The 1000 Genomes FTP site under goes periodic rearranges to accomodate new data sets coming in and preserve old data in a new location. If you have a particular file that you don't know if it still exists the best place to try is our current.tree file ftp://ftp.1000genomes.ebi.ac.uk/vol1/ftp/current.tree This file contains 6 columns Relative path type (directory or file) size in bytes last updated time stamp md5 You should be able to search this file with your filename and see if the file still exists and find its new location If you still can't find your file email info@1000genomes.org and the support team will be able to tell you what has happened to it.