#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:Q92643 uniprotkb:O43292 intact:EBI-8617711|ensembl:ENSP00000359848|intact:MINT-2813159|uniprotkb:O14822|uniprotkb:B2R7K3|uniprotkb:Q5TG77|uniprotkb:B4E2M3 intact:EBI-720225|uniprotkb:Q9NSS0|uniprotkb:Q9UQ31|ensembl:ENSP00000347206 psi-mi:gpi8_human(display_long)|uniprotkb:PIGK(gene name)|psi-mi:PIGK(display_short)|uniprotkb:GPI8(gene name synonym)|uniprotkb:Phosphatidylinositol-glycan biosynthesis class K protein(gene name synonym)|uniprotkb:GPI8 homolog(gene name synonym) psi-mi:gpaa1_human(display_long)|uniprotkb:GAA1 protein homolog(gene name synonym)|uniprotkb:GPAA1(gene name)|psi-mi:GPAA1(display_short)|uniprotkb:GAA1(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Ohishi et al. (2000) pubmed:10793132|imex:IM-28799 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-26873638|imex:IM-28799-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSG00000142892(gene)|ensembl:ENST00000370812(transcript)|go:"GO:0003756"(protein disulfide isomerase activity)|go:"GO:0003923"(GPI-anchor transamidase activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016020"(membrane)|go:"GO:0016255"(attachment of GPI anchor to protein)|go:"GO:0034394"(protein localization to cell surface)|go:"GO:0042765"(GPI-anchor transamidase complex)|interpro:IPR001096(Peptidase C13, legumain)|interpro:IPR028361|mint:Q92643|reactome:R-HSA-162791|refseq:NP_005473.1 refseq:NP_003792.1|ensembl:ENSG00000197858(gene)|ensembl:ENST00000355091(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0006508"(proteolysis)|go:"GO:0006621"(protein retention in ER lumen)|go:"GO:0015631"(tubulin binding)|go:"GO:0016020"(membrane)|go:"GO:0016255"(attachment of GPI anchor to protein)|go:"GO:0042765"(GPI-anchor transamidase complex)|go:"GO:0065003"(protein-containing complex assembly)|interpro:IPR007246(Gaa1-like, GPI transamidase component)|mint:O43292|reactome:R-HSA-162791 - function:Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein|comment:mint - figure legend:Fig. 6|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10029(crigr-cho)|taxid:10029(chinese hamster ovary cell line) - 2021/03/09 2021/03/09 rogid:FRRsPTVrMU/nfpydL7D59VLlXxY9606 rogid:rAbNrvIdzfJFRNTZ5qxulRrc/KQ9606 rigid:BHWqnoFGLcsDq/SVCuAHTV/MFHA false glutathione s tranferase tag:c-c flag tag:n-n - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot) uniprotkb:Q92643 uniprotkb:O43292 intact:EBI-8617711|ensembl:ENSP00000359848|intact:MINT-2813159|uniprotkb:O14822|uniprotkb:B2R7K3|uniprotkb:Q5TG77|uniprotkb:B4E2M3 intact:EBI-720225|uniprotkb:Q9NSS0|uniprotkb:Q9UQ31|ensembl:ENSP00000347206 psi-mi:gpi8_human(display_long)|uniprotkb:PIGK(gene name)|psi-mi:PIGK(display_short)|uniprotkb:GPI8(gene name synonym)|uniprotkb:Phosphatidylinositol-glycan biosynthesis class K protein(gene name synonym)|uniprotkb:GPI8 homolog(gene name synonym) psi-mi:gpaa1_human(display_long)|uniprotkb:GAA1 protein homolog(gene name synonym)|uniprotkb:GPAA1(gene name)|psi-mi:GPAA1(display_short)|uniprotkb:GAA1(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Ohishi et al. (2000) pubmed:10793132|imex:IM-28799 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-26873646|imex:IM-28799-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSG00000142892(gene)|ensembl:ENST00000370812(transcript)|go:"GO:0003756"(protein disulfide isomerase activity)|go:"GO:0003923"(GPI-anchor transamidase activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016020"(membrane)|go:"GO:0016255"(attachment of GPI anchor to protein)|go:"GO:0034394"(protein localization to cell surface)|go:"GO:0042765"(GPI-anchor transamidase complex)|interpro:IPR001096(Peptidase C13, legumain)|interpro:IPR028361|mint:Q92643|reactome:R-HSA-162791|refseq:NP_005473.1 refseq:NP_003792.1|ensembl:ENSG00000197858(gene)|ensembl:ENST00000355091(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0006508"(proteolysis)|go:"GO:0006621"(protein retention in ER lumen)|go:"GO:0015631"(tubulin binding)|go:"GO:0016020"(membrane)|go:"GO:0016255"(attachment of GPI anchor to protein)|go:"GO:0042765"(GPI-anchor transamidase complex)|go:"GO:0065003"(protein-containing complex assembly)|interpro:IPR007246(Gaa1-like, GPI transamidase component)|mint:O43292|reactome:R-HSA-162791 - function:Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein|comment:mint - figure legend:Fig. 7c|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10029(crigr-cho)|taxid:10029(chinese hamster ovary cell line) - 2021/03/09 2021/03/09 rogid:FRRsPTVrMU/nfpydL7D59VLlXxY9606 rogid:rAbNrvIdzfJFRNTZ5qxulRrc/KQ9606 rigid:BHWqnoFGLcsDq/SVCuAHTV/MFHA false flag tag:n-n|sufficient binding region:1-310 ha tag:?-? - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot)