#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q8K1M6-3	uniprotkb:P61158	intact:EBI-16092613|ensembl:ENSMUSP00000023477	intact:EBI-351428|uniprotkb:P32391|ensembl:ENSP00000263238|uniprotkb:Q53QM2	psi-mi:q8k1m6-3(display_long)|uniprotkb:Dnm1l(gene name)|psi-mi:Dnm1l(display_short)|uniprotkb:Drp1(gene name synonym)|uniprotkb:Dynamin family member proline-rich carboxyl-terminal domain less(gene name synonym)|uniprotkb:Dynamin-related protein 1(gene name synonym)	psi-mi:arp3_human(display_long)|uniprotkb:ACTR3(gene name)|psi-mi:ACTR3(display_short)|uniprotkb:ARP3(gene name synonym)|uniprotkb:Actin-like protein 3(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Galvan et al. (2019)	imex:IM-28850|pubmed:31063459	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26880519|imex:IM-28850-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-2365792(isoform-parent)|ensembl:ENSMUSG00000022789(gene)|ensembl:ENSMUST00000023477(transcript)	refseq:NP_005712.1|ensembl:ENSG00000115091(gene)|ensembl:ENST00000263238(transcript)|go:"GO:0003779"(actin binding)|go:"GO:0005200"(structural constituent of cytoskeleton)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005885"(Arp2/3 protein complex)|go:"GO:0005903"(brush border)|go:"GO:0005911"(cell-cell junction)|go:"GO:0005925"(focal adhesion)|go:"GO:0007163"(establishment or maintenance of cell polarity)|go:"GO:0008356"(asymmetric cell division)|go:"GO:0010592"(positive regulation of lamellipodium assembly)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0016020"(membrane)|go:"GO:0016344"(meiotic chromosome movement towards spindle pole)|go:"GO:0030027"(lamellipodium)|go:"GO:0033206"(meiotic cytokinesis)|go:"GO:0034314"(Arp2/3 complex-mediated actin nucleation)|go:"GO:0035861"(site of double-strand break)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0051653"(spindle localization)|go:"GO:0060271"(cilium assembly)|go:"GO:0070062"(extracellular exosome)|go:"GO:0070358"(actin polymerization-dependent cell motility)|go:"GO:0071346"(cellular response to interferon-gamma)|interpro:IPR004000(Actin/actin-like)|interpro:IPR015623(Actin-related protein 3)|interpro:IPR020902|interpro:IPR043129|mint:P61158|rcsb pdb:6UHC|rcsb pdb:6YW6|rcsb pdb:6YW7|reactome:R-HSA-2029482|reactome:R-HSA-3928662|reactome:R-HSA-5663213|reactome:R-HSA-8856828|reactome:R-HSA-9664422|dip:DIP-33140N	-	-	-	figure legend:Fig. 7A|comment:"To this end, we transfected GFP-tagged Arp3 into cultured podocytes stably expressing empty vector control, FLAG-tagged WT Drp1S600, or Drp1S600 mutants (S600A or S600D). Western blot detection of the immunoprecipitated FLAG-tagged proteins indicted that the interaction of Drp1 with the GFP-Arp3 complex was markedly increased in cells that were cultured in HG conditions and in Drp1S600D-transfected podocytes (Figure 7A). However, this interaction was markedly reduced in FLAG-Drp1S600A cells under HG conditions, suggesting that, indeed, the binding of Drp1 to the Arp3 complex was enhanced by the phosphorylation of Drp1S600."|agonist:"high-glucose (CHEBI:17234)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-podocyte)|taxid:10090(Mus musculus)	-	2021/03/18	2021/03/18	rogid:ZVS7ZScJZXzokKQo3oAY+qqf3YU10090	rogid:XbkZeKzG8lGbT8dxYkGqeJT0vEg9606	rigid:BU4yD12FWq6ls2m5Tfvy/9fAFqE	false	flag tag:?-?|mutation decreasing interaction strength:600-600|mutation increasing interaction strength:600-600	green fluorescent protein tag:c-c	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q8K1M6-3	uniprotkb:P23528	intact:EBI-16092613|ensembl:ENSMUSP00000023477	intact:EBI-352733|uniprotkb:Q53Y87|uniprotkb:Q9UCA2|uniprotkb:B3KUQ1|ensembl:ENSP00000309629|ensembl:ENSP00000432660	psi-mi:q8k1m6-3(display_long)|uniprotkb:Dnm1l(gene name)|psi-mi:Dnm1l(display_short)|uniprotkb:Drp1(gene name synonym)|uniprotkb:Dynamin family member proline-rich carboxyl-terminal domain less(gene name synonym)|uniprotkb:Dynamin-related protein 1(gene name synonym)	psi-mi:cof1_human(display_long)|uniprotkb:CFL1(gene name)|psi-mi:CFL1(display_short)|uniprotkb:CFL(gene name synonym)|uniprotkb:Cofilin, non-muscle isoform(gene name synonym)|uniprotkb:18 kDa phosphoprotein(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Galvan et al. (2019)	imex:IM-28850|pubmed:31063459	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26880578|imex:IM-28850-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-2365792(isoform-parent)|ensembl:ENSMUSG00000022789(gene)|ensembl:ENSMUST00000023477(transcript)	refseq:NP_005498.1|ensembl:ENSG00000172757(gene)|go:"GO:0031982"(vesicle)|go:"GO:0032587"(ruffle membrane)|go:"GO:0040019"(positive regulation of embryonic development)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0044794"(positive regulation by host of viral process)|go:"GO:0048870"(cell motility)|go:"GO:0051014"(actin filament severing)|go:"GO:0051015"(actin filament binding)|go:"GO:0051293"(establishment of spindle localization)|go:"GO:0061001"(regulation of dendritic spine morphogenesis)|go:"GO:0070062"(extracellular exosome)|interpro:IPR002108(Actin-binding, cofilin/tropomyosin type)|interpro:IPR017904|interpro:IPR027234|interpro:IPR029006|mint:P23528|rcsb pdb:1Q8G|rcsb pdb:1Q8X|rcsb pdb:3J0S|rcsb pdb:4BEX|rcsb pdb:5HVK|rcsb pdb:5L6W|rcsb pdb:6UBY|rcsb pdb:6UC0|rcsb pdb:6UC4|rcsb pdb:6VAO|reactome:R-HSA-114608|reactome:R-HSA-2029482|reactome:R-HSA-3928662|reactome:R-HSA-399954|reactome:R-HSA-5627117|reactome:R-HSA-8950505|ensembl:ENST00000308162(transcript)|ensembl:ENST00000525451(transcript)|go:"GO:0005615"(extracellular space)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005925"(focal adhesion)|go:"GO:0007010"(cytoskeleton organization)|go:"GO:0007266"(Rho protein signal transduction)|go:"GO:0009615"(response to virus)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0016020"(membrane)|go:"GO:0016363"(nuclear matrix)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030027"(lamellipodium)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030042"(actin filament depolymerization)|go:"GO:0030043"(actin filament fragmentation)|go:"GO:0031258"(lamellipodium membrane)|dip:DIP-33000N	-	-	-	figure legend:Fig. S11C|agonist:"high-glucose (CHEBI:17234)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-podocyte)|taxid:10090(Mus musculus)	-	2021/03/18	2021/03/18	rogid:ZVS7ZScJZXzokKQo3oAY+qqf3YU10090	rogid:wgBEUL55sE9G9Be1f/A1OA6KRb49606	rigid:AFJl9fmcQqaRzgqo+qYvYHIm24o	false	flag tag:?-?|mutation decreasing interaction strength:600-600|mutation increasing interaction strength:600-600	green fluorescent protein tag:c-c	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q8K1M6-3	uniprotkb:A0A287A1D1	intact:EBI-16092613|ensembl:ENSMUSP00000023477	intact:EBI-26880556|ensembl:ENSSSCP00000037561	psi-mi:q8k1m6-3(display_long)|uniprotkb:Dnm1l(gene name)|psi-mi:Dnm1l(display_short)|uniprotkb:Drp1(gene name synonym)|uniprotkb:Dynamin family member proline-rich carboxyl-terminal domain less(gene name synonym)|uniprotkb:Dynamin-related protein 1(gene name synonym)	psi-mi:a0a287a1d1_pig(display_long)|uniprotkb:ARPC2(gene name)|psi-mi:ARPC2(display_short)	psi-mi:"MI:0096"(pull down)	Galvan et al. (2019)	imex:IM-28850|pubmed:31063459	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9823(pig)|taxid:9823("Sus scrofa (Pig)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26880540|imex:IM-28850-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-2365792(isoform-parent)|ensembl:ENSMUSG00000022789(gene)|ensembl:ENSMUST00000023477(transcript)	ensembl:ENSSSCG00000016185(gene)|ensembl:ENSSSCT00000064525(transcript)|go:"GO:0005200"(structural constituent of cytoskeleton)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005768"(endosome)|go:"GO:0005885"(Arp2/3 protein complex)|go:"GO:0005925"(focal adhesion)|go:"GO:0010592"(positive regulation of lamellipodium assembly)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0034314"(Arp2/3 complex-mediated actin nucleation)|go:"GO:0036195"(muscle cell projection membrane)|go:"GO:0043005"(neuron projection)|go:"GO:0051015"(actin filament binding)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1900026"(positive regulation of substrate adhesion-dependent cell spreading)|interpro:IPR007188(Arp2/3 complex, 34kDa subunit p34-Arc)|interpro:IPR034666|reactome:R-SSC-2029482|reactome:R-SSC-5663213|reactome:R-SSC-8856828	-	-	-	figure legend:Fig. 7B|comment:"We found enhanced recovery of both Arp2 and Arp3 in GST-Drp1S600D beads but not in GST-Drp1S600A beads (Figure 7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/03/18	2021/03/18	rogid:ZVS7ZScJZXzokKQo3oAY+qqf3YU10090	rogid:P8/7LVEGYAW08CPY3nVekDiFcK49823	rigid:87GA+fNS3ru26EZiRpw9fAsd7Zc	false	glutathione s tranferase tag:?-?|mutation with no effect:600-600|mutation increasing interaction strength:600-600	-	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q8K1M6-3	uniprotkb:F2Z5D2	intact:EBI-16092613|ensembl:ENSMUSP00000023477	intact:EBI-6170845|ensembl:ENSSSCP00000016650	psi-mi:q8k1m6-3(display_long)|uniprotkb:Dnm1l(gene name)|psi-mi:Dnm1l(display_short)|uniprotkb:Drp1(gene name synonym)|uniprotkb:Dynamin family member proline-rich carboxyl-terminal domain less(gene name synonym)|uniprotkb:Dynamin-related protein 1(gene name synonym)	psi-mi:f2z5d2_pig(display_long)|uniprotkb:ACTR3(gene name)|psi-mi:ACTR3(display_short)|uniprotkb:Actin-related protein 3(gene name synonym)	psi-mi:"MI:0096"(pull down)	Galvan et al. (2019)	imex:IM-28850|pubmed:31063459	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9823(pig)|taxid:9823("Sus scrofa (Pig)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-26880540|imex:IM-28850-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-2365792(isoform-parent)|ensembl:ENSMUSG00000022789(gene)|ensembl:ENSMUST00000023477(transcript)	ensembl:ENSSSCG00000015710(gene)|ensembl:ENSSSCT00000017110(transcript)|go:"GO:0005200"(structural constituent of cytoskeleton)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005885"(Arp2/3 protein complex)|go:"GO:0005903"(brush border)|go:"GO:0005911"(cell-cell junction)|go:"GO:0007163"(establishment or maintenance of cell polarity)|go:"GO:0005737"(cytoplasm)|go:"GO:0008356"(asymmetric cell division)|go:"GO:0010592"(positive regulation of lamellipodium assembly)|go:"GO:0016344"(meiotic chromosome movement towards spindle pole)|go:"GO:0030027"(lamellipodium)|go:"GO:0033206"(meiotic cytokinesis)|go:"GO:0034314"(Arp2/3 complex-mediated actin nucleation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0051015"(actin filament binding)|go:"GO:0051653"(spindle localization)|go:"GO:0060271"(cilium assembly)|go:"GO:0071346"(cellular response to interferon-gamma)|interpro:IPR004000(Actin/actin-like)|interpro:IPR015623(Actin-related protein 3)|interpro:IPR020902|interpro:IPR043129|reactome:R-SSC-2029482|reactome:R-SSC-5663213|reactome:R-SSC-8856828	-	-	-	figure legend:Fig. 7B|comment:"We found enhanced recovery of both Arp2 and Arp3 in GST-Drp1S600D beads but not in GST-Drp1S600A beads (Figure 7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2021/03/18	2021/03/18	rogid:ZVS7ZScJZXzokKQo3oAY+qqf3YU10090	rogid:0rR57LpqnQmftIHcJU2u+As8C6U9823	rigid:87GA+fNS3ru26EZiRpw9fAsd7Zc	false	glutathione s tranferase tag:?-?|mutation with no effect:600-600|mutation increasing interaction strength:600-600	-	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)