#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26618043|imex:IM-28745-21 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. figure 2 C/D, 3D|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/09 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false - cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618200|imex:IM-28745-41 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0941"(competitor) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. figure 2 E/F|comment:"NSP1(KH/AA mutant) was unable to block the NSP1-40S subunit interaction, whereas either wild-type NSP1 or NSP1(RK/AA) prevented comigration of labeled NSP1 with human 40S subunits."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/09 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false - mutation disrupting interaction strength:164-165|mutation with no effect:124-125 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618200|imex:IM-28745-41 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. figure 2 E/F|comment:"NSP1(KH/AA mutant) was unable to block the NSP1-40S subunit interaction, whereas either wild-type NSP1 or NSP1(RK/AA) prevented comigration of labeled NSP1 with human 40S subunits."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/09 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false - cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P41567 intact:EBI-25401791 intact:EBI-726200|intact:EBI-1059306|uniprotkb:Q9UNQ9|ensembl:ENSP00000419449 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:eif1_human(display_long)|uniprotkb:Protein translation factor SUI1 homolog(gene name synonym)|uniprotkb:Sui1iso1(gene name synonym)|uniprotkb:A121(gene name synonym)|uniprotkb:EIF1(gene name)|psi-mi:EIF1(display_short)|uniprotkb:SUI1(gene name synonym) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618332|imex:IM-28745-23 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0840"(stimulator) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) refseq:NP_005792.1|ensembl:ENSG00000173812(gene)|ensembl:ENST00000469257(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006446"(regulation of translational initiation)|go:"GO:0008135"(translation factor activity, RNA binding)|go:"GO:0016282"(eukaryotic 43S preinitiation complex)|go:"GO:0043024"(ribosomal small subunit binding)|interpro:IPR001950(Translation initiation factor SUI1)|interpro:IPR005874(Eukaryotic translation initiation factor SUI1)|interpro:IPR036877|rcsb pdb:2IF1|rcsb pdb:4KZX|rcsb pdb:4KZY|rcsb pdb:6YBW|rcsb pdb:6ZMW|rcsb pdb:6ZP4|rcsb pdb:6ZVJ|rcsb pdb:7A09|dip:DIP-50783N - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:Supp. figure 2 F/G/I, 3D|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/09 - rogid:NVdVREA4Yjtxp30eKQr0GUvlYhg9606 - false - - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618332|imex:IM-28745-23 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. figure 2 F/G/I, 3D|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/09 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false - cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P47813 intact:EBI-25401791 intact:EBI-1045377|uniprotkb:Q0VGC2|uniprotkb:Q5JPS5|uniprotkb:Q5JPS6|uniprotkb:B2R5U5|ensembl:ENSP00000368927 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:if1ax_human(display_long)|uniprotkb:Eukaryotic translation initiation factor 4C(gene name synonym)|uniprotkb:EIF1AX(gene name)|psi-mi:EIF1AX(display_short)|uniprotkb:EIF1A(gene name synonym)|uniprotkb:EIF4C(gene name synonym) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618387|imex:IM-28745-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) refseq:NP_001403.1|ensembl:ENSG00000173674(gene)|ensembl:ENST00000379607(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005829"(cytosol)|go:"GO:0006413"(translational initiation)|go:"GO:0008135"(translation factor activity, RNA binding)|interpro:IPR001253("Eukaryotic initiation factor 1A (eIF-1A)")|interpro:IPR006196(S1, IF1 type)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018104|rcsb pdb:1D7Q|rcsb pdb:3ZJY|rcsb pdb:4KZY|rcsb pdb:4KZZ|rcsb pdb:6YBW|rcsb pdb:6ZMW|rcsb pdb:6ZP4|rcsb pdb:7A09|reactome:R-HSA-156827|reactome:R-HSA-72649|reactome:R-HSA-72689|reactome:R-HSA-72695|reactome:R-HSA-72702|reactome:R-HSA-72706|refseq:XP_016855198.1|dip:DIP-40995N - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:Supp. figure 2 F/I|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/09 - rogid:C/YFiphF76YhqKYoM7ybw320ihw9606 - false - - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618387|imex:IM-28745-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. figure 2 F/I|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/09 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false - cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:O75822 intact:EBI-25401791 intact:EBI-366647|uniprotkb:Q9BUD2|uniprotkb:Q9H8Q2|uniprotkb:Q9UI65|ensembl:ENSP00000261868|uniprotkb:B4DUI3|uniprotkb:F5H425 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:eif3j_human(display_long)|uniprotkb:EIF3J(gene name)|psi-mi:EIF3J(display_short)|uniprotkb:PRO0391(orf name)|uniprotkb:EIF3S1(gene name synonym)|uniprotkb:Eukaryotic translation initiation factor 3 subunit 1(gene name synonym)|uniprotkb:eIF-3-alpha(gene name synonym)|uniprotkb:eIF3 p35(gene name synonym) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618394|imex:IM-28745-27 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0586"(inhibitor) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) refseq:NP_001271264.1|refseq:NP_001271265.1|refseq:NP_003749.2|ensembl:ENSG00000104131(gene)|ensembl:ENST00000261868(transcript)|go:"GO:0001732"(formation of cytoplasmic translation initiation complex)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005829"(cytosol)|go:"GO:0005852"(eukaryotic translation initiation factor 3 complex)|go:"GO:0006413"(translational initiation)|go:"GO:0016282"(eukaryotic 43S preinitiation complex)|go:"GO:0033290"(eukaryotic 48S preinitiation complex)|go:"GO:0042802"(identical protein binding)|interpro:IPR013906(Translation initiation factor eIF3 subunit)|interpro:IPR023194|mint:O75822|rcsb pdb:2KRB|rcsb pdb:3BPJ|rcsb pdb:6YBW|rcsb pdb:6ZMW|rcsb pdb:6ZVJ|reactome:R-HSA-156827|reactome:R-HSA-72649|reactome:R-HSA-72689|reactome:R-HSA-72695|reactome:R-HSA-72702|reactome:R-HSA-72706|dip:DIP-31117N - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:Supp. figure 2 F|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/09 - rogid:dJ+uqlLn3RH30Rw3COnJ+/nkk+I9606 - false - - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618394|imex:IM-28745-27 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. figure 2 F|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/09 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false - cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 intact:EBI-26452941 intact:EBI-25401791 intact:EBI-25401791 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618475|imex:IM-28745-43 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:1302"(stable complex) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:Supp. figure 2 I|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/09 - - - false cy3 label:n-n - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P47813 intact:EBI-25401791 intact:EBI-1045377|uniprotkb:Q0VGC2|uniprotkb:Q5JPS5|uniprotkb:Q5JPS6|uniprotkb:B2R5U5|ensembl:ENSP00000368927 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:if1ax_human(display_long)|uniprotkb:Eukaryotic translation initiation factor 4C(gene name synonym)|uniprotkb:EIF1AX(gene name)|psi-mi:EIF1AX(display_short)|uniprotkb:EIF1A(gene name synonym)|uniprotkb:EIF4C(gene name synonym) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618475|imex:IM-28745-43 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) refseq:NP_001403.1|ensembl:ENSG00000173674(gene)|ensembl:ENST00000379607(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005829"(cytosol)|go:"GO:0006413"(translational initiation)|go:"GO:0008135"(translation factor activity, RNA binding)|interpro:IPR001253("Eukaryotic initiation factor 1A (eIF-1A)")|interpro:IPR006196(S1, IF1 type)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018104|rcsb pdb:1D7Q|rcsb pdb:3ZJY|rcsb pdb:4KZY|rcsb pdb:4KZZ|rcsb pdb:6YBW|rcsb pdb:6ZMW|rcsb pdb:6ZP4|rcsb pdb:7A09|reactome:R-HSA-156827|reactome:R-HSA-72649|reactome:R-HSA-72689|reactome:R-HSA-72695|reactome:R-HSA-72702|reactome:R-HSA-72706|refseq:XP_016855198.1|dip:DIP-40995N - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:Supp. figure 2 I|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/09 - rogid:C/YFiphF76YhqKYoM7ybw320ihw9606 - false cy3 label:n-n - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618475|imex:IM-28745-43 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. figure 2 I|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/09 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false cy3 label:n-n cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26619737|imex:IM-28745-49 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. figure 3D|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/11 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false - cy3 label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P41567 intact:EBI-25401791 intact:EBI-726200|intact:EBI-1059306|uniprotkb:Q9UNQ9|ensembl:ENSP00000419449 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:eif1_human(display_long)|uniprotkb:Protein translation factor SUI1 homolog(gene name synonym)|uniprotkb:Sui1iso1(gene name synonym)|uniprotkb:A121(gene name synonym)|uniprotkb:EIF1(gene name)|psi-mi:EIF1(display_short)|uniprotkb:SUI1(gene name synonym) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619743|imex:IM-28745-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0840"(stimulator) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) refseq:NP_005792.1|ensembl:ENSG00000173812(gene)|ensembl:ENST00000469257(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006446"(regulation of translational initiation)|go:"GO:0008135"(translation factor activity, RNA binding)|go:"GO:0016282"(eukaryotic 43S preinitiation complex)|go:"GO:0043024"(ribosomal small subunit binding)|interpro:IPR001950(Translation initiation factor SUI1)|interpro:IPR005874(Eukaryotic translation initiation factor SUI1)|interpro:IPR036877|rcsb pdb:2IF1|rcsb pdb:4KZX|rcsb pdb:4KZY|rcsb pdb:6YBW|rcsb pdb:6ZMW|rcsb pdb:6ZP4|rcsb pdb:6ZVJ|rcsb pdb:7A09|dip:DIP-50783N - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:Supp. figure 3D|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/11 - rogid:NVdVREA4Yjtxp30eKQr0GUvlYhg9606 - false - - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619743|imex:IM-28745-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. figure 3D|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/11 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false - cy3 label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619750|imex:IM-28745-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0941"(competitor) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. figure 3D|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/11 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false - - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619750|imex:IM-28745-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. figure 3D|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/11 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false - cy3 label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P41567 intact:EBI-25401791 intact:EBI-726200|intact:EBI-1059306|uniprotkb:Q9UNQ9|ensembl:ENSP00000419449 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:eif1_human(display_long)|uniprotkb:Protein translation factor SUI1 homolog(gene name synonym)|uniprotkb:Sui1iso1(gene name synonym)|uniprotkb:A121(gene name synonym)|uniprotkb:EIF1(gene name)|psi-mi:EIF1(display_short)|uniprotkb:SUI1(gene name synonym) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618773|imex:IM-28745-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0840"(stimulator) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) refseq:NP_005792.1|ensembl:ENSG00000173812(gene)|ensembl:ENST00000469257(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006446"(regulation of translational initiation)|go:"GO:0008135"(translation factor activity, RNA binding)|go:"GO:0016282"(eukaryotic 43S preinitiation complex)|go:"GO:0043024"(ribosomal small subunit binding)|interpro:IPR001950(Translation initiation factor SUI1)|interpro:IPR005874(Eukaryotic translation initiation factor SUI1)|interpro:IPR036877|rcsb pdb:2IF1|rcsb pdb:4KZX|rcsb pdb:4KZY|rcsb pdb:6YBW|rcsb pdb:6ZMW|rcsb pdb:6ZP4|rcsb pdb:6ZVJ|rcsb pdb:7A09|dip:DIP-50783N - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:2 B-F, Supp. Figure 3G-K,4C|kinetics:"The estimated equilibrium dissociation constant (KD) of the NSP1 interaction with eIF1–40S subunit complexes was ≤∼10 nM."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kon:0.3x10^-12(per mole per second) 2021/01/25 2021/02/10 - rogid:NVdVREA4Yjtxp30eKQr0GUvlYhg9606 - false biotin tag:c-c - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618773|imex:IM-28745-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:2 B-F, Supp. Figure 3G-K,4C|kinetics:"The estimated equilibrium dissociation constant (KD) of the NSP1 interaction with eIF1–40S subunit complexes was ≤∼10 nM."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kon:0.3x10^-12(per mole per second) 2021/01/25 2021/02/10 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:c-c cy3 label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:O75822 intact:EBI-25401791 intact:EBI-366647|uniprotkb:Q9BUD2|uniprotkb:Q9H8Q2|uniprotkb:Q9UI65|ensembl:ENSP00000261868|uniprotkb:B4DUI3|uniprotkb:F5H425 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:eif3j_human(display_long)|uniprotkb:EIF3J(gene name)|psi-mi:EIF3J(display_short)|uniprotkb:PRO0391(orf name)|uniprotkb:EIF3S1(gene name synonym)|uniprotkb:Eukaryotic translation initiation factor 3 subunit 1(gene name synonym)|uniprotkb:eIF-3-alpha(gene name synonym)|uniprotkb:eIF3 p35(gene name synonym) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618888|imex:IM-28745-45 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0586"(inhibitor) psi-mi:"MI:0496"(bait) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) refseq:NP_001271264.1|refseq:NP_001271265.1|refseq:NP_003749.2|ensembl:ENSG00000104131(gene)|ensembl:ENST00000261868(transcript)|go:"GO:0001732"(formation of cytoplasmic translation initiation complex)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005829"(cytosol)|go:"GO:0005852"(eukaryotic translation initiation factor 3 complex)|go:"GO:0006413"(translational initiation)|go:"GO:0016282"(eukaryotic 43S preinitiation complex)|go:"GO:0033290"(eukaryotic 48S preinitiation complex)|go:"GO:0042802"(identical protein binding)|interpro:IPR013906(Translation initiation factor eIF3 subunit)|interpro:IPR023194|mint:O75822|rcsb pdb:2KRB|rcsb pdb:3BPJ|rcsb pdb:6YBW|rcsb pdb:6ZMW|rcsb pdb:6ZVJ|reactome:R-HSA-156827|reactome:R-HSA-72649|reactome:R-HSA-72689|reactome:R-HSA-72695|reactome:R-HSA-72702|reactome:R-HSA-72706|dip:DIP-31117N - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:2 C|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/10 - rogid:dJ+uqlLn3RH30Rw3COnJ+/nkk+I9606 - false biotin tag:c-c - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P41567 intact:EBI-25401791 intact:EBI-726200|intact:EBI-1059306|uniprotkb:Q9UNQ9|ensembl:ENSP00000419449 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:eif1_human(display_long)|uniprotkb:Protein translation factor SUI1 homolog(gene name synonym)|uniprotkb:Sui1iso1(gene name synonym)|uniprotkb:A121(gene name synonym)|uniprotkb:EIF1(gene name)|psi-mi:EIF1(display_short)|uniprotkb:SUI1(gene name synonym) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618888|imex:IM-28745-45 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) refseq:NP_005792.1|ensembl:ENSG00000173812(gene)|ensembl:ENST00000469257(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006446"(regulation of translational initiation)|go:"GO:0008135"(translation factor activity, RNA binding)|go:"GO:0016282"(eukaryotic 43S preinitiation complex)|go:"GO:0043024"(ribosomal small subunit binding)|interpro:IPR001950(Translation initiation factor SUI1)|interpro:IPR005874(Eukaryotic translation initiation factor SUI1)|interpro:IPR036877|rcsb pdb:2IF1|rcsb pdb:4KZX|rcsb pdb:4KZY|rcsb pdb:6YBW|rcsb pdb:6ZMW|rcsb pdb:6ZP4|rcsb pdb:6ZVJ|rcsb pdb:7A09|dip:DIP-50783N - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:2 C|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/10 - rogid:NVdVREA4Yjtxp30eKQr0GUvlYhg9606 - false biotin tag:c-c - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26618888|imex:IM-28745-45 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:2 C|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/10 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:c-c cy3 label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-26452941 intact:EBI-25475847 intact:EBI-25401791 psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619246|imex:IM-28745-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:1302"(stable complex) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - chain-seq-start:1|chain-seq-end:180 curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:2 E/F, 4D, Supp. figure 3K, 4C|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/11 rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - - false cy3 label:n-n biotin tag:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P47813 intact:EBI-25401791 intact:EBI-1045377|uniprotkb:Q0VGC2|uniprotkb:Q5JPS5|uniprotkb:Q5JPS6|uniprotkb:B2R5U5|ensembl:ENSP00000368927 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:if1ax_human(display_long)|uniprotkb:Eukaryotic translation initiation factor 4C(gene name synonym)|uniprotkb:EIF1AX(gene name)|psi-mi:EIF1AX(display_short)|uniprotkb:EIF1A(gene name synonym)|uniprotkb:EIF4C(gene name synonym) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619258|imex:IM-28745-5 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) refseq:NP_001403.1|ensembl:ENSG00000173674(gene)|ensembl:ENST00000379607(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005829"(cytosol)|go:"GO:0006413"(translational initiation)|go:"GO:0008135"(translation factor activity, RNA binding)|interpro:IPR001253("Eukaryotic initiation factor 1A (eIF-1A)")|interpro:IPR006196(S1, IF1 type)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018104|rcsb pdb:1D7Q|rcsb pdb:3ZJY|rcsb pdb:4KZY|rcsb pdb:4KZZ|rcsb pdb:6YBW|rcsb pdb:6ZMW|rcsb pdb:6ZP4|rcsb pdb:7A09|reactome:R-HSA-156827|reactome:R-HSA-72649|reactome:R-HSA-72689|reactome:R-HSA-72695|reactome:R-HSA-72702|reactome:R-HSA-72706|refseq:XP_016855198.1|dip:DIP-40995N - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:2 E-F, Supp. figure 3K|comment:"The 43S preinitiation complex, combined eIF1 and eIF1A and 40S, combined eIF1, eIF1A plus eIF3 minus the \"j\" subunit and 40S, also bound NSP1."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/10 - rogid:C/YFiphF76YhqKYoM7ybw320ihw9606 - false biotin tag:c-c - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619258|imex:IM-28745-5 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:2 E-F, Supp. figure 3K|comment:"The 43S preinitiation complex, combined eIF1 and eIF1A and 40S, combined eIF1, eIF1A plus eIF3 minus the \"j\" subunit and 40S, also bound NSP1."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/10 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:c-c cy3 label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26619444 uniprotkb:P0DTD1-PRO_0000449619 - intact:EBI-25475847 psi-mi:wt_hcv ires +0(display_short)|psi-mi:EBI-26619444(display_long) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619436|imex:IM-28745-61 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_038882.1(see-also) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon)." chain-seq-start:1|chain-seq-end:180 figure legend:3, 4, Supp. figure 4C/I/J,5B|kinetics:There was an estimated KD of ≤∼1 nM.|comment:"Inclusion of eIFs used for initiation by the HCV IRES (eIF1, eIF1A, eIF5, eIF3deltaj, and TC-GMPPNP) on HCV+12 further slowed, by eightfold, the NSP1 apparent association rate."|comment:"Kds of the NSP1 interaction with the IRES–40S subunit complex was increased at least 2,000-fold (KD ≥ 2 uM to 3 uM) by long segments of RNA downstream of the start codon."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/12 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:1-1|mutation with no effect:402-402|mutation with no effect:402-402|mutation decreasing interaction strength:402-402|mutation decreasing interaction strength:402-402 cy3 label:n-n|mutation decreasing interaction strength:164-165 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26619444 intact:EBI-26452941 - intact:EBI-25401791 psi-mi:wt_hcv ires +0(display_short)|psi-mi:EBI-26619444(display_long) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619436|imex:IM-28745-61 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:1302"(stable complex) refseq:NC_038882.1(see-also) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon)." curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:3, 4, Supp. figure 4C/I/J,5B|kinetics:There was an estimated KD of ≤∼1 nM.|comment:"Inclusion of eIFs used for initiation by the HCV IRES (eIF1, eIF1A, eIF5, eIF3deltaj, and TC-GMPPNP) on HCV+12 further slowed, by eightfold, the NSP1 apparent association rate."|comment:"Kds of the NSP1 interaction with the IRES–40S subunit complex was increased at least 2,000-fold (KD ≥ 2 uM to 3 uM) by long segments of RNA downstream of the start codon."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/12 - - - false biotin tag:1-1|mutation with no effect:402-402|mutation with no effect:402-402|mutation decreasing interaction strength:402-402|mutation decreasing interaction strength:402-402 cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26619464 uniprotkb:P0DTD1-PRO_0000449619 - intact:EBI-25475847 psi-mi:"hcv ires(delta-dii)+0"(display_short)|psi-mi:EBI-26619464(display_long) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619452|imex:IM-28745-47 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_038882.1(see-also) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon) but with domain 2 replaced." chain-seq-start:1|chain-seq-end:180 figure legend:"3 D-G, (4), Supp. figure 4D/E"|comment:"There was a striking delay in NSP1 association with the 40S–HCV(Delta dII)+0 complex with multiphasic behavior."|comment:"Figure 4 shows results for a HCV IRES(Delta dII) +48 construct;however no comparison is given in the same figure with regard to the +0 construct, so it is not possible to state it the +48 construct showed reduced binding in this instance."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/11 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:1-1|mutation decreasing interaction strength:339-339 cy3 label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26619464 intact:EBI-26452941 - intact:EBI-25401791 psi-mi:"hcv ires(delta-dii)+0"(display_short)|psi-mi:EBI-26619464(display_long) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619452|imex:IM-28745-47 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:1302"(stable complex) refseq:NC_038882.1(see-also) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon) but with domain 2 replaced." curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:"3 D-G, (4), Supp. figure 4D/E"|comment:"There was a striking delay in NSP1 association with the 40S–HCV(Delta dII)+0 complex with multiphasic behavior."|comment:"Figure 4 shows results for a HCV IRES(Delta dII) +48 construct;however no comparison is given in the same figure with regard to the +0 construct, so it is not possible to state it the +48 construct showed reduced binding in this instance."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/11 - - - false biotin tag:1-1|mutation decreasing interaction strength:339-339 cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26619444 uniprotkb:P0C6X7-PRO_0000037309 - intact:EBI-25475797 psi-mi:wt_hcv ires +0(display_short)|psi-mi:EBI-26619444(display_long) psi-mi:p0c6x7-pro_0000037309(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:694009(sars-cov)|taxid:694009(Human SARS coronavirus) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619788|imex:IM-28745-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_038882.1(see-also) intact:EBI-7843867(chain-parent)|uniprotkb:P0C6X7(chain-parent) - comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon)." chain-seq-start:1|chain-seq-end:180 figure legend:3, Supp. figure 4G|comment:SARS Nsp1|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kd:2.0x10^-9(molar) 2021/01/25 2021/02/11 - rogid:qbwEpGmE3J035KnL6QbEZ/HRJAw694009 - false biotin tag:1-1 cy3 label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26619444 intact:EBI-26452941 - intact:EBI-25401791 psi-mi:wt_hcv ires +0(display_short)|psi-mi:EBI-26619444(display_long) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619788|imex:IM-28745-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:1302"(stable complex) refseq:NC_038882.1(see-also) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon)." curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:3, Supp. figure 4G|comment:SARS Nsp1|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kd:2.0x10^-9(molar) 2021/01/25 2021/02/11 - - - false biotin tag:1-1 cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26619444 uniprotkb:A0A088DIE1 - intact:EBI-26619893 psi-mi:wt_hcv ires +0(display_short)|psi-mi:EBI-26619444(display_long) psi-mi:a0a088die1_9betc(display_long)|uniprotkb:Helicase(gene name synonym)|uniprotkb:Host translation inhibitor nsp1(gene name synonym)|uniprotkb:Leader protein(gene name synonym)|uniprotkb:NendoU(gene name synonym)|uniprotkb:Non-structural protein 10(gene name synonym)|uniprotkb:Non-structural protein 2(gene name synonym)|uniprotkb:Non-structural protein 3(gene name synonym)|uniprotkb:Non-structural protein 4(gene name synonym)|uniprotkb:Non-structural protein 6(gene name synonym)|uniprotkb:Non-structural protein 7(gene name synonym)|uniprotkb:Non-structural protein 8(gene name synonym)|uniprotkb:Non-structural protein 9(gene name synonym)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:Papain-like proteinase(gene name synonym)|uniprotkb:RNA-directed RNA polymerase(gene name synonym)|uniprotkb:Replicase polyprotein 1ab(gene name synonym)|uniprotkb:Uridylate-specific endoribonuclease(gene name synonym)|uniprotkb:p65 homolog(gene name synonym)|uniprotkb:3C-like proteinase(gene name synonym)|uniprotkb:Growth factor-like peptide(gene name synonym)|uniprotkb:Guanine-N7 methyltransferase(gene name synonym)|uniprotkb:nsp12(gene name synonym)|uniprotkb:nsp13(gene name synonym)|uniprotkb:nsp14(gene name synonym)|uniprotkb:nsp15(gene name synonym)|uniprotkb:nsp16(gene name synonym)|uniprotkb:nsp5(gene name synonym) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:1541205(Bat Hp-betacoronavirus/Zhejiang2013)|taxid:1541205(Bat Hp-betacoronavirus/Zhejiang2013) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619879|imex:IM-28745-29 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_038882.1(see-also) go:"GO:0002151"(G-quadruplex RNA binding)|go:"GO:0003678"(DNA helicase activity)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0003727"(single-stranded RNA binding)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0004532"(exoribonuclease activity)|go:"GO:0004843"(thiol-dependent deubiquitinase)|go:"GO:0005524"(ATP binding)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006351"(transcription, DNA-templated)|go:"GO:0008168"(methyltransferase activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019082"(viral protein processing)|go:"GO:0032259"(methylation)|go:"GO:0033644"(host cell membrane)|go:"GO:0039502"(suppression by virus of host type I interferon-mediated signaling pathway)|go:"GO:0039520"(induction by virus of host autophagy)|go:"GO:0039579"(suppression by virus of host ISG15-protein conjugation)|go:"GO:0039595"(induction by virus of catabolism of host mRNA)|go:"GO:0039644"(suppression by virus of host NF-kappaB cascade)|go:"GO:0039648"(modulation by virus of host protein ubiquitination)|go:"GO:0039694"(viral RNA genome replication)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:0004519"(endonuclease activity)|interpro:IPR022733|interpro:IPR044315|interpro:IPR044322|interpro:IPR044330|interpro:IPR044343|interpro:IPR044353|interpro:IPR044357|interpro:IPR044367|interpro:IPR044371|interpro:IPR044389|interpro:IPR044401|refseq:YP_009072438.1|interpro:IPR027351|interpro:IPR043609|interpro:IPR043608|interpro:IPR043606|interpro:IPR043613|interpro:IPR043615|interpro:IPR043611|interpro:IPR043612|interpro:IPR043610|interpro:IPR027352|interpro:IPR043502|interpro:IPR041679|interpro:IPR037227|interpro:IPR002589(Appr-1-p processing)|interpro:IPR043472|interpro:IPR042570|interpro:IPR036333|interpro:IPR009466(NSP11)|interpro:IPR043174|interpro:IPR042515|interpro:IPR009461(Coronavirus NSP13)|interpro:IPR021590|interpro:IPR038030|interpro:IPR024375|interpro:IPR032592|interpro:IPR038400|interpro:IPR038083|interpro:IPR032505|interpro:IPR038123|interpro:IPR014828(Nsp7 replicase)|interpro:IPR037204|interpro:IPR014829(Nsp8 replicase)|interpro:IPR037230|interpro:IPR014822(Nsp9 replicase)|interpro:IPR036499|interpro:IPR027417|interpro:IPR013016(Peptidase C30/C16)|interpro:IPR008740(Peptidase C30, Coronavirus endopeptidase)|interpro:IPR043477|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR043504|interpro:IPR043177|interpro:IPR043503|interpro:IPR043178|interpro:IPR001205(RNA-directed RNA polymerase, P3D protein)|interpro:IPR007094(RNA-directed RNA polymerase, positive-strand RNA eukaryotic virus)|interpro:IPR009469(RNA polymerase, N-terminal, coronaviral)|interpro:IPR018995|interpro:IPR029063 - comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon)." - figure legend:3 D-G, Supp. figure 4H|comment:Bat-Hp Nsp1|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kd:1.0x10^-9(molar) 2021/01/25 2021/02/11 - rogid:F8VmcDKvyrM34UezTRXBEJjWWqI1541205 - false biotin tag:1-1 cy3 label:n-n|sufficient binding region:13..13-126..126 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26619444 intact:EBI-26452941 - intact:EBI-25401791 psi-mi:wt_hcv ires +0(display_short)|psi-mi:EBI-26619444(display_long) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26619879|imex:IM-28745-29 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:1302"(stable complex) refseq:NC_038882.1(see-also) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon)." curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:3 D-G, Supp. figure 4H|comment:Bat-Hp Nsp1|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kd:1.0x10^-9(molar) 2021/01/25 2021/02/11 - - - false biotin tag:1-1 cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26620078 uniprotkb:P0DTD1-PRO_0000449619 - intact:EBI-25475847 psi-mi:"model mrna (m+41)"(display_short)|psi-mi:EBI-26620078(display_long) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26620059|imex:IM-28745-13 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - - chain-seq-start:1|chain-seq-end:180 figure legend:4 B-D, Supp. figure 5 C/D|comment:Pre-formed M+41–40S-Cy5 complexes were tethered to the ZMW imaging surface in the presence of eIF1, eIF1A, eIF3, eIF5, and TC-GMPPNP.|comment:Inefficient association of NSP1 with the mRNA–40S subunit complexes was observed.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/11 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:63-63 cy3 label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26620078 intact:EBI-26452941 - intact:EBI-25401791 psi-mi:"model mrna (m+41)"(display_short)|psi-mi:EBI-26620078(display_long) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26620059|imex:IM-28745-13 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:4 B-D, Supp. figure 5 C/D|comment:Pre-formed M+41–40S-Cy5 complexes were tethered to the ZMW imaging surface in the presence of eIF1, eIF1A, eIF3, eIF5, and TC-GMPPNP.|comment:Inefficient association of NSP1 with the mRNA–40S subunit complexes was observed.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/11 - - - false biotin tag:63-63 cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26651004|imex:IM-28745-31 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:4 B-D, Supp. figure 5 E-H|comment:Pre-formed Cy5-M+41–40S subunit complexes were tethered to the ZMW imaging surface in the presence of eIF1, eIF1A, eIF3, eIF5, and TC-GMPPNP.|comment:Inefficient association of NSP1 with the mRNA–40S subunit complexes was observed.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/12 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:c-c cy3 label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 intact:EBI-26620078 intact:EBI-25401791 - psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"model mrna (m+41)"(display_short)|psi-mi:EBI-26620078(display_long) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:32630(synthetic construct)|taxid:32630(synthetic construct) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26651004|imex:IM-28745-31 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0320"(ribonucleic acid) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:4 B-D, Supp. figure 5 E-H|comment:Pre-formed Cy5-M+41–40S subunit complexes were tethered to the ZMW imaging surface in the presence of eIF1, eIF1A, eIF3, eIF5, and TC-GMPPNP.|comment:Inefficient association of NSP1 with the mRNA–40S subunit complexes was observed.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/12 - - - false biotin tag:c-c cy5 label:63-63 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26651326 uniprotkb:P0DTD1-PRO_0000449619 - intact:EBI-25475847 psi-mi:crpv ires+1(display_short)|psi-mi:EBI-26651326(display_long) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26652433|imex:IM-28745-53 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:0326"(protein) - intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - - chain-seq-start:1|chain-seq-end:180 figure legend:5 E/F, Supp.figure 7D|comment:"Inclusion of 60S subunits to yield 80S–CrPV+1 complexes inhibited NSP1 association, (an NSP1 binding event was seen in ∼30% of traces). This is similar to the inhibition observed on both 80S-CrPV+6 and 80S–CrPV+48 complexes."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/15 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:1-1|mutation decreasing interaction strength:191-191|mutation decreasing interaction strength:191-191|sufficient binding region:?-?|sufficient binding region:?-? cyanine label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26651326 intact:EBI-26452941 - intact:EBI-25401791 psi-mi:crpv ires+1(display_short)|psi-mi:EBI-26651326(display_long) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26652433|imex:IM-28745-53 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:1302"(stable complex) - evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:5 E/F, Supp.figure 7D|comment:"Inclusion of 60S subunits to yield 80S–CrPV+1 complexes inhibited NSP1 association, (an NSP1 binding event was seen in ∼30% of traces). This is similar to the inhibition observed on both 80S-CrPV+6 and 80S–CrPV+48 complexes."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/15 - - - false biotin tag:1-1|mutation decreasing interaction strength:191-191|mutation decreasing interaction strength:191-191|sufficient binding region:?-?|sufficient binding region:?-? cy3 label:n-n|sufficient binding region:?-?|sufficient binding region:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26651326 reactome:R-HSA-72476 - intact:EBI-25395978 psi-mi:crpv ires+1(display_short)|psi-mi:EBI-26651326(display_long) psi-mi:60s_human(display_short)|psi-mi:R-HSA-72476(display_long)|intact:"RPLP0:RPLP1:RPLP2:RPL3:RPL3L:RPL4:RPL5:RPL6:RPL7:RPL7A:RPL8:RPL9:RPL10:RPL10A:RPL10L:RPL11:RPL12:RPL13:RPL13A:RPL14:RPL15:RPL17:RPL18:RPL18A:RPL19:RPL21:RPL22:RPL22L1:RPL23:RPL23A:RPL24:RPL26:RPL26L1:RPL27:RPL27A:RPL28:RPL29:RPL30:RPL31:RPL32:RPL34:RPL35:R"(complex systematic name)|intact:60S cytosolic large ribosomal subunit(complex recommended name)|intact:LSU(complex synonym) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26652433|imex:IM-28745-53 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:1302"(stable complex) - go:"GO:0022625"(cytosolic large ribosomal subunit)|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0005737"(cytoplasm)|go:"GO:0045182"(translation regulator activity)|go:"GO:0000048"(peptidyltransferase activity)|complex portal:CPX-5183(complex-primary)|evidence ontology:"ECO:0005547"|pubmed:30075139(see-also)|pubmed:25901680(see-also) - - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Responsible for the catalytic activity of the ribosome, the peptidyltransferase activity required to catalyze peptide bond formation. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) (CPX-5223) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only six of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in the case of RPL3/RPL3L and RPL22/RPL22L1, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. A distinct lateral protuberance called the stalk is located on the large ribosomal subunit and plays a central role in the ribosome-mediated stimulation of translation factor-dependent GTP hydrolysis. The 5 acidic ribosomal P-proteins form the pentameric stalk." figure legend:5 E/F, Supp.figure 7D|comment:"Inclusion of 60S subunits to yield 80S–CrPV+1 complexes inhibited NSP1 association, (an NSP1 binding event was seen in ∼30% of traces). This is similar to the inhibition observed on both 80S-CrPV+6 and 80S–CrPV+48 complexes."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/15 - - - false biotin tag:1-1|mutation decreasing interaction strength:191-191|mutation decreasing interaction strength:191-191|sufficient binding region:?-?|sufficient binding region:?-? cy5 label:c-c|sufficient binding region:?-?|sufficient binding region:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26651326 uniprotkb:P0DTD1-PRO_0000449619 - intact:EBI-25475847 psi-mi:crpv ires+1(display_short)|psi-mi:EBI-26651326(display_long) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26655166|imex:IM-28745-55 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:0326"(protein) - intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - - chain-seq-start:1|chain-seq-end:180 figure legend:5 E/F, Supp. figure 7 C/D|comment:When NSP1 was added to 40S–CrPV+1 complexes, slower and less efficient Cy5.5-NSP1 association relative to that of 40S–HCV+0 complexes was observed.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/15 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:1-1|sufficient binding region:?-? cyanine label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26651326 intact:EBI-26452941 - intact:EBI-25401791 psi-mi:crpv ires+1(display_short)|psi-mi:EBI-26651326(display_long) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26655166|imex:IM-28745-55 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:1302"(stable complex) - evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:5 E/F, Supp. figure 7 C/D|comment:When NSP1 was added to 40S–CrPV+1 complexes, slower and less efficient Cy5.5-NSP1 association relative to that of 40S–HCV+0 complexes was observed.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/15 - - - false biotin tag:1-1|sufficient binding region:?-? cy3 label:n-n|sufficient binding region:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26619444 uniprotkb:P0DTD1-PRO_0000449619 - intact:EBI-25475847 psi-mi:wt_hcv ires +0(display_short)|psi-mi:EBI-26619444(display_long) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26655414|imex:IM-28745-35 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_038882.1(see-also) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon)." chain-seq-start:1|chain-seq-end:180 figure legend:5 F, Supp. figure 7D|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/15 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:1-1|sufficient binding region:?-? cyanine label:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26619444 intact:EBI-26452941 - intact:EBI-25401791 psi-mi:wt_hcv ires +0(display_short)|psi-mi:EBI-26619444(display_long) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26655414|imex:IM-28745-35 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:1302"(stable complex) refseq:NC_038882.1(see-also) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon)." curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:5 F, Supp. figure 7D|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/15 - - - false biotin tag:1-1|sufficient binding region:?-? cy3 label:n-n|sufficient binding region:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26619444 uniprotkb:P0DTD1-PRO_0000449619 - intact:EBI-25475847 psi-mi:wt_hcv ires +0(display_short)|psi-mi:EBI-26619444(display_long) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26655867|imex:IM-28745-37 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:0326"(protein) refseq:NC_038882.1(see-also) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon)." chain-seq-start:1|chain-seq-end:180 figure legend:6 A/B/C/D, Supp. figure 8|comment:"Preformed NSP1–40S complexes were added to ZMWs with surface-immobilized model mRNAs. NSP1 was long lived on the ribosomal subunit, for both HCV and CrPV +0/1 and +48 RNAs. Following initial loss of NSP1 signal, NSP1 readily re-associated with the same 40S–HCV+0 complex but not the 40S–HCV+48 complex. (figure 6D and Supp. figure 8)"|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/15 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:1-1|mutation with no effect:402-402 cyanine label:n-n|sufficient binding region:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26619444 intact:EBI-26452941 - intact:EBI-25401791 psi-mi:wt_hcv ires +0(display_short)|psi-mi:EBI-26619444(display_long) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26655867|imex:IM-28745-37 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:1302"(stable complex) refseq:NC_038882.1(see-also) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon)." curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:6 A/B/C/D, Supp. figure 8|comment:"Preformed NSP1–40S complexes were added to ZMWs with surface-immobilized model mRNAs. NSP1 was long lived on the ribosomal subunit, for both HCV and CrPV +0/1 and +48 RNAs. Following initial loss of NSP1 signal, NSP1 readily re-associated with the same 40S–HCV+0 complex but not the 40S–HCV+48 complex. (figure 6D and Supp. figure 8)"|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/15 - - - false biotin tag:1-1|mutation with no effect:402-402 cy3 label:n-n|sufficient binding region:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26651326 uniprotkb:P0DTD1-PRO_0000449619 - intact:EBI-25475847 psi-mi:crpv ires+1(display_short)|psi-mi:EBI-26651326(display_long) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26656582|imex:IM-28745-17 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:0326"(protein) - intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - - chain-seq-start:1|chain-seq-end:180 figure legend:6 A/C, Supp. figure 8|comment:Preformed NSP1–40S complexes were to to ZMWs with surface-immobilized model mRNAs. Association of the NSP1–40S subunit complex with the tethered RNA had kinetics similar to 40S subunits alone. NSP1 was long lived on the ribosomal subunit. Following initial loss of NSP1 signal, NSP1 readily re-associated with the same 40S–HCV+0 complex but not the 40S–HCV+48 complex.|comment:Preformed NSP1–40S complexes were added to ZMWs with surface-immobilized model mRNAs. NSP1 was long lived on the ribosomal subunit, for both HCV and CrPV +0/1 and +48 RNAs.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/16 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:1-1|mutation with no effect:191-191 cyanine label:n-n|sufficient binding region:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26651326 intact:EBI-26452941 - intact:EBI-25401791 psi-mi:crpv ires+1(display_short)|psi-mi:EBI-26651326(display_long) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26656582|imex:IM-28745-17 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0319"(deoxyribonucleic acid) psi-mi:"MI:1302"(stable complex) - evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:6 A/C, Supp. figure 8|comment:Preformed NSP1–40S complexes were to to ZMWs with surface-immobilized model mRNAs. Association of the NSP1–40S subunit complex with the tethered RNA had kinetics similar to 40S subunits alone. NSP1 was long lived on the ribosomal subunit. Following initial loss of NSP1 signal, NSP1 readily re-associated with the same 40S–HCV+0 complex but not the 40S–HCV+48 complex.|comment:Preformed NSP1–40S complexes were added to ZMWs with surface-immobilized model mRNAs. NSP1 was long lived on the ribosomal subunit, for both HCV and CrPV +0/1 and +48 RNAs.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/16 - - - false biotin tag:1-1|mutation with no effect:191-191 cy3 label:n-n|sufficient binding region:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26620078 uniprotkb:P0DTD1-PRO_0000449619 - intact:EBI-25475847 psi-mi:"model mrna (m+41)"(display_short)|psi-mi:EBI-26620078(display_long) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26656611|imex:IM-28745-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - - chain-seq-start:1|chain-seq-end:180 figure legend:6 A/C, Supp. figure 8|comment:"Preformed NSP1–40S complexes were added to ZMWs with surface-immobilized model mRNAs. NSP1–40S subunit preinitiation complexes (in the presence of eIF1, eIF1A, eIF5, TC-GMPPNP) corecruited to M+41 model mRNAs had very short lifetimes, rapidly codeparting from the mRNA after its expected slow association."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/16 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:1-1 cyanine label:n-n|sufficient binding region:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26620078 intact:EBI-26452941 - intact:EBI-25401791 psi-mi:"model mrna (m+41)"(display_short)|psi-mi:EBI-26620078(display_long) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:32630(synthetic construct)|taxid:32630(synthetic construct) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26656611|imex:IM-28745-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:1302"(stable complex) - evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:6 A/C, Supp. figure 8|comment:"Preformed NSP1–40S complexes were added to ZMWs with surface-immobilized model mRNAs. NSP1–40S subunit preinitiation complexes (in the presence of eIF1, eIF1A, eIF5, TC-GMPPNP) corecruited to M+41 model mRNAs had very short lifetimes, rapidly codeparting from the mRNA after its expected slow association."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/16 - - - false biotin tag:1-1 cy3 label:n-n|sufficient binding region:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 intact:EBI-26619444 intact:EBI-25401791 - psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:wt_hcv ires +0(display_short)|psi-mi:EBI-26619444(display_long) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:32630(synthetic construct)|taxid:32630(synthetic construct) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26656639|imex:IM-28745-39 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0319"(deoxyribonucleic acid) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) refseq:NC_038882.1(see-also) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." comment:"A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon)." figure legend:Supp. figure 8|comment:40S complexes were added to ZMWs with surface-immobilized model mRNAs. Association of the 40S subunit complex with the tethered RNA had kinetics similar to association with the NSP1-40S complex.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/16 - - - false cy3 label:n-n biotin tag:1-1 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 intact:EBI-26651326 intact:EBI-25401791 - psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:crpv ires+1(display_short)|psi-mi:EBI-26651326(display_long) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:32630(synthetic construct)|taxid:32630(synthetic construct) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26656668|imex:IM-28745-57 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0319"(deoxyribonucleic acid) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:Supp. figure 8|comment:40S complexes were added to ZMWs with surface-immobilized model mRNAs. Association of the 40S subunit complex with the tethered RNA had kinetics similar to association with the NSP1-40S complex.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/16 - - - false cy3 label:n-n|sufficient binding region:?-? biotin tag:1-1|mutation with no effect:191-191 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 intact:EBI-26620078 intact:EBI-25401791 - psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"model mrna (m+41)"(display_short)|psi-mi:EBI-26620078(display_long) psi-mi:"MI:0013"(biophysical) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:32630(synthetic construct)|taxid:32630(synthetic construct) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26656790|imex:IM-28745-59 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0320"(ribonucleic acid) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:Supp. figure 8|comment:"Preformed 40S complexes (in the presence of eIF1, eIF1A, eIF5, TC-GMPPNP)) were added to ZMWs with surface-immobilized model mRNAs."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/16 - - - false cy3 label:n-n biotin tag:1-1 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P41567 intact:EBI-25401791 intact:EBI-726200|intact:EBI-1059306|uniprotkb:Q9UNQ9|ensembl:ENSP00000419449 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:eif1_human(display_long)|uniprotkb:Protein translation factor SUI1 homolog(gene name synonym)|uniprotkb:Sui1iso1(gene name synonym)|uniprotkb:A121(gene name synonym)|uniprotkb:EIF1(gene name)|psi-mi:EIF1(display_short)|uniprotkb:SUI1(gene name synonym) psi-mi:"MI:0976"(total internal reflection fluorescence spectroscopy) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26651026|imex:IM-28745-33 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0840"(stimulator) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) refseq:NP_005792.1|ensembl:ENSG00000173812(gene)|ensembl:ENST00000469257(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006446"(regulation of translational initiation)|go:"GO:0008135"(translation factor activity, RNA binding)|go:"GO:0016282"(eukaryotic 43S preinitiation complex)|go:"GO:0043024"(ribosomal small subunit binding)|interpro:IPR001950(Translation initiation factor SUI1)|interpro:IPR005874(Eukaryotic translation initiation factor SUI1)|interpro:IPR036877|rcsb pdb:2IF1|rcsb pdb:4KZX|rcsb pdb:4KZY|rcsb pdb:6YBW|rcsb pdb:6ZMW|rcsb pdb:6ZP4|rcsb pdb:6ZVJ|rcsb pdb:7A09|dip:DIP-50783N - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:Supp. Figure 3E/F|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kon:0.3x10^-12(per mole per second) 2021/01/25 2021/02/12 - rogid:NVdVREA4Yjtxp30eKQr0GUvlYhg9606 - false biotin tag:c-c - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0976"(total internal reflection fluorescence spectroscopy) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26651026|imex:IM-28745-33 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. Figure 3E/F|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kon:0.3x10^-12(per mole per second) 2021/01/25 2021/02/12 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:c-c cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) chebi:"CHEBI:15956" intact:EBI-26452941 intact:EBI-9690049|chebi:"CHEBI:41236"|chebi:"CHEBI:13905"|chebi:"CHEBI:22882"|chebi:"CHEBI:22884"|chebi:"CHEBI:3108" intact:EBI-25401791 psi-mi:biotin(display_short)|psi-mi:"CHEBI:15956"(display_long)|intact:"(+)-cis-Hexahydro-2-oxo-1H-thieno[3,4]imidazole-4-valeric acid"(synonym)|intact:"(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-valeric acid"(synonym)|intact:"5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid"(synonym)|intact:Biotin(synonym)|intact:BIOTIN(synonym)|intact:biotina(synonym)|intact:biotine(synonym)|intact:biotinum(synonym)|intact:"cis-(+)-Tetrahydro-2-oxothieno[3,4]imidazoline-4-valeric acid"(synonym)|intact:"cis-Hexahydro-2-oxo-1H-thieno(3,4)imidazole-4-valeric acid"(synonym)|intact:"cis-Tetrahydro-2-oxothieno(3,4-d)imidazoline-4-valeric acid"(synonym)|intact:Coenzyme R(synonym)|intact:"D-(+)-biotin"(synonym)|intact:D-Biotin(synonym)|intact:vitamin B7(synonym)|intact:Vitamin H(synonym)|intact:"5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoic acid"(iupac name) psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:"MI:0976"(total internal reflection fluorescence spectroscopy) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26651038|imex:IM-28745-15 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0941"(competitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:1302"(stable complex) - evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - inchi key:YBJHBAHKTGYVGT-ZKWXMUAHSA-N curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." figure legend:Supp. Figure 3E|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kon:0.3x10^-12(per mole per second) 2021/01/25 2021/02/12 - - - false - biotin tag:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P41567 intact:EBI-25401791 intact:EBI-726200|intact:EBI-1059306|uniprotkb:Q9UNQ9|ensembl:ENSP00000419449 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:eif1_human(display_long)|uniprotkb:Protein translation factor SUI1 homolog(gene name synonym)|uniprotkb:Sui1iso1(gene name synonym)|uniprotkb:A121(gene name synonym)|uniprotkb:EIF1(gene name)|psi-mi:EIF1(display_short)|uniprotkb:SUI1(gene name synonym) psi-mi:"MI:0976"(total internal reflection fluorescence spectroscopy) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26651038|imex:IM-28745-15 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0840"(stimulator) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) refseq:NP_005792.1|ensembl:ENSG00000173812(gene)|ensembl:ENST00000469257(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006446"(regulation of translational initiation)|go:"GO:0008135"(translation factor activity, RNA binding)|go:"GO:0016282"(eukaryotic 43S preinitiation complex)|go:"GO:0043024"(ribosomal small subunit binding)|interpro:IPR001950(Translation initiation factor SUI1)|interpro:IPR005874(Eukaryotic translation initiation factor SUI1)|interpro:IPR036877|rcsb pdb:2IF1|rcsb pdb:4KZX|rcsb pdb:4KZY|rcsb pdb:6YBW|rcsb pdb:6ZMW|rcsb pdb:6ZP4|rcsb pdb:6ZVJ|rcsb pdb:7A09|dip:DIP-50783N - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:Supp. Figure 3E|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kon:0.3x10^-12(per mole per second) 2021/01/25 2021/02/12 - rogid:NVdVREA4Yjtxp30eKQr0GUvlYhg9606 - false biotin tag:c-c - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 uniprotkb:P0DTD1-PRO_0000449619 intact:EBI-25401791 intact:EBI-25475847 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:p0dtd1-pro_0000449619(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0976"(total internal reflection fluorescence spectroscopy) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26651038|imex:IM-28745-15 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0326"(protein) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." chain-seq-start:1|chain-seq-end:180 figure legend:Supp. Figure 3E|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kon:0.3x10^-12(per mole per second) 2021/01/25 2021/02/12 - rogid:SaSNaqNfTqsYEpmhXnBT8bJZczM2697049 - false biotin tag:c-c cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 reactome:R-HSA-72476 intact:EBI-25401791 intact:EBI-25395978 psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:60s_human(display_short)|psi-mi:R-HSA-72476(display_long)|intact:"RPLP0:RPLP1:RPLP2:RPL3:RPL3L:RPL4:RPL5:RPL6:RPL7:RPL7A:RPL8:RPL9:RPL10:RPL10A:RPL10L:RPL11:RPL12:RPL13:RPL13A:RPL14:RPL15:RPL17:RPL18:RPL18A:RPL19:RPL21:RPL22:RPL22L1:RPL23:RPL23A:RPL24:RPL26:RPL26L1:RPL27:RPL27A:RPL28:RPL29:RPL30:RPL31:RPL32:RPL34:RPL35:R"(complex systematic name)|intact:60S cytosolic large ribosomal subunit(complex recommended name)|intact:LSU(complex synonym) psi-mi:"MI:0055"(fluorescent resonance energy transfer) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26651057|imex:IM-28745-51 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0583"(fluorescence donor) psi-mi:"MI:0584"(fluorescence acceptor) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:1302"(stable complex) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) go:"GO:0022625"(cytosolic large ribosomal subunit)|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0005737"(cytoplasm)|go:"GO:0045182"(translation regulator activity)|go:"GO:0000048"(peptidyltransferase activity)|complex portal:CPX-5183(complex-primary)|evidence ontology:"ECO:0005547"|pubmed:30075139(see-also)|pubmed:25901680(see-also) - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Responsible for the catalytic activity of the ribosome, the peptidyltransferase activity required to catalyze peptide bond formation. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) (CPX-5223) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only six of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in the case of RPL3/RPL3L and RPL22/RPL22L1, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. A distinct lateral protuberance called the stalk is located on the large ribosomal subunit and plays a central role in the ribosome-mediated stimulation of translation factor-dependent GTP hydrolysis. The 5 acidic ribosomal P-proteins form the pentameric stalk." figure legend:5 B/C/E|comment:The number of nucleotides downstream of the CCU codon is not stated for this assay, but has assumed to be +1.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/15 - - - false cy3 label:n-n cy5 label:c-c - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) intact:EBI-26452941 intact:EBI-26651326 intact:EBI-25401791 - psi-mi:40s_human-1(display_short)|psi-mi:EBI-26452941(display_long)|intact:SSU(complex synonym)|intact:40S cytosolic small ribosomal subunit(complex recommended name)|intact:"FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29"(complex systematic name) psi-mi:crpv ires+1(display_short)|psi-mi:EBI-26651326(display_long) psi-mi:"MI:0055"(fluorescent resonance energy transfer) Lapointe et al. (2021) imex:IM-28745|pubmed:33479166 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:32630(synthetic construct)|taxid:32630(synthetic construct) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-26651057|imex:IM-28745-51 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0583"(fluorescence donor) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:1302"(stable complex) psi-mi:"MI:0319"(deoxyribonucleic acid) evidence ontology:"ECO:0005547"|go:"GO:0002181"(cytoplasmic translation)|go:"GO:0022627"(cytosolic small ribosomal subunit)|go:"GO:0045182"(translation regulator activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0000049"(tRNA binding)|pubmed:30075139(see-also)|pubmed:25901680(see-also)|complex portal:CPX-5223(complex-primary) - - curated-complex:"Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA."|complex-properties:"The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs." - figure legend:5 B/C/E|comment:The number of nucleotides downstream of the CCU codon is not stated for this assay, but has assumed to be +1.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2021/01/25 2021/02/15 - - - false cy3 label:n-n biotin tag:1-1 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence)