum="19E02F6F73565108" modified="2005-09-27" version="1">MQLINITEPKTNENTLAFGIDLGTTNSLISMIDNEGKTIIFSDENGRFLIPSTVSYINNQVEVGKNYSNDNTISSIKRLMGKSIKDINELSSELLFNITDQGNNNIYIKKQDGTYVTPVEVSAEILKKLCKIVKDSTNLEVKKVVITVPAYFDEAARKATKDAAHLANLEVLRLLNEPTAAALAYGIEKPEYENNIYMVYDLGGGTFDVSILKLHQGVFQVLATGGDTNLGGDDIDYLLAKFIYNKYQKEENINNIEFNKELLSYLIPDVKHAKEYLSENYSGSFTFKIHEKNFSCKISRDELQDIISDLLNKTLSIITSTINSIELDFNSIAKVILVGGATKMPIIKNMLNNIFHNKVFCDIDPEKIVAVGAALQAYYLSNPHIKNKHVLIDALPLSLGIETMGGIVEKIIPRNTPIPASATQEFTTYIDGQNSIQIHVCQGEREMVEHNKSLAKFDLKGIPPLPAGSAKIIVEFNVDMDGMLTVSAQEKSTGIKQSVSINNTCELDQKEIEDNIIDSINNFDSDMQMRLIAEQKVEGQRIITIIKNALIADKNLISEKEFNIINHAITEAEKTLINGNIDKINDAINNVEVLADDFIKKRMDFYLKKSIDNCNINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS76</accession>
    <name>Q3YS76_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sigma 54 modulation protein/ribosomal protein S30EA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0386</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304507.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0386"/>
    <dbReference type="EnsemblBacteria" id="AAZ68429">
        <property type="protein sequence ID" value="AAZ68429"/>
        <property type="gene ID" value="Ecaj_0386"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0386"/>
    <dbReference type="eggNOG" id="COG1544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071472_0_1_5"/>
    <dbReference type="OMA" id="RRLKDHH"/>
    <dbReference type="OrthoDB" id="1766263at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00552">
        <property type="entry name" value="RaiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003489">
        <property type="entry name" value="RHF/RaiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032528">
        <property type="entry name" value="Ribosom_S30AE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038416">
        <property type="entry name" value="Ribosom_S30AE_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16321">
        <property type="entry name" value="Ribosom_S30AE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02482">
        <property type="entry name" value="Ribosomal_S30AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="SSF69754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00741">
        <property type="entry name" value="yfiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="1" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Ribosom_S30AE_C" evidence="2">
        <location>
            <begin position="139"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="197" mass="22314" checksum="6137E6A2F92C9893" modified="2005-09-27" version="1">MNIIITARGFDVTDNIKTYVERSVQDYITKFFADDSTVSVKVTLSKDANLFNSTISISDIKSEFIRISKTSDTAYHAIDNSIDYLRDKLQKHKTEKINKYRHNKNSVKAQMKCFGYVFDSNRVESYNVDNGDGSGLVIEEDVMVKTMTIGDAIMEMELLSLPALLFINVKNNRVNMIYTNSSGNVVWVDPLNISAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS80</accession>
    <name>Q3YS80_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized phage protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0381</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304503.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0381"/>
    <dbReference type="EnsemblBacteria" id="AAZ68425">
        <property type="protein sequence ID" value="AAZ68425"/>
        <property type="gene ID" value="Ecaj_0381"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0381"/>
    <dbReference type="eggNOG" id="ENOG50319N0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085951_0_1_5"/>
    <dbReference type="OMA" id="YAQWYIE"/>
    <dbReference type="OrthoDB" id="1829390at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd08054">
        <property type="entry name" value="gp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021146">
        <property type="entry name" value="Phafe_gp6_head-tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011738">
        <property type="entry name" value="Phage_CHP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05135">
        <property type="entry name" value="Phage_connect_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02215">
        <property type="entry name" value="phage_chp_gp8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="184" mass="21241" checksum="9CCC239E77023A6E" modified="2005-09-27" version="1">MLASSMLCIIRKTVPTSLPITLSEVKSFLRINNNQDDTLINNLITMASEYAQWYIEKSLMKQTWEISCSGYIPGKIQLLFNPIIKVNYVKITHTNGTEELIDQKYYYVDTIQSYISFNKQIHGNKIEIVYEAGYTDNASIPAQIKHGILHHVAVSYKNRESENINNLTFIKNIYSPFRELKLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS99</accession>
    <name>Q3YS99_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HesB/YadR/YfhF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304484.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0363"/>
    <dbReference type="EnsemblBacteria" id="AAZ68406">
        <property type="protein sequence ID" value="AAZ68406"/>
        <property type="gene ID" value="Ecaj_0363"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0363"/>
    <dbReference type="eggNOG" id="COG0316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069054_5_3_5"/>
    <dbReference type="OMA" id="CHGFQYT"/>
    <dbReference type="OrthoDB" id="1891602at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="FeS_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S_biosyn" evidence="2">
        <location>
            <begin position="9"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="151" mass="16650" checksum="D83EDEDF09BBF242" modified="2005-09-27" version="1">MSNLKSADLTLTENAVNKIKHLLDQEEDTGSLLCLSVSGGGCSGFQYNFNIGNINTLLADDEDDEDDDYDFLDEDDDEEYDDEDELEGKSSAKFTVFDEETGKPLLLIDKASNDLLKHSTIDYVEDINGSRFIVNNPIAKSKCGCGNSFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSA4</accession>
    <name>Q3YSA4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0358</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304479.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68401">
        <property type="protein sequence ID" value="AAZ68401"/>
        <property type="gene ID" value="Ecaj_0358"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0358"/>
    <dbReference type="HOGENOM" id="CLU_1127687_0_0_5"/>
    <dbReference type="OMA" id="CIPRSTK"/>
    <dbReference type="OrthoDB" id="2148403at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021387">
        <property type="entry name" value="DUF3023"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11224">
        <property type="entry name" value="DUF3023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="246" mass="27838" checksum="5DA761C36D17846C" modified="2005-09-27" version="1">MLGNQYLEPLIPCNKSLCEILLSLDIVTVTGCYKIGEANADGRVLIYIDKDGTKCFKSKKYEIFSGVGDSLFLLNFRIPTASVKNEKLSTLIGTSCMSKDYVTFNGYFFVKACRVRLFCKAIMDKVNRGGNRFYNGLLNRLNQPMNFPMSSIAMYGKFVLSRIVNGLWDPMFEEKLFLEYFSGLPVEFIVREKRKKCCCCCVRSRRRSDLSLEEYQSINQSDGNTVLSDVSISGVKTNSHSVGKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSB3</accession>
    <name>Q3YSB3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0349</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304470.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0349"/>
    <dbReference type="EnsemblBacteria" id="AAZ68392">
        <property type="protein sequence ID" value="AAZ68392"/>
        <property type="gene ID" value="Ecaj_0349"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0349"/>
    <dbReference type="eggNOG" id="COG5389">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2132517_0_0_5"/>
    <dbReference type="OMA" id="GVAINIQ"/>
    <dbReference type="OrthoDB" id="1918294at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007922">
        <property type="entry name" value="DUF721/UPF0232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05258">
        <property type="entry name" value="DciA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="108" mass="11984" checksum="FB5D3B68797B8FFD" modified="2005-09-27" version="1">MLFKSGPRSIGDAVEKLILNKCSKNDISKIEILLFLNWTVIVGKEISKIAQPKKLLFLDNVNTGVLYLVVNSGGVAVNIQYAIPIIVEKISVFFGFKVVHGIKIRQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-04-07" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSD8</accession>
    <name>Q3YSD8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA topoisomerase 1</fullName>
            <ecNumber evidence="10">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">topA</name>
        <name evidence="12" type="ordered locus">Ecaj_0323</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304445.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0323"/>
    <dbReference type="EnsemblBacteria" id="AAZ68367">
        <property type="protein sequence ID" value="AAZ68367"/>
        <property type="gene ID" value="Ecaj_0323"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0323"/>
    <dbReference type="eggNOG" id="COG0550">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1754">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002929_0_2_5"/>
    <dbReference type="OMA" id="PECKYTR"/>
    <dbReference type="OrthoDB" id="223233at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03363">
        <property type="entry name" value="TOPRIM_TopoIA_TopoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005733">
        <property type="entry name" value="TopoI_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025589">
        <property type="entry name" value="Toprim_C_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034149">
        <property type="entry name" value="TOPRIM_TopoI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42785">
        <property type="entry name" value="PTHR42785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13368">
        <property type="entry name" value="Toprim_C_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="zf-C4_Topoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="SSF56712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01051">
        <property type="entry name" value="topA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPOISOMERASE_I_PROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="11">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="10">
        <location>
            <begin position="167"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="10">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="820" mass="92602" checksum="FBC8891BD2CDBA23" modified="2005-09-27" version="1">MSLIIVESPSKAKTIGKYLGSKYKVVASFGHIRDFPAKSGSVNPDKDFAMTYEIISKSEKYVNKLIQVASKETHGIYLATDPDREGEAIAWHIVEVLKENNAIDDNVVINRMVFNEVTKNAILESIKHPRSINMDLVYAQQARRALDYLVGFTLSPLLWRKLPGSKSAGRVQSVALRLICDRESDIEKFVTQEYWDIEAKCQNVEGKQFSAFLSYYAGKKLEKFDIKNEEDAKRLAEEVKSRNYSVVKVEKKHTKRNPYPPFITSSLQQEASTKLGFTAKNTMLIAQKLYEGIDVGGEIVGLITYMRTDGFYIADEALDHIRSIIVSIFGKEYLPASARKYVKKVKNAQEAHEAIRPTNITITPDSLSNYLTPEQLKLYDLIWKRTVASQMNSAIIDQVVVELASSDKVVTLRATGSSLFFDGFYKVYGHNEDDDNKNNMLPLLQVSDECPLNEVIPHQHFTIPPARYNEASLVKKMEEIGIGRPSTYASIISVLQDRQYVILNQKKFFPTERGRIVNIFLVNFFSRYVEYDFTADLEEELDLISNGKINWKKVLHKFWFLFIDNVNSVKKLEFTEILKVISCEMENYIFSSDKSETGRVCPACNDGTLGLNISKNGIFLGCSGYPECKYTKNVGGDLVDDVDNVQYPKVLGIDTATCQEIYLKKGPYGVYLQIGSDKNGKRAAIPKNMNANLLDLQMAEKILNLPISLGTHPETCKEIKLGLGKFGAYILYEGKYFSIKNESNFLDVKLEDAIDIIANSSTNGVIRSLGISEDNKEVLLCKGRYGFYLKYDKLNVALKKGTDVEQMSLSDAMKLISEKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSE4</accession>
    <name>ISPE_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-diphosphocytidyl-2-C-methyl-D-erythritol kinase</fullName>
            <shortName evidence="1">CMK</shortName>
            <ecNumber evidence="1">2.7.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispE</name>
        <name type="ordered locus">Ecaj_0317</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.148"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. IspE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.148" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304439.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSE4"/>
    <dbReference type="SMR" id="Q3YSE4"/>
    <dbReference type="STRING" id="269484.Ecaj_0317"/>
    <dbReference type="EnsemblBacteria" id="AAZ68361">
        <property type="protein sequence ID" value="AAZ68361"/>
        <property type="gene ID" value="Ecaj_0317"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0317"/>
    <dbReference type="eggNOG" id="COG1947">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053057_1_0_5"/>
    <dbReference type="OMA" id="RWPSPAK"/>
    <dbReference type="OrthoDB" id="1938933at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00094"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050515">
        <property type="term" value="F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00061">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004424">
        <property type="entry name" value="IspE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527:SF2">
        <property type="entry name" value="PTHR43527:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010376">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00154">
        <property type="entry name" value="ispE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000235089" description="4-diphosphocytidyl-2-C-methyl-D-erythritol kinase">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00061"/>
        </source>
    </evidence>
    <sequence length="282" mass="31632" checksum="6C54B109B9636893" modified="2005-09-27" version="1">MSIFLVKAPAKVNLFLHITGKRIDQHHYLESLFVFVNVYDLLEVNIDGRKRGVYFSNFKISKYNNTVYKAIELLLKHSSVRPNISVNVIKNILVSAGLAGGSADAAAIIRLLGNMWNIDCKLLQDLALKVGSDVPACLESKTLFAKGVGEDILLLPDLLLPRYIILVAPRGKSLSTAKVFNHYKCDNFSASICDKLPVKQDDWMELIYNSKNDLLDVALNFVPEIEEILFVLKKLKNVFIARMTGSGATCFALFHELSHAENAAKHLRMTRPDWVIFNAKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSE5</accession>
    <name>Q3YSE5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="10">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ecaj_0316</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 10">Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:54452"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13887"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:23624"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13887"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the dus family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304438.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0316"/>
    <dbReference type="EnsemblBacteria" id="AAZ68360">
        <property type="protein sequence ID" value="AAZ68360"/>
        <property type="gene ID" value="Ecaj_0316"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0316"/>
    <dbReference type="eggNOG" id="COG0042">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013299_0_3_5"/>
    <dbReference type="OMA" id="RPWLFAD"/>
    <dbReference type="OrthoDB" id="1710586at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024036">
        <property type="entry name" value="tRNA-dHydroUridine_Synthase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004652">
        <property type="entry name" value="tRNA_dU_NifR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00737">
        <property type="entry name" value="nifR3_yhdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 10" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Dus" evidence="12">
        <location>
            <begin position="13"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ68360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="317" mass="35161" checksum="315799C97EF240D4" modified="2005-09-27" version="1">MDIRQFISEHPVILAPMSGVTDFPFRCLVKNIGAGLLVSEMIASRAMIIQTKQSLQKSQIQANTAVQLAGCDPAIMAEAAKLNEDMGAKIIDINFGCPVKKVVNSYAGSALMKDEITAAKILESVVKAVKIPVTLKMRTGWDNNNLNSPKLAKIAEESGIKMITIHGRTRTQMFNGKADWKFISNIKKCVKIPVIANGDIKNLDDIKTSLEESQADGVMIGRGAYGKPWFINQALHFIKSGIILPEPSPSEKLQIITNHYDHMIEYYGKDIGVKISKKHISWYSASLDNSAEFRSQVNRSNDNSFIRDKIIDFFSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSE6</accession>
    <name>Q3YSE6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor RpoD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Sigma-70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpoD</name>
        <name evidence="10" type="ordered locus">Ecaj_0315</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts transiently with the RNA polymerase catalytic core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the sigma-70 factor family. RpoD/SigA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304437.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0315"/>
    <dbReference type="EnsemblBacteria" id="AAZ68359">
        <property type="protein sequence ID" value="AAZ68359"/>
        <property type="gene ID" value="Ecaj_0315"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0315"/>
    <dbReference type="eggNOG" id="COG0568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_7_1_5"/>
    <dbReference type="OMA" id="HDSIWEM"/>
    <dbReference type="OrthoDB" id="1565734at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00963">
        <property type="entry name" value="Sigma70_RpoD_SigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009042">
        <property type="entry name" value="RNA_pol_sigma70_r1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007631">
        <property type="entry name" value="RNA_pol_sigma_70_non-ess"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007127">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042189">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012760">
        <property type="entry name" value="RNA_pol_sigma_RpoD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028630">
        <property type="entry name" value="Sigma70_RpoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04546">
        <property type="entry name" value="Sigma70_ner"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03979">
        <property type="entry name" value="Sigma70_r1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00140">
        <property type="entry name" value="Sigma70_r1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02393">
        <property type="entry name" value="RpoD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="8">
        <location>
            <begin position="413"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_sigma70" evidence="9">
        <location>
            <begin position="583"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="6">
        <location>
            <begin position="584"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-2" evidence="6">
        <location>
            <begin position="389"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-3" evidence="6">
        <location>
            <begin position="468"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Interaction with polymerase core subunit RpoC" evidence="6">
        <location>
            <begin position="413"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="622" mass="71066" checksum="6375F0CC1CBA40B0" modified="2005-09-27" version="1">MKDLQTDKELLKTLVSKGLKQGFVTFNDINDVFSDYVLSADNIDETISMLQDSGINVLEQSDEDEANSIVEENRIKEDLDEDDVSDGGIKSWDFGQTDDPIRMYLCEMSSVELLSREGEIEIAKKIKSEKVNMLRSLVESPVVLRTFMSWRDDLVNEQIMLRDLIDLDANYRYEFPEKFADADEASVLDYDKDLIDESDEDTDISEDDDEENVSINASILEMENALLPKVVSILDSVIASAEKILELKKQYQGKINQDIEKQYNDLHDSIWEMIYQIKLSNSAVLSITQQIYSLSKSIAAEEAKIISLAESYGIQRKDFLDAYNTNSVLQKKGTSPQWNNLLANEESNIVSMYSKIKLLSGENNLTEFKALVTKIQKHERAANQAKQEMIKANLRLVVSIAKKYSNRGLQFLDLVQEGNIGLMKAVDKFDYKRGYKFSTYATWWVRQAITRAIADQARTIRIPVHMIETVNKINRTLRQMLHEMGREPTLEELSAKLNINVDKIRKVMKIVKDPVSLESPIGDDDSSTFGDCIEDKRAVKPEDAAVLADLREITTKVLSTLTPKEERILRMRFGIGKGGKDHTLEEVGKLFNVTRERIRQIEAKALRKLRHPSRARKLRGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSF1</accession>
    <name>Q3YSF1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribonucleoside-diphosphate reductase subunit beta</fullName>
            <ecNumber evidence="3">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 5">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="3 5">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304432.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0310"/>
    <dbReference type="EnsemblBacteria" id="AAZ68354">
        <property type="protein sequence ID" value="AAZ68354"/>
        <property type="gene ID" value="Ecaj_0310"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0310"/>
    <dbReference type="eggNOG" id="COG0208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035339_1_2_5"/>
    <dbReference type="OMA" id="KVGEYQR"/>
    <dbReference type="OrthoDB" id="1384440at2"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="PTHR23409"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000355">
        <property type="entry name" value="NrdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000355-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000355-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="324" mass="38270" checksum="A57CB3FBA0308C22" modified="2005-09-27" version="1">MSLLSSRPIYKPFNYPWAYDAWLCQQRIHWLPEEVPLADDVQDWKTVLSEKEKNLLVQIFRFFTQADIEVNNCYMRHYSNIFKPTEVCMMLAAFSNMETIHIAAYSYLLDTIGMPEIEYQAFLKFEVMKKKYDYMQKFEECRKNDKRHVAKTLAVFGAFTEGLQLFASFAILLNFQRFGKMKGMGQIIAWSVRDETLHTESIIRLFNTFVQENYEIWDDSLKHELYEACKIIVSLEDEFINLAFACGDVEGLSAKEVKKYIRYIANRRLLQLNLEPIYDANKNPLLWLDEILNGVEHTNFFENRVTEYARAATEGTWEEAFEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSF2</accession>
    <name>Q3YSF2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304431.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68353">
        <property type="protein sequence ID" value="AAZ68353"/>
        <property type="gene ID" value="Ecaj_0309"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0309"/>
    <dbReference type="HOGENOM" id="CLU_017350_0_0_5"/>
    <dbReference type="OMA" id="MPHNERE"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="840" mass="95168" checksum="AC0406A552980E00" modified="2005-09-27" version="1">MLTRDQQREAIDKRKLKQYIKDTVSEKIDDPLLDMTNITPLCVTLLLDVEPDFTLFKEKVIDILKEYNDLIAIHYLPKGLTIVDDRTSEMSWNSVKELHKLYHNHQLSVASNDGSSILTRQKFNLSPNKQDIIIDNNLKYRIQYYLLSSLIDQCFDTETIKTYSEEALFLTDNTRKNIIQKLGEILHHHVQKKTFSSYAKYLKNLYEIQELYFTEKGYDLLSTTLQHITLRKLNITQQNMSYTFSPLLDNGCDININCNIPEYVDIFLETSDITISGTVYGNIFSKYGSITITNAKEDCSKNVVSIFGVVSINGKIINQKLPNELIINSNFCTNIKRSSIITRQTNLCQTPQTISSFSSEDFINNQHTNNIDALKIELFNAKLQYYAIIASIEWPTKQPTYKNAELLFQGKIQYTDIIDKLCNINQNTLPNIRIDHSINLPFHNVFIKDKKLIIEKLNKFIGVYLTTRTNNGKDFIKKFHIDEIAIHSQTEYAQLILYNTNITIFGKFQGLIHTHTGNITVIGEIKKSRLTTNTGLIRIKPSNSNANTTIASDSLIIAKYGTIEITGKHNYCYINGNKFITNPDETSSATTSLNKTHPQTSTKAITSQNSTKHAIPFSKNTIHYLNHVNIIDNIEAENRLPITFFDYGATTFIHDVHIKDKKLILRNLNNNIALHLTNITSNGTNLKSKFGVNEVVIYSDAIEHAYLIFHDTDVTIIGSFFGILHTNRGNIKINGEIKKLSKISTNAGSIEITGKVNKAEITANTGSIEIKPHSNDLTTEVAINSPLTAKYGTVKITGIHSRCRIDGNHVITTQPSSCIPHSCLQKLSVILPYLADPLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSF5</accession>
    <name>Q3YSF5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamate dehydrogenase</fullName>
            <ecNumber evidence="2">1.4.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0306</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamate + NAD(+) = 2-oxoglutarate + H(+) + NADH + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.2"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.4.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304428.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0306"/>
    <dbReference type="EnsemblBacteria" id="AAZ68350">
        <property type="protein sequence ID" value="AAZ68350"/>
        <property type="gene ID" value="Ecaj_0306"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0306"/>
    <dbReference type="eggNOG" id="COG2902">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003404_1_1_5"/>
    <dbReference type="OMA" id="RATRWFL"/>
    <dbReference type="OrthoDB" id="79339at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019551">
        <property type="term" value="P:glutamate catabolic process to 2-oxoglutarate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028971">
        <property type="entry name" value="NAD-GDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007780">
        <property type="entry name" value="NAD_Glu_DH_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43403">
        <property type="entry name" value="PTHR43403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05088">
        <property type="entry name" value="Bac_GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036761">
        <property type="entry name" value="GDH_Mll4104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1582" mass="182783" checksum="F32A40CB47C5BD8C" modified="2005-09-27" version="1">MYKNFNQKIISVLELIEEDDDHLLKSFIKEFYSSSYDNDITLDNKFFLFIARDLYELVKMKQHNESKVKVFNTEQTDNDITKNVTIVEIINDNLPFIIDSIIITIKKHNAFIYHYTNAVLNIERKDKHIHTISSAQSCSNDETRESIVYFIISHIDEGSHKSLKHEIEKNLKLVSQCVNDWKLMLQHFDSALNAMKSFHANSIEEEITFLEWLRKDNFIFLGHEEYIVDQNKLSINNNSSLGLQKINTSDELSKDLPKSKQHIYIIQSNILSTVHRHEYMICIGLRIFNKDNILIKEHRFYGFFASIISFQDANAIPLIRSKIKAVEKKSGFTKGGHSNKALVDILQRFSRDELFQFSEEELFEISIGILSLANNPKIKLFIIKDQNHSFVNCIIFVPKALASTELANKMSYILEQMLTGKVVNNQYNMLNEYNLVRLQFILKAQDKLFSNFSESEIEAKLIAASRRWEDKLQDVMRCNLGSINPFLQYLTAFPPSYQEYFNPKSAYHDITKLEQVCKYNTSEADLYLIKNSVHYQLKIYIPQESNLQLSKILNIVKKMGTNIILHHSYTITAKITIYLHHFILSNTKQSFDHNNIKQQFETTIKKIFAKEIENDYFNSLVILANLQWKEVMLIRALSRYLKQVSFNYSQIYTQKVAIKYPKILLNFIKLFNARFNPTLANNEESSAIQSKINELLSEITDVVHDHILRSIYALILAILRTNYYQGKDYLSIKLDSSKVPNIPLPCPFREIYVYSNLFEGIHLRGGKVARGGIRWSDRTEDFRTEILGLMKAQMTKNSVIVPVGSKGGFILKRPPKNGALLKSTAVECYKNFLRGILDITDNMIDDKYVTPDNIIKYDEYDPYLVVAADKGTATFSDYANEISEEYNFWLGDAFASGGSIGFDHKKMGITAKGAWVAAQRHFWLMDKDIRNEPFTVIGIGDMSGDVFGNGMLLSDKIHLIGAFNHNHIFIDPSPDPTKSFLERKRLFNLPNSSWEDYDKSCISKGGKVFSRNSKILDLTPEIKELFNIEEDQVSPNTLIRHLLKAEVDMIWNGGIGTYIKSSQETNAEVSDKANDALRINGSEVKAAMIIEGGNLGVTQTGRVEYAHSGGKINTDFTDNSGGVICSDFEINIKICLRLAMKNNYISLAERNKILDDIMDAVPSIVLESHNKLETKALMLECIQAQNRIEQHHRLLKYLEKIQVLNRDIEFLPSDEEITKMSAGMKGFTCPQIAILIAYTRTFIKNEVMLSNIFHHSSHLLNLYESQYLLSYFPQYIRDNFSQYIRQHPLKKEILATCIVNDIVNRMGCIFVNHIIENIGITVEDVIKIYVITTHIYNLYEIWKTLDELDSKIHINIYTSLIREVQKFIGQITFWFLRNSCKFTNIDTKLEELSSQISLIEENIESILCNESLEIYNNTLSSLMEYNINKSIAKKITKLKFLTISLDIIHLTNSSNLPLLTIGKIYFQLRSLLNFSRIRDFAAHMEHNSSYWQRIAIRNLLDELNDYQSTITENILKQAEPTLSLAIKLDKVDSIINDIIQEWYIKNQEKLNRYYNFLNEINTTQLDLSKLMLIIKSLDMFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSF6</accession>
    <name>Q3YSF6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0305</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304427.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0305"/>
    <dbReference type="EnsemblBacteria" id="AAZ68349">
        <property type="protein sequence ID" value="AAZ68349"/>
        <property type="gene ID" value="Ecaj_0305"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0305"/>
    <dbReference type="eggNOG" id="COG1826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166594_0_0_5"/>
    <dbReference type="OMA" id="YKECNTE"/>
    <dbReference type="OrthoDB" id="2073777at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="88" mass="10021" checksum="9EA292E1C30A35EF" modified="2005-09-27" version="1">MLGVGISEVIVIILVACLVIDPKKLPLVTKSLGFYYKKFVEVKGEILNYFNKVYNDCTEEQNNNVVIKEIIGDDGKTYKSYEVKKDNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSG2</accession>
    <name>PSD_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylserine decarboxylase proenzyme</fullName>
            <ecNumber evidence="1">4.1.1.65</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="1">Phosphatidylserine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Phosphatidylserine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">psd</name>
        <name type="ordered locus">Ecaj_0296</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:20828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="4.1.1.65"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="1">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylserine decarboxylase family. PSD-A subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304421.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSG2"/>
    <dbReference type="STRING" id="269484.Ecaj_0296"/>
    <dbReference type="EnsemblBacteria" id="AAZ68343">
        <property type="protein sequence ID" value="AAZ68343"/>
        <property type="gene ID" value="Ecaj_0296"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0296"/>
    <dbReference type="eggNOG" id="COG0688">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072492_0_0_5"/>
    <dbReference type="OMA" id="VSIFMSP"/>
    <dbReference type="OrthoDB" id="891720at2"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00616"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00664">
        <property type="entry name" value="PS_decarb_PSD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033175">
        <property type="entry name" value="PSD-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35809">
        <property type="entry name" value="PTHR35809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00164">
        <property type="entry name" value="PS_decarb_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_0000262209" description="Phosphatidylserine decarboxylase beta chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000262210" description="Phosphatidylserine decarboxylase alpha chain" evidence="1">
        <location>
            <begin position="184"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="1">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autocatalysis" evidence="1">
        <location>
            <begin position="183"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="1">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00664"/>
        </source>
    </evidence>
    <sequence length="226" mass="25005" checksum="5BC5F53776940859" modified="2005-09-27" version="1">MLFNLIHNIHKEGYIFIMISFLASCIGFAISCSLGIVCLAMSLLCIFFFRDPIRVVPEGDNLITSPADGLILDIKEVNSPIDSSTQVVCISIFLNILNVHVNRIPVSGTIKSTEYIPGRFISASLDKSSELNERQRLIIESNLDKHLIILDQIAGLIARRIVCNAYQGQNVNLGEKFGIIRFGSRVNIYLPLSTHISVFKGQTVIAGETILAYLKEAPKQLTVKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSG4</accession>
    <name>Q3YSG4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sodium:alanine symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0294</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304419.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0294"/>
    <dbReference type="EnsemblBacteria" id="AAZ68341">
        <property type="protein sequence ID" value="AAZ68341"/>
        <property type="gene ID" value="Ecaj_0294"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0294"/>
    <dbReference type="eggNOG" id="COG1115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024867_1_1_5"/>
    <dbReference type="OMA" id="GMATKFV"/>
    <dbReference type="OrthoDB" id="883596at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015655">
        <property type="term" value="F:alanine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001463">
        <property type="entry name" value="Na/Ala_symport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30330">
        <property type="entry name" value="PTHR30330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01235">
        <property type="entry name" value="Na_Ala_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00175">
        <property type="entry name" value="NAALASMPORT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00835">
        <property type="entry name" value="agcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="460" mass="50263" checksum="8608B6768C6345C5" modified="2005-09-27" version="1">MTTLELTDFFPYVSRTLESIFSFTIFRIPLIILWVIYLGIFCTLKFKFVNIRLFKHALQVLVKPQNKKDNNTISNIQAFITAISGTIGLGTISGVVIAISTGGPGAVLWMIIIGILGMSVKFAEVTLGFKYYLIEKKNNIGGPFQYIRCGLVELGLPKTGKIVAYIYAVTMIIAMSFGGISFQSNQATVLLSNTFHQVNSSVIVLILTLLSGLVIIGGITRIAKIMTKLSPIMVVLYITICTAVIISNSDQLFSTFNIMIHGMLEGNSMTGGAIGSLIAGVRRSIFANEAGVGTSPIIHSSTNETEPARVGCIAMLEPFIDTVLVSFITGIVVILTGSYSTISSSHNVITINNIFSNICPICQYMIFPFIIFAFSFSSIITYSYYCEISWKYILGNSCHVGLCRTLIMCIIIISGLSKNIIPISQLGDLLFIFLTIPNGITIILLSNIINQELKTYLSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSG7</accession>
    <name>Q3YSG7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NusB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0291"/>
    <dbReference type="EnsemblBacteria" id="AAZ68338">
        <property type="protein sequence ID" value="AAZ68338"/>
        <property type="gene ID" value="Ecaj_0291"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0291"/>
    <dbReference type="eggNOG" id="COG0781">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087843_4_2_5"/>
    <dbReference type="OMA" id="YVNIASH"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="NusB" evidence="2">
        <location>
            <begin position="10"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="148" mass="17059" checksum="B85D79E3FAF68B31" modified="2005-09-27" version="1">MIFNNYTINDIDNLIDYLYEMKEVLDLIQIDNQLLIKIMNTMLLTFNTINSTIATFLNEKWSIDRINLISLSIIKTAVCELICSNTDAVIIINEYVNIASHILEKTEVNFINAILNQVKLTARLTFDNDINNFHHVEQSENSAVFNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSG8</accession>
    <name>Q3YSG8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Excinuclease ABC subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ecaj_0290</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304415.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0290"/>
    <dbReference type="EnsemblBacteria" id="AAZ68337">
        <property type="protein sequence ID" value="AAZ68337"/>
        <property type="gene ID" value="Ecaj_0290"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0290"/>
    <dbReference type="eggNOG" id="COG0178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001370_0_0_5"/>
    <dbReference type="OMA" id="PFEGIIP"/>
    <dbReference type="OrthoDB" id="152379at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004602">
        <property type="entry name" value="UvrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041552">
        <property type="entry name" value="UvrA_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041102">
        <property type="entry name" value="UvrA_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17755">
        <property type="entry name" value="UvrA_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17760">
        <property type="entry name" value="UvrA_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00630">
        <property type="entry name" value="uvra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0228">DNA excision</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="612"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="649"/>
            <end position="656"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAZ68337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="950" mass="106051" checksum="526DC0FBA45A0D33" modified="2005-09-27" version="1">MANFISIRNAKEHNLRNINIDLPKNKLIVITGVSGSGKSSLAFDTIYAEGQRRYVESLSSYARQFLDLCTKPDVESITGLSPTIAISQKFSSKNSRSTVSTITEIYDYLRIMYSNIGIPYSPTTGLPITKQSLAQIVETIFKLPLGTKISILGILIRGKKGEHIKEIYDLKKQHYKLLKINNITYDINNIPSLDRNKCHDIHVVVNQILVSEDSIEKITENIKTALNIGNGIIQIEILDLPEECKSETYHKNQILVFSENFACPETGFSIEEIEPRLFSFNTSYGSCKACSGIGKKYNIDQNLVINDNLSILEGAIHPIGPIDIKVLHHKAKSQFSYYYASIILSLSKYYNVDLTKPWKELDKNIKDIILFGSKTQTSTNYQKNKPFEGIMNILNNKEDIAIEKLAEQYLSINTCSECQGFRLRPEALSVKIDNKHIGEFTKLSVVEAISWCKALPSKLNEKQKHIAYKLLEEIIKRLTFLKNVGLGYLTLDRESATLSGGESQRIKLASQIGSGLTGIIYVLDEPSIGLHQRDNTLLINTLKTLRDIGNTVIVVEHDEETITNADYVVDIGPEAGQNGGYIIAHGTPKEIMENNNSITGQYLSNKKTIPNFKKQKTFNKWIEIIGAHENNLQNINVKIPLNAFTCITGVSGSGKSSLINDTLYKYAINKLNNANCSYGKCISISGLEHIDKVIEIDQTPIGRTPLSNSATYTGLFLHIRSWFAGLPLSKARGYTISRFSFNTKGGRCEACKGDGYVKIEMHFLPDVHVKCEQCKGLRYNKETLEVTHQGKSIADVLNMTVDQAHEFFIDIPLIKEKLDSLRSVGMGYIKIGQTSNTLSGGEAQRIKLSRELSKRSTGKTLYILDEPTTGLHFADIDNLLHVLHKLRDMGNTIIVIEHNLHVIKTAEYIIDIGPNGGEDGGRVVASGTSQEIAKNPNSITGQYLKHYLNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSI4</accession>
    <name>Q3YSI4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone-like bacterial DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0274</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304399.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0274"/>
    <dbReference type="EnsemblBacteria" id="AAZ68321">
        <property type="protein sequence ID" value="AAZ68321"/>
        <property type="gene ID" value="Ecaj_0274"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0274"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_3_3_5"/>
    <dbReference type="OMA" id="MFMEMIK"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="94" mass="10588" checksum="6BC1C08DDD51F0C1" modified="2005-09-27" version="1">MSKDMVVRTLMGELGLTKKVSSQAYDLVMLSIQNALENSHLIRLHNVGTLHVIQCAEKKYHNPKTGKLETLAPKKRVRFRSSKKLSNAINSLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSP1</accession>
    <name>Q3YSP1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0215</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0215"/>
    <dbReference type="EnsemblBacteria" id="AAZ68264">
        <property type="protein sequence ID" value="AAZ68264"/>
        <property type="gene ID" value="Ecaj_0215"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0215"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040413_0_0_5"/>
    <dbReference type="OMA" id="NGIGIMQ"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="241"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="470" mass="53402" checksum="4E585D9E17038168" modified="2005-09-27" version="1">MKYFRVLLLVFLVITLFFAVGYTHIYLKDDYLYEVSKEINVNIKSVLVNSVINKYAQLLAKAPSHVATNTYYMNLLIKLRAEFLKALGEIKGFYVILYNVDGNIIFSNRNSDDIADQDLLLHSEDMASLLKGEYVDHKVNNSAITESIFPIFPLDNKESHPLMFLKIIKNSGNLSSFIVNFYTIFLIIVILVIIIFVVVTLCIYYKNTKILSKQYDANLKLKEAKEVAEQENISKSQFLANVSHELRTPLNSIIGFSEMMKSESMGAIDNVHYKEYINDIHNSGVHLLSLINDILDFSKVEANKLVVEFVKFDLSKIISSCFNMMVPRAEQAKVKLEKVLPSNKVIMVADPKRMKQVIINLLSNAIKFTPENGVVKLVVEYNIEEKLIDIEVIDNGIGIAQQDLYKVMSIFGQVDSRHARKYEGTGLGLPLSKKLVELMDGVFKIKSEPNLGTIVTLTFPYTDTLEEKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSP3</accession>
    <name>Q3YSP3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304340.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68262">
        <property type="protein sequence ID" value="AAZ68262"/>
        <property type="gene ID" value="Ecaj_0213"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0213"/>
    <dbReference type="HOGENOM" id="CLU_067294_0_0_5"/>
    <dbReference type="OMA" id="CACHISE"/>
    <dbReference type="OrthoDB" id="1506117at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="328" mass="35970" checksum="5E19AB85BFAEE739" modified="2005-09-27" version="1">MVLQNDNITDDTQNNKDEVHDTQQSTEIVKESSVVTSETQPTHDKKLDKIDFNTLFLAISFAGLLIEAASAIFNLVSTYVYVPERIKHIVATAFYAISIIVSLSMMASSILAIKQSLNSKKKLQETSTGPNKEAERSINEGLLGYDKLKQKQANIQISENTITIISEILWIIVSAASLVMITVGTGTPALELASLCLAVIAPFLAFISCALRLSDANISRKTATSNKEKRHASNFTVLCTIILLFEAIHCGCHVAEAVMLGGKMQNIYDFQDAIVLGLELAAVVMFIAAFFIEKYLDKKAEKSDPQATPSSLLDDKAIDRMFREAQIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSQ0</accession>
    <name>FMT_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionyl-tRNA formyltransferase</fullName>
            <ecNumber evidence="1">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fmt</name>
        <name type="ordered locus">Ecaj_0205</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-tRNA(fMet)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304333.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSQ0"/>
    <dbReference type="SMR" id="Q3YSQ0"/>
    <dbReference type="STRING" id="269484.Ecaj_0205"/>
    <dbReference type="EnsemblBacteria" id="AAZ68255">
        <property type="protein sequence ID" value="AAZ68255"/>
        <property type="gene ID" value="Ecaj_0205"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0205"/>
    <dbReference type="eggNOG" id="COG0223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033347_1_1_5"/>
    <dbReference type="OMA" id="CCPVVAY"/>
    <dbReference type="OrthoDB" id="2009156at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08704">
        <property type="entry name" value="Met_tRNA_FMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00182">
        <property type="entry name" value="Formyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044135">
        <property type="entry name" value="Met-tRNA-FMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000020058" description="Methionyl-tRNA formyltransferase">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="111"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00182"/>
        </source>
    </evidence>
    <sequence length="303" mass="33730" checksum="FA577524A06CBC86" modified="2005-09-27" version="1">MKIIFMGSPEFSVSALSYLLENESHEVVAVYTKIPKPAGRRGRILTKTPVHIIAEQNNIEVNTPKSLKHDAEQEKILSLNPDVIVVVAYGLIIPQGVLSIPKYGCINIHPSLLPRWRGAAPIHYAILSGDDKTGVTIIQMNELLDEGDILLQRDIPIDEQDNIDTLSKKLAHLGSSMLIEVLDNIDNLVPIKQNNDDATYAHKIIDFHIDFNEAADVICRKVRALYPRAFLLFNGKRLRILKTSYYSDSSVQDLKPGAVIDSHMNIKCKDGVLVPLVVQMEGKNPCNIDDFILGYNVLNCSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSQ1</accession>
    <name>Q3YSQ1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Lon protease</fullName>
            <ecNumber evidence="7 8">3.4.21.53</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-dependent protease La</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lon</name>
        <name evidence="15" type="ordered locus">Ecaj_0204</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8 11">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="7">By heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8 11 12">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="7 8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304332.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0204"/>
    <dbReference type="MEROPS" id="S16.001"/>
    <dbReference type="EnsemblBacteria" id="AAZ68254">
        <property type="protein sequence ID" value="AAZ68254"/>
        <property type="gene ID" value="Ecaj_0204"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0204"/>
    <dbReference type="eggNOG" id="COG0466">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004109_4_3_5"/>
    <dbReference type="OMA" id="GAWQVVD"/>
    <dbReference type="OrthoDB" id="128102at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01973">
        <property type="entry name" value="lon_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027543">
        <property type="entry name" value="Lon_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001174">
        <property type="entry name" value="Lon_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 7 8" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 7" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="14">
        <location>
            <begin position="7"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="13">
        <location>
            <begin position="593"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="active site" evidence="7 9 11">
        <location>
            <position position="680"/>
        </location>
    </feature>
    <feature type="active site" evidence="7 9 11">
        <location>
            <position position="723"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <begin position="357"/>
            <end position="364"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR001174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAZ68254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="801" mass="89902" checksum="4A528756CF373509" modified="2005-09-27" version="1">MKNKALLPVLTLRDTIVFPQVIIPLFVGRERSINALEYAAQHNNCKILLLTQIDGSVDNPTADDLYKVGTVAEIVQLLRLPDGAVKILIKGENRAKVIDIVEDNMFFKANVSVVYESDLVIDDKLEALKRSVLSEFDSWNKLSKKIQAEAAASIYDMKELGNLADVIASHLSIKISDKQQVLETFNVTKRLEKIYDFLKLEISVLNVQKKIRNRVKSQFETTQRVYYLNEQLKAIQKELEESDSSSVDIDSASEFEKRINSTPLSDEAKERLTADLKRYKKMNFMSPEANIISSYLHWVLDLPWGKYSNAKINMNNSIKILDNNHYGMHKIKERILEFLAVLKRLKKPKGPILCLVGPPGVGKTSLARSIAEATGRNFIHMSLGGIHDESEIRGHRRTYVGAMPGKIIKEMKKAKTCNPLFLLDEIDKIGSDFRGDPTAALLEVLDPEHNKHFVDHYIEVEFDLSNVMFIATANTLNLSKPLIDRMEIINISGYTENEKLEIAKAHLIPKLYKDHGLQEKEWSISDDAIYHLIRMYTRESGVRNFKREIANLMRKAVKEILINKEVKSISIDMDNIEKYAGVRKFTFGMIEKENLVGMVTGLAYTDSGGDILTIESVLMSGKGEVKYTGKLGEIMQESVKAAYSYVRSRCLSFGINPKKFLENDIHVHVPEGAVPKDGPSAGVAMCTSIVSLMTNIPVKNTVAMTGEVTLRGRVLPIGGLREKLLAALRSGIKTVILPSRNEKDIVEIPDNIKEGLELVFVSSVDEVISAALANQVVDIVKQDHIVAQDMVEIDDLDAIQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSR9</accession>
    <name>Q3YSR9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EndoU_bacteria domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0185</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304314.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68236">
        <property type="protein sequence ID" value="AAZ68236"/>
        <property type="gene ID" value="Ecaj_0185"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0185"/>
    <dbReference type="eggNOG" id="ENOG502ZA3K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089587_0_0_5"/>
    <dbReference type="OMA" id="RLGGMHF"/>
    <dbReference type="OrthoDB" id="1355433at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037227">
        <property type="entry name" value="EndoU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029501">
        <property type="entry name" value="EndoU_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14436">
        <property type="entry name" value="EndoU_bacteria"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142877">
        <property type="entry name" value="SSF142877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230981" description="EndoU_bacteria domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="EndoU_bacteria" evidence="3">
        <location>
            <begin position="126"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="271" mass="31307" checksum="802974B60E88DA1F" modified="2005-09-27" version="1">MKFYKFIYFCFLLLPLNLIACQPVETPDQCTHNPSGINPFFKAGKYDKCDDLPVAPMLNKFDKAVLDICGDWGSTPKREDFKNLLDKKEYKNYIDEIYEGLDRQVFTPDADLDTFKKELTELWFNNHGFTHVMCGQPRRGRLGGMHFFGRYLQAQKNQWVGRYYDDDLMDEISDKVFLVGVAFKNSRGQLTVDPKKSYDLLHANEIILHATRAYKGLSKNSQLVEKANGRCIYDNEDVNYVFVARKNSIVTFFAAFNPTCTRGQTECACVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSS9</accession>
    <name>Q3YSS9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0175</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304304.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0175"/>
    <dbReference type="EnsemblBacteria" id="AAZ68226">
        <property type="protein sequence ID" value="AAZ68226"/>
        <property type="gene ID" value="Ecaj_0175"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0175"/>
    <dbReference type="eggNOG" id="COG1863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086615_2_2_5"/>
    <dbReference type="OMA" id="PYFLWAM"/>
    <dbReference type="OrthoDB" id="1987527at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="PTHR34584"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="158" mass="18071" checksum="90F1C09CEC61102F" modified="2005-09-27" version="1">MKKFFILFIFWLTLSGYFDLFFITLGAVSCITTLLITRILKNAIPSNENYIYHQSRTQILHFLSYFSWLIQQVILSNIHIIKKVWNFRTRINPSIFKVIQTKQTTGLNALIVANSITFTPGTVSIDVTDTTPYKITVLAIDEESMSGVVDIDNKATNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YST1</accession>
    <name>RNH2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease HII</fullName>
            <shortName evidence="1">RNase HII</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhB</name>
        <name type="ordered locus">Ecaj_0173</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase HII family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304302.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YST1"/>
    <dbReference type="SMR" id="Q3YST1"/>
    <dbReference type="STRING" id="269484.Ecaj_0173"/>
    <dbReference type="EnsemblBacteria" id="AAZ68224">
        <property type="protein sequence ID" value="AAZ68224"/>
        <property type="gene ID" value="Ecaj_0173"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0173"/>
    <dbReference type="eggNOG" id="COG0164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036532_3_2_5"/>
    <dbReference type="OMA" id="YPTKLHL"/>
    <dbReference type="OrthoDB" id="1685277at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07182">
        <property type="entry name" value="RNase_HII_bacteria_HII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_B">
        <property type="entry name" value="RNase_HII_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022898">
        <property type="entry name" value="RNase_HII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="PTHR10954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF18">
        <property type="entry name" value="PTHR10954:SF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000235721" description="Ribonuclease HII">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-2" evidence="2">
        <location>
            <begin position="19"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <sequence length="209" mass="23593" checksum="25AA7B384994B17C" modified="2005-09-27" version="1">MPDFSIENEISKSINKKNCIIVGVDEVGYGSLAGPVVSAASFFLRHDNEIIYNIKDSKKLTPKKRIEVYNTITSIVKWSIGFADVYEIDQHNILNATHLAMQRALNGLNCDIDYVIVDGNKVPDLPWNRKAIVDGDNISVSIAAASIIAKVTRDKLMETLHTQYPEYQWNKNKGYGTKHHLESLYKYGKTIHHRNTFAPLPGITKLYSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YST7</accession>
    <name>RL10_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplJ</name>
        <name type="ordered locus">Ecaj_0167</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. The N-terminus interacts with L11 and the large rRNA to form the base of the stalk. The C-terminus forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304296.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YST7"/>
    <dbReference type="SMR" id="Q3YST7"/>
    <dbReference type="STRING" id="269484.Ecaj_0167"/>
    <dbReference type="EnsemblBacteria" id="AAZ68218">
        <property type="protein sequence ID" value="AAZ68218"/>
        <property type="gene ID" value="Ecaj_0167"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0167"/>
    <dbReference type="eggNOG" id="COG0244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092227_0_0_5"/>
    <dbReference type="OMA" id="KMSVICA"/>
    <dbReference type="OrthoDB" id="1470978at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05797">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00362">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022973">
        <property type="entry name" value="Ribosomal_L10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="PTHR11560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000120960" description="50S ribosomal protein L10">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00362"/>
        </source>
    </evidence>
    <sequence length="160" mass="18233" checksum="552E89A9DC3B6A69" modified="2005-09-27" version="1">MKRSDKELHLSKVEGLFSKFKYFIIANFQGMVANDFFSLRKELKMANSGLMVVKNSLSRIALKKMGREELSAKFFGSIFIVYSDDIILISKILAKFMKDNKSKISLLCAYDSNEILDSEKVLYFASLPSLRELHAQIMSMISYNIPVRLALCLKALGNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSV7</accession>
    <name>Q3YSV7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="2">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="2">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">sucD</name>
        <name evidence="7" type="ordered locus">Ecaj_0147</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304276.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0147"/>
    <dbReference type="EnsemblBacteria" id="AAZ68198">
        <property type="protein sequence ID" value="AAZ68198"/>
        <property type="gene ID" value="Ecaj_0147"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0147"/>
    <dbReference type="eggNOG" id="COG0074">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_5"/>
    <dbReference type="OMA" id="VIICITE"/>
    <dbReference type="OrthoDB" id="1081709at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="1 2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA_binding" evidence="6">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="2">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="2">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="2 3">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="295" mass="30604" checksum="E9D4AF0B241FA406" modified="2005-09-27" version="1">MSVLVDRNTKVICQGFTGAHGTFHSEQAIAYGTNMVGGVTPGKGGTSHLNLPVFNTVKQAKLDTGANATVIYVPAPYAADAILEAIEAGIELIICITEGIPILDMVQVKRALLSSNSILIGPNCPGIITPEECKIGIMPGYIHKRGHIGVISRSGTLTYEAVAQTTEVGLGQSTCIGIGGDPIHGMSFTDCIKLFINDPDTHGIIMIGEIGGSEEEEAAEFIKLSGTDKPVVGFIAGKTAPPGKRMGHAGAISSGTSGSAESKIEAMKNAGIVIAETPAVIGKTMLEAMNNKYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSW1</accession>
    <name>Q3YSW1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304272.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0143"/>
    <dbReference type="EnsemblBacteria" id="AAZ68194">
        <property type="protein sequence ID" value="AAZ68194"/>
        <property type="gene ID" value="Ecaj_0143"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0143"/>
    <dbReference type="eggNOG" id="COG1235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044538_2_1_5"/>
    <dbReference type="OMA" id="CGPDFRQ"/>
    <dbReference type="OrthoDB" id="1712770at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="34"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="264" mass="29823" checksum="1920F2C449C93C63" modified="2005-09-27" version="1">MKVTILGCGSSSGVPVVGCRCDTCSSSLKYNKRMRSSILVESADVQLLVDTTPDLRFQALQNNLSSVDAVLYTHFHADHCDGIADLQQFLPKHDVNNIPIYSDITTLCLLTASNSYFFIPSGHASVSKKCSYLKANVIYYYKEFVVKDFHILAIKQIHGVNSSNGFIFNNVMAYCTDVKSFPEESWKFLYKKKVLIIGCLKYDASFGHAHVDLCLDWVKELRPEVAILTHMSHDLEYYSLIDYIKSHSKDNIIVGYDGMQFNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSW3</accession>
    <name>Q3YSW3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304270.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0141"/>
    <dbReference type="EnsemblBacteria" id="AAZ68192">
        <property type="protein sequence ID" value="AAZ68192"/>
        <property type="gene ID" value="Ecaj_0141"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0141"/>
    <dbReference type="HOGENOM" id="CLU_114466_0_0_5"/>
    <dbReference type="OMA" id="FDWYTIL"/>
    <dbReference type="OrthoDB" id="1326940at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="210" mass="24377" checksum="768CB6DFD74D7D15" modified="2005-09-27" version="1">MCNNMLKRYCIIKISICVLLLITLSITLHYLYKSNAYILWHNLHIADEIQKLNFEIMDVHKHEAMLNDSGILWQEISTSNIYSTNTFYEENLGKLITNLCKKYYLFNSQINISSPKIVNHLYNKQYIDVIKSRVDINFSSISDEYVFLFLNAVRHDVSGYVKVVNFNIEKKTDITNEVLHSALKGETVATISGRISFDLYSIVGRFIDES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSX0</accession>
    <name>RL20_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplT</name>
        <name type="ordered locus">Ecaj_0134</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304263.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSX0"/>
    <dbReference type="SMR" id="Q3YSX0"/>
    <dbReference type="STRING" id="269484.Ecaj_0134"/>
    <dbReference type="EnsemblBacteria" id="AAZ68185">
        <property type="protein sequence ID" value="AAZ68185"/>
        <property type="gene ID" value="Ecaj_0134"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0134"/>
    <dbReference type="eggNOG" id="COG0292">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123265_0_1_5"/>
    <dbReference type="OMA" id="GRRKNVW"/>
    <dbReference type="OrthoDB" id="1932144at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07026">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00382">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005813">
        <property type="entry name" value="Ribosomal_L20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035566">
        <property type="entry name" value="Ribosomal_protein_L20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10986">
        <property type="entry name" value="PTHR10986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00453">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00062">
        <property type="entry name" value="RIBOSOMALL20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74731">
        <property type="entry name" value="SSF74731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01032">
        <property type="entry name" value="rplT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00937">
        <property type="entry name" value="RIBOSOMAL_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000243678" description="50S ribosomal protein L20">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00382"/>
        </source>
    </evidence>
    <sequence length="124" mass="14546" checksum="4D7430E0386E5D94" modified="2005-09-27" version="1">MARVKRGVTTRARHKKVIKLAKGYRGRSKNCYRIALQRVEKALQYAYRDRRNRKRFFRSLWIMRINAAARQHGLLYSDFIHGLSLANITLNRKVLADMAVNNQDNFKQIVDITKEALTKSRVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSX2</accession>
    <name>Q3YSX2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-oxoacyl-[acyl-carrier-protein] reductase FabG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">3-ketoacyl-acyl carrier protein reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4 6">Beta-Ketoacyl-acyl carrier protein reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Beta-ketoacyl-ACP reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0132</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304261.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0132"/>
    <dbReference type="EnsemblBacteria" id="AAZ68183">
        <property type="protein sequence ID" value="AAZ68183"/>
        <property type="gene ID" value="Ecaj_0132"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0132"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_11_0_5"/>
    <dbReference type="OMA" id="FWQDHFP"/>
    <dbReference type="OrthoDB" id="1821409at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="231" mass="26021" checksum="85AAA4483388DF33" modified="2005-09-27" version="1">MSQTVYKGALITGGARRLGKAIAMFLADNGYDIAIHYNTSESQALKTKDIIENLYNRKCILIQADLTEFNSLNLIIDETFQFMPYCNCLINNASVFYKNTLMNTSIQDFSYNYDLHIKAPLFLTQYFAKKCITIGNIINMIDAIVIKNATKSFTYTMSKKSLLDLTKFAAVELSPNIKVNAIGPRMIPGDFLDNINHKNIMDNAEVKYILTTIEKLLAPNNQETGTTIIMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSY4</accession>
    <name>Q3YSY4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Apolipoprotein N-acyltransferase</fullName>
            <shortName evidence="9">ALP N-acyltransferase</shortName>
            <ecNumber evidence="9">2.3.1.269</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">lnt</name>
        <name evidence="11" type="ordered locus">Ecaj_0120</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phospholipid dependent N-acylation of the N-terminal cysteine of apolipoprotein, the last step in lipoprotein maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a glycerophospholipid + N-terminal S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein] = a 2-acyl-sn-glycero-3-phospholipid + H(+) + N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48228"/>
            <dbReference type="Rhea" id="RHEA-COMP:14681"/>
            <dbReference type="Rhea" id="RHEA-COMP:14684"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136912"/>
            <dbReference type="ChEBI" id="CHEBI:140656"/>
            <dbReference type="ChEBI" id="CHEBI:140657"/>
            <dbReference type="ChEBI" id="CHEBI:140660"/>
            <dbReference type="EC" id="2.3.1.269"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; lipoprotein biosynthesis (N-acyl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CN hydrolase family. Apolipoprotein N-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.269" evidence="9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304249.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0120"/>
    <dbReference type="EnsemblBacteria" id="AAZ68171">
        <property type="protein sequence ID" value="AAZ68171"/>
        <property type="gene ID" value="Ecaj_0120"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0120"/>
    <dbReference type="eggNOG" id="COG0815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019563_3_1_5"/>
    <dbReference type="OMA" id="IPQSMKW"/>
    <dbReference type="OrthoDB" id="1291198at2"/>
    <dbReference type="UniPathway" id="UPA00666"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07571">
        <property type="entry name" value="ALP_N-acyl_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01148">
        <property type="entry name" value="Lnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004563">
        <property type="entry name" value="Apolipo_AcylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045378">
        <property type="entry name" value="LNT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20154">
        <property type="entry name" value="LNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00546">
        <property type="entry name" value="lnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="465"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="CN hydrolase" evidence="10">
        <location>
            <begin position="204"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="488" mass="55085" checksum="70C4DE7739087173" modified="2005-09-27" version="1">MTLGILGALSMAPFYMFIALMIAFSGLYIILSYSKNIKQAFFCGWCFGCGYFTTSLYWINVPLITQSKILWPLIPLTLIIPASLSLLFAAATATSYALNYKKLPGLITFSLSFTIVGMLLEYIVPWSLFAYSWAFSTEMLQTAALFGTYGMEFLAIFCSTAPGVCIKDKSTVSCAIVMLVLVSMFIYGNNRLLTNQDQKYNHDVAIRIVQGNTASNWDENEEQEYDKFQTYLRLTSQYGLNSRTHVIWGENSFPFLTHRNIDSVQSYLKLTIPEFLIAGGTRFANDKFYNTLFVINSNGQIIDHYDKFHLVPFGESIPSILVNLIPEKIASKSNYSPGTDKNRTISLNYSPFIPLICYESSFSHEVASRCTKGQWIVNITNDGWFGVSSQPYQHLEINRVRSIENGIPTIRAANSGISAVIDSYGRIITALPIMTEGIIDSYLPYHISEGTIYSKYLSKFITIKYATLIMLILVILRLILFKLHTNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSY9</accession>
    <name>Q3YSY9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Protein-export membrane protein SecG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0115</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in protein export. Participates in an early event of protein translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the SecG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304244.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0115"/>
    <dbReference type="EnsemblBacteria" id="AAZ68166">
        <property type="protein sequence ID" value="AAZ68166"/>
        <property type="gene ID" value="Ecaj_0115"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0115"/>
    <dbReference type="eggNOG" id="COG1314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094156_4_1_5"/>
    <dbReference type="OMA" id="FIHIVIS"/>
    <dbReference type="OrthoDB" id="1882472at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004692">
        <property type="entry name" value="SecG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34182">
        <property type="entry name" value="PTHR34182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03840">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01651">
        <property type="entry name" value="SECGEXPORT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00810">
        <property type="entry name" value="secG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0811">Translocation</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="110" mass="11837" checksum="3D3231B72A6583FB" modified="2005-09-27" version="1">MVLTVIQLVLVFLLVILILLQPPESDNLSGFGGTQGNLDSMFSTGSSSNFITKLTVAIATAFIINTVLLVGVNAKSVHKESIVKKIAEMSKAHNLPPMEHDTEDSVPFNQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT02</accession>
    <name>ISPF_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase</fullName>
            <shortName evidence="1">MECDP-synthase</shortName>
            <shortName evidence="1">MECPP-synthase</shortName>
            <shortName evidence="1">MECPS</shortName>
            <ecNumber evidence="1">4.6.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispF</name>
        <name type="ordered locus">Ecaj_0102</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP</text>
            <dbReference type="Rhea" id="RHEA:23864"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="4.6.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IspF family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304231.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT02"/>
    <dbReference type="SMR" id="Q3YT02"/>
    <dbReference type="STRING" id="269484.Ecaj_0102"/>
    <dbReference type="EnsemblBacteria" id="AAZ68153">
        <property type="protein sequence ID" value="AAZ68153"/>
        <property type="gene ID" value="Ecaj_0102"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0102"/>
    <dbReference type="eggNOG" id="COG0245">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084630_2_0_5"/>
    <dbReference type="OMA" id="QHFGTGR"/>
    <dbReference type="OrthoDB" id="1716560at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00095"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008685">
        <property type="term" value="F:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00554">
        <property type="entry name" value="MECDP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00107">
        <property type="entry name" value="IspF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003526">
        <property type="entry name" value="MECDP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020555">
        <property type="entry name" value="MECDP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036571">
        <property type="entry name" value="MECDP_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43181">
        <property type="entry name" value="PTHR43181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02542">
        <property type="entry name" value="YgbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69765">
        <property type="entry name" value="SSF69765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00151">
        <property type="entry name" value="ispF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01350">
        <property type="entry name" value="ISPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000237722" description="2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="17"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="49"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="76"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="147"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00107"/>
        </source>
    </evidence>
    <sequence length="173" mass="19160" checksum="89BD64E0B5F7C85F" modified="2005-09-27" version="1">MKQHINKPTFKVGIGYDVHKFDNIEYDATNTFITICGIKINHHKKIVAHSDGDVGLHALTDAILGAVGCGSIGQHFPNTDNKWKDAKSDYFLIEAKNKAQEKGYYISNADIIIICEQPKIMPHALKMQEYIANLIKIDTSCINVKATTTEKLGFLGRKEGIAAQAIVLCCLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT11</accession>
    <name>Q3YT11_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Competence protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304222.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0093"/>
    <dbReference type="EnsemblBacteria" id="AAZ68144">
        <property type="protein sequence ID" value="AAZ68144"/>
        <property type="gene ID" value="Ecaj_0093"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0093"/>
    <dbReference type="eggNOG" id="COG1040">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054549_0_0_5"/>
    <dbReference type="OMA" id="RYNQSAL"/>
    <dbReference type="OrthoDB" id="1937725at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044005">
        <property type="entry name" value="DZR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18912">
        <property type="entry name" value="DZR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DZR_2" evidence="2">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Pribosyltran" evidence="1">
        <location>
            <begin position="135"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="230" mass="26196" checksum="45C4ADDD67FA8A9D" modified="2005-09-27" version="1">MLGKIINIIFPRTCANCECTVPHYLDLCSICKNGIDFLHDNYCIGCGCRLPDGLSICGKCTVAPPKFTKLESVFSYNQYSKNMILNLKFFDNTLHIKTYGKWMYNKNPDLFNNVTTIIPVPIHKKRLRQRKYNQATLLAKALSKYCNIPLEIFVLKRIIDTVPQYSLSSQMREKNITQAFIVKNQHLITNKTILLVDDVITTGITARTCTNKLIEAGAKEVRVITLARTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT14</accession>
    <name>Q3YT14_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="5">ATase</shortName>
            <ecNumber evidence="5">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="5">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">purF</name>
        <name evidence="11" type="ordered locus">Ecaj_0090</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5 8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5 9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304219.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0090"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="EnsemblBacteria" id="AAZ68141">
        <property type="protein sequence ID" value="AAZ68141"/>
        <property type="gene ID" value="Ecaj_0090"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0090"/>
    <dbReference type="eggNOG" id="COG0034">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_1_5"/>
    <dbReference type="OMA" id="ENAQPTF"/>
    <dbReference type="OrthoDB" id="267682at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 6 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="10">
        <location>
            <begin position="10"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5 7">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="462" mass="51149" checksum="DD825EB8F75F810B" modified="2005-09-27" version="1">MQFKEVYEECGIFAIQNNRRAAVNCILGLHALQHRGQESFGIVTSEDNKLYCHYSNEQVNSMFNQQPKINLLLGKSAIGHVRYSTSGSKIGVQPITLDCKFGKLAIAHNGNLTNATQIRNSLTEKGCIFSSDIDTEVIAHLIAINTQATLLNNITNALKTINGAYSLVILVNGTIICCRDPAGIRPLVLGILDNSYIVASETCSLDIVGAQFVREISPGELIIIDQNNTLTSSFPFKKQKSSFCIFEYVYFARPDSIINNKSVYEIRKNIGKELAIENPVPKDTHMVVPVPDSGVPAALGFSEYTKIPFEFGIIRNHYVGRTFIQPSNYVRNMGVKLKHNANASILKDKIIILIDDSLVRGTTLKNIITLLHKAQVKEIHLRISSPPTINSCFYGIDTPEESKLIANKLSTTEIKNELGCDSIYFLSINGLYKAINNTTRNNSSPQYCDACFTGDYPIGKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT26</accession>
    <name>Q3YT26_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ecaj_0078</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC-4 integral membrane protein family. LolC/E subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304207.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0078"/>
    <dbReference type="EnsemblBacteria" id="AAZ68129">
        <property type="protein sequence ID" value="AAZ68129"/>
        <property type="gene ID" value="Ecaj_0078"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0078"/>
    <dbReference type="eggNOG" id="COG4591">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_1_5"/>
    <dbReference type="OMA" id="GWWQQNG"/>
    <dbReference type="OrthoDB" id="768211at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011925">
        <property type="entry name" value="LolCE_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02212">
        <property type="entry name" value="lolCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="311"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="10">
        <location>
            <begin position="27"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="9">
        <location>
            <begin position="297"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="410" mass="45495" checksum="96B099D657295FD4" modified="2005-09-27" version="1">MLLKFELMLALRYLQSSSSQFLCSMITILSLVGITLGVATLIIVMSVMNGFGEKLLDCVVGLNGHIAIYCDGNFDYDYHEITKSIKEIPGVKYAVPITENQAMIKSSSSVSGVIVRGIEKQDIERNNIIVNNIVRGDIAEFNDGIAIGIKLAEVLRVDYGDKLTIISSESLSTVIGNIPRMKTYKVVAIFDVGMYEFDSSLVYMPLHASQLFFHYDNRVKNIEVSIFDGNKSSEMLKLIENKIDMKGEDWKMQQGSYFHALEIERNVMFFILTLIIIVATFNIISSLSILVQDKKGAIAIMRTLGVTRCGILRIFCMCGVFIGLIGTVLGCVIGIVFSLNINAIKNILEKISNSNIFDPIIYFFDTLPSVLLVEDVVKISLLSLFLSLIAAILPARKAACQDPADILRHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT28</accession>
    <name>Q3YT28_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glutamate-cysteine ligase-related protein</fullName>
            <ecNumber evidence="1">6.3.2.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304205.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0076"/>
    <dbReference type="EnsemblBacteria" id="AAZ68127">
        <property type="protein sequence ID" value="AAZ68127"/>
        <property type="gene ID" value="Ecaj_0076"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0076"/>
    <dbReference type="eggNOG" id="COG0189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_635836_0_0_5"/>
    <dbReference type="OMA" id="GMHFVPL"/>
    <dbReference type="OrthoDB" id="481578at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011718">
        <property type="entry name" value="GshA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042520">
        <property type="entry name" value="GshA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08886">
        <property type="entry name" value="GshA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02049">
        <property type="entry name" value="gshA_ferroox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="408" mass="46347" checksum="606E5AC86612078A" modified="2005-09-27" version="1">MTIIIDTLNDILKTHELDIENWFSYRFTKYHPIINVSVDLRISDYKIAPVDTNIFPAGYNNFNQQSQVYTSRLLKEYIVNYMDCKKVLIIAENHTRNLKYVDSLITLKSIVNNAGFIVEVGICGINQNVELVSSNGDLINCLCLANNNGVLQAGCGFVPDLILLNNDMTSGIPEVLRGLKYQNIIPSLFLGWFNRSKSNHFSIYQELCKEFCSSFKIDPWLISTLFSSCSNICFIDGQGMHNIADEVEIIIDKIHNKFQLYGIKERPYVFIKADNGTYGMGIVVACCRDDVLTLNKKRRNKMKKIKDSEVVSKVIIQEGIATREMFNNCVAEPLIYFIGSTPSCYLYRYHNVKDKFSNLNSVGCNFIDVSYQKENMLSWSVIARIAALAAAIEMHSVANVNIIKQSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT39</accession>
    <name>Q3YT39_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304194.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68116">
        <property type="protein sequence ID" value="AAZ68116"/>
        <property type="gene ID" value="Ecaj_0065"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0065"/>
    <dbReference type="HOGENOM" id="CLU_458384_0_0_5"/>
    <dbReference type="OMA" id="IFRARIF"/>
    <dbReference type="OrthoDB" id="2109655at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021902">
        <property type="entry name" value="DUF3514"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12027">
        <property type="entry name" value="DUF3514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="595" mass="65435" checksum="7404A08C968B7E7F" modified="2005-09-27" version="1">MLSGNNNPSKKESQVQDENTQSSGGVQEDSEQSEGTKKDVQSSEKQDSASKDDVKASSDERKGGGGEETSKNVGASDLESTGGDAASGVQEQYLRQGARPKVRASRTVKAKKHKSPKTSVAPAVQFFDWDSGAYCEEDPFLVKEATKELSKDTKPTTGVVQPQATEGILPDDSEKEGEASKKLGIVEQSFDGAEEQLVKLVEEISIGELGAAAPLESDEQSGIGTIDPDTKIAEVSAVSVLVDPRKPDKKNIFYGRASLQAFRQNRLVAPSVEAYHIGVRQLKEKLNPFREMHLQYCCHMMQGIFCMVGNKVHVNKNVDDFLKSKVIDHAMLVFVLKLGCLMNCVAILGKNNFFSSLAYDAQLYWDKSVLNTLLKKLLASISAAQISIPVVNFHFSQLLNQGDQAAHSGTFYRRVLSVLCQLVRLRLCDPSCENQHNMLDLGIVSCAMIMGTMYKQYISMLHFSGDVNGHPSFSQVITCMGPQRPHRGVFARLCVTILCKLSGMNNLKYNKAVLFNSLYMPGGLIANASEHFLGKITQCINNNEMFFGVIIEDLSMQVCMMLDRMHLAAMEEEIEQYCVAYSKVTHGYSSQGPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT50</accession>
    <name>Q3YT50_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">L-glutamine synthetase</fullName>
            <ecNumber evidence="8">6.3.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0054</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Oligomer of 12 subunits arranged in the form of two hexameric ring.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0054"/>
    <dbReference type="EnsemblBacteria" id="AAZ68105">
        <property type="protein sequence ID" value="AAZ68105"/>
        <property type="gene ID" value="Ecaj_0054"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0054"/>
    <dbReference type="eggNOG" id="COG0174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_2_5"/>
    <dbReference type="OMA" id="PHPHEFE"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_b-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001637">
        <property type="entry name" value="Gln_synth_I_adenylation_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="SSF54368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00182">
        <property type="entry name" value="GLNA_ADENYLATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="7">
        <location>
            <begin position="124"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-AMP-tyrosine" evidence="5">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="490" mass="55734" checksum="6B88756F5C9CD950" modified="2005-09-27" version="1">MLQKHKKLDILSVFKLGNLIMFLSTEDTLNYIKKNAINFIDLRFTDSNGRWHHITRSAETTDAQVLLTGINFDSSSISGWQSIEKSDMILLPDISTAFVDPFSAQSTLVIICNIVDPYNQSQYAKDPRYTAQKAYEYMLSTGIADKCYFGPEVEFFVFDNVQFYTGMYDSYFKIESAEHDISYQRREFSNNNHRPRVKGGYMPTPPIDSLHDIRSEMLTMFKEVGITPLIHHHEVAASQCEVGFQYDELLASADNVQKCKYIVHGVTSSYGKTATFMPKPVIKDNGSGMHCHQSLWKDNVNLFVNSDGNISDTCLYYIGGIIKYGKAINAFTNPSTNSYKRLVPHFEAPTWLTYSYENRSAAIRIPYVPQNDVNAKRIEVRFPDPLANPYLCFSAQLMAGLYGIQNKIHPGPAIDKNLYSLNKAELQQLSSVANSLEDSLESLDKDREFLLAGNVFTNDQIDSYINLKTLEVKDLQLHPHPIEFINYYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT57</accession>
    <name>Q3YT57_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304176.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68098">
        <property type="protein sequence ID" value="AAZ68098"/>
        <property type="gene ID" value="Ecaj_0047"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0047"/>
    <dbReference type="HOGENOM" id="CLU_157126_0_0_5"/>
    <dbReference type="OMA" id="LWYSLKG"/>
    <dbReference type="OrthoDB" id="2093414at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="133" mass="14397" checksum="9C10D88D474BDE42" modified="2005-09-27" version="1">MTTTLSKRKTPTTKSTSKTKNKKNTSTTATTKSIRSKTSFEDKIQATTSTPPSNSSNISDSSSISSISTNNSSSSNSSTSSTDSTLNNMNSINLKKVESDESEIRYITPGSSKESSFLKDLWYSLKGYFSSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT73</accession>
    <name>Q3YT73_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.117</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Tetrahydrodipicolinate N-succinyltransferase</fullName>
            <shortName evidence="7">THDP succinyltransferase</shortName>
            <shortName evidence="7">THP succinyltransferase</shortName>
            <shortName evidence="7">Tetrahydropicolinate succinylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dapD</name>
        <name evidence="9" type="ordered locus">Ecaj_0031</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + succinyl-CoA = (S)-2-succinylamino-6-oxoheptanedioate + CoA</text>
            <dbReference type="Rhea" id="RHEA:17325"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15685"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="2.3.1.117"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.117" evidence="7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0031"/>
    <dbReference type="EnsemblBacteria" id="AAZ68082">
        <property type="protein sequence ID" value="AAZ68082"/>
        <property type="gene ID" value="Ecaj_0031"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0031"/>
    <dbReference type="eggNOG" id="COG2171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050859_0_1_5"/>
    <dbReference type="OMA" id="YFPIQKM"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00019"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008666">
        <property type="term" value="F:2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.166.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00811">
        <property type="entry name" value="DapD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005664">
        <property type="entry name" value="DapD_Trfase_Hexpep_rpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023180">
        <property type="entry name" value="THP_succinylTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037133">
        <property type="entry name" value="THP_succinylTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14805">
        <property type="entry name" value="THDPS_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00965">
        <property type="entry name" value="dapD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THDPS_N_2" evidence="8">
        <location>
            <begin position="9"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="281" mass="30561" checksum="600958B074934967" modified="2005-09-27" version="1">MSMIDVCDFKEVIEDAWSNLANLSSNTSIKKVVDDVMDLLDQGRIRVCEKVGDQWVVNEWAKKAILLCFRVYDMNLAEINTSNSMLGNLCWFDKVQLKFGKWSADDFRRAKIRAVPGSMVRKSAYIAPNVVLMPSFVNVGAYVGEGTMIDTWASVGSCAQIGKHCHISGGAGIGGVLEPLSSRPVVIEDNCFIGARSEIVEGVIVGEGSVISMGVYIGASTRIVDRESGEISYGRIPPYSVVVPGSYGSGNLSLYCAVIVKKVDDNTRSKVSINELLRDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT76</accession>
    <name>Q3YT76_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 10">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="2 7 10">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pgk</name>
        <name evidence="11" type="ordered locus">Ecaj_0028</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7 10">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="2 7 10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0028"/>
    <dbReference type="EnsemblBacteria" id="AAZ68079">
        <property type="protein sequence ID" value="AAZ68079"/>
        <property type="gene ID" value="Ecaj_0028"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0028"/>
    <dbReference type="eggNOG" id="COG0126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_5"/>
    <dbReference type="OMA" id="DMIFDIG"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7 8">
        <location>
            <begin position="30"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7 8">
        <location>
            <begin position="68"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="360"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="394" mass="43659" checksum="00142133F763F472" modified="2005-09-27" version="1">MLRSHDFTFCNMKKIQDFSCSGKTVLLRADLNVPMDNGVVLDDTRIVRLTTTIKYLLSNDAKIVIMSHYGRPKSYDKEFSLKFLIEYLNKIFTTNVIFIDGVIGDYVEQTIQSASLGSILLLENLRFYAEEETNDLAFAKQLALLADIYVNDAFSCLHRKHASIDAITRLIPSFIGFNFQEEMKYLNCVVSNSEKPVAVIVGGSKISTKIHMLKNLTKKVDFLIVGGAIANNFLLSQGLKIGRSLYEELEKDLVKEIMDLAKRHECKIIVPVDYVVAKNSIYGKSSIKDNDTIESDDVILDIGPQTINIVTATVNKCRTILWNGPCGMFEKEQFSKGTFSVANLLAKLTKAGKLKSIVGGGDSICAIKLSGLSNDDFTYISTGGGALLHFLSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT90</accession>
    <name>Q3YT90_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Methylase involved in ubiquinone/menaquinone biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304143.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0014"/>
    <dbReference type="EnsemblBacteria" id="AAZ68065">
        <property type="protein sequence ID" value="AAZ68065"/>
        <property type="gene ID" value="Ecaj_0014"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0014"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046586_2_2_5"/>
    <dbReference type="OMA" id="SWNIMYL"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="52"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="253" mass="29027" checksum="B6A805AA17C57616" modified="2005-09-27" version="1">MIVQYESYTQKIQKAFSGAADSYDKFSYIQDVVLRELCSAVQLKDCDKKNILDVGCGTGNISKFLDITNHNFIQVDLSKEMCVVAKEKNNVLSVNCNMDMMPFCENLFDIVIASMVLQWSCNINLSLLELLRVIKPNGMLYIAIPIFGTLIELNNVIEKIGKSFSKFYQMDELISVINSLDVKIQCVFCCNYRQYHKSFLSLLLSMKSTGAYAKKVYDKQYNIFSVSSIYKKLYSLQNCVFSSWNIMYLIVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQM9</accession>
    <name>Q3YQM9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methionine adenosyltransferase</fullName>
            <ecNumber evidence="6">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Ecaj_0945</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305049.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0945"/>
    <dbReference type="EnsemblBacteria" id="AAZ68976">
        <property type="protein sequence ID" value="AAZ68976"/>
        <property type="gene ID" value="Ecaj_0945"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0945"/>
    <dbReference type="eggNOG" id="COG0192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041802_1_1_5"/>
    <dbReference type="OMA" id="MPYLRPD"/>
    <dbReference type="OrthoDB" id="1024388at2"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="PTHR11964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="SSF55973"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="13 14" id="KW-0630">Potassium</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-AdoMet_synt_N" evidence="16">
        <location>
            <begin position="10"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="S-AdoMet_synt_M" evidence="17">
        <location>
            <begin position="119"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="S-AdoMet_synt_C" evidence="18">
        <location>
            <begin position="237"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AAZ68976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="393" mass="43446" checksum="FD9EC23BC184D756" modified="2005-09-27" version="1">MFYYQNCSSKYIITSESVSSGHPDKIADQISDAILDYYISLNPIVHAAIETLVTKNKVIISGEICGVNVNNSDIECITRQVIKDIGYERDGFHWNYVVIEILIHEQSQDIVAGVNFGKNQGAGDQGIVYGYAINETETFMPASIFYSHLILKNIISAVKNFEIPNLGPDAKTQITLLYENNVPVKASNIVLSIQHSEDISQSQIRDIVYPYVKNTLPDGWMCSDSDFLVNPAGRFVIGGPVGDCGLTGRKIMIDTYGGHIPHGGGAFSGKDPSKVDRSAAYMARYLAKNVVSAKLANQCLVQLSYAIGISHPISFYVNTFGTGVVSDDIIKSFIENNIDLSPYGICKHLMLLNPIYKITSCYGHFGRIPGENGSFSWEMEDFALKLRNEFYLN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQP2</accession>
    <name>RL19_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplS</name>
        <name type="ordered locus">Ecaj_0932</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305036.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YQP2"/>
    <dbReference type="SMR" id="Q3YQP2"/>
    <dbReference type="STRING" id="269484.Ecaj_0932"/>
    <dbReference type="EnsemblBacteria" id="AAZ68963">
        <property type="protein sequence ID" value="AAZ68963"/>
        <property type="gene ID" value="Ecaj_0932"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0932"/>
    <dbReference type="eggNOG" id="COG0335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103507_2_1_5"/>
    <dbReference type="OMA" id="SPMIESV"/>
    <dbReference type="OrthoDB" id="1698718at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00402">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="L19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_L19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="PTHR15680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002191">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00061">
        <property type="entry name" value="RIBOSOMALL19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01024">
        <property type="entry name" value="rplS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000226846" description="50S ribosomal protein L19">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00402"/>
        </source>
    </evidence>
    <sequence length="125" mass="14438" checksum="5728A80C35D32C35" modified="2005-09-27" version="1">MSNLLKEFNEQQMKLLSNKEIPKFSAGDTLRVSMKIFDGVSERIQVFEGVCIKRRNNGLHSSFTLRKISYNESIQLQVFLYSPTVESIEVVKFGKVRRAKLYYMLSLFGKSARIKERSNVARHAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-04-17" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQP7</accession>
    <name>YBEY_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoribonuclease YbeY</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ybeY</name>
        <name type="ordered locus">Ecaj_0927</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the endoribonuclease YbeY family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305031.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YQP7"/>
    <dbReference type="SMR" id="Q3YQP7"/>
    <dbReference type="STRING" id="269484.Ecaj_0927"/>
    <dbReference type="EnsemblBacteria" id="AAZ68958">
        <property type="protein sequence ID" value="AAZ68958"/>
        <property type="gene ID" value="Ecaj_0927"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0927"/>
    <dbReference type="eggNOG" id="COG0319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106710_0_0_5"/>
    <dbReference type="OMA" id="RMRIHPL"/>
    <dbReference type="OrthoDB" id="1830156at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00009">
        <property type="entry name" value="Endoribonucl_YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023091">
        <property type="entry name" value="MetalPrtase_cat_dom_sf_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002036">
        <property type="entry name" value="YbeY"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02130">
        <property type="entry name" value="YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00043">
        <property type="entry name" value="TIGR00043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000284201" description="Endoribonuclease YbeY">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00009"/>
        </source>
    </evidence>
    <sequence length="154" mass="18312" checksum="64D6155789922960" modified="2005-09-27" version="1">MIEINVYYKKWYNVIRKPKSFVKNIINTSLIDLNIYEYKPIISIVLANNKLLQQLNYEYRKKNKPTNVLSFPYNKLDKNCYLGEIFISLDVLMNESVDLNIPIEHHTSHMLIHGLLHILDYDHEEPLDQYIMESIEIKLLDKLGIKNPYVSRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQQ9</accession>
    <name>Q3YQQ9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305019.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0915"/>
    <dbReference type="EnsemblBacteria" id="AAZ68946">
        <property type="protein sequence ID" value="AAZ68946"/>
        <property type="gene ID" value="Ecaj_0915"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0915"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="FRDIPAM"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230954" evidence="1">
        <location>
            <begin position="20"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="280" mass="30776" checksum="BE2852F037E68A80" modified="2005-09-27" version="1">MNCKKILITTALMSLMYYAPSISFSDTIQDDNTGSFYISGKYVPSVSHFGVFSAKEERNSTVGVFGLKHDWNGGTISNSSPENIFTVQNYSFKYENNPFLGFAGAIGYSMGGPRIELEVLYETFDVKNQNNNYKNGAHRYCALSHHSSATSMSSASNKFVFLKNEGLIDLSFMINACYDIIIEGMPFSPYICAGVGTDVVSMFEAINPKISYQGKLGLGYSISSEASVFIGGHFHRVIGNEFRDIPAMVPSGSNLPENQFAIVTLNVCHFGLELGGRFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQR9</accession>
    <name>Q3YQR9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305012.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0905"/>
    <dbReference type="EnsemblBacteria" id="AAZ68936">
        <property type="protein sequence ID" value="AAZ68936"/>
        <property type="gene ID" value="Ecaj_0905"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0905"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="LAYYTIM"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230956" evidence="1">
        <location>
            <begin position="26"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="279" mass="31379" checksum="07E356825E071E80" modified="2005-09-27" version="1">MNKKKIITVGTTLAYLLLSPNISFSEVINNDTDKYSRLYISGQYKPGFSYFNKFSVRETDHFTKALIGLRHDAISTKNLTTNTDFNTLYKVTFQNNIISFSGAIGYSDSTGVRFELEGSYEEFDVTDPGDCIIKDTYRYFALARKTSGNHPNDNGEYTVMRNDGVSITSVIFNGCYDLSLKELEISPYVCIGIGGDFIEFFDALHIKLAYQGKLGISYSFSTRTNLFIDCYYHRVIGNQFNNLNVQHVVELTEAPKATSAIATLNVSYFGGEVGIRLMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQU5</accession>
    <name>Q3YQU5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent zinc metalloprotease FtsH</fullName>
            <ecNumber evidence="7">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ftsH</name>
        <name evidence="10" type="ordered locus">Ecaj_0879</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the central section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304986.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YQU5"/>
    <dbReference type="STRING" id="269484.Ecaj_0879"/>
    <dbReference type="EnsemblBacteria" id="AAZ68910">
        <property type="protein sequence ID" value="AAZ68910"/>
        <property type="gene ID" value="Ecaj_0879"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0879"/>
    <dbReference type="eggNOG" id="COG0465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_16_2_5"/>
    <dbReference type="OMA" id="QYGMTER"/>
    <dbReference type="OrthoDB" id="190468at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 7 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="9">
        <location>
            <begin position="188"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="196"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="611" mass="67733" checksum="20024F5A044BD371" modified="2005-09-27" version="1">MRKIIEGLAIWIIVIVLVAAAYVQFNDKVINGNVLKLPFSEFLNKIDNNEIETINISEHNITGKLKDSAKFQTTGVIYDSLIKTLHSKQVTFTFLPEDTLFGILGSILISWFPMLLLVIIWFIFLKRMQIGGNRTINFSKSRAKLMTEHRNKVTFNDVAGIDEAKEELIEIVDFLKHRQRFQKLGGKIPKGCLLIGSPGTGKTLLARAIAGEANVPFFSISGSDFVEMFVGVGASRVRDMFEQGKKNAPCIIFIDEIDAVGRHRGIGLGGGNDEREQTLNQLLVEMDGFESNEGVIIIAATNRPDVLDSALLRPGRFDRQVTISIPDINGREKIINVHIKKVPTAPDVDIRTIARGTPGFSGADLANLVNEAALIAARLNKKIVTMSDFEYARDKVMMGAERKSLMMTEEEKKLTAYHEAGHAVIAFFTVASDPIHKATIIPRGRSLGLVMRLPESDRVSHTREKMTADLTVAMGGRAAEELIFGYHKVTSGASSDIKQATDLARAMVMKWGMSDKVGPLYHSDEKNETISNNLANLIDEEVKSIVTSALERAKSLLHEHLESLHIVAKNLLEFETLTGEDIKNVINGKELTKNNEDEAQPLKRSFASKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQU9</accession>
    <name>Q3YQU9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304982.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0875"/>
    <dbReference type="EnsemblBacteria" id="AAZ68906">
        <property type="protein sequence ID" value="AAZ68906"/>
        <property type="gene ID" value="Ecaj_0875"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0875"/>
    <dbReference type="HOGENOM" id="CLU_138419_0_0_5"/>
    <dbReference type="OMA" id="INLAFCI"/>
    <dbReference type="OrthoDB" id="1595638at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="161" mass="18493" checksum="39BAC524E0968397" modified="2005-09-27" version="1">MISSYFIINLAFCIGALLVFRPLYRKINQFLSTRLNKVKQEVTLPSEVQKQSKDLLDSVVVQNLETEKILKEILKKAHEEYDLIITSNKKDIENILEKHLDVAIKKISHQVSAMTQSLKLSTIDVATSAIQELIKESNYNQKQDNNGVISTLDRDLRKKLH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-02-10" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQV2</accession>
    <name>ATP6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F0 sector subunit a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpB</name>
        <name type="ordered locus">Ecaj_0872</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase A chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304980.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YQV2"/>
    <dbReference type="SMR" id="Q3YQV2"/>
    <dbReference type="STRING" id="269484.Ecaj_0872"/>
    <dbReference type="EnsemblBacteria" id="AAZ68903">
        <property type="protein sequence ID" value="AAZ68903"/>
        <property type="gene ID" value="Ecaj_0872"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0872"/>
    <dbReference type="eggNOG" id="COG0356">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041018_0_2_5"/>
    <dbReference type="OMA" id="FFDQFMS"/>
    <dbReference type="OrthoDB" id="867266at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01393">
        <property type="entry name" value="ATP_synth_a_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023011">
        <property type="entry name" value="ATP_synth_F0_asu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045083">
        <property type="entry name" value="ATP_synth_F0_asu_bact/mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410">
        <property type="entry name" value="PTHR11410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00123">
        <property type="entry name" value="ATPASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="SSF81336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01131">
        <property type="entry name" value="ATP_synt_6_or_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00449">
        <property type="entry name" value="ATPASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0138">CF(0)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000362289" description="ATP synthase subunit a">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01393"/>
        </source>
    </evidence>
    <sequence length="243" mass="26886" checksum="AF30B400A258A628" modified="2005-09-27" version="1">MSASPLDQFKILTIFKLPNIAGYNIDFTNASLFMVLSTISVALFCYIGLKKESIIPNGIQSIVEFIYEFIVSTIESNVGKEGLQYIPLVFTIFMFIATCNLLGILPLGFTATSHIAVTFAISMVVFVSVTIIGFKHQGIHFLRILLPQGTPGWLAPMMVFIELFAYCARPVSLSIRLAANMIAGHTIIKVIAGFVVKMNIFLTPLPMIFIIILIGFEIFVAILQAYIFTVLTCVYLSDAVKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQX1</accession>
    <name>Q3YQX1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Riboflavin biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="12">Riboflavin kinase</fullName>
                <ecNumber evidence="12">2.7.1.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="12">Flavokinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="12">FMN adenylyltransferase</fullName>
                <ecNumber evidence="12">2.7.7.2</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="12">FAD pyrophosphorylase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="12">FAD synthase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ecaj_0853</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the phosphorylation of riboflavin to FMN followed by the adenylation of FMN to FAD.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 12">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + riboflavin = ADP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 12">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ribF family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="12"/>
    <dbReference type="EC" id="2.7.7.2" evidence="12"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304961.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0853"/>
    <dbReference type="EnsemblBacteria" id="AAZ68884">
        <property type="protein sequence ID" value="AAZ68884"/>
        <property type="gene ID" value="Ecaj_0853"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0853"/>
    <dbReference type="eggNOG" id="COG0196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048437_0_2_5"/>
    <dbReference type="OMA" id="HRGHQAI"/>
    <dbReference type="OrthoDB" id="1504821at2"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02064">
        <property type="entry name" value="FAD_synthetase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015864">
        <property type="entry name" value="FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002606">
        <property type="entry name" value="Riboflavin_kinase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="PTHR22749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06574">
        <property type="entry name" value="FAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004491">
        <property type="entry name" value="FAD_Synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00083">
        <property type="entry name" value="ribF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0274">FAD</keyword>
    <keyword evidence="6 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 12" id="KW-0288">FMN</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavokinase" evidence="13">
        <location>
            <begin position="182"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR004491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAZ68884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="305" mass="34665" checksum="9A16597ADE35B2A1" modified="2005-09-27" version="1">MKIIYGYPSEKSDLSSVLAFGNFDGVHLGHKSIINTVKNISIKEDVASAIITFAPHPAEYLHGRKNFLLLNFEQKIELLQSYEIDYLYIIDFNQSFSQLSPDDFIKDVLVSSCNVKYIVTGHNCFFGYKCLGNVDLLYAYADIYNYEVVQINPVFINDDILCSSSLIRECLSEGKLDLANKILGRPYQISGKVIRGLARGRIIGFPTINVAIEHMLPKVGVYSACVKINNTTTWLNGIVNIGLRPTFGDLNVPILEMHIFDFSDDIYDQYVTIQLLDFIRPERKFDTVDQLKRQINNDIVQVKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQX2</accession>
    <name>Q3YQX2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein signal peptidase</fullName>
            <ecNumber evidence="6">3.4.23.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prolipoprotein signal peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Signal peptidase II</fullName>
            <shortName evidence="6">SPase II</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lspA</name>
        <name evidence="9" type="ordered locus">Ecaj_0852</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">This protein specifically catalyzes the removal of signal peptides from prolipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.</text>
            <dbReference type="EC" id="3.4.23.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; lipoprotein biosynthesis (signal peptide cleavage).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the peptidase A8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.36" evidence="6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304960.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0852"/>
    <dbReference type="EnsemblBacteria" id="AAZ68883">
        <property type="protein sequence ID" value="AAZ68883"/>
        <property type="gene ID" value="Ecaj_0852"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0852"/>
    <dbReference type="eggNOG" id="COG0597">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083252_4_3_5"/>
    <dbReference type="OMA" id="CICYLIT"/>
    <dbReference type="OrthoDB" id="1575081at2"/>
    <dbReference type="UniPathway" id="UPA00665"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00161">
        <property type="entry name" value="LspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001872">
        <property type="entry name" value="Peptidase_A8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33695">
        <property type="entry name" value="PTHR33695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01252">
        <property type="entry name" value="Peptidase_A8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00781">
        <property type="entry name" value="LIPOSIGPTASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00077">
        <property type="entry name" value="lspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00855">
        <property type="entry name" value="SPASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="153" mass="17583" checksum="210992D1144C667F" modified="2005-09-27" version="1">MKKYVLIICLIIFIDQVSKWYVVNLIGDKGVIEILSFLRFTTVWNAGISFGILNNFEYSNVVFCSISILITCVLCYLFIVQPHYRLPLVIIIGGSIGNIIDRIRYGAVYDFIDFYINNLHWPVFNLADSFIFLGIVIIMAKSNNHMKQINCNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQX4</accession>
    <name>Q3YQX4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Insulinase-like:Peptidase M16, C-terminal</fullName>
            <ecNumber evidence="5">3.4.24.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0850</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304958.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0850"/>
    <dbReference type="EnsemblBacteria" id="AAZ68881">
        <property type="protein sequence ID" value="AAZ68881"/>
        <property type="gene ID" value="Ecaj_0850"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0850"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_6_2_5"/>
    <dbReference type="OMA" id="RLMRYEY"/>
    <dbReference type="OrthoDB" id="1077305at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230961" evidence="2">
        <location>
            <begin position="21"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16" evidence="3">
        <location>
            <begin position="57"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="4">
        <location>
            <begin position="191"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="451" mass="50943" checksum="86E21C52A3F0692F" modified="2005-09-27" version="1">MRNILCYTLILIFFSFNTYANDLNINIKEATTKNKIHYLYVEHHNLPTISLKFAFKKAGYAYDAFDKQGLAYFTSKILNEGSKNNYALSFAQQLEGKGIDLKFDIDLDNFYISLKTLSENFEEALVLLSDCIFNTVTDQEIFNRIIAEQIAHVKSLYSAPEFIATTEMNHAIFKGHPYSNKVYGTLNTINNINQEDVALYIKNSFDKEQIVISAAGDVDPTQLSNLLDKYILSKLPSGNNKNTIPDTTVNREDTLLYVQRDVPQSVIMFATDTVPYHSKDYHASNLFNTMLGGLSLNSILMIELRDKLGLTYHSSSSLSNMNHSNVLFGTIFTDNTTVTKCISVLTDIIEHIKKYGVDEDTFAIAKSSITNSFILSMLNNNNVSEILLSLQLHDLDPSYINKYNSYYKAITIEEVNKIAKKILSNELVIIEVGKNNNINGKQIDAKKHILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQX5</accession>
    <name>Q3YQX5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Insulinase-like:Peptidase M16, C-terminal</fullName>
            <ecNumber evidence="5">3.4.24.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0849</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304957.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0849"/>
    <dbReference type="EnsemblBacteria" id="AAZ68880">
        <property type="protein sequence ID" value="AAZ68880"/>
        <property type="gene ID" value="Ecaj_0849"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0849"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_1_0_5"/>
    <dbReference type="OMA" id="FNGSTHL"/>
    <dbReference type="OrthoDB" id="1188251at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230962" evidence="2">
        <location>
            <begin position="20"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16" evidence="3">
        <location>
            <begin position="42"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="4">
        <location>
            <begin position="189"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="438" mass="49818" checksum="F0235DA486905747" modified="2005-09-27" version="1">MMKFFTCFFIVFLTIANHALSFNIKVTHEKLDNGMEVYVIPNHRAPAVMHMVLYKVGGTDDPVGYSGLAHFFEHLMFSGTEKFPNLISTLSNIGGNFNASTSQFCTIYYELIPKQYLSLAMDIESDRMQNFKVTDKALIREQKVVLEERKMRVESQAKNILEEEMENAFYYNGYGRPVVGWEHEISNYNKEVAEAFHKLHYSPNNAILIVTGDADPQEVITLAKQYYGKIPSNNKKPSSQVRVEPPHKTNMTLTLKDSSVEIPELFLMYQIPNGITNKNYILNMMLAEILGSGKFSLLYNDLVINNPIVTSIKTDYNYLTDSDNYLSIEAIPKNGISTEAVEQEIHKCINNYLENGISAEYLESAKYKVKAHLTYAFDGLTFISYFYGMHLILGVPLSEISNIYDTIDKVSIQDVNSAMENIFQNNIRLTGHLLPNGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQX7</accession>
    <name>RNC_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease 3</fullName>
            <ecNumber evidence="1">3.1.26.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribonuclease III</fullName>
            <shortName evidence="1">RNase III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnc</name>
        <name type="ordered locus">Ecaj_0847</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonuclease III family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304955.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YQX7"/>
    <dbReference type="SMR" id="Q3YQX7"/>
    <dbReference type="STRING" id="269484.Ecaj_0847"/>
    <dbReference type="EnsemblBacteria" id="AAZ68878">
        <property type="protein sequence ID" value="AAZ68878"/>
        <property type="gene ID" value="Ecaj_0847"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0847"/>
    <dbReference type="eggNOG" id="COG0571">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000907_1_1_5"/>
    <dbReference type="OMA" id="LTHKSCK"/>
    <dbReference type="OrthoDB" id="1890943at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00104">
        <property type="entry name" value="RNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011907">
        <property type="entry name" value="RNase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="PTHR11207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02191">
        <property type="entry name" value="RNaseIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0507">mRNA processing</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000228527" description="Ribonuclease 3">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="2"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="1">
        <location>
            <begin position="154"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00104"/>
        </source>
    </evidence>
    <sequence length="226" mass="25271" checksum="3916217415DC3E8C" modified="2005-09-27" version="1">MIETISKTIKYHFKNPQLLHEALTHPSIISKDSLKFNYERLEFLGDAVLNIVISEMLFNIFPKDTEGNLAKKKTALVCGNQLVKVAQSINLGQFIIMSDGERTCGGANNHNNLENALEALIGAIYLDGGLTAAQNFIHSFWKHTAIHMDIPPQDAKTILQEFIQGKRLPAPTYHIIDKSGPDHNPIFTVELRIPLYKTIQATGNNKKLAEQKAASLMLNQIKDQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR21</accession>
    <name>Q3YR21_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription termination factor Rho</fullName>
            <ecNumber evidence="8">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP-dependent helicase Rho</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rho</name>
        <name evidence="12" type="ordered locus">Ecaj_0803</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. The homohexamer assembles into an open ring structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304911.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0803"/>
    <dbReference type="EnsemblBacteria" id="AAZ68834">
        <property type="protein sequence ID" value="AAZ68834"/>
        <property type="gene ID" value="Ecaj_0803"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0803"/>
    <dbReference type="eggNOG" id="COG1158">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016377_4_3_5"/>
    <dbReference type="OMA" id="NTIAYNH"/>
    <dbReference type="OrthoDB" id="1619125at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008186">
        <property type="term" value="F:ATP-dependent activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04459">
        <property type="entry name" value="Rho_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01128">
        <property type="entry name" value="rho_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01884">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041703">
        <property type="entry name" value="Rho_factor_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011112">
        <property type="entry name" value="Rho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036269">
        <property type="entry name" value="Rho_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011113">
        <property type="entry name" value="Rho_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004665">
        <property type="entry name" value="Term_rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46425">
        <property type="entry name" value="PTHR46425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07498">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07497">
        <property type="entry name" value="Rho_RNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00959">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68912">
        <property type="entry name" value="SSF68912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00767">
        <property type="entry name" value="rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51856">
        <property type="entry name" value="RHO_RNA_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 8" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="Rho RNA-BD" evidence="11">
        <location>
            <begin position="96"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="220"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="232"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="469" mass="52126" checksum="03D8FBB68CB567EE" modified="2005-09-27" version="1">MSESEDVVLDREVLTPEVLEKQEDKSEEVGDKEEVEGSAQVEAQEVKVLDLSELKKKSIEDLLKIAEELGVVSNGRMLKQEIIFHLMKKVVNDGGAAIGGGVVEILSDGFGFLRSPEANYAASGDDVYISAGQIKKFNLRTGDIVSGEIRAPSEKERYFTLVKAHSINFTDMGKLQRYVHFDDLIPLYPEERILLETNDPVSLSKKDISMRVIDIVAPLGKGQRALIVAPPRAGKTIILQQIAHSISVNHPDIELIVLLIGERPEEVTDMCRSVKGEVVSSTFDEPGYRHVQLAEIVIEKAKRMVEHKKNVVILLDSITRLARAYNSVIPSSGKVLTGGVDSNALQRPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNCEIILDRKISDKRVYPAIDISKSGTRKEDMLIDHVCLKKVWLLRRLLSSMGSVEAMEFLRDKLSITKDNNEFFDMMNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR45</accession>
    <name>Q3YR45_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0779</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304887.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0779"/>
    <dbReference type="EnsemblBacteria" id="AAZ68810">
        <property type="protein sequence ID" value="AAZ68810"/>
        <property type="gene ID" value="Ecaj_0779"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0779"/>
    <dbReference type="eggNOG" id="COG2271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_62_2_5"/>
    <dbReference type="OMA" id="VYAGMQI"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="8"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="411" mass="45154" checksum="0CA4AED77415FE38" modified="2005-09-27" version="1">MGDVLNRKFLAWFLVSMFYAYQYILRVLPNVIASISIEKFKISAIAFGQFSGLYYIGYTLAHIPIGIFLDKYGPRVVLPICVVLTFIGLVPLLISDVWLFAQIGRIVTGIGSAGSALGLFKVANMYYNNRFARMSGISIIIGLLGAMYGGLPVLSLLDKLGWENLFLIFIAIGAVLAVLLYVFMLPYDRGGIDDASNSILSNIKLVICNKYIIIISLLAGFMIGPLEGFADGWVTSFLRAVGNMDKEASALLPSTIFIGVCFGAFILPYMFENMSFNGWNLLIASGFGMMIPFLILYINSSNALLVTVLFFLIGFFSAYQIITTCKVLSYVNDSVVALATAVNNMIAMAFGYFFHTAISCVIDVLWDGKIVGSEPVYTKALMLKSMLFVPGGLLIGIIGFVYLKYTVRENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR48</accession>
    <name>Q3YR48_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin-like_fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0776</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304884.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0776"/>
    <dbReference type="EnsemblBacteria" id="AAZ68807">
        <property type="protein sequence ID" value="AAZ68807"/>
        <property type="gene ID" value="Ecaj_0776"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0776"/>
    <dbReference type="eggNOG" id="COG1651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_47_5_5"/>
    <dbReference type="OMA" id="EYASFSC"/>
    <dbReference type="OrthoDB" id="1678187at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230971" description="Thioredoxin-like_fold domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin-like_fold" evidence="2">
        <location>
            <begin position="57"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="239" mass="27269" checksum="828AAE83A7C2EE68" modified="2005-09-27" version="1">MKFLILGKLFCLLAMVSFFGVAFCSDSQEEITEKFYSDTLVKLSDDITAKKLLSLLPNDRFVGNTKAPVVIIEYASFSCMHCAHFTLNVFPELERKYIKTGKVLYIFRNFPLDYVSLKAAMLGTCYNTASRFFTYTRAVFSSIEALVTNYKDLGVLSNIAKISNISDERFEKCINDEDIMNYVIQEKFVANRKLQVNATPVFFINGKKYDKSHDIESFSETIDELIMLHPNYSLVSGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR50</accession>
    <name>Q3YR50_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NMO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304882.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0774"/>
    <dbReference type="EnsemblBacteria" id="AAZ68805">
        <property type="protein sequence ID" value="AAZ68805"/>
        <property type="gene ID" value="Ecaj_0774"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0774"/>
    <dbReference type="eggNOG" id="COG2070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_586452_0_0_5"/>
    <dbReference type="OMA" id="GPIAFQF"/>
    <dbReference type="OrthoDB" id="275153at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMO" evidence="1">
        <location>
            <begin position="15"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="467" mass="52347" checksum="CE667C25D86E58A7" modified="2005-09-27" version="1">MRKKINKVFISGKSVWPIIEGGKGIGVSDGRSAGAFAAASAVGTFSGACARLIDDNGEHIPLIYRGKTRLERHNELINYSIDAAVSQAKMAHEISKGLGRIHMNVLWEMGGVQRVLHGVLEKAKGLIHGITCGAGMPYKLAEIASQYQVYYYPIVSSMRAFKILWQRSYQKFSKTLLGGVVYEDPWLAGGHNGLSNSESPDNPQDPFERVSAIRTYMNEVGLSDVVLIMAGGVWHLKDWEEWLDNSLIGPIAFQFGTRPLLTQESPISAEWKKKLMSLKPGDVFLNKFSPTGFYSSAVKNEFIKELQGRNERQIAFENEMTEKCSAELSIGSRGRKVYVHPYDKKLSESWIAMGYTDALKTPDNTLIFVTQSKSRSIREDQINCMGCLSHCKFSNWKDHGDYNTGIKPDVRSFCIQKTLQNIVAGADHEHELMFSGHNAYKFVQDEFYKDGYIPTVQELVDRILTGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR51</accession>
    <name>Q3YR51_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lumazine-binding protein</fullName>
            <ecNumber evidence="4">2.5.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0773</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0773"/>
    <dbReference type="EnsemblBacteria" id="AAZ68804">
        <property type="protein sequence ID" value="AAZ68804"/>
        <property type="gene ID" value="Ecaj_0773"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0773"/>
    <dbReference type="eggNOG" id="COG0307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034388_2_2_5"/>
    <dbReference type="OMA" id="HFVTGHV"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="PTHR21098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000498">
        <property type="entry name" value="Riboflavin_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="11"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="3">
        <location>
            <begin position="11"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="3">
        <location>
            <begin position="110"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="110"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="212" mass="23627" checksum="D058480391A415EE" modified="2005-09-27" version="1">MVNSIFYTCNMFKGIVEDIGTVIQVRNTKNCDRRVSIKPSNFKFLSETKLGDSIGCSGICLSVVELNDDYFVTDVSDATLSITNATYWEEGTKVNLELPIRITDRLDGHFVQGHVDGIGKITSIIKVNSSHNIELHVPDTLLKYTITKGSIAINGVSLTINSISNGILSVNIIPHTWEKTTFQYSKVNDIINIEADILAKHLEQLYIYNIKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-29" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR62</accession>
    <name>RNH_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease H</fullName>
            <shortName evidence="1">RNase H</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhA</name>
        <name type="ordered locus">Ecaj_0761</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304870.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YR62"/>
    <dbReference type="SMR" id="Q3YR62"/>
    <dbReference type="STRING" id="269484.Ecaj_0761"/>
    <dbReference type="EnsemblBacteria" id="AAZ68793">
        <property type="protein sequence ID" value="AAZ68793"/>
        <property type="gene ID" value="Ecaj_0761"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0761"/>
    <dbReference type="eggNOG" id="COG0328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030894_6_0_5"/>
    <dbReference type="OMA" id="MQEIEIF"/>
    <dbReference type="OrthoDB" id="1953872at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09278">
        <property type="entry name" value="RNase_HI_prokaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00042">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022892">
        <property type="entry name" value="RNaseHI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000332594" description="Ribonuclease H">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="4"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00408"/>
        </source>
    </evidence>
    <sequence length="146" mass="16550" checksum="386A18EDAAFAFAC4" modified="2005-09-27" version="1">MKDELNKVVIYTDGACSGNPGPGGWGAILLFDKNERTICGNNPDTTNNRMELTAVIEALKFLKVAYNVDLYTDSIYVKDGITLWIEKWKINGWRTASKLPVKNLELWLELDSLASFHNVTWYWVKAHAGNLYNQKADILARSQISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR67</accession>
    <name>Q3YR67_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0756</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304865.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68788">
        <property type="protein sequence ID" value="AAZ68788"/>
        <property type="gene ID" value="Ecaj_0756"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0756"/>
    <dbReference type="HOGENOM" id="CLU_813132_0_0_5"/>
    <dbReference type="OMA" id="LASCACD"/>
    <dbReference type="OrthoDB" id="1116536at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="332" mass="38890" checksum="090F7B515C22DD05" modified="2005-09-27" version="1">MKSISTLLYSYLCSELSKMTEELYNTDRVKLSENPELCKPLTPDEKALVRLANYSGNLLVDPMMIEYMNLDDKIVKDIRSKILWITESLSTIHNNIVYIYSTDDVNQKFDIMLSSELLFDEINAEAEDIRYLCGELIKYSNVIIKEDFFHICFAIHNINFFTHNVQEHVKNMRRIMFMRNKVRDVDDISVPIERRVNFDDDTYLCGLKDKDKSGLMILYKDFHDYFEREYGYNMLILFESVIRCDTPNEKVFPQGSQIVVLNSREVAYIRCINNIVNFIALYDKSRITLVEGTNNVLVYVNLVDQEIDYDVSDSDDELHNVAINCNDTLRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR75</accession>
    <name>Q3YR75_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304857.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68780">
        <property type="protein sequence ID" value="AAZ68780"/>
        <property type="gene ID" value="Ecaj_0748"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0748"/>
    <dbReference type="HOGENOM" id="CLU_2034397_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="121" mass="13655" checksum="1EB441918230545A" modified="2005-09-27" version="1">MYRDIFCIIVAVAVVLVILILLCIIGYMISCIKSLGNQIDKMKTELSQCRKEDTEMMEEVLTLYDEVYDMRNDLAYHPYFGNVKNGKKLGNEVGDTTLQQGSCTLDSTNVKGLGDKCEKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRA5</accession>
    <name>Q3YRA5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304827.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68750">
        <property type="protein sequence ID" value="AAZ68750"/>
        <property type="gene ID" value="Ecaj_0718"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0718"/>
    <dbReference type="HOGENOM" id="CLU_1084768_0_0_5"/>
    <dbReference type="OrthoDB" id="2139676at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="256" mass="29813" checksum="CDE3B0D19C7EF1EA" modified="2005-09-27" version="1">MHVNVIHAIVSLLVFTLVVFCLLLCCLLRYFCSLKHNEHLLKQQKQEFYKKLSEAVGEEGDIKLVSTRRKIYEPLRPTGRRLRVPKKLCTGNTATKFKYLLRVSSTKFDDIPFLRCLMLRAYYAMVFHDMLVAMLLWGRGMSLVNTEKIISHIARFSAYFYDTQWLAASILGNPSLLANEKILQFIKMGESILKQAVEGLKELLIKAYSDDVETLKFYQRKVAHFMKHCEREERISSSFEGVEYSVKGLYLLPRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRA6</accession>
    <name>Q3YRA6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304826.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68749">
        <property type="protein sequence ID" value="AAZ68749"/>
        <property type="gene ID" value="Ecaj_0717"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0717"/>
    <dbReference type="HOGENOM" id="CLU_1223170_0_0_5"/>
    <dbReference type="OrthoDB" id="2165365at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="226" mass="25284" checksum="47BE4D6E297B385A" modified="2005-09-27" version="1">MLGDEIAAIVIGVLLIIVSAILLYRLICWMLTPELHVKKGIPSHIDVQQTKFDSEETKPQTVTLPKVRTAGYLQFMLEAHDCCFNIVLDMMKQSANNKMLVGDNLTFLMDKICNSSSKLKELQNLSIGSEAALEQYLPTIYQCCLALVKYGSLIQSQIKKVSRKTCNTEQLIPYQLTVSVLMHECNMLLREIKQEMVNATESDRLPVSILESSTANSHSTSTGLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRB5</accession>
    <name>Q3YRB5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0708</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of an ABC transporter complex. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304817.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0708"/>
    <dbReference type="EnsemblBacteria" id="AAZ68740">
        <property type="protein sequence ID" value="AAZ68740"/>
        <property type="gene ID" value="Ecaj_0708"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0708"/>
    <dbReference type="eggNOG" id="COG4133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_5"/>
    <dbReference type="OMA" id="VIITTHE"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005895">
        <property type="entry name" value="ABC_transptr_haem_export_CcmA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43499">
        <property type="entry name" value="PTHR43499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01189">
        <property type="entry name" value="ccmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="2"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="210" mass="23870" checksum="E4BD7F1C53B6C453" modified="2005-09-27" version="1">MLECKDLACIRNNKCLFSNLNFTTQQNLLTLVIGNNGSGKTSLLRLLAGLIPITHGSVKYNGKNIHNSDTYFASLTYIGHKNFCNDNLTVLENIQLWANLKNTNELIAATICFFELQLVLDIKYKDLSSGWKRRVALSRLLIYNTNIWIIDEPFVHLDIHIKELILELISTRLKQKNSIVIISDHSKAAIYGECQVIDLNISKSQQSNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRC3</accession>
    <name>Q3YRC3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BolA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0700"/>
    <dbReference type="EnsemblBacteria" id="AAZ68732">
        <property type="protein sequence ID" value="AAZ68732"/>
        <property type="gene ID" value="Ecaj_0700"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0700"/>
    <dbReference type="eggNOG" id="COG0271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_4_2_5"/>
    <dbReference type="OMA" id="DDDHYHL"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="SSF82657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="76" mass="8672" checksum="6C92D3359D2F89FF" modified="2005-09-27" version="1">MLKMTIIQSQLETLIKDAFPEAEVTITSLVNDNNHYSIKVISNQFQGKSKLEQHRMIYKVLDGLNIHAIQIQTGCK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRE0</accession>
    <name>RUVA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Holliday junction ATP-dependent DNA helicase RuvA</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvA</name>
        <name type="ordered locus">Ecaj_0683</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with RuvB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304792.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRE0"/>
    <dbReference type="SMR" id="Q3YRE0"/>
    <dbReference type="STRING" id="269484.Ecaj_0683"/>
    <dbReference type="EnsemblBacteria" id="AAZ68715">
        <property type="protein sequence ID" value="AAZ68715"/>
        <property type="gene ID" value="Ecaj_0683"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0683"/>
    <dbReference type="eggNOG" id="COG0632">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087936_3_0_5"/>
    <dbReference type="OMA" id="VGMAVQC"/>
    <dbReference type="OrthoDB" id="1370858at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009379">
        <property type="term" value="C:Holliday junction helicase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14332">
        <property type="entry name" value="UBA_RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00031">
        <property type="entry name" value="DNA_helic_RuvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013849">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvA_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000085">
        <property type="entry name" value="RuvA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011114">
        <property type="entry name" value="RuvA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036267">
        <property type="entry name" value="RuvA_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07499">
        <property type="entry name" value="RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01330">
        <property type="entry name" value="RuvA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46929">
        <property type="entry name" value="SSF46929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000224865" description="Holliday junction ATP-dependent DNA helicase RuvA">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00031"/>
        </source>
    </evidence>
    <sequence length="190" mass="21446" checksum="4458E30D16ABE46D" modified="2005-09-27" version="1">MIGSLTGIIEEIYITYIILNVGNVGYIVHVSQRVLQTCKIGNNIKLYIETHVNRDNLTQLYGFLDKQEQDYMRMLITINGINYKTAMSILSKLSPEQIFSAVVSNNKNAFRGNGIGEKLAGRITTELQYKISKMPIEETLIIKEDDSLAALISLGYDKLKAFNAIQEIKSDFPNANIQEIIRKALQKLSQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRE3</accession>
    <name>FOLD_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein FolD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methylenetetrahydrofolate dehydrogenase</fullName>
                <ecNumber evidence="1">1.5.1.5</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methenyltetrahydrofolate cyclohydrolase</fullName>
                <ecNumber evidence="1">3.5.4.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">folD</name>
        <name type="ordered locus">Ecaj_0680</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,10-methenyltetrahydrofolate + H2O = (6S)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="1"/>
    <dbReference type="EC" id="3.5.4.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304789.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRE3"/>
    <dbReference type="SMR" id="Q3YRE3"/>
    <dbReference type="STRING" id="269484.Ecaj_0680"/>
    <dbReference type="EnsemblBacteria" id="AAZ68712">
        <property type="protein sequence ID" value="AAZ68712"/>
        <property type="gene ID" value="Ecaj_0680"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0680"/>
    <dbReference type="eggNOG" id="COG0190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034045_1_2_5"/>
    <dbReference type="OMA" id="HIVGRPM"/>
    <dbReference type="OrthoDB" id="1449514at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000268340" description="Bifunctional protein FolD">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="168"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01576"/>
        </source>
    </evidence>
    <sequence length="300" mass="32273" checksum="5649AD355D23BEF9" modified="2005-09-27" version="1">MEANIINGKAIAEDITGTLATCINDLKLQYSLVPCLIVVLVGDDPASHLYVRNKQRKAEMLGLRSETILLPSTTSESSLIDKIHELNNDDNVHGILVQLPVPKHIDKNLIINTIDPRKDVDGFHNDNVGRLFTGQKRNCLIPCTPRGCLYLIKTVTHNLSGSDAVVIGRSNIVGKPMACLLLGENCTVTTVHSATRNLSDYCSKADILVAAVGIPNFVKSSWIKPGAIVIDVGINSVEEDGVKKFVGDVNFAEAKNVASAITPVPGGVGPMTIAFLMVNTVMAACNQSGIENFVEKYLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRG7</accession>
    <name>Q3YRG7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0656</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304765.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0656"/>
    <dbReference type="EnsemblBacteria" id="AAZ68688">
        <property type="protein sequence ID" value="AAZ68688"/>
        <property type="gene ID" value="Ecaj_0656"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0656"/>
    <dbReference type="eggNOG" id="COG5388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727539_0_0_5"/>
    <dbReference type="OMA" id="GYICENA"/>
    <dbReference type="OrthoDB" id="1397399at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009922">
        <property type="entry name" value="DUF1457"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07310">
        <property type="entry name" value="PAS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="151" mass="17871" checksum="6C780ADA25C2861F" modified="2005-09-27" version="1">MSEYVSEKRAIKILFSYWNQLRDNRMFPQIEEIEKEEIREIWPHCFIIEVKNDTEDQKYNCNYIGEKAIELYKSDIKVYAEAQNMTLFFPQIIENLFDHLESVIENQEPIMDESETESADGNYIKIRQCLLPLGKNNEVTHIIGVIGGKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRH7</accession>
    <name>Q3YRH7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0644</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68678">
        <property type="protein sequence ID" value="AAZ68678"/>
        <property type="gene ID" value="Ecaj_0644"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0644"/>
    <dbReference type="HOGENOM" id="CLU_2584172_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="80" mass="9482" checksum="0F67D7E833229589" modified="2005-09-27" version="1">MHSVQYTHTIIQYNNLHNQIIITNSLQAYTDILKTHTTIHYAYTHNTHISYNTNISLYFEYTCIKKLNNLYSNLCNQIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRI3</accession>
    <name>Q3YRI3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA/pantothenate metabolism flavoprotein,C-terminal</fullName>
            <ecNumber evidence="2">6.3.2.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.2.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304749.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0638"/>
    <dbReference type="EnsemblBacteria" id="AAZ68672">
        <property type="protein sequence ID" value="AAZ68672"/>
        <property type="gene ID" value="Ecaj_0638"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0638"/>
    <dbReference type="eggNOG" id="COG0452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033319_1_0_5"/>
    <dbReference type="OMA" id="AVTDWCP"/>
    <dbReference type="OrthoDB" id="1346419at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="SSF102645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DFP" evidence="1">
        <location>
            <begin position="3"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="214" mass="24311" checksum="2DB587E5D8C978CF" modified="2005-09-27" version="1">MVNVLITAGPTQEFIDPVRYITNSSSGKQGYAIAEYMNKIGWNVNLVSGPVTIHNHNIQNIFYIKNCNEMLQTCIKLLPVDIAIFTAAVTDWCPIYSAKKIKKHEITNIKITNNPDIIHIISTHAKRPKLVIGFSLESENAIENSKVKLIKKQCDWIISNTTTINDKHVMGNDYNKITIITKNHYQEFPIMSKKDIGKLITEQIINFLANNKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRI4</accession>
    <name>Q3YRI4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fumarate hydratase class II</fullName>
            <shortName evidence="2">Fumarase C</shortName>
            <ecNumber evidence="2">4.2.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Aerobic fumarase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Iron-independent fumarase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fumC</name>
        <name evidence="5" type="ordered locus">Ecaj_0637</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(S)-malate = fumarate + H2O</text>
            <dbReference type="Rhea" id="RHEA:12460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="EC" id="4.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304748.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0637"/>
    <dbReference type="EnsemblBacteria" id="AAZ68671">
        <property type="protein sequence ID" value="AAZ68671"/>
        <property type="gene ID" value="Ecaj_0637"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0637"/>
    <dbReference type="eggNOG" id="COG0114">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021594_4_1_5"/>
    <dbReference type="OMA" id="HDSMGEV"/>
    <dbReference type="OrthoDB" id="734949at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045239">
        <property type="term" value="C:tricarboxylic acid cycle enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006106">
        <property type="term" value="P:fumarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01362">
        <property type="entry name" value="Fumarase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00743">
        <property type="entry name" value="FumaraseC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005677">
        <property type="entry name" value="Fum_hydII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444">
        <property type="entry name" value="PTHR11444"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00979">
        <property type="entry name" value="fumC_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lyase_1" evidence="3">
        <location>
            <begin position="9"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="FumaraseC_C" evidence="4">
        <location>
            <begin position="405"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding (B site)" evidence="2">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="136"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="321"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="2">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="461" mass="49946" checksum="A7B6F184DC34751C" modified="2005-09-27" version="1">MRKERDSLGEIEVPACHYWGAQTQRSINNFKIGSEKMPQPLIKALGLVKLAAARVNMKNGDIDEVIGSAICDAANEVVNGKLNDEFPLVIWQTGSGTQTNMNVNEVIANRAIEMLGGEKGSKCPVHPNDHVNYSQSSNDTFPTAMHIAAVVETENSLLPSLKNLYEALHSKSIAFQNIVKVGRTHLQDATPLTLGQVFSGYAYQILQGMNRIKAALGNLLELAQGGTAVGTGINSRKQFDIHFASEIKKLTGFNFVSSINKFEALATHDALVEFSGVLNVIAVSLMKIANDIRLLGSGPRCGIGEIVLPANEPGSSIMPGKVNPTQCEAMTMVCAQVMGNHTTVTVAGSNGHFELNAFKPVIIYNVLQSIKLLSDVSINFSQKCVQGIKADTKRISFLLNQSLMLVTALNKHIGYDNAAKISKTAFDEGITLKDAAMKLNLISEEEFDRIVNPENMIHPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRI7</accession>
    <name>Q3YRI7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304745.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68668">
        <property type="protein sequence ID" value="AAZ68668"/>
        <property type="gene ID" value="Ecaj_0634"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0634"/>
    <dbReference type="HOGENOM" id="CLU_784666_0_0_5"/>
    <dbReference type="OMA" id="IMICKSA"/>
    <dbReference type="OrthoDB" id="1250284at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="359" mass="40833" checksum="D48AD98F30BB62EE" modified="2005-09-27" version="1">MTVIIKVGIKNLIDSTMEIISERFSVYAKNEVVKTSLQSYIDIIRIKEKKLQYYNGKEAKGHVIADDQFYQMESTIRAILMKIFEIEILEKKTTIPQEKISAYREAVSILINKIVKCNAYVLTENDKLAKIVGNATLRRSTYKCRNLGELVSKSKFTAVESFDAACSNIELDPNIIKQVLEFCSVDLMIGIMDLQLIQFLADMRYFPNQNSNVFERTKHSYMNLIKHIMICKSASAIEQYQSSRDSKKLHCSLYLKDNIETNPALYLVLTLFAIQSIFNMCGLLFVLGRIYNIVNFFNNIDIKDVSIGVCSINVVLCTLIFASILVGSKLSKKNDAIVTDDMVLSNLDIIEQVDESLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRK1</accession>
    <name>Q3YRK1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304731.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0620"/>
    <dbReference type="EnsemblBacteria" id="AAZ68654">
        <property type="protein sequence ID" value="AAZ68654"/>
        <property type="gene ID" value="Ecaj_0620"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0620"/>
    <dbReference type="HOGENOM" id="CLU_2395069_0_0_5"/>
    <dbReference type="OMA" id="CNEVINR"/>
    <dbReference type="OrthoDB" id="1985090at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025310">
        <property type="entry name" value="DUF4164"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13747">
        <property type="entry name" value="DUF4164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="97" mass="11177" checksum="E3C5648651823955" modified="2005-09-27" version="1">MEQTSNFSNSEFQQTIDKAFDKLESSLVQKLSHKNNISQYKADIASLIKEKNTLSQQLADQANECNKWKEACHEVITRLNYTIENVKAILQKEQVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRK2</accession>
    <name>Q3YRK2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone-like bacterial DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0619"/>
    <dbReference type="EnsemblBacteria" id="AAZ68653">
        <property type="protein sequence ID" value="AAZ68653"/>
        <property type="gene ID" value="Ecaj_0619"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0619"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_2_1_5"/>
    <dbReference type="OMA" id="YFKTYFR"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="87" mass="10314" checksum="424B0BCE418B068A" modified="2005-09-27" version="1">MLKSHLVQHISKKNPSLSEEIVSKILNIFFSLIIEYLKNGYRVELRGFGSFTVRSYNIKTVNTRLTKNKYRKVYFRASNKFIKLINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRK5</accession>
    <name>Q3YRK5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytidylate kinase</fullName>
            <shortName evidence="8">CK</shortName>
            <ecNumber evidence="8">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cytidine monophosphate kinase</fullName>
            <shortName evidence="8">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cmk</name>
        <name evidence="10" type="ordered locus">Ecaj_0616</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + CMP = ADP + CDP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>ATP + dCMP = ADP + dCDP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the cytidylate kinase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304727.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0616"/>
    <dbReference type="EnsemblBacteria" id="AAZ68650">
        <property type="protein sequence ID" value="AAZ68650"/>
        <property type="gene ID" value="Ecaj_0616"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0616"/>
    <dbReference type="eggNOG" id="COG0283">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079959_0_1_5"/>
    <dbReference type="OMA" id="RAITWWM"/>
    <dbReference type="OrthoDB" id="776861at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00238">
        <property type="entry name" value="Cytidyl_kinase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003136">
        <property type="entry name" value="Cytidylate_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02224">
        <property type="entry name" value="Cytidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00017">
        <property type="entry name" value="cmk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidylate_kin" evidence="9">
        <location>
            <begin position="10"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="14"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="214" mass="24616" checksum="262B8B4225FD7915" modified="2005-09-27" version="1">MTKHSNNFVITIDGPCASGKGSLATKLSSFFNFQYLDTGKLYRIIASIFGTSAQYNLNIDTHLEEKILSLLLEDSNAASYHNEDIGKTASILARHKHIRLALLPIQRKIIYSAKPGIILDGRDTSSIIFPDSDFKIFITAQPSIRAQRRFKEMLHKKKAMYKKIHQQIIQRDIRDTYRKFAPLIKVKDALYVNTSYMNEEQVFLLIKEKIKTYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRK8</accession>
    <name>RS10_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsJ</name>
        <name type="ordered locus">Ecaj_0613</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304725.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRK8"/>
    <dbReference type="SMR" id="Q3YRK8"/>
    <dbReference type="STRING" id="269484.Ecaj_0613"/>
    <dbReference type="EnsemblBacteria" id="AAZ68647">
        <property type="protein sequence ID" value="AAZ68647"/>
        <property type="gene ID" value="Ecaj_0613"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0613"/>
    <dbReference type="eggNOG" id="COG0051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122625_1_3_5"/>
    <dbReference type="OMA" id="QFEIRVH"/>
    <dbReference type="OrthoDB" id="2012682at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_S10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_S10_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="PTHR11700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="SSF54999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01049">
        <property type="entry name" value="rpsJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000237040" description="30S ribosomal protein S10">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <sequence length="108" mass="12135" checksum="F1155AB90B91115A" modified="2005-09-27" version="1">MVTQKIYIELKAFDSCLLDRSARSIILTAKRSGARVNGPIFFPRKVMKFIVNRSTHVDKKSREQFEIRTHKRLISLPRANSTIIQALMSLQLPAGVDVKVKVIGGNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRM4</accession>
    <name>Q3YRM4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0597</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304709.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0597"/>
    <dbReference type="EnsemblBacteria" id="AAZ68631">
        <property type="protein sequence ID" value="AAZ68631"/>
        <property type="gene ID" value="Ecaj_0597"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0597"/>
    <dbReference type="eggNOG" id="COG0097">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_1_2_5"/>
    <dbReference type="OMA" id="KPDPYKG"/>
    <dbReference type="OrthoDB" id="1398618at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_L6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_L6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_L6_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019906">
        <property type="entry name" value="Ribosomal_L6_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="PTHR11655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF14">
        <property type="entry name" value="PTHR11655:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00059">
        <property type="entry name" value="RIBOSOMALL6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="SSF56053"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03654">
        <property type="entry name" value="L6_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L6" evidence="5">
        <location>
            <begin position="11"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L6" evidence="5">
        <location>
            <begin position="91"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="178" mass="19824" checksum="F46D74BD41FF7A22" modified="2005-09-27" version="1">MSRVGSMPIAIPSNVQVDLVGNQLILKNSENSLKLNLNHNVLCDITDGKIVLRVNDKSIKSKVIWGTYRSIINNYAIGLSQGVVVELEVNGVGYKAEINGEYLSLFLGFSHGIKYKIPKGIVIQCVKPTQILVKGFDKQKVYMVASDICSIRKYNPYKGKGISIKGKYVHRKEVNKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRM6</accession>
    <name>RS5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsE</name>
        <name type="ordered locus">Ecaj_0595</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S4 and S8.</text>
    </comment>
    <comment type="domain">
        <text>The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304707.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRM6"/>
    <dbReference type="SMR" id="Q3YRM6"/>
    <dbReference type="STRING" id="269484.Ecaj_0595"/>
    <dbReference type="EnsemblBacteria" id="AAZ68629">
        <property type="protein sequence ID" value="AAZ68629"/>
        <property type="gene ID" value="Ecaj_0595"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0595"/>
    <dbReference type="eggNOG" id="COG0098">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_2_2_5"/>
    <dbReference type="OMA" id="KRGCGSW"/>
    <dbReference type="OrthoDB" id="1505038at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_B">
        <property type="entry name" value="Ribosomal_S5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_S5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005712">
        <property type="entry name" value="Ribosomal_S5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_S5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="PTHR13718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01021">
        <property type="entry name" value="rpsE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000230345" description="30S ribosomal protein S5">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="S5 DRBM" evidence="1">
        <location>
            <begin position="16"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <sequence length="174" mass="18928" checksum="D3879B7DDF8AA159" modified="2005-09-27" version="1">MDVVKKSRNVHGANDFSELIVSVRRVAKVVKGGRRFSFSVLVVIGDEKGKVGCGIGKHLEVSEAKVKAVNAAKKNMIRVHLRESRTLHHDVKAKFCASKVMLRSAKVGTGIIAGGSIRLIFEVLGVQDVVAKSIGSSNPHNVVYAVFAAFKKMLSPKQVANKRSRKIGDIIENR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRN4</accession>
    <name>SYFB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheT</name>
        <name type="ordered locus">Ecaj_0587</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304699.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRN4"/>
    <dbReference type="SMR" id="Q3YRN4"/>
    <dbReference type="STRING" id="269484.Ecaj_0587"/>
    <dbReference type="EnsemblBacteria" id="AAZ68621">
        <property type="protein sequence ID" value="AAZ68621"/>
        <property type="gene ID" value="Ecaj_0587"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0587"/>
    <dbReference type="eggNOG" id="COG0072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016891_0_0_5"/>
    <dbReference type="OMA" id="ISYNWLK"/>
    <dbReference type="OrthoDB" id="53568at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02796">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00283">
        <property type="entry name" value="Phe_tRNA_synth_beta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004532">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033714">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PTHR10947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="SSF54991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00472">
        <property type="entry name" value="pheT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000232061" description="Phenylalanine--tRNA ligase beta subunit">
        <location>
            <begin position="1"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-binding" evidence="1">
        <location>
            <begin position="38"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="1">
        <location>
            <begin position="394"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="1">
        <location>
            <begin position="690"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00283"/>
        </source>
    </evidence>
    <sequence length="785" mass="88101" checksum="A97C1528FB7E860C" modified="2005-09-27" version="1">MKFTFSWLRRYLDTDVSLDVIIKKLSDIGIEVNNVDYCEHLKTFVIAEVLEVNPHHSANKLKICKVSDGKQILQVVCGASNVKVGMKSVLACVGSIMPTDQSIIEVVKLRGVESYGMLCSNDELGIVDHRNSKNGIIELPNTYNVGDTFFPCDPIIEVSITPNRGDCLGVYGIARDLAAAGLGKLKCIGELSNHSNCSSPIEVSVQVDGIVKGRYIKGIKNCESPKWLKDYLLVCGINSISFVVDITNYIMLSFNRPLHVYDANKIKNKLIFKKADNQTEFYALNDKKYILGTENIVAVDLENSIHSVAGVIGSKLSSCLLDTEDIFLESAWFAPVDIVLSSKKIKLSTDSSYRFERFVDPRFLQTGLDLATKMILEYCGGTHFDVVSNEVHVVDNIELNFFPDSVRSIGNVNISNEEIFDFLIKLGFIVDNNNEFLWRVTVPSWRSDIKHSSDIVEEVLRLYGYDKICEEPIPISHVDISDDYHDRLKALLLSEGLMEVITWSFTNMVFAEKLGYSSELLLIDNPISDKLNLMRPSLLLNLLQVVYENQAYGSSEIAIFEIGQVYGFNNICGSNSNVVCGVRYGNNLPRNLYKCDRSVDVFDVKSDVISILQELNIESNSIELRKSDKSYLHPVRSADVYFKDICLGYFGELHPNIVHLYEIKRPVVCFEVFLYKIPKVDVAHKELTESCYQSVKRDFAFLIDKDTSVQHLIDVTKNSNPVLIDNVTVFDLYEGNSIGDNKLSVALSVTFNPVDHTLNDQEIKDASDLIINAVAEKLGGVLRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRP1</accession>
    <name>Q3YRP1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavin reductase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304692.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0580"/>
    <dbReference type="EnsemblBacteria" id="AAZ68614">
        <property type="protein sequence ID" value="AAZ68614"/>
        <property type="gene ID" value="Ecaj_0580"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0580"/>
    <dbReference type="eggNOG" id="COG1853">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059021_1_0_5"/>
    <dbReference type="OMA" id="RFAMKGR"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="1">
        <location>
            <begin position="14"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="163" mass="18071" checksum="40921A17F58CBEEF" modified="2005-09-27" version="1">MMGGISKAYLKSCLSEFVTGITIITTVSESGSMHGITVNSFNSVSLVPPMVLFSIEKSASRFEIFHSCSRFIVNILSEKQKDVSKSFAERYVQDWGDFSSNVIDGIPVIDGVMSYIYCTKKHVYDGGDHKIIVGEVIDCSKLNNEDRPLVYYKGKYMIIGGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRT4</accession>
    <name>Q3YRT4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Superoxide dismutase</fullName>
            <ecNumber evidence="8">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ecaj_0535</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Catalyzes the dismutation of superoxide anion radicals into O2 and H2O2 by successive reduction and oxidation of the transition metal ion at the active site.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2 H(+) + 2 superoxide = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:20697"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304649.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0535"/>
    <dbReference type="EnsemblBacteria" id="AAZ68571">
        <property type="protein sequence ID" value="AAZ68571"/>
        <property type="gene ID" value="Ecaj_0535"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0535"/>
    <dbReference type="eggNOG" id="COG0605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031625_0_0_5"/>
    <dbReference type="OMA" id="KWGSFDK"/>
    <dbReference type="OrthoDB" id="1440645at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="SSF46609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="SSF54719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sod_Fe_N" evidence="9">
        <location>
            <begin position="2"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Sod_Fe_C" evidence="10">
        <location>
            <begin position="96"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="205" mass="23533" checksum="403012E6600512DC" modified="2005-09-27" version="1">MFTLPELPYQQSDLVPYLSPEILGYHYNKHHQGYVNTLNSLVVGTDFSECTNEDLPKIIEATAGDLGSRSIFNNAGQIWNHNFYWESMKKNGGGAPTGKLLDKINEDFGSIDDFNNAFTNAGKSHFGSGWVWLVFDMSEQKLKILCTSNGDTPITQYPETHPLLTMDVWEHAYYLDYFNVRQNYVETFLKHLVNWDFAAQRFLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRT6</accession>
    <name>Q3YRT6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type IV secretion system protein virB4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0533</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrbE/VirB4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304647.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YRT6"/>
    <dbReference type="STRING" id="269484.Ecaj_0533"/>
    <dbReference type="EnsemblBacteria" id="AAZ68569">
        <property type="protein sequence ID" value="AAZ68569"/>
        <property type="gene ID" value="Ecaj_0533"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0533"/>
    <dbReference type="eggNOG" id="COG3451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008341_3_0_5"/>
    <dbReference type="OMA" id="MFMDEFW"/>
    <dbReference type="OrthoDB" id="182908at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004346">
        <property type="entry name" value="CagE_TrbE_VirB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018145">
        <property type="entry name" value="CagE_TrbE_VirB_cntrl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043964">
        <property type="entry name" value="P-loop_TraG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03135">
        <property type="entry name" value="CagE_TrbE_VirB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19044">
        <property type="entry name" value="P-loop_TraG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00929">
        <property type="entry name" value="VirB4_CagE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0843">Virulence</keyword>
    <feature type="domain" description="AAA" evidence="4">
        <location>
            <begin position="445"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="800" mass="90856" checksum="0F67960B3F965848" modified="2005-09-27" version="1">MLKFQQLQTKRRKVINREYHESNFIPYSEHWNSSTLMTKDGSMLKVIKLSGYAFETADDEDLSIQNSIRNQMLRSISSASFGLYFHIIRRRRNAFSEGFASDKVASSFANYVNVKWREKHETKQSFTNDLYITIVRESGKQGVEFLSSIFGKFGKVASQESWIKDLQAIYEDLDEVTNRVFTSLRNYMPRVLGIRETPKGVFSEIMEFLLQIVNCGSFQRVLFSIGDVSKYLPMHRLYFGHKTIQIVAHNECKYAGLISLKEYGQSTSAGMLDAFLQLPYEFIITQSFSFTNRQSAITKMQIQQNRMIQSADKAVSQVVEISQALDDAMSGRIAFGQHHLTILCTERSLKTLDNALSLIESELSNCGVYPVRERINLEPAFWAQLPGNFEYIVRKAIISTLNMAGFASQHNYPIGKKFNNHWGEAVTVFDTTSGTPFFFNFHIRDVGHTAIIGPTGGGKTVLMNFLCAQAMKFSPRIFFFDKDYGAEIFIRALSGVYMIVEPRNPTGFNPLQLDDTPDNRTFLMEWMKSLISVFNDKFTSEDITRINDAIEGNFKLKKEHRVLCNLVPFLGLDGPNTLAGSISMWHSKGSHAAIFDNAEDLLDFNKSRVFGFEMGHLLKDPVSLAPVLLYLFHRISISLDGTPSIIVLDEAWALIDNPVFAPKIKDWLKVLRKLNTFVIFATQSVEDASKSAISDTLVQQTATQIFLPNLKATSAYRNVFMLTEREYTLIKHTDPSTRFFLVKQGVSAVVARINLSGLEDVISVLSGRAETVLMLRDLIKEVGDDPNTWLPIFYERVKHV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRU2</accession>
    <name>DUT_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="1">dUTPase</shortName>
            <ecNumber evidence="1">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dut</name>
        <name type="ordered locus">Ecaj_0527</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dUTP + H2O = diphosphate + dUMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dUTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304641.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRU2"/>
    <dbReference type="SMR" id="Q3YRU2"/>
    <dbReference type="STRING" id="269484.Ecaj_0527"/>
    <dbReference type="EnsemblBacteria" id="AAZ68563">
        <property type="protein sequence ID" value="AAZ68563"/>
        <property type="gene ID" value="Ecaj_0527"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0527"/>
    <dbReference type="eggNOG" id="COG0756">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068508_1_2_5"/>
    <dbReference type="OMA" id="YAAFVHP"/>
    <dbReference type="OrthoDB" id="1669228at2"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00116">
        <property type="entry name" value="dUTPase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="PTHR11241"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00576">
        <property type="entry name" value="dut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000231409" description="Deoxyuridine 5'-triphosphate nucleotidohydrolase">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00116"/>
        </source>
    </evidence>
    <sequence length="153" mass="16556" checksum="16AA6DA9200C449C" modified="2005-09-27" version="1">MKNNTDILIKVIKLNSNNLPLPSYSTENSSGMDLYSAMTQDVILAPGCRACINTGIAISVPNGYEAQVRPRSGLALKFGITVLNTPGTIDADYRGEIKVILINLGHETYTIKYGDRIAQMVIAPVIHASWNLVKDLDDDTTKRGDQGFGSTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRV0</accession>
    <name>Q3YRV0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bacterial translation initiation factor 1 (BIF-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0519</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304633.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0519"/>
    <dbReference type="EnsemblBacteria" id="AAZ68555">
        <property type="protein sequence ID" value="AAZ68555"/>
        <property type="gene ID" value="Ecaj_0519"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0519"/>
    <dbReference type="eggNOG" id="COG0361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151267_0_1_5"/>
    <dbReference type="OMA" id="AHVSGKM"/>
    <dbReference type="OrthoDB" id="2066663at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004368">
        <property type="entry name" value="TIF_IF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33370">
        <property type="entry name" value="PTHR33370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00008">
        <property type="entry name" value="infA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="82" mass="9248" checksum="30DAAFFFFADDB178" modified="2005-09-27" version="1">MVTKEGIIEAEGIVVKLLRDATFMVKLNDNYEIIAHASGKIRKSKIRILMHDRVLVEISTYGINKGEVGKGRIIRRLKITDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRW1</accession>
    <name>Q3YRW1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304622.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68544">
        <property type="protein sequence ID" value="AAZ68544"/>
        <property type="gene ID" value="Ecaj_0508"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0508"/>
    <dbReference type="eggNOG" id="COG3064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026149_0_0_5"/>
    <dbReference type="OMA" id="KCKLFGN"/>
    <dbReference type="OrthoDB" id="373711at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230941" evidence="1">
        <location>
            <begin position="24"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="651" mass="72508" checksum="FE247146EDB1CEFF" modified="2005-09-27" version="1">MRLTLVSSALASFFVLLGSSAFSVDILSDVNIFNLDAENKQVDKVNEKNLSKNLENKERNNLVKVGRALVSGQAISYMWASADKCDDIFDISGNMDNYGMSYDALLRLGAEIRSGDAGTKYGADFQVAIPSVKGRNFERKAALNRGSRIFAATQYGNFSVGYQEGVESIMKLDSSNIIAGDESSSWTQHLRGALSEKKNALGYPMYPFLFSAGLYSENVFRNDDNIELAANNKDFINNLPLRMSYLSPSFMGLRFGVSYSPLGYKFDSFSTVLEQYTTEEISVMTKMPELSVDSSSGTVDLTELISALQGGGGLGENCELKLSNDDMYLEINLPEYGDDQQGVRCKVIGPTTSLVEGSDFTFYIDLQENENKKAKVPISNLKLCNAKVNFEGLENLQKPTFELGKLKITGAENVSKSPLIKRTKSKEDSVLFGVKYEHIFSGSIAYDYDFHDFKFSTSIVGEYARPRLYFNTKNYNIYPESYNLQGVSIGSVLSYKNVNFAAAYGYLGRSGFVKYYSVLNNTADSTTTVSVQPLYERSNTYYLDAALGYQYHSYYISVGYFKSSRSGNILQDINLGIEYNLLKSQSKMKCKLFGNYHYYKFSEVSIVDKVCYNSEVGEPACSVCGLAEHRLEKHEKSGFGNIFLVGAKLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRW5</accession>
    <name>Q3YRW5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribonuclease BN</fullName>
            <ecNumber evidence="4">3.1.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0504</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304618.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68540">
        <property type="protein sequence ID" value="AAZ68540"/>
        <property type="gene ID" value="Ecaj_0504"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0504"/>
    <dbReference type="eggNOG" id="COG1295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045539_4_3_5"/>
    <dbReference type="OMA" id="KQPKFRW"/>
    <dbReference type="OrthoDB" id="1421045at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017039">
        <property type="entry name" value="Virul_fac_BrkB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30213">
        <property type="entry name" value="PTHR30213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03631">
        <property type="entry name" value="Virul_fac_BrkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035875">
        <property type="entry name" value="RNase_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00765">
        <property type="entry name" value="yihY_not_rbn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="279" mass="31895" checksum="7B44BA6565CA0600" modified="2005-09-27" version="1">MLIKVARCFIKCVYYAMVDMVKHDGIEHAGYLSFIILLSIFPFLVFLMALVSNVAQLLNYYDISISEKLILLINDNVPSEIISGIMPRIQEIISGPPHSLLTLAIIGTVWTSSSAVEGIRTILNKAYRVSSPPSYIFRRLLSIFQFLMITVTITIAMVSIILLPVIYEMLDRNLAYFKYAISELMLFTSITLLYFIVPNIKQNLINVVPGAMLVTFLWTCSSFVFTWYIKTFQQLNIIYGSLAGIIASLLFFYILSIFFIYGAEFNYRLKHALMYNLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRW6</accession>
    <name>Q3YRW6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0503</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304617.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68539">
        <property type="protein sequence ID" value="AAZ68539"/>
        <property type="gene ID" value="Ecaj_0503"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0503"/>
    <dbReference type="HOGENOM" id="CLU_2166988_0_0_5"/>
    <dbReference type="OrthoDB" id="2172627at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="110" mass="11457" checksum="DC74D15FD407F9CE" modified="2005-09-27" version="1">MKNSKFQSALLNTIGAFSTVMALFSGVATGITIVGMLSNAKIPGTDPSIGSSLALLAATAIFSLIAIGSLLGSRALPIFLQPNTTEQTPNPELTDSNDHTPLSRTPNHQY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRX1</accession>
    <name>SYR_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="1">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argS</name>
        <name type="ordered locus">Ecaj_0498</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304612.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRX1"/>
    <dbReference type="SMR" id="Q3YRX1"/>
    <dbReference type="STRING" id="269484.Ecaj_0498"/>
    <dbReference type="EnsemblBacteria" id="AAZ68534">
        <property type="protein sequence ID" value="AAZ68534"/>
        <property type="gene ID" value="Ecaj_0498"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0498"/>
    <dbReference type="eggNOG" id="COG0018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_0_1_5"/>
    <dbReference type="OMA" id="NKPLHLG"/>
    <dbReference type="OrthoDB" id="1146366at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000242017" description="Arginine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <sequence length="576" mass="65487" checksum="EECF47FF2801758D" modified="2005-09-27" version="1">MNLINTIKDCIVERLHVLNAKKLIILDDVILSKLVVDYPNNHDYGDLYTNAALILSKYVKKDPLDVAEILLRELSNIKEISNISVVKPGFINFNLSLYVWYEVISSINMLKEDFANVNIGNGRKVNIEFVSANPTGPMHIGHARGAIFGDVLANLLTKVGYQVVREYYINDAGTQIDVLVESVYLRYKEAAGQDIVIGSGLYPGLYLKEIGQLLYEKYGTDLLEMNSAQKMKIIRDISLKHLLDLIKQDLVLLGIKHDVFTSEAELLRNNVVEKCVKLLEDKKLIYYGVLDQPKGTENNNWQPRKQMLFKSTDFGDDVDRALQKVDGSWTYFASDIAYHFDKISRGFQHMILELGSDHIGYVKRLKAAVKALSDNTATIDIKLHNIVNFLNNGVQVKMSKRSGEFLTIKDVIEEVGIDVVRFIMLTRKSDAVLDFDFVKVVEQSKNNPIFYVQYAHARVCSLMRNAPNILKIEDTDFSVLSSKEEILLIKLLAKWSNVIELSAKTAEPHRITFYLIEVAETFHMLWGYGNKDVNRRFIIDDDINLTSARIYLARAVAYVISSGLKIFSIVPLEEMK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRX3</accession>
    <name>SYI_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="1">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ileS</name>
        <name type="ordered locus">Ecaj_0495</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRX3"/>
    <dbReference type="SMR" id="Q3YRX3"/>
    <dbReference type="STRING" id="269484.Ecaj_0495"/>
    <dbReference type="EnsemblBacteria" id="AAZ68532">
        <property type="protein sequence ID" value="AAZ68532"/>
        <property type="gene ID" value="Ecaj_0495"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0495"/>
    <dbReference type="eggNOG" id="COG0060">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_1_1_5"/>
    <dbReference type="OMA" id="KMMAPFT"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="PTHR42780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000098543" description="Isoleucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="647"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="650"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02003"/>
        </source>
    </evidence>
    <sequence length="1120" mass="131115" checksum="E982615B4B75549D" modified="2005-09-27" version="1">MCIVKYLSLIINTMTEHYSQLTGEPDFPSIEENVLKFWQENNIFKKSVDNRDENKRFIFYDGPPFANGLPHYGHLLTGFIKDTVARYKTMAGFKVERRFGWDCHGLPAEMLSEKELGISGKLAIEKFGIEKFNNHCRNSVMKFSKEWKQYVDRQARWVDFENDYKTMNSSFMESIIWSFHELWNKGLIYESIKIVPYSWACQTPLSNFETRMDNAYREKTSKTVTVAFELLESPKFITVENVKTYKILVWTTTPWTLPCNLALAISKNIKYCGAIIKHEMLIFATGYLKIFQEHCKKNNIEYQLYNQDISSVNLEDLHYKPLFKYFADVKNAFKILTADFVVEGEGTGIVHIAPGFGEDDFILCKMQDIPHIEGDTSNLLSIICPIDDGAKFTDKISDFKNMHVFDTNDQIINILKQKNLCFKIDQYLHNYPHCWRTDTPLIYRAMSSWYVEVTKIKDKMIELNKTVNWIPNHICNGQFGKWLENAKDWAISRNRFWGTPLPVWKSDNPNYPRIDVYGSIRKVFNNVKALEEDFDISSINDLHRPYIDNLVRPNPDDPTGKSMMRRVSDVFDCWFESGSMPYAQLHYPFENKEFFENYFPADFITEYIAQTRGWFYTLFILSTALFNKPPFINCICHGVVLDTQGQKLSKRLNNYADPMEIFNQYGSDAMRFLMLSHTVLYGGDLLLDKEGVMIKDVLRNVIKPIWNSYNFFTIYANIDHITAEIITELNELSNIMDRYIICECINTIHSIFNAMEELDQCSNNLGYNIKLACNNITKFFEILNNWYIRRCRSRFWSSEITQDKQDAYNTLYTVIYYMIKVSAPFLPIITEAIWQRLNFQKEESVHLSSLPNISNFILNNEDKQNIQYMKLITCICNYVLSIRSTHNIRIRQPLNKIVIYSHNCPDLLNLPAEYQNILLEEVNVKSISFKSDISDIASFQLKLNFPELGKRIPDKVKRLIFLLKNDQWKILENDKLLLGTIEAEHYVINNNEYTLALKVHNDFACTINLDQHLLGVVLLDNELSNELIMEGIARDIIRTIQHSRKDNKFNISDKIDVIIHTKDNIVKDSIKTWSQYIIQQTLSTSFAIHEEISDIQDINEYYKTTMKDKEVSVFLKKSHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRY8</accession>
    <name>Q3YRY8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68517">
        <property type="protein sequence ID" value="AAZ68517"/>
        <property type="gene ID" value="Ecaj_0480"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0480"/>
    <dbReference type="HOGENOM" id="CLU_118039_0_0_5"/>
    <dbReference type="OrthoDB" id="2010142at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="205" mass="23694" checksum="87E94545074D48EA" modified="2005-09-27" version="1">MIYHFRKNHTWINHMQLIFLFKDNVPTLVPKSACKYNKALDIYESQIPGLMLTVPAERYNPEFQFRYLYKAVFDRYCVATNVVLGYNIIFLFGKSSDGIGQYVVCSIRDGDLRNVIAYGLMLRPGTIISAGSMTTTRSLEDHTFNLFEILYKNIQLTSDKQITRQFRIDFFNDQGDCFGYRCKGTTLNSVSVDYLGNTVYVMNLT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRZ5</accession>
    <name>DXR_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-deoxy-D-xylulose 5-phosphate reductoisomerase</fullName>
            <shortName evidence="1">DXP reductoisomerase</shortName>
            <ecNumber evidence="1">1.1.1.267</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">1-deoxyxylulose-5-phosphate reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-C-methyl-D-erythritol 4-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dxr</name>
        <name type="ordered locus">Ecaj_0473</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13717"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.267"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DXR family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.267" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304588.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRZ5"/>
    <dbReference type="SMR" id="Q3YRZ5"/>
    <dbReference type="STRING" id="269484.Ecaj_0473"/>
    <dbReference type="EnsemblBacteria" id="AAZ68510">
        <property type="protein sequence ID" value="AAZ68510"/>
        <property type="gene ID" value="Ecaj_0473"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0473"/>
    <dbReference type="eggNOG" id="COG0743">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035714_4_0_5"/>
    <dbReference type="OMA" id="PIDSEHF"/>
    <dbReference type="OrthoDB" id="1200722at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00092"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030604">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00183">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003821">
        <property type="entry name" value="DXP_reductoisomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013644">
        <property type="entry name" value="DXP_reductoisomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013512">
        <property type="entry name" value="DXP_reductoisomerase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026877">
        <property type="entry name" value="DXPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036169">
        <property type="entry name" value="DXPR_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30525">
        <property type="entry name" value="PTHR30525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08436">
        <property type="entry name" value="DXP_redisom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02670">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13288">
        <property type="entry name" value="DXPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006205">
        <property type="entry name" value="Dxp_reductismrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69055">
        <property type="entry name" value="SSF69055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00243">
        <property type="entry name" value="Dxr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000020258" description="1-deoxy-D-xylulose 5-phosphate reductoisomerase">
        <location>
            <begin position="1"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00183"/>
        </source>
    </evidence>
    <sequence length="387" mass="42563" checksum="421D2274DFA08F79" modified="2005-09-27" version="1">MKTVSVFGSTGAIGQMIIDIIFSELDKYQVKVLVAKSNIQLLAFQAKLVNAERVVITDVNLYRELKDLLLDTNIKVSAGDDGMIMATSLDVDYAMMAIVGIAALVPMTYLINSNVKVIALANKESIVCGGALLLSLAKEKDVKIIPLDSEHNAIYQILADKGHKDLEKITLTASGGPLLSMDYEQMKYVTVQDTVKHPVWKMGKKISVDSATMINKSLEIIEAYYLFSIKAEKLDVIIHNESVVHGIISYIDGTSIAFMSVPDMKIPIMYSLSWPNRSAALCKKLNLALYNQLTFMKPDIYKFPGIKLGFEVLKTSNVHANGIILNAANEIAVNAFLAKKIGFLDIVNIVYETLNLVNYGRINSLSNILDCDAISRKVASSTIDKLC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRZ7</accession>
    <name>ISPG_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)</fullName>
            <ecNumber evidence="1">1.17.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispG</name>
        <name type="ordered locus">Ecaj_0471</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:43604"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:10623"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IspG family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304586.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRZ7"/>
    <dbReference type="SMR" id="Q3YRZ7"/>
    <dbReference type="STRING" id="269484.Ecaj_0471"/>
    <dbReference type="PRIDE" id="Q3YRZ7"/>
    <dbReference type="EnsemblBacteria" id="AAZ68508">
        <property type="protein sequence ID" value="AAZ68508"/>
        <property type="gene ID" value="Ecaj_0471"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0471"/>
    <dbReference type="eggNOG" id="COG0821">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042258_1_0_5"/>
    <dbReference type="OMA" id="PTCGRTQ"/>
    <dbReference type="OrthoDB" id="665306at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00096"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046429">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00159">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016425">
        <property type="entry name" value="IspG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004588">
        <property type="entry name" value="IspG_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30454">
        <property type="entry name" value="PTHR30454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04551">
        <property type="entry name" value="GcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004640">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00612">
        <property type="entry name" value="ispG_gcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000011464" description="4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)">
        <location>
            <begin position="1"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00159"/>
        </source>
    </evidence>
    <sequence length="422" mass="46098" checksum="090ADCF714CFE899" modified="2005-09-27" version="1">MECDVINRILNEEDLFDCIKRKSKLTYEVNVGNVIIGGNNPIVVQSMALGGSGDANKDAHEVLELAKAGSELVRVAVNSEQAIKNIPYIRDVLVDNGFDHKMIIGCGQYEIARLVKEYPECASALGKIRINPGNIGFGNKRDKNFEDVIEFAIKYDIPIRIGVNWGSLDKYLASKLMNDNALLSNPKPDYIVLQKALVISAVTSAKRAEEIGLSKNKIVISCKTSKIQDLIPVYTVLSNVCNYPLHLGLTEAGSGIKGVVGSVAGISYLLLNGIGDTIRVSLTQQPGESRTTEVKLCQEILQSIGLKNFNAQVTSCPGCNRTNPKYFHQLVKDVNDYIADRMPVWRNTNPGAKDMVVAVMGCIVNGPGESKHANLGISLPGYGERPVAAVYQDGEKLCTLEGKNIFEQFVSIIENYVSVNYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS15</accession>
    <name>Q3YS15_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304568.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68490">
        <property type="protein sequence ID" value="AAZ68490"/>
        <property type="gene ID" value="Ecaj_0450"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0450"/>
    <dbReference type="HOGENOM" id="CLU_841288_0_0_5"/>
    <dbReference type="OrthoDB" id="2107852at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="330" mass="38284" checksum="290B353915B0FA41" modified="2005-09-27" version="1">MMLINNSSTSVLTRSFLFKKIKESYHDIDNILANTLSIIDIKDYCICITESFENHFILLVKGENIKVVDNNGFCAAALLPTKDMYKFLRCNPPCITTSYRRKMNLVIHNCTINSINNDEIYLSLIDFQNNAYFYDKQGKKLYLMDENKVQCSIDDYIIFSENNNPYRKKTFCYNNLGNLCFSDGYVVLSLLDNKYNTYYYNNNGLVLHDNMHNIYSDNESLYYDTCDISFMTWSELLCKVMKSITFKHYSTNSIKDYFAAHDNITFDLFDSAVIKDDLSFDMSCTCEIKSFSQSLDGIYINVENATNPNSVLKNYTIVHENQSSNTLYNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS24</accession>
    <name>Q3YS24_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Class I peptide chain release factor domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0440</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0440"/>
    <dbReference type="EnsemblBacteria" id="AAZ68481">
        <property type="protein sequence ID" value="AAZ68481"/>
        <property type="gene ID" value="Ecaj_0440"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0440"/>
    <dbReference type="eggNOG" id="COG0216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_5_1_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="4">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="173" mass="20102" checksum="0E509E7946317D0D" modified="2005-09-27" version="1">MKFESGVHRVQRVPETEASGRLYTSAATVAVLPEIEEVDLKIDEKDLRIDVYRSSGPGGQSVNTTDSAVRITHIPSGIVVIQRDEKSQHKNKALKVLRARLYNLEKQKRDSEISQMRKSQIGSGDRSERIRTYNFPQSRITDHRINLTLYRLEDIMKKWQSIYIFYTKDFKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS45</accession>
    <name>Q3YS45_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">DNA gyrase subunit B</fullName>
            <ecNumber evidence="4 12">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">gyrB</name>
        <name evidence="14" type="ordered locus">Ecaj_0417</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="12">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type II topoisomerase GyrB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="4 12"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304538.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0417"/>
    <dbReference type="EnsemblBacteria" id="AAZ68460">
        <property type="protein sequence ID" value="AAZ68460"/>
        <property type="gene ID" value="Ecaj_0417"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0417"/>
    <dbReference type="eggNOG" id="COG0187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006146_4_1_5"/>
    <dbReference type="OMA" id="LWETTMH"/>
    <dbReference type="OrthoDB" id="205481at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03366">
        <property type="entry name" value="TOPRIM_TopoIIA_GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01898">
        <property type="entry name" value="GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002288">
        <property type="entry name" value="DNA_gyrase_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011557">
        <property type="entry name" value="GyrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034160">
        <property type="entry name" value="TOPRIM_GyrB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00986">
        <property type="entry name" value="DNA_gyraseB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01059">
        <property type="entry name" value="gyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="13">
        <location>
            <begin position="430"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAZ68460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="798" mass="89534" checksum="8567768C8C172FE1" modified="2005-09-27" version="1">MDNYNASSITVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVIDNSIDEALAGFCNKIDIVIQSDGSVSVTDNGRGIPVDMHEDEGMSAAEVIMTQLHAGGKFDHSSYKVSGGLHGVGISVVNALSTWLELTIWRNGKEYFMRFEDGVTVKPLSVVGENTYKKGTRITFLPSKNIFSSVNFNYSTLESRVRELSFLNSSININLKDLRGAQVLNSSFGFRDNSKVSDASDKGTSGFVKYLDRNKTAVTKIVHIEGIVLEHGISLELSMHWNDSYYENMLCFTNNIRQRDGGTHLAGFRSAMTRCINNYATNEGFLKKAKVSILGEDVREGLTCVLSIKLPDPKFSSQTKDKLVSSEARTVVENIVYEKLTNIFETNPKLAASIIERIIRSAKGREAARKARELIKSKNGFDFTILPGKLADCQEKSPELSELFIVEGNSAGGSAKQGRDRRSQAVLSLKGKILNVERASLDRILSSAEIASLITAIGSNIGTENFDIEKIRYHKVIIMTDADVDGSHIRTLLLTFFFRYMREVIERGYLYIAQPPLYKVTKHKKEFYIKNDDMLHDYIISNAIKDLTLTGTNTYSGEDLSQILHQCMNISKITKSYDRIIPQSVLESLIIFFKKHSFDSADAISEHLNNVFCEYEWSVEIPEKDKISIFRLSHGLVDKYYIDNTIMNSDEIRQVIHICDGIDEIFNSMLILSTKDNNIKINSPTSLVDNIIEFGKKDMTLQRFKGLGEMNSDQLWDTTLNPETRTLLKLKISDCEAADSIFSVLMGDFVEPRRDFINNNALNVQNIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-08-12" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS48</accession>
    <name>Q3YS48_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0414"/>
    <dbReference type="EnsemblBacteria" id="AAZ68457">
        <property type="protein sequence ID" value="AAZ68457"/>
        <property type="gene ID" value="Ecaj_0414"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0414"/>
    <dbReference type="eggNOG" id="COG2913">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149220_0_0_5"/>
    <dbReference type="OMA" id="WRRIESM"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="145" mass="16691" checksum="79ED5A7F4DA007E2" modified="2005-09-27" version="1">MVAYSIFVFENVMLARVICIALFMLTGCYTTTIHHGYPTSEINLWDGVKIGDLNTQVVQLIGDPCIIEGNVWYYISYSIYKKRFFSAKEYESIVLKLTFDPQTNEVIEVKNIKVDKRNLVKLLKKDTSVTGIHDSLLRKFISKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS58</accession>
    <name>Q3YS58_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304525.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0404"/>
    <dbReference type="EnsemblBacteria" id="AAZ68447">
        <property type="protein sequence ID" value="AAZ68447"/>
        <property type="gene ID" value="Ecaj_0404"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0404"/>
    <dbReference type="HOGENOM" id="CLU_782420_0_0_5"/>
    <dbReference type="OMA" id="AYSGFWY"/>
    <dbReference type="OrthoDB" id="1091247at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="357" mass="40271" checksum="5274061DFDF0DD05" modified="2005-09-27" version="1">MKYFNKKLIVIPSVVITLLSSLYVGTWLVIATHVKATISKALIHIDASYNDNIQITGFPFTPKIKILNLKINLPSLNIYAPSVSLKYRLLSDTLEFSGENTTLKSENSTKIINNEQQYTLNCQLNDKFNLLVKPSKNLILSLLDNKNDKKAYFSQIIYEDNGISCNNNNITNKNSISIKTNEHTSPDNLLTMLQYKINANIIGNSDTNTNIKIKDAIMTLTTSDTEFKEISAVIDNIGITFKKSLISIYGTLLFPLSMDYNKTNNEGLTIEISGIKDIIRQLVETISHKSNNADKISYILQNYIYSIAKKKENGNILLSISQTTNPFNIFICKIPYEEFINKIKLITNTTNKSANKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS66</accession>
    <name>Q3YS66_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RDD protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0396</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304517.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0396"/>
    <dbReference type="EnsemblBacteria" id="AAZ68439">
        <property type="protein sequence ID" value="AAZ68439"/>
        <property type="gene ID" value="Ecaj_0396"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0396"/>
    <dbReference type="eggNOG" id="COG1714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053152_3_0_5"/>
    <dbReference type="OMA" id="IVQFIML"/>
    <dbReference type="OrthoDB" id="1974702at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="6">
        <location>
            <begin position="28"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="196" mass="22959" checksum="C197C8E9050AD1E7" modified="2005-09-27" version="1">MFLYNYLPFLKKNKQPQDENTICYSGGLRRLTSILIDFAILIFILQIIQNVYSYFFMYEQTDNTVIKIIEKYKMSVPLTEQEIQIKNKYLYKAICGQIIQMIAMYIYAVYSWVKFSATLGKFLIGLRVVDATTFQKITLTQSTKRVFGSILSCIPLCLGILWCNFNKRKQTWHDKIANTVVVTNKSLKNYHNNHNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS91</accession>
    <name>Q3YS91_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exodeoxyribonuclease III</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0371</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304492.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0371"/>
    <dbReference type="EnsemblBacteria" id="AAZ68414">
        <property type="protein sequence ID" value="AAZ68414"/>
        <property type="gene ID" value="Ecaj_0371"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0371"/>
    <dbReference type="eggNOG" id="COG0708">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027539_0_1_5"/>
    <dbReference type="OMA" id="WWSYRGR"/>
    <dbReference type="OrthoDB" id="1893707at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008853">
        <property type="term" value="F:exodeoxyribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09086">
        <property type="entry name" value="ExoIII-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037493">
        <property type="entry name" value="ExoIII-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43250">
        <property type="entry name" value="PTHR43250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="279" mass="32457" checksum="89307E44DF30C6B1" modified="2005-09-27" version="1">MTLKIATWNVNSIRKRLDHLRHWLVKYDIDIALLQEIKCTNDQFPFLDLESLGYKCYVNGQKARNGVAIITKYPIVEELVTDIFSSDYKSSLCYKLSSEGFIYNYQESRYLECVVLYNEVKVRVASIYIPNGQSVDSDAFQYKLGFFDQLIDRALFLLNKEEILILGGDYNVAPYPIDVYSTEIMDGRLCFHQYEREKFRVILNLGFTDAFRVLNEHDQKFSWWNYKAGAWQENRGLRIDNLLLSPQAVDKLLSCVIHDELRGLDTPSDHAPVICTLEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSA9</accession>
    <name>Q3YSA9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydrofolate synthase/folylpolyglutamate synthase</fullName>
            <ecNumber evidence="6">6.3.2.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Folylpolyglutamate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">Ecaj_0353</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Functions in two distinct reactions of the de novo folate biosynthetic pathway. Catalyzes the addition of a glutamate residue to dihydropteroate (7,8-dihydropteroate or H2Pte) to form dihydrofolate (7,8-dihydrofolate monoglutamate or H2Pte-Glu). Also catalyzes successive additions of L-glutamate to tetrahydrofolate or 10-formyltetrahydrofolate or 5,10-methylenetetrahydrofolate, leading to folylpolyglutamate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n) + ATP + L-glutamate = (6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:51912"/>
            <dbReference type="Rhea" id="RHEA-COMP:13257"/>
            <dbReference type="Rhea" id="RHEA-COMP:13258"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:136572"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>10-formyltetrahydrofolyl-(gamma-L-Glu)(n) + ATP + L-glutamate = 10-formyltetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:51904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13088"/>
            <dbReference type="Rhea" id="RHEA-COMP:14300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:134413"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7,8-dihydropteroate + ATP + L-glutamate = 7,8-dihydrofolate + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.12" evidence="6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304474.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0353"/>
    <dbReference type="EnsemblBacteria" id="AAZ68396">
        <property type="protein sequence ID" value="AAZ68396"/>
        <property type="gene ID" value="Ecaj_0353"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0353"/>
    <dbReference type="eggNOG" id="COG0285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015869_1_1_5"/>
    <dbReference type="OMA" id="YFEMGTL"/>
    <dbReference type="OrthoDB" id="1232874at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00157"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="PTHR11136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="12" id="KW-0133">Cell shape</keyword>
    <keyword evidence="15" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="11" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="18 20" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="19">
        <location>
            <begin position="47"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00000809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AAZ68396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="434" mass="47887" checksum="392EC8B9F007EB60" modified="2005-09-27" version="1">MVYMPHWPKPLGLAPADLVCDMIPGRMKRLLDNLGNPEKKLPPVVHVGGTNGKGSTMSFIRYILNSAGLKTHVYTSPHLIEFNERVVIAGNRISDKYLYEVLEVCREASADIPVTFFEGTTAAILLAFSEIKADIVLLETGVGGRLDATNVVSPILNIITSISLEHTEVLGDTVELIAGEKAGIMKENVTCVIAPQDKESIMNVLEYYAIKKNVPLYRGGIDWFCERKEDKMIFKNNIEELLFPLPSLRGEHQIMNAGNAIAACSILSGKFGYNISYENIVQGLTQAYWPARLEKLTTGFLAKMVPQAWELFLDGAHNPAGAEVLSRWIRNNELGDLYIIIGMTREKDSVEFLNYLKPYVKFLASVCVKSEPRAQTAEELLKTALSLGIKAVASRTLSDAISKILEVGDHNKKSTILICGSLYLAGDVLLENNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSB8</accession>
    <name>Q3YSB8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304465.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68387">
        <property type="protein sequence ID" value="AAZ68387"/>
        <property type="gene ID" value="Ecaj_0344"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0344"/>
    <dbReference type="HOGENOM" id="CLU_399412_0_0_5"/>
    <dbReference type="OMA" id="IVCCARV"/>
    <dbReference type="OrthoDB" id="825138at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="759" mass="87278" checksum="50E99A456878E4BC" modified="2005-09-27" version="1">MKSSIFNKHVTFGILSVGVVCFCAVLINEYSKNSISFVTFAALMSLCLALLLASALLCYGLGSRLVDDSKRAIDFLPCKSGSFIPYRLEPRFHDVSSMSLHVYDDLCNISQDLKLLVSQPSKMTHNDFYNPASDRCCLLFLNFRRCFDSMLDKLECGNVFIKEDHINIVDVERLLTQEDRTFVILAAQLIVRNRVLYSTYPEAVYEKILKLANLSVKEVPFQERLNAGNRIIKLLEDKVYSFSNEGCFHKFLENIFHEYMSFKDAHVINSPSDFYKMRDHDMKLKLLKDVFCASYFKYYSIYQDKQHDMVRRGVDFASCIIQHYDESATRYDSDIVQYLNEMRKSGFVVDAGISNASIILNAREIISKVFEQTAVMDNGYVSCFSRYSFEKLSTVKNSAMKMVQGLASSEFGNNIIEKLDACALVYHMTLSTSPYNRMFISQRLTTSVNKEELIFNIYHSLYHYANVVKKVSNSIDNFRAYEAVSTNIQDEEYYILYPLLKSVLDTGLSILHTKIKPGSPISQDDVYKIFSSYDVSKRILSHNRRKNIFEEIVCCARVTCCLPHLLKTYPDDVVNHIKALANFDIGENKYHCVEYLSHCYDICVFSNVDEFQQMMNSFLASYSSYYQSLDERLKRKYTSFQSGSSFDIVLMHLRYTSLYNSEMVEQHLIDDACDRMKSLDFDRLRNFYMSFMQVIANTMNFGHDQIYFPLYEVNTLQNDVENDVVSDKDTVCESMLCHSLPSTSVGGTCGLDSGSAIAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSC0</accession>
    <name>Q3YSC0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0342</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304463.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68385">
        <property type="protein sequence ID" value="AAZ68385"/>
        <property type="gene ID" value="Ecaj_0342"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0342"/>
    <dbReference type="HOGENOM" id="CLU_1270646_0_0_5"/>
    <dbReference type="OMA" id="EMPRDEQ"/>
    <dbReference type="OrthoDB" id="2118317at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="217" mass="24355" checksum="1298E98A8BB3119C" modified="2005-09-27" version="1">MIKTHDIAINTISAIMVTAAATGLALCSIGIIREHQYEYCALCVLLLLSACVSLIANYKKGISRKCASIRNYLSKISSDESLDGENKYYDPAPDLFAEHYFDEDAPSADRAEKIHVDRRQYYSEPYENEEQGQYSAYEEALHHSGWIRSQPFFAEYDTTPPDNRSAEMKTFSSSSSEAKVDTTIRGMQDKNNSKIQITDSRVEAVKPLSEEQGKGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSC8</accession>
    <name>Q3YSC8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Parvulin-like PPIase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Peptidyl-prolyl cis-trans isomerase plp</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Rotamase plp</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0334</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PpiC/parvulin rotamase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304455.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0334"/>
    <dbReference type="EnsemblBacteria" id="AAZ68377">
        <property type="protein sequence ID" value="AAZ68377"/>
        <property type="gene ID" value="Ecaj_0334"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0334"/>
    <dbReference type="eggNOG" id="COG0760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023843_2_1_5"/>
    <dbReference type="OMA" id="DNSQGWI"/>
    <dbReference type="OrthoDB" id="1201303at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13145">
        <property type="entry name" value="Rotamase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PpiC" evidence="9">
        <location>
            <begin position="229"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="630" mass="71558" checksum="577384F42159B819" modified="2005-09-27" version="1">MKSKGLFVKPLLIILVCSLVFIAFGTSFFPGNFNNDKYVAKIGHEKLSLQDYTNAYHDELRYIQQILNRPLTEEQIAQFNIKLSVLNKLIENKVLTKFTDSLNLKVGEKSILSHIKSIKFFQDENGNFDKTKFNIGLSNAGLTERLYINKLEKAFPVAMLMSCLFSGTQNTYVKYHPELIKQILQNLHQARTIDLIEISPSTNDISVPSLDELKKLYEEKRKSGNLTFPENRVIEYLTINYKNFINQINVTQEDIDNEIKTEELDEQRDILNLVFSTKNEAEAALKALNEGTDFNDVVTNIAHTTTDNITLNNIVKNTLPNDIRGKVFSLKEGDISPILHSMFGWHIIKIKSIHKISTEDLQELSEKIAQNIKKKKAISLLTTEIESINDKINNGASINEIAKLYNLSIKTINTDISGKDLSGNKIKMSESNADAIILAAFSAQLNKPSNFTDNDDYFFSVNVTKVEPSREKTFDESKEQLVYEWQNDLKSQKLSKLTQEVVVKLKSGIDIHNIDGVLLNSNQMVYRNSVAAVDNPGQNYPTDLVDEIFNLKKGEISKSYQSSTNSSEQKMLIAILKDIKNADQVSNVDFKNIQEKIANDNLESLKNQLITYLTKKYSVKINQNLIDNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSD3</accession>
    <name>Q3YSD3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GATase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304450.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68372">
        <property type="protein sequence ID" value="AAZ68372"/>
        <property type="gene ID" value="Ecaj_0328"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0328"/>
    <dbReference type="eggNOG" id="COG2071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_865285_0_0_5"/>
    <dbReference type="OMA" id="INDFLYR"/>
    <dbReference type="OrthoDB" id="1503348at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase" evidence="1">
        <location>
            <begin position="44"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="321" mass="35936" checksum="163D0A66475AA320" modified="2005-09-27" version="1">MRYFVLILLFLFSTSAIGLEVASNKMIVGLVRTAKETYPEEWTFSNNIAEMLEKLGAEVILFDYNTIVDFKKIKNESLEEAKDDISLLAKIESDKIQDAVHNFIKENKINRIFIPGNYYNVDTEPYPPTPNRQLVTNAIVKIVESNPKIHLIGVCGGLQGIMHAVGVEVIRVKKIVTSKEAVEAHSISMPDPHRKNVGLHRLRVAPGSRLSSIVSKYVKPDDNGWIAIYFPDAHGGVVNNSQENIKKIESLGYKIVGFSDDGIIEAIEDKYGNILFQDHPEALAINAIKGSEQDVKDDNDVKRRQATLSAIAIINDFLYRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSF0</accession>
    <name>Q3YSF0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304433.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0311"/>
    <dbReference type="EnsemblBacteria" id="AAZ68355">
        <property type="protein sequence ID" value="AAZ68355"/>
        <property type="gene ID" value="Ecaj_0311"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0311"/>
    <dbReference type="eggNOG" id="ENOG5032Y4S">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175587_0_0_5"/>
    <dbReference type="OMA" id="HPIHHQD"/>
    <dbReference type="OrthoDB" id="1773874at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021277">
        <property type="entry name" value="DUF2610"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11020">
        <property type="entry name" value="DUF2610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="89" mass="9859" checksum="0B732DCBDF978A45" modified="2005-09-27" version="1">MPGIKKFIVPCDFNGQSSPFAIYIGEPKPENHPVQHQDNWLSKERGGTIPEKIKDSLSKLYALAQKNGICFADLCVYALTVAAHKNSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSF4</accession>
    <name>Q3YSF4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exopolysaccharide synthesis, ExoD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304429.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68351">
        <property type="protein sequence ID" value="AAZ68351"/>
        <property type="gene ID" value="Ecaj_0307"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0307"/>
    <dbReference type="eggNOG" id="COG3932">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093444_0_2_5"/>
    <dbReference type="OMA" id="FGLKPWL"/>
    <dbReference type="OrthoDB" id="2077544at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010331">
        <property type="entry name" value="ExoD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41795">
        <property type="entry name" value="PTHR41795"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06055">
        <property type="entry name" value="ExoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033239">
        <property type="entry name" value="ExoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="209" mass="23029" checksum="01D21AFF0EC9B07C" modified="2005-09-27" version="1">MSQEKNKAVSDLLEEVTNNTDVDRITLFELKSALHARGFGILMLLFSLPLSIPLPVPPGYTTIFSIPLLMFAFQMLLGFQVPWMPSWLEKKSFKRTTLALIIEKTAPILRKAEKLTKPRLLFIFNSVGEKVIAFISLLCAVSIAIPLPLTNFIPAGGVSIMSLGLLNKDGMMVILGVLLSLFGIVVTTMVIILGPKLVVSMFSFLSKFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSG1</accession>
    <name>Q3YSG1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyr_redox_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304422.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0297"/>
    <dbReference type="EnsemblBacteria" id="AAZ68344">
        <property type="protein sequence ID" value="AAZ68344"/>
        <property type="gene ID" value="Ecaj_0297"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0297"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0493">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0543">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006653_0_0_5"/>
    <dbReference type="OMA" id="SCIYQKQ"/>
    <dbReference type="OrthoDB" id="14392at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="362"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1130" mass="128310" checksum="3F6C9E06AE4B9E9D" modified="2005-09-27" version="1">MLNFELTVQNLYTREGLVKLDNLFLCYLQSHNQNLYDTLIRERNNESKSNNTSCIIIELSYIVEDFISKLFSIENEIIIQQNTHKEFSEIYKCKRLFIQRYALKKYPDINSLNISEITEQIRELFLFPIEDKKFAKQTLLWLEDKENHSEKLDITARYAAYMIHSGSHSILFSVPNKTDVYNLVPTITKTVNNDTIAMTSKRIKRRDGFNLTHKQPTLYNALNNSHYCIFCHKQNKDSCSKGLLENNNEFKKSILSTELHGCPLEQKISEMNLVKSQGYPIAALAIIMIDNPLCILTGYHICNDCTKSCIYQKQDPVDIPMVETQIIDNVLSLPYGFEIYSLLTRWNPINFERPLPKTNTDKNVLVIGLGPAGINLSHHLLNDGHTVIAIEGLKIEPLPENISGVTQSGEKTEFNLIKNVETELYDNLSERTPYGFGGVSEYGITARWNKNYLKIARLILERRKNFSMYGGVRFGSTLTIENAFEIGFHHIALATGSGSPNIINIPNSLARGVRMASDFLMSLQLTGAARATSIANLQIRMPIIVIGGGLTAIDAATEALAYYPLQVEKFLTRHEALIENYGKSYIEQNWTEEEKIIANEFLNHAMQIRKEKTLAKIENRQAKIIELLQSWGGVTIVYRNKLLHAPSYKLNSYEINNALAEGIYFIENLQPYEMITDQYNHISNIKFTDQYNNKKTLNAKTIIIAVGTKPNLTSIKENQQFCGLDQDFTHTFDLQGISTKITTSPKFTKKDSIFISPDKKISIFGDLHLPYRGSVVKAMASAKNGYPIITKVLNQCPPSKYTNLLTTLNHKLKAYVIDIENITKTITKLTILAPLAAANFKPGQFYRLQNFEYNSLNTENTQLSMESIALTGVSVDKSKGLISTIVLNTGGSSHLCNYLKKNEPIIFMGPTGTPTEIPHNQNIMLIGGGVGNAVLFSIGKALLSNNCKVLYFAGYKKVEDVFERSSIEESSSTVVWCCDETKIEPRRTQDKSYQGNIIDAILQYNNNLSETSDIPLHSIDRIIIIGSSYMMDAVSYSIFNQLQTLFKKEVKVIASINSLMQCMMKEICAQCLQKHIDPITKQESFVYSCNNQDQYANYVDFKFLNERLKQNTLQEKCTKQWIKHCLHKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSG3</accession>
    <name>Q3YSG3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CDP-alcohol phosphatidyl transferase</fullName>
            <ecNumber evidence="4">2.7.8.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0295</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8" evidence="4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304420.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0295"/>
    <dbReference type="EnsemblBacteria" id="AAZ68342">
        <property type="protein sequence ID" value="AAZ68342"/>
        <property type="gene ID" value="Ecaj_0295"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0295"/>
    <dbReference type="eggNOG" id="COG1183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049944_1_0_5"/>
    <dbReference type="OMA" id="IFFDFFD"/>
    <dbReference type="OrthoDB" id="1232102at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012616">
        <property type="entry name" value="CDP-OH_P_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08009">
        <property type="entry name" value="CDP-OH_P_tran_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="CDP-OH_P_tran_2" evidence="3">
        <location>
            <begin position="205"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="259" mass="29636" checksum="569DC76B714E6068" modified="2005-09-27" version="1">MKEEQSSKFFPIARLFPNIVTLLGLCAGLTSIKFTFIGKWESAVMFIIIAALIDGMDGRIARLLNSTSGFGAHLDSFADFLNFGAAPAFLIYFWSLKQIKVIGWMLVMMFVMCMSIRLARFNVSLYEEKKLDWHKFFFVGVPAPLGAVLSLIPIMLTFYYHDNHWLVENLSDQNNVAIYFAIIAFLLVSNIPTFSAKHLYIPKNLSYIFILLFGIFIVFAITKPWLTFPVFGIMYILSIPISSSVYIYIMYKNKTKINM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSH8</accession>
    <name>RPOZ_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit omega</fullName>
            <shortName evidence="1">RNAP omega subunit</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase omega subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit omega</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoZ</name>
        <name type="ordered locus">Ecaj_0280</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase subunit omega family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304405.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSH8"/>
    <dbReference type="STRING" id="269484.Ecaj_0280"/>
    <dbReference type="EnsemblBacteria" id="AAZ68327">
        <property type="protein sequence ID" value="AAZ68327"/>
        <property type="gene ID" value="Ecaj_0280"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0280"/>
    <dbReference type="eggNOG" id="COG1758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125406_2_1_5"/>
    <dbReference type="OMA" id="EECMGRT"/>
    <dbReference type="OrthoDB" id="1730229at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00366">
        <property type="entry name" value="RNApol_bact_RpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003716">
        <property type="entry name" value="DNA-dir_RNA_pol_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34476">
        <property type="entry name" value="PTHR34476"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="SSF63562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00690">
        <property type="entry name" value="rpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000237454" description="DNA-directed RNA polymerase subunit omega">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="138" mass="15431" checksum="960968D380F57BA2" modified="2005-09-27" version="1">MARLTVEECMGRTNNKFKLVILASQRAHDLNSGACPVIKHKNGKNTVIALKEIAAKQLDVSSLFNLSVQRCRKYMEKFINSDEQCVTNKSKIDIFQQNAITSSFNELGNNSNNQGSNLLGRDNFFSTPENRNTSNTDS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSI0</accession>
    <name>THIC_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="1">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylpyrimidine phosphate synthase</fullName>
            <shortName evidence="1">HMP-P synthase</shortName>
            <shortName evidence="1">HMP-phosphate synthase</shortName>
            <shortName evidence="1">HMPP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiC</name>
        <name type="ordered locus">Ecaj_0278</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSI0"/>
    <dbReference type="SMR" id="Q3YSI0"/>
    <dbReference type="STRING" id="269484.Ecaj_0278"/>
    <dbReference type="EnsemblBacteria" id="AAZ68325">
        <property type="protein sequence ID" value="AAZ68325"/>
        <property type="gene ID" value="Ecaj_0278"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0278"/>
    <dbReference type="eggNOG" id="COG0422">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013181_2_1_5"/>
    <dbReference type="OMA" id="TWELFRD"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025747">
        <property type="entry name" value="ThiC-associated_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="PTHR30557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13667">
        <property type="entry name" value="ThiC-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000242258" description="Phosphomethylpyrimidine synthase">
        <location>
            <begin position="1"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="315"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="356"/>
            <end position="359"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="543"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="546"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="551"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <sequence length="565" mass="63663" checksum="EA8F8C96D17DBA42" modified="2005-09-27" version="1">MILYNIFLVKMKVDFINTVFPSSTKEYIPGTIYNNIKVGMRKIHFNDNTESILVYDTGGLHTDQNVEVDINHGITKLRLNWIIDRQDVEYYERQPVLNSEHIFTSQSNKVLRANSTKPVTQIFYARNNIITPEMEYIAIRENSLRQKILSYKPTVMSPEITPEFVRQEVASGRAIIPANINHPESEPMIIGKNFLVKINANIGNSVVSSNIEEEVQKMIHAITYGADTVMDLSTGNNIHDIREWIIRNSPVPIGTVPIYQALNKVNGVVGDLNFNVFKETLIEQAEQGVDYFTIHAGVLKNYISHTDNRLTGIVSRGGAIMAHWCTIHNKENFLYTNFEEICDIMKRYDIAFSLGDGLRPGSIADANDTAQFLELKTLGELTDIAWQHDCQAMIEGPGHVPMHLIKENMEKQIHFCKEAPFYTLGPLTTDIAPGYDHITSAIGAAITGWYGTSMLCYVTPKEHLGLPNIQDVKDGVIAYKIAAHAADLAKGNPSAYIRDYALSYARFNFKWHDQFNLSLDPETARSLHDESLPSEHAKSAHFCSMCGPKFCSMKLTHHLKEHSTG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSI5</accession>
    <name>PDXJ_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxine 5'-phosphate synthase</fullName>
            <shortName evidence="1">PNP synthase</shortName>
            <ecNumber evidence="1">2.6.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxJ</name>
        <name type="ordered locus">Ecaj_0273</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = H(+) + 2 H2O + phosphate + pyridoxine 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:15265"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="EC" id="2.6.99.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.99.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304398.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSI5"/>
    <dbReference type="SMR" id="Q3YSI5"/>
    <dbReference type="STRING" id="269484.Ecaj_0273"/>
    <dbReference type="EnsemblBacteria" id="AAZ68320">
        <property type="protein sequence ID" value="AAZ68320"/>
        <property type="gene ID" value="Ecaj_0273"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0273"/>
    <dbReference type="eggNOG" id="COG0854">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074563_0_0_5"/>
    <dbReference type="OMA" id="SNAGWDT"/>
    <dbReference type="OrthoDB" id="1159993at2"/>
    <dbReference type="UniPathway" id="UPA00244">
        <property type="reaction ID" value="UER00313"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033856">
        <property type="term" value="F:pyridoxine 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00003">
        <property type="entry name" value="PNPsynthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00279">
        <property type="entry name" value="PdxJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004569">
        <property type="entry name" value="PyrdxlP_synth_PdxJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036130">
        <property type="entry name" value="Pyridoxine-5'_phos_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30456">
        <property type="entry name" value="PTHR30456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03740">
        <property type="entry name" value="PdxJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63892">
        <property type="entry name" value="SSF63892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00559">
        <property type="entry name" value="pdxJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000231804" description="Pyridoxine 5'-phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>3-amino-2-oxopropyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>3-amino-2-oxopropyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>3-amino-2-oxopropyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="211"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>3-amino-2-oxopropyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00279"/>
        </source>
    </evidence>
    <sequence length="238" mass="26339" checksum="CDBA892E8490705B" modified="2005-09-27" version="1">MSSIALGVNIDHIATLRNARNVDYPCIIEVANIAVNNGADFITVHLREDRRHIMDDDVFRLKSSLKVPLNLEIAATDEMLSIAIEVKPKCVCLVPEKRQELTTEGGLDVGSIFSYLVSFIEKLHSYDIDVTLFIEPDISQIDLAKKLHANNVELHTGKYCNNATQNELSKIIKAAEHCHHQNITCHAGHGLNYQSAATIAKVPYISALNIGHFLICEAVLHGIGKSIYKMKQVIASTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSJ1</accession>
    <name>Q3YSJ1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Malonyl-CoA decarboxylase</fullName>
            <ecNumber evidence="3">4.1.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0267</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304392.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0267"/>
    <dbReference type="EnsemblBacteria" id="AAZ68314">
        <property type="protein sequence ID" value="AAZ68314"/>
        <property type="gene ID" value="Ecaj_0267"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0267"/>
    <dbReference type="eggNOG" id="COG1593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023433_3_1_5"/>
    <dbReference type="OMA" id="PIDWSTP"/>
    <dbReference type="OrthoDB" id="864913at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050080">
        <property type="term" value="F:malonyl-CoA decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038917">
        <property type="entry name" value="Malonyl_CoA_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007956">
        <property type="entry name" value="Malonyl_CoA_deC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042303">
        <property type="entry name" value="Malonyl_CoA_deC_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035372">
        <property type="entry name" value="MCD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038351">
        <property type="entry name" value="MCD_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28641">
        <property type="entry name" value="PTHR28641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05292">
        <property type="entry name" value="MCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17408">
        <property type="entry name" value="MCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCD_N" evidence="2">
        <location>
            <begin position="94"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="MCD" evidence="1">
        <location>
            <begin position="184"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="458" mass="52611" checksum="A81649EF3CB1F572" modified="2005-09-27" version="1">MDNSLPVIRSKTINSVFKVMGEMADAIFSWVGSINKDLTRDQDISNLYEKMKECVNPKGGEIKARYNTISLGNLYLNLSDVGKIAFFKLLEGKFSADRIAIDEKIGDYIRETDENKKRKCEFELMTVLESPRFHILKQFISLPDGLKFIVDMRADVMRLKDKCQLFFSLEKDLRHILSTWFDVGLLDLHQITWDSPASLLEKLILYEAVHAISSWDDLRDRLDSDRRCFAFFHYKMSVEPLIFVEVALVDEMATSIQMLLDSHVPPKDPKNAKVAIFYSISNTQRGLSGISLGNFLIKRVVNKLSEEFQHIKIYATLSPIPGFVRWLTKTLPDNLELLNDLKVNISIDDIISYVNNKQYADLTQDIKSLFLKLCAYYLVKAKSVDRALDPVAHFHLSNGAIIKQLNWMADISEKGLSSSMGIMVNYHYELSKIDDNYENYVINKEINCSKEVLSLLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSJ4</accession>
    <name>Q3YSJ4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0264</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304389.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0264"/>
    <dbReference type="EnsemblBacteria" id="AAZ68311">
        <property type="protein sequence ID" value="AAZ68311"/>
        <property type="gene ID" value="Ecaj_0264"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0264"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_0_5"/>
    <dbReference type="OMA" id="MAVICPL"/>
    <dbReference type="OrthoDB" id="961781at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="9"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="415" mass="45816" checksum="3EA1BC54E8768479" modified="2005-09-27" version="1">MSHSLNKKAIFSLLLCAFIECYDFLVYGNFGRIFSQIFFSQINNESLSLILSFAIFAIAFFVRPIGSLLFGYIGDKYGRKVSLFVSASMLILSVGGIAFLPTVDSIGVLAPILLVLLRTLQGLSFGGEVGAIVLIAESVSKNKVPALLSLHFALAIIGGATGSFIFKLCYNFIPEVQFYSWGWRIPFIIGLIMSIILPFLRHSIEESRQYLDYTSGKKVSKVPILDIVLRYKKICIITFSLIGAANALFYMFFVFLDVQQQLSIMGYCLLILTMLASGFISFLLFQIYKPENVVFIIQVVFITCVSPAVCFLGLNSVISYFILAVILGLYATPLLSMLVFLLPVSIRQTGFSMCYSIAVAVFGGATPAVCLWLSEITQSSISPIIYLDCCALLSLVGILCLKQHNAKKCEQVMYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSK5</accession>
    <name>Q3YSK5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0253</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304378.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0253"/>
    <dbReference type="EnsemblBacteria" id="AAZ68300">
        <property type="protein sequence ID" value="AAZ68300"/>
        <property type="gene ID" value="Ecaj_0253"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0253"/>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007148_0_0_5"/>
    <dbReference type="OMA" id="IAGNIIW"/>
    <dbReference type="OrthoDB" id="646292at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025195">
        <property type="entry name" value="GTA_TIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032876">
        <property type="entry name" value="J_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13547">
        <property type="entry name" value="GTA_TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13550">
        <property type="entry name" value="Phage-tail_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTA_TIM" evidence="1">
        <location>
            <begin position="394"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="Phage-tail_3" evidence="2">
        <location>
            <begin position="706"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1206" mass="139672" checksum="CAE76E48CEDC5E49" modified="2005-09-27" version="1">MSTTILSTIINTIKESIAKLLSSELSNITNQDIQTHILGNNTQTKHIPKINHLKIQTSTYGKMIPMLYGSMRIAGNIIWMDKIKITTDEDIVIINNPNKKPTTHSSTQYKYSTSLAIAICKGYVKKISKIWANSQLLDLNTLKYRFYQGTEDQEPDSLMLKLMGDTPAYRGLSYIVIENLPLTEYKIPNFTFEVTAYPFNYTYQDHITHKIKSIHIIPGSGEFAYDTKIQMKVFQQKIGNQYIPYGEAQKINNHSQQKKADALISLDQLQETLPNVQWISVIVNWYTDSLNIKDCNIYPAVEFKFDATTIPDNWNVAGINRESARKISNYNNSTLYSSCGTTNDKSLIRYINELKTRGYKVLLYPMLLADTQDNKWIGNLTGDVEHITTFFNKQYNPFIKHYCNLTKDIIDAFIIGSGFIGLTSIKNNHNHYPAVVELTKLANYVKNQVGNNTIVTYAADWREYHSHNGYYNMDTLWSSKDIDVIGINAYFPLTDTPQPTNGFTEQDIINGWHSGEGYDYFYTDPEKKQGKIPYIENNNQYSWKDIEKWWNRYHINPDNTITSWIPKSKKIWFTQYGFQSIDNCTSQPSISIDSQYNKKPIFSKGHADFRAQKIAISGTIKTWNNSNIVEQMFLWGWDVRPYPYFPQLQNIWKDAKNWQIGLWIQEKLSLLEISNIIYDLLKNTGLENNQINIQNLHNEIDGYIITETQSAKSILNSLKDLYNFEIVEQDNKLSIIQENQPTQYNIPSDDILLNNKNNTQKLTKTKPFNLVNSINFMYINKNNEYNISSQRVKTHNLQNTITQTIQVPVVLSNDQAKTIAENILNKKSNNKYSFYLALPIKYVWLDTNDVIQVEYNNFSYKIKIINIYINNLSINIEGIIYNHTLTNQSFITNNDFNDNPEKHTIETPVQILDLPCINNIDNVLYFAITRIKENWEGSILYSYGNQNEHYKDVLPIDTEATTGITLHNLDIGPIAIPDEANKITIFLKTGQLHSIKSLFANNNSNLALIGNEVIQFQNVIQIRQNTYQLNYMLRGRFGTEKYIDKHKKEEFFILLDNLPYLKINQSSINKSLIYKVIFKKSTSYTKQLIYTYRANNLKPLPVTHVKGKRDIYNNLTISWIRRVRINGEWKDNIGTPIDEKFESYDIEVLDNQNIVKRTITIKDNSTFTYSATQQQQDFNSIQAKINIRIYQNSNVIGRGAVYEATL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSN0</accession>
    <name>MNMA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-specific 2-thiouridylase MnmA</fullName>
            <ecNumber evidence="1">2.8.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmA</name>
        <name type="ordered locus">Ecaj_0226</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47032"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:11728"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:87170"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.13" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304353.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSN0"/>
    <dbReference type="SMR" id="Q3YSN0"/>
    <dbReference type="STRING" id="269484.Ecaj_0226"/>
    <dbReference type="EnsemblBacteria" id="AAZ68275">
        <property type="protein sequence ID" value="AAZ68275"/>
        <property type="gene ID" value="Ecaj_0226"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0226"/>
    <dbReference type="eggNOG" id="COG0482">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035188_0_0_5"/>
    <dbReference type="OMA" id="AVCTGHY"/>
    <dbReference type="OrthoDB" id="1054741at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00144">
        <property type="entry name" value="tRNA_thiouridyl_MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="Adenine_a_hdrlase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="tRNA-specific_2-thiouridylase"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000349620" description="tRNA-specific 2-thiouridylase MnmA">
        <location>
            <begin position="1"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with tRNA" evidence="1">
        <location>
            <begin position="161"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="1">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="1">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Alternate" evidence="1">
        <location>
            <begin position="115"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00144"/>
        </source>
    </evidence>
    <sequence length="367" mass="40607" checksum="3692C5068E593127" modified="2005-09-27" version="1">MLDDFKIDPLVKNKSPSSTTVVVAMSGGVDSSVAAALLHKLGYKVIGITLQLYNNTSGKGGCCGSTDTQDAKQVASSMGFPHYTLNYEKAFREEVIENFIDTYTQGKTPIPCVKCNQVIKFRDLLNTTKSLGADVLVTGHYIRKVEQDDEIYVYSSKDTKKDQSYFLFATTIEQLKFLRFPLGNFHKDNIRKLAKYFNLSVAAKPDSQNICFVTDTYKNTIAHLRPQTVKKGHIIDTNGNILGEHNGIVNFTIGQRRGIGLASKEPLYVTKLNPDTNEVTVGPKSALLQNKLHIEDINWLLKDKIPYSGLSVKVKLRSSHSGSTAIIYPNDMNKATVILQDTYCTVTPGQACVVYDGDRMLGGGWIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSN6</accession>
    <name>Q3YSN6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304347.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68269">
        <property type="protein sequence ID" value="AAZ68269"/>
        <property type="gene ID" value="Ecaj_0220"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0220"/>
    <dbReference type="HOGENOM" id="CLU_592815_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="461" mass="51686" checksum="1484EE0A7D4135BA" modified="2005-09-27" version="1">MLNRTQNDNITQTLQGANNSYSPEENANNLNIEEQLINNPEIQVNPNIQAEEVIADAIQEPYNPNAQIEAANATEDNIINDTIEENDNNLNIEEQLINNPEIQVNPNIQAEEVIADAIQEPYNPNAQIEAANVIEDNIIINNTIEENANNLNIEEQLINNPEIQVNPNIQAEEVIADAIQEPYNPNVQIEAANVIEDNIIINDTIEENANNLNIEEQLINNPEIQVNPNIQAEEVIADAMQEPDNPNAQIEAANATAITNINAMVTLQNINEIYPEDPESLITQEERAIEYMIQKREQVLAEANIYNDISRPRVLIDHSAAMQDILKGYSIAKTQNKINNIEQEILRNTIIQAEKNVGILIENRKDLHVNTTQEEPNAQIETDITDAIQQEELEEPSNQSNDISNNSSHVSSDNTETVEVDIEAIPSLLRRIARDIVALINTELNDPNAEHVNTQNQNINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSP0</accession>
    <name>Q3YSP0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">DNA mismatch repair protein MutL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mutL</name>
        <name evidence="8" type="ordered locus">Ecaj_0216</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304343.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0216"/>
    <dbReference type="EnsemblBacteria" id="AAZ68265">
        <property type="protein sequence ID" value="AAZ68265"/>
        <property type="gene ID" value="Ecaj_0216"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0216"/>
    <dbReference type="eggNOG" id="COG0323">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004131_4_2_5"/>
    <dbReference type="OMA" id="AHERIMY"/>
    <dbReference type="OrthoDB" id="764332at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00149">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020667">
        <property type="entry name" value="DNA_mismatch_repair_MutL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="DNA_mismatch_repair_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="PTHR10073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="SSF118116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_mis_repair" evidence="7">
        <location>
            <begin position="208"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="MutL_C" evidence="6">
        <location>
            <begin position="495"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="682" mass="76800" checksum="00A87611F7DF52F0" modified="2005-09-27" version="1">MSIILLDPRTINRIAAGEVIECPASVVKELVENSIDANATTIDVAIERGGRNLIVVSDNGIGIKKEDIEIAFARHATSKLPDGDLAKVKSLGFRGEGLTSIAAVAKVKMISKYQDSDTAWLIVFEGGDKTQELTPDSLSCGTYMEVRDLFFATPNRLKFLKTEKAEVQSIIDMINKLAMVNHGIMFSLSVDNKQVFKYLRQQSNIDRLSEIKALGIEFCKNSLPISVKEEQIELSGYIGSPTLSRSKSSLIYTFVNGRPVYDSLLIGAVRYAYSDFIERDKYPVVVLYLNIPCDQVDSNVHPNKSEVRFQDKKLVYRVVVNAIKEVLSINMNAKLKSINEFESDHFVRDNIVSMNHSRNVGNDVTFELLKRLKAKQQPFNVQECNSTDMEANNLLDDNNSFCKNLKNVQSEYEVVYKNFEESVNCKKSERFNVFEEEKDAINLSNNALQDLSNVNDFNIGVVSGSMGLENDKLSQVSYKKIENGSLIDNHLLGYALCQIHSRYIVSQTQDAIIIVDQHAAHERLTYEYMKQVMEKEGIKRQMLLIPEVIEMNNHLDLELLVEYKAKLLKLGLLIEPLGNLSVIVREVPAIFGSFDVKSLIINIVDSIIEVGDTLFLDDKIKDICGTIACYSSIRSGRKLKIEEMNAILRSMENTAHSGQCNHGRPTYVELSLVELDKLFARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSP4</accession>
    <name>Q3YSP4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA translocase FtsK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ecaj_0212</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homohexamer. Forms a ring that surrounds DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FtsK/SpoIIIE/SftA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0212"/>
    <dbReference type="EnsemblBacteria" id="AAZ68261">
        <property type="protein sequence ID" value="AAZ68261"/>
        <property type="gene ID" value="Ecaj_0212"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0212"/>
    <dbReference type="eggNOG" id="COG1674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001981_9_7_5"/>
    <dbReference type="OMA" id="DYCIDDN"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025199">
        <property type="entry name" value="FtsK_4TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041027">
        <property type="entry name" value="FtsK_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002543">
        <property type="entry name" value="FtsK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018541">
        <property type="entry name" value="Ftsk_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13491">
        <property type="entry name" value="FtsK_4TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17854">
        <property type="entry name" value="FtsK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09397">
        <property type="entry name" value="FtsK_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01580">
        <property type="entry name" value="FtsK_SpoIIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00843">
        <property type="entry name" value="Ftsk_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50901">
        <property type="entry name" value="FTSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="FtsK" evidence="14">
        <location>
            <begin position="492"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="269"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="510"/>
            <end position="517"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAZ68261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="848" mass="95870" checksum="E595F657537B0241" modified="2005-09-27" version="1">MSMKSILNKTFFYLKISLLSFITAFVSLSIFTYHYDDLSFNIATDAPVKNWGGVVGSHVADILIQIFGVASFILIPLMIFYVVSVFYCVKRNLVLYLISNLITVIGVSGLVSSFKVMYKYHYGGVLGIYFQSCPIIVLLPIIAIGIVGVVGWQKILYFYDLLLNIPSRIINHVNNVNNSIIKKEVPENVGQVQEFYNAEEDSLNEEEELEDDLTDEFADKFTEGFDEGFTRELTEELMEGSIRESVSESVEELVEEFDFADEEELDFHAEEENEEDHNEENHPEVDDAQYDDEEEEEDSYDEYEGDNDDEKEDDYIEDEYDEERVVDSTPQMSFPVKLQEINPPMSTQIIREKKKSKEFILPSVDYLSKPNPINQRKFHPDDNVASLLDKVLKDFSIHGKIVNIRYGPVVTLYEFEPSAGTKSSRVIGLSDDIARSMSALSARISVIPGRNVMGIELPNHYREIVMLRDLFESAQYRDSKLKLPIALGKGIDGEVVIADLVKMPHLLIAGTTGSGKSVAINTMILSLIYSLTPDQCKMIMIDPKVLELSVYNSIPHLLTPVVTESRKAVAALKWVVSEMENRYRLMSDVGARNVVGYNDKIKEAISENRTLEKILQTGFDKETGEAIFEKVVIEPRIFPYIVVIVDEMADLMLVAGKEIESSIQRLSQMARAAGIHIIMATQRPSVDVITGVIKANFPTRISFAVTSKIDSRTILGEQGAEQLLGMGDMLYMVSGGRVIRVHGAFVSDNEVQDIVEYLKSQGTPEYIEGITQVQQDYDYCIDDNLPERDDELYQQAVSIVMRDRRTSVSYIQRQLRIGFNRAANLVERMERDGVIGIANTGKREILLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSQ9</accession>
    <name>Q3YSQ9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0196</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304324.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68246">
        <property type="protein sequence ID" value="AAZ68246"/>
        <property type="gene ID" value="Ecaj_0196"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0196"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031275_8_0_5"/>
    <dbReference type="OMA" id="GAYVKRY"/>
    <dbReference type="OrthoDB" id="944044at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="373" mass="41749" checksum="1D60B93C68B9FF17" modified="2005-09-27" version="1">MGEQLCKLIDKYVTRGVIILIIILAIFIIKPVFAPCCSAIIMAYLLNPLVNKLQNIGLSRQFSVAVILLSSVCIIIAFLISFIPIAYSQLLSLVRFLIERIPLIHKDSITAFLQKYNIVEYEEVSNVMRLSQLSFENLLSYENIKPLIGVLVGILKSLDNILFGAINSSISISYTVSIILITPILLFYILCNWPSIVEYSNSLIPVKYQSVAKLYTERIDKIISAYIRGQLSVCFIMGIYYTICLSLIKLKYFLIIGFISGIMTFIPYVGPISCVVLSFVITMLQFNSWTICGIVLVMFIIGQLVESNIITPLLIGKSIDIHPIWIIIGMITCGSQIGFTGILLSIPITAIVGVFVKSFISYYMGSEFYRNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSS0</accession>
    <name>Q3YSS0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BolA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304313.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0184"/>
    <dbReference type="EnsemblBacteria" id="AAZ68235">
        <property type="protein sequence ID" value="AAZ68235"/>
        <property type="gene ID" value="Ecaj_0184"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0184"/>
    <dbReference type="eggNOG" id="COG0271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_2_2_5"/>
    <dbReference type="OMA" id="KHADHYF"/>
    <dbReference type="OrthoDB" id="1981704at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="SSF82657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="97" mass="11307" checksum="234FF07ED7D8E3BF" modified="2005-09-27" version="1">MDIIQKIENKITSALNVLTLEITDESSKHNGHNFHSISNISHLKIQVVSDDFLKTSKINRHKRLHKILEEEIKLTHSISFSLYTKAEYQKLFFSITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSS3</accession>
    <name>Q3YSS3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phospholipase/Carboxylesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304310.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0181"/>
    <dbReference type="ESTHER" id="ehrcj-q3yss3">
        <property type="family name" value="LYsophospholipase_carboxylesterase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68232">
        <property type="protein sequence ID" value="AAZ68232"/>
        <property type="gene ID" value="Ecaj_0181"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0181"/>
    <dbReference type="eggNOG" id="COG0400">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049413_5_2_5"/>
    <dbReference type="OMA" id="GRQWYGL"/>
    <dbReference type="OrthoDB" id="1195966at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_2" evidence="1">
        <location>
            <begin position="14"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="213" mass="22950" checksum="9A3FE2AFCABCC003" modified="2005-09-27" version="1">MSIVIDGPSFISGDAANILVVLLHGRGATGDSIMSVGHLMSGLLLNAYFVAPNAPMKYGDTGYAWFNGRDFSEDVIFADMEKAALIVNNFIDLQLKNTGLTDDKLVLAGFSQGAMLAVHIALLRKRKCASVISYSGAIICPNYLKYKMNVKPDICIIHGTDDQVVPFSFFDAAVGFLSSNNVPIESHAISGLDHSINNVCIELSAKFITDRLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YST6</accession>
    <name>RL7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L7/L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplL</name>
        <name type="ordered locus">Ecaj_0168</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Part of the ribosomal stalk of the 50S ribosomal subunit. Forms a multimeric L10(L12)X complex, where L10 forms an elongated spine to which 2 to 4 L12 dimers bind in a sequential fashion. Binds GTP-bound translation factors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304297.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YST6"/>
    <dbReference type="SMR" id="Q3YST6"/>
    <dbReference type="STRING" id="269484.Ecaj_0168"/>
    <dbReference type="EnsemblBacteria" id="AAZ68219">
        <property type="protein sequence ID" value="AAZ68219"/>
        <property type="gene ID" value="Ecaj_0168"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0168"/>
    <dbReference type="eggNOG" id="COG0222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086499_3_0_5"/>
    <dbReference type="OMA" id="LEDKWGV"/>
    <dbReference type="OrthoDB" id="1822695at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_L7/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_L7/L12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_L7/L12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="PTHR45987"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="SSF48300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00855">
        <property type="entry name" value="L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000195792" description="50S ribosomal protein L7/L12">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00368"/>
        </source>
    </evidence>
    <sequence length="132" mass="14254" checksum="A6AFC3377F3F0BDD" modified="2005-09-27" version="1">MSTVDIDSLVEQICSLDLCKAAELVDKMEQKLGFPKGGLLTAVPAAGGGQAESSAAEEKTDFSVIFDSYAADKKISVIKAVRECTNLGLKEAKEFVEKEGAKELIEGKKYKKEEAEEIKKKLEDAGAKVIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSV5</accession>
    <name>Q3YSV5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpsU</name>
        <name evidence="6" type="ordered locus">Ecaj_0149</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304278.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0149"/>
    <dbReference type="EnsemblBacteria" id="AAZ68200">
        <property type="protein sequence ID" value="AAZ68200"/>
        <property type="gene ID" value="Ecaj_0149"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0149"/>
    <dbReference type="eggNOG" id="COG0828">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171685_0_0_5"/>
    <dbReference type="OMA" id="VEVIVYH"/>
    <dbReference type="OrthoDB" id="1995636at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00358">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001911">
        <property type="entry name" value="Ribosomal_S21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01165">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00030">
        <property type="entry name" value="S21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="27"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="91" mass="10964" checksum="F9E099A46F0DBEDE" modified="2005-09-27" version="1">MVEVIVYHGDIEQGIRTLKKKLQREGKPRQMKITHHEKPSEKRARKKDDCERRRKKLHKVPYEPQFTKFQVSARHFQVKRPSQVNDNSTNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSW2</accession>
    <name>Q3YSW2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0142"/>
    <dbReference type="EnsemblBacteria" id="AAZ68193">
        <property type="protein sequence ID" value="AAZ68193"/>
        <property type="gene ID" value="Ecaj_0142"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0142"/>
    <dbReference type="HOGENOM" id="CLU_128100_0_0_5"/>
    <dbReference type="OMA" id="IQYTVFG"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230985" evidence="1">
        <location>
            <begin position="23"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="178" mass="20755" checksum="9965811AB6C9E612" modified="2005-09-27" version="1">MRVSVILSAIVLYLMLNVGCEANTHDCLGLVYALNHCTSYACEMDFSNDKIQYTVFGLRNNNCKLMEKDASGMMLCYIPQDELKVMSEYLVRVITNNLNVDTYKIERMLFELCNFYYVMQDSLIPENEEVNEQNALQVTRAAELKRRNINISKIKSIFFRDEDIMKLHSIYAQNQNMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSW6</accession>
    <name>Q3YSW6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="3 13">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ecaj_0138</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NAD(+) = (R)-N(6)-lipoyl-L-lysyl-[protein] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11 13">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="13">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="3 13"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0138"/>
    <dbReference type="EnsemblBacteria" id="AAZ68189">
        <property type="protein sequence ID" value="AAZ68189"/>
        <property type="gene ID" value="Ecaj_0138"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0138"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_2_5"/>
    <dbReference type="OMA" id="WASMLND"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11 13" id="KW-0274">FAD</keyword>
    <keyword evidence="4 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 11 13" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 13 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 13" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="15">
        <location>
            <begin position="28"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="14">
        <location>
            <begin position="372"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="202"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="12">
        <location>
            <begin position="64"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAZ68189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="491" mass="53359" checksum="2108931C5FF998F1" modified="2005-09-27" version="1">MLHLQILLHSTIIIRFVSINKSMTKHEFLIIGSGPGGYIAAIRAAQLGYDVAIVEKENSLGGVCLNWGCIPTKSLLQSASVYHNIKKADIFGITVKDVKFDFNKIIERSRNVVEKLANGISGLMKKNNIKVYHGTAKLLGNATVEITDHSNKRISITSTHIVLATGSQAKNIPGIDFDNNIVWNAKNAMTPNKFPESILIIGSGAIGIEFASFYNTFGTQVTMVELKDNILPLEDHEVSKCMHNILSNKGIKIHTSSSITKLEKCNNYAKVQISDTIDLQVDNVILAVGIIPNSSNIGLENTKIKTDNAGFIITDKHCCTDEPGIYAIGDVAGFPCLAHKASHEAILCIENIAATENKIPQHKIHTINKNNIPSCIFSIPQIASIGLTEHQAKHQGYNFKVGKFNANCSGKAVAIDEVEGFVKVIIDKSTGELLGAHMIGAEVTEMINGYIIGKQVEITDQDITSAIFPHPTLSEMLHEAVLSSNNESLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSX4</accession>
    <name>Q3YSX4_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0130"/>
    <dbReference type="EnsemblBacteria" id="AAZ68181">
        <property type="protein sequence ID" value="AAZ68181"/>
        <property type="gene ID" value="Ecaj_0130"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0130"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_0_5"/>
    <dbReference type="OMA" id="FYMFTTY"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="25"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="441" mass="49030" checksum="90E63BF8F4A83195" modified="2005-09-27" version="1">MIASVLFINGRIVGGYLSYMNLRKAILSTIICNTLVWYDYVLFGNLVSVISKLFFPAEDRYFSLIMTFSIFAVGFLMRPFGASIFGYIGDKYGRKAALTLSIIAISVPITFISVLPTYDKIGILSPILLIICRLMQGISLGGEAGNAAFLIEHSKKGKNIGFFGSFETLSAVLGSIIALFMILLSQYFTGENFEIWGWRIPFVIGLLLGLISIYIRCITAESPAYDTHKENNNLSQSPVLELFKNYKRPLILATCIDCIENCSFHIFMVFFITFVKEFSSIHFSFNANTMSIIESFNIMICGILNVFFGYISDYVGRRRVMLIASVLLFCVAIPVFWLLSQGSYINLIVAYLIFVIPFSASLGPASGAMSELFPTKVRYTGFGLSRNIASAVAGGMAPVICTWLIRATGLIFIPGVYVMFWAVVAIIALCQIKKKDVYADW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSZ1</accession>
    <name>Q3YSZ1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Major outer membrane protein p19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304242.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68164">
        <property type="protein sequence ID" value="AAZ68164"/>
        <property type="gene ID" value="Ecaj_0113"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0113"/>
    <dbReference type="HOGENOM" id="CLU_1862034_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="137" mass="15847" checksum="0E31236F04BCFE2E" modified="2005-09-27" version="1">MLHVQNHVDQHTNHIEHDDYHFTGPTSFEVNLSEEEKMELQEVSSIDSVGCEDCDPNCRYPLELVECQRIEERPVCNAGLESLTVDAYQLGLLLGGFLSAMNYISYSYPCYYYDCCDRNYYDCCHKNACYYNCCDCA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT12</accession>
    <name>RL25_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L25</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">General stress protein CTC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplY</name>
        <name evidence="1" type="synonym">ctc</name>
        <name type="ordered locus">Ecaj_0092</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA. Binds to the 5S rRNA independently of L5 and L18.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304221.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT12"/>
    <dbReference type="SMR" id="Q3YT12"/>
    <dbReference type="STRING" id="269484.Ecaj_0092"/>
    <dbReference type="EnsemblBacteria" id="AAZ68143">
        <property type="protein sequence ID" value="AAZ68143"/>
        <property type="gene ID" value="Ecaj_0092"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0092"/>
    <dbReference type="eggNOG" id="COG1825">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075939_0_1_5"/>
    <dbReference type="OMA" id="HVDFYEV"/>
    <dbReference type="OrthoDB" id="1673077at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00495">
        <property type="entry name" value="Ribosomal_L25_TL5_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01334">
        <property type="entry name" value="Ribosomal_L25_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029751">
        <property type="entry name" value="Ribosomal_L25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020057">
        <property type="entry name" value="Ribosomal_L25_b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037121">
        <property type="entry name" value="Ribosomal_L25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001021">
        <property type="entry name" value="Ribosomal_L25_long"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01386">
        <property type="entry name" value="Ribosomal_L25p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14693">
        <property type="entry name" value="Ribosomal_TL5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00731">
        <property type="entry name" value="bL25_bact_ctc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000244209" description="50S ribosomal protein L25">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="209" mass="23055" checksum="9643D462B6E92795" modified="2005-09-27" version="1">MTDQGIVKINASLRENVGTGPARAVRRNGGIPAVVYGKNRDSLSVFLSDREFLSKHRSAALSTHLIELEIGDKKEYVLMRDVQKHPVTDRIQHVDFQFIDYGTEIKIEVPLIFTNEQKCIGVKRGGVLNILHRTLSIKCLPNAILQNIEIDLSDLTAGHSIHVSDLNLPPEINVVMKEHNPAIVTISSTSMEKEGEGSQEPTAAPSSEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT18</accession>
    <name>SSRP_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SsrA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Small protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">smpB</name>
        <name type="ordered locus">Ecaj_0086</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">The tmRNA-SmpB complex associates with stalled 70S ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SmpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304215.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT18"/>
    <dbReference type="SMR" id="Q3YT18"/>
    <dbReference type="STRING" id="269484.Ecaj_0086"/>
    <dbReference type="EnsemblBacteria" id="AAZ68137">
        <property type="protein sequence ID" value="AAZ68137"/>
        <property type="gene ID" value="Ecaj_0086"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0086"/>
    <dbReference type="eggNOG" id="COG0691">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108953_0_1_5"/>
    <dbReference type="OMA" id="WTNHSAR"/>
    <dbReference type="OrthoDB" id="1720952at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070929">
        <property type="term" value="P:trans-translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09294">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00023">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023620">
        <property type="entry name" value="SmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000037">
        <property type="entry name" value="SsrA-bd_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020081">
        <property type="entry name" value="SsrA-bd_prot_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30308">
        <property type="entry name" value="PTHR30308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01668">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74982">
        <property type="entry name" value="SSF74982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00086">
        <property type="entry name" value="smpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01317">
        <property type="entry name" value="SSRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_1000002049" description="SsrA-binding protein">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00023"/>
        </source>
    </evidence>
    <sequence length="148" mass="17352" checksum="BDD07876BBC77EB8" modified="2005-09-27" version="1">MDVIIENRKVRFNYFIIQEFDAGIVLIGSEVKSLRQRKVSIAEAYVTEKNMELWLCNLHISEYNHASTKNHNPTRPRKLLLRKKQIYKISGNIKNDGFTVVPLSLYFNEKGLAKAKIVIVKGKKLHDKRETIKTRDWNREKSRVLRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT32</accession>
    <name>Q3YT32_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304201.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68123">
        <property type="protein sequence ID" value="AAZ68123"/>
        <property type="gene ID" value="Ecaj_0072"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0072"/>
    <dbReference type="HOGENOM" id="CLU_317785_0_0_5"/>
    <dbReference type="OrthoDB" id="2105628at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021902">
        <property type="entry name" value="DUF3514"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12027">
        <property type="entry name" value="DUF3514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="916" mass="101866" checksum="0E6EA30E6E576C44" modified="2005-09-27" version="1">MLVGIFIAGAAAWAIVVLCKSRKTGNKSNIDNVETSTSGAAATTSDGELSEELSSLDIASSKSRVLDREGGGGTSLEENVVKLSGKSSRKRNKKKSTLKESKNVVPDIDVSSDVNGISGANSEKEEGSSRTEEQAANLDAKYLRQGAEPKVRQRTSIKQTKAGEKTGALQSSVKSSSQKSTKTQDVESKTSDSTVECFAKVEDISNSGNTSTMSKDDVTVSVESVSSQHLRTRNTEGKISSHQVGQSSDVESTGDQHASKKKGKKKKKKTLSVLRGVSDETLDTVHHTSGAFESTAGVFNPASEDTEGSLEQEYLRQGAKPKMRQPVASRQTKGAKKTDIVQSDVQKDVSARSSDILSNVKQDLKSSFPKSFDRSNTKARSVESGERQGVPSESAVKFVTKTLAEADLRVKESEIRKSSQRGKAQGLHGVSSKPCGEGSIEVVDYREEKRRARVEDVQGFFGGYLKTSTGKERESRSLEDVTVVEYVRREGDCGVVSREDRKSEVDNGYLQQGAKPKICRSYSIKQRKDTDAIPSCVHREVESSEKLPGVSEGQKTQVLTSVEASTHMTIKSFIRKHTSHLSAKEYFQQMVKFRKTVEQFADMHFVYSANFMFNIFNIVGNRLVLKPEIQNSLLKVTNNINLCTFIIKFGIIKQMVGRLCNLVILKYFKYCASHYYDQSHMDAFLISLINLGFSDLKTISARVCNFLYCYSQELKDKAVFKVHSDSFYYDMLGMYIDIVNSRVQDTQLNDLIDLIKVSCIVQFGHMYHIIVQSKSITTESCTDKEIQALIFQERSVKHMLFYAVRFARICDNARMAFSHLYSPDEKRNPVCVTSGLAFPRNIIRPCREIINDIDHNVKNGTMHFGVFMSNIANYTTSLFLNSELEFLIRKDIETYRKVIAEQYLTMGSAAESSRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT36</accession>
    <name>Q3YT36_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304197.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68119">
        <property type="protein sequence ID" value="AAZ68119"/>
        <property type="gene ID" value="Ecaj_0068"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0068"/>
    <dbReference type="HOGENOM" id="CLU_396260_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021902">
        <property type="entry name" value="DUF3514"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12027">
        <property type="entry name" value="DUF3514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="616" mass="70535" checksum="FE3DF0D159AA7B20" modified="2005-09-27" version="1">MKKKKKKKKKKKEQIKSSDEKSEGIGEHNQCQSTMCDIKTSSKRNAEVNATGNLHNHSGEEYLTTSEVSDAIQSSDLQTEYLRQGARPKVYSSKPNDTKETNYIEKSAVGFYDWSSKSYKEIEPLLVQVKKRSEVGNIQSTASVTQSQSTKEELLKSNNVKIKQVSGKKLTFNHVLLEADKANEQLVKVSEMEYLGSIIPKNKTLKSTKCTQYDLEDTFLYDVVDYEQEQEIVKMFKQVGIEDVDQSLIGVDSCDVSVSGSSVKRSKSDISQGLSICIESLKMSRITVSQTVSDSRSTEKLLRKGNVIRSNGVKYRGRMQKQYQSRLYNFMGMHFNYNCNIIKEIFCVQNNKVLLRNNIMNFLVHEVSNYQLCSFVLRLYSVLHCTLLLNRYKFCDKLIYNRYITTSALDMCIVDLLANIQNTSMSFYRLKEIIHYHHVQKFPTVMGYYSSNFFSDILKLLRNLSSVKPGSTNYSRRVLNFAIVSCAMILGSMYSKIEVVTSKARVGEGKHTEVFGLMCYYVLYKMQYMYDVDSNVTVVIGNLCFPSLLVEQCSVNFLDIAKVKIANDNMSFDVIMNDLANNICLLMTKDNLAAFEQHIREYSYIYDLDRCRGNTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT42</accession>
    <name>Q3YT42_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304191.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68113">
        <property type="protein sequence ID" value="AAZ68113"/>
        <property type="gene ID" value="Ecaj_0062"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0062"/>
    <dbReference type="HOGENOM" id="CLU_308309_0_0_5"/>
    <dbReference type="OrthoDB" id="2105057at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="957" mass="106812" checksum="9B926EA86ECABBCF" modified="2005-09-27" version="1">MLSSIASLKEDIENIAKSIEELISSYNVPNRKLSLKEAVRLEKICVLKSELDEVLHIGLYGSKDLVLEQPVFGYQQRNQGKIEFDKEHMLLVSMRFSRLILYFLKVEELLIRFSDLRSEGRAESNSVCANMDNDVNLLIQELSKRGKLLSKVLGGKSDEIEAKVESLEQNEITIHSKAVDSIGDQSNVQVSLISKESEVELLSDVNNPKKKKKKKQSKQKLNVQNNLVLDSVELGSSEVMASVQNEGSEHSQTVDSVGGESDVQEQCVGLEESSINDLMKQEKAELLVQKPHALSINFEPNISSDQLSKEQSVELFEKAWNELTVLPQDMQLKHEEDLKSKELMARSSVIVERDEYLRLGARPKYSSVVDNVVNRQHSDQVSGVRLKSEKSSTGIQLREVNTKQYKQEKSKSLVSERSEMGSSDLIIKAKSSKKNTRQLNNSTGDVVSSQINEKLQNVDLPSKASKGDMQLSGNSRKNAQLANSGTGIADSQLSDKVHNIDLPSKASKGDMQLSGNSRKNAQLANSGAGIADSQLSDKVHNIDLPSKASKGDMQLSGNSRKNAQLANSGASIADSQLSDKVHNIDLTSRESKESIQLSEVDKQRTAQGKLATNVVSQSSDKLSYDTQGNVLKTKQSAQVELVSVDQIVISGKCLPSGYEVLNSSVRVFQDMHCNYCINMLQKIFDLEGNKIVVNSDIDLLLQNVFHGNKKTCVFLIKNSILVQFFSMFGIEFLSIFVGSKLKEGTVDKMLVDILTVIKNGVDIHYNIYKILCKFVVLKKVPCQLELAWFHDNMRILYSAVNALKDKSADEELMNFIAVSAVMSYGVLRLGKNMICNYDVQLNKYFMESCKQYCECDLGIQAYNILFAFGPLYCLETYGVALVRDLYVPQSVMMACCRQNSCNVVASVEKRDISFSVFIQEMVKSFYPLIDRNAISHYIVSIMQCYKNVVQKESCNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT44</accession>
    <name>Q3YT44_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304189.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0060"/>
    <dbReference type="EnsemblBacteria" id="AAZ68111">
        <property type="protein sequence ID" value="AAZ68111"/>
        <property type="gene ID" value="Ecaj_0060"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0060"/>
    <dbReference type="HOGENOM" id="CLU_224436_0_0_5"/>
    <dbReference type="OMA" id="VINNCFT"/>
    <dbReference type="OrthoDB" id="2107616at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1714"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1837"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1928"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2028"/>
            <end position="2070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2087"/>
            <end position="2163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2333"/>
            <end position="2353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1719"/>
            <end position="1733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1843"/>
            <end position="1857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1939"/>
            <end position="1957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2087"/>
            <end position="2112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2113"/>
            <end position="2138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="3714" mass="422339" checksum="15DA8D8C95235A2C" modified="2005-09-27" version="1">MYQQQPSIESIKGLLNNVFEEVSEVSIDYVKMWEYIYWACLIRSDDYLDTINLVRQCDKEHDIFHHAVMCNPEVFISEIAKPKFSSVLQSIKDNHEDCKTIELCLLNGYALSVIVADPLFEEFRLKVTSIRIGGINLCQDIYNEVKEKNSRRGVDKCSEEDVDFLVRGVMYESHIKKYLHLFPNLIYLDCVVAEQKCCKVLFKTLKHYTPKLYYLSLKCANAVPAVKWEIGEEFNDIQLKVKELLLVNCSMDLLLYFLEIESLFIIAPQNLPEDRKILLHDYYYNPDSVVQLLVSTNSYMVDSDGKISCVPVDYDIMSFQKLEVLAINPCQDMSSILYMDGLWDTEAGLNFVDQLITSRKYNLLSGLVDSHPSLLRKLFFVTSPIHKIDLLEDNEELFPKLVPCHFKNGASLIKSLGVGYLIPHFSYSKFGASKRDLKLIQLRLKITKALKDFESELFDNEAIYGDIIKYITRLAYDANGIGSDQSASDKFLKNVLSEAHFYDSVSLGYLVKIVSVNFPTVFCGEFFGSLLDYEESLHIYLVKGIETFIRRKFVEGSIKTLADLKDALLVFQKSAHGKANVAGTNEPVSRILDAILAGPIKQNVQNPHVSAKALCISKLDLYAKEILNDVVGIANVLQIDKSRKITSFVESTLCKTIPCIISNITRTIDNTYASLADDFWEDIFASYNDEFIKMLPNNYQFCTALVTCSTIVKNIGNASSYVRTFLLLDEVGIKLVELRYDYMKLRMLKLSRYSNKMFASLAVLTFFRQVLNFSGMSDEKIRDYLSFCGVKDFASMIIVISNLAFNKLRNFNEQKLIADHRDLGFYEDLNQAFIQHKISHEFMLKILSDVLANTITEVMRLYELHLVPLLEKKVNRLIGVIGNPEDMLQMKNVISSYIMSIRDVFIVKNDLYHFVDDEIVDSDVEMFDRIQSFSPISRKGLLDILNVMFCAKNADVDLLIAQQSSCVLMYLQPMMQGVSFSFKSLNSYRFIYRAINTMYIPYENKYKYSFTRLQSISNVPIVLVSLLFQKIFYSYAKDTIDRQLLFKILYKFQHYFDFQFIQYCFILPQVLENDANVFIYVKQSQNLVQVLRQVEPEVIISTIDNVVRSEVDITELYNTVLSSLILLNKSAVPELSSKEKDYFSKMIDLFQNNFETHGILDGDVMRIISIKLTFEDWSIPQSRCVSDCMVANLSNIKVLKSIQECCDLWVVDLRNQILECEGFPNLNVLDIRNDLVNKCKNVDGINDLTYQLILDKLKPSILPVQYLDEIACCIVLDLVQHIVDNEHINVFYERLVDALVSRKKLSTMQNICETVLQTLKGFLLNKDEFLIGVSSHRQTSLEKEEQTSADSLLSQCFAEWCYVILENLFKGKDLANSVKSYSIAQFVSDNGLPESALLDLEKRMKQRQQVDKYLRLIDRFKSICMRNNNVGIQDRVDTRSRQIFNIENSQIIPLMQKKLSMNKDNTDDNISRLLNDDQYDFSTSQGKSTQILKIIAMMFYSLKPCNKQPVSVHQYVCDNVYISLMKISLISVFFDKGIISGFEIISVVRVLLNMFSSNPYFVNMRYLDFSENIMNYVTEIQYGDFEDKTPRKICNLGFHLVWEMLGVLLHDLLLNCLSDVDSSLCYAEDYFKRLNNIDDYTMLLVKFYYRNVLLRYSEQSSVDSTLKFHPVVFLCKFLKVFDYDLLKYYLPECNITLPVINGSKGKVTDVADTHSLKGGSELPELDEKSVSKKQRSRKKSKKKEEKIYANVVGDPELMYCDETAKEFLISRCVNDLSEAVAYITEVKGSDHLVVKEENFAGVTDALSKSQGPSEPEYNITLHVINGSKGKVTDVTDTHSLKGGSELPELDEKSVSKKQRSRKKSKKKEETIYVNVVGDLELMYCDETAKEFLISRCENNLSEAVIYIPEVKDSDYLVVKEENFAGVTDALSKSQGPSDQVENEEVRQDKGSEVQKRSKKSNKKAKKSAVSKDKIGSSVPEKVDILSVDESVSNRDDLEEKTASDVKVADTLPKQEKETSIIVTETLANKEQESSNVLTSEQVLKKAEDKAQKNSKKGNKKAKKGAVSKDKIESSVLEKVDILSVDESVSNRDDLEEKTASDIKVEDTLPKQEKETSIIVTETLANKEQESSNVLTSEQVLKKAEDKAQKNSKKGNKKAKKSAVSKDKIESSVLEKVDILSVDESVGDRDDLEAKTANDIKVEDTLPKQEKETSIIVTETLANREQESSNVLTSEQVFNKKISEVQYDSVAGKKIAESKENELVELVSIVEESINESLNHEDNLEGDVIDGCVTGFKSSEILLIQNENSDGQELPTRSLSRKASKKARKQALKMQEDCQKSKKKTQKNTKHNNARKIVKAETESTSILEDQYSSVSTASKRPISNVVVQSSDIGQDCRQLQVNYIEICDEILHTHRSDLEKLFGSYDEERLRVIIESISVKNLISQKIINLEMKRMKRIATSNTGLNVVMPSLEYTSSLCRIRSKSMCDLRFSHFLYSVQLIDSLFEVVNSNSGSRKVVVKGTAHRVLKNVFGYRKDYNFFVILMKVFVLLNLSAIAISHSDVNLRRINVYFKHSCDVFKMDLNPILLEVIQVLESIKGKEIDLYQLQNCMSGIFCKFFKESMPNDNYSLRFLKSLLSILSIITVDTALANDNDLRIVRDAAMFGCIIYIGSMFQQYTVQYSKLEQDNHPFKQLTCQDRSFWFMQEDYKKVVNLIKLFNTFRNRLVHCYDSENERFAERFKALSSQGRCFNSKYDIADILPTDKHKCYHAWHDTSDFFATLSITVLQTELLLINDSDCDSLGYSFLNRIYAFFQRCNMDIYPYALSERVNVDTDKMLALIRLRTALSTFCSNSASGFETSRHYILSLNQTLEKMQYCCDNRLFSTCIKKYTVMSKLLEQERAISFDADSMTDTSPIHDGVNNFQYDGNSDVLKKHDVRFDDVPEQDTVVDVKLEMSTDTDLVESMRDDSLSVSVDCDTSFIGSTFEQRNYTPTVDLKVPSCSRSFDIEDDSRLANVTRQGNSSAMLEAPSYSGLLDVESSITGNYTSNARLEGVPSYSMQNAQCANNLLRSVVNNSEVADLPVQENLPFSSNLVGDVQYADNLTVSYHSDTDLINLSKRRIRGYKRPKSNIQNTYNPLVCANDSDFMKSAYTHQKNRTTYDARLGMHDYNSSVKIMQPSYNLPSSQLSENPQVRDYATLGARPETHYGSQQKKDVQHTTNLPNIGNNSSSVINNCFTQGYSTFSNISESDMRYVDNPLVSEKPAVSLDGQHYLTRNARLGMHDYNSSVKIMQPSCNLPSNQLSENPQVRDYATLGARPETHYGSQQKKDVQHTTNLPNIGNNSSSVINNCFTQGYSTFSSISGSDMRYVDNPLVSEKPAVSLDGQHYLTRNARLGMHDYNSSVKIMQPSYNLPSSQLSENPQVRDYATLGARPETHYGSQQKKDVQHTTNLPNIGNNSSSVINNCFTQGYSTFSNISESDMRYVDNPLVSEKPAVSLDGQHYLTRNARLGMHDYNSSVKIMQPSYNLPSNQLSENPQVRDYATLGARPETHYGSQQKKDVQHTTNLPNIGNNSSSVINNCFTQGYSTFSNISGSDMRYVDNPLVSEKPAVSLDGQRYLTRNTRLGVPNYDDSVGMMQCSGSLPRCQVGNYPTVITNPNVQQHATSLNTRLSLHVYDCSVESVSHDDLQVDDLMNSIANPCVQGCCYPAGYKYMRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT54</accession>
    <name>Q3YT54_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304179.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0050"/>
    <dbReference type="EnsemblBacteria" id="AAZ68101">
        <property type="protein sequence ID" value="AAZ68101"/>
        <property type="gene ID" value="Ecaj_0050"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0050"/>
    <dbReference type="eggNOG" id="COG0767">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045686_1_1_5"/>
    <dbReference type="OMA" id="NYLVFPK"/>
    <dbReference type="OrthoDB" id="1253502at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030802">
        <property type="entry name" value="Permease_MalE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30188">
        <property type="entry name" value="PTHR30188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02405">
        <property type="entry name" value="MlaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="265" mass="29242" checksum="962F28195F0209B4" modified="2005-09-27" version="1">MQFLRLHLGLFWLLETVGKYVINLIFNIGHSFVFFVKFVCNCFMPPYYYGVIFKQFIEIFFFSLPIVGITAIFTGAVLILQNSLIIHNNVSGDFVSGVVVVAIVRELGPVLIGLIIAGRVGAAIAAEIGTMRITEQIDALFTLDTNPFKYLIVPRIISAMIAMPLLILCADLIGAYGGYIVGSYQLGYTPEIYVRGIMKFLHVKDVILGLIKATVFGFIISFMGCYSGYYCSGGARGVGMATTYVVVVSSMFIILLNYVITVFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT60</accession>
    <name>Q3YT60_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type-4 uracil-DNA glycosylase</fullName>
            <ecNumber evidence="3">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0044</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 4 (UDGa) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304173.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68095">
        <property type="protein sequence ID" value="AAZ68095"/>
        <property type="gene ID" value="Ecaj_0044"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0044"/>
    <dbReference type="eggNOG" id="COG1573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044815_1_0_5"/>
    <dbReference type="OMA" id="RPWLIAE"/>
    <dbReference type="OrthoDB" id="1529383at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005273">
        <property type="entry name" value="Ura-DNA_glyco_family4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00758">
        <property type="entry name" value="UDG_fam4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="9">
        <location>
            <begin position="100"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="261" mass="29623" checksum="4E5323D8B2ED5543" modified="2005-09-27" version="1">MDDLDLLEVLKFYHENGVDCTLEDIQYHVLKNKNLSLQDTDSIKSNLEGKKISMKENTETYYLLKAQSLAEKCNNIEQLKSAIESFDGCDIKNLATNTVFADGNPKAEMMLIGEAPGANEDLKGIPFCGASGMLLDKMLKAIGFDRTTVYISNAVFWRPPGNRRPTDFEIAVCKPFVEKHIALIIPKMLVLVGSTACYAMLDSKNPISKLRGRFHLYNNQFLQNSITTSIIFHPSYLLRQPMQKRIAWEDLKQIKRYFDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT80</accession>
    <name>Q3YT80_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TPR repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0024</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304153.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0024"/>
    <dbReference type="EnsemblBacteria" id="AAZ68075">
        <property type="protein sequence ID" value="AAZ68075"/>
        <property type="gene ID" value="Ecaj_0024"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0024"/>
    <dbReference type="eggNOG" id="COG4976">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_810491_0_0_5"/>
    <dbReference type="OMA" id="FVEHWLI"/>
    <dbReference type="OrthoDB" id="1865374at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltranfer_dom" evidence="3">
        <location>
            <begin position="188"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="356" mass="40861" checksum="2730E752B1018E85" modified="2005-09-27" version="1">MKSNFFITKCNVSSLISSINKAIPKLQDLLQLINNKIICMKKETILLQKKLKNLLETNIDLGLYHFYKGNISDAKFRFRLISIFKPKLPIAYYNLGRCYFALQNINKAKQNLTRAIELDNNYAEALYYLNKITNPTSIVYVPENIIKQYFDYTSEHFVEHWLIAKQYKAHEYVKSLIINFFGDKSPYLNILDLGCGTGICGQFLKMKSIGNYITGIDLSNKMINIARGCFVNGKQAYNELIHISIADFLKKNQDKKKYDVIILTEVLQYIGSLNPIFKLLKTMLDTNGIIIGLTRRKQGSGFQFINEGDFFCHSDEYIKLCIEESGLECSYSSYCKIYGSQVEGVLLVAQSKKIDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT82</accession>
    <name>Q3YT82_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">VirB8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0022</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304151.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YT82"/>
    <dbReference type="STRING" id="269484.Ecaj_0022"/>
    <dbReference type="EnsemblBacteria" id="AAZ68073">
        <property type="protein sequence ID" value="AAZ68073"/>
        <property type="gene ID" value="Ecaj_0022"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0022"/>
    <dbReference type="eggNOG" id="COG3736">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068461_1_1_5"/>
    <dbReference type="OMA" id="DLIQVRY"/>
    <dbReference type="OrthoDB" id="887933at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030255">
        <property type="term" value="P:protein secretion by the type IV secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007430">
        <property type="entry name" value="VirB8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026264">
        <property type="entry name" value="VirB8/PtlE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04335">
        <property type="entry name" value="VirB8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003299">
        <property type="entry name" value="VirB8_PtlE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="VirB8" evidence="6">
        <location>
            <begin position="26"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="234" mass="27462" checksum="3BEB5FD88C58FA1D" modified="2005-09-27" version="1">MFNKFKKKNNTSDNKKPLKANLETTYSWHVSRYNSVVIQRNMLLFFTVLALSAVGISVFVISNISKSRTIEPFVVEIEKKSGITTLVNPVSVKQYSADEVLNNYFIIEYVRSRELFDPNNFQYNYYTKVRLFSNQTTYSEFRNWIRLSNPASPLNLYANVTSGYLKIRSLQHLRPGNVQIRFSLEFNHPNGTIKRDRIATLSFQYVTLEMNEQERQINPLGFQITYYRADDEFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT89</accession>
    <name>NADA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Quinolinate synthase</fullName>
            <ecNumber evidence="1">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadA</name>
        <name type="ordered locus">Ecaj_0015</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dihydroxyacetone phosphate + iminosuccinate = H(+) + 2 H2O + phosphate + quinolinate</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:25889"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the quinolinate synthase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304144.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT89"/>
    <dbReference type="SMR" id="Q3YT89"/>
    <dbReference type="STRING" id="269484.Ecaj_0015"/>
    <dbReference type="EnsemblBacteria" id="AAZ68066">
        <property type="protein sequence ID" value="AAZ68066"/>
        <property type="gene ID" value="Ecaj_0015"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0015"/>
    <dbReference type="eggNOG" id="COG0379">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047382_1_0_5"/>
    <dbReference type="OMA" id="CFCSTMN"/>
    <dbReference type="OrthoDB" id="613347at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00568">
        <property type="entry name" value="NadA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023066">
        <property type="entry name" value="Quinolinate_synth_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="PTHR30573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="SSF142754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000061154" description="Quinolinate synthase">
        <location>
            <begin position="1"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="115"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="201"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00568"/>
        </source>
    </evidence>
    <sequence length="313" mass="35233" checksum="80BD79E95EE318A1" modified="2005-09-27" version="1">MKEVDIIKLSQEIRHLSKENNAVILAHYYQDSEIQDIADFIGDSLELSRKAATTTASIIVFCGVYFMAEVAKIINPTKKVLLPDLKAGCSLADSCDAKSFKKFRELHKDCVSITYINSSAEVKAYSDIICTSSSAEKIIRQIPEDKQILFAPDKFLGGFLEKKTNRKMILWPGTCMVHESFSERELIDMMVRHDKAYVLAHPECPDNLLRHSHFIGSTTQLLKFSAERPNSEFIILTEEGIIHQMKKLSPGSTFYVVNTLDGGCASCNKCPHMRLNTLEKLCLCLKNGYPEVTLDTEISNMAKKSLDAMFKMT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT99</accession>
    <name>DNAJ_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaJ</name>
        <name type="ordered locus">Ecaj_0005</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304134.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT99"/>
    <dbReference type="SMR" id="Q3YT99"/>
    <dbReference type="STRING" id="269484.Ecaj_0005"/>
    <dbReference type="EnsemblBacteria" id="AAZ68056">
        <property type="protein sequence ID" value="AAZ68056"/>
        <property type="gene ID" value="Ecaj_0005"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0005"/>
    <dbReference type="eggNOG" id="COG0484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_7_5"/>
    <dbReference type="OMA" id="DLHCTVT"/>
    <dbReference type="OrthoDB" id="1738789at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="SSF49493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_1000085191" description="Chaperone protein DnaJ">
        <location>
            <begin position="1"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="5"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="152"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="169"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="191"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="205"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="1">
        <location>
            <begin position="139"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01152"/>
        </source>
    </evidence>
    <sequence length="382" mass="41952" checksum="8429B6544DD2034B" modified="2005-09-27" version="1">MSKSDYYELLGVSKNATSEEIKKAYRKMALKYHPDTNPGNKEAEEKFKELSEAYDVLIDQDKRAAYDKYGHNAFDGAAGRGGFDFNSGFSGDFSDIFNDLFGGGFGSRGGRGSSRRSEGAAGSDLRFDVEITLEDSFNGKKVPISYVTYVKCSSCSGSGSEGSAKSVQCNTCHGAGSVRTQQGFFTIERTCHVCNGEGEIIQNKCKKCSGSGRVRDEVNLLVTIPKGIESGNKIRLNGKGEAGYRGARSGDLYVYSNIQKHKFFTRSGPDLYCTVPIKMTLAALGGHIEMPSIDGTWTKVKVPEGSQSGDKLRLKEKGMPVINSSKRGDMYIQITVETPVKLTKKQKELLQKFDDEPNVDCNPQSTGFFQKVKSFWKDIRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQN0</accession>
    <name>Q3YQN0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 13">Aspartate-semialdehyde dehydrogenase</fullName>
            <shortName evidence="13">ASA dehydrogenase</shortName>
            <shortName evidence="13">ASADH</shortName>
            <ecNumber evidence="8 13">1.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Aspartate-beta-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">asd</name>
        <name evidence="15" type="ordered locus">Ecaj_0944</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-L-aspartate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 13">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 13">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="8 13"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305048.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0944"/>
    <dbReference type="EnsemblBacteria" id="AAZ68975">
        <property type="protein sequence ID" value="AAZ68975"/>
        <property type="gene ID" value="Ecaj_0944"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0944"/>
    <dbReference type="eggNOG" id="COG0136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049966_0_1_5"/>
    <dbReference type="OMA" id="CEEEMKM"/>
    <dbReference type="OrthoDB" id="1799040at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02121">
        <property type="entry name" value="ASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000319">
        <property type="entry name" value="Asp-semialdehyde_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012080">
        <property type="entry name" value="Asp_semialdehyde_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005986">
        <property type="entry name" value="Asp_semialdehyde_DH_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01296">
        <property type="entry name" value="asd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01103">
        <property type="entry name" value="ASD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="12 13" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="13" id="KW-0521">NADP</keyword>
    <keyword evidence="13 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="14">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="13">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="39"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="160"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAZ68975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="336" mass="37301" checksum="E18D260EB27E6079" modified="2005-09-27" version="1">MNYKIAVVGATGNVGRVILNVLSSRSFPAKEVIALASKKSVGKKLSYGDKILESQDLDSYDFFDTDIAIFSAGSSISQQYARTAANKGCIVIDNTSLFRMEDEIPLVVPEINPNDISQYHKHNIIANPNCSTIQMLLALHPLHKISKIKRVVVSTYQSVSGAGKSSMDELYSQTKGTFMNQSIAPKQFTKRIAFNCIPHIDIFMKDGSTKEEWKMAVETKKILDSNIEVSATCVRVPVFVSHAESVNVEFFSEISEEEAYEALENAPGVLILDRREDGGYATPLDCVHEDEVYVSRLRKDNTVKHGLNMWLVSDNLRKGAALNAVQIAEILIKDYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQP3</accession>
    <name>TRMD_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine-N(1)-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmD</name>
        <name type="ordered locus">Ecaj_0931</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates guanosine-37 in various tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA methyltransferase TrmD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YQP3"/>
    <dbReference type="SMR" id="Q3YQP3"/>
    <dbReference type="STRING" id="269484.Ecaj_0931"/>
    <dbReference type="EnsemblBacteria" id="AAZ68962">
        <property type="protein sequence ID" value="AAZ68962"/>
        <property type="gene ID" value="Ecaj_0931"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0931"/>
    <dbReference type="eggNOG" id="COG0336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047363_0_1_5"/>
    <dbReference type="OMA" id="ILCGHYK"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N(1))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18080">
        <property type="entry name" value="TrmD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00605">
        <property type="entry name" value="TrmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002649">
        <property type="entry name" value="tRNA_m1G_MeTrfase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023148">
        <property type="entry name" value="tRNA_m1G_MeTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016009">
        <property type="entry name" value="tRNA_MeTrfase_TRMD/TRM10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46417">
        <property type="entry name" value="PTHR46417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01746">
        <property type="entry name" value="tRNA_m1G_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000386">
        <property type="entry name" value="tRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00088">
        <property type="entry name" value="trmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000257416" description="tRNA (guanine-N(1)-)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="134"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00605"/>
        </source>
    </evidence>
    <sequence length="235" mass="26333" checksum="F0E5704BBB6BCF2B" modified="2005-09-27" version="1">MTLNVNVLTIFPEMFPGPLSYSVIGRALNKGIWNLNVIDIRSFAKDKHKTVDDKPYGGGPGMIMKADVIGSAIDEVLSKNKNTKLIYMSPSGVKLNQDISGQLTHFSNITVLCGRFEGIDKRILDFYDFHEISIGDYILSGGEVACMVLIEACVRLIPGVVNNTQSICDESFTLNNQLEYPQYTRPAKWRGLEVPDILLSGNHKKINLWKANQSYCITKQRRPELTDTINGDIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQQ0</accession>
    <name>Q3YQQ0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0924</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305028.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68955">
        <property type="protein sequence ID" value="AAZ68955"/>
        <property type="gene ID" value="Ecaj_0924"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0924"/>
    <dbReference type="HOGENOM" id="CLU_125881_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="144" mass="16220" checksum="6C30BBFF06778E25" modified="2005-09-27" version="1">MNNGYNYHYHAHQVDFLIQKSDSPNIDFYHGVLHFNDEDSRYFATAEVINDLTGDKGTINILEYEDGMYYDDGHIAKCDLGILLEHYDSVKSINEVTSINRAFLPTLLGNLDQLLIDPSKVDANEAAITVLIHNGVNSQMISLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQQ5</accession>
    <name>Q3YQQ5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0919</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305023.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68950">
        <property type="protein sequence ID" value="AAZ68950"/>
        <property type="gene ID" value="Ecaj_0919"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0919"/>
    <dbReference type="HOGENOM" id="CLU_2045982_0_0_5"/>
    <dbReference type="OMA" id="NIFDNTE"/>
    <dbReference type="OrthoDB" id="1995513at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="120" mass="13814" checksum="453BEBCA15A31BA2" modified="2005-09-27" version="1">MDMINIFDNTEDDAFSVSNFINQNFISQFTITILPPSVPLYHDQHIDEGMYSVVFSYKKYEAQQPYGLVEHKSGKFEASLDHSDHRLYLNKDDISIVLNEDMLNLCLSCTKVIDNKDSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQQ8</accession>
    <name>Q3YQQ8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305020.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68947">
        <property type="protein sequence ID" value="AAZ68947"/>
        <property type="gene ID" value="Ecaj_0916"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0916"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="LAINACY"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230951" evidence="1">
        <location>
            <begin position="26"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="278" mass="30486" checksum="697CB6CA413BBA68" modified="2005-09-27" version="1">MNCKKILITTALISLMYSIPSISFSDTIQDGNMGGNFYISGKYVPSVSHFGSFSAKEESKSTVGVFGLKHDWDGSPILKNKHADFTVPNYSFRYENNPFLGFAGAIGYSMGGPRIEFEISYEAFDVKSPNINYQNDAHRYCALSHHTSAAMEADKFVFLKNEGLIDISLAINACYDIINDKVPVSPYICAGIGTDLISMFEATSPKISYQGKLGISYSINPETSVFIGGHFHRIIGNEFRDIPAIVPSNSTTISGPQFATVTLNVCHFGLELGGRFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQR0</accession>
    <name>Q3YQR0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0914</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305018.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0914"/>
    <dbReference type="EnsemblBacteria" id="AAZ68945">
        <property type="protein sequence ID" value="AAZ68945"/>
        <property type="gene ID" value="Ecaj_0914"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0914"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="283" mass="31626" checksum="24A4B237C9C10715" modified="2005-09-27" version="1">MNCKKVFTISALISSIYFLPNVSYSNPVYGNSMYGNFYISGKYMPSVPHFGIFSAEEEKKKTTVVYGLKENWAGDAISSQSPDDNFTIRNYSFKYASNKFLGFAVAIGYSIGSPRIEVEMSYEAFDVKNPGDNYKNGAYRYCALSHQDDADDDMTSATDKFVYLINEGLLNISFMTNICYETASKNIPLSPYICAGIGTDLIHMFETTHPKISYQGKLGLAYFVSAESSVSFGIYFHKIINNKFKNVPAMVPINSDEIVGPQFATVTLNVCYFGLELGCRFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQR2</accession>
    <name>Q3YQR2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0832</name>
        <name evidence="2" type="ordered locus">Ecaj_0912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2005-07" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Ehrlichia canis str. Jake.</title>
            <authorList>
                <person name="Palenik B."/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Pitluck S."/>
                <person name="Chain P."/>
                <person name="Malfatti S."/>
                <person name="Shin M."/>
                <person name="Vergez L."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Mavrommatis K."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304940.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0832"/>
    <dbReference type="EnsemblBacteria" id="AAZ68863">
        <property type="protein sequence ID" value="AAZ68863"/>
        <property type="gene ID" value="Ecaj_0832"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68943">
        <property type="protein sequence ID" value="AAZ68943"/>
        <property type="gene ID" value="Ecaj_0912"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0832"/>
    <dbReference type="KEGG" id="ecn:Ecaj_0912"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="276" mass="30659" checksum="CE51AB37D17AF3A4" modified="2005-09-27" version="1">MNCKKILITTTLVSLTILLPGISFSKPIHENNTTGNFYIIGKYVPSISHFGNFSAKEEKNTTTGIFGLKESWTGGIILDKEHAAFNIPNYSFKYENNPFLGFAGVIGYSIGSPRIEFEVSYETFDVQNPGDKFNNDAHKYCALSNDSSKTMKSGKFVFLKNEGLSDISLMLNVCYDIINKRMPFSPYICAGIGTDLIFMFDAINHKAAYQGKLGFNYPISPEANISMGVHFHKVTNNEFRVPVLLTAGGLAPDNLFAIVKLSICHFGLEFGYRVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQS2</accession>
    <name>Q3YQS2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305009.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0902"/>
    <dbReference type="EnsemblBacteria" id="AAZ68933">
        <property type="protein sequence ID" value="AAZ68933"/>
        <property type="gene ID" value="Ecaj_0902"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0902"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="TVMKNNG"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230984" evidence="1">
        <location>
            <begin position="25"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="278" mass="30771" checksum="28E21F99725BC809" modified="2005-09-27" version="1">MNNKKSLLIGTILLSLFSSLPIKAFSVINHSDISSNVNGLYFTGQYRPAVSHFSGFTVRETNIATQQLVSLNTNKNENHIIEKTNFSGIYTAKFQDNAASFSGAIGYSYPEGLKFEIEISYEKFGVKSTKNYQSTNAVIFALARQATSSNPSDNKYVVMKNSGLSVASVMINGCYNMSFYNLVVSPYICAGIGEDFIEFFDTLYIKLAYQGKLGVNYSLSSRFNIFADMYYHKVIGNQFKNLNVIHAVALDTFPKVTSAIATLNVAYFGGEVGIRFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQS8</accession>
    <name>Q3YQS8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Surface antigen msp4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0896</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011305003.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0896"/>
    <dbReference type="EnsemblBacteria" id="AAZ68927">
        <property type="protein sequence ID" value="AAZ68927"/>
        <property type="gene ID" value="Ecaj_0896"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0896"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078984_0_0_5"/>
    <dbReference type="OMA" id="SPNENEY"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002566">
        <property type="entry name" value="Surface_Ag_msp4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01617">
        <property type="entry name" value="Surface_Ag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="275" mass="31031" checksum="BBE08C890B55404A" modified="2005-09-27" version="1">MNNKLSLLYIALILFTSHVSSALVLNDHNLVYFGIQYKPARHHLSNLLIKESKSDVVEVLALKYDAIGSPLDSTKEVNNFTIKYNPHYDNNRLGFSVIFGYYYNKNFRIESEISHEIFQLKNEGHKRVGFEKYFALKFAPPSSTQGYRHVTLINNGISTTSALINACYDVLIPAHNIITYSCLGFGIDIVDFLSKYTTKFSHQGKLGASYPISHRMSVFTEVYYHGLFGKKFEQLPLNYNANTSPPQQPPHVHTTASAILSIGYYGGSVGIKFIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQT7</accession>
    <name>RECA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein RecA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Recombinase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">recA</name>
        <name type="ordered locus">Ecaj_0887</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304994.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YQT7"/>
    <dbReference type="SMR" id="Q3YQT7"/>
    <dbReference type="STRING" id="269484.Ecaj_0887"/>
    <dbReference type="EnsemblBacteria" id="AAZ68918">
        <property type="protein sequence ID" value="AAZ68918"/>
        <property type="gene ID" value="Ecaj_0887"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0887"/>
    <dbReference type="eggNOG" id="COG0468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040469_3_2_5"/>
    <dbReference type="OMA" id="DYGEQAL"/>
    <dbReference type="OrthoDB" id="1080436at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00983">
        <property type="entry name" value="recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00268">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013765">
        <property type="entry name" value="DNA_recomb/repair_RecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020584">
        <property type="entry name" value="DNA_recomb/repair_RecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023400">
        <property type="entry name" value="RecA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45900">
        <property type="entry name" value="PTHR45900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00154">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00142">
        <property type="entry name" value="RECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54752">
        <property type="entry name" value="SSF54752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02012">
        <property type="entry name" value="tigrfam_recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00321">
        <property type="entry name" value="RECA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_1000047915" description="Protein RecA">
        <location>
            <begin position="1"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="71"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00268"/>
        </source>
    </evidence>
    <sequence length="357" mass="38758" checksum="BCCCF8B43F779F56" modified="2005-09-27" version="1">MSDSKNLNQERQKALDNAISQIEKAFGRGAIMKLKQGAIEKIDSISTGSIALDTALGIGGFPKGRIVEIFGPESSGKTTLALHVIAESQKKGGNCAFIDAEHALDIMYARKLGVNTGDLIVSQPDTGEQALHIVEYLVCSGAIDVIVVDSVAALTPRAEIEGDMGDQHMGLQARLLSHALRKLTSIVSKANCVLIFINQIRMKIGVVYGNPETTTGGNALKFYSSVRLDIRKVSAIKDKDLIIGNQTKVKVVKNKVAPPFKQVDFDIMYNEGISKVGEIIDMGVKLNIIEKAGAYYSYNGIRLGQGKENAKSYLKTNYTTADEIEQKIRNMLASDSDVTCFNTEGSDNLHEVEEAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQT8</accession>
    <name>Q3YQT8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="4">OPRT</shortName>
            <shortName evidence="4">OPRTase</shortName>
            <ecNumber evidence="2 4">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pyrE</name>
        <name evidence="6" type="ordered locus">Ecaj_0886</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 4"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304993.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0886"/>
    <dbReference type="EnsemblBacteria" id="AAZ68917">
        <property type="protein sequence ID" value="AAZ68917"/>
        <property type="gene ID" value="Ecaj_0886"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0886"/>
    <dbReference type="eggNOG" id="COG0461">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074878_3_0_5"/>
    <dbReference type="OMA" id="YFQCAKV"/>
    <dbReference type="OrthoDB" id="1280396at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006273">
        <property type="entry name" value="Orotate_PRibTrfase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01367">
        <property type="entry name" value="pyrE_Therm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="5">
        <location>
            <begin position="43"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-phosphoribose 1-diphosphate binding" evidence="4">
        <location>
            <begin position="121"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="198" mass="21896" checksum="A8F0C2A6911E1FE0" modified="2005-09-27" version="1">MEVDLLNNDAICDEFTRMGVIINGHFVLSSGLHSDTYVQCARVFENPNLAVKYCALLAKKIDRVLPNIDLIVAPAIGAITIGYEIARQLSIINMFCERVNGAFVLRRGFEIQKGSKVLIVEDVITTGKTSMEVVNCVKKNGGTVVAGAALVKRSRDIKLPFPIISLIELNIKSYVDEDIPEYLRQIPVSIPGSRYLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-02-10" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQV3</accession>
    <name>Q3YQV3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycerol-1-phosphate dehydrogenase (NAD(P))</fullName>
            <ecNumber evidence="10">1.1.1.261</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0871</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.261" evidence="10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304979.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0871"/>
    <dbReference type="EnsemblBacteria" id="AAZ68902">
        <property type="protein sequence ID" value="AAZ68902"/>
        <property type="gene ID" value="Ecaj_0871"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0871"/>
    <dbReference type="eggNOG" id="COG0371">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038362_1_0_5"/>
    <dbReference type="OMA" id="DFICRST"/>
    <dbReference type="OrthoDB" id="717704at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050492">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032837">
        <property type="entry name" value="G1PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="PTHR43616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13685">
        <property type="entry name" value="Fe-ADH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="419" mass="47102" checksum="53B854DFAFA86CDE" modified="2005-09-27" version="1">MYNEFLKQVLIDENFCGLESVVNTIKSIHIGRGISNDLCDIVKQYGNNGFIVTDVNIAPLLSKAVLNGFKHLIIPYLASASQKLVELVKEKSQDSDVLVSFGSGTINDICKYVSYVTNKSYISFPTAPSMNGYTSSNASIILNNGCKQSLQSHLPKAIYLDVDIIVNAPERLIVSGFADFICRSTAQADWLLSHLLLGSKYTELPFLISKESENALIVDYLGLIKRDEYSIMVLMQALLLSGLGMFIVGSSHSASQGEHMVAHTIELLQSNMSFFHGEFVGVSTITMACLQHRILKIVPKLYPTLINAQNVKQYFHIQYAEEFCDILDKKFINQQRADYLNNLISDKWSFIVENIKERVLSDLLIKEMLINIGCPNKPEHIGWNASKYSKVIEFAFVTRQRFTFLDIAHHARLPIVEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQV6</accession>
    <name>Q3YQV6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytochrome c oxidase, subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0868</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304976.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0868"/>
    <dbReference type="EnsemblBacteria" id="AAZ68899">
        <property type="protein sequence ID" value="AAZ68899"/>
        <property type="gene ID" value="Ecaj_0868"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0868"/>
    <dbReference type="eggNOG" id="COG0843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044559_0_0_5"/>
    <dbReference type="OMA" id="NHMKYLG"/>
    <dbReference type="OrthoDB" id="1476526at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="400"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="436" mass="48902" checksum="98260DCF0273FB99" modified="2005-09-27" version="1">MLNVNTYNLKLCHKWLVLGVSALGISGLLSIFVILLRLPISKSFILNVDKVFDTSLVIHVNLSVLVWASSIVSIISSLIISTNKYSKCFTYLCYLAFIGTFLMVLSVFFPNAEPIKNNYVPVINNTCFLSGLIIFIVSILFHSILSIKSHNLKIHQDVALGVHGISIILICAVLCFTMSYYSIHKNNYFSIISFYENVFWGGGHILQMAFSQALLVVYLIMLGTNNKLLNKNLINTIFIINTLSTVIGPIVYIYHPSDSQFAIDFFIWHMRILGGIIPVFVFILTLFNLKTLLKHNYHSLICTTLLFSYGGILGILTIHGNVTIPAHYHGSIVGMTIAFMGFIYWLLPKLNFGYTNNFYTNLQVYVYSLGQFLHITGLEWLGGYGALRKVAYLPDTASKIAKHCFTLGGLMAIIGGCMFVVIVLLQIRKKKSNEVQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQV9</accession>
    <name>FABZ_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ</fullName>
            <ecNumber evidence="1">4.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase</fullName>
            <shortName evidence="1">(3R)-hydroxymyristoyl-ACP dehydrase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Beta-hydroxyacyl-ACP dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fabZ</name>
        <name type="ordered locus">Ecaj_0865</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioester dehydratase family. FabZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.59" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304973.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YQV9"/>
    <dbReference type="SMR" id="Q3YQV9"/>
    <dbReference type="STRING" id="269484.Ecaj_0865"/>
    <dbReference type="EnsemblBacteria" id="AAZ68896">
        <property type="protein sequence ID" value="AAZ68896"/>
        <property type="gene ID" value="Ecaj_0865"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0865"/>
    <dbReference type="eggNOG" id="COG0764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078912_1_2_5"/>
    <dbReference type="OMA" id="FPGRPLM"/>
    <dbReference type="OrthoDB" id="1763197at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008659">
        <property type="term" value="F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008693">
        <property type="term" value="F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047451">
        <property type="term" value="F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004317">
        <property type="term" value="F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00406">
        <property type="entry name" value="FabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010084">
        <property type="entry name" value="FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="PTHR30272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01750">
        <property type="entry name" value="fabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000230813" description="3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00406"/>
        </source>
    </evidence>
    <sequence length="148" mass="16629" checksum="09EACAD1A0B17A43" modified="2005-09-27" version="1">MQFNIQEIIKMIPHSYPFLLIDKVIACTPNESTIAVKNVTFNEPFFIGHFPGNPIMPGVLIVEAMAQACMICVISNDQGQNTQDYSVYFMSIELAKFRKPVIPGDTLIIEVNVTHKRNNTCKFQCHAQVENTLVAEAQILAMIKQNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQW9</accession>
    <name>Q3YQW9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptide chain release factor N(5)-glutamine methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.297</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0855</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = H(+) + N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42896"/>
            <dbReference type="Rhea" id="RHEA-COMP:10271"/>
            <dbReference type="Rhea" id="RHEA-COMP:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
            <dbReference type="EC" id="2.1.1.297"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.297" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304963.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0855"/>
    <dbReference type="EnsemblBacteria" id="AAZ68886">
        <property type="protein sequence ID" value="AAZ68886"/>
        <property type="gene ID" value="Ecaj_0855"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0855"/>
    <dbReference type="eggNOG" id="COG2890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_3_1_5"/>
    <dbReference type="OMA" id="IYYGHGT"/>
    <dbReference type="OrthoDB" id="1816476at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:protein-(glutamine-N5) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040758">
        <property type="entry name" value="PrmC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019874">
        <property type="entry name" value="Release_fac_Glu-N5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17827">
        <property type="entry name" value="PrmC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03534">
        <property type="entry name" value="RF_mod_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PrmC_N" evidence="8">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="MTS" evidence="7">
        <location>
            <begin position="117"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="280" mass="32019" checksum="B3D7BF400CAA514E" modified="2005-09-27" version="1">MDTVISLYNSAVNFLSNYNIENPKRDVEIIIKHLIDTKSLVTILDPYMSVDKCDVEMFWKMIKQRAANVPVSHIIGRREFWSTEFIVNADVLDPRPDSETIISSVLAMYPCKNRRLVIGDFGTGSGCLLAVLLLKYRNAVGIAIEKSVKAYRVAYQNFKNHQLSSRVKLRLSSWDNCYDVFDLIVSNPPYIKRSKIAKLQPEVKLYEPMIALNGGPVGLEIYLQIFLVIKRCLKKNGVAILEIGEDQHQIHRIVHKYGLKFCTYHNDLSGKLRCIVVKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQZ4</accession>
    <name>Q3YQZ4_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">N5-carboxyaminoimidazole ribonucleotide synthase</fullName>
            <shortName evidence="3 5">N5-CAIR synthase</shortName>
            <ecNumber evidence="3 5">6.3.4.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 5">5-(carboxyamino)imidazole ribonucleotide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">purK</name>
        <name evidence="7" type="ordered locus">Ecaj_0830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="function">
        <text evidence="5">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 5">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ATP + hydrogencarbonate = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19317"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the PurK/PurT family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.18" evidence="3 5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304938.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0830"/>
    <dbReference type="EnsemblBacteria" id="AAZ68861">
        <property type="protein sequence ID" value="AAZ68861"/>
        <property type="gene ID" value="Ecaj_0830"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0830"/>
    <dbReference type="eggNOG" id="COG0026">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_0_1_5"/>
    <dbReference type="OMA" id="ITFDHEH"/>
    <dbReference type="OrthoDB" id="1165275at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034028">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="114"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="155"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="185"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="270"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="363" mass="40745" checksum="5296634FCF56DE75" modified="2005-09-27" version="1">MLHNSYVSPGSTIGIIGGGQLGKMISIAATNLGYKTHLLTDNQDSPSINVTNNVTILDNYYNKELLLEFASSVDIATLEFENIPSTTIDILSQKTKVYPGKTALHISQNRIREKQYIRNLGIKTANFRIIDNYDNLVHNVLELGYPALLKTTELGYDGKGQYLITQPGDLNTLSTLNWQQEYILEKFVKIHKEISIIISKSINGSIEFFPVAENHHTNGILATSSVPASISEEINIQAKNIALQIAESFNLVGILAVEFFITDEQELIVNEIAPRPHNSGHWSLDACNISQFEQLTRAICGLPLKPVKLLFPCTMDNILGDDIYNYYQYKTKINESIYIYGKTKASKNRKMGHINRLNLKHEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQZ8</accession>
    <name>Q3YQZ8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoribosyl pyrophosphate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0826</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304934.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0826"/>
    <dbReference type="EnsemblBacteria" id="AAZ68857">
        <property type="protein sequence ID" value="AAZ68857"/>
        <property type="gene ID" value="Ecaj_0826"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0826"/>
    <dbReference type="eggNOG" id="COG0462">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_2_0_5"/>
    <dbReference type="OMA" id="FGWARQD"/>
    <dbReference type="OrthoDB" id="1011035at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran_N" evidence="3">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="318" mass="35068" checksum="A23BCFDC25E0C4A3" modified="2005-09-27" version="1">MLISSGTSSINLARCISKTTGIKLINTCITRFSDQELNVEVQENQDDKNKHVIVINSLCFPAHDNLIELLLLIDATNRALCPSKITLIVPYLCYTRQDRVMYRNLDSSSLMVSALSAKVIINTLETANINNIIFIDLHSNQLTGFFDTSITNLSSHAIFIEDIAGKYDMDNLVIVSPDYGALNRTRVFANALSRQYKLNNEIQVAVIDKYRAKPGVSEVMNIAGNVENKDCIIIDDIVDSAGTLCNAASALKDKGALKVSAYVTHGILSGNAIEKVTNSKLDNLTTTNTINHSSFNTPKIQILSIDKFLSNYILYNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR01</accession>
    <name>Q3YR01_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Outer membrane efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YR01"/>
    <dbReference type="STRING" id="269484.Ecaj_0823"/>
    <dbReference type="EnsemblBacteria" id="AAZ68854">
        <property type="protein sequence ID" value="AAZ68854"/>
        <property type="gene ID" value="Ecaj_0823"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0823"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_0_1_5"/>
    <dbReference type="OMA" id="YRKGDND"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="432" mass="48030" checksum="02302F4C68BCF485" modified="2005-09-27" version="1">MIVGSILMIIKFNMHKIYSILILIFIISITPNSSYCTNLDQALHTALSNNPNIKAKLYNYLGNKQKIKLNSISKFLPSVTYLAETYQPNLSLGNNNRTMRLIVTQQVFNGGADAAALQQSKYLSNIEEFEFLSEQQNIVLNTVKAYMKVLTTAEVYKLTQHTKKVFTEHLTATQKRFSLGEVTKTDVSLATARLSSATSESIKAYGDMKAAEANYIHIVGETPVDLQHPIIPEIPSSVEEALTIAQQNNLSLKASYNGYQAAKQGALMAIANLLPSISISSINSYTYTDTHNPLIEPKKIDHFFEIKMSLPIFQQGLNIAAISQSKLAIKQKMYSHYEVLNAIKEAVVLNWENISTANSMLQAAQDSVKYSEVVLSGIKQEAELNLRTVLDVLDAEQELLKAKVNLVNVQSNVVISMYNLLALIGQLNINYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR05</accession>
    <name>Q3YR05_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="5">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="5">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ppa</name>
        <name evidence="6" type="ordered locus">Ecaj_0819</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphosphate + H2O = H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304927.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0819"/>
    <dbReference type="EnsemblBacteria" id="AAZ68850">
        <property type="protein sequence ID" value="AAZ68850"/>
        <property type="gene ID" value="Ecaj_0819"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0819"/>
    <dbReference type="eggNOG" id="COG0221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073198_1_0_5"/>
    <dbReference type="OMA" id="DEPTFPG"/>
    <dbReference type="OrthoDB" id="1767807at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="PTHR10286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="SSF50324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="172" mass="19306" checksum="7968E5F170FDF56A" modified="2005-09-27" version="1">MNLDNLTSGDNVPKEINVVIEISQNSCPVKYEFDKKKNLFCVDRFLPTSMYYPCNYGFIPHTCAGDGDPVDVLVTTRFPVMPGAVLCARPVGVLVMCDEGGEDVKILAVPTSKIDQHYANIKNYSDFPVSFLNSITHFFSFYKKLEEDKFVSVDGWRDIKAAEDFILSALTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-04-07" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR09</accession>
    <name>Q3YR09_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Beta sliding clamp</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ecaj_0815</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a ring-shaped head-to-tail homodimer around DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the beta sliding clamp family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304923.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0815"/>
    <dbReference type="EnsemblBacteria" id="AAZ68846">
        <property type="protein sequence ID" value="AAZ68846"/>
        <property type="gene ID" value="Ecaj_0815"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0815"/>
    <dbReference type="eggNOG" id="COG0592">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038149_4_2_5"/>
    <dbReference type="OMA" id="YLIMPVR"/>
    <dbReference type="OrthoDB" id="1040142at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00140">
        <property type="entry name" value="beta_clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001001">
        <property type="entry name" value="DNA_polIII_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022635">
        <property type="entry name" value="DNA_polIII_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022637">
        <property type="entry name" value="DNA_polIII_beta_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022634">
        <property type="entry name" value="DNA_polIII_beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30478">
        <property type="entry name" value="PTHR30478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00712">
        <property type="entry name" value="DNA_pol3_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02767">
        <property type="entry name" value="DNA_pol3_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02768">
        <property type="entry name" value="DNA_pol3_beta_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000804">
        <property type="entry name" value="DNA_pol_III_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00480">
        <property type="entry name" value="POL3Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00663">
        <property type="entry name" value="dnan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 10" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6 10 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol3_beta" evidence="11">
        <location>
            <begin position="8"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_2" evidence="12">
        <location>
            <begin position="136"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_3" evidence="13">
        <location>
            <begin position="256"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAZ68846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="375" mass="41971" checksum="C3D3E4E52B8B6297" modified="2005-09-27" version="1">MNGGIKLNFFVSKDKLLQALSYINSVVEKRHSIVVLSCVRIEAKDHQINLTSTDLDISIIASISAHINENGVVVVSAQLLYDIVKKLPNDIEIKFVIGKDGRLVIECGRTEFFLPVVDIEKFPIIENNELQNKFTLQVSELVNLFGKTKFAISVEESRFNLNGVYVHTADNILCCAATDGHRLSLAKLSKSQNMLSNFGIIVPKKTVNEILKIVSHGSNNDLISVSLSDRKICFEYQEYILISKVIDGTFPDYNAIIPKSHNKSVLIESEVLSNAIDRVSVVVFDKVKVIKLRFVDNKLILSAASSDQGDAREELEVDYSGEDMTIGLNARYLMEALYCVKGKCILSFYDAGTSVLMQEQENIDDFTYIIMPMRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR15</accession>
    <name>Q3YR15_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome c oxidase subunit 2</fullName>
            <ecNumber evidence="15">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Ecaj_0809</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 15">Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-[cytochrome c] + 4 H(+)(out) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="15">Binds a copper A center.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="15"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0809"/>
    <dbReference type="EnsemblBacteria" id="AAZ68840">
        <property type="protein sequence ID" value="AAZ68840"/>
        <property type="gene ID" value="Ecaj_0809"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0809"/>
    <dbReference type="eggNOG" id="COG1622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036876_2_3_5"/>
    <dbReference type="OMA" id="HAFMPIA"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd13912">
        <property type="entry name" value="CcO_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034210">
        <property type="entry name" value="CcO_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001505">
        <property type="entry name" value="Copper_CuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014222">
        <property type="entry name" value="Cyt_c_oxidase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011759">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="PTHR22888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02790">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="SSF81464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02866">
        <property type="entry name" value="CoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00078">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50999">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0186">Copper</keyword>
    <keyword evidence="8 14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_TM" evidence="18">
        <location>
            <begin position="10"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_CUA" evidence="17">
        <location>
            <begin position="109"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AAZ68840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="247" mass="28361" checksum="194F9D056E2FB145" modified="2005-09-27" version="1">MFSSFSAFAAPVKWQMGFQTPATEIMESIIKSHNFVMLVMSIVVVVVFSITLYVLIKFRKKPEDKIVFNTKNSHNTVLEILWTLIPLIIVGFLTISNVKLIRHEQQIPKVEMTLKAIGYQWYWGYSYPDYKNFAFDSYMKATDSLEDNDLRFLEVDNRVVVPINTNILLQVTSADVIHSWAVPALGVKIDAVPGRLNEAWFNIKTPGVYYGQCSELCGRLHGFMPIVIVAVEKEQFDEWIQKKTLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR18</accession>
    <name>Q3YR18_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE</fullName>
            <ecNumber evidence="6">2.1.1.163</ecNumber>
            <ecNumber evidence="6">2.1.1.201</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">2-methoxy-6-polyprenyl-1,4-benzoquinol methylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Demethylmenaquinone methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ubiE</name>
        <name evidence="7" type="ordered locus">Ecaj_0806</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9539"/>
            <dbReference type="Rhea" id="RHEA-COMP:9563"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18151"/>
            <dbReference type="ChEBI" id="CHEBI:55437"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.163"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:28286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="EC" id="2.1.1.201"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Quinol/quinone metabolism; menaquinone biosynthesis; menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.163" evidence="6"/>
    <dbReference type="EC" id="2.1.1.201" evidence="6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304914.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0806"/>
    <dbReference type="EnsemblBacteria" id="AAZ68837">
        <property type="protein sequence ID" value="AAZ68837"/>
        <property type="gene ID" value="Ecaj_0806"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0806"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_0_1_5"/>
    <dbReference type="OMA" id="VRNFENL"/>
    <dbReference type="OrthoDB" id="1431378at2"/>
    <dbReference type="UniPathway" id="UPA00079">
        <property type="reaction ID" value="UER00169"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043333">
        <property type="term" value="F:2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043770">
        <property type="term" value="F:demethylmenaquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102094">
        <property type="term" value="F:S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102955">
        <property type="term" value="F:S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102027">
        <property type="term" value="F:S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="231" mass="26382" checksum="8FFAC558EC0D9714" modified="2005-09-27" version="1">MEERSKFVNQVFTSVASRYDLMNDIMSFGLHRLWKNKAIQGITSGRLLDVACGTGDIAIKVTKKTQNNVDIIVCDINPKMLRHGRDNAINANALNLKWVCGNAEQLPFSDNSFDYYTISFGIRNVSNRQLALNEAYRVLKQGGKFICLEFSPLKESHPLHKFYNFYSFSIIPNIGQLVAKDKASYQYLVDSISEFPYQEDFAQEIQNSNFVEIKYNNLCFGIVTLYTALKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR20</accession>
    <name>HSLV_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent protease subunit HslV</fullName>
            <ecNumber evidence="1">3.4.25.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hslV</name>
        <name type="ordered locus">Ecaj_0804</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent cleavage of peptide bonds with broad specificity.</text>
            <dbReference type="EC" id="3.4.25.2"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by HslU binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1B family. HslV subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304912.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YR20"/>
    <dbReference type="SMR" id="Q3YR20"/>
    <dbReference type="STRING" id="269484.Ecaj_0804"/>
    <dbReference type="MEROPS" id="T01.006"/>
    <dbReference type="EnsemblBacteria" id="AAZ68835">
        <property type="protein sequence ID" value="AAZ68835"/>
        <property type="gene ID" value="Ecaj_0804"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0804"/>
    <dbReference type="eggNOG" id="COG5405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093872_1_0_5"/>
    <dbReference type="OMA" id="IMKGNAR"/>
    <dbReference type="OrthoDB" id="1129370at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009376">
        <property type="term" value="C:HslUV protease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01913">
        <property type="entry name" value="protease_HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00248">
        <property type="entry name" value="HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022281">
        <property type="entry name" value="ATP-dep_Prtase_HsIV_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="PTHR32194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039093">
        <property type="entry name" value="HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03692">
        <property type="entry name" value="ATP_dep_HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0915">Sodium</keyword>
    <keyword id="KW-0888">Threonine protease</keyword>
    <feature type="chain" id="PRO_1000012608" description="ATP-dependent protease subunit HslV">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00248"/>
        </source>
    </evidence>
    <sequence length="189" mass="20759" checksum="7F049B3B9E67A95F" modified="2005-09-27" version="1">MEHKDPSQMYGTTILCIRRGNQVIIAGDGQVSLGQTVIKNSAKKIKRLANDTVITGFAGATADAFTLFERLESKLEKHPGQLLRACVELAKDWRMDKYLRRLEAMMIVADKSISLIISGNGDVLEPENGIAAIGSGGNYALAAAKALCETNERFSQNMTLEYTITTAMRIASEICIYTNNNIIIEKIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR22</accession>
    <name>Q3YR22_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0802</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68833">
        <property type="protein sequence ID" value="AAZ68833"/>
        <property type="gene ID" value="Ecaj_0802"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0802"/>
    <dbReference type="HOGENOM" id="CLU_2287061_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="101" mass="12168" checksum="DD545331C0F89A01" modified="2005-09-27" version="1">MHQGNKHSREIHTLLVTYNPKQVNNKTLYLTTTHKTHHPLTYKNIKVEQFHLLPHHYSEVSVYKFINKANSIYKFIIYKHSTHTNILNKFLVSYLFFNNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-08-12" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR30</accession>
    <name>Q3YR30_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP synthase F1 subcomplex epsilon subunit</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0794"/>
    <dbReference type="EnsemblBacteria" id="AAZ68825">
        <property type="protein sequence ID" value="AAZ68825"/>
        <property type="gene ID" value="Ecaj_0794"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0794"/>
    <dbReference type="eggNOG" id="COG0760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058598_0_0_5"/>
    <dbReference type="OMA" id="LWNKILM"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230964" evidence="1">
        <location>
            <begin position="27"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="399" mass="45140" checksum="94AEA913CA0ECBB0" modified="2005-09-27" version="1">MIINMFNHAVRLLILCVMLFSSTVFADVKIVAMVNDDLISNLDLEKRVAINKFFYKVDGSTAAEIALDALIDESIWRQEAKKLKITVTERDILEAVKQFLVMKNLGNIDFKSYVETQGLDYKMFMQHMKSKLLWNKILMLKVIPYIIISEKEIRDNRDCTVTNGIDTSVHIQEIIVPASVSDNESIIDSIMSDLQNGVSVESIQANKKDVLVEEASINVKNIDVDLANKLLNVKVGDMIGPIKNQHGSFIIKLLHRADINREFASSSVNLKQIHLNVEEGKKYSSQISQLKTKATCENFNNIAKEFGLPEPFSFVMKVKDLSVKMQNMLQSCDVGKIVEVTDNNVVDVIMLCSVTKGKADDVIDNADFIKQRLYMEKLAMQSEYLLSSLKKNALIEKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR55</accession>
    <name>Q3YR55_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304877.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0769"/>
    <dbReference type="EnsemblBacteria" id="AAZ68800">
        <property type="protein sequence ID" value="AAZ68800"/>
        <property type="gene ID" value="Ecaj_0769"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0769"/>
    <dbReference type="HOGENOM" id="CLU_2878705_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="63" mass="6856" checksum="B800D5E533ECD0CE" modified="2005-09-27" version="1">MSGDGLTVLNICLLVMIALGVLVAVVLELYSVFADKSGQCADNRRQFEEPKSALYFLWIGGYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR71</accession>
    <name>Q3YR71_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304861.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68784">
        <property type="protein sequence ID" value="AAZ68784"/>
        <property type="gene ID" value="Ecaj_0752"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0752"/>
    <dbReference type="HOGENOM" id="CLU_1025775_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="273" mass="32056" checksum="52FDD3CF70DAB73C" modified="2005-09-27" version="1">MILIDDKIFAMYSKVVVISLVAIVLTLLFVIAVYKFFQMFFFDRQLKKAYDFVHQEVKEGMNKKLIGIHSTGDRCLYKITNTINYIMEQFEALDKCVAIEGNQEKFYRGMIVARMIGAYRSREQLSEYEKSLLRKLKSEYAEDLPIDIRAEAFWYIRDFVNGVCADIRDKSFVHFKLKPELFILGRMKVALEILENCMRSLRKDITLEHFFYIMRELSDEQLIETVTSKDPQFMLMLSQSVKEKEDVSNNRISETCFTSSDIIGFNENYVKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR85</accession>
    <name>Q3YR85_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304847.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68770">
        <property type="protein sequence ID" value="AAZ68770"/>
        <property type="gene ID" value="Ecaj_0738"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0738"/>
    <dbReference type="HOGENOM" id="CLU_1822336_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="141" mass="16119" checksum="6AFC96687B651B7B" modified="2005-09-27" version="1">MITGGIGLSNLELTLIICLVLLLLALLAFIINKCCAKPKYEPLPSDKQQDYKTLQGDYDELKKVYAKLEQEHEQVKCNLNDLKKVYSDAAGLLSKLESDLGLLRSDYNMLQNQYEGLEKDRLEALHAFRSSMKHYAMLYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR91</accession>
    <name>Q3YR91_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68764">
        <property type="protein sequence ID" value="AAZ68764"/>
        <property type="gene ID" value="Ecaj_0732"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0732"/>
    <dbReference type="HOGENOM" id="CLU_2129490_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="113" mass="13144" checksum="25200437CD9318A8" modified="2005-09-27" version="1">MVMNDYDSVILGFFILFIVLTIIMFCTCCVFCTRSVLYDRLIESEIKKYKDVEEQESNSLGWTGIFVNRMRDTKWSINKDGIRSTLIRQLELQGPLRNQVINPDGNRNDIGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRA1</accession>
    <name>Q3YRA1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0722</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304831.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68754">
        <property type="protein sequence ID" value="AAZ68754"/>
        <property type="gene ID" value="Ecaj_0722"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0722"/>
    <dbReference type="HOGENOM" id="CLU_2751394_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="70" mass="7683" checksum="3ECCCD247E5F97AC" modified="2005-09-27" version="1">MILYFSDGIGNQRAIQRNAGKKNNGKVEDSEKSSTLIESEGQENTEVKCRSTTTNLKDIQSSSCSGEQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRB3</accession>
    <name>Q3YRB3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DSBA oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0710</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be required for disulfide bond formation in some proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thioredoxin family. DsbA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YRB3"/>
    <dbReference type="STRING" id="269484.Ecaj_0710"/>
    <dbReference type="EnsemblBacteria" id="AAZ68742">
        <property type="protein sequence ID" value="AAZ68742"/>
        <property type="gene ID" value="Ecaj_0710"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0710"/>
    <dbReference type="eggNOG" id="COG1651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_47_4_5"/>
    <dbReference type="OMA" id="YREWPIL"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="51"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="246" mass="27734" checksum="BFF09774869CF20C" modified="2005-09-27" version="1">MLRILFLLSLVILVASFPLINNWLSNKSGKPIVDKDTIIAIIEEYISNYPQKVIDLLTKGQVRAENEEMSQNIKKYKSELENTSYPSAGNKDSKIVFVEFFDYSCGYCKMMSEDMKQIVQDGKVHVIFRDFPILGESSLKVAQAALAVHMINPNKYIDFYYAALHYKQQFNDESILSIIKSIGITEEDFKVSLAKNADAIDKMIQSTRELAQNINIRGTPAIIVGDTFIGGAADISTLRSKIDMQK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRC1</accession>
    <name>DNLJ_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polydeoxyribonucleotide synthase [NAD(+)]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ligA</name>
        <name type="ordered locus">Ecaj_0702</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.</text>
            <dbReference type="EC" id="6.5.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD-dependent DNA ligase family. LigA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRC1"/>
    <dbReference type="SMR" id="Q3YRC1"/>
    <dbReference type="STRING" id="269484.Ecaj_0702"/>
    <dbReference type="EnsemblBacteria" id="AAZ68734">
        <property type="protein sequence ID" value="AAZ68734"/>
        <property type="gene ID" value="Ecaj_0702"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0702"/>
    <dbReference type="eggNOG" id="COG0272">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007764_2_1_5"/>
    <dbReference type="OMA" id="HDVEHEI"/>
    <dbReference type="OrthoDB" id="241401at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003911">
        <property type="term" value="F:DNA ligase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00114">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01588">
        <property type="entry name" value="DNA_ligase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001679">
        <property type="entry name" value="DNAligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013839">
        <property type="entry name" value="DNAligase_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013840">
        <property type="entry name" value="DNAligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004150">
        <property type="entry name" value="NAD_DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004149">
        <property type="entry name" value="Znf_DNAligase_C4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01653">
        <property type="entry name" value="DNA_ligase_aden"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03120">
        <property type="entry name" value="DNA_ligase_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03119">
        <property type="entry name" value="DNA_ligase_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001604">
        <property type="entry name" value="LigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00532">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="SSF52113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00575">
        <property type="entry name" value="dnlj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000313223" description="DNA ligase">
        <location>
            <begin position="1"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="594"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="1">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01588"/>
        </source>
    </evidence>
    <sequence length="677" mass="76854" checksum="A54363B8D5F86757" modified="2005-09-27" version="1">MKQEEAKLELDKLNSQIQHHDFLYYTQDNPQITDAEYDVLCHKRNLILESFPELASNNNYQDNVGSTPDAKFAKVKHAEKMLSLDNAFNQQDIEKFITRTKKLLDMDNSQSIAISCELKIDGLSFSVIYKKGEISQASTRGNGYFGENITNNVKTIKNLPHTIQNAPDSLEVRGEIYIDRSDFIQLNKDGNNFANPRNAAAGSVRQLDINITAQRKLKYFMYTIVNTKCLTQEETLNQLKTLGFCVNEHTITTNNIEDALNFYNQFYNNRSNISYDIDGIIYKVNDIKSQHILGATNKSPRWAIAYKFPAAEAKTLVNKISIQIGRTGVLTPIAELSPINIGGVIVTRASLHNKSEIERKDIREGDYVIVKRAGDVIPQVVDVDKNLRAQELTKFIFPTTCPSCGSNLFQAEQEVSIYCTGELFCKNQILEKIKHFVSKDAFNIIGFGKKQLLFFYEQGLITNIVDIFTLEEKISNSDLKLESLHGWGEKSIHNLFSAINNSKTISLENFIFALGIRFVGKYIAKILAKHFLSYEKWYHEMLRLAQDENYLLNIQQIGHKTIHSLKMFFTEQHNLDTINNLVRHLTITDAKTTSHLSLLHGKTIVFTGELSNMSRHEAKTKSETAGAKVSSSLSKNTDFLIVGNNPGSKYKKAQSLNIQILTEDLWLQYISPNTNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRD3</accession>
    <name>Q3YRD3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ABC transporter, transmembrane region:ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0690</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of an ABC transporter complex. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304799.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0690"/>
    <dbReference type="EnsemblBacteria" id="AAZ68722">
        <property type="protein sequence ID" value="AAZ68722"/>
        <property type="gene ID" value="Ecaj_0690"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0690"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_4_5"/>
    <dbReference type="OMA" id="RVMHDLR"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="20"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="332"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="367"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="581" mass="64353" checksum="3C0A679B7C5E1A14" modified="2005-09-27" version="1">MKALFQFVKPHLLYFIFAYLVVAVSSVTILAFGYGLHNLVDSWVATSDVSSINNSLLFLLAIVLIVALTSFLRLWLTGYGSERVILGIRLKLYNKIIHFSPRFFENNSISSLITRLMADTSTLQVILSASMSTILRNVTTLLGSIVMLIHTNFKLTMYTIVVIPVILLILTFLGKKVKLLSGKVRLGIDNITSFVEETYRGVYVIQSFSKEDLTKQKFENLLANVLNITNRYILLRALLVTLIITSAMISIGFVLFLGIREVLNSNITVGSLFSFIFYAAIAAGSVNNMGDNFNDIQKAIRIAEDISKILSMSSDIVEVAHPQEIKEIKNRISINDVTFNYPSKPDTPALKNVSFTIDRGQVVALVGPSGSGKSTIVNILLRFYDVSTGSITIDGIDIKELSIKNLRSLFSIIPQSPIMFSGTIWDNITYGVADATYEEVRSAAETACILDFIEGLPEKFDAFVGERGLCLSEGQKQRIVIARAILKDSGVLILDEATSSLDSKNEQLIHDALDNLRSNKTMLVIAHRLSTIIKADKIIVFNNGCIDDIGTHKSLISKQDGLYTKLAKLQLSSDYFSDNHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRD8</accession>
    <name>Q3YRD8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bcr/CflA family efflux transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0685</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Bcr/CmlA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0685"/>
    <dbReference type="EnsemblBacteria" id="AAZ68717">
        <property type="protein sequence ID" value="AAZ68717"/>
        <property type="gene ID" value="Ecaj_0685"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0685"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_47_1_5"/>
    <dbReference type="OMA" id="YFFMLNI"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004812">
        <property type="entry name" value="Efflux_drug-R_Bcr/CmlA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00710">
        <property type="entry name" value="efflux_Bcr_CflA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="1"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="386" mass="42384" checksum="4F0E04581CE4A913" modified="2005-09-27" version="1">MCDMSSDIYLPALPKISEFFNVDHTIVQLTVSLNLVGISVSGLLYGPLSDYYGRRPIILLGVGIFMVASIMCCFSSNIIMLIIMRFIQGFGAGVAGVVGYAIIKDMYSGNECAKNISIVNIAVAFAPVVAPILGSAIIAHGYHWNMLFVTISIMAVLVLASLFMFLQETISFDNVDSSISFLSIIRKYKELVLNIRFCGFALIQSFTIMWIWSCVANLPFIFVNDMGVPVGYYGYFVAINVAAYIVGAIINQRFVERFGINNMLLIGLILTTLSDLVVLVLYQIIEISPLLAEIMWMPSGMGIAFILGNNMTAAFSEIKEPGIGSAFILFLQTIFGALGIYILGCFYDGTLIPVILFPIICSVICLVIYAFLQVTNKSNRSKFVDV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRE2</accession>
    <name>KGUA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Guanylate kinase</fullName>
            <ecNumber evidence="1">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gmk</name>
        <name type="ordered locus">Ecaj_0681</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for recycling GMP and indirectly, cGMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + GMP = ADP + GDP</text>
            <dbReference type="Rhea" id="RHEA:20780"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304790.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRE2"/>
    <dbReference type="SMR" id="Q3YRE2"/>
    <dbReference type="STRING" id="269484.Ecaj_0681"/>
    <dbReference type="EnsemblBacteria" id="AAZ68713">
        <property type="protein sequence ID" value="AAZ68713"/>
        <property type="gene ID" value="Ecaj_0681"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0681"/>
    <dbReference type="eggNOG" id="COG0194">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001715_1_2_5"/>
    <dbReference type="OMA" id="EWAVVHG"/>
    <dbReference type="OrthoDB" id="1502854at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:guanylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000266319" description="Guanylate kinase">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="1">
        <location>
            <begin position="9"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00328"/>
        </source>
    </evidence>
    <sequence length="209" mass="24250" checksum="899E8AEC9684808B" modified="2005-09-27" version="1">MNDNLKSSGIMLVISSPSGGGKTTISHLLIDELQNDLVRSVSVTTREPRDGEVNGKDYFFVTEHEFINLCNTDQMLEYAKVFGNYYGIPRKFVMDNITTGVSVLFSIDWQGAFKLIDIMREHVVSVFILPPSMEELRRRLYNRSGKSDIVKKRLGEAAFEIDHCYRYDYVIVNHDVEESVHQIKCIFTSERLRVQRRILLKQFIDNYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRF2</accession>
    <name>Q3YRF2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transport-associated domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0671"/>
    <dbReference type="EnsemblBacteria" id="AAZ68703">
        <property type="protein sequence ID" value="AAZ68703"/>
        <property type="gene ID" value="Ecaj_0671"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0671"/>
    <dbReference type="eggNOG" id="COG2823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083606_5_2_5"/>
    <dbReference type="OMA" id="RVKKVHN"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007055">
        <property type="entry name" value="BON_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014004">
        <property type="entry name" value="Transpt-assoc_nodulatn_dom_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04972">
        <property type="entry name" value="BON"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00749">
        <property type="entry name" value="BON"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50914">
        <property type="entry name" value="BON"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BON" evidence="1">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="BON" evidence="1">
        <location>
            <begin position="110"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="183" mass="20153" checksum="A9DC768ED082CC2B" modified="2005-09-27" version="1">MINQTGCSGIMAGLAVTTGAIITLQDRSIGDTIDDTAILIRINKELFKVGLFPQVTVKVSEGRVLLLGHVKSLDDQITAEKVSWQQKDVREVVNEITVDKGSINLKETAIDAAISAQVKARILSQSKVKSMNYSVNTIDRIVYLMGIAQDQEELNKVVSIAQKVKKVKQVVSYVRLKDSKLRN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRF6</accession>
    <name>GPDA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-3-phosphate dehydrogenase [NAD(P)+]</fullName>
            <ecNumber evidence="1">1.1.1.94</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NAD(P)H-dependent glycerol-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gpsA</name>
        <name type="ordered locus">Ecaj_0667</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:11096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.94" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304776.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRF6"/>
    <dbReference type="SMR" id="Q3YRF6"/>
    <dbReference type="STRING" id="269484.Ecaj_0667"/>
    <dbReference type="EnsemblBacteria" id="AAZ68699">
        <property type="protein sequence ID" value="AAZ68699"/>
        <property type="gene ID" value="Ecaj_0667"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0667"/>
    <dbReference type="eggNOG" id="COG0240">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033449_0_0_5"/>
    <dbReference type="OMA" id="NRMFGNM"/>
    <dbReference type="OrthoDB" id="1419877at2"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009331">
        <property type="term" value="C:glycerol-3-phosphate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004367">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase [NAD+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00394">
        <property type="entry name" value="NAD_Glyc3P_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000255308" description="Glycerol-3-phosphate dehydrogenase [NAD(P)+]">
        <location>
            <begin position="1"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="255"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00394"/>
        </source>
    </evidence>
    <sequence length="324" mass="35038" checksum="AA97E5D54BA2354F" modified="2005-09-27" version="1">MKITILGAGSFGTAIAIALSSISTSINLWGRNHTDMQSITINRKNLKYLPTYELPDNIIPSSSIDEVLSDHNTCIILAVPTQQLRTLCLYVQQHIFEQTPLLICSKGIENISLKFPSEIVKEILPNNPTYILSGPSFAKEIAANLPCTIVLAGENEVLGTSLINKMSNKTFKIVYSKDTLGVQIGAALKNIIAIACGIITGKNLGNNAVASVIAKGMEEIKTLYTAKNETINLSTLIGPSCLGDLILTCTTTHSRNMSFGVAIGQGEDINKMLNKKSEIIEGVSTVKPLISLAEKLNIELPICTSIYNLLYKNVSLDKTISEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRH1</accession>
    <name>Q3YRH1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0652</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304761.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0652"/>
    <dbReference type="EnsemblBacteria" id="AAZ68684">
        <property type="protein sequence ID" value="AAZ68684"/>
        <property type="gene ID" value="Ecaj_0652"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0652"/>
    <dbReference type="eggNOG" id="COG0047">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001031_3_0_5"/>
    <dbReference type="OMA" id="LHFVCRD"/>
    <dbReference type="OrthoDB" id="1527083at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="264" mass="29643" checksum="BB2FEA2EA77AFAA3" modified="2005-09-27" version="1">MKVVVLSGYGLNCEEETLFAFIKAGELLSCDVQGKIVHINDMILNTKLLVDYNVLVIPGGFSYGDDTGAGNAFALRVFNNLKEEISEFLVGDKLVIGICNGCQILMRLISDFSNITLLNNTVRQYQCRWVKVKVNPSNNSVWLKGLDELYIPVAHGEGRFFVENDSVITSIRNNIALRYITNIGNYANQEFPYNPNGSVDDIAALSDSSGRVLVMMPHPERAMFFTQQDNWTQVKEQCLRDNIAYPTYGDGMKVFCNAVEYFCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRH2</accession>
    <name>Q3YRH2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA repair protein RadC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0649</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the UPF0758 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304760.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0649"/>
    <dbReference type="EnsemblBacteria" id="AAZ68683">
        <property type="protein sequence ID" value="AAZ68683"/>
        <property type="gene ID" value="Ecaj_0649"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0649"/>
    <dbReference type="eggNOG" id="COG2003">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073529_0_0_5"/>
    <dbReference type="OMA" id="HEEFWII"/>
    <dbReference type="OrthoDB" id="1833204at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08071">
        <property type="entry name" value="MPN_DUF2466"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025657">
        <property type="entry name" value="RadC_JAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001405">
        <property type="entry name" value="UPF0758"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020891">
        <property type="entry name" value="UPF0758_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30471">
        <property type="entry name" value="PTHR30471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04002">
        <property type="entry name" value="RadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00608">
        <property type="entry name" value="radc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01302">
        <property type="entry name" value="UPF0758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="7">
        <location>
            <begin position="108"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="230" mass="26122" checksum="15112B17A5FB34DE" modified="2005-09-27" version="1">MNTKDEHKKQQIGHRKRLRQKLLSGGNKGLLDYEILELILCSSHIRKDVKPIAKRLIKHFGSFSKVFFADFNQLTEVHGIGEASVSAILCIRETLNRILREDIDHGVIINQWQKLIEYLRIKIGNGNIENFHILYLNTKYKLLADETQDVGTVNQTPLYIREVIKKSLSLGATSIIIAHNHPSGDARPSKADIEITNQLAITCNNIGITLIDHVIVTSNDHYSFKTHNLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRI2</accession>
    <name>PYRC_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroorotase</fullName>
            <shortName evidence="1">DHOase</shortName>
            <ecNumber evidence="1">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrC</name>
        <name type="ordered locus">Ecaj_0639</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304750.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRI2"/>
    <dbReference type="SMR" id="Q3YRI2"/>
    <dbReference type="STRING" id="269484.Ecaj_0639"/>
    <dbReference type="EnsemblBacteria" id="AAZ68673">
        <property type="protein sequence ID" value="AAZ68673"/>
        <property type="gene ID" value="Ecaj_0639"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0639"/>
    <dbReference type="eggNOG" id="COG0044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_0_5"/>
    <dbReference type="OMA" id="QHAQEPR"/>
    <dbReference type="OrthoDB" id="1319925at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01317">
        <property type="entry name" value="DHOase_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00220_B">
        <property type="entry name" value="PyrC_classI_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000325592" description="Dihydroorotase">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="343"/>
            <end position="344"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00220"/>
        </source>
    </evidence>
    <sequence length="447" mass="49115" checksum="9B695F6620AEBE43" modified="2005-09-27" version="1">MYKQSWKLLGRGQDADFTVAYINARIIDPESKLDIIGSLLTKGDKIVDFGQDLFNNGIPSTIDEVVDCNNNILMPGLIDIHVHFREPGQEHKETIHTGSKSAAAGGVTTVVCQPNTIPTISSIITAKYIKMRALESAYVNIEFYASITKPDNSLSDMALLKEAGAVGFTDDGMPVMNSLTMRQALSYSSMLDTVVAQHAEDLNLSNNGHINEGTVSYELGLKGIPDISESIIVNRDIALMKNIKNVHYHILHVSSQDSLNIIKQAKNQGLKVTCEVTPHHLTLTEQDVITHGTLAKINPPLRTENDRLSMVEGLKNGIIDCIATDHAPHEVNTKELPLDTAAFGIVGLETMLPISLELYHNGTISLIDLLATLTYKPANIIKVPRGRIKKGFVADLIILDLNHEWTIDILKFASKSKNSPFHNRKVKGKVLRTIVSGKTTYTAVRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRK6</accession>
    <name>Q3YRK6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA methyl transferase TrmH, group 3</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0615</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304726.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0615"/>
    <dbReference type="EnsemblBacteria" id="AAZ68649">
        <property type="protein sequence ID" value="AAZ68649"/>
        <property type="gene ID" value="Ecaj_0615"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0615"/>
    <dbReference type="eggNOG" id="COG0566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021322_0_2_5"/>
    <dbReference type="OMA" id="QVPPYEY"/>
    <dbReference type="OrthoDB" id="1422015at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46429">
        <property type="entry name" value="PTHR46429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_sub_bind" evidence="4">
        <location>
            <begin position="7"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="252" mass="28261" checksum="51FBB1348033C7D8" modified="2005-09-27" version="1">MNKNKIWIYGKHACIAALQNKNRECYEMLVTENITKNNSEITKISELCKQRNINLKTVNLNYINSVLPHNSNHQGIALKASTINPLTIEEILNSSTINSTIILLDQITDIHNVGAIMRSAVCFNVDAIILPYHNSPSENCGMSKTSSGAIDMIPIAYVINLVSTIKLFKNAGYWCYGFDTKEGELLHKTKFDNKKLIIFGSEDKGMRKLTKENCDFLLKIATSNKIDSLNVSNAAAVALYSIFIQNYDQTFQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRM2</accession>
    <name>RS14_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsN</name>
        <name type="ordered locus">Ecaj_0599</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S3 and S10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304711.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRM2"/>
    <dbReference type="SMR" id="Q3YRM2"/>
    <dbReference type="STRING" id="269484.Ecaj_0599"/>
    <dbReference type="EnsemblBacteria" id="AAZ68633">
        <property type="protein sequence ID" value="AAZ68633"/>
        <property type="gene ID" value="Ecaj_0599"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0599"/>
    <dbReference type="eggNOG" id="COG0199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139869_0_1_5"/>
    <dbReference type="OMA" id="FGLCRNQ"/>
    <dbReference type="OrthoDB" id="2032609at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00537">
        <property type="entry name" value="Ribosomal_S14_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_S14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023036">
        <property type="entry name" value="Ribosomal_S14_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_S14_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="PTHR19836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000128395" description="30S ribosomal protein S14">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00537"/>
        </source>
    </evidence>
    <sequence length="101" mass="11734" checksum="402FF72D9F3173F9" modified="2005-09-27" version="1">MSRKSVIQRNLKRISICSRLKSKRDELKAIIKDQSISMNDRFLAQVKLSKLPRDSSYIRIRNRCLVTGRPRGCYRKFKVSRIVLRQLGSIGQIPGLTKSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRP0</accession>
    <name>Q3YRP0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4-hydroxybenzoate octaprenyltransferase</fullName>
            <ecNumber evidence="8">2.5.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">4-HB polyprenyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ubiA</name>
        <name evidence="9" type="ordered locus">Ecaj_0581</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of ubiquinone-8 (UQ-8) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate 3-octaprenyl-4-hydroxybenzoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>4-hydroxybenzoate + all-trans-octaprenyl diphosphate = 4-hydroxy-3-all-trans-octaprenylbenzoate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27782"/>
            <dbReference type="ChEBI" id="CHEBI:1617"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57711"/>
            <dbReference type="EC" id="2.5.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="8"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304693.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0581"/>
    <dbReference type="EnsemblBacteria" id="AAZ68615">
        <property type="protein sequence ID" value="AAZ68615"/>
        <property type="gene ID" value="Ecaj_0581"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0581"/>
    <dbReference type="eggNOG" id="COG0382">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034879_1_1_5"/>
    <dbReference type="OMA" id="LVMFSHT"/>
    <dbReference type="OrthoDB" id="1472516at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002083">
        <property type="term" value="F:4-hydroxybenzoate decaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047293">
        <property type="term" value="F:4-hydroxybenzoate nonaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008412">
        <property type="term" value="F:4-hydroxybenzoate octaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01635">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006370">
        <property type="entry name" value="HB_polyprenyltransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PTHR11048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01474">
        <property type="entry name" value="ubiA_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="292" mass="32861" checksum="26E20FF786695562" modified="2005-09-27" version="1">MLCKLKYIVSNFGEYRLLLRLHSVEIIFLAMFPALASIALVSHSVLRACGYMILCVIGAFIMRPAGCIINDIFDRKIDSKVKRTRNRPLANGNLSVVQALKVLGVLLACACLLLACTNMYTVKLSIISMILIVLYPLSKRFFTWPQLLLGIVFNSGVLLGCTMTIGHLTLSAVLLYIGCVFWTIGYDTVYAAQDKEYDIELGLQSTAIKFGNDIRLWIGRLYIITITMWISAGIISMLHPIFYIAILIIGAIFYYQYKKSDFDNPEKCMYMFKVNVYVGLILFLGIVLGRVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRP7</accession>
    <name>FABH_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-oxoacyl-[acyl-carrier-protein] synthase 3</fullName>
            <ecNumber evidence="1">2.3.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">3-oxoacyl-[acyl-carrier-protein] synthase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Beta-ketoacyl-ACP synthase III</fullName>
            <shortName evidence="1">KAS III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fabH</name>
        <name type="ordered locus">Ecaj_0574</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H(+) + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
            <dbReference type="EC" id="2.3.1.180"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. FabH family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.180" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304686.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRP7"/>
    <dbReference type="SMR" id="Q3YRP7"/>
    <dbReference type="STRING" id="269484.Ecaj_0574"/>
    <dbReference type="EnsemblBacteria" id="AAZ68608">
        <property type="protein sequence ID" value="AAZ68608"/>
        <property type="gene ID" value="Ecaj_0574"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0574"/>
    <dbReference type="eggNOG" id="COG0332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_3_1_5"/>
    <dbReference type="OMA" id="WGSEGDK"/>
    <dbReference type="OrthoDB" id="872193at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033818">
        <property type="term" value="F:beta-ketoacyl-acyl-carrier-protein synthase III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01815">
        <property type="entry name" value="FabH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013751">
        <property type="entry name" value="ACP_syn_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004655">
        <property type="entry name" value="FabH_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08545">
        <property type="entry name" value="ACP_syn_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00747">
        <property type="entry name" value="fabH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword id="KW-0276">Fatty acid metabolism</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000056357" description="3-oxoacyl-[acyl-carrier-protein] synthase 3">
        <location>
            <begin position="1"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="ACP-binding" evidence="1">
        <location>
            <begin position="247"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01815"/>
        </source>
    </evidence>
    <sequence length="319" mass="34503" checksum="02AD9694D9FFF08F" modified="2005-09-27" version="1">MKRSLILGIGSYLPKKVVTNDELTLTIETSDEWIVKRTGIKQRHIAEDNEMTSDMATSAAKLALDNAGIDKNEIDLIIVATTTPDRTFPSCATIVQSKLGCKNAFAFDIQAVCSGFVYALSIADNFIKSGQVRTVLVIGAEIMSRILDWQDRSTCVLFGDGAGAIVLSSSTEDDSGIISTNLHSDGTFHDLLYTSGGTAYNGVAGTICMNGTVVFEHAIEKLSASILEILSQNDLEICDIDWFVLHQANIRIIELVARRLKIPYEKMIVSIDQHANTSAASIPLALYYARSSGKLKKHDVAVLAAIGGGLTWGTCLVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRR7</accession>
    <name>Q3YRR7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Polyketide_cyc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0553</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribosome association toxin RatA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304666.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0553"/>
    <dbReference type="EnsemblBacteria" id="AAZ68588">
        <property type="protein sequence ID" value="AAZ68588"/>
        <property type="gene ID" value="Ecaj_0553"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0553"/>
    <dbReference type="eggNOG" id="COG2867">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079653_3_1_5"/>
    <dbReference type="OMA" id="YIDGPFK"/>
    <dbReference type="OrthoDB" id="1691158at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07813">
        <property type="entry name" value="COQ10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="PTHR12901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polyketide_cyc" evidence="2">
        <location>
            <begin position="15"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="154" mass="17942" checksum="DA407EF1A6C71162" modified="2005-09-27" version="1">MSNNEYNLNDDEIINFSAIDLFNIVLDVEKYPDFLPWCKAVYVRTRKENIMIADLLASFKGLSGKYTSHIVFKEPTLNEEGWIKVEAIEGLFKFLHNQWTFIPIDGNRTLVKFYISCAFKVPMLQSAFNLVCDHAYKKIITAFRNRANNLLLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRS6</accession>
    <name>Q3YRS6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Primosomal protein N'</fullName>
            <ecNumber evidence="9">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-dependent helicase PriA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">priA</name>
        <name evidence="12" type="ordered locus">Ecaj_0544</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the primosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the helicase family. PriA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304657.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0544"/>
    <dbReference type="EnsemblBacteria" id="AAZ68579">
        <property type="protein sequence ID" value="AAZ68579"/>
        <property type="gene ID" value="Ecaj_0544"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0544"/>
    <dbReference type="eggNOG" id="COG1198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013353_4_0_5"/>
    <dbReference type="OMA" id="RCHYCGY"/>
    <dbReference type="OrthoDB" id="1132322at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00983">
        <property type="entry name" value="PriA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005259">
        <property type="entry name" value="PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041222">
        <property type="entry name" value="PriA_3primeBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042115">
        <property type="entry name" value="PriA_3primeBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041236">
        <property type="entry name" value="PriA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040498">
        <property type="entry name" value="PriA_CRR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17764">
        <property type="entry name" value="PriA_3primeBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18074">
        <property type="entry name" value="PriA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18319">
        <property type="entry name" value="PriA_CRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00595">
        <property type="entry name" value="priA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="142"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="406"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="371"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="398"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="658" mass="74565" checksum="016D2096B878018C" modified="2005-09-27" version="1">MIAEVLLPVPINKTFYYSIPENESLFIGEYVLVPFGVRTLVGIVLKLSNSKPLDCGIEKLQLKVIISKSSLPRINPTLLNFIKWVSEYNVVPAGLVLKMVFSNVINAKSFSSLKFSVKKNDKYNDVAISLSDEQRFAYDDIIDKVSGYSVIVLDGETGSGKTEVYCEVIRELIKRDETAQALILLPEIVLTLQLIKRIKRYFDSYYPVEWHSNLTLKNRREYWLSVAYGQSLIVIGARSALFLPYNNLKVIIVDEEHDSSFKQESGVLYNARDMSIVLAKNLNIPIILSSATPSLETINNVLKSQYYHVKLTKRFGNAKLPSVKIVDLCKSKMVNTWLSYELYNNILKVLERQDQVMLFLNSRGYAKLRLCRACGFKMSCKNCSTWLIEHKKKNVLLCHYCGYSCAMQNQCSNCLCESSLISYGVGVERVAEEIVALIPDAKIAVISSDVSSKDIGIMVDLIMRNEVNVIIGTQVIAKGHNFPKLTFVGVVDADLGLNNSDLRATERTYQLLHQVSGRSGRFVDNGEVMLQTYDVNSALIKSLLSYNRELFYNLELESRSLTKMPPYTRLIGIIVSGEHEPDVVNVSKRIVNTLPHTLTVLGPVPAPISFLNRRYRYRILIKVQNNVMIQKLLAKYKEYYSKVNKIKITIDVDPVNFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRT9</accession>
    <name>Q3YRT9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TrbL/VirB6 plasmid conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0530</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TrbL/VirB6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304644.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0530"/>
    <dbReference type="EnsemblBacteria" id="AAZ68566">
        <property type="protein sequence ID" value="AAZ68566"/>
        <property type="gene ID" value="Ecaj_0530"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0530"/>
    <dbReference type="eggNOG" id="COG3704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5644">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004452_0_0_5"/>
    <dbReference type="OMA" id="EEPYNND"/>
    <dbReference type="OrthoDB" id="290497at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030255">
        <property type="term" value="P:protein secretion by the type IV secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007688">
        <property type="entry name" value="Conjugal_tfr_TrbL/VirB6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04610">
        <property type="entry name" value="TrbL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="649"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="677"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="743"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="779"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="819"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="908"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="978"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1419"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1023"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1066"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1131"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1292"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1342"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1421"/>
            <end position="1444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1444" mass="157469" checksum="C1AC66E267CF0D63" modified="2005-09-27" version="1">MKKLIILLLFFITGCGIGCIEGSKGVSGTSIAIEVKPVVEKVSDVLQPDWIDTGLTVNESQPPVKLTILGAINMCPKDSINPKDIIVPAVACAHGTYDAKDESIYKDMKSTCSSYGEVATTTLPIETFYLGKGDTFKVSLIPRKMTVTDCNADKLKELGIIYYSDNEIFEDSGCNTPIKAEDICRTGIAKFYVKIEDKCSSFDGSISSIPRLQGTEVSALVDNTDIPHGNKVFYSEDVKKQWIKTPFIYDAKVIKGKNIDTEALKKHCEVIKKLSTTGIINDSANSTISQLEFYKNQKKLKNSKDSKQKDNKQSEPDVLKDKKEDDIQEIIDIINNFTTYDINCACGTICNSKAALKGIATVSRHDESGNCIINNGDSFNKNTKLEDITEEELEKMANNASDEVIQGLTAVFITKSGDKNNVKYHCMPSKNESNACYANIFSDNVGSTSLKSGFNYKYEANDPAKLGFAIIGQQQHYNSYYGGYNIQVERICNFMYGKKLYMYIGSKAPTIFPGEKDTIELFVPEKDDAESGTGMYTINADGTEKKSGKVYLGIDTRGYEDQFDYSSLPSLESENKYTVNFFIKKWNPNFSKVFVMIRDSLLGILYGLPKNSNASSISQAVEIVRTSKSKGAVQSIYTNQTGAGSLWRALQALCTLYIIFTVLGYVLGVVKCTKYDLGVRIAKIAIIVGLISQGSWEFFSDHFFSIFVQGVSDLIAAFNGELDGDNSFKFLDTTIGVLLAGETWIRLATLILTGPIGWIVFITVIWSFFIFFVSVIEAVIAYLFTVVAIAFLATLAPIFITFIFFQLTKTLFDSWIKMLVNFSLQPIILFAGLAFLNQVMLTVLHAVTGFTVCNQCFLGFDLPIGVTEKGAPPDICIVSVLLPIGYSTELSMNDSIREGYANDNEGSFLGLPFGITAVLMLLLISNAMKGFKGMSETMAHSISGSVAGLSASIHAATQSLASMVGLDQETQSIIKDALRNRRSTGKSDVNIVGRTDTNPESKGEIVGKDGDDNGPLPSDPRSHSGDVHGDGDVSDKEGANAGPKSESGNNVLDGGKGDVSANSGRVDGHLASSGNDVLDTTPSNYDGAQDASNSGNVDNSTVEKYDSNDDTISVSSEGSYQHKEEEPYNNDSDTSSVSSEGSYQPKEEEPYNNDSDTSNVSSEDSYQPKEEELYNNDSDTSSVSSEDSYQPKEEEPYNNDSDTSSVSSEDSYQPKEEEPYNNDSDTSSVSSEDSYQPKEEEPYNNDSDTSSVSSEDSYQPKEEEPYNNDGDTSSVSSEDSYQPKEEEPYNNDSDTSSVSSDNGDTNSSSGDSSVEDLHSTSTDSEPQSPAVSDVAETPDVGDASDSSLNNATNNNENTGENNNSDIQAEEGKASVVVDPLSGDDNDGKRGKQDINFLEGPQKRRLYMDEHSSVAQKIAGHESAIEVEKESHERSIVKKRGKKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRU1</accession>
    <name>Q3YRU1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304642.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0528"/>
    <dbReference type="EnsemblBacteria" id="AAZ68564">
        <property type="protein sequence ID" value="AAZ68564"/>
        <property type="gene ID" value="Ecaj_0528"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0528"/>
    <dbReference type="eggNOG" id="COG5448">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114534_0_0_5"/>
    <dbReference type="OMA" id="EQRNINW"/>
    <dbReference type="OrthoDB" id="2005986at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011740">
        <property type="entry name" value="DUF2460"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09343">
        <property type="entry name" value="DUF2460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02217">
        <property type="entry name" value="chp_TIGR02217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="199" mass="23311" checksum="ADA8BF08BF3FAE9B" modified="2005-09-27" version="1">MTFHEIRFPEDISYGSTGGPEFSTSIVQTNNGSEYRKVNLSYSRNKYNIMYNVKSEDQLLKLINFFYVHKGKAIGFRFKDWSDYKAKKEHIGIGNDKQKKFQIIKTYSIDQHSYIRKITKPVINTVKIYYDDIEKVDGFSVNFSNGQIEFNTPPHNGVTIYADYEFDVPVRFDSDYLPYSIDNYNEHNCNNIALIEIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRU5</accession>
    <name>Q3YRU5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 9">GTPase Der</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GTP-binding protein EngA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">der</name>
        <name evidence="11" type="ordered locus">Ecaj_0524</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">GTPase that plays an essential role in the late steps of ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304638.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0524"/>
    <dbReference type="EnsemblBacteria" id="AAZ68560">
        <property type="protein sequence ID" value="AAZ68560"/>
        <property type="gene ID" value="Ecaj_0524"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0524"/>
    <dbReference type="eggNOG" id="COG1160">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016077_6_2_5"/>
    <dbReference type="OMA" id="KFRFLEY"/>
    <dbReference type="OrthoDB" id="263682at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00195">
        <property type="entry name" value="GTPase_Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031166">
        <property type="entry name" value="G_ENGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016484">
        <property type="entry name" value="GTP-bd_EngA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032859">
        <property type="entry name" value="KH_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14714">
        <property type="entry name" value="KH_dom-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006485">
        <property type="entry name" value="GTP-binding_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03594">
        <property type="entry name" value="GTPase_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51712">
        <property type="entry name" value="G_ENGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="EngA-type G" evidence="10">
        <location>
            <begin position="174"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="55"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="117"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="180"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="227"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="292"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="439" mass="49623" checksum="60EC9D7A11800684" modified="2005-09-27" version="1">MLKIAIVGLPNVGKSTIFNRLTNQKSAIVSNTPNLTRDRREGNADLCGLKFKVIDTGGVDNTIKLSALVLGQVKLAIEGCDIVFFVVDAKVEQDDKNLEFAKYLRKSTQKPVILVANKCESQKKCYTVDYLGHFDFIGPVYISAEHNLGLIDLYEVLLPFVGEHDLDILKSDNIRLSIVGRPNAGKSTFVNSLLGESRMIVSPEAGTTRDSIDIEYQYKDQMFTLIDTAGMRKKAKVIEEIEVSSVHKTIGSINRSDIVVLVIDSVYGIEQQDLYIADLAIQNGKALIVALNKWDMIARKDRSELLKDICNYNKLNFEVPIVEISALKNINCNKVIDKSIGLYKYLKMRISTPMLNKWLKLAVDYHKPPLFHGKVVKLKYITQVRVMPPAFIIVVNNLNAIDLTYQQYLMRSLRKHFSINEIPIKLNLKKNKNPYDNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRV6</accession>
    <name>Q3YRV6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304627.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0513"/>
    <dbReference type="EnsemblBacteria" id="AAZ68549">
        <property type="protein sequence ID" value="AAZ68549"/>
        <property type="gene ID" value="Ecaj_0513"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0513"/>
    <dbReference type="HOGENOM" id="CLU_958882_0_0_5"/>
    <dbReference type="OMA" id="RETELHF"/>
    <dbReference type="OrthoDB" id="1353262at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="290" mass="33928" checksum="B18A70E31228FAD6" modified="2005-09-27" version="1">MPSAKKLIARAIGLVIGSLLLIFSFNILMRYFSKKVNSIFLGICLLLSIGITVFFKIYGIDKTSSTFINLKIYGMLVSSALIMFCIANLILKKSSECSVKLSKQNKQIYYLNINLDNLKIELNIIKREIEWYFTHLCNDDKIYINIEKEHLVSTNKKYYDLNSLCKVVKLITFIVALLIFLYQFFYSCINQKFRSVELNMYYALLSLFFCIFMVDKLPDYYYEYVKTNADEDNKNIIDSLNNELRIKTKLRDSLLKEVSAYNNTIDDESLIGESDYQLNRQKILKILNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRV9</accession>
    <name>Q3YRV9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cytochrome c1</fullName>
            <ecNumber evidence="10">1.10.2.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0510</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.10.2.2" evidence="10"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304624.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0510"/>
    <dbReference type="EnsemblBacteria" id="AAZ68546">
        <property type="protein sequence ID" value="AAZ68546"/>
        <property type="gene ID" value="Ecaj_0510"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0510"/>
    <dbReference type="eggNOG" id="COG2857">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040334_1_2_5"/>
    <dbReference type="OMA" id="VWVKKFK"/>
    <dbReference type="OrthoDB" id="1426747at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002326">
        <property type="entry name" value="Cyt_c1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266">
        <property type="entry name" value="PTHR10266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02167">
        <property type="entry name" value="Cytochrom_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00603">
        <property type="entry name" value="CYTOCHROMEC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230976" evidence="8">
        <location>
            <begin position="22"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="9">
        <location>
            <begin position="46"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="251" mass="28377" checksum="D929C7CFEF5B5EE1" modified="2005-09-27" version="1">MLIRYLLIVVCFICISSICVADEFKHTSPKKVNWKFDGILGSFDRQSIQRGYQVYREVCSSCHSMRRIAFRNLRDVGFSEDEVKAIAASYQVLDGPNDNGEMFERPGIQSDYFVSPFPNKEAAKAANKGAFPPDLSLMIKARHNGANYVYSLLTGYESSEPDEGGLYTNPYFSTGKILMAPPLSDNLVQYTDGTDSKIENMAYDLVNFLQWAAEPEMEVRKKLGIKVISFLLILTIFVILVNKGLWKSLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRW0</accession>
    <name>Q3YRW0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304623.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68545">
        <property type="protein sequence ID" value="AAZ68545"/>
        <property type="gene ID" value="Ecaj_0509"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0509"/>
    <dbReference type="eggNOG" id="COG3064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026149_0_0_5"/>
    <dbReference type="OrthoDB" id="373711at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230978" evidence="1">
        <location>
            <begin position="24"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="538" mass="60958" checksum="7B3A83DEF2D1D9CA" modified="2005-09-27" version="1">MKNPLIISALASLLMMCSNSAFSDEMLNDMSAVDKNCGSEIDIANCKKFIGNVKKCVLLSEMNSAIKKSSGLKFSGNVVSYGWYSSDSSKSTKKFLNHTRLFDVDTGGNIEKIGTKHDAILSLRVESNPDKHGIVYGIHSQVNIPNVAGRSFNRHAAFNRGSKVFAKTPYGDFSAGYQEGMESVIKLNAFDIAAGDNYNIWAKHLRGIVHEKKESPSYLLYNFNFNPGLYSESVFRNDDNLVFDKSNHNASFGIITKDFVNNLPFRLSYQSQNFMGLRFGISYSPAGYNQQLFEFPVIFGRYLVSDVKKKEDGKLDVTKDYTLVYGVNRRSDTSNVGIVGPVYKNIISGGVSYAYNVKNFKFNASIIGEYGNEYIDNDTRLSYAMYYVHQQLRGISIGFNFSYDNLRLAGAYGNLNRSGFLNKICYSHYTTQNTCYDKWQFSKNTNYYWNIGIAYQREPLNFSIIYFESSRLGNKLKDVGVGLQYNILKYNSFKNSLFINYNYYIFDQMNYASNSVYYRPTDLSGKGYVLLAGIKSEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRW2</accession>
    <name>Q3YRW2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304621.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68543">
        <property type="protein sequence ID" value="AAZ68543"/>
        <property type="gene ID" value="Ecaj_0507"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0507"/>
    <dbReference type="eggNOG" id="COG3064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026149_0_0_5"/>
    <dbReference type="OrthoDB" id="373711at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004231013" evidence="1">
        <location>
            <begin position="24"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="490" mass="54661" checksum="3A8411374EE1DC28" modified="2005-09-27" version="1">MKKFSFATAFVSFLLLYNVDAFSVDDMDQDHKTQKKLVDVVKNSEHIKQVSLYPSMKGYHKIDVGGKILSYAWFTNHSKKIQDHGVKLDGILNIKSVNNNADLGIYYGGNFQVAIPAVKNKNFIPSMKAYNRGAQLFVESSYGNLSFGYQEGVESIMKIDASSIGAGDNSVAWLEYTDLSSINSIKYHVFPGLYSESVFNRSNSDIISQDKDFINNLPFRVSYQSPSFMGVRFGVSYAPTGYDTSLFENVSSHTIKKFKFPPLLTEEMVSELKDPNTLNGALEKLSSPSIQDVEVESIVPAKIDFVGAHYENIVSAGLSYSNSFNDIDFSASIVGEYGSNSADKLKKLYNTYSSAEELAAFAVGTSITYRNAVFAASYGYLGRSGYISAIYPDTVTFEPASKSDYTYYWNIGAKYMYNKASVSTSYFRSNKVGTQFYDFSLGVDYNLSVNSNCKGQYKIFGNYHYFNVDNKSSKIKHDGNVVLLGVKYEF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRW8</accession>
    <name>RLME_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName>Ribosomal RNA large subunit methyltransferase E</fullName>
            <ecNumber>2.1.1.166</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>23S rRNA Um2552 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>rRNA (uridine-2'-O-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rlmE</name>
        <name type="synonym">ftsJ</name>
        <name type="synonym">rrmJ</name>
        <name type="ordered locus">Ecaj_0501</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = 2'-O-methyluridine(2552) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42720"/>
            <dbReference type="Rhea" id="RHEA-COMP:10202"/>
            <dbReference type="Rhea" id="RHEA-COMP:10203"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.166"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.166"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRW8"/>
    <dbReference type="SMR" id="Q3YRW8"/>
    <dbReference type="STRING" id="269484.Ecaj_0501"/>
    <dbReference type="EnsemblBacteria" id="AAZ68537">
        <property type="protein sequence ID" value="AAZ68537"/>
        <property type="gene ID" value="Ecaj_0501"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0501"/>
    <dbReference type="eggNOG" id="COG0293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_4_4_5"/>
    <dbReference type="OMA" id="FRSTEYH"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155493" description="Ribosomal RNA large subunit methyltransferase E">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="171" mass="19554" checksum="0E612275C3E07A66" modified="2005-09-27" version="1">MDNKFSIFKKGQYVLDLGSFPGGWSQFAAQKVSHGNNNLVFSVDIQNMDAIPNVIFIKCDISNEIEILNDKFHNKKFDVILSDMAPKACGNKQVDHANIINLCEMSLDIVIKFARENGTFITKILQGEYEKEFYQSMKSNFKLVKYFKPKASRKDSSEIYLVGLGFKKDFQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-09" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRY0</accession>
    <name>RL21_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplU</name>
        <name type="ordered locus">Ecaj_0488</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds to 23S rRNA in the presence of protein L20.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L20.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304603.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRY0"/>
    <dbReference type="SMR" id="Q3YRY0"/>
    <dbReference type="STRING" id="269484.Ecaj_0488"/>
    <dbReference type="EnsemblBacteria" id="AAZ68525">
        <property type="protein sequence ID" value="AAZ68525"/>
        <property type="gene ID" value="Ecaj_0488"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0488"/>
    <dbReference type="eggNOG" id="COG0261">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061463_3_2_5"/>
    <dbReference type="OMA" id="HRQEITR"/>
    <dbReference type="OrthoDB" id="1949059at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01363">
        <property type="entry name" value="Ribosomal_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="L21-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="L21p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001787">
        <property type="entry name" value="Ribosomal_L21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018258">
        <property type="entry name" value="Ribosomal_L21_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="PTHR21349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="SSF141091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00061">
        <property type="entry name" value="L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01169">
        <property type="entry name" value="RIBOSOMAL_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000270662" description="50S ribosomal protein L21">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01363"/>
        </source>
    </evidence>
    <sequence length="102" mass="11786" checksum="3A3514C43A4025DC" modified="2005-09-27" version="1">MFAVVETGGKQYKVKEQDVIRIEKLNASVGEEVTLSNVIALTDVNNNIVFTQSATVTASVLEQCRNDKIIIFKKKRRKNYRRKNGHRQYMTVLRVIKINNME</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRZ8</accession>
    <name>Q3YRZ8_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec-independent protein translocase protein TatC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tatC</name>
        <name evidence="6" type="ordered locus">Ecaj_0470</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. Together with TatB, TatC is part of a receptor directly interacting with Tat signal peptides.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The Tat system comprises two distinct complexes: a TatABC complex, containing multiple copies of TatA, TatB and TatC subunits, and a separate TatA complex, containing only TatA subunits. Substrates initially bind to the TatABC complex, which probably triggers association of the separate TatA complex to form the active translocon.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TatC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304585.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0470"/>
    <dbReference type="EnsemblBacteria" id="AAZ68507">
        <property type="protein sequence ID" value="AAZ68507"/>
        <property type="gene ID" value="Ecaj_0470"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0470"/>
    <dbReference type="eggNOG" id="COG0805">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031942_1_0_5"/>
    <dbReference type="OMA" id="EMSFLDH"/>
    <dbReference type="OrthoDB" id="1848013at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019820">
        <property type="entry name" value="Sec-indep_translocase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="PTHR30371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00945">
        <property type="entry name" value="tatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01218">
        <property type="entry name" value="TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="250" mass="28854" checksum="A632BE41A10E1535" modified="2005-09-27" version="1">MKSTTGEMPFFKHFEELRYRLFFCFAFFCIMFGVCYFFSERIYNFLLIPLIDLEGANSEFSLIYTDLTEAFFVYLKVATMAALLCSFPVFAWQFYMFLAPGLYKKEKLVLLPYLISTPVLFVIGAAIVYYYIFPLAWRFFIAFENRDASMGVPIEFMPSVSEYLELVLQLMFAFGVAFQIPIFLTLMSRIGIVSAKSLAKKRRVAIVVIFILAAILTPPDVLSQIGLAIPMLFLYEASILACKYIERHDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-06-16" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS11</accession>
    <name>MIAA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dimethylallyl diphosphate:tRNA dimethylallyltransferase</fullName>
            <shortName evidence="1">DMAPP:tRNA dimethylallyltransferase</shortName>
            <shortName evidence="1">DMATase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl-diphosphate:tRNA isopentenyltransferase</fullName>
            <shortName evidence="1">IPP transferase</shortName>
            <shortName evidence="1">IPPT</shortName>
            <shortName evidence="1">IPTase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">miaA</name>
        <name type="ordered locus">Ecaj_0457</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = diphosphate + N(6)-dimethylallyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304572.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS11"/>
    <dbReference type="SMR" id="Q3YS11"/>
    <dbReference type="STRING" id="269484.Ecaj_0457"/>
    <dbReference type="EnsemblBacteria" id="AAZ68494">
        <property type="protein sequence ID" value="AAZ68494"/>
        <property type="gene ID" value="Ecaj_0457"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0457"/>
    <dbReference type="eggNOG" id="COG0324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032616_0_1_5"/>
    <dbReference type="OMA" id="YAKRQYT"/>
    <dbReference type="OrthoDB" id="1069591at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="PTHR11088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000377151" description="tRNA dimethylallyltransferase">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00185"/>
        </source>
    </evidence>
    <sequence length="300" mass="34563" checksum="F357107A837DEB3C" modified="2005-09-27" version="1">MNNVLIITGPTASGKSKISVKIAQDNNGIIVNCDSKQIYKEIPIITDQPKLNDYSVKHRLYGYISVTQQYSVGLWIEDLKKEILSATAEKKLPIITGGSGMYINSIIYGLSQIPKIEDDVRYKTKKLFEDLGNKEFYALLINKDPLAKCLHQNNSHRLLRAYEVIEQTGISIFSWKKNTIRQPILNNFKLCVLLPPRDQVYKTINERFINMINTDVIEEIENLLSLNVPKHFPAMKAHGVPELISYLKNKISIDEAIETAQKNTRHYAKRQYTWFKNQFPNASFYDSKCQLLESIKNYQN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS14</accession>
    <name>SYS_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA synthetase</fullName>
            <shortName evidence="1">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">serS</name>
        <name type="ordered locus">Ecaj_0454</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304569.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS14"/>
    <dbReference type="SMR" id="Q3YS14"/>
    <dbReference type="STRING" id="269484.Ecaj_0454"/>
    <dbReference type="EnsemblBacteria" id="AAZ68491">
        <property type="protein sequence ID" value="AAZ68491"/>
        <property type="gene ID" value="Ecaj_0454"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0454"/>
    <dbReference type="eggNOG" id="COG0172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_1_1_5"/>
    <dbReference type="OMA" id="SPCFRRE"/>
    <dbReference type="OrthoDB" id="353391at2"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43697">
        <property type="entry name" value="PTHR43697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000019676" description="Serine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="228"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="259"/>
            <end position="261"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="346"/>
            <end position="349"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <sequence length="423" mass="48480" checksum="4805DB9E312C6304" modified="2005-09-27" version="1">MHDVDFIKRNPELFDYAMQNRNYEKVAQKIIELDIKKKQLLTQLYSLQKERNQITREIEKLKKDGVKCDTQIESSKNITEKINDINNMIKEDSQLIDLLNVLPNVPDDIVPVGKDESSNVEIRKHGYKRNFDFQVKTHYELGEKLNLMDFKQAAKLSGSRFVILKNQLAQLDRALANFMLDIHTKEFGYSEISHPILVHESAMYGVGQLPKFADDSFKTTENFRLVPTSEVALTNLVSGMNINSCDLPIRLTACSPCFRLEAGSAGKDTRGMMRQHQFNKVELVSIVTEEQSASELERMVQVAEEVLKRLELPYRVMMLCTGDMGFSASITYDIEVWVPSQNRYREISSCSNCKDFQARRMNTKYTTITNNVKVSKFAHTLNGSALAIGRTIIAILENYQNMDGSITIPLALRKYMNDQEFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS19</accession>
    <name>Q3YS19_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Coproporphyrinogen oxidase</fullName>
            <ecNumber evidence="6">1.3.3.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0446</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.3" evidence="6"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304564.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0446"/>
    <dbReference type="EnsemblBacteria" id="AAZ68486">
        <property type="protein sequence ID" value="AAZ68486"/>
        <property type="gene ID" value="Ecaj_0446"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0446"/>
    <dbReference type="eggNOG" id="COG0408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026169_0_1_5"/>
    <dbReference type="OMA" id="HDKGTLF"/>
    <dbReference type="OrthoDB" id="1086432at2"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001260">
        <property type="entry name" value="Coprogen_oxidase_aer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036406">
        <property type="entry name" value="Coprogen_oxidase_aer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018375">
        <property type="entry name" value="Coprogen_oxidase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755">
        <property type="entry name" value="PTHR10755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01218">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000166">
        <property type="entry name" value="Coproporphyri_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00073">
        <property type="entry name" value="COPRGNOXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102886">
        <property type="entry name" value="SSF102886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01021">
        <property type="entry name" value="COPROGEN_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="282" mass="32816" checksum="2865AA59EB4A17C7" modified="2005-09-27" version="1">MEEKQKRAVNWFTTLQNQLLSKFISIEKEFSSFSPQVDNKNWERPGGGGGHSIIIYGKVFEKAGINVSEVYGDITKTHELSNVLNNSEIFNSSSEKKFWASGISVVAHMCSPFVPAIHMNTRFICISDKSWFGGGVDLTPTYKNTQDQELFHDSLRKICDQHNKEYYSKFKQQCDEYFFLHHRQEPRGIGGIFYDNINSGNWEDDFQYTQAVGKFLLDIYPIIIRRNLNKKWTVEEREYQLIKRGRYVEFNLIYDRGTKFGLLTNGNPDAIMMSMPPVVKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS20</accession>
    <name>Q3YS20_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Porin_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0445"/>
    <dbReference type="EnsemblBacteria" id="AAZ68485">
        <property type="protein sequence ID" value="AAZ68485"/>
        <property type="gene ID" value="Ecaj_0445"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0445"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_723069_0_0_5"/>
    <dbReference type="OMA" id="SGYIKQD"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004231019" description="Porin_4 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="2">
        <location>
            <begin position="21"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="382" mass="42125" checksum="F9DFBB4CDAF3D953" modified="2005-09-27" version="1">MCNKILYMSAISCFALLMNQAMADNLSVNGSLKLQYGINSKDFSRMSISANPNLTYSYDINDYVTIGSHVKLGTNLITNGANDATGSLNKNILDINSSTSSAYIFLKSPYGGNIQIGLTDPVAQSMKTSSIGSNGNFSQYISLKNDSRFPYGYNLDDTILVEPSIYSAYYGFSVPVISYYSPKIKGLEIGCSYIFKDKNGDVGYRNIFDIGVKYENNLFSDINYSISGTIEHAVKALSSDNVKFNDLLAYSTGISVKYKNFTFAGSYGNLGKSGKEEITISNTSGSNDTKKFKVISSHFYDLGIKFETNIFNVSANYFYSSKKNSIDNKEDNHSLSVYTLGLEKVLDDKLSFYADIIYFTVDRPTLSNNSGYLTLIGAKLSF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS31</accession>
    <name>SYE1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate--tRNA ligase 1</fullName>
            <ecNumber evidence="1">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-tRNA synthetase 1</fullName>
            <shortName evidence="1">GluRS 1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gltX1</name>
        <name type="ordered locus">Ecaj_0432</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304552.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS31"/>
    <dbReference type="SMR" id="Q3YS31"/>
    <dbReference type="STRING" id="269484.Ecaj_0432"/>
    <dbReference type="EnsemblBacteria" id="AAZ68474">
        <property type="protein sequence ID" value="AAZ68474"/>
        <property type="gene ID" value="Ecaj_0432"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0432"/>
    <dbReference type="eggNOG" id="COG0008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_6_0_5"/>
    <dbReference type="OMA" id="WDEGPFF"/>
    <dbReference type="OrthoDB" id="1409413at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000237360" description="Glutamate--tRNA ligase 1">
        <location>
            <begin position="1"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="1">
        <location>
            <begin position="238"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00022"/>
        </source>
    </evidence>
    <sequence length="469" mass="53980" checksum="F265F659A7DB3D4A" modified="2005-09-27" version="1">MSHDVITRFAPSPTGHLHLGGARTALFNWLYAKHNNGKFLLRIEDTDKKRSSQELIDSIINAMSWLKIHYDGKAVLQSENISRHVEIANQLMLNNKAYYCYCSEEEINKEKEESAKKGLYYKHNCIWKNKSAPNSNITRVVRLKSPTDGVTSFDDEVYGNITVNNSQLDDMILLRSDNTPTYLLSVVVDDHDMNISHIIRGTDHLTNTARQLLIYNALEWDPPKFAHIPLIHDENGNKLSKRHQAIGIHEYKNSGILPEAIFNYLLRMGWSHENDEVITIDQAIRWFSIEGIGQSPSRLDSKKLEFLNNHYINITDNETILNMIIPIIEEKTGHIITDVKKGYLLKGLSELKKRTKNLVNLAKESLFYVEDIPISIDQESITVIKDYKHIFSILYDNLSKISEKDWNHNILMSIIKDISCNLEVKISIVYHCLRASITGRMNAPSIIEIMINLQHKECLQRIKYALDVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS50</accession>
    <name>Q3YS50_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase_S15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304533.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0412"/>
    <dbReference type="ESTHER" id="ehrcj-q3ys50">
        <property type="family name" value="Atu1826-like"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68455">
        <property type="protein sequence ID" value="AAZ68455"/>
        <property type="gene ID" value="Ecaj_0412"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0412"/>
    <dbReference type="eggNOG" id="COG2945">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086287_0_0_5"/>
    <dbReference type="OMA" id="MDWARPQ"/>
    <dbReference type="OrthoDB" id="1090356at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000383">
        <property type="entry name" value="Xaa-Pro-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02129">
        <property type="entry name" value="Peptidase_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S15" evidence="1">
        <location>
            <begin position="41"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="241" mass="26955" checksum="9B564C00E2EE5D67" modified="2005-09-27" version="1">MREIFFNGAVGKIEGKYHHNKTLGAPLALIFHPHPQYGGNMDNKIVYNLYNIFANNGFSVLRINFRGVGKSSGNFEKGIGELSDGAAAADWLQNNNVASAPFWVAGFSFGAWVAMQLMMRRPEVEGFIAVSPPANKYDFSFLSPCPVPGLIIQGDQDSISDEAAVSQLAARLSNSIKSEYMQYYVIEKADHFFRDHLDKFNEIVDNYIKFRLSESTGSNKKGVVHKKVKQRRIIMHDDPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSA0</accession>
    <name>Q3YSA0_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Signal peptidase I</fullName>
            <ecNumber evidence="3 7">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ecaj_0362</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase S26 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="3 7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304483.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0362"/>
    <dbReference type="MEROPS" id="S26.001"/>
    <dbReference type="EnsemblBacteria" id="AAZ68405">
        <property type="protein sequence ID" value="AAZ68405"/>
        <property type="gene ID" value="Ecaj_0362"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0362"/>
    <dbReference type="eggNOG" id="COG0681">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028723_1_2_5"/>
    <dbReference type="OMA" id="ACDAYIK"/>
    <dbReference type="OrthoDB" id="1741894at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019757">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Lys-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019756">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="PTHR43390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00501">
        <property type="entry name" value="SPASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00760">
        <property type="entry name" value="SPASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S26" evidence="9">
        <location>
            <begin position="8"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="236" mass="26858" checksum="E01CB9CF325149BD" modified="2005-09-27" version="1">MKKRGSFIKFVLTLSCALTVALLLRTFLFEPFHIPSGSMKSTLLVGDYVFVSKYSYGYSRYSIPFSLPIITGRIFSKLPKAGDVVVFRPPKQPNLHYIKRVIGVPGDKIQLINGFLYVNGNKMKYEKLGDFIDDDGKVITRYLETLYNGNTHEVLDEVQDSSLDNTPLYKVPEGHIFVLGDNRDNSRDSRFITDVGYIPLENVVGKAHVVALSFKKSEIRFLPFAIRLDRIWHTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSB7</accession>
    <name>Q3YSB7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0345</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304466.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68388">
        <property type="protein sequence ID" value="AAZ68388"/>
        <property type="gene ID" value="Ecaj_0345"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0345"/>
    <dbReference type="HOGENOM" id="CLU_433287_0_0_5"/>
    <dbReference type="OrthoDB" id="2107197at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="631" mass="68618" checksum="7FA955E08F597F23" modified="2005-09-27" version="1">MKISYKSTLSVVGIVAAALSVTLVCIYCSKVIPNNKSNIIPVLFGIVLLSGVAIACLVIICCCKNSLLDVDDERISINSNRNGYVSIDSDNASIGSHDQYLDVNDQIKKDFENVIYKFNYLLSNIERCDVQNVGGSQFRFGNVKTVFFFPVFKNNFDVMLNNLLSKGLLTEHAVNDQRIVNELNPEDYSFVCIAAKLIMNTSLCEVYPLWVRNCIKVLAVLPINDIGMVQRRLTCDKIISLLGQRVYFANSYKDLGEKFAELMNSIKVHGIRTVEDLYKIWDNPKLAQYVDQLSQLRYFRYYNFFGVDCDQAKADLAFQILDSYLINGGMPLEVASKYADAVQKLESAKLAKERTKQDTREMASGIELTTMELNKIKAGKLVAEYAVDHINRTTISWVGSSLNANLVDVQFTKNCTSDLIRRVASTARPFEVSSAMRVGDRTQAINSSYPESSVAAGIPKASASSRMMARSVVGHSSTMYGGSSAQASTSGYTLGVLSGGDQANADTAGPFEFSSAMSVGDRTQAINSSYPESSVATGIPKIDTSGRKQSKPITKASSTTHVDSRTRASSSIASSVTGIPKASAGSRMMSRPVAQTLCTTHVGSRATAGSSSCTASSVATDRPKIDTSGRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSC6</accession>
    <name>TRUB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="1">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="1">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truB</name>
        <name type="ordered locus">Ecaj_0336</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304457.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSC6"/>
    <dbReference type="SMR" id="Q3YSC6"/>
    <dbReference type="STRING" id="269484.Ecaj_0336"/>
    <dbReference type="EnsemblBacteria" id="AAZ68379">
        <property type="protein sequence ID" value="AAZ68379"/>
        <property type="gene ID" value="Ecaj_0336"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0336"/>
    <dbReference type="eggNOG" id="COG0130">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_0_3_5"/>
    <dbReference type="OMA" id="ELQFIRW"/>
    <dbReference type="OrthoDB" id="1166299at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02573">
        <property type="entry name" value="PseudoU_synth_EcTruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="PTHR13767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00431">
        <property type="entry name" value="TruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000229357" description="tRNA pseudouridine synthase B">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01080"/>
        </source>
    </evidence>
    <sequence length="301" mass="33614" checksum="C4BCFFD12A116474" modified="2005-09-27" version="1">MYGWVNLDKPCGMSSALAVNLVKRILNVKKAGHAGTLDPLASGVLPIAIGEATKVMPYAVDVIKSYLFTVQWGEQRTTDDAEGEIVDKSDMIPCVENIKKIIPDFIGLLKQVPPSFSAVHVNGVRAFELARSGQDVNLSSRFVDVLELKLLSFDVENNKADFYLSCRKGVYVRSIARDLGIKLGCLGYVTKLQRVRVGCFRKKNAITLEMLKTLYSTNCKSSYLLPLWYVLQDIKHLNNFFSELEIKKLKNGQNIELNNLYVIRNCDICYVSTGNVPVAICSIVNSVVRPVRIFNVRGLVF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSD5</accession>
    <name>ACPS_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Holo-[acyl-carrier-protein] synthase</fullName>
            <shortName evidence="1">Holo-ACP synthase</shortName>
            <ecNumber evidence="1">2.7.8.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4'-phosphopantetheinyl transferase AcpS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">acpS</name>
        <name type="ordered locus">Ecaj_0326</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>apo-[ACP] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="EC" id="2.7.8.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the P-Pant transferase superfamily. AcpS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304448.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSD5"/>
    <dbReference type="SMR" id="Q3YSD5"/>
    <dbReference type="STRING" id="269484.Ecaj_0326"/>
    <dbReference type="EnsemblBacteria" id="AAZ68370">
        <property type="protein sequence ID" value="AAZ68370"/>
        <property type="gene ID" value="Ecaj_0326"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0326"/>
    <dbReference type="eggNOG" id="COG0736">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089696_1_2_5"/>
    <dbReference type="OMA" id="DERHYAV"/>
    <dbReference type="OrthoDB" id="1893660at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="SSF56214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00516">
        <property type="entry name" value="acpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword id="KW-0276">Fatty acid metabolism</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000228285" description="Holo-[acyl-carrier-protein] synthase">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00101"/>
        </source>
    </evidence>
    <sequence length="126" mass="14366" checksum="52B54AF7E59B5E08" modified="2005-09-27" version="1">MILGVGTDIVYIPRISNLWKKFGIKFLTRVFNQKEIEDSYKYTNFDAQVRHFAKRFAAKEAYVKALGTGFGQSIKMSDIVILNNPYGKPQITVSNSNPIHKIELSISDEKDYAIAFIVIYNANIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSE1</accession>
    <name>Q3YSE1_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="4">O-methyl transferase, family 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304442.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0320"/>
    <dbReference type="EnsemblBacteria" id="AAZ68364">
        <property type="protein sequence ID" value="AAZ68364"/>
        <property type="gene ID" value="Ecaj_0320"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0320"/>
    <dbReference type="eggNOG" id="COG4122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067676_4_0_5"/>
    <dbReference type="OMA" id="SVARRYW"/>
    <dbReference type="OrthoDB" id="1948290at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="219" mass="24745" checksum="1A3D63C4671E80AF" modified="2005-09-27" version="1">MRDKILNKKSQYIHDLFSITDEDVVATHQLITRDISLMQLNLAEGQILQLLIKMNNIKSIIEIGTFVGSSAMCMVKALPENGYIYTIEKDTTNAELAKSNFEKYNLSHKVTVINDTAISALNKLTDLSPFDMVFIDASKAEYCNYLDWAELNIKKNGLIVADNTLLFGNVYSSDNITKKVPKTTIDKMLSFNKRLSDKNKYLTTLLPTEEGMTIALKLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSH6</accession>
    <name>SYL_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="1">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuS</name>
        <name type="ordered locus">Ecaj_0282</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304407.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSH6"/>
    <dbReference type="SMR" id="Q3YSH6"/>
    <dbReference type="STRING" id="269484.Ecaj_0282"/>
    <dbReference type="EnsemblBacteria" id="AAZ68329">
        <property type="protein sequence ID" value="AAZ68329"/>
        <property type="gene ID" value="Ecaj_0282"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0282"/>
    <dbReference type="eggNOG" id="COG0495">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004427_0_0_5"/>
    <dbReference type="OMA" id="TFMVLAP"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_B">
        <property type="entry name" value="Leu_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="PTHR43740"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000334752" description="Leucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="591"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="594"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <sequence length="829" mass="94858" checksum="B2A7FC52332711ED" modified="2005-09-27" version="1">MHYDFKKVEQDIQKKWNFYTDVKKAQCYVLEMFPYPSGNIHMGHLRNYTIGDVIARYKRACGINVFHPIGWDAFGLPAENAALSYNINPQTWTKNNIDNMRCQLKSIGLSYDWNKELATCDADYYKHEQAFFLDFLKCGLAYRKESLVNWDPIDQTVLANEQVIDGKGWRSGAVVEKRKLSQWFLKITDFAEELLNDLKVLDKWPEKVKLMQERWIGRSEGVVVDFEILNINKTLQVFTTCPHTLFGASFIAVSFDHPILQCVSDSDIIQQINDFDKNNLITDASSTVEKFGINSGLVVKHPFLCMNLPVYVVNFVLMDYATGAVFGCPAHDQRDFEFAKKYNLQIKQVVFPEIDIDLGKEAYVGSGTMKNSDFLDGMTVDEAKEAMIAKLQLLGIGDKMTYYRIHDWGISRQRYWGCPIPIIYCEKCGTVPVSRKDLPVTLPQDVDFTKSGNPLDNHPTWKYVKCPSCGMDAERETDTFDTFFESSWYFAAFCGTSSGIDKDTCNMLLPVDYYIGGIEHAVLHLLYSRFFCRALTKCGYFNVKEPFSSLITQGMVCHATYSDAQGNYLFPEEARKMMEEGQHVNVGRAEKMSKSKKNVVNLEYIIDKYGADTARLFILSDTPPDRDIEWLDDGIEGASKYLSKLWRMIVSYDQINLNFNVEDIPEDAIKYRRCVHKIVSDITSDLEFCRLNCAVAKFRELSNILSEMIRTSVNSDVVSEAICILIRVIEPFIPHIAEKLWENIGGKDMLWNQMWPKADTELLIERNVNIAVQVNGKFIRTLTVANNIDNDQLKSMALEMAKNKIGNSVVKNVYIIPKRAVNIVIEKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSK0</accession>
    <name>Q3YSK0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine triad (HIT) protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0258</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304383.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0258"/>
    <dbReference type="EnsemblBacteria" id="AAZ68305">
        <property type="protein sequence ID" value="AAZ68305"/>
        <property type="gene ID" value="Ecaj_0258"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0258"/>
    <dbReference type="eggNOG" id="COG0537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_8_1_5"/>
    <dbReference type="OMA" id="IAFMDIM"/>
    <dbReference type="OrthoDB" id="1934382at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="PTHR23089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="13"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="106"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="117" mass="13351" checksum="165D566A025DEA8F" modified="2005-09-27" version="1">MQEGNNSYDKDNVFAKILRKELPCNIVYEDESVLAFHDIHPQAPIHVLVIPKSDYISFDDFSNSSAEDIKHFFSVVKKITHKLQLDKTGYRIVTNHGKQGGQIIPHFHVHILGGKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSL2</accession>
    <name>Q3YSL2_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sec-independent protein translocase protein TatA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tatA</name>
        <name evidence="8" type="ordered locus">Ecaj_0244</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The Tat system comprises two distinct complexes: a TatABC complex, containing multiple copies of TatA, TatB and TatC subunits, and a separate TatA complex, containing only TatA subunits. Substrates initially bind to the TatABC complex, which probably triggers association of the separate TatA complex to form the active translocon.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TatA/E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304371.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0244"/>
    <dbReference type="EnsemblBacteria" id="AAZ68293">
        <property type="protein sequence ID" value="AAZ68293"/>
        <property type="gene ID" value="Ecaj_0244"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0244"/>
    <dbReference type="eggNOG" id="COG1826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_6_2_5"/>
    <dbReference type="OMA" id="WQIFLIL"/>
    <dbReference type="OrthoDB" id="1907955at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00236">
        <property type="entry name" value="TatA_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="56" mass="6278" checksum="639FB9F017F26CDD" modified="2005-09-27" version="1">MTLGPWQIFLILVIILVLFGAGRLPDVMSDLGKGIRNLKQELKDNKLASTEDEPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSM3</accession>
    <name>Q3YSM3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0233</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304360.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0233"/>
    <dbReference type="EnsemblBacteria" id="AAZ68282">
        <property type="protein sequence ID" value="AAZ68282"/>
        <property type="gene ID" value="Ecaj_0233"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0233"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004585_5_10_5"/>
    <dbReference type="OMA" id="YQDTNRT"/>
    <dbReference type="OrthoDB" id="137860at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="6"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="8">
        <location>
            <begin position="285"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="639" mass="73798" checksum="7241F79401ADA6EA" modified="2005-09-27" version="1">MEDYITSLNEDQKEAVININGPILILAGAGTGKTRTVTSRIAYIINNNFALPNQILAVTFTNKAANEMLARINEMTPAYNIWLGTFHAIATKILRLHAEIVGLKNDFTIINTDDQLQVIKNIVNDMHPEYGLDAHKIILNKIQRWKDRGLMANNITETELYKPINTVALSTYHVYQERLKFLNCVDFGDLLLYNIQILTTQHDILSHYQEQFKYIMVDEYQDINTIQYLWLRLLAQKYKNLCCVGDDDQSIYSWRGAEVGNILKFSDDFPNAKVVRLECNYRSTSNILNVATAIINNNKSRLGKKLWTTNEKGQKVNLMKFWDSKAEAQYICEYIKNSHDYQFNETAILVRAGFQTRIFEEYFIKYNIPYKIIGGTRFYDRQEIRDIIAYLKITANPNNDIAFERIINKPKRHIGASTLNKIYLCAKQNNTYFLNAAKLLVSGNQLSDKVSRSLNHLLNKIDSWKESLLSDSISNTVKMIAYDSGYIDMLENEGENGLARIENIKELFSALLSFNDVNEFLEHISLVTEMDSLDYNNNYTYIMTLHAAKGLEFPIVFLPGWEEGIFPHEKSLQDRTGEALEEERRLAYVGITRAREQLFISCAAVRDINNWRQQMKISRFIKELPAAHVQVIKNISSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSM8</accession>
    <name>Q3YSM8_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304355.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68277">
        <property type="protein sequence ID" value="AAZ68277"/>
        <property type="gene ID" value="Ecaj_0228"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0228"/>
    <dbReference type="HOGENOM" id="CLU_803478_0_0_5"/>
    <dbReference type="OMA" id="VIRVIYF"/>
    <dbReference type="OrthoDB" id="1810198at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="345" mass="39749" checksum="520BCAFB0FB0D8F1" modified="2005-09-27" version="1">MYDIITISIALTALFILFISMLIIIFLCSRKNNKIAKSEIFQQYFSSVSAEDMPKLMLSTSDDEKNDITNSDIITGTTFLTDLHRTDITIQDQQTDRNAIENMSLSYIHSQQQDQFTDIHSSYLSNINVHYAQKSNLNLSNRIHGAAILNNIFSKIDTDIAIQQNIIHELVTHFHQGGYLSTSYNIINNIFNPNIKVRYDYVIQGMNTTTSLHLINNHTITQEIENDIIVTHINDIDNTDAPKYKIKSQLTFSMLSTPNDIYSPISYKNIKLTLHLPEDMKQYVTKTKPVKRYNKNIKNAILIEKSHDKVVYQLPNFTSYNILQHCRQSLQNVQSTTVAHPYHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSN9</accession>
    <name>Q3YSN9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SMF protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DprA/Smf family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304344.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0217"/>
    <dbReference type="EnsemblBacteria" id="AAZ68266">
        <property type="protein sequence ID" value="AAZ68266"/>
        <property type="gene ID" value="Ecaj_0217"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0217"/>
    <dbReference type="eggNOG" id="COG0758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029601_0_3_5"/>
    <dbReference type="OMA" id="SEMPFGW"/>
    <dbReference type="OrthoDB" id="1881437at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009294">
        <property type="term" value="P:DNA-mediated transformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003488">
        <property type="entry name" value="DprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041614">
        <property type="entry name" value="DprA_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43022">
        <property type="entry name" value="PTHR43022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02481">
        <property type="entry name" value="DNA_processg_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17782">
        <property type="entry name" value="DprA_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00732">
        <property type="entry name" value="dprA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DprA_WH" evidence="2">
        <location>
            <begin position="323"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="374" mass="41885" checksum="66BC31AB82DE4BFB" modified="2005-09-27" version="1">MKTKKELSYQEFISCIRLIRTPNIGPSTFHALVKLYKTCQHTLEVLPNLIKKSNTNDKLYNICSIETAELEIENTNKIGGKIITVFDQDYPKALRHIHDYPPVLTVLGNTSILKEKIIGIVGSRNPSINGKNFAYKLSYELANSGFVIVSGLARGIDKSAHSIIFQQLPTIAVMASGINIVYPQENTHLYKTIIDKGGLIITEFPFATLPRAQLFPQRNRIISGLSLGIVVVEASIQSGSLITANFALEQNREVFAVPGSPLDHRCRGSNSLIKNGAKLVESTEDIIENLQFNRHNTYIQNSLFDNTVQNNYHFNEINNTKDIILQHISHSPIEIEEIITSTNLNISNILIALTELEESKKIERFPNNKVALIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSQ6</accession>
    <name>Q3YSQ6_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304327.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0199"/>
    <dbReference type="EnsemblBacteria" id="AAZ68249">
        <property type="protein sequence ID" value="AAZ68249"/>
        <property type="gene ID" value="Ecaj_0199"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0199"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1060644_0_0_5"/>
    <dbReference type="OMA" id="GICGMYV"/>
    <dbReference type="OrthoDB" id="1389691at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="132"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="261" mass="29691" checksum="24A991278B2A355F" modified="2005-09-27" version="1">MENGHISSLVVSNLKDYMIHTMQISNLEIHHFQNITLTINDSKSSLLNFAFYDNTTECNEKNIIEVLEFLKKRKIDATWPVEPHMKKLKSTLEKLGLVNVSPPKKAIANIENYIMPPIKGPSITLDIVNNEEKLLELDKSASKIFYSQQNEITTFLRGLANHHDINNSKLKFFLVKCNNIKVGICGMYVQDKVVGLYSDGVFSEYRNQGIASQMVLERIKIAKKQYNCSYAVAQCMKPSVNLYKRLGFKMLGSLSLYTSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSQ7</accession>
    <name>Q3YSQ7_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0198</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304326.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0198"/>
    <dbReference type="EnsemblBacteria" id="AAZ68248">
        <property type="protein sequence ID" value="AAZ68248"/>
        <property type="gene ID" value="Ecaj_0198"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0198"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_722880_0_0_5"/>
    <dbReference type="OMA" id="FYIWDAK"/>
    <dbReference type="OrthoDB" id="634527at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="381" mass="44985" checksum="E49843304C7FD5FA" modified="2005-09-27" version="1">MFIYITIFLLIFVLGLLINAYYRANVNICALKHNNNLINSLLSTLGDGFYLWDEKRRIERFSPNLQILLNTVFYSFDEFADFFEESKELQKKFAEAKKVNKSFTIDLKGQDTEIYCTCYGQSIVDNNGQIMGVLLWVQNITNYKLAITSLEYENTTLKENLLNYTDILNNLPYPLWKRDKDFKIKTHNSFYSELVQHNINIDIDQNPQFISKKNNTINQKVQYAIINNERKLYNMIEIPIKNSTEFIGYGEDITRLNYFTQELKNYTTLQTNLLNNLPNAVAIYNRDGSIIFYNKIFTKLWQLNSSWLDTNPNYSSILQKIYEDSNLLDKEKLDLIHQQQHEIFKQVTEPYHGTMILKDNITLKVIVIPNFKQELTFIYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSR2</accession>
    <name>Q3YSR2_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphomethylpyrimidine kinase</fullName>
            <ecNumber evidence="5">2.7.4.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ecaj_0192</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP = 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + ADP</text>
            <dbReference type="Rhea" id="RHEA:19893"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16892"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 3/3.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.7" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304321.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0192"/>
    <dbReference type="EnsemblBacteria" id="AAZ68243">
        <property type="protein sequence ID" value="AAZ68243"/>
        <property type="gene ID" value="Ecaj_0192"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0192"/>
    <dbReference type="eggNOG" id="COG0351">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020520_0_0_5"/>
    <dbReference type="OMA" id="DNRHTHG"/>
    <dbReference type="OrthoDB" id="461201at2"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00138"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="4">
        <location>
            <begin position="17"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAZ68243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="269" mass="29318" checksum="DF6493686FFA8C22" modified="2005-09-27" version="1">MTKHQYKGKVLTIAGSDSCGGAGIQADIKTISALGCYAASCITSVTAQNTSQVYNVYNMPQNIIKQQIEAVLSDIDIDAIKIGMLPSSEAIIAVAQSLPHIPIVVDPVMISTSNFRLMDNSAIFDFIKHIIYRATIITPNITEAEVLAQTKIINQDDMIKASQQIKSLGAKYVLIKGGHINQETINNILLTEDNQILNFPHQRIFDGELHGTGCTLSSAIASFISQKISVQESVNLAIQYILKTIKTVPQIGKGNNPVFHNYNVINTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSR3</accession>
    <name>Q3YSR3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304320.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0191"/>
    <dbReference type="EnsemblBacteria" id="AAZ68242">
        <property type="protein sequence ID" value="AAZ68242"/>
        <property type="gene ID" value="Ecaj_0191"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0191"/>
    <dbReference type="HOGENOM" id="CLU_658469_0_0_5"/>
    <dbReference type="OMA" id="IMNGIHN"/>
    <dbReference type="OrthoDB" id="834947at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="421" mass="46213" checksum="D730A6F9BCE105CB" modified="2005-09-27" version="1">MLGNFFSSVGRGFRAVQNYPLIVFNALRRAYSYFGVVPLLRLLPMFGTNQAVTCRARFPALHKNQTCKYVSSRCCQKGIDFYGDHVVYQGKLYPIIPLKTPDGGPVYNVQGHRLFAINTAHNNYKDFEGKIEKNKVLVVAYDGNRVVDPVQGKGSREVMLSAKHKNIYPFSFSIVNHETGQRDLVLSFLMLPLNLSGLAVGCAGRLVASVIAGVGKCCEKAAGLMSKNMDRDIIHQSSGVVTGGNKPYFRTLAVFLLSMMANSLECTASIVKNSADFSESIIRFPSGIMNGIHNGNMRCLPVTAGLAAIAQSSKSLFVGMKNSGLSLVRECVLFKARMTGDTGTLREHVGNAGLVAEQPQDVQEQQGKEEAQRKKKKMSRRDLEAVKQLGESLSSQILCGTEEDIRKELTKQRKASTKTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSR7</accession>
    <name>Q3YSR7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0187</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0187"/>
    <dbReference type="EnsemblBacteria" id="AAZ68238">
        <property type="protein sequence ID" value="AAZ68238"/>
        <property type="gene ID" value="Ecaj_0187"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0187"/>
    <dbReference type="eggNOG" id="COG0587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001600_0_0_5"/>
    <dbReference type="OMA" id="KQRFHLC"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011708">
        <property type="entry name" value="DNA_pol3_alpha_NTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041931">
        <property type="entry name" value="DNA_pol3_alpha_thumb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040982">
        <property type="entry name" value="DNA_pol3_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029460">
        <property type="entry name" value="DNAPol_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004805">
        <property type="entry name" value="PolC_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32294">
        <property type="entry name" value="PTHR32294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07733">
        <property type="entry name" value="DNA_pol3_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17657">
        <property type="entry name" value="DNA_pol3_finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14579">
        <property type="entry name" value="HHH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00594">
        <property type="entry name" value="polc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="POLIIIAc" evidence="7">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="1123" mass="127602" checksum="78C83B6A039DF380" modified="2005-09-27" version="1">MSQIFVHLRSHSDYSLLHGMVKIESLVNLCVQHNMPALALTDSGNLFGSLEFSDCASNSGIQPIIGCNIMISYNGDNIGELILLVKNQVGYSNIVNLVSNSFRNDQSGKINRIDLNTLISLKDGIIVLTGGHDGFLAQLLLGNVIDYGIIDKLLLAFKGNLYVELQRHNIEEEKLIERTLIKFAYEKDVPLVATNDILFARREDFLAHDVLSCISDGNYIAQEDRKMFTEEHYFKSSNEMHELFVDIPEAISNTVLIAQRCSFMPEIRKPMLPHFPCSSGRNESQELISQAIDGLNSRLKDSNLSKEQTSKYYERLHYELDIIISMDYAGYFLIVSDFICWSKRNDIMVGPGRGSGAGSLVAWSLQITDLDPIEFGLIFERFLNPDRISMPDFDIDFCQEGRDHVIEYVRKKYGYVAHIITFGKLQAKAVLRDVGRVMQMPYFQVDRICKMIPHNPVRPVTLSEAIEMDKNLQKERDDDETVAKLLEISLKLEGLYRHVSIHAAGIVICDRRLEELLPLYYDNVSFLPITQYNMKYTEKAGLVKFDFLGLRTLTVINQICHLVNNGGQNVDISRIPLNDKKTYEMLSAGDSVGVFQLESSGMREVISKLKPDNINDIIALISLYRPGPMDNISIYIARKHGFEKPDYIHPILEGVLKETFGVIIYQEQVMEIAKIMAGYSLGEADLLRRAMGKKIKEEMDNQRRAFISGAINNGIEEEKASYIFDLVAKFAGYGFNKSHAAAYALISYQTAYLKANYTLEFFTASMNLDITDKDKLEMLCHQAKLHGIKILPPDINTSKVFFTIEGSSSIRYALGALKNVGQHSAKEIVDDIAYKDIWDFIDRVSTKCVHKRILESIIKAGVLDSIHSNRKQLFESVFLFLDIIEYNKYNANFNQFSLFTDRAHYKLSETDDWTKEEKLDNEFSSIGFYLNHHPMENYKCLLEKLNIGFINYDDRSAYNNRIVGIISSVKVRSASRDKFAVVTLSDPYNIHEIAFYNGSVIEDNKDLFTSGAPVIIEMDNDFYSTAARLLGKNIYSFESKISSIIKSMVIYISVEDSVIIKELSSLLKDNGSTVVLINVLLPDDHVTIQLPNSFLITPLIFMQIFKLSWVRDIEINSITNEHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YST9</accession>
    <name>Q3YST9_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">50S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rplK</name>
        <name evidence="12" type="ordered locus">Ecaj_0165</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="PTM">
        <text evidence="7 9">One or more lysine residues are methylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304294.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0165"/>
    <dbReference type="EnsemblBacteria" id="AAZ68216">
        <property type="protein sequence ID" value="AAZ68216"/>
        <property type="gene ID" value="Ecaj_0165"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0165"/>
    <dbReference type="eggNOG" id="COG0080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_2_2_5"/>
    <dbReference type="OMA" id="CKQFNAK"/>
    <dbReference type="OrthoDB" id="1702697at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_L11/L12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_L11/L12_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006519">
        <property type="entry name" value="Ribosomal_L11_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_L11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_L11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_L11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_L11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="PTHR11661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="SSF46906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="SSF54747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01632">
        <property type="entry name" value="L11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L11_N" evidence="11">
        <location>
            <begin position="9"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L11" evidence="10">
        <location>
            <begin position="78"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="147" mass="15593" checksum="2A06E9B2316933D1" modified="2005-09-27" version="1">MSAVLISKINLMIEAGKANPGPKIASVLGPRGIPMPKFCKEFNDITSSEGNQYKVGDLVTARISIYNDKSYSFTISDSPVSYLLKKAAAIDKGAVNPGKDNVGKVKMSDIYEIAKRKMSDMNADTVEAAAKMVIGTASSMGIVVVEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSW9</accession>
    <name>RL35_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmI</name>
        <name type="ordered locus">Ecaj_0135</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304264.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSW9"/>
    <dbReference type="SMR" id="Q3YSW9"/>
    <dbReference type="STRING" id="269484.Ecaj_0135"/>
    <dbReference type="EnsemblBacteria" id="AAZ68186">
        <property type="protein sequence ID" value="AAZ68186"/>
        <property type="gene ID" value="Ecaj_0135"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0135"/>
    <dbReference type="eggNOG" id="COG0291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169643_2_1_5"/>
    <dbReference type="OMA" id="KQWAPYG"/>
    <dbReference type="OrthoDB" id="2046660at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00514">
        <property type="entry name" value="Ribosomal_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021137">
        <property type="entry name" value="Ribosomal_L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018265">
        <property type="entry name" value="Ribosomal_L35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001706">
        <property type="entry name" value="Ribosomal_L35_non-mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037229">
        <property type="entry name" value="Ribosomal_L35_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01632">
        <property type="entry name" value="Ribosomal_L35p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00064">
        <property type="entry name" value="RIBOSOMALL35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143034">
        <property type="entry name" value="SSF143034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00001">
        <property type="entry name" value="rpmI_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00936">
        <property type="entry name" value="RIBOSOMAL_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000258673" description="50S ribosomal protein L35">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="66" mass="7511" checksum="097E0D0FEFAD971C" modified="2005-09-27" version="1">MPKLKTKSSVKKRFSVTATGKIKSTQSAKRHGMTKRSKRSIRVQRGTAIMNPSDSRIVKLFMPYSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSX5</accession>
    <name>RS16_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsP</name>
        <name type="ordered locus">Ecaj_0129</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304258.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YSX5"/>
    <dbReference type="SMR" id="Q3YSX5"/>
    <dbReference type="STRING" id="269484.Ecaj_0129"/>
    <dbReference type="EnsemblBacteria" id="AAZ68180">
        <property type="protein sequence ID" value="AAZ68180"/>
        <property type="gene ID" value="Ecaj_0129"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0129"/>
    <dbReference type="eggNOG" id="COG0228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100590_5_0_5"/>
    <dbReference type="OMA" id="GFYNPIA"/>
    <dbReference type="OrthoDB" id="1937072at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00385">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000307">
        <property type="entry name" value="Ribosomal_S16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020592">
        <property type="entry name" value="Ribosomal_S16_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023803">
        <property type="entry name" value="Ribosomal_S16_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919">
        <property type="entry name" value="PTHR12919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00886">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54565">
        <property type="entry name" value="SSF54565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00002">
        <property type="entry name" value="S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00732">
        <property type="entry name" value="RIBOSOMAL_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000243803" description="30S ribosomal protein S16">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00385"/>
        </source>
    </evidence>
    <sequence length="87" mass="10116" checksum="D1EFD476704BF1D2" modified="2005-09-27" version="1">MSVKIRLARFGAKKRPFYRVVVADSRVSRDGKFIESLGFYNPMLPKEHELFVKVKVDRLKYWLSVGAQATDRVAWFIKKGIINIETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-04-07" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSY0</accession>
    <name>Q3YSY0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative NADH:ubiquinone oxidoreductase 17.2kD subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304253.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0124"/>
    <dbReference type="EnsemblBacteria" id="AAZ68175">
        <property type="protein sequence ID" value="AAZ68175"/>
        <property type="gene ID" value="Ecaj_0124"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0124"/>
    <dbReference type="eggNOG" id="COG3761">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110455_4_2_5"/>
    <dbReference type="OMA" id="PALWHIW"/>
    <dbReference type="OrthoDB" id="1876365at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007763">
        <property type="entry name" value="NDUFA12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12910">
        <property type="entry name" value="PTHR12910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05071">
        <property type="entry name" value="NDUFA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0830">Ubiquinone</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="99" mass="11573" checksum="A72D94512BDD82DF" modified="2005-09-27" version="1">MLFFHNLQFLIKRNKKLIGKDILGNHYYITIANGVEKRWVIYNGKADPTKIPASWHTWLHYTNNQTPKSSKSPHFPNLTGTQYAYHPNKASVFYGTKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSZ5</accession>
    <name>Q3YSZ5_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Gp36</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68160">
        <property type="protein sequence ID" value="AAZ68160"/>
        <property type="gene ID" value="Ecaj_0109"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0109"/>
    <dbReference type="HOGENOM" id="CLU_1324676_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="207" mass="22246" checksum="0943326CB0D6A64B" modified="2005-09-27" version="1">MLHLTTEITNIDFAHDFHIHQGERFGVSSGDLELDIENHPGHGYHILFKNNGHVISDLHGAKAEDFNFNMKDHSLNVSFLIDPMAPFHELDVNNHPNFFISVHAYQDGCDNCVHGNPSRPAIVNQAQVLLPSGVTEDSVSAPATEDSVSAPATEDSVSAPATEDSVSAPATEDSVSAPATAATGSTTSYNHNTGLLDLDSDILNMLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSZ7</accession>
    <name>Q3YSZ7_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integration host factor subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0107</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304236.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0107"/>
    <dbReference type="EnsemblBacteria" id="AAZ68158">
        <property type="protein sequence ID" value="AAZ68158"/>
        <property type="gene ID" value="Ecaj_0107"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0107"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_1_1_5"/>
    <dbReference type="OMA" id="IYVRGFG"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13835">
        <property type="entry name" value="IHF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005684">
        <property type="entry name" value="IHF_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="100" mass="11172" checksum="275D1229FC4D0132" modified="2005-09-27" version="1">MTQTLTKSKIAEVINKDVGLSREDAASIVGEVLDEMINALVRDHILKISSFGTFKSYKKKARMGRNPKTSEAFVIKERNTVSFYPSILVKHSINNDDGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-12-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT01</accession>
    <name>Q3YT01_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">4-diphosphocytidyl-2C-methyl-D-erythritol synthase</fullName>
            <ecNumber evidence="1">2.7.7.60</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.60" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304232.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0103"/>
    <dbReference type="EnsemblBacteria" id="AAZ68154">
        <property type="protein sequence ID" value="AAZ68154"/>
        <property type="gene ID" value="Ecaj_0103"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0103"/>
    <dbReference type="eggNOG" id="COG1211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061281_2_2_5"/>
    <dbReference type="OMA" id="TDACKIL"/>
    <dbReference type="OrthoDB" id="1836139at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050518">
        <property type="term" value="F:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02516">
        <property type="entry name" value="CDP-ME_synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034683">
        <property type="entry name" value="IspD/TarI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01128">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="231" mass="26756" checksum="336059ED5E633535" modified="2005-09-27" version="1">MYKFILLIVAAGNSSRFKWNITPKQYCLLGNHNILCSTINNMISHPKISHVKVVIRKEHQHLYSSSLPKHDKLLPPVYGGARRQDSVRIGLESLQDINPHFVIIHDACRPFVDLKLLDTMEPILNHYAGVVPVLSIIDTVHMVHEDNTVMKNIDRDTIKLVQTPQAYKYNDILLYHKTAYDIEPYNEFPDESSLMLHYNIPIMTVEGDYNNFKITTIEDLHRAILYTKYTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT19</accession>
    <name>Q3YT19_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="7">DHBP synthase</shortName>
            <ecNumber evidence="3 7">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ecaj_0085</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="3 7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304214.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0085"/>
    <dbReference type="EnsemblBacteria" id="AAZ68136">
        <property type="protein sequence ID" value="AAZ68136"/>
        <property type="gene ID" value="Ecaj_0085"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0085"/>
    <dbReference type="eggNOG" id="COG0108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_3_0_5"/>
    <dbReference type="OMA" id="DAGGLIC"/>
    <dbReference type="OrthoDB" id="900513at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAZ68136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="211" mass="23081" checksum="40F1F6E5A0697AB5" modified="2005-09-27" version="1">MGIIVSESSSFISPIEHIIEDARQGKIFILIDDEQRENEGDLVILAEKVTSDAINFMITHGSGIVCLSITEDLMCKLGLEFIPKRHVNDNSASFATSIDVRYGITTGVSAHDRASTILRVVDQNVTKDDFVTPGHIFPIVAKKGGVLERSGHTEASVEIAKIAGLAPVAVVCELMNPDGTMSRMPDIIEFSQRYNVKVTSIEKLIQYVKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT27</accession>
    <name>Q3YT27_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA polymerase III, delta subunit</fullName>
            <ecNumber evidence="3">2.7.7.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.7" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304206.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0077"/>
    <dbReference type="EnsemblBacteria" id="AAZ68128">
        <property type="protein sequence ID" value="AAZ68128"/>
        <property type="gene ID" value="Ecaj_0077"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0077"/>
    <dbReference type="eggNOG" id="COG1466">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068860_2_0_5"/>
    <dbReference type="OMA" id="WRGLHFR"/>
    <dbReference type="OrthoDB" id="1199768at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010372">
        <property type="entry name" value="DNA_pol3_delta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005790">
        <property type="entry name" value="DNA_polIII_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06144">
        <property type="entry name" value="DNA_pol3_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01128">
        <property type="entry name" value="holA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol3_delta" evidence="2">
        <location>
            <begin position="22"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="334" mass="38371" checksum="3979013F33470B97" modified="2005-09-27" version="1">MKITSSKLHSFFNNPQSFNNILIYGNDYGKINMYKEKIIKHINTNTEFSTSNIDYPTVNKNPSTLFIELTTISMFCKNKLIIIANGEKSISQELKNILNNNIGRNYVIITSGELSIDSSLRQYYDNSLNAASIGCYKDDNDNLAFIISDFLTQNKVHYNCTTLQYLCSVLSQNCNALEPELQKLMLYIGNNKNLTTKDIQESLLTEIDPISEDLCISIAEKNLENFTKFSNILLKNKFTPIALIRILIKYFLRLEYLIRVVKDGESINQALKSIHPPIFFKSIPTIKKHISTISYFEVNNIIKKLAEIEIQCKKSDLNQEVIFKYYITAMIISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-09-29" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT29</accession>
    <name>Q3YT29_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7 9">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ecaj_0075</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7 10">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304204.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0075"/>
    <dbReference type="EnsemblBacteria" id="AAZ68126">
        <property type="protein sequence ID" value="AAZ68126"/>
        <property type="gene ID" value="Ecaj_0075"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0075"/>
    <dbReference type="eggNOG" id="COG0372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025068_0_0_5"/>
    <dbReference type="OMA" id="TVGWCAQ"/>
    <dbReference type="OrthoDB" id="1412360at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00717"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004108">
        <property type="term" value="F:citrate (Si)-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06114">
        <property type="entry name" value="EcCS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024176">
        <property type="entry name" value="Citrate_synthase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010953">
        <property type="entry name" value="Citrate_synthase_typ-I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001369">
        <property type="entry name" value="Citrate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01798">
        <property type="entry name" value="cit_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" evidence="8">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001369-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="416" mass="46479" checksum="572348214410AEE8" modified="2005-09-27" version="1">MVKEAVLELDGQKVKLPVLCDTNGNKVIDITSLYKGTGILTYDPGFMSTAACESKITFIDGNKGILCYRGYNIADLVNNNKSFIEIVYLLFYGSFPSVDNLKDFMLQISQEYMPPEMVCDVIKSFSQDSHPMTILMACFSALASYYHDQAVDKDGLKHPKLAVAKVASIIAMIYRYTTNQDFIEPNHGLSYSENFIHMMFDISSYKFTQVVAKALDVIFTLHADHEQNASTATVRLAGSSGADLFACLVAGIATLWGPAHGGANEAVINMLMAIEKPSNVKQFIQKVKDGNKSTRLMGFGHRVYKNYDPRAKIICEICKEVLNDLGVNDPLLEIALELENIALQDSYFIERSLYPNVDFYSGIILKAIGIPVRMFTTLFALARTSGWSAQWYEMVADKNYKICRPRQLYTGHIAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT40</accession>
    <name>Q3YT40_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304193.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68115">
        <property type="protein sequence ID" value="AAZ68115"/>
        <property type="gene ID" value="Ecaj_0064"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0064"/>
    <dbReference type="HOGENOM" id="CLU_1218237_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021902">
        <property type="entry name" value="DUF3514"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12027">
        <property type="entry name" value="DUF3514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="227" mass="25655" checksum="5778F20FC74DE161" modified="2005-09-27" version="1">MLNKILKKFLVHVEKSQLNESIINFYFCHLLSEDDNAVYSGRFYKRVLSTLCQLVRLRLCDPDCVEQHDVLNLGIVSCAMIMGTMYKQYISLQHFSGDVKGYPSFSQVVTCMGPQRHHRGVFAQLCVTILCKICGLNNLQYNEAVLFNGLYMPGGLITNASEHFLGKITQCINNNEMFFGVVIEDLATQVCMMLDRMHLAAVKEEIEAYCAAYDKIMHSSSGQGPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT61</accession>
    <name>Q3YT61_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide deformylase</fullName>
            <shortName evidence="2">PDF</shortName>
            <ecNumber evidence="2">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">def</name>
        <name evidence="3" type="ordered locus">Ecaj_0043</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304172.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0043"/>
    <dbReference type="EnsemblBacteria" id="AAZ68094">
        <property type="protein sequence ID" value="AAZ68094"/>
        <property type="gene ID" value="Ecaj_0043"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0043"/>
    <dbReference type="eggNOG" id="COG0242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061901_2_0_5"/>
    <dbReference type="OMA" id="RAFSYNV"/>
    <dbReference type="OrthoDB" id="1649129at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00079">
        <property type="entry name" value="pept_deformyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="185" mass="21027" checksum="F7810712264186F4" modified="2005-09-27" version="1">MSVLPIVTVPDKRLSLCSEEVREVDQSVKKLVDDMFEAMHVNKGVGLAAVQVGIHKRVLVVDVPEEFRETDDITSQIEGYELCGGPYCIINPKIVDASQEKVTLREGCLSVPDYFDYVVRPQYVTVQYLDYNGNECIIKAQGWLARCLEHEIDHLNGIVFLKYLSKFKRDFAIGKVKKMRKSNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT69</accession>
    <name>Q3YT69_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH-ubiquinone oxidoreductase, putativ</fullName>
            <ecNumber evidence="3">1.6.5.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304164.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0035"/>
    <dbReference type="EnsemblBacteria" id="AAZ68086">
        <property type="protein sequence ID" value="AAZ68086"/>
        <property type="gene ID" value="Ecaj_0035"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0035"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_5_5"/>
    <dbReference type="OMA" id="FFNRFAA"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Epimerase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="320" mass="36130" checksum="30806E50DFC273BD" modified="2005-09-27" version="1">MSIRRIIIFGGSGFIGRYLVKYFAENGYIIKIFTRYPEKAKQLKLCGNLGQIEVISGDVTNVQEIENNIFGCHVVVNLLGTLYSTKNSTFYDIHAKAAENIAKAAKSCDVELMVHFSAMGIDEVQQSHYARSKLIGENLVKLAFPNAVIIRPNLVFGAEDRFFNKFAKLTMISPFLPVIGGGRAVFQPIYVDDLAKFVFYIVNNAVTDKLYNVCGPRTYSFKELLNFILSIIKRKNILINIPFSVADILACVCELKIMSIFFKLITGNTDPILTRDQVKFIRGMTESRDMYSTNSLRKAGIKCSTIENIVPKYLEIYKNF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT72</accession>
    <name>MNME_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA modification GTPase MnmE</fullName>
            <ecNumber evidence="1">3.6.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmE</name>
        <name evidence="1" type="synonym">trmE</name>
        <name type="ordered locus">Ecaj_0032</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YT72"/>
    <dbReference type="SMR" id="Q3YT72"/>
    <dbReference type="STRING" id="269484.Ecaj_0032"/>
    <dbReference type="EnsemblBacteria" id="AAZ68083">
        <property type="protein sequence ID" value="AAZ68083"/>
        <property type="gene ID" value="Ecaj_0032"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0032"/>
    <dbReference type="eggNOG" id="COG0486">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019624_3_1_5"/>
    <dbReference type="OMA" id="CEIQCHG"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00450">
        <property type="entry name" value="mnmE_trmE_thdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51709">
        <property type="entry name" value="G_TRME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000345777" description="tRNA modification GTPase MnmE">
        <location>
            <begin position="1"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="TrmE-type G">
        <location>
            <begin position="213"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="223"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="242"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="267"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00379"/>
        </source>
    </evidence>
    <sequence length="441" mass="49266" checksum="229F51BB1EE18AA9" modified="2005-09-27" version="1">MIMSTIFALCTPWGKSGVAVIRVSGKDAAKAFLHFGISSSIKPRTATFAHLYNSKGEIIDEVIIVYFVAPSSFTGEDVVEFHTHGSLAVIKMILAELGKIFVPAGPGEFSLRAFLNNKVDLTRAEAIVDLINSETEMQAKQAIRQMSGALEKLYQSWRQQLIDILSNIEAYIDFPEEVNSAALANIGYLLNNLQESLECHLNDDRKGERLRQGIYIAIVGEPNSGKSTLFNHLAKRDIAIVSEYAGTTRDTLEAHIDVAGYPIVIIDTAGIRDSADLIEQEGIRRAKLKAENADFKIVMLPYEKRNVFNNEIMNLIDEKSICVLSKADNITEHELISIFNFSFVPISVCCNRGIEILLNLIKQRVEKDFQFCSTHPFITSERQRLHIQNTLNIVKNMDLELPMEIVAEDLRLSVRELGKVVGVISDEDILDNVFGKFCIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT75</accession>
    <name>Q3YT75_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exodeoxyribonuclease 7 large subunit</fullName>
            <ecNumber evidence="5">3.1.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Exodeoxyribonuclease VII large subunit</fullName>
            <shortName evidence="5">Exonuclease VII large subunit</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">xseA</name>
        <name evidence="9" type="ordered locus">Ecaj_0029</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterooligomer composed of large and small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the XseA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.6" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304158.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0029"/>
    <dbReference type="EnsemblBacteria" id="AAZ68080">
        <property type="protein sequence ID" value="AAZ68080"/>
        <property type="gene ID" value="Ecaj_0029"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0029"/>
    <dbReference type="eggNOG" id="COG1570">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023625_2_0_5"/>
    <dbReference type="OMA" id="WPAVRFE"/>
    <dbReference type="OrthoDB" id="1371775at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04489">
        <property type="entry name" value="ExoVII_LU_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00378">
        <property type="entry name" value="Exonuc_7_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003753">
        <property type="entry name" value="Exonuc_VII_L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020579">
        <property type="entry name" value="Exonuc_VII_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025824">
        <property type="entry name" value="OB-fold_nuc-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30008">
        <property type="entry name" value="PTHR30008"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02601">
        <property type="entry name" value="Exonuc_VII_L"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13742">
        <property type="entry name" value="tRNA_anti_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00237">
        <property type="entry name" value="xseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_anti_2" evidence="8">
        <location>
            <begin position="5"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuc_VII_L" evidence="7">
        <location>
            <begin position="117"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuc_VII_L" evidence="7">
        <location>
            <begin position="320"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="388" mass="43867" checksum="0B7918AFA72B8E86" modified="2005-09-27" version="1">MIPEFTVSELTKIFQNFVHETFTHIKVRGEISNLSQPKSGHTYFTLKDDDAVLNAVCWNNTKIEFDLKNGLEVICFGFLTTYQSKYQLITESMLLAGIGNLKIMLEQRKAKLEKEGLFNQSNKKHLPLLPKIIGVITSTTGAVINDILNRVKSRFPSHIVISPVSVQGNEAINQIINAISKLNNSDINRPDVIIIARGGGSIEDLWIFNDEAIVRAVAGSSIPIVSAIGHETDFTLIDYAADIRAPTPTAAVEIVLPTKTQLVELINSRFNKIKTILHYKINIKKDRLFYLHNNLMKTKHKIKVLKLQLLEHKSQIEVLLKIMLLNKKQSLNELYYKINQFNKEKILEAGYAVIYDTNNNHINSIKKLQSNDIISIELKDGIVEAIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT81</accession>
    <name>Q3YT81_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GTP cyclohydrolase II</fullName>
            <ecNumber evidence="7">3.5.4.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ecaj_0023</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Jake</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304152.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0023"/>
    <dbReference type="EnsemblBacteria" id="AAZ68074">
        <property type="protein sequence ID" value="AAZ68074"/>
        <property type="gene ID" value="Ecaj_0023"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0023"/>
    <dbReference type="eggNOG" id="COG0807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_1_2_5"/>
    <dbReference type="OMA" id="SIDYISY"/>
    <dbReference type="OrthoDB" id="900513at2"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="SSF142695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="GTP_cyclohydro2" evidence="6">
        <location>
            <begin position="190"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAZ68074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="380" mass="43460" checksum="E1A94A20571CBC08" modified="2005-09-27" version="1">MENLFNSFRSNKYITEKSIAELRCGIPILLYNNNDSLLIFPSELANNELLSALKKKFQDINILVTGNRLNFIFQSPGNRLSRIKIKTHHDLEYISSLLIDQEFHKKSSLTADDIIVSTNSLDKTAISIIKLTKLLPSAIVIDINSANILQWCSQNNITPVKQEIIDNYNEEYEIQEVCSSPLFLKDCPNAKIKVYRSYTGELDHYAIIIGNPDYSNPIVRIHSSCYTGDLLNSLSCDCRCQLHTAIKLMTENKGGIILYLAQDGRGIGLANKIRTYQLQTKHNFDTVDANRFFGFEDDERVFTPAIKILQKLGISRLQLLTNNPNKISEIQKNNIQVTKILPIFVDTNQHNINYINTKAKRLGHVSWKCFLCYFYILMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT88</accession>
    <name>Q3YT88_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ecaj_0016</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 9">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304145.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0016"/>
    <dbReference type="EnsemblBacteria" id="AAZ68067">
        <property type="protein sequence ID" value="AAZ68067"/>
        <property type="gene ID" value="Ecaj_0016"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0016"/>
    <dbReference type="eggNOG" id="COG1146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_0_0_5"/>
    <dbReference type="OMA" id="FVTDKCI"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000813">
        <property type="entry name" value="7Fe_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022569">
        <property type="entry name" value="Fd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11953">
        <property type="entry name" value="DUF3470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00354">
        <property type="entry name" value="7FE8SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 9" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAZ68067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="125" mass="14536" checksum="02E8940A474A7DD0" modified="2005-09-27" version="1">MTHFVTDKCIRCKYTDCVEVCPVDCFYEGANMLVIDPDQCIDCGVCIPECPIDAIVPDDSIKDILECSDSELNEEQKNIKKSYEINKKFSKEWKNITSAKTAYPEAESYKYRKDKFKYFDENLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT93</accession>
    <name>Q3YT93_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CBS domain:Transporter associated domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ecaj_0011</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0053 family. Hemolysin C subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0011"/>
    <dbReference type="EnsemblBacteria" id="AAZ68062">
        <property type="protein sequence ID" value="AAZ68062"/>
        <property type="gene ID" value="Ecaj_0011"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0011"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_3_1_5"/>
    <dbReference type="OMA" id="ERRHMAL"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="136"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="295" mass="33453" checksum="8133958F699967BD" modified="2005-09-27" version="1">MSDKLSDNSDSDDRFSLSESLSVKLVSFMLKRVPKLKKIIENNLINDNEGFTNSRMFHNLIKFNDCLVRDVMIPRTEIYAIDITDVPNSKDLIDKVIRGQYTRIPAYESNLDNIIGFIHIKDIISNFHNEFNARNIIREVMYIPPSMKAVNLFIKMQSSHIHVAIVIDEYGGTEGLVTMADLIEEVVGDIGDEHDAPTVPSIVNLSDNKVEVNARILVKDLEETFNIDLRDCKEDDYVTIGGLILSMIGRVPVANEIFEHKSGVIFSIKEADDRCIYKIIIDLSKVKKIGLKVDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YTA1</accession>
    <name>Q3YTA1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="9">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glycyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="9">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glyQ</name>
        <name evidence="10" type="ordered locus">Ecaj_0003</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0003"/>
    <dbReference type="EnsemblBacteria" id="AAZ68054">
        <property type="protein sequence ID" value="AAZ68054"/>
        <property type="gene ID" value="Ecaj_0003"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0003"/>
    <dbReference type="eggNOG" id="COG0752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057066_1_0_5"/>
    <dbReference type="OMA" id="SYYQFQV"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00254">
        <property type="entry name" value="Gly_tRNA_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002310">
        <property type="entry name" value="Gly-tRNA_ligase_asu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02091">
        <property type="entry name" value="tRNA-synt_2e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01044">
        <property type="entry name" value="TRNASYNTHGA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00388">
        <property type="entry name" value="glyQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAZ68054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="280" mass="32020" checksum="1884083437EA7475" modified="2005-09-27" version="1">MNFQSIIKNLQDYWEDYGCTIFQPYTSELGAATLHPATSMTSISKKPSKLAYVQPVIRPCDGRYGDNPNRLYQHHQYQVLIKPSSTDLQLDYLKSLEKIGISIETHDIKFIEDDWENPSIGAWGLGWEVTCNGMEITQFTYMQQVGGIECSLVPGEIAYGLERIAMIIQNVDNVHKILWDDNGTTYGDVFKQREYEFSKLALDYYDVDILFKQFNEAEQVCISLIEKKLPLAAYDICTKASHILNLLESRGVIGVNERASYILRVRKLANLCCSLYTQCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YTA3</accession>
    <name>Q3YTA3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="9">2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase</fullName>
            <ecNumber evidence="9">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ecaj_0001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304130.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0001"/>
    <dbReference type="EnsemblBacteria" id="AAZ68052">
        <property type="protein sequence ID" value="AAZ68052"/>
        <property type="gene ID" value="Ecaj_0001"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0001"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_8_3_5"/>
    <dbReference type="OMA" id="AHGFNFG"/>
    <dbReference type="OrthoDB" id="867226at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018168">
        <property type="entry name" value="Ubi_Hdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01304">
        <property type="entry name" value="UBIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="8">
        <location>
            <begin position="7"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="390" mass="44448" checksum="3409BE214C527CE6" modified="2005-09-27" version="1">MNSSKYYDVVISGAGTNGIITAIALNQLSISTALIDKNKILSSMPKDRVFALSRRSQEILDKFNIWDNIKEHCPMLDILIKDEDSSIFTHYDHKSISDKPMGYIVESKFLCDAFKKHIKKLNLYSECTYKSVNIANDIVKIELTDNTTLLTSLLISAEGKNSKLRQIFDIKSINYDYFQSSIICNVKHTNHHKNLAVEHFSSVGPLAILPMYDGYRSSIVWTNKKHISEMLMKLSKQDFITELEKKCATYLNDIKLDSEIKCFPLHLTFAKHIYKNRLVLIGDAAHSLHPIAGQGLNLGIRDIDRLVDNIKSAKQYGIDIGSHYVLKNFSYDRYFDNFSMAVFTTLINNVFSRKSCIVKSARRAGLYIIQNSKSIKNYMIKHAMGVGKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQT9</accession>
    <name>Q3YQT9_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UBA/THIF-type NAD/FAD binding fold:MoeZ/MoeB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304992.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0885"/>
    <dbReference type="EnsemblBacteria" id="AAZ68916">
        <property type="protein sequence ID" value="AAZ68916"/>
        <property type="gene ID" value="Ecaj_0885"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0885"/>
    <dbReference type="eggNOG" id="COG0476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_10_0_5"/>
    <dbReference type="OMA" id="SAKWKLE"/>
    <dbReference type="OrthoDB" id="1408716at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="PTHR10953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="ThiF" evidence="2">
        <location>
            <begin position="5"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="260" mass="28725" checksum="731A281AE94EC4BC" modified="2005-09-27" version="1">MFDRYKKQILIPEIGKLGQKKLNSSHVLVVGCGGLGSVVIPLLAAAGIGHMVLCDDDTVQLSNLNRQVIYKESDINQSKVAKAKEFIRSLNSDINIQILNSFVTPKNFESIFKDIDIIVDCTDRLATKLFLNDAAVLIGKPLVHSAAIGFTGQILTIFPRGKPCLRCFFECQYMSSHLNCANAGILGPTVGVVGSIAASEVIKYLLGIEDSLVGKLQRIDLQSNDFSKYKFQKNDVCIACSDNVRVDPYDYNYYESRLCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQU1</accession>
    <name>Q3YQU1_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">zf-CHCC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68914">
        <property type="protein sequence ID" value="AAZ68914"/>
        <property type="gene ID" value="Ecaj_0884"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0884"/>
    <dbReference type="eggNOG" id="COG4391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_213749_0_0_5"/>
    <dbReference type="OMA" id="YIEHPKI"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019401">
        <property type="entry name" value="Znf_CHCC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10276">
        <property type="entry name" value="zf-CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-CHCC" evidence="1">
        <location>
            <begin position="9"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="53" mass="6035" checksum="F958D9747788591C" modified="2005-09-27" version="1">MSSDEENVRVVYCNGEGNDSDYIEHPKIYLTVKVGEEVSCPYCSKVFTFNSHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-04-07" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YQX6</accession>
    <name>Q3YQX6_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Cytochrome c oxidase assembly protein CtaG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ctaG</name>
        <name evidence="12" type="ordered locus">Ecaj_0848</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell inner membrane</location>
            <topology evidence="2 10">Single-pass type II membrane protein</topology>
            <orientation evidence="2 10">Periplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the COX11/CtaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304956.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0848"/>
    <dbReference type="EnsemblBacteria" id="AAZ68879">
        <property type="protein sequence ID" value="AAZ68879"/>
        <property type="gene ID" value="Ecaj_0848"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0848"/>
    <dbReference type="eggNOG" id="COG3175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045000_5_0_5"/>
    <dbReference type="OMA" id="NKLECFC"/>
    <dbReference type="OrthoDB" id="1821272at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008535">
        <property type="term" value="P:respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00155">
        <property type="entry name" value="CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023471">
        <property type="entry name" value="CtaG/Cox11_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007533">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_CtaG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04442">
        <property type="entry name" value="CtaG_Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005413">
        <property type="entry name" value="COX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110111">
        <property type="entry name" value="SSF110111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0186">Copper</keyword>
    <keyword evidence="9 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="10">
        <location>
            <begin position="1"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="topological domain" description="Periplasmic" evidence="10">
        <location>
            <begin position="27"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="174" mass="19939" checksum="F55F6F2FAFCDFC3D" modified="2005-09-27" version="1">MKPRIRNTIYGLIAIILSMICLVYASVPLYSIFCKVTGYGGTVRTSNISNSKIGNTIIKVRFNADIHKQLPWKFYPEVSHVFVKPGEQKLIFYRAENLLDEDTSGMAVYNVTPHKVGKYFNKVACFCFTKQTLYPHQKTIMPVSFFIDPAIETDPETADVKLITLSYVFFKYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR12</accession>
    <name>Q3YR12_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Outer membrane protein assembly factor BamD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bamD</name>
        <name evidence="3" type="ordered locus">Ecaj_0812</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the Bam complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BamD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304920.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YR12"/>
    <dbReference type="STRING" id="269484.Ecaj_0812"/>
    <dbReference type="EnsemblBacteria" id="AAZ68843">
        <property type="protein sequence ID" value="AAZ68843"/>
        <property type="gene ID" value="Ecaj_0812"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0812"/>
    <dbReference type="eggNOG" id="COG4105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065982_1_0_5"/>
    <dbReference type="OMA" id="GYQFERF"/>
    <dbReference type="OrthoDB" id="1256747at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15830">
        <property type="entry name" value="BamD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00922">
        <property type="entry name" value="OM_assembly_BamD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017689">
        <property type="entry name" value="BamD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039565">
        <property type="entry name" value="BamD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13525">
        <property type="entry name" value="YfiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03302">
        <property type="entry name" value="OM_YfiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="YfiO" evidence="2">
        <location>
            <begin position="35"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="254" mass="29615" checksum="6212D6B477A19EB0" modified="2005-09-27" version="1">MNHSKVIRNTICLLCCCIFMLGCSLVKKDIKFVEERTADEMYESALKKSGIKDYKSAVKDLEEIDNLYPFSPVAIKARLMMSFLNYELGDYSRAEIYADDYIQLYPDSKDIDFAYYLRIMANYMQISDIDRDQSSVNKVLELLDEFVRLFPNSIYLEEVMKRLDLVHQHIAAKEFSIGKFYLQRGEYVAAIKRFSTILNKYEDTKYYSESLYRIAEAYLALGDVTAYAKYMSLLKECCINTGWYKEPNLVTGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YR92</accession>
    <name>Q3YR92_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0731"/>
    <dbReference type="EnsemblBacteria" id="AAZ68763">
        <property type="protein sequence ID" value="AAZ68763"/>
        <property type="gene ID" value="Ecaj_0731"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0731"/>
    <dbReference type="HOGENOM" id="CLU_1254317_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="220" mass="24589" checksum="7E0980E1A6B00AFA" modified="2005-09-27" version="1">MLLVLLVLLVLLLILAIYFYCFRYKNQGNINALLQDKEELSLHCQVLAEKYNKLLGEYKNVILAYISEKKSSLEKAGELNDTIMSAISEALIGSLQKIHEDEGIEHDPESIIAGVVAWLTSVRIYSMQLLDGSMQMVEQSEKALAGEFSSQLSLALLSKNIRKGINTSKSKRGLSNFCAGCCACFRSQSEPEPEPEPQPQLQVQDVQVDHNNERDNARVN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRG6</accession>
    <name>BIOB_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Biotin synthase</fullName>
            <ecNumber evidence="1">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bioB</name>
        <name type="ordered locus">Ecaj_0657</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:22060"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="EC" id="2.8.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304766.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRG6"/>
    <dbReference type="SMR" id="Q3YRG6"/>
    <dbReference type="STRING" id="269484.Ecaj_0657"/>
    <dbReference type="EnsemblBacteria" id="AAZ68689">
        <property type="protein sequence ID" value="AAZ68689"/>
        <property type="gene ID" value="Ecaj_0657"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0657"/>
    <dbReference type="eggNOG" id="COG0502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033172_1_2_5"/>
    <dbReference type="OMA" id="ADRFCMG"/>
    <dbReference type="OrthoDB" id="940969at2"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="PTHR22976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001619">
        <property type="entry name" value="Biotin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00272">
        <property type="entry name" value="biotin_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000381374" description="Biotin synthase">
        <location>
            <begin position="1"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="42"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="324" mass="36442" checksum="7824C0FF36C2614B" modified="2005-09-27" version="1">MSTFTPTRNDWQLDEILELFHTAFNDLILQSHLLHRQFFSNNEVQISSLLNIKTGGCPENCKYCSQSAHYKTDLKKESLLDIESIKKAIQTAKKNGADRFCFAAAWRQVRDRDLEYICDIIDLIKSENLESCASLGMVTLEQAKKLKNAGLDFYNHNIDTSRDFYPNVTTTRSYDDRLTSLNNIHEAGINICSGGILGLGESVEDRAKMLLTLANLKEHPLSVPINRLVPIKGTPFENNAKIDNIDFIKTIAVARILMPKSYIRLAAGRMSMSEEMQALCLFAGANSIFYGEKLLTTPNADCNDDRNLLSKLGAKTKEPVFFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRK0</accession>
    <name>Q3YRK0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304732.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0621"/>
    <dbReference type="EnsemblBacteria" id="AAZ68655">
        <property type="protein sequence ID" value="AAZ68655"/>
        <property type="gene ID" value="Ecaj_0621"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0621"/>
    <dbReference type="eggNOG" id="COG3027">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133828_1_0_5"/>
    <dbReference type="OMA" id="YKISCES"/>
    <dbReference type="OrthoDB" id="1802106at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007838">
        <property type="entry name" value="Cell_div_ZapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036192">
        <property type="entry name" value="Cell_div_ZapA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042233">
        <property type="entry name" value="Cell_div_ZapA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05164">
        <property type="entry name" value="ZapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102829">
        <property type="entry name" value="SSF102829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="122" mass="14275" checksum="2219A8C1F3B4544A" modified="2005-09-27" version="1">MNNYNPQVIEITVRNNTYRVACNKGEESHLLNLVDKFNTLVNSISSAEKGKGSDALIFLLAALTLEDEKNELKVQLKKTKDEMLMYKKHFDDYLKIKNYANDLMSHILFRIEKLINKLEEYF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-11-25" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRL3</accession>
    <name>RS19_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsS</name>
        <name type="ordered locus">Ecaj_0608</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304720.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRL3"/>
    <dbReference type="SMR" id="Q3YRL3"/>
    <dbReference type="STRING" id="269484.Ecaj_0608"/>
    <dbReference type="EnsemblBacteria" id="AAZ68642">
        <property type="protein sequence ID" value="AAZ68642"/>
        <property type="gene ID" value="Ecaj_0608"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0608"/>
    <dbReference type="eggNOG" id="COG0185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144911_0_1_5"/>
    <dbReference type="OMA" id="FPEMVGH"/>
    <dbReference type="OrthoDB" id="1893906at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_S15/S19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005732">
        <property type="entry name" value="Ribosomal_S19_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01050">
        <property type="entry name" value="rpsS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000354288" description="30S ribosomal protein S19">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <sequence length="93" mass="10458" checksum="1239878294C5AB95" modified="2005-09-27" version="1">MSRSIKKPPFCAPHVLRLVNKAVAQNKLNSIINIHSRSSVILNKFIGLTFGVYNGKTYVPVKVNDNMVGRKFGEFSPTRRYTGHVGDKKVSRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRM5</accession>
    <name>RL18_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplR</name>
        <name type="ordered locus">Ecaj_0596</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304708.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YRM5"/>
    <dbReference type="SMR" id="Q3YRM5"/>
    <dbReference type="STRING" id="269484.Ecaj_0596"/>
    <dbReference type="EnsemblBacteria" id="AAZ68630">
        <property type="protein sequence ID" value="AAZ68630"/>
        <property type="gene ID" value="Ecaj_0596"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0596"/>
    <dbReference type="eggNOG" id="COG0256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098841_0_1_5"/>
    <dbReference type="OMA" id="NKQIYAQ"/>
    <dbReference type="OrthoDB" id="1816447at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_B">
        <property type="entry name" value="Ribosomal_L18_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004389">
        <property type="entry name" value="Ribosomal_L18_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="PTHR12899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00060">
        <property type="entry name" value="L18_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000251310" description="50S ribosomal protein L18">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <sequence length="121" mass="14027" checksum="0833D9DD40746B2A" modified="2005-09-27" version="1">MVSDCITRSERRKKRVRLKLRRNSSLLRLSIFKSNRHFYVQLIDDKCGRTFASASTLESEVIAVTNRKVNSNAVKIVAKLMSDRLNKLGNCKKFVFDRGPYKYTGIVSEFANELRNYGFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YRU3</accession>
    <name>Q3YRU3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-hydroxy-3-methylbut-2-enyl diphosphate reductase</fullName>
            <shortName evidence="5">HMBPP reductase</shortName>
            <ecNumber evidence="5">1.17.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ispH</name>
        <name evidence="6" type="ordered locus">Ecaj_0526</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24488"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24825"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IspH family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.4" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304640.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0526"/>
    <dbReference type="EnsemblBacteria" id="AAZ68562">
        <property type="protein sequence ID" value="AAZ68562"/>
        <property type="gene ID" value="Ecaj_0526"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0526"/>
    <dbReference type="eggNOG" id="COG0761">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027486_1_0_5"/>
    <dbReference type="OMA" id="NDSICRQ"/>
    <dbReference type="OrthoDB" id="1311145at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00097"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00105"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051745">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13944">
        <property type="entry name" value="lytB_ispH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00191">
        <property type="entry name" value="IspH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003451">
        <property type="entry name" value="LytB/IspH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30426">
        <property type="entry name" value="PTHR30426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02401">
        <property type="entry name" value="LYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00216">
        <property type="entry name" value="ispH_lytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="232"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAZ68562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="319" mass="35810" checksum="D6B95567ECCBA83F" modified="2005-09-27" version="1">MDQILKKNVEVILANPRGFCAGVSRAIEIVELAIKYHSNNRKVYVLHEIVHNKYIINSLKEMGVLFIDTLDQAEDGSILIYSAHGVSKEIEHLAQLRNLEIINATCPLVTKVHKEVQSYDREGYQIILIGHKGHREVEGTIGQITTPVLLVQTISDIDNIAVVNQNKLAYVTQTTLSVDDTKEIINKLKQKFPNIKGPDLKDICYATQNRQNAVKQLSELVDIVFILGSKNSSNSNRLKELAKLKAPAFLIDSYKEIDLSLLQNVEKIGITAGASAPEILITEVIDSLKQHLNIKLSNLEITKENIAFNIPKQLREYKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS43</accession>
    <name>TRUA_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="1">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truA</name>
        <name type="ordered locus">Ecaj_0419</name>
    </gene>
    <organism>
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304540.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3YS43"/>
    <dbReference type="SMR" id="Q3YS43"/>
    <dbReference type="STRING" id="269484.Ecaj_0419"/>
    <dbReference type="EnsemblBacteria" id="AAZ68462">
        <property type="protein sequence ID" value="AAZ68462"/>
        <property type="gene ID" value="Ecaj_0419"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0419"/>
    <dbReference type="eggNOG" id="COG0101">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014673_0_2_5"/>
    <dbReference type="OMA" id="FLYGMVR"/>
    <dbReference type="OrthoDB" id="1075262at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02570">
        <property type="entry name" value="PseudoU_synth_EcTruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PTHR11142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000017078" description="tRNA pseudouridine synthase A">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <sequence length="245" mass="28433" checksum="7696416DCBBEE48C" modified="2005-09-27" version="1">MRYKITIEYNGSNFIGWQRQKHLSNSIQEILEEAIFKFSRESVTTYVAGRTDAGVHALGQVAHFDLTADFDAYVVRNAINYHLIPHSISVLNVEKTDDEFHARFSAKKRHYLYKIINRYSPLTIDYNRAWLVRNPLDVEKMMRAVEYIKGNHDFSSFRARHCQSKSPVKTMDDLKIIHSDQCIDIYFSAISFLHNQVRIIVGTLVECGKNSFPPEYTKDILEAKDRSYAGMTAPPYGLYFVKVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS46</accession>
    <name>Q3YS46_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68459">
        <property type="protein sequence ID" value="AAZ68459"/>
        <property type="gene ID" value="Ecaj_0416"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0416"/>
    <dbReference type="HOGENOM" id="CLU_1537677_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="174" mass="19612" checksum="C65E9634D8AE4C58" modified="2005-09-27" version="1">MSLSNSLMFLFFLVVPLIVAICDIYNQGSLIKPYKTLVILMAVLLTIMYCLSYHSEKRVVFKSEGLNVTCYKVLSSLMIASGIIIPLCNLLYEVCNGLDERVTSVYKFSFFCVFSLYVISLALNYIFQNSDKNHENANDVGISSYASYGYVCNPLQSLLSQVRCHGYSEEVVHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS73</accession>
    <name>Q3YS73_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="7">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GTP cyclohydrolase I</fullName>
            <shortName evidence="7">GTP-CH-I</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">folE</name>
        <name evidence="9" type="ordered locus">Ecaj_0389</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">Jake</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homopolymer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Toroid-shaped homodecamer, composed of two pentamers of five dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="7"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304510.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0389"/>
    <dbReference type="EnsemblBacteria" id="AAZ68432">
        <property type="protein sequence ID" value="AAZ68432"/>
        <property type="gene ID" value="Ecaj_0389"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0389"/>
    <dbReference type="eggNOG" id="COG0302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049768_3_1_5"/>
    <dbReference type="OMA" id="CEHMCMS"/>
    <dbReference type="OrthoDB" id="1632367at2"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035998">
        <property type="term" value="P:7,8-dihydroneopterin 3'-triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="PTHR11109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP_cyclohydroI" evidence="8">
        <location>
            <begin position="12"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAZ68432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="190" mass="21474" checksum="DBD6DBBE32BB9CFD" modified="2005-09-27" version="1">MENGKPSDSQAEEAINLLIRWIGDDPDRVGLTGTAKRVLNIYKKFFSGYQVDMLSMQDSVLLGENYDGMIIVKNTEFTSYCEHHIVPIKGKIDIGYIPDKLIFGLGKVIKLINCFTKRLQLQERLTMEIANALDYYLSPKGVIVSIEAVHDCVACCEEIDKNNLDLQTTCVIGIFKDNIDLRKEFFTNIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YS85</accession>
    <name>Q3YS85_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage uncharacterized protein, C-terminal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304498.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0377"/>
    <dbReference type="EnsemblBacteria" id="AAZ68420">
        <property type="protein sequence ID" value="AAZ68420"/>
        <property type="gene ID" value="Ecaj_0377"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0377"/>
    <dbReference type="eggNOG" id="COG5362">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028165_1_0_5"/>
    <dbReference type="OMA" id="RYFFKHR"/>
    <dbReference type="OrthoDB" id="426360at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006517">
        <property type="entry name" value="Arc_phage_PsiM2_terminase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035421">
        <property type="entry name" value="Terminase_6C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17289">
        <property type="entry name" value="Terminase_6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01630">
        <property type="entry name" value="psiM2_ORF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terminase_6C" evidence="1">
        <location>
            <begin position="313"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="469" mass="54900" checksum="A711EAE3B4CC142B" modified="2005-09-27" version="1">MSYNFLDFLKLCFKTVSPNNKIINNWHIKIIADRLEATLNGKINRLIINMPPRFIKSICVSIAWPSWILGLNPQARIIVASYSQVLSEKLSLDNRYILQSEWYKKLFPHVILSKDQNTKQKFQTIQRGYRFATSVGGSITGEGGDILIIDDPMNPMQALSKTYRQRVCHWFEQSFMTRLNDRKNSIVIIVMHRLHHDDLTGYLLSKKLCNKWHLLSLPLIAEQNNTFYSITSPWKFKHGKKTRNILHTRKEGDPLYKKYGKKYIEELKSELGSYAFAAQYQQNPIHLSNGMIKYVWLQRYSSYNYEDTNITQSWDTASSSNKTSNYSVCTTWSYKNNCFFLLEVYKAKLEYKSLKQEIINLANHWKPNAILIEKKASGLQIVQELTNNCTIPIIGIVPTTDKVTRFYNVIPIFESRKIFLPNDAPWLSNFEQELLSFPNTQNDDQIDSITQYLHWFQNNITNIFNIREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSE0</accession>
    <name>Q3YSE0_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ecaj_0321</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304443.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0321"/>
    <dbReference type="EnsemblBacteria" id="AAZ68365">
        <property type="protein sequence ID" value="AAZ68365"/>
        <property type="gene ID" value="Ecaj_0321"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0321"/>
    <dbReference type="HOGENOM" id="CLU_182869_0_0_5"/>
    <dbReference type="OMA" id="AMYYYTW"/>
    <dbReference type="OrthoDB" id="1810382at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAZ68365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="99" mass="11866" checksum="E33DCCCBA309A7D3" modified="2005-09-27" version="1">MSFYTHDVIKKQVSDTFNKSSNSTYSVLNDPAYRDRYREKFLEAQKKGMDMVVYYDGTIALIENKIAMYQYGWHKKRREFERIKFAAETKKKKNKSNLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSG5</accession>
    <name>Q3YSG5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Membrane protein insertase YidC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Foldase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 13">Membrane integrase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Membrane protein YidC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">yidC</name>
        <name evidence="15" type="ordered locus">Ecaj_0293</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with the Sec translocase complex via SecD. Specifically interacts with transmembrane segments of nascent integral membrane proteins during membrane integration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell inner membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the OXA1/ALB3/YidC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304418.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0293"/>
    <dbReference type="EnsemblBacteria" id="AAZ68340">
        <property type="protein sequence ID" value="AAZ68340"/>
        <property type="gene ID" value="Ecaj_0293"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0293"/>
    <dbReference type="eggNOG" id="COG0706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016535_3_0_5"/>
    <dbReference type="OMA" id="INPVAGC"/>
    <dbReference type="OrthoDB" id="524437at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01810">
        <property type="entry name" value="YidC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019998">
        <property type="entry name" value="Membr_insert_YidC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="Membr_insert_YidC/Oxa1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028053">
        <property type="entry name" value="Membr_insert_YidC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038210">
        <property type="entry name" value="YidC_periplasmic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038221">
        <property type="entry name" value="YidC_periplasmic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="PTHR12428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14849">
        <property type="entry name" value="YidC_periplas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00701">
        <property type="entry name" value="60KDINNERMP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01900">
        <property type="entry name" value="YIDCPROTEIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03593">
        <property type="entry name" value="yidC_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="527"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="YidC_periplas" evidence="14">
        <location>
            <begin position="69"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAZ68340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="579" mass="66857" checksum="46125AC9FCED2007" modified="2005-09-27" version="1">MVEVRNLILAVILSVLIMTGWRFIYDKFFTTQHIIEDNTNSDTMIPEIDDSFTHKTRAEIIGSNTDIRLNLSNKKIHGSISLKGAQFDDLIFKNYHVTPDSNSDGIILLSPEETEGVYFIEFGWIDPQHKITLPNHSSIWNSDQPTLSPNNPATLTWDNKEGIIFKIRITLDNDYMFKIEQMVTNNTNSIVNLIPYGRINRSKHTTEKSYWISHEGAVGSFSNTLKEWTYKTITKEKNIRIQSQDRNDDQNQWIGIGDKYWFTALIPNVLQKSKVNFHVKHAKHNSEDRFQVDFSHDNYIAHPNKTVTSTSYLFAGAKELKLLDKYKDNLNIALFDKAVDFGVLYFITKPVFLLLEYFYDAIGNFGLAILLLTVVIKLIMFPLSYKAYVSMLKLKYLQPDILKIKELHKNDNDKMSKEISAIFKKNNVNPMSGFVPILIQIPVFFALYKVLFVTIEMRHAPFYLWIKDLSSFDTANVLTMFGLLKFNVPICIGILPVILGITMVIQQKLNNSNSLNQDKVQANFMKFLPYIFIFIFSSFPAGLIIYWICSNSITIIQQLIIKQYASKKFNFQKVVKQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSK3</accession>
    <name>Q3YSK3_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-oxoglutarate dehydrogenase E1 component</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Alpha-ketoglutarate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ecaj_0255</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">E1 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the decarboxylation of 2-oxoglutarate, the first step in the conversion of 2-oxoglutarate to succinyl-CoA and CO(2).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Part of the 2-oxoglutarate dehydrogenase (OGDH) complex composed of E1 (2-oxoglutarate dehydrogenase), E2 (dihydrolipoamide succinyltransferase) and E3 (dihydrolipoamide dehydrogenase); the complex contains multiple copies of the three enzymatic components (E1, E2 and E3).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304380.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0255"/>
    <dbReference type="EnsemblBacteria" id="AAZ68302">
        <property type="protein sequence ID" value="AAZ68302"/>
        <property type="gene ID" value="Ecaj_0255"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0255"/>
    <dbReference type="eggNOG" id="COG0567">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004709_1_0_5"/>
    <dbReference type="OMA" id="RDSYCRT"/>
    <dbReference type="OrthoDB" id="29166at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="PTHR23152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transket_pyr" evidence="11">
        <location>
            <begin position="569"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAZ68302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="912" mass="103464" checksum="63482E6A7BD8DEA3" modified="2005-09-27" version="1">MKDVTCLFTDNVDVIEDIYKRYQKDSNSVSSEWRNFFSMGLYVQENINPIDQSNSVNIIDSYNSKILELLNFFRSYGHAVADLDPLKLQVTGDLDYNKYIDLSEIKPSTKFNSVLGLDNPTLSDVIDTLKSIYCGKLGYEFMHIRNHEERLWLQNKIESVCNKISNDEKKEILQHLMEVECFEQFLHTRYPGYKRFSIEGGDSLVVAIEKIIDLSTVFNFREIVIGMSHRGRLSVLTKVMKKPYRAMIYEFKGGTAYPKDLDVSGDVKYHLGYSADRQLSTDKIVHLSLCPNPSHLESVNPVVMGKVRAKQDVLQDCDKPSIVGILVHGDASVIGQGVVAETLTLSNLTGYGICGVIHIIVNNQIGFTTNPKDSRSSFYCSDIAKLIDAPVFHVNGDSPEDIVAAIKLAVEYRQKFNKDVVIDIVCYRRYGHNEGDEPLFTQPVMYDCITKHKTPMKLYKEQLINENIITEEECKVLQTEFNNMLSEEFVQSEKYIPKQADWLKGNWTNFRRPVPGNFEDYLSDTGVDEQKLFKLAHALVNVPKEFNGNKKILKILSTRFDMVSSGENIDWATGEALAFASLLSENVRVRLSGQDCCRGTFSHRHAVLIDQVTGNSYVPLNNLGIPQANFEVFNSPLSEYAVMGFEYGYSTDSTSTLVMWEAQFGDFANGAQIIVDQFISSAETKWLRCSGLVLLLPHGYEGQGPEHSSARIERYLQLCAEDNMQVVNCTTPASYFHVLRRQICRDFRKPLVIFTPKSLLRHKMAVSKLSDFTGSFVPVIGEVYPLSSNDKVRRVVICSGKVYFDIIEARDKHKIDNIAVIRLEQYYPFPEDQLANELKHYQNAEVVWCQEEPMNMGAWGFVNSYVERVLMKINIKSKRPLCVSRPASAATAAGYASMHSNEQNDILSRVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSQ5</accession>
    <name>Q3YSQ5_EHRCJ</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylglycerophosphatase A</fullName>
            <ecNumber evidence="1">3.1.3.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphatidylglycerolphosphate phosphatase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ecaj_0200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Jake</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Lipid phosphatase which dephosphorylates phosphatidylglycerophosphate (PGP) to phosphatidylglycerol (PG).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + H2O = 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:33751"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="EC" id="3.1.3.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.27" evidence="1"/>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304328.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="STRING" id="269484.Ecaj_0200"/>
    <dbReference type="EnsemblBacteria" id="AAZ68250">
        <property type="protein sequence ID" value="AAZ68250"/>
        <property type="gene ID" value="Ecaj_0200"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0200"/>
    <dbReference type="eggNOG" id="COG1267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103734_1_1_5"/>
    <dbReference type="OMA" id="TLFFSGY"/>
    <dbReference type="OrthoDB" id="1969080at2"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00504"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006655">
        <property type="term" value="P:phosphatidylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06971">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026037">
        <property type="entry name" value="PgpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036681">
        <property type="entry name" value="PgpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007686">
        <property type="entry name" value="YutG/PgpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36305">
        <property type="entry name" value="PTHR36305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04608">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006162">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101307">
        <property type="entry name" value="SSF101307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0595">Phospholipid degradation</keyword>
    <keyword evidence="1" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="PgpA" evidence="3">
        <location>
            <begin position="6"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAZ68250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="168" mass="18902" checksum="4CADF27B92904DC0" modified="2005-09-27" version="1">MQLYMMVATWFGCGNITKAPGTVASFATVLLFPAVILSNLFGILTITLITILGCLAIPKYLLDYPNKIDPKEIVIDEVIGQSIAFSLPIFFFRYYHYIPQTFHVAYTSHYIKTFIISFIFFRIFDITKIWPINIIERISGTPGIILDDILAGIMSSICTTFIIAKIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YSV3</accession>
    <name>Q3YSV3_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Electron transport protein SCO1/SenC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0151</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCO1/2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304280.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q3YSV3"/>
    <dbReference type="STRING" id="269484.Ecaj_0151"/>
    <dbReference type="EnsemblBacteria" id="AAZ68202">
        <property type="protein sequence ID" value="AAZ68202"/>
        <property type="gene ID" value="Ecaj_0151"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0151"/>
    <dbReference type="eggNOG" id="COG1999">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050131_3_1_5"/>
    <dbReference type="OMA" id="GELKGHW"/>
    <dbReference type="OrthoDB" id="1994201at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
        <property type="entry name" value="PTHR12151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="205" mass="23170" checksum="756B50A3304C13CB" modified="2005-09-27" version="1">MKAIKFILNVCLLFAAIFLGYSYITKQGIFQTKHHDTPNTTIPNEDGIQSSFSLINQDGKTVTSQDFLGKHMLVLFGFSACKSICPAELGLVSEALAQLGNNADKLQVIFITIDPKNDTVEKLKEFHEHFDSRIQMLTGNTEDINQIIKNYKIYVGQADKDHQINHSAIMYLIDKKGSYLSHFIPDLKSQENQVDKLLSLVKQYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT41</accession>
    <name>Q3YT41_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ecaj_0063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011304192.1">
        <property type="nucleotide sequence ID" value="NC_007354.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68114">
        <property type="protein sequence ID" value="AAZ68114"/>
        <property type="gene ID" value="Ecaj_0063"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0063"/>
    <dbReference type="HOGENOM" id="CLU_391164_0_0_5"/>
    <dbReference type="OrthoDB" id="2109655at2"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004230944" evidence="2">
        <location>
            <begin position="22"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAZ68114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="705" mass="76539" checksum="9C40F6421606CE57" modified="2005-09-27" version="1">MLTMFVLSLLAVSAIFGFAAAALLESMIGGSGEEKERESKTIAESDGNQGVVKVSGRVRHRDTASDKRKKAGKNKKLKHGKYNARKSSKSGQGEKNADKVLAGTDVVSQEEDKEKHNSRKQRAGKRVGVRSSDKVDQGQDKECTTEISSDRSVKSAAKGKEVLEQKVPVEDDKSDKDVQSPGKSSKKLEQSPGKEQHLPSGGDIPLQSAGEHADIPTESKDASAVSSGVSQAFDGQDKYLALGARPKKRAEPVKSKKSEPTKKSDVQVAMAVDSDQVKKDDKSSDRSVKAGSKDKEELEKKKQSKSSKEAVIVQSANGVTADKVSQRQDEKHSAETSSSEDVESAKESKEVLEQEKSVGGDESNKGVQKDSEQSEGTKKDVQSSEKQDNASKDDIKASSDEKGGGGKPSKNVGASDLESTGDDAASGVQEQYLRQGTRPKVRASKTVKAKKHKSPKTSVEPAVQFFDWDSGTYDEKDPFLVREAAKKLSKDTKPTTGVVQPQALPDDSERKDKESGKLGIIEQSFDDTEEQLVELVKEISLGELGAAAPLESDEQSGTGTIDPDTRIAGVSAVSVLVDPHKPSKKNIFYGKASLQSFRQSQYLASSIVEYRCKVQELKEKLSVFRGMQLQYCCHMMQNMFCLVVDNKVCVNESVEGLLRNKVTDYSILVFLLKLGCLMNCAAILGKVKFLIFLLMMHNCVGRKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-09-27" modified="2020-02-26" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q3YT66</accession>
    <name>Q3YT66_EHRCJ</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ecaj_0038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ehrlichia canis (strain Jake)</name>
        <dbReference type="NCBI Taxonomy" id="269484"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Alphaproteobacteria</taxon>
            <taxon>Rickettsiales</taxon>
            <taxon>Anaplasmataceae</taxon>
            <taxon>Ehrlichia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="4015" last="4023">
            <title>The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies.</title>
            <authorList>
                <person name="Mavromatis K."/>
                <person name="Doyle C.K."/>
                <person name="Lykidis A."/>
                <person name="Ivanova N."/>
                <person name="Francino M.P."/>
                <person name="Chain P."/>
                <person name="Shin M."/>
                <person name="Malfatti S."/>
                <person name="Larimer F."/>
                <person name="Copeland A."/>
                <person name="Detter J.C."/>
                <person name="Land M."/>
                <person name="Richardson P.M."/>
                <person name="Yu X.J."/>
                <person name="Walker D.H."/>
                <person name="McBride J.W."/>
                <person name="Kyrpides N.C."/>
            </authorList>
            <dbReference type="PubMed" id="16707693"/>
            <dbReference type="DOI" id="10.1128/JB.01837-05"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Jake</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000107">
        <property type="protein sequence ID" value="AAZ68089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAZ68089">
        <property type="protein sequence ID" value="AAZ68089"/>
        <property type="gene ID" value="Ecaj_0038"/>
    </dbReference>
    <dbReference type="KEGG" id="ecn:Ecaj_0038"/>
    <dbReference type="HOGENOM" id="CLU_2584171_0_0_5"/>
    <dbReference type="Proteomes" id="UP000000435">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAZ68089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000435"/>
        </source>
    </evidence>
    <sequence length="80" mass="9321" checksum="767F3BD9ED6DA898" modified="2005-09-27" version="1">MVCLMALIYVKSMRGYLTYHTTSLILIHKWAGCGYGYMCFINVMLLLLIITILWYFMREGLLLYNLQLSLLCILSLFDAV</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>