#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q9QUK6	uniprotkb:P63038	intact:EBI-1534575|uniprotkb:Q9D691|uniprotkb:Q9QZF5|uniprotkb:Q9Z203|ensembl:ENSMUSP00000045770	intact:EBI-299343|ensembl:ENSMUSP00000027123|uniprotkb:P19226|uniprotkb:P19227|uniprotkb:P97602|uniprotkb:Q3KQP2|uniprotkb:Q3UIP0|uniprotkb:Q8C2C7|uniprotkb:Q8VEF4	psi-mi:tlr4_mouse(display_long)|uniprotkb:Tlr4(gene name)|psi-mi:Tlr4(display_short)|uniprotkb:Lps(gene name synonym)	psi-mi:ch60_mouse(display_long)|uniprotkb:Hspd1(gene name)|psi-mi:Hspd1(display_short)|uniprotkb:Hsp60(gene name synonym)|uniprotkb:Heat shock protein 60(gene name synonym)|uniprotkb:60 kDa chaperonin(gene name synonym)|uniprotkb:Chaperonin 60(gene name synonym)|uniprotkb:Mitochondrial matrix protein P1(gene name synonym)|uniprotkb:HSP-65(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Swaroop et al. (2016)	imex:IM-29119|pubmed:26838598	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-27047744|imex:IM-29119-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_067272.1|dip:DIP-38573N|ensembl:ENSMUSG00000039005(gene)|ensembl:ENSMUST00000048096(transcript)|go:"GO:0001530"(lipopolysaccharide binding)|go:"GO:0001726"(ruffle)|go:"GO:0001774"(microglial cell activation)|go:"GO:0001875"(lipopolysaccharide immune receptor activity)|go:"GO:0001891"(phagocytic cup)|go:"GO:0002218"(activation of innate immune response)|go:"GO:0002224"(toll-like receptor signaling pathway)|go:"GO:0002322"(B cell proliferation involved in immune response)|go:"GO:0002537"(nitric oxide production involved in inflammatory response)|go:"GO:0002730"(regulation of dendritic cell cytokine production)|go:"GO:0002755"(MyD88-dependent toll-like receptor signaling pathway)|go:"GO:0002758"(innate immune response-activating signal transduction)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005769"(early endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006691"(leukotriene metabolic process)|go:"GO:0006909"(phagocytosis)|go:"GO:0006954"(inflammatory response)|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007250"(activation of NF-kappaB-inducing kinase activity)|go:"GO:0007252"(I-kappaB phosphorylation)|go:"GO:0009617"(response to bacterium)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0010572"(positive regulation of platelet activation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0014002"(astrocyte development)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0031226"(intrinsic component of plasma membrane)|go:"GO:0031663"(lipopolysaccharide-mediated signaling pathway)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032497"(detection of lipopolysaccharide)|go:"GO:0032680"(regulation of tumor necrosis factor production)|go:"GO:0032689"(negative regulation of interferon-gamma production)|go:"GO:0032700"(negative regulation of interleukin-17 production)|go:"GO:0032707"(negative regulation of interleukin-23 production)|go:"GO:0032715"(negative regulation of interleukin-6 production)|go:"GO:0032720"(negative regulation of tumor necrosis factor production)|go:"GO:0032722"(positive regulation of chemokine production)|go:"GO:0032727"(positive regulation of interferon-alpha production)|go:"GO:0032728"(positive regulation of interferon-beta production)|go:"GO:0032729"(positive regulation of interferon-gamma production)|go:"GO:0032731"(positive regulation of interleukin-1 beta production)|go:"GO:0032732"(positive regulation of interleukin-1 production)|go:"GO:0032733"(positive regulation of interleukin-10 production)|go:"GO:0032735"(positive regulation of interleukin-12 production)|go:"GO:0032736"(positive regulation of interleukin-13 production)|go:"GO:0032755"(positive regulation of interleukin-6 production)|go:"GO:0032757"(positive regulation of interleukin-8 production)|go:"GO:0032760"(positive regulation of tumor necrosis factor production)|go:"GO:0032874"(positive regulation of stress-activated MAPK cascade)|go:"GO:0034142"(toll-like receptor 4 signaling pathway)|go:"GO:0035666"(TRIF-dependent toll-like receptor signaling pathway)|go:"GO:0038023"(signaling receptor activity)|go:"GO:0042116"(macrophage activation)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043032"(positive regulation of macrophage activation)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043123"(positive regulation of I-kappaB kinase/NF-kappaB signaling)|go:"GO:0043235"(receptor complex)|go:"GO:0043388"(positive regulation of DNA binding)|go:"GO:0043406"(positive regulation of MAP kinase activity)|go:"GO:0043548"(phosphatidylinositol 3-kinase binding)|go:"GO:0045087"(innate immune response)|go:"GO:0045348"(positive regulation of MHC class II biosynthetic process)|go:"GO:0045429"(positive regulation of nitric oxide biosynthetic process)|go:"GO:0045471"(response to ethanol)|go:"GO:0045576"(mast cell activation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046330"(positive regulation of JNK cascade)|go:"GO:0046696"(lipopolysaccharide receptor complex)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050135"("NAD(P)+ nucleosidase activity")|go:"GO:0050671"(positive regulation of lymphocyte proliferation)|go:"GO:0050727"(regulation of inflammatory response)|go:"GO:0050729"(positive regulation of inflammatory response)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050965"(detection of temperature stimulus involved in sensory perception of pain)|go:"GO:0050966"(detection of mechanical stimulus involved in sensory perception of pain)|go:"GO:0051091"(positive regulation of DNA-binding transcription factor activity)|go:"GO:0051092"(positive regulation of NF-kappaB transcription factor activity)|go:"GO:0051770"(positive regulation of nitric-oxide synthase biosynthetic process)|go:"GO:0051930"(regulation of sensory perception of pain)|go:"GO:0060729"(intestinal epithelial structure maintenance)|go:"GO:0060907"(positive regulation of macrophage cytokine production)|go:"GO:0061809"(NAD+ nucleotidase, cyclic ADP-ribose generating)|go:"GO:0070373"(negative regulation of ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0070430"(positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway)|go:"GO:0070434"(positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway)|go:"GO:0070542"(response to fatty acid)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071223"(cellular response to lipoteichoic acid)|go:"GO:0071260"(cellular response to mechanical stimulus)|go:"GO:0071346"(cellular response to interferon-gamma)|go:"GO:0120163"(negative regulation of cold-induced thermogenesis)|go:"GO:0140052"(cellular response to oxidised low-density lipoprotein particle stimulus)|go:"GO:1900017"(positive regulation of cytokine production involved in inflammatory response)|go:"GO:1900227"(positive regulation of NLRP3 inflammasome complex assembly)|go:"GO:1901224"(positive regulation of NIK/NF-kappaB signaling)|go:"GO:1903223"(positive regulation of oxidative stress-induced neuron death)|go:"GO:1903428"(positive regulation of reactive oxygen species biosynthetic process)|go:"GO:1903974"(positive regulation of cellular response to macrophage colony-stimulating factor stimulus)|go:"GO:1904466"(positive regulation of matrix metallopeptidase secretion)|go:"GO:1904646"(cellular response to amyloid-beta)|go:"GO:2000343"("positive regulation of chemokine (C-X-C motif) ligand 2 production")|interpro:IPR000157(Toll-Interleukin receptor)|interpro:IPR000483(Cysteine-rich flanking region, C-terminal)|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR003591(Leucine-rich repeat, typical subtype)|interpro:IPR017241(Toll-like receptor)|interpro:IPR032675|interpro:IPR035897|rcsb pdb:2Z64|rcsb pdb:3VQ1|rcsb pdb:3VQ2|rcsb pdb:5IJB|rcsb pdb:5IJC|rcsb pdb:5IJD|reactome:R-MMU-140534|reactome:R-MMU-166016|reactome:R-MMU-166166|reactome:R-MMU-2562578|reactome:R-MMU-5686938|reactome:R-MMU-936964|reactome:R-MMU-937041|reactome:R-MMU-937072|reactome:R-MMU-9707616|reactome:R-MMU-975163	ensembl:ENSMUSG00000025980(gene)|ensembl:ENSMUST00000027123(transcript)|go:"GO:0001530"(lipopolysaccharide binding)|go:"GO:0001666"(response to hypoxia)|go:"GO:0002039"(p53 binding)|go:"GO:0002755"(MyD88-dependent toll-like receptor signaling pathway)|go:"GO:0002842"(positive regulation of T cell mediated immune response to tumor cell)|go:"GO:0002931"(response to ischemia)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005759"(mitochondrial matrix)|go:"GO:0005769"(early endosome)|go:"GO:0005782"(peroxisomal matrix)|go:"GO:0005791"(rough endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006457"(protein folding)|go:"GO:0006919"(activation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0006986"(response to unfolded protein)|go:"GO:0008035"(high-density lipoprotein particle binding)|go:"GO:0008637"(apoptotic mitochondrial changes)|go:"GO:0009409"(response to cold)|go:"GO:0009986"(cell surface)|go:"GO:0014823"(response to activity)|go:"GO:0016020"(membrane)|go:"GO:0016853"(isomerase activity)|go:"GO:0019899"(enzyme binding)|go:"GO:0030061"(mitochondrial crista)|go:"GO:0030135"(coated vesicle)|go:"GO:0030141"(secretory granule)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032727"(positive regulation of interferon-alpha production)|go:"GO:0032729"(positive regulation of interferon-gamma production)|go:"GO:0032733"(positive regulation of interleukin-10 production)|go:"GO:0032735"(positive regulation of interleukin-12 production)|go:"GO:0032755"(positive regulation of interleukin-6 production)|go:"GO:0032760"(positive regulation of tumor necrosis factor production)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033198"(response to ATP)|go:"GO:0034185"(apolipoprotein binding)|go:"GO:0034186"(apolipoprotein A-I binding)|go:"GO:0034514"(mitochondrial unfolded protein response)|go:"GO:0034605"(cellular response to heat)|go:"GO:0042026"(protein refolding)|go:"GO:0042100"(B cell proliferation)|go:"GO:0042110"(T cell activation)|go:"GO:0042113"(B cell activation)|go:"GO:0042220"(response to cocaine)|go:"GO:0042493"|go:"GO:0042542"(response to hydrogen peroxide)|go:"GO:0042588"(zymogen granule)|go:"GO:0043032"(positive regulation of macrophage activation)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043209"(myelin sheath)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0043627"(response to estrogen)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045041"(protein import into mitochondrial intermembrane space)|go:"GO:0045121"(membrane raft)|go:"GO:0046696"(lipopolysaccharide receptor complex)|go:"GO:0048291"(isotype switching to IgG isotypes)|go:"GO:0050729"(positive regulation of inflammatory response)|go:"GO:0050821"(protein stabilization)|go:"GO:0050870"(positive regulation of T cell activation)|go:"GO:0051087"(chaperone binding)|go:"GO:0051384"(response to glucocorticoid)|go:"GO:0051702"(biological process involved in interaction with symbiont)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071866"(negative regulation of apoptotic process in bone marrow cell)|go:"GO:0097225"(sperm midpiece)|go:"GO:0098761"(cellular response to interleukin-7)|go:"GO:0140030"(modification-dependent protein binding)|go:"GO:1903427"(negative regulation of reactive oxygen species biosynthetic process)|interpro:IPR001844(Chaperonin Cpn60)|interpro:IPR002423(Chaperonin Cpn60/TCP-1)|interpro:IPR018370|interpro:IPR027409|interpro:IPR027410|interpro:IPR027413|mint:P63038|reactome:R-MMU-1268020|refseq:XP_006495769.1|refseq:NP_034607.3|dip:DIP-31396N	-	-	-	figure legend:fig. 4b|agonist:Interleukin-1 beta|comment:"To check whether HSP60 secreted by microglia in response to IL-1β treatment binds with TLR4, we determined the interaction between HSP60 and TLR4 using co-immunoprecipitation technique. Five hundred microgram of N9 microglial cellular extract was precipitated with HSP60 antibody, and the blots were probed for TLR4 as well as for HSP60. We found the expression of TLR4 in the immunoprecipitate that was pulled using HSP60 antibody (Fig. 4b)."|dataset:Brain Disease - Neurological Diseases & Disorders|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-n9)|taxid:10090(mouse n9 cell line)	-	2021/07/27	2021/07/27	rogid:tVbu3ZsGm01Y4tO+vmeNtUXX7rc10090	rogid:WD1fQLh4I0ovpakE8KtM1R0XpL410090	rigid:P8BnnAmSKDUyHCqS+pd2+OQlw0k	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)