#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P0DTC9 uniprotkb:P0DTC9 intact:EBI-25475856 intact:EBI-25475856 psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:0426"(light microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902460|imex:IM-28855-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - - - figure legend:1B|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rigid:MzG5DiZK8DCqQNvq2HeenkdxQ+E false his tag:n-n|enhanced green fluorescent protein tag:n-n his tag:n-n|enhanced green fluorescent protein tag:n-n - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0DTC9 uniprotkb:P0DTC9 intact:EBI-25475856 intact:EBI-25475856 psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:0426"(light microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902472|imex:IM-28855-41 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - - - figure legend:1C|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rigid:MzG5DiZK8DCqQNvq2HeenkdxQ+E false his tag:n-n|alexa 546 label:?-? his tag:n-n|alexa 546 label:?-? - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0DTC9 uniprotkb:P0DTC9 intact:EBI-25475856 intact:EBI-25475856 psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902523|imex:IM-28855-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - - - figure legend:"1E, (Supp. figure 4)"|comment:SARS-CoV-2 N can partially freely diffuse within the condensed phase.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rigid:MzG5DiZK8DCqQNvq2HeenkdxQ+E false his tag:n-n|enhanced green fluorescent protein tag:n-n his tag:n-n|enhanced green fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTC9 intact:EBI-26902561 intact:EBI-25475856 - psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902572|imex:IM-28855-43 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0320"(ribonucleic acid) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 refseq:NC_045512.2(see-also) - - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 figure legend:1K, 2C, Supp. figure 4|comment:Addition of PARP Inhibitors CVL218 or PJ34 led to larger sizes and faster recovery rates of the fluorescence intensity of the SARS-CoV-2 N-RNA condensates than the DMSO group, in vitro.|antagonist:"CVL218 (CHEBI:167899)"|antagonist:"PJ34 (CHEBI:167900)"|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - - false his tag:n-n|enhanced green fluorescent protein tag:n-n - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) chebi:"CHEBI:167899" intact:EBI-26902561 intact:EBI-26902625 - psi-mi:cvl218(display_short)|psi-mi:"CHEBI:167899"(display_long) psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902754|imex:IM-28855-29 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0586"(inhibitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 figure legend:2C|comment:Addition of PARP Inhibitors CVL218 or PJ34 led to larger sizes and faster recovery rates of the fluorescence intensity of the SARS-CoV-2 N-RNA condensates than the DMSO group, in vitro.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - - - false - - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTC9 - intact:EBI-25475856 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902754|imex:IM-28855-29 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 - figure legend:2C|comment:Addition of PARP Inhibitors CVL218 or PJ34 led to larger sizes and faster recovery rates of the fluorescence intensity of the SARS-CoV-2 N-RNA condensates than the DMSO group, in vitro.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - false - his tag:n-n|enhanced green fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTC9 - intact:EBI-25475856 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902766|imex:IM-28855-45 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 - figure legend:2C|comment:Addition of PARP Inhibitors CVL218 or PJ34 led to larger sizes and faster recovery rates of the fluorescence intensity of the SARS-CoV-2 N-RNA condensates than the DMSO group, in vitro.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - false - his tag:n-n|enhanced green fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) chebi:"CHEBI:167900" intact:EBI-26902561 intact:EBI-26902661 - psi-mi:pj34(display_short)|psi-mi:"CHEBI:167900"(display_long) psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902766|imex:IM-28855-45 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0586"(inhibitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 figure legend:2C|comment:Addition of PARP Inhibitors CVL218 or PJ34 led to larger sizes and faster recovery rates of the fluorescence intensity of the SARS-CoV-2 N-RNA condensates than the DMSO group, in vitro.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - - - false - - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTC9 - intact:EBI-25475856 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26902935|imex:IM-28855-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 - figure legend:2 K/L|comment:Fluorescence recovery rates of SARS-CoV-2 N-RNA-nsp12 condensates were faster in the PARP inhibitor -CVL218 or PJ34- treated group than those of the control treatment.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - false cy5 label:1-1 his tag:n-n|enhanced green fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTD1-PRO_0000449629 - intact:EBI-25475885 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:p0dtd1-pro_0000449629(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26902935|imex:IM-28855-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 chain-seq-start:4393|chain-seq-end:5324 figure legend:2 K/L|comment:Fluorescence recovery rates of SARS-CoV-2 N-RNA-nsp12 condensates were faster in the PARP inhibitor -CVL218 or PJ34- treated group than those of the control treatment.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - rogid:kbHtZYssQIRdvLv8DVwmVD7XSW02697049 - false cy5 label:1-1 alexa 546 label:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) chebi:"CHEBI:167899" intact:EBI-26902561 intact:EBI-26902625 - psi-mi:cvl218(display_short)|psi-mi:"CHEBI:167899"(display_long) psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26902953|imex:IM-28855-13 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0586"(inhibitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2341"(luminescence transmitter) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 figure legend:2 K/L|comment:Fluorescence recovery rates of SARS-CoV-2 N-RNA-nsp12 condensates were faster in the PARP inhibitor -CVL218 or PJ34- treated group than those of the control treatment.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - - - false - cy5 label:1-1 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTC9 - intact:EBI-25475856 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26902953|imex:IM-28855-13 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 - figure legend:2 K/L|comment:Fluorescence recovery rates of SARS-CoV-2 N-RNA-nsp12 condensates were faster in the PARP inhibitor -CVL218 or PJ34- treated group than those of the control treatment.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - false cy5 label:1-1 his tag:n-n|enhanced green fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTD1-PRO_0000449629 - intact:EBI-25475885 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:p0dtd1-pro_0000449629(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26902953|imex:IM-28855-13 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 chain-seq-start:4393|chain-seq-end:5324 figure legend:2 K/L|comment:Fluorescence recovery rates of SARS-CoV-2 N-RNA-nsp12 condensates were faster in the PARP inhibitor -CVL218 or PJ34- treated group than those of the control treatment.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - rogid:kbHtZYssQIRdvLv8DVwmVD7XSW02697049 - false cy5 label:1-1 alexa 546 label:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTC9 - intact:EBI-25475856 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26902968|imex:IM-28855-51 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 - figure legend:2 K/L|comment:Fluorescence recovery rates of SARS-CoV-2 N-RNA-nsp12 condensates were faster in the PARP inhibitor -CVL218 or PJ34- treated group than those of the control treatment.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - false cy5 label:1-1 his tag:n-n|enhanced green fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTD1-PRO_0000449629 - intact:EBI-25475885 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:p0dtd1-pro_0000449629(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26902968|imex:IM-28855-51 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 chain-seq-start:4393|chain-seq-end:5324 figure legend:2 K/L|comment:Fluorescence recovery rates of SARS-CoV-2 N-RNA-nsp12 condensates were faster in the PARP inhibitor -CVL218 or PJ34- treated group than those of the control treatment.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - rogid:kbHtZYssQIRdvLv8DVwmVD7XSW02697049 - false cy5 label:1-1 alexa 546 label:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) chebi:"CHEBI:167900" intact:EBI-26902561 intact:EBI-26902661 - psi-mi:pj34(display_short)|psi-mi:"CHEBI:167900"(display_long) psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26902968|imex:IM-28855-51 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0586"(inhibitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:2341"(luminescence transmitter) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0320"(ribonucleic acid) - refseq:NC_045512.2(see-also) - - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 figure legend:2 K/L|comment:Fluorescence recovery rates of SARS-CoV-2 N-RNA-nsp12 condensates were faster in the PARP inhibitor -CVL218 or PJ34- treated group than those of the control treatment.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - - - false - cy5 label:1-1 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTC9 uniprotkb:P0DTC9 intact:EBI-25475856 intact:EBI-25475856 psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:1016"(fluorescence recovery after photobleaching) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26902536|imex:IM-28855-25 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - - - figure legend:1F-H|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9534(chlae-vero_e6)|taxid:9534(chlae-vero_e6 cells) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rigid:MzG5DiZK8DCqQNvq2HeenkdxQ+E false mcherry fluorescent protein tag:n-n mcherry fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTC9 uniprotkb:P0DTC9 intact:EBI-25475856 intact:EBI-25475856 psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902509|imex:IM-28855-23 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - - - figure legend:1C|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rigid:MzG5DiZK8DCqQNvq2HeenkdxQ+E false his tag:n-n|alexa 546 label:?-? his tag:n-n|alexa 546 label:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTC9 uniprotkb:P0DTC9 intact:EBI-25475856 intact:EBI-25475856 psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902497|imex:IM-28855-21 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - - - figure legend:1 B/D/I, Supp. figure 3|comment:SARS-CoV-2 N condensates fused and coalesced into larger ones at their intersections. Figure 1B -25 umol/L mEGFP tagged SARS-CoV-2 N protein. Figure 1D- 50 umol/L mEGFP-N Figure 1I- 375 nmol/L mEGFP-N|antagonist:Sodium chloride concentration >200mM|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rigid:MzG5DiZK8DCqQNvq2HeenkdxQ+E false his tag:n-n|enhanced green fluorescent protein tag:n-n his tag:n-n|enhanced green fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTC9 intact:EBI-26902561 intact:EBI-25475856 - psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902582|imex:IM-28855-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0320"(ribonucleic acid) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 refseq:NC_045512.2(see-also) - - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 figure legend:1I, Supp. figure 5C|comment:All truncations of the N protein retained LLPS ability, though more weakly than the full-length protein.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - - false his tag:n-n|enhanced green fluorescent protein tag:n-n cy5 label:1-1 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTC9 intact:EBI-26902561 intact:EBI-25475856 - psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902595|imex:IM-28855-27 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0320"(ribonucleic acid) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 refseq:NC_045512.2(see-also) - - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 figure legend:1N|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - - false mutation disrupting interaction strength:197-197|mutation decreasing interaction strength:203-203|his tag:n-n|alexa 546 label:?-?|mutation decreasing interaction strength:194-194|mutation decreasing interaction strength:188-188 - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTC9 - intact:EBI-25475856 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26902796|imex:IM-28855-47 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 - figure legend:2F-I|comment:"In the presence or absence of nsp12, the sizes of SARS-CoV-2 N-RNA condensates treated with PARP inhibitor CVL218 (CHEBI:167899) or PJ34 (CHEBI:167900) were significantly larger than those of the DMSO treated group."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - false cy5 label:1-1 his tag:n-n|enhanced green fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTD1-PRO_0000449629 - intact:EBI-25475885 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:p0dtd1-pro_0000449629(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26902796|imex:IM-28855-47 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 chain-seq-start:4393|chain-seq-end:5324 figure legend:2F-I|comment:"In the presence or absence of nsp12, the sizes of SARS-CoV-2 N-RNA condensates treated with PARP inhibitor CVL218 (CHEBI:167899) or PJ34 (CHEBI:167900) were significantly larger than those of the DMSO treated group."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 - rogid:kbHtZYssQIRdvLv8DVwmVD7XSW02697049 - false cy5 label:1-1 alexa 546 label:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTD1-PRO_0000449629 uniprotkb:P0DTC9 intact:EBI-25475885 intact:EBI-25475856 psi-mi:p0dtd1-pro_0000449629(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26902904|imex:IM-28855-49 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - chain-seq-start:4393|chain-seq-end:5324 - figure legend:2F-I|comment:"In the presence or absence of nsp12, the sizes of SARS-CoV-2 N-RNA condensates treated with PARP inhibitor CVL218 (CHEBI:167899) or PJ34 (CHEBI:167900) were significantly larger than those of the DMSO treated group."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:kbHtZYssQIRdvLv8DVwmVD7XSW02697049 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rigid:7FLCCuEKl8U9r3sE35JbHuavdbU false alexa 546 label:?-? his tag:n-n|enhanced green fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTC9 intact:EBI-26903104 intact:EBI-25475856 - psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:human beta-actin rna oligo(display_short)|psi-mi:EBI-26903104(display_long) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26903090|imex:IM-28855-15 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0320"(ribonucleic acid) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 refseq:NM_001101.5(see-also) - - comment:range 311-340 figure legend:Supp. figure 5A|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - - false his tag:n-n|enhanced green fluorescent protein tag:n-n cy5 label:1-1 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTC9 intact:EBI-26903152 intact:EBI-25475856 - psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:ds 30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26903152(display_long) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26903132|imex:IM-28855-33 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:2359"(ds rna) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 refseq:NC_045512.2(see-also) - - - figure legend:Supp. figure 5A|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - - false his tag:n-n|enhanced green fluorescent protein tag:n-n cy5 label:1-1 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTC9 intact:EBI-26903190 intact:EBI-25475856 refseq:NC_045512.2 psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:30-bp ssdna viral oligo,orf8 region(display_short)|psi-mi:EBI-26903190(display_long) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26903170|imex:IM-28855-53 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0680"(single stranded deoxyribonucleic acid) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - - - - figure legend:Supp. figure 5A|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - - false his tag:n-n|enhanced green fluorescent protein tag:n-n cy5 label:1-1 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTD1-PRO_0000449629 intact:EBI-26902561 intact:EBI-25475885 - psi-mi:p0dtd1-pro_0000449629(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26903343|imex:IM-28855-55 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0320"(ribonucleic acid) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) refseq:NC_045512.2(see-also) - chain-seq-start:4393|chain-seq-end:5324 comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 figure legend:Supp. figure 8A|comment:nsp12 readily converted to amorphous condensates when mixed with viral RNA.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/07 rogid:kbHtZYssQIRdvLv8DVwmVD7XSW02697049 - - false alexa 546 label:?-? cy5 label:1-1 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTC9 - intact:EBI-25475856 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26903750|imex:IM-28855-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 - figure legend:Supp. figure 9C|comment:The RdRp complex can be readily recruited into the SARS-CoV-2-N-RNA condensates.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/08 - rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - false cy5 label:1-1 his tag:n-n|enhanced green fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 wwpdb:7ctt - intact:EBI-25564960|wwpdb:6xqb|emdb:EMD-22288|wwpdb:6M71|wwpdb:7btf|emdb:EMD-30178|emdb:EMD-30127|intenz:2.7.7.48|wwpdb:7bv2|emdb:EMD-30210|wwpdb:7bv1|emdb:EMD-30209|wwpdb:6x2g|wwpdb:7bw4|emdb:EMD-30226|wwpdb:7l1f|emdb:EMD-23109|wwpdb:6yyt|emdb:EMD-11007|wwpdb:7c2k|emdb:EMD-30275|wwpdb:7bzf|emdb:EMD-30252|reactome:R-COV-9691364|wwpdb:7aap|emdb:EMD-11692|wwpdb:7d4f|emdb:EMD-30572|emdb:EMD-23009|wwpdb:7krp|wwpdb:7b3b|emdb:EMD-11993|wwpdb:7b3c|emdb:EMD-11994|wwpdb:7b3d|emdb:EMD-11995|rhea:"RHEA:21248"|wwpdb:7ozv|emdb:EMD-13138|wwpdb:7ozu|emdb:EMD-13135|wwpdb:7oyg|emdb:EMD-13116 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:nsp7-nsp8-nsp12_sars2(display_short)|psi-mi:7ctt(display_long)|intact:SARS-CoV-2 polymerase complex(complex recommended name)|intact:"NSP7:2xNSP8:NSP12"(complex systematic name)|intact:replication-transcription complex(complex synonym)|intact:replication and transcription complex(complex synonym)|intact:RTC(complex synonym)|intact:central RTC(complex synonym)|intact:C-RTC(complex synonym)|intact:holo-RdRp complex(complex synonym)|intact:holo-RNA-dependent RNA polymerase complex(complex synonym) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26903750|imex:IM-28855-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) refseq:NC_045512.2(see-also) intact:EBI-25506373(exp-evidence)|pubmed:32277040(see-also)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0039694"(viral RNA genome replication)|efo:"EFO:0000694"(see-also)|pubmed:16228002(see-also)|go:"GO:0031381"(viral RNA-directed RNA polymerase complex)|pubmed:19875418(see-also)|pubmed:31138817(see-also)|pubmed:31987001(see-also)|pubmed:18255185(see-also)|complex portal:CPX-5742(complex-primary)|pubmed:32015508(see-also)|evidence ontology:"ECO:0000353" - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 curated-complex:"RNA-directed 5'-3' RNA polymerase of the SARS-CoV-2 coronavirus which consists of the main polymerase protein NSP12 and a stoichiometric variant of the primase complex (CPX-5690). Extends partially double-stranded RNA templates and is probably also required for transcription initiation, though the mechanism for this has yet to be determined. Complex formation enhances dsRNA binding of the individual protomers."|complex-assembly:Heterotetramer|disease:"Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death."|complex-properties:"Primase complex without polymerase has a different stoichiometry (8:8). NSP12 possesses conserved motifs involved in template binding, NTP binding, and polymerization that assume a structure resembling a cupped “right hand”. The NSP7-NSP8 heterodimer binds to the index finger loop and may facilitates the interaction of NSP12 with additional components of the RNA synthesis machinery. The second NSP8 molecule binds close to the RNA template-binding channel." figure legend:Supp. figure 9C|comment:The RdRp complex can be readily recruited into the SARS-CoV-2-N-RNA condensates.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/08 - - - false cy5 label:1-1 his tag:c-c|alexa 546 label:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTC9 - intact:EBI-25475856 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26903818|imex:IM-28855-37 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 - figure legend:Supp. figure 10C|comment:"The ubiquitin-like domain 1 (Ubl1) of nsp3 interacting with N protein was also verified to colocalize with the condensates of SARS-CoV-2 N-RNA complex."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/08 - rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - false cy5 label:1-1 his tag:n-n|enhanced green fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26902561 uniprotkb:P0DTD1-PRO_0000449621 - intact:EBI-25492388 psi-mi:30-bp rna viral oligo, orf8 region(display_short)|psi-mi:EBI-26902561(display_long) psi-mi:p0dtd1-pro_0000449621(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26903818|imex:IM-28855-37 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_045512.2(see-also) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - comment:residues 28248-28277 from NC_045512.2|comment:residues 28248-28277 chain-seq-start:819|chain-seq-end:2763 figure legend:Supp. figure 10C|comment:"The ubiquitin-like domain 1 (Ubl1) of nsp3 interacting with N protein was also verified to colocalize with the condensates of SARS-CoV-2 N-RNA complex."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/08 - rogid:edNmSGWH7aKPoOZtWjGjL1M0aK42697049 - false cy5 label:1-1 alexa 546 label:?-?|sufficient binding region:1-112 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) chebi:"CHEBI:167899" uniprotkb:P0DTC9 intact:EBI-26902625 intact:EBI-25475856 psi-mi:cvl218(display_short)|psi-mi:"CHEBI:167899"(display_long) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:0107"(surface plasmon resonance) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902623|imex:IM-28855-7 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0326"(protein) - rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - - - figure legend:2A|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kd:4.7x10^-6(molar) 2021/03/29 2021/04/07 - rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - false - his tag:n-n - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) chebi:"CHEBI:167900" uniprotkb:P0DTC9 intact:EBI-26902661 intact:EBI-25475856 psi-mi:pj34(display_short)|psi-mi:"CHEBI:167900"(display_long) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:0107"(surface plasmon resonance) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26902654|imex:IM-28855-9 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0326"(protein) - rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - - - figure legend:2B|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kd:696.0x10^-6(molar) 2021/03/29 2021/04/07 - rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 - false - his tag:n-n - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0DTC9 uniprotkb:P0DTC9 intact:EBI-25475856 intact:EBI-25475856 psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0469"(IntAct) intact:EBI-26902786|imex:IM-28855-31 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - - - figure legend:2D, Supp. figure 7|comment:"Both PARP inhibitor CVL218 (CHEBI:167899) and PJ34 (CHEBI:167900) treatments resulted in a greater number of of puncta, but no change in the occupied area."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9534(chlae-vero_e6)|taxid:9534(chlae-vero_e6 cells) - 2021/03/29 2021/04/07 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rigid:MzG5DiZK8DCqQNvq2HeenkdxQ+E false mcherry fluorescent protein tag:n-n mcherry fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTD1-PRO_0000449629 uniprotkb:P0DTD1-PRO_0000449629 intact:EBI-25475885 intact:EBI-25475885 psi-mi:p0dtd1-pro_0000449629(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0dtd1-pro_0000449629(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0469"(IntAct) intact:EBI-26903382|imex:IM-28855-17 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) go:"GO:0005737"(cytoplasm) chain-seq-start:4393|chain-seq-end:5324 chain-seq-start:4393|chain-seq-end:5324 figure legend:Supp. figure 8B|comment:Overexpressed GFP-nsp12 in Vero E6 cells aggregated into asymmetric condensates of dense structure in the cytoplasm.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9534(chlae-vero_e6)|taxid:9534(chlae-vero_e6 cells) - 2021/03/29 2021/04/07 rogid:kbHtZYssQIRdvLv8DVwmVD7XSW02697049 rogid:kbHtZYssQIRdvLv8DVwmVD7XSW02697049 rigid:W4Bo6JkEzYRa+SXNHx34ApxXVBU false green fluorescent protein tag:n-n green fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTD1-PRO_0000449629 uniprotkb:P0DTC9 intact:EBI-25475885 intact:EBI-25475856 psi-mi:p0dtd1-pro_0000449629(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:ncap_sars2(display_long)|uniprotkb:N(gene name)|psi-mi:N(display_short)|uniprotkb:Nucleocapsid protein(gene name synonym) psi-mi:"MI:0663"(confocal microscopy) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0469"(IntAct) intact:EBI-26903853|imex:IM-28855-39 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) rcsb pdb:7CR5|rcsb pdb:7LGD|rcsb pdb:7LUX|rcsb pdb:7LUZ|rcsb pdb:7LV2|rcsb pdb:7N0I|rcsb pdb:7N0R|rcsb pdb:7R98|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0019013"(viral nucleocapsid)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044177"(host cell Golgi apparatus)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|rcsb pdb:6M3M|rcsb pdb:6WJI|rcsb pdb:6WKP|rcsb pdb:6YI3|rcsb pdb:6YUN|rcsb pdb:7C22|refseq:YP_009724397.2|interpro:IPR043505|interpro:IPR044344|interpro:IPR044345|interpro:IPR001218(Coronavirus nucleocapsid protein)|interpro:IPR037179|interpro:IPR037195|rcsb pdb:7DE1|rcsb pdb:7KGO|rcsb pdb:7KGP|rcsb pdb:7KGQ|rcsb pdb:7KGR|rcsb pdb:7KGS|rcsb pdb:7KGT|rcsb pdb:6VYO|rcsb pdb:6WZO|rcsb pdb:6WZQ|rcsb pdb:6ZCO|rcsb pdb:7CDZ|rcsb pdb:7CE0|rcsb pdb:7ACS|rcsb pdb:7ACT|reactome:R-HSA-9694322|reactome:R-HSA-9694614|reactome:R-HSA-9694631|reactome:R-HSA-9694635|reactome:R-HSA-9694686|reactome:R-HSA-9694786 - chain-seq-start:4393|chain-seq-end:5324 - figure legend:Supp. figure 11|comment:"CVL218 (CHEBI:167899) or PJ34 (CHEBI:167900) treatment enhances the co-localization of SARS-CoV-2 N and nsp12."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9534(chlae-vero_e6)|taxid:9534(chlae-vero_e6 cells) - 2021/03/29 2021/04/08 rogid:kbHtZYssQIRdvLv8DVwmVD7XSW02697049 rogid:duUiCXyS98OXMbfCdv4gp2blWT42697049 rigid:7FLCCuEKl8U9r3sE35JbHuavdbU false green fluorescent protein tag:n-n mcherry fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTD1-PRO_0000449626 uniprotkb:P0DTD1-PRO_0000449625 intact:EBI-25475874 intact:EBI-25475871 psi-mi:p0dtd1-pro_0000449626(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0dtd1-pro_0000449625(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0071"(molecular sieving) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26903686|imex:IM-28855-35 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - chain-seq-start:3943|chain-seq-end:4140 chain-seq-start:3860|chain-seq-end:3942 figure legend:Supp. figure 9 A/B|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2021/03/29 2021/04/08 rogid:Bxev1BKtZTT1Fyf+lpsPapVosnc2697049 rogid:3AF/ThDr7adqPPYFWsiWQE2FVyY2697049 rigid:9bglx2taGFlx9F8JoF9EfOktY+Y false - - - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0DTD1-PRO_0000449625 uniprotkb:P0DTD1-PRO_0000449626 intact:EBI-25475871 intact:EBI-25475874 psi-mi:p0dtd1-pro_0000449625(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0dtd1-pro_0000449626(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0071"(molecular sieving) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26903721|imex:IM-28855-57 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - chain-seq-start:3860|chain-seq-end:3942 chain-seq-start:3943|chain-seq-end:4140 figure legend:Supp. figure 9 A/B|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/08 rogid:3AF/ThDr7adqPPYFWsiWQE2FVyY2697049 rogid:Bxev1BKtZTT1Fyf+lpsPapVosnc2697049 rigid:TdMBDIvOL7GW+tgRvY3boc7Q6tU false - - - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0DTD1-PRO_0000449625 uniprotkb:P0DTD1-PRO_0000449629 intact:EBI-25475871 intact:EBI-25475885 psi-mi:p0dtd1-pro_0000449625(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0dtd1-pro_0000449629(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0071"(molecular sieving) Zhao et al. (2021) pubmed:33770364|imex:IM-28855 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26903721|imex:IM-28855-57 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - chain-seq-start:3860|chain-seq-end:3942 chain-seq-start:4393|chain-seq-end:5324 figure legend:Supp. figure 9 A/B|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/03/29 2021/04/08 rogid:3AF/ThDr7adqPPYFWsiWQE2FVyY2697049 rogid:kbHtZYssQIRdvLv8DVwmVD7XSW02697049 rigid:TdMBDIvOL7GW+tgRvY3boc7Q6tU false - his tag:c-c - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant)