#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-27071587	intact:EBI-25475864	-	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:tsavlq(display_short)|psi-mi:EBI-27071587(display_long)	psi-mi:"MI:0435"(protease assay)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27071585|imex:IM-29177-1	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0327"(peptide)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	-	-	chain-seq-start:3264|chain-seq-end:3569	-	figure legend:1A, 3D, 4D/E, 5A|comment:MPro at  ~~1.2 uM.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/24	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	rogid:3/VK4Ao9MyLqbilWb5z6q5VSoXo-2	rigid:x5MEIHb2xb+xDFsr/jQMJ54z2YM	false	mutation decreasing interaction rate:300-300	modified L-glutamine residue:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:17858"	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-8765046|chebi:"CHEBI:42832"|chebi:"CHEBI:416787"|chebi:"CHEBI:7840"|chebi:"CHEBI:14328"|chebi:"CHEBI:14720"|chebi:"CHEBI:24336"	intact:EBI-25475864	psi-mi:glutathione disulfid(display_short)|psi-mi:"CHEBI:17858"(display_long)|intact:Glutathione disulfide(synonym)|intact:glutathione disulphide(synonym)|intact:GSSG(synonym)|intact:oxidised glutathione(synonym)|intact:Oxidized glutathione(synonym)|intact:OXIDIZED GLUTATHIONE DISULFIDE(synonym)|intact:Oxiglutatione(synonym)|intact:"(2S,2'S)-5,5'-[disulfanediylbis({(2R)-3-[(carboxymethyl)amino]-3-oxopropane-1,2-diyl}imino)]bis(2-amino-5-oxopentanoic acid)"(iupac name)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0435"(protease assay)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27071601|imex:IM-29177-21	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0586"(inhibitor)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	-	inchi key:YPZRWBKMTBYPTK-BJDJZHNGSA-N	chain-seq-start:3264|chain-seq-end:3569	figure legend:1A, 5A|comment:"MPro at 1.2 uM (mainly monomeric)."|comment:"GSH had little effect at this concentration. When  Mpro concentration was increased to 18 uM (mainly dimeric form), it was resistant to GSSG inhibition."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/24	-	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	-	false	-	mutation with no effect:300-300	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:17858"	intact:EBI-27071587	intact:EBI-8765046|chebi:"CHEBI:42832"|chebi:"CHEBI:416787"|chebi:"CHEBI:7840"|chebi:"CHEBI:14328"|chebi:"CHEBI:14720"|chebi:"CHEBI:24336"	-	psi-mi:glutathione disulfid(display_short)|psi-mi:"CHEBI:17858"(display_long)|intact:Glutathione disulfide(synonym)|intact:glutathione disulphide(synonym)|intact:GSSG(synonym)|intact:oxidised glutathione(synonym)|intact:Oxidized glutathione(synonym)|intact:OXIDIZED GLUTATHIONE DISULFIDE(synonym)|intact:Oxiglutatione(synonym)|intact:"(2S,2'S)-5,5'-[disulfanediylbis({(2R)-3-[(carboxymethyl)amino]-3-oxopropane-1,2-diyl}imino)]bis(2-amino-5-oxopentanoic acid)"(iupac name)	psi-mi:tsavlq(display_short)|psi-mi:EBI-27071587(display_long)	psi-mi:"MI:0435"(protease assay)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27071601|imex:IM-29177-21	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0586"(inhibitor)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0327"(peptide)	-	-	-	inchi key:YPZRWBKMTBYPTK-BJDJZHNGSA-N	-	figure legend:1A, 5A|comment:"MPro at 1.2 uM (mainly monomeric)."|comment:"GSH had little effect at this concentration. When  Mpro concentration was increased to 18 uM (mainly dimeric form), it was resistant to GSSG inhibition."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/24	-	rogid:3/VK4Ao9MyLqbilWb5z6q5VSoXo-2	-	false	-	modified L-glutamine residue:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:42170"	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-27072766|chebi:"CHEBI:42166"|chebi:"CHEBI:32886"	intact:EBI-25475864	psi-mi:d-1,4-dithiothreitol(display_short)|psi-mi:"CHEBI:42170"(display_long)|intact:"(2S,3S)-1,4-dimercaptobutane-2,3-diol"(synonym)|intact:D-DTT(synonym)|intact:D-threo-1,4-dimercapto-2,3-butanediol(synonym)|intact:"(2S,3S)-1,4-disulfanylbutane-2,3-diol"(iupac name)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0435"(protease assay)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072774|imex:IM-29177-23	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	-	-	chain-seq-start:3264|chain-seq-end:3569	figure legend:1G, 5B|comment:Glutathionylated Mpro had less than 5% of the activity of unmodified Mpro; following the addition of 10 mM DTT, the activity was fully restored.|comment:Mpro at 1uM.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/25	-	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	-	false	-	mutation with no effect:300-300|disulfide conjugated residue:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:42170"	intact:EBI-27071587	intact:EBI-27072766|chebi:"CHEBI:42166"|chebi:"CHEBI:32886"	-	psi-mi:d-1,4-dithiothreitol(display_short)|psi-mi:"CHEBI:42170"(display_long)|intact:"(2S,3S)-1,4-dimercaptobutane-2,3-diol"(synonym)|intact:D-DTT(synonym)|intact:D-threo-1,4-dimercapto-2,3-butanediol(synonym)|intact:"(2S,3S)-1,4-disulfanylbutane-2,3-diol"(iupac name)	psi-mi:tsavlq(display_short)|psi-mi:EBI-27071587(display_long)	psi-mi:"MI:0435"(protease assay)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072774|imex:IM-29177-23	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0327"(peptide)	-	-	-	-	-	figure legend:1G, 5B|comment:Glutathionylated Mpro had less than 5% of the activity of unmodified Mpro; following the addition of 10 mM DTT, the activity was fully restored.|comment:Mpro at 1uM.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/25	-	rogid:3/VK4Ao9MyLqbilWb5z6q5VSoXo-2	-	false	-	modified L-glutamine residue:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:63213"	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-27072835	intact:EBI-25475864	psi-mi:tcep(display_short)|psi-mi:"CHEBI:63213"(display_long)|intact:3,3',3''-phosphinidynetrispropanoic acid(synonym)|intact:"tris(2-carboxyethyl)phosphine"(synonym)|intact:"3,3',3\"-phosphanetriyltripropanoic acid"(iupac name)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0435"(protease assay)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072843|imex:IM-29177-13	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	-	-	chain-seq-start:3264|chain-seq-end:3569	figure legend:3D|comment:Treatment with TCEP to remove the glutathione moiety, resulted in a significant increase in activity.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/25	-	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	-	false	-	disulfide conjugated residue:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-27071587	intact:EBI-25475864	-	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:tsavlq(display_short)|psi-mi:EBI-27071587(display_long)	psi-mi:"MI:0435"(protease assay)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072843|imex:IM-29177-13	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0327"(peptide)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	-	-	chain-seq-start:3264|chain-seq-end:3569	-	figure legend:3D|comment:Treatment with TCEP to remove the glutathione moiety, resulted in a significant increase in activity.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/25	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	rogid:3/VK4Ao9MyLqbilWb5z6q5VSoXo-2	-	false	disulfide conjugated residue:?-?	modified L-glutamine residue:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:16856"	uniprotkb:P35754	intact:EBI-10824516|chebi:"CHEBI:42873"|chebi:"CHEBI:43049"|chebi:"CHEBI:5437"|chebi:"CHEBI:12402"|chebi:"CHEBI:14327"|chebi:"CHEBI:24334"	intact:EBI-3905236|uniprotkb:Q3KQS1|uniprotkb:B2R4L2|uniprotkb:Q6ICT1|ensembl:ENSP00000237858|ensembl:ENSP00000369314|ensembl:ENSP00000422708|ensembl:ENSP00000424636|ensembl:ENSP00000427353	psi-mi:glutathione(display_short)|psi-mi:"CHEBI:16856"(display_long)|intact:gamma-L-Glutamyl-L-cysteinyl-glycine(synonym)|intact:5-L-Glutamyl-L-cysteinylglycine(synonym)|intact:Glutathione(synonym)|intact:Glutathione-SH(synonym)|intact:GSH(synonym)|intact:"N-(N-gamma-L-Glutamyl-L-cysteinyl)glycine"(synonym)|intact:Reduced glutathione(synonym)|intact:L-gamma-glutamyl-L-cysteinylglycine(iupac name)	psi-mi:glrx1_human(display_long)|uniprotkb:GLRX(gene name)|psi-mi:GLRX(display_short)|uniprotkb:GRX(gene name synonym)|uniprotkb:Thioltransferase-1(gene name synonym)	psi-mi:"MI:0435"(protease assay)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072957|imex:IM-29177-27	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0682"(cofactor)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:NP_001112362.1|refseq:NP_001230587.1|refseq:NP_001230588.1|refseq:NP_002055.1|ensembl:ENSG00000173221(gene)|ensembl:ENST00000237858(transcript)|ensembl:ENST00000379979(transcript)|ensembl:ENST00000505427(transcript)|ensembl:ENST00000508780(transcript)|ensembl:ENST00000512469(transcript)|go:"GO:0004362"("glutathione-disulfide reductase (NADPH) activity")|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0015038"(glutathione disulfide oxidoreductase activity)|go:"GO:0015949"(nucleobase-containing small molecule interconversion)|go:"GO:0045838"(positive regulation of membrane potential)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0070062"(extracellular exosome)|go:"GO:0080058"(protein deglutathionylation)|go:"GO:0097573"(glutathione oxidoreductase activity)|go:"GO:2000651"(positive regulation of sodium ion transmembrane transporter activity)|interpro:IPR002109(Glutaredoxin)|interpro:IPR011767(Glutaredoxin active site)|interpro:IPR011899(Glutaredoxin, eukaryotic and viruses)|interpro:IPR014025(Glutaredoxin subgroup)|interpro:IPR036249|rcsb pdb:1B4Q|rcsb pdb:1JHB|rcsb pdb:4RQR|reactome:R-HSA-499943	-	-	-	figure legend:4 D/E, Supp. figure 4E|comment:Incubation of glutathionylated Mpro with  Grx in the presence of GSH led to a significant increase in protease activity.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/26	-	rogid:TuN1okhO+Dk2jJYF8F8l4I9jqZQ9606	-	false	-	flag tag:?-?|myc tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P35754	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-3905236|uniprotkb:Q3KQS1|uniprotkb:B2R4L2|uniprotkb:Q6ICT1|ensembl:ENSP00000237858|ensembl:ENSP00000369314|ensembl:ENSP00000422708|ensembl:ENSP00000424636|ensembl:ENSP00000427353	intact:EBI-25475864	psi-mi:glrx1_human(display_long)|uniprotkb:GLRX(gene name)|psi-mi:GLRX(display_short)|uniprotkb:GRX(gene name synonym)|uniprotkb:Thioltransferase-1(gene name synonym)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0435"(protease assay)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072957|imex:IM-29177-27	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001112362.1|refseq:NP_001230587.1|refseq:NP_001230588.1|refseq:NP_002055.1|ensembl:ENSG00000173221(gene)|ensembl:ENST00000237858(transcript)|ensembl:ENST00000379979(transcript)|ensembl:ENST00000505427(transcript)|ensembl:ENST00000508780(transcript)|ensembl:ENST00000512469(transcript)|go:"GO:0004362"("glutathione-disulfide reductase (NADPH) activity")|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0015038"(glutathione disulfide oxidoreductase activity)|go:"GO:0015949"(nucleobase-containing small molecule interconversion)|go:"GO:0045838"(positive regulation of membrane potential)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0070062"(extracellular exosome)|go:"GO:0080058"(protein deglutathionylation)|go:"GO:0097573"(glutathione oxidoreductase activity)|go:"GO:2000651"(positive regulation of sodium ion transmembrane transporter activity)|interpro:IPR002109(Glutaredoxin)|interpro:IPR011767(Glutaredoxin active site)|interpro:IPR011899(Glutaredoxin, eukaryotic and viruses)|interpro:IPR014025(Glutaredoxin subgroup)|interpro:IPR036249|rcsb pdb:1B4Q|rcsb pdb:1JHB|rcsb pdb:4RQR|reactome:R-HSA-499943	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	-	-	chain-seq-start:3264|chain-seq-end:3569	figure legend:4 D/E, Supp. figure 4E|comment:Incubation of glutathionylated Mpro with  Grx in the presence of GSH led to a significant increase in protease activity.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/26	rogid:TuN1okhO+Dk2jJYF8F8l4I9jqZQ9606	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	-	false	flag tag:?-?|myc tag:?-?	disulfide conjugated residue:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P35754	intact:EBI-27071587	intact:EBI-3905236|uniprotkb:Q3KQS1|uniprotkb:B2R4L2|uniprotkb:Q6ICT1|ensembl:ENSP00000237858|ensembl:ENSP00000369314|ensembl:ENSP00000422708|ensembl:ENSP00000424636|ensembl:ENSP00000427353	-	psi-mi:glrx1_human(display_long)|uniprotkb:GLRX(gene name)|psi-mi:GLRX(display_short)|uniprotkb:GRX(gene name synonym)|uniprotkb:Thioltransferase-1(gene name synonym)	psi-mi:tsavlq(display_short)|psi-mi:EBI-27071587(display_long)	psi-mi:"MI:0435"(protease assay)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072957|imex:IM-29177-27	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0327"(peptide)	refseq:NP_001112362.1|refseq:NP_001230587.1|refseq:NP_001230588.1|refseq:NP_002055.1|ensembl:ENSG00000173221(gene)|ensembl:ENST00000237858(transcript)|ensembl:ENST00000379979(transcript)|ensembl:ENST00000505427(transcript)|ensembl:ENST00000508780(transcript)|ensembl:ENST00000512469(transcript)|go:"GO:0004362"("glutathione-disulfide reductase (NADPH) activity")|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0015038"(glutathione disulfide oxidoreductase activity)|go:"GO:0015949"(nucleobase-containing small molecule interconversion)|go:"GO:0045838"(positive regulation of membrane potential)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0070062"(extracellular exosome)|go:"GO:0080058"(protein deglutathionylation)|go:"GO:0097573"(glutathione oxidoreductase activity)|go:"GO:2000651"(positive regulation of sodium ion transmembrane transporter activity)|interpro:IPR002109(Glutaredoxin)|interpro:IPR011767(Glutaredoxin active site)|interpro:IPR011899(Glutaredoxin, eukaryotic and viruses)|interpro:IPR014025(Glutaredoxin subgroup)|interpro:IPR036249|rcsb pdb:1B4Q|rcsb pdb:1JHB|rcsb pdb:4RQR|reactome:R-HSA-499943	-	-	-	-	figure legend:4 D/E, Supp. figure 4E|comment:Incubation of glutathionylated Mpro with  Grx in the presence of GSH led to a significant increase in protease activity.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/26	rogid:TuN1okhO+Dk2jJYF8F8l4I9jqZQ9606	rogid:3/VK4Ao9MyLqbilWb5z6q5VSoXo-2	-	false	flag tag:?-?|myc tag:?-?	modified L-glutamine residue:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:17858"	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-8765046|chebi:"CHEBI:42832"|chebi:"CHEBI:416787"|chebi:"CHEBI:7840"|chebi:"CHEBI:14328"|chebi:"CHEBI:14720"|chebi:"CHEBI:24336"	intact:EBI-25475864	psi-mi:glutathione disulfid(display_short)|psi-mi:"CHEBI:17858"(display_long)|intact:Glutathione disulfide(synonym)|intact:glutathione disulphide(synonym)|intact:GSSG(synonym)|intact:oxidised glutathione(synonym)|intact:Oxidized glutathione(synonym)|intact:OXIDIZED GLUTATHIONE DISULFIDE(synonym)|intact:Oxiglutatione(synonym)|intact:"(2S,2'S)-5,5'-[disulfanediylbis({(2R)-3-[(carboxymethyl)amino]-3-oxopropane-1,2-diyl}imino)]bis(2-amino-5-oxopentanoic acid)"(iupac name)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0943"(detection by mass spectrometry)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0945"(oxidoreductase activity electron transfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27071622|imex:IM-29177-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	go:"GO:0010731"(protein glutathionylation)	inchi key:YPZRWBKMTBYPTK-BJDJZHNGSA-N	chain-seq-start:3264|chain-seq-end:3569	figure legend:1C-E, Supp. figure 7|comment:"GSSG glutathionylates  SARS-CoV-2 Mpro at low Mpro  concentrations when Mpro is largely monomeric.  Treatment of 1.2 uM Mpro with 2 mM GSSG led to ~ 45% monoglutathionylation; treatment with 10 mM GSSG led to mono- (11%), di- (50%), and triglutathionylation (35%), with less than 4% of Mpro remaining unmodified."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/25	-	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	-	false	-	mutation decreasing interaction strength:300-300|protein modification:?-?	-	-	psi-mi:"MI:0661"(experimental participant identification)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:42170"	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-27072766|chebi:"CHEBI:42166"|chebi:"CHEBI:32886"	intact:EBI-25475864	psi-mi:d-1,4-dithiothreitol(display_short)|psi-mi:"CHEBI:42170"(display_long)|intact:"(2S,3S)-1,4-dimercaptobutane-2,3-diol"(synonym)|intact:D-DTT(synonym)|intact:D-threo-1,4-dimercapto-2,3-butanediol(synonym)|intact:"(2S,3S)-1,4-disulfanylbutane-2,3-diol"(iupac name)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0943"(detection by mass spectrometry)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0945"(oxidoreductase activity electron transfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072757|imex:IM-29177-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	go:"GO:0080058"(protein deglutathionylation)	-	chain-seq-start:3264|chain-seq-end:3569	figure legend:1F|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/25	-	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	-	false	-	disulfide conjugated residue:?-?	-	-	psi-mi:"MI:0661"(experimental participant identification)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:63213"	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-27072835	intact:EBI-25475864	psi-mi:tcep(display_short)|psi-mi:"CHEBI:63213"(display_long)|intact:3,3',3''-phosphinidynetrispropanoic acid(synonym)|intact:"tris(2-carboxyethyl)phosphine"(synonym)|intact:"3,3',3\"-phosphanetriyltripropanoic acid"(iupac name)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0943"(detection by mass spectrometry)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0945"(oxidoreductase activity electron transfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072973|imex:IM-29177-17	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	go:"GO:0080058"(protein deglutathionylation)	-	chain-seq-start:3264|chain-seq-end:3569	figure legend:Table 1,2 Figure S6|comment:Peptide digests containing glutathionylated forms of mpro were treated with  TCEP to remove disulfide-bound glutathione.  Subsequently,  masses for predicted glutathionylated peptides were no longer detected.|comment:The majority of the monoglutathionylation was occurring at Cys300.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/26	-	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	-	false	-	disulfide conjugated residue:?-?|modified L-cysteine residue:300-300|modified L-cysteine residue:156-156	-	-	psi-mi:"MI:0661"(experimental participant identification)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:16856"	chebi:"CHEBI:16240"	intact:EBI-10824516|chebi:"CHEBI:42873"|chebi:"CHEBI:43049"|chebi:"CHEBI:5437"|chebi:"CHEBI:12402"|chebi:"CHEBI:14327"|chebi:"CHEBI:24334"	intact:EBI-6927689|chebi:"CHEBI:13354"|chebi:"CHEBI:13355"|chebi:"CHEBI:295106"|chebi:"CHEBI:24637"|chebi:"CHEBI:44812"|chebi:"CHEBI:5586"	psi-mi:glutathione(display_short)|psi-mi:"CHEBI:16856"(display_long)|intact:gamma-L-Glutamyl-L-cysteinyl-glycine(synonym)|intact:5-L-Glutamyl-L-cysteinylglycine(synonym)|intact:Glutathione(synonym)|intact:Glutathione-SH(synonym)|intact:GSH(synonym)|intact:"N-(N-gamma-L-Glutamyl-L-cysteinyl)glycine"(synonym)|intact:Reduced glutathione(synonym)|intact:L-gamma-glutamyl-L-cysteinylglycine(iupac name)	psi-mi:hydrogen peroxide(display_short)|psi-mi:"CHEBI:16240"(display_long)|intact:"[OH(OH)]"(synonym)|intact:dihydrogen dioxide(synonym)|intact:H2O2(synonym)|intact:HOOH(synonym)|intact:Hydrogen peroxide(synonym)|intact:HYDROGEN PEROXIDE(synonym)|intact:Oxydol(synonym)|intact:"bis(hydridooxygen)(O--O)"(iupac name)|intact:dihydrogen peroxide(iupac name)|intact:"dihydrogen(peroxide)"(iupac name)|intact:dioxidane(iupac name)|intact:hydrogen peroxide(iupac name)	psi-mi:"MI:0943"(detection by mass spectrometry)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0945"(oxidoreductase activity electron transfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072995|imex:IM-29177-19	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0579"(electron donor)	psi-mi:"MI:0580"(electron acceptor)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0328"(small molecule)	-	-	go:"GO:0010731"(protein glutathionylation)	-	inchi key:MHAJPDPJQMAIIY-UHFFFAOYSA-N	figure legend:Supp. figure 8|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/26	-	-	-	false	-	-	-	-	psi-mi:"MI:0661"(experimental participant identification)	psi-mi:"MI:0661"(experimental participant identification)
chebi:"CHEBI:16856"	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-10824516|chebi:"CHEBI:42873"|chebi:"CHEBI:43049"|chebi:"CHEBI:5437"|chebi:"CHEBI:12402"|chebi:"CHEBI:14327"|chebi:"CHEBI:24334"	intact:EBI-25475864	psi-mi:glutathione(display_short)|psi-mi:"CHEBI:16856"(display_long)|intact:gamma-L-Glutamyl-L-cysteinyl-glycine(synonym)|intact:5-L-Glutamyl-L-cysteinylglycine(synonym)|intact:Glutathione(synonym)|intact:Glutathione-SH(synonym)|intact:GSH(synonym)|intact:"N-(N-gamma-L-Glutamyl-L-cysteinyl)glycine"(synonym)|intact:Reduced glutathione(synonym)|intact:L-gamma-glutamyl-L-cysteinylglycine(iupac name)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0943"(detection by mass spectrometry)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0945"(oxidoreductase activity electron transfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072995|imex:IM-29177-19	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0579"(electron donor)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	go:"GO:0010731"(protein glutathionylation)	-	chain-seq-start:3264|chain-seq-end:3569	figure legend:Supp. figure 8|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/26	-	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	-	false	-	mutation decreasing interaction strength:300-300|modified L-cysteine residue:?-?	-	-	psi-mi:"MI:0661"(experimental participant identification)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0DTD1-PRO_0000449623	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-25475864	intact:EBI-25475864	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0071"(molecular sieving)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072811|imex:IM-29177-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	-	chain-seq-start:3264|chain-seq-end:3569	chain-seq-start:3264|chain-seq-end:3569	figure legend:2A-F, 3 A-C, Supp. figure 3|comment:"Native Mpro was in equilibrium between monomeric and dimeric forms: glutathionylated Mpro behaved  as a monomer."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/25	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	rigid:x8zjUh7Hu9Bss8jPnZX6S6ox/y8	false	-	-	2	0	psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry)
chebi:"CHEBI:63213"	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-27072835	intact:EBI-25475864	psi-mi:tcep(display_short)|psi-mi:"CHEBI:63213"(display_long)|intact:3,3',3''-phosphinidynetrispropanoic acid(synonym)|intact:"tris(2-carboxyethyl)phosphine"(synonym)|intact:"3,3',3\"-phosphanetriyltripropanoic acid"(iupac name)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0071"(molecular sieving)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0945"(oxidoreductase activity electron transfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072829|imex:IM-29177-25	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	go:"GO:0080058"(protein deglutathionylation)	-	chain-seq-start:3264|chain-seq-end:3569	figure legend:Supp. figure 3|comment:TCEP  removes the glutathione moiety causing an increase in the dimer peak and decrease in the monomer peak.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/25	-	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	-	false	-	disulfide conjugated residue:?-?	-	-	psi-mi:"MI:0661"(experimental participant identification)	psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:P0DTD1-PRO_0000449623	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-25475864	intact:EBI-25475864	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0028"(cosedimentation in solution)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072815|imex:IM-29177-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	-	chain-seq-start:3264|chain-seq-end:3569	chain-seq-start:3264|chain-seq-end:3569	figure legend:2G|comment:"Average M. wt =63kDa (dimeric)"|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:2.4x10^-6(molar)	2021/08/19	2021/08/25	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	rigid:x8zjUh7Hu9Bss8jPnZX6S6ox/y8	false	-	-	2	0	psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:P0DTD1-PRO_0000449623	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-25475864	intact:EBI-25475864	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0028"(cosedimentation in solution)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072820|imex:IM-29177-11	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	-	chain-seq-start:3264|chain-seq-end:3569	chain-seq-start:3264|chain-seq-end:3569	figure legend:2H|comment:"Glutathionylated Mpro behaved almost completely as a monomer:Average M. Wt = 37kDa."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:200.0x10^-6(molar)	2021/08/19	2021/08/25	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	rigid:x8zjUh7Hu9Bss8jPnZX6S6ox/y8	false	disulfide conjugated residue:?-?	disulfide conjugated residue:?-?	2	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:16856"	uniprotkb:P35754	intact:EBI-10824516|chebi:"CHEBI:42873"|chebi:"CHEBI:43049"|chebi:"CHEBI:5437"|chebi:"CHEBI:12402"|chebi:"CHEBI:14327"|chebi:"CHEBI:24334"	intact:EBI-3905236|uniprotkb:Q3KQS1|uniprotkb:B2R4L2|uniprotkb:Q6ICT1|ensembl:ENSP00000237858|ensembl:ENSP00000369314|ensembl:ENSP00000422708|ensembl:ENSP00000424636|ensembl:ENSP00000427353	psi-mi:glutathione(display_short)|psi-mi:"CHEBI:16856"(display_long)|intact:gamma-L-Glutamyl-L-cysteinyl-glycine(synonym)|intact:5-L-Glutamyl-L-cysteinylglycine(synonym)|intact:Glutathione(synonym)|intact:Glutathione-SH(synonym)|intact:GSH(synonym)|intact:"N-(N-gamma-L-Glutamyl-L-cysteinyl)glycine"(synonym)|intact:Reduced glutathione(synonym)|intact:L-gamma-glutamyl-L-cysteinylglycine(iupac name)	psi-mi:glrx1_human(display_long)|uniprotkb:GLRX(gene name)|psi-mi:GLRX(display_short)|uniprotkb:GRX(gene name synonym)|uniprotkb:Thioltransferase-1(gene name synonym)	psi-mi:"MI:0979"(oxidoreductase assay)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0945"(oxidoreductase activity electron transfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072856|imex:IM-29177-15	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0682"(cofactor)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:NP_001112362.1|refseq:NP_001230587.1|refseq:NP_001230588.1|refseq:NP_002055.1|ensembl:ENSG00000173221(gene)|ensembl:ENST00000237858(transcript)|ensembl:ENST00000379979(transcript)|ensembl:ENST00000505427(transcript)|ensembl:ENST00000508780(transcript)|ensembl:ENST00000512469(transcript)|go:"GO:0004362"("glutathione-disulfide reductase (NADPH) activity")|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0015038"(glutathione disulfide oxidoreductase activity)|go:"GO:0015949"(nucleobase-containing small molecule interconversion)|go:"GO:0045838"(positive regulation of membrane potential)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0070062"(extracellular exosome)|go:"GO:0080058"(protein deglutathionylation)|go:"GO:0097573"(glutathione oxidoreductase activity)|go:"GO:2000651"(positive regulation of sodium ion transmembrane transporter activity)|interpro:IPR002109(Glutaredoxin)|interpro:IPR011767(Glutaredoxin active site)|interpro:IPR011899(Glutaredoxin, eukaryotic and viruses)|interpro:IPR014025(Glutaredoxin subgroup)|interpro:IPR036249|rcsb pdb:1B4Q|rcsb pdb:1JHB|rcsb pdb:4RQR|reactome:R-HSA-499943	go:"GO:0080058"(protein deglutathionylation)|go:"GO:0015038"(glutathione disulfide oxidoreductase activity)	-	-	figure legend:4 A-C, Supp. figure 4 A-D|comment:Incubation of glutathionylated Mpro with Grx and  GSH resulted a decrease in  glutathionylated Mpro.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/25	-	rogid:TuN1okhO+Dk2jJYF8F8l4I9jqZQ9606	-	false	-	flag tag:?-?|myc tag:?-?	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:P35754	uniprotkb:P0DTD1-PRO_0000449623	intact:EBI-3905236|uniprotkb:Q3KQS1|uniprotkb:B2R4L2|uniprotkb:Q6ICT1|ensembl:ENSP00000237858|ensembl:ENSP00000369314|ensembl:ENSP00000422708|ensembl:ENSP00000424636|ensembl:ENSP00000427353	intact:EBI-25475864	psi-mi:glrx1_human(display_long)|uniprotkb:GLRX(gene name)|psi-mi:GLRX(display_short)|uniprotkb:GRX(gene name synonym)|uniprotkb:Thioltransferase-1(gene name synonym)	psi-mi:p0dtd1-pro_0000449623(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)	psi-mi:"MI:0979"(oxidoreductase assay)	Davis et al. (2021)	pubmed:34399606|imex:IM-29177	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2)	psi-mi:"MI:0945"(oxidoreductase activity electron transfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-27072856|imex:IM-29177-15	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001112362.1|refseq:NP_001230587.1|refseq:NP_001230588.1|refseq:NP_002055.1|ensembl:ENSG00000173221(gene)|ensembl:ENST00000237858(transcript)|ensembl:ENST00000379979(transcript)|ensembl:ENST00000505427(transcript)|ensembl:ENST00000508780(transcript)|ensembl:ENST00000512469(transcript)|go:"GO:0004362"("glutathione-disulfide reductase (NADPH) activity")|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0015038"(glutathione disulfide oxidoreductase activity)|go:"GO:0015949"(nucleobase-containing small molecule interconversion)|go:"GO:0045838"(positive regulation of membrane potential)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0070062"(extracellular exosome)|go:"GO:0080058"(protein deglutathionylation)|go:"GO:0097573"(glutathione oxidoreductase activity)|go:"GO:2000651"(positive regulation of sodium ion transmembrane transporter activity)|interpro:IPR002109(Glutaredoxin)|interpro:IPR011767(Glutaredoxin active site)|interpro:IPR011899(Glutaredoxin, eukaryotic and viruses)|interpro:IPR014025(Glutaredoxin subgroup)|interpro:IPR036249|rcsb pdb:1B4Q|rcsb pdb:1JHB|rcsb pdb:4RQR|reactome:R-HSA-499943	intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent)	go:"GO:0080058"(protein deglutathionylation)|go:"GO:0015038"(glutathione disulfide oxidoreductase activity)	-	chain-seq-start:3264|chain-seq-end:3569	figure legend:4 A-C, Supp. figure 4 A-D|comment:Incubation of glutathionylated Mpro with Grx and  GSH resulted a decrease in  glutathionylated Mpro.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/08/19	2021/08/25	rogid:TuN1okhO+Dk2jJYF8F8l4I9jqZQ9606	rogid:9u1TJXDTORfCyqj2bv2TlDMZ65c2697049	-	false	flag tag:?-?|myc tag:?-?	disulfide conjugated residue:?-?	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0396"(predetermined participant)|psi-mi:"MI:0427"(Identification by mass spectrometry)