#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P0AFM6	uniprotkb:P37344	intact:EBI-1123459|uniprotkb:P23853|uniprotkb:P76040|uniprotkb:P77363|ensemblbacteria:AAC74386|ensemblbacteria:BAA14873	intact:EBI-1123431|uniprotkb:P76039|ensemblbacteria:AAC74385|ensemblbacteria:BAA14872	psi-mi:pspa_ecoli(display_long)|uniprotkb:pspA(gene name)|psi-mi:pspA(display_short)|uniprotkb:b1304(locus name)|uniprotkb:JW1297(locus name)	psi-mi:pspf_ecoli(display_long)|uniprotkb:pspF(gene name)|psi-mi:pspF(display_short)|uniprotkb:b1303(locus name)|uniprotkb:JW1296(locus name)|uniprotkb:ycjB(gene name synonym)|uniprotkb:Phage shock protein F(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Osadnik et al. (2015)	pubmed:26235546|imex:IM-28081	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25567707|imex:IM-28081-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_415820.1|refseq:WP_000511025.1|ensemblbacteria:AAC74386(transcript)|ensemblbacteria:b1304(gene)|ensemblbacteria:BAA14873(gene)|ensemblbacteria:BAA14873(transcript)|go:"GO:0005543"(phospholipid binding)|go:"GO:0005829"(cytosol)|go:"GO:0009271"(phage shock)|go:"GO:0009408"(response to heat)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043433"(negative regulation of DNA-binding transcription factor activity)|go:"GO:0060187"(cell pole)|interpro:IPR007157(PspA/IM30)|interpro:IPR014319(Phage shock protein A)|rcsb pdb:4WHE|dip:DIP-10587N	refseq:NP_415819.4|refseq:WP_001301108.1|ensemblbacteria:AAC74385(transcript)|ensemblbacteria:b1303(gene)|ensemblbacteria:BAA14872(gene)|ensemblbacteria:BAA14872(transcript)|go:"GO:0000160"(phosphorelay signal transduction system)|go:"GO:0003677"(DNA binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|interpro:IPR002078(RNA polymerase sigma factor 54, interaction)|interpro:IPR002197(Helix-turn-helix, Fis-type)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR009057(Homeodomain-like)|interpro:IPR014317(Transcriptional activator, PspF)|interpro:IPR025943|interpro:IPR025944|interpro:IPR027417|rcsb pdb:2BJV|rcsb pdb:2BJW|rcsb pdb:2C96|rcsb pdb:2C98|rcsb pdb:2C99|rcsb pdb:2C9C|rcsb pdb:2VII|rcsb pdb:4QNM|rcsb pdb:4QNR|rcsb pdb:4QOS|rcsb pdb:5NSS|dip:DIP-10592N	-	-	-	figure legend:Fig. 4E, Supp FIg. 4E|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2020/04/21	2020/04/21	rogid:nkBWxxwZtM6XiHcyZqIy0sQLXS883333	rogid:VGlvabOQaaDDNH2dbOlcn/J098c83333	rigid:EivrzR8rPaiJ+DRi25kfNyafBAc	false	his tag:c-c|sufficient binding region:1-144|mutation with no effect:37-37	strep tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:P0AFM6	uniprotkb:P37344	intact:EBI-1123459|uniprotkb:P23853|uniprotkb:P76040|uniprotkb:P77363|ensemblbacteria:AAC74386|ensemblbacteria:BAA14873	intact:EBI-1123431|uniprotkb:P76039|ensemblbacteria:AAC74385|ensemblbacteria:BAA14872	psi-mi:pspa_ecoli(display_long)|uniprotkb:pspA(gene name)|psi-mi:pspA(display_short)|uniprotkb:b1304(locus name)|uniprotkb:JW1297(locus name)	psi-mi:pspf_ecoli(display_long)|uniprotkb:pspF(gene name)|psi-mi:pspF(display_short)|uniprotkb:b1303(locus name)|uniprotkb:JW1296(locus name)|uniprotkb:ycjB(gene name synonym)|uniprotkb:Phage shock protein F(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Osadnik et al. (2015)	pubmed:26235546|imex:IM-28081	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25567762|imex:IM-28081-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_415820.1|refseq:WP_000511025.1|ensemblbacteria:AAC74386(transcript)|ensemblbacteria:b1304(gene)|ensemblbacteria:BAA14873(gene)|ensemblbacteria:BAA14873(transcript)|go:"GO:0005543"(phospholipid binding)|go:"GO:0005829"(cytosol)|go:"GO:0009271"(phage shock)|go:"GO:0009408"(response to heat)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043433"(negative regulation of DNA-binding transcription factor activity)|go:"GO:0060187"(cell pole)|interpro:IPR007157(PspA/IM30)|interpro:IPR014319(Phage shock protein A)|rcsb pdb:4WHE|dip:DIP-10587N	refseq:NP_415819.4|refseq:WP_001301108.1|ensemblbacteria:AAC74385(transcript)|ensemblbacteria:b1303(gene)|ensemblbacteria:BAA14872(gene)|ensemblbacteria:BAA14872(transcript)|go:"GO:0000160"(phosphorelay signal transduction system)|go:"GO:0003677"(DNA binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|interpro:IPR002078(RNA polymerase sigma factor 54, interaction)|interpro:IPR002197(Helix-turn-helix, Fis-type)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR009057(Homeodomain-like)|interpro:IPR014317(Transcriptional activator, PspF)|interpro:IPR025943|interpro:IPR025944|interpro:IPR027417|rcsb pdb:2BJV|rcsb pdb:2BJW|rcsb pdb:2C96|rcsb pdb:2C98|rcsb pdb:2C99|rcsb pdb:2C9C|rcsb pdb:2VII|rcsb pdb:4QNM|rcsb pdb:4QNR|rcsb pdb:4QOS|rcsb pdb:5NSS|dip:DIP-10592N	-	-	-	figure legend:Supp FIg. 5A|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/04/21	2020/06/26	rogid:nkBWxxwZtM6XiHcyZqIy0sQLXS883333	rogid:VGlvabOQaaDDNH2dbOlcn/J098c83333	rigid:EivrzR8rPaiJ+DRi25kfNyafBAc	false	his tag:c-c|sufficient binding region:1-144	sufficient binding region:1-265	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0AFM6	uniprotkb:P37344	intact:EBI-1123459|uniprotkb:P23853|uniprotkb:P76040|uniprotkb:P77363|ensemblbacteria:AAC74386|ensemblbacteria:BAA14873	intact:EBI-1123431|uniprotkb:P76039|ensemblbacteria:AAC74385|ensemblbacteria:BAA14872	psi-mi:pspa_ecoli(display_long)|uniprotkb:pspA(gene name)|psi-mi:pspA(display_short)|uniprotkb:b1304(locus name)|uniprotkb:JW1297(locus name)	psi-mi:pspf_ecoli(display_long)|uniprotkb:pspF(gene name)|psi-mi:pspF(display_short)|uniprotkb:b1303(locus name)|uniprotkb:JW1296(locus name)|uniprotkb:ycjB(gene name synonym)|uniprotkb:Phage shock protein F(gene name synonym)	psi-mi:"MI:0028"(cosedimentation in solution)	Osadnik et al. (2015)	pubmed:26235546|imex:IM-28081	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25567879|imex:IM-28081-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_415820.1|refseq:WP_000511025.1|ensemblbacteria:AAC74386(transcript)|ensemblbacteria:b1304(gene)|ensemblbacteria:BAA14873(gene)|ensemblbacteria:BAA14873(transcript)|go:"GO:0005543"(phospholipid binding)|go:"GO:0005829"(cytosol)|go:"GO:0009271"(phage shock)|go:"GO:0009408"(response to heat)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043433"(negative regulation of DNA-binding transcription factor activity)|go:"GO:0060187"(cell pole)|interpro:IPR007157(PspA/IM30)|interpro:IPR014319(Phage shock protein A)|rcsb pdb:4WHE|dip:DIP-10587N	refseq:NP_415819.4|refseq:WP_001301108.1|ensemblbacteria:AAC74385(transcript)|ensemblbacteria:b1303(gene)|ensemblbacteria:BAA14872(gene)|ensemblbacteria:BAA14872(transcript)|go:"GO:0000160"(phosphorelay signal transduction system)|go:"GO:0003677"(DNA binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|interpro:IPR002078(RNA polymerase sigma factor 54, interaction)|interpro:IPR002197(Helix-turn-helix, Fis-type)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR009057(Homeodomain-like)|interpro:IPR014317(Transcriptional activator, PspF)|interpro:IPR025943|interpro:IPR025944|interpro:IPR027417|rcsb pdb:2BJV|rcsb pdb:2BJW|rcsb pdb:2C96|rcsb pdb:2C98|rcsb pdb:2C99|rcsb pdb:2C9C|rcsb pdb:2VII|rcsb pdb:4QNM|rcsb pdb:4QNR|rcsb pdb:4QOS|rcsb pdb:5NSS|dip:DIP-10592N	-	-	-	figure legend:FIg. 5A|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:1.0x10^-6(molar)	2020/04/21	2020/06/26	rogid:nkBWxxwZtM6XiHcyZqIy0sQLXS883333	rogid:VGlvabOQaaDDNH2dbOlcn/J098c83333	rigid:EivrzR8rPaiJ+DRi25kfNyafBAc	false	his tag:c-c|sufficient binding region:1-144|mutation with no effect:59-59|mutation decreasing interaction strength:37-37	sufficient binding region:1-265	6	6	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P37344	uniprotkb:P37344	intact:EBI-1123431|uniprotkb:P76039|ensemblbacteria:AAC74385|ensemblbacteria:BAA14872	intact:EBI-1123431|uniprotkb:P76039|ensemblbacteria:AAC74385|ensemblbacteria:BAA14872	psi-mi:pspf_ecoli(display_long)|uniprotkb:pspF(gene name)|psi-mi:pspF(display_short)|uniprotkb:b1303(locus name)|uniprotkb:JW1296(locus name)|uniprotkb:ycjB(gene name synonym)|uniprotkb:Phage shock protein F(gene name synonym)	psi-mi:pspf_ecoli(display_long)|uniprotkb:pspF(gene name)|psi-mi:pspF(display_short)|uniprotkb:b1303(locus name)|uniprotkb:JW1296(locus name)|uniprotkb:ycjB(gene name synonym)|uniprotkb:Phage shock protein F(gene name synonym)	psi-mi:"MI:0028"(cosedimentation in solution)	Osadnik et al. (2015)	pubmed:26235546|imex:IM-28081	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25568067|imex:IM-28081-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_415819.4|refseq:WP_001301108.1|ensemblbacteria:AAC74385(transcript)|ensemblbacteria:b1303(gene)|ensemblbacteria:BAA14872(gene)|ensemblbacteria:BAA14872(transcript)|go:"GO:0000160"(phosphorelay signal transduction system)|go:"GO:0003677"(DNA binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|interpro:IPR002078(RNA polymerase sigma factor 54, interaction)|interpro:IPR002197(Helix-turn-helix, Fis-type)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR009057(Homeodomain-like)|interpro:IPR014317(Transcriptional activator, PspF)|interpro:IPR025943|interpro:IPR025944|interpro:IPR027417|rcsb pdb:2BJV|rcsb pdb:2BJW|rcsb pdb:2C96|rcsb pdb:2C98|rcsb pdb:2C99|rcsb pdb:2C9C|rcsb pdb:2VII|rcsb pdb:4QNM|rcsb pdb:4QNR|rcsb pdb:4QOS|rcsb pdb:5NSS|dip:DIP-10592N	refseq:NP_415819.4|refseq:WP_001301108.1|ensemblbacteria:AAC74385(transcript)|ensemblbacteria:b1303(gene)|ensemblbacteria:BAA14872(gene)|ensemblbacteria:BAA14872(transcript)|go:"GO:0000160"(phosphorelay signal transduction system)|go:"GO:0003677"(DNA binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|interpro:IPR002078(RNA polymerase sigma factor 54, interaction)|interpro:IPR002197(Helix-turn-helix, Fis-type)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR009057(Homeodomain-like)|interpro:IPR014317(Transcriptional activator, PspF)|interpro:IPR025943|interpro:IPR025944|interpro:IPR027417|rcsb pdb:2BJV|rcsb pdb:2BJW|rcsb pdb:2C96|rcsb pdb:2C98|rcsb pdb:2C99|rcsb pdb:2C9C|rcsb pdb:2VII|rcsb pdb:4QNM|rcsb pdb:4QNR|rcsb pdb:4QOS|rcsb pdb:5NSS|dip:DIP-10592N	-	-	-	figure legend:Supp FIg. 5B, 5D|comment:Stable oligomerization of PspF1-265 was not promoted by addition of  ADP|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:1.0x10^-6(molar)	2020/04/21	2020/06/26	rogid:VGlvabOQaaDDNH2dbOlcn/J098c83333	rogid:VGlvabOQaaDDNH2dbOlcn/J098c83333	rigid:+AC933XEJk0Mev/tzo+wfbvLXmM	false	sufficient binding region:1-265	sufficient binding region:1-265	6	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15422"	intact:EBI-25568074	intact:EBI-1108845|chebi:"CHEBI:40938"|chebi:"CHEBI:10841"|chebi:"CHEBI:10789"|chebi:"CHEBI:2359"|chebi:"CHEBI:13236"|chebi:"CHEBI:22249"	-	psi-mi:atp(display_short)|psi-mi:"CHEBI:15422"(display_long)|intact:Adenosine 5'-triphosphate(synonym)|intact:Adenosine triphosphate(synonym)|intact:ADENOSINE-5'-TRIPHOSPHATE(synonym)|intact:Adephos(synonym)|intact:Adetol(synonym)|intact:Adynol(synonym)|intact:Atipi(synonym)|intact:ATP(synonym)|intact:Atriphos(synonym)|intact:Cardenosine(synonym)|intact:Fosfobion(synonym)|intact:Glucobasin(synonym)|intact:H4atp(synonym)|intact:Myotriphos(synonym)|intact:Triadenyl(synonym)|intact:Triphosphaden(synonym)|intact:"adenosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:pspaf_ecoli(display_short)|psi-mi:EBI-25568074(display_long)|intact:pspAF transcription regulation complex(complex recommended name)|intact:"6xpspA:6xpspF"(complex systematic name)	psi-mi:"MI:0880"(atpase assay)	Osadnik et al. (2015)	pubmed:26235546|imex:IM-28081	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0882"(atpase reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25568939|imex:IM-28081-9	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	go:"GO:0016887"(ATP hydrolysis activity)|intact:EBI-25567762(exp-evidence)|complex portal:CPX-5745(complex-primary)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0003700"(DNA-binding transcription factor activity)|pubmed:29906279(see-also)|pubmed:27297125(see-also)|go:"GO:0080135"(regulation of cellular response to stress)|go:"GO:0005667"(transcription regulator complex)|evidence ontology:"ECO:0000353"	go:"GO:0016887"(ATP hydrolysis activity)	-	curated-complex:"Transcription regulator, formation of which in the absence of membrane stress inhibits the ATPase activity of the enhancer binding and activator protein pspF. ATPase activity of pspF is required to induce an open complex formation of the DNA‐bound RNA polymerase at its target promoters. The pspAF complex retains only a basal ATPase activity. Membrane stresses, such as the mislocalisation of proteins to the inner membrane, appear to lead to the recruitment of pspA to the pspBC complex (CPX-5892), enabling at least a partial release of pspA from pspF. This allows transcription of the Psp membrane-stabilizing system through binding of pspF to the sigma factor rpoN (P24255)."|complex-properties:pspA binds to the pspF hexamer and inhibits pspF activity by direct interaction of the AAA+ transcription activation domain of pspF. pspA appears to bind to Trp-56 of pspF. This is part of the 50-62 aa loop which contains a group of three amino acids, called the YLW patch, that serve as a docking site for pspA and for ATP hydrolytic activity. The C-terminal domain of pspA appears to be crucial for the regulation of pspF in response to membrane stress stimuli.|complex-assembly:Heterododecamer	figure legend:Supp. Fig. 5E|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/04/21	2020/04/22	-	-	-	false	-	his tag:c-c|sufficient binding region:1-265|his tag:c-c|sufficient binding region:1-144	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)