#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q96E22	uniprotkb:Q86SQ9	intact:EBI-6949352|ensembl:ENSP00000357480|uniprotkb:O00251|intact:MINT-3052208|uniprotkb:B2RWQ4	intact:EBI-26942900|uniprotkb:B7Z4B9|uniprotkb:B7ZB20|uniprotkb:D3DPK7|uniprotkb:D3DPK8|uniprotkb:D3DPK9|uniprotkb:E9KL43|uniprotkb:Q5T0A4|uniprotkb:Q8NE90|uniprotkb:Q9BTG5|uniprotkb:Q9BTK3|uniprotkb:Q9H905|ensembl:ENSP00000236342	psi-mi:ngbr_human(display_long)|uniprotkb:NUS1(gene name)|psi-mi:NUS1(display_short)|uniprotkb:C6orf68(gene name synonym)|uniprotkb:NGBR(gene name synonym)|uniprotkb:Nuclear undecaprenyl pyrophosphate synthase 1 homolog(gene name synonym)|uniprotkb:Nogo-B receptor(gene name synonym)|uniprotkb:Cis-prenyltransferase subunit NgBR(gene name synonym)	psi-mi:dhdds_human(display_long)|uniprotkb:DHDDS(gene name)|psi-mi:DHDDS(display_short)|uniprotkb:Cis-isoprenyltransferase(gene name synonym)|uniprotkb:Cis-prenyltransferase subunit hCIT(gene name synonym)|uniprotkb:Epididymis tissue protein Li 189m(gene name synonym)|uniprotkb:HDS(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Edani et al. (2020)	pubmed:32817466|imex:IM-28941	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-26971622|imex:IM-28941-1|wwpdb:6w2l	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000153989(gene)|ensembl:ENST00000368494(transcript)|go:"GO:0001525"(angiogenesis)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006489"(dolichyl diphosphate biosynthetic process)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019408"(dolichol biosynthetic process)|rcsb pdb:6W2L|rcsb pdb:6Z1N|reactome:R-HSA-446199|reactome:R-HSA-4755609|go:"GO:0030154"(cell differentiation)|go:"GO:0032383"(regulation of intracellular cholesterol transport)|go:"GO:0035268"(protein mannosylation)|go:"GO:0038084"(vascular endothelial growth factor signaling pathway)|go:"GO:0042632"(cholesterol homeostasis)|go:"GO:0043536"(positive regulation of blood vessel endothelial cell migration)|go:"GO:0045547"(dehydrodolichyl diphosphate synthase activity)|go:"GO:0046872"(metal ion binding)|go:"GO:0051000"(positive regulation of nitric-oxide synthase activity)|go:"GO:1904423"(dehydrodolichyl diphosphate synthase complex)|interpro:IPR001441(Di-trans-poly-cis-decaprenylcistransferase)|interpro:IPR036424|interpro:IPR038887|refseq:NP_612468.1|dip:DIP-61225N	refseq:XP_016857869.1|refseq:NP_001230493.1|refseq:NP_001230494.1|refseq:NP_079163.2|refseq:NP_995583.1|refseq:XP_006710975.1|refseq:XP_006710976.1|refseq:XP_006710977.1|refseq:XP_011540485.1|refseq:XP_011540486.1|refseq:XP_011540488.1|refseq:XP_016857868.1|ensembl:ENSG00000117682(gene)|ensembl:ENST00000236342(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0006489"(dolichyl diphosphate biosynthetic process)|go:"GO:0016094"(polyprenol biosynthetic process)|go:"GO:0045547"(dehydrodolichyl diphosphate synthase activity)|go:"GO:0046872"(metal ion binding)|go:"GO:1904423"(dehydrodolichyl diphosphate synthase complex)|interpro:IPR001441(Di-trans-poly-cis-decaprenylcistransferase)|interpro:IPR018520|interpro:IPR036424|rcsb pdb:6W2L|rcsb pdb:6Z1N|reactome:R-HSA-446199|reactome:R-HSA-4755609	-	comment:mint|function:Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator. Able to regulate vascular remodeling and angiogenesis. Its similarity with UPP synthetase proteins suggests that it may act as a scaffold for the binding of isoprenyl lipids and/or prenylated proteins|comment:homomint	-	figure legend:Supp.Table 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2021/05/07	2021/05/07	rogid:htBbXzyI2bJDFvyuFFWIeAnF3sc9606	rogid:Ernk6WWm+mdwwms7pvGchWbmPfE9606	rigid:MMv8aSQKG+PL02D9HCGYXAQAAkM	false	sufficient binding region:80-293	sufficient binding region:2-330	1	1	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q96E22	uniprotkb:Q86SQ9	intact:EBI-6949352|ensembl:ENSP00000357480|uniprotkb:O00251|intact:MINT-3052208|uniprotkb:B2RWQ4	intact:EBI-26942900|uniprotkb:B7Z4B9|uniprotkb:B7ZB20|uniprotkb:D3DPK7|uniprotkb:D3DPK8|uniprotkb:D3DPK9|uniprotkb:E9KL43|uniprotkb:Q5T0A4|uniprotkb:Q8NE90|uniprotkb:Q9BTG5|uniprotkb:Q9BTK3|uniprotkb:Q9H905|ensembl:ENSP00000236342	psi-mi:ngbr_human(display_long)|uniprotkb:NUS1(gene name)|psi-mi:NUS1(display_short)|uniprotkb:C6orf68(gene name synonym)|uniprotkb:NGBR(gene name synonym)|uniprotkb:Nuclear undecaprenyl pyrophosphate synthase 1 homolog(gene name synonym)|uniprotkb:Nogo-B receptor(gene name synonym)|uniprotkb:Cis-prenyltransferase subunit NgBR(gene name synonym)	psi-mi:dhdds_human(display_long)|uniprotkb:DHDDS(gene name)|psi-mi:DHDDS(display_short)|uniprotkb:Cis-isoprenyltransferase(gene name synonym)|uniprotkb:Cis-prenyltransferase subunit hCIT(gene name synonym)|uniprotkb:Epididymis tissue protein Li 189m(gene name synonym)|uniprotkb:HDS(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Edani et al. (2020)	pubmed:32817466|imex:IM-28941	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-26971660|imex:IM-28941-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000153989(gene)|ensembl:ENST00000368494(transcript)|go:"GO:0001525"(angiogenesis)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006489"(dolichyl diphosphate biosynthetic process)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019408"(dolichol biosynthetic process)|rcsb pdb:6W2L|rcsb pdb:6Z1N|reactome:R-HSA-446199|reactome:R-HSA-4755609|go:"GO:0030154"(cell differentiation)|go:"GO:0032383"(regulation of intracellular cholesterol transport)|go:"GO:0035268"(protein mannosylation)|go:"GO:0038084"(vascular endothelial growth factor signaling pathway)|go:"GO:0042632"(cholesterol homeostasis)|go:"GO:0043536"(positive regulation of blood vessel endothelial cell migration)|go:"GO:0045547"(dehydrodolichyl diphosphate synthase activity)|go:"GO:0046872"(metal ion binding)|go:"GO:0051000"(positive regulation of nitric-oxide synthase activity)|go:"GO:1904423"(dehydrodolichyl diphosphate synthase complex)|interpro:IPR001441(Di-trans-poly-cis-decaprenylcistransferase)|interpro:IPR036424|interpro:IPR038887|refseq:NP_612468.1|dip:DIP-61225N	refseq:XP_016857869.1|refseq:NP_001230493.1|refseq:NP_001230494.1|refseq:NP_079163.2|refseq:NP_995583.1|refseq:XP_006710975.1|refseq:XP_006710976.1|refseq:XP_006710977.1|refseq:XP_011540485.1|refseq:XP_011540486.1|refseq:XP_011540488.1|refseq:XP_016857868.1|ensembl:ENSG00000117682(gene)|ensembl:ENST00000236342(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0006489"(dolichyl diphosphate biosynthetic process)|go:"GO:0016094"(polyprenol biosynthetic process)|go:"GO:0045547"(dehydrodolichyl diphosphate synthase activity)|go:"GO:0046872"(metal ion binding)|go:"GO:1904423"(dehydrodolichyl diphosphate synthase complex)|interpro:IPR001441(Di-trans-poly-cis-decaprenylcistransferase)|interpro:IPR018520|interpro:IPR036424|rcsb pdb:6W2L|rcsb pdb:6Z1N|reactome:R-HSA-446199|reactome:R-HSA-4755609	-	comment:mint|function:Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator. Able to regulate vascular remodeling and angiogenesis. Its similarity with UPP synthetase proteins suggests that it may act as a scaffold for the binding of isoprenyl lipids and/or prenylated proteins|comment:homomint	-	figure legend:Fig. 1B|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2021/05/07	2021/05/07	rogid:htBbXzyI2bJDFvyuFFWIeAnF3sc9606	rogid:Ernk6WWm+mdwwms7pvGchWbmPfE9606	rigid:MMv8aSQKG+PL02D9HCGYXAQAAkM	false	sufficient binding region:80-293	sufficient binding region:2-330	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:Q96E22	uniprotkb:Q86SQ9	intact:EBI-6949352|ensembl:ENSP00000357480|uniprotkb:O00251|intact:MINT-3052208|uniprotkb:B2RWQ4	intact:EBI-26942900|uniprotkb:B7Z4B9|uniprotkb:B7ZB20|uniprotkb:D3DPK7|uniprotkb:D3DPK8|uniprotkb:D3DPK9|uniprotkb:E9KL43|uniprotkb:Q5T0A4|uniprotkb:Q8NE90|uniprotkb:Q9BTG5|uniprotkb:Q9BTK3|uniprotkb:Q9H905|ensembl:ENSP00000236342	psi-mi:ngbr_human(display_long)|uniprotkb:NUS1(gene name)|psi-mi:NUS1(display_short)|uniprotkb:C6orf68(gene name synonym)|uniprotkb:NGBR(gene name synonym)|uniprotkb:Nuclear undecaprenyl pyrophosphate synthase 1 homolog(gene name synonym)|uniprotkb:Nogo-B receptor(gene name synonym)|uniprotkb:Cis-prenyltransferase subunit NgBR(gene name synonym)	psi-mi:dhdds_human(display_long)|uniprotkb:DHDDS(gene name)|psi-mi:DHDDS(display_short)|uniprotkb:Cis-isoprenyltransferase(gene name synonym)|uniprotkb:Cis-prenyltransferase subunit hCIT(gene name synonym)|uniprotkb:Epididymis tissue protein Li 189m(gene name synonym)|uniprotkb:HDS(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Edani et al. (2020)	pubmed:32817466|imex:IM-28941	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0486"(UniProt)	intact:EBI-26971684|imex:IM-28941-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000153989(gene)|ensembl:ENST00000368494(transcript)|go:"GO:0001525"(angiogenesis)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006489"(dolichyl diphosphate biosynthetic process)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019408"(dolichol biosynthetic process)|rcsb pdb:6W2L|rcsb pdb:6Z1N|reactome:R-HSA-446199|reactome:R-HSA-4755609|go:"GO:0030154"(cell differentiation)|go:"GO:0032383"(regulation of intracellular cholesterol transport)|go:"GO:0035268"(protein mannosylation)|go:"GO:0038084"(vascular endothelial growth factor signaling pathway)|go:"GO:0042632"(cholesterol homeostasis)|go:"GO:0043536"(positive regulation of blood vessel endothelial cell migration)|go:"GO:0045547"(dehydrodolichyl diphosphate synthase activity)|go:"GO:0046872"(metal ion binding)|go:"GO:0051000"(positive regulation of nitric-oxide synthase activity)|go:"GO:1904423"(dehydrodolichyl diphosphate synthase complex)|interpro:IPR001441(Di-trans-poly-cis-decaprenylcistransferase)|interpro:IPR036424|interpro:IPR038887|refseq:NP_612468.1|dip:DIP-61225N	refseq:XP_016857869.1|refseq:NP_001230493.1|refseq:NP_001230494.1|refseq:NP_079163.2|refseq:NP_995583.1|refseq:XP_006710975.1|refseq:XP_006710976.1|refseq:XP_006710977.1|refseq:XP_011540485.1|refseq:XP_011540486.1|refseq:XP_011540488.1|refseq:XP_016857868.1|ensembl:ENSG00000117682(gene)|ensembl:ENST00000236342(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0006489"(dolichyl diphosphate biosynthetic process)|go:"GO:0016094"(polyprenol biosynthetic process)|go:"GO:0045547"(dehydrodolichyl diphosphate synthase activity)|go:"GO:0046872"(metal ion binding)|go:"GO:1904423"(dehydrodolichyl diphosphate synthase complex)|interpro:IPR001441(Di-trans-poly-cis-decaprenylcistransferase)|interpro:IPR018520|interpro:IPR036424|rcsb pdb:6W2L|rcsb pdb:6Z1N|reactome:R-HSA-446199|reactome:R-HSA-4755609	-	comment:mint|function:Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator. Able to regulate vascular remodeling and angiogenesis. Its similarity with UPP synthetase proteins suggests that it may act as a scaffold for the binding of isoprenyl lipids and/or prenylated proteins|comment:homomint	-	figure legend:Fig.2C|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2021/05/07	2021/05/17	rogid:htBbXzyI2bJDFvyuFFWIeAnF3sc9606	rogid:Ernk6WWm+mdwwms7pvGchWbmPfE9606	rigid:MMv8aSQKG+PL02D9HCGYXAQAAkM	false	mutation with no effect:243-243|mutation with no effect:248-248|mutation decreasing interaction strength:252-252|mutation disrupting interaction strength:253-253|mutation disrupting interaction strength:255-255|mutation with no effect:260-260|mutation with no effect:263-263|mutation with no effect:292-292|ha tag:c-c|mutation with no effect:180-180|mutation decreasing interaction strength:196-196|mutation decreasing interaction strength:197-197|mutation disrupting interaction strength:200-200|mutation disrupting interaction strength:226-226|mutation decreasing interaction strength:230-230|mutation with no effect:238-238	flag tag:n-n	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
chebi:"CHEBI:175763"	rhea:"RHEA:53008"	intact:EBI-26972431	intact:EBI-26971796|reactome:R-HSA-6806947|wwpdb:6Z1N|intenz:2.5.1.87|wwpdb:6w2l	psi-mi:"2-trans,6-trans-farnesyl diphosphate(3-)"(display_short)|psi-mi:"CHEBI:175763"(display_long)|intact:"(2-trans,6-trans)-farnesyl diphosphate(3-)"(synonym)|intact:"(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl (phosphonatooxy)phosphonate"(synonym)|intact:"(2E,6E)-farnesyl diphosphate"(synonym)|intact:"(2E,6E)-farnesyl diphosphate(3-)"(synonym)|intact:"(E,E)-farnesyl diphosphate(3-)"(synonym)|intact:"farnesyl pyrophosphate(3-)"(synonym)|intact:"trans,trans-farnesyl diphosphate(3-)"(synonym)|intact:"(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl diphosphate"(iupac name)	psi-mi:dds_human(display_short)|psi-mi:"RHEA:53008"(display_long)|intact:Cis-PTase complex(complex synonym)|intact:NgBR-DHDDS complex(complex synonym)|intact:"2xDHDDS:2xNUS1"(complex systematic name)|intact:Cis-IPTase complex(complex synonym)|intact:Dehydrodolichyl diphosphate synthase complex(complex recommended name)|intact:DDS complex(complex synonym)|intact:Cis-prenyltransferase complex(complex synonym)	psi-mi:"MI:0415"(enzymatic study)	Edani et al. (2020)	pubmed:32817466|imex:IM-28941	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-26972427|imex:IM-28941-7	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	go:"GO:1904423"(dehydrodolichyl diphosphate synthase complex)|pubmed:32817466(see-also)|efo:"MONDO:0044326"(see-also)|efo:"EFO:0005545"(see-also)|efo:"Orphanet:488613"(see-also)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|efo:"Orphanet:791"(see-also)|go:"GO:0006489"(dolichyl diphosphate biosynthetic process)|go:"GO:0045547"(dehydrodolichyl diphosphate synthase activity)|intact:EBI-26971622(exp-evidence)|complex portal:CPX-6701(complex-primary)|evidence ontology:"ECO:0000353"|pubmed:25066056(see-also)|pubmed:33077723(see-also)	go:"GO:0045547"(dehydrodolichyl diphosphate synthase activity)	-	curated-complex:"A cis-prenyltransferase which catalyzes the rate-limiting step in the synthesis of glycosyl carrier lipids required for protein glycosylation in the lumen of endoplasmic reticulum. Essential for the synthesis of dolichol phosphate (CHEBI:23875), an indispensable lipid carrier for protein N-glycosylation, O-mannosylation, C-mannosylation, and GPI-anchor formation, catalyzing the formation of its precursor dehydrodolichyl diphosphate (ditrans,polycis-polyprenyl diphosphate(3-), CHEBI:136960), a long-chain isoprenoid, by chain elongation of farnesyl diphosphate (CHEBI:175763) via multiple condensations with isopentenyl pyrophosphate (CHEBI:128769)."|complex-properties:The DHDDS-NUS1 heterodimer is formed through a large interaction interface with the C-terminus of NUS1 which contributes to enzymatic activity through its C-terminal -RXG- motif, extending across the dimerization interface. The heterotetrameric assembly is formed via a dimer-of-heterodimers mechanism, mainly through homotypic interface formation between the C-terminal helix-turn-helix motifs of DHDDS, and interaction between this motif and the NUS1 protein in the adjoining heterodimer. NUS1 lacks catalytic activity due to an absence of substrate binding sites.|complex-assembly:Heterotetramer|disease:"Retinitis pigmentosa (RP) [Orphanet:791]: an inherited retinal dystrophy leading to progressive loss of the photoreceptors and retinal pigment epithelium and resulting in blindness usually after several decades."|disease:"Congenital disorder of glycosylation type I [EFO:0005545]: A congenital disorder of glycosylation involve disrupted synthesis of the lipid-linked oligosaccharide precursor."|disease:"Developmental delay and seizures with or without movement abnormalities (DEDSM) [MONDO:0044326]: a neurodevelopmental disorder characterized by global developmental delay, variable intellectual disability, and early-onset seizures with a myoclonic component. Most patients have delayed motor development and show abnormal movements, including ataxia, dystonia, and tremor."|disease:"Global developmental delay - neuro-ophthalmological abnormalities - seizures - intellectual disability syndrome [Orphanet:488613]: a form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRD55 patients suffer from seizures appearing during the first years of life."	figure legend:Figs.1C,  4C, 5D|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/05/07	2021/05/18	-	-	-	false	-	mutation decreasing interaction rate:91-91|mutation disrupting interaction strength:12-12,15-15,19-19	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:175763"	rhea:"RHEA:53008"	intact:EBI-26972431	intact:EBI-26971796|reactome:R-HSA-6806947|wwpdb:6Z1N|intenz:2.5.1.87|wwpdb:6w2l	psi-mi:"2-trans,6-trans-farnesyl diphosphate(3-)"(display_short)|psi-mi:"CHEBI:175763"(display_long)|intact:"(2-trans,6-trans)-farnesyl diphosphate(3-)"(synonym)|intact:"(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl (phosphonatooxy)phosphonate"(synonym)|intact:"(2E,6E)-farnesyl diphosphate"(synonym)|intact:"(2E,6E)-farnesyl diphosphate(3-)"(synonym)|intact:"(E,E)-farnesyl diphosphate(3-)"(synonym)|intact:"farnesyl pyrophosphate(3-)"(synonym)|intact:"trans,trans-farnesyl diphosphate(3-)"(synonym)|intact:"(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl diphosphate"(iupac name)	psi-mi:dds_human(display_short)|psi-mi:"RHEA:53008"(display_long)|intact:Cis-PTase complex(complex synonym)|intact:NgBR-DHDDS complex(complex synonym)|intact:"2xDHDDS:2xNUS1"(complex systematic name)|intact:Cis-IPTase complex(complex synonym)|intact:Dehydrodolichyl diphosphate synthase complex(complex recommended name)|intact:DDS complex(complex synonym)|intact:Cis-prenyltransferase complex(complex synonym)	psi-mi:"MI:0415"(enzymatic study)	Edani et al. (2020)	pubmed:32817466|imex:IM-28941	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-26976019|imex:IM-28941-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	go:"GO:1904423"(dehydrodolichyl diphosphate synthase complex)|pubmed:32817466(see-also)|efo:"MONDO:0044326"(see-also)|efo:"EFO:0005545"(see-also)|efo:"Orphanet:488613"(see-also)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|efo:"Orphanet:791"(see-also)|go:"GO:0006489"(dolichyl diphosphate biosynthetic process)|go:"GO:0045547"(dehydrodolichyl diphosphate synthase activity)|intact:EBI-26971622(exp-evidence)|complex portal:CPX-6701(complex-primary)|evidence ontology:"ECO:0000353"|pubmed:25066056(see-also)|pubmed:33077723(see-also)	go:"GO:0045547"(dehydrodolichyl diphosphate synthase activity)	-	curated-complex:"A cis-prenyltransferase which catalyzes the rate-limiting step in the synthesis of glycosyl carrier lipids required for protein glycosylation in the lumen of endoplasmic reticulum. Essential for the synthesis of dolichol phosphate (CHEBI:23875), an indispensable lipid carrier for protein N-glycosylation, O-mannosylation, C-mannosylation, and GPI-anchor formation, catalyzing the formation of its precursor dehydrodolichyl diphosphate (ditrans,polycis-polyprenyl diphosphate(3-), CHEBI:136960), a long-chain isoprenoid, by chain elongation of farnesyl diphosphate (CHEBI:175763) via multiple condensations with isopentenyl pyrophosphate (CHEBI:128769)."|complex-properties:The DHDDS-NUS1 heterodimer is formed through a large interaction interface with the C-terminus of NUS1 which contributes to enzymatic activity through its C-terminal -RXG- motif, extending across the dimerization interface. The heterotetrameric assembly is formed via a dimer-of-heterodimers mechanism, mainly through homotypic interface formation between the C-terminal helix-turn-helix motifs of DHDDS, and interaction between this motif and the NUS1 protein in the adjoining heterodimer. NUS1 lacks catalytic activity due to an absence of substrate binding sites.|complex-assembly:Heterotetramer|disease:"Retinitis pigmentosa (RP) [Orphanet:791]: an inherited retinal dystrophy leading to progressive loss of the photoreceptors and retinal pigment epithelium and resulting in blindness usually after several decades."|disease:"Congenital disorder of glycosylation type I [EFO:0005545]: A congenital disorder of glycosylation involve disrupted synthesis of the lipid-linked oligosaccharide precursor."|disease:"Developmental delay and seizures with or without movement abnormalities (DEDSM) [MONDO:0044326]: a neurodevelopmental disorder characterized by global developmental delay, variable intellectual disability, and early-onset seizures with a myoclonic component. Most patients have delayed motor development and show abnormal movements, including ataxia, dystonia, and tremor."|disease:"Global developmental delay - neuro-ophthalmological abnormalities - seizures - intellectual disability syndrome [Orphanet:488613]: a form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRD55 patients suffer from seizures appearing during the first years of life."	figure legend:Fig.1C, 5D|comment:The activity of the NgBR/DHDDS complex was potently stimulated by phosphatidylinositol|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/05/07	2021/05/18	-	-	-	false	-	mutation decreasing interaction rate:91-91|mutation decreasing interaction rate:15-15,12-12,19-19	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:175763"	chebi:"CHEBI:28874"	intact:EBI-26972431	intact:EBI-2878872|chebi:"CHEBI:18877"|chebi:"CHEBI:494"	psi-mi:"2-trans,6-trans-farnesyl diphosphate(3-)"(display_short)|psi-mi:"CHEBI:175763"(display_long)|intact:"(2-trans,6-trans)-farnesyl diphosphate(3-)"(synonym)|intact:"(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl (phosphonatooxy)phosphonate"(synonym)|intact:"(2E,6E)-farnesyl diphosphate"(synonym)|intact:"(2E,6E)-farnesyl diphosphate(3-)"(synonym)|intact:"(E,E)-farnesyl diphosphate(3-)"(synonym)|intact:"farnesyl pyrophosphate(3-)"(synonym)|intact:"trans,trans-farnesyl diphosphate(3-)"(synonym)|intact:"(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl diphosphate"(iupac name)	psi-mi:pi(display_short)|psi-mi:"CHEBI:28874"(display_long)|intact:phosphatidylinositols(synonym)|intact:PI(synonym)|intact:PtdIns(synonym)	psi-mi:"MI:0415"(enzymatic study)	Edani et al. (2020)	pubmed:32817466|imex:IM-28941	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-26976019|imex:IM-28941-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:1343"(enzyme regulator)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0328"(small molecule)	-	-	go:"GO:0045547"(dehydrodolichyl diphosphate synthase activity)	-	-	figure legend:Fig.1C, 5D|comment:The activity of the NgBR/DHDDS complex was potently stimulated by phosphatidylinositol|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/05/07	2021/05/18	-	-	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)