#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q96QD9	uniprotkb:Q2HR75	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	intact:EBI-6884751|uniprotkb:O40938	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:"MI:0096"(pull down)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084016|imex:IM-27426-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	-	-	comment:"ORF57PmutGFP contains site-directed alterations of two proline residues within a PxxP poly-proline motif, situated in the previously identified minimal Aly-binding domain encompassing residues 181–215. ST-pulldown experiments and co-immunoprecipitation experiments were performed as described above using GST-ORF57Pmut and pORF57PmutGFP, respectively. In both cases the mutant ORF57 protein, which fails to bind Aly, also lacks the ability to interact with UIF (Figure 1A and 1B)."	figure legend:"fig. 1a(ii)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/10/09	2019/10/10	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rigid:/Hh8bE162GoFxWVo45nnyrvTzLE	false	flag tag:?-?	glutathione s tranferase tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q96QD9	uniprotkb:Q13838	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	intact:EBI-348622|uniprotkb:B0S8C0|uniprotkb:Q5STB8|uniprotkb:O43496|uniprotkb:Q0EFA1|uniprotkb:Q2L6F9|uniprotkb:Q53GL9|uniprotkb:Q5RJ64|uniprotkb:Q5RJ66|uniprotkb:Q5ST94|uniprotkb:Q5STB4|uniprotkb:Q5STB5|uniprotkb:Q5STB7|uniprotkb:Q5STU4|uniprotkb:Q5STU5|uniprotkb:Q5STU6|uniprotkb:Q5STU8|uniprotkb:Q71V76|ensembl:ENSP00000373000|ensembl:ENSP00000379475|ensembl:ENSP00000383151|ensembl:ENSP00000383152|ensembl:ENSP00000387994|ensembl:ENSP00000388880|ensembl:ENSP00000390999|ensembl:ENSP00000391463|ensembl:ENSP00000393712|ensembl:ENSP00000394160|ensembl:ENSP00000398775|ensembl:ENSP00000399030|ensembl:ENSP00000400326|ensembl:ENSP00000404695|ensembl:ENSP00000405560|ensembl:ENSP00000407419|ensembl:ENSP00000411136|ensembl:ENSP00000411853|ensembl:ENSP00000416269	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:dx39b_human(display_long)|uniprotkb:UAP56(gene name synonym)|uniprotkb:DDX39B(gene name)|psi-mi:DDX39B(display_short)|uniprotkb:DEAD box protein UAP56(gene name synonym)|uniprotkb:56 kDa U2AF65-associated protein(gene name synonym)|uniprotkb:ATP-dependent RNA helicase p47(gene name synonym)|uniprotkb:HLA-B-associated transcript 1 protein(gene name synonym)|uniprotkb:BAT1(gene name synonym)	psi-mi:"MI:0096"(pull down)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084483|imex:IM-27426-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	refseq:NP_004631.1|refseq:NP_542165.1|ensembl:ENSG00000198563(gene)|ensembl:ENSG00000215425(gene)|ensembl:ENSG00000225073(gene)|ensembl:ENSG00000225859(gene)|ensembl:ENSG00000229496(gene)|ensembl:ENSG00000230624(gene)|ensembl:ENSG00000235439(gene)|ensembl:ENST00000383508(transcript)|ensembl:ENST00000396172(transcript)|ensembl:ENST00000400295(transcript)|ensembl:ENST00000400296(transcript)|ensembl:ENST00000412106(transcript)|ensembl:ENST00000412330(transcript)|ensembl:ENST00000413678(transcript)|ensembl:ENST00000414440(transcript)|ensembl:ENST00000415689(transcript)|ensembl:ENST00000416863(transcript)|ensembl:ENST00000430784(transcript)|ensembl:ENST00000431360(transcript)|ensembl:ENST00000441425(transcript)|ensembl:ENST00000445218(transcript)|ensembl:ENST00000448296(transcript)|ensembl:ENST00000453138(transcript)|ensembl:ENST00000456476(transcript)|ensembl:ENST00000456666(transcript)|ensembl:ENST00000458640(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000346"(transcription export complex)|go:"GO:0001889"(liver development)|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005688"(U6 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0006405"(RNA export from nucleus)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0008186"(ATP-dependent activity, acting on RNA)|go:"GO:0008380"(RNA splicing)|go:"GO:0010501"(RNA secondary structure unwinding)|go:"GO:0016363"(nuclear matrix)|go:"GO:0016607"(nuclear speck)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0030621"(U4 snRNA binding)|go:"GO:0032786"(positive regulation of DNA-templated transcription, elongation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043008"(ATP-dependent protein binding)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045727"(positive regulation of translation)|go:"GO:0046784"(viral mRNA export from host cell nucleus)|go:"GO:0061051"(positive regulation of cell growth involved in cardiac muscle cell development)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1904707"(positive regulation of vascular associated smooth muscle cell proliferation)|go:"GO:2000002"(negative regulation of DNA damage checkpoint)|go:"GO:2000573"(positive regulation of DNA biosynthetic process)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR014014(RNA helicase, DEAD-box type, Q motif)|interpro:IPR027417|mint:Q13838|rcsb pdb:1T5I|rcsb pdb:1T6N|rcsb pdb:1XTI|rcsb pdb:1XTJ|rcsb pdb:1XTK|rcsb pdb:7APK|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|reactome:R-HSA-9013418	-	-	comment:"ORF57PmutGFP contains site-directed alterations of two proline residues within a PxxP poly-proline motif, situated in the previously identified minimal Aly-binding domain encompassing residues 181–215. ST-pulldown experiments and co-immunoprecipitation experiments were performed as described above using GST-ORF57Pmut and pORF57PmutGFP, respectively. In both cases the mutant ORF57 protein, which fails to bind Aly, also lacks the ability to interact with UIF (Figure 1A and 1B)."	figure legend:"fig. 1a(ii)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/10/09	2019/10/10	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rogid:Cl/rf1oq31fZbLHC2foUXu9MBOE9606	rigid:9X49oH3b/oQqfjtT3ur4Dg8Jbco	false	flag tag:?-?	glutathione s tranferase tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q2HR75	uniprotkb:Q96QD9	intact:EBI-6884751|uniprotkb:O40938	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:"MI:0096"(pull down)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084623|imex:IM-27426-25	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	-	-	-	figure legend:fig. 5a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/10/09	2019/10/10	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rigid:/Hh8bE162GoFxWVo45nnyrvTzLE	false	glutathione s tranferase tag:?-?	his tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q13838	uniprotkb:Q96QD9	intact:EBI-348622|uniprotkb:B0S8C0|uniprotkb:Q5STB8|uniprotkb:O43496|uniprotkb:Q0EFA1|uniprotkb:Q2L6F9|uniprotkb:Q53GL9|uniprotkb:Q5RJ64|uniprotkb:Q5RJ66|uniprotkb:Q5ST94|uniprotkb:Q5STB4|uniprotkb:Q5STB5|uniprotkb:Q5STB7|uniprotkb:Q5STU4|uniprotkb:Q5STU5|uniprotkb:Q5STU6|uniprotkb:Q5STU8|uniprotkb:Q71V76|ensembl:ENSP00000373000|ensembl:ENSP00000379475|ensembl:ENSP00000383151|ensembl:ENSP00000383152|ensembl:ENSP00000387994|ensembl:ENSP00000388880|ensembl:ENSP00000390999|ensembl:ENSP00000391463|ensembl:ENSP00000393712|ensembl:ENSP00000394160|ensembl:ENSP00000398775|ensembl:ENSP00000399030|ensembl:ENSP00000400326|ensembl:ENSP00000404695|ensembl:ENSP00000405560|ensembl:ENSP00000407419|ensembl:ENSP00000411136|ensembl:ENSP00000411853|ensembl:ENSP00000416269	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	psi-mi:dx39b_human(display_long)|uniprotkb:UAP56(gene name synonym)|uniprotkb:DDX39B(gene name)|psi-mi:DDX39B(display_short)|uniprotkb:DEAD box protein UAP56(gene name synonym)|uniprotkb:56 kDa U2AF65-associated protein(gene name synonym)|uniprotkb:ATP-dependent RNA helicase p47(gene name synonym)|uniprotkb:HLA-B-associated transcript 1 protein(gene name synonym)|uniprotkb:BAT1(gene name synonym)	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:"MI:0096"(pull down)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084712|imex:IM-27426-15	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0684"(ancillary)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0684"(ancillary)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_004631.1|refseq:NP_542165.1|ensembl:ENSG00000198563(gene)|ensembl:ENSG00000215425(gene)|ensembl:ENSG00000225073(gene)|ensembl:ENSG00000225859(gene)|ensembl:ENSG00000229496(gene)|ensembl:ENSG00000230624(gene)|ensembl:ENSG00000235439(gene)|ensembl:ENST00000383508(transcript)|ensembl:ENST00000396172(transcript)|ensembl:ENST00000400295(transcript)|ensembl:ENST00000400296(transcript)|ensembl:ENST00000412106(transcript)|ensembl:ENST00000412330(transcript)|ensembl:ENST00000413678(transcript)|ensembl:ENST00000414440(transcript)|ensembl:ENST00000415689(transcript)|ensembl:ENST00000416863(transcript)|ensembl:ENST00000430784(transcript)|ensembl:ENST00000431360(transcript)|ensembl:ENST00000441425(transcript)|ensembl:ENST00000445218(transcript)|ensembl:ENST00000448296(transcript)|ensembl:ENST00000453138(transcript)|ensembl:ENST00000456476(transcript)|ensembl:ENST00000456666(transcript)|ensembl:ENST00000458640(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000346"(transcription export complex)|go:"GO:0001889"(liver development)|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005688"(U6 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0006405"(RNA export from nucleus)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0008186"(ATP-dependent activity, acting on RNA)|go:"GO:0008380"(RNA splicing)|go:"GO:0010501"(RNA secondary structure unwinding)|go:"GO:0016363"(nuclear matrix)|go:"GO:0016607"(nuclear speck)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0030621"(U4 snRNA binding)|go:"GO:0032786"(positive regulation of DNA-templated transcription, elongation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043008"(ATP-dependent protein binding)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045727"(positive regulation of translation)|go:"GO:0046784"(viral mRNA export from host cell nucleus)|go:"GO:0061051"(positive regulation of cell growth involved in cardiac muscle cell development)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1904707"(positive regulation of vascular associated smooth muscle cell proliferation)|go:"GO:2000002"(negative regulation of DNA damage checkpoint)|go:"GO:2000573"(positive regulation of DNA biosynthetic process)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR014014(RNA helicase, DEAD-box type, Q motif)|interpro:IPR027417|mint:Q13838|rcsb pdb:1T5I|rcsb pdb:1T6N|rcsb pdb:1XTI|rcsb pdb:1XTJ|rcsb pdb:1XTK|rcsb pdb:7APK|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|reactome:R-HSA-9013418	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	-	-	-	figure legend:fig. 5b|comment:"No interaction with GST or GST-UAP56 was observed in the presence of ORF57-6xHis alone. In contrast, an interaction between GST-UAP56 and ORF57 was observed in the presence of purified UIF-6xHis protein (Figure 5B), suggesting that UIF can facilitate the formation of the ORF57-hTREX complex."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/10/09	2019/10/14	rogid:Cl/rf1oq31fZbLHC2foUXu9MBOE9606	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rigid:A4z/IxXRgz7zMUNuroVjB2WPfjI	false	glutathione s tranferase tag:?-?	his tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q13838	uniprotkb:Q2HR75	intact:EBI-348622|uniprotkb:B0S8C0|uniprotkb:Q5STB8|uniprotkb:O43496|uniprotkb:Q0EFA1|uniprotkb:Q2L6F9|uniprotkb:Q53GL9|uniprotkb:Q5RJ64|uniprotkb:Q5RJ66|uniprotkb:Q5ST94|uniprotkb:Q5STB4|uniprotkb:Q5STB5|uniprotkb:Q5STB7|uniprotkb:Q5STU4|uniprotkb:Q5STU5|uniprotkb:Q5STU6|uniprotkb:Q5STU8|uniprotkb:Q71V76|ensembl:ENSP00000373000|ensembl:ENSP00000379475|ensembl:ENSP00000383151|ensembl:ENSP00000383152|ensembl:ENSP00000387994|ensembl:ENSP00000388880|ensembl:ENSP00000390999|ensembl:ENSP00000391463|ensembl:ENSP00000393712|ensembl:ENSP00000394160|ensembl:ENSP00000398775|ensembl:ENSP00000399030|ensembl:ENSP00000400326|ensembl:ENSP00000404695|ensembl:ENSP00000405560|ensembl:ENSP00000407419|ensembl:ENSP00000411136|ensembl:ENSP00000411853|ensembl:ENSP00000416269	intact:EBI-6884751|uniprotkb:O40938	psi-mi:dx39b_human(display_long)|uniprotkb:UAP56(gene name synonym)|uniprotkb:DDX39B(gene name)|psi-mi:DDX39B(display_short)|uniprotkb:DEAD box protein UAP56(gene name synonym)|uniprotkb:56 kDa U2AF65-associated protein(gene name synonym)|uniprotkb:ATP-dependent RNA helicase p47(gene name synonym)|uniprotkb:HLA-B-associated transcript 1 protein(gene name synonym)|uniprotkb:BAT1(gene name synonym)	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:"MI:0096"(pull down)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084712|imex:IM-27426-15	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_004631.1|refseq:NP_542165.1|ensembl:ENSG00000198563(gene)|ensembl:ENSG00000215425(gene)|ensembl:ENSG00000225073(gene)|ensembl:ENSG00000225859(gene)|ensembl:ENSG00000229496(gene)|ensembl:ENSG00000230624(gene)|ensembl:ENSG00000235439(gene)|ensembl:ENST00000383508(transcript)|ensembl:ENST00000396172(transcript)|ensembl:ENST00000400295(transcript)|ensembl:ENST00000400296(transcript)|ensembl:ENST00000412106(transcript)|ensembl:ENST00000412330(transcript)|ensembl:ENST00000413678(transcript)|ensembl:ENST00000414440(transcript)|ensembl:ENST00000415689(transcript)|ensembl:ENST00000416863(transcript)|ensembl:ENST00000430784(transcript)|ensembl:ENST00000431360(transcript)|ensembl:ENST00000441425(transcript)|ensembl:ENST00000445218(transcript)|ensembl:ENST00000448296(transcript)|ensembl:ENST00000453138(transcript)|ensembl:ENST00000456476(transcript)|ensembl:ENST00000456666(transcript)|ensembl:ENST00000458640(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000346"(transcription export complex)|go:"GO:0001889"(liver development)|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005688"(U6 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0006405"(RNA export from nucleus)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0008186"(ATP-dependent activity, acting on RNA)|go:"GO:0008380"(RNA splicing)|go:"GO:0010501"(RNA secondary structure unwinding)|go:"GO:0016363"(nuclear matrix)|go:"GO:0016607"(nuclear speck)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0030621"(U4 snRNA binding)|go:"GO:0032786"(positive regulation of DNA-templated transcription, elongation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043008"(ATP-dependent protein binding)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045727"(positive regulation of translation)|go:"GO:0046784"(viral mRNA export from host cell nucleus)|go:"GO:0061051"(positive regulation of cell growth involved in cardiac muscle cell development)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1904707"(positive regulation of vascular associated smooth muscle cell proliferation)|go:"GO:2000002"(negative regulation of DNA damage checkpoint)|go:"GO:2000573"(positive regulation of DNA biosynthetic process)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR014014(RNA helicase, DEAD-box type, Q motif)|interpro:IPR027417|mint:Q13838|rcsb pdb:1T5I|rcsb pdb:1T6N|rcsb pdb:1XTI|rcsb pdb:1XTJ|rcsb pdb:1XTK|rcsb pdb:7APK|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|reactome:R-HSA-9013418	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	-	-	-	figure legend:fig. 5b|comment:"No interaction with GST or GST-UAP56 was observed in the presence of ORF57-6xHis alone. In contrast, an interaction between GST-UAP56 and ORF57 was observed in the presence of purified UIF-6xHis protein (Figure 5B), suggesting that UIF can facilitate the formation of the ORF57-hTREX complex."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/10/09	2019/10/14	rogid:Cl/rf1oq31fZbLHC2foUXu9MBOE9606	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rigid:A4z/IxXRgz7zMUNuroVjB2WPfjI	false	glutathione s tranferase tag:?-?	his tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q96QD9	uniprotkb:Q2HR75	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	intact:EBI-6884751|uniprotkb:O40938	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084503|imex:IM-27426-21	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	-	-	comment:"ORF57PmutGFP contains site-directed alterations of two proline residues within a PxxP poly-proline motif, situated in the previously identified minimal Aly-binding domain encompassing residues 181–215. GST-pulldown experiments and co-immunoprecipitation experiments were performed as described above using GST-ORF57Pmut and pORF57PmutGFP, respectively. In both cases the mutant ORF57 protein, which fails to bind Aly, also lacks the ability to interact with UIF (Figure 1A and 1B)."	figure legend:fig. 1b,|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2019/10/09	2019/10/10	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rigid:/Hh8bE162GoFxWVo45nnyrvTzLE	false	flag tag:?-?	green fluorescent protein tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q96QD9	uniprotkb:Q86V81	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	intact:EBI-347640|uniprotkb:O43672	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:thoc4_human(display_long)|uniprotkb:ALYREF(gene name)|psi-mi:ALYREF(display_short)|uniprotkb:ALY(gene name synonym)|uniprotkb:Ally of AML-1 and LEF-1(gene name synonym)|uniprotkb:Aly/REF export factor(gene name synonym)|uniprotkb:BEF(gene name synonym)|uniprotkb:THOC4(gene name synonym)|uniprotkb:Transcriptional coactivator Aly/REF(gene name synonym)|uniprotkb:bZIP-enhancing factor BEF(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084528|imex:IM-27426-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	refseq:NP_005773.3|mint:Q86V81|dip:DIP-32711N|go:"GO:0000018"(regulation of DNA recombination)|go:"GO:0000346"(transcription export complex)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0001649"(osteoblast differentiation)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006405"(RNA export from nucleus)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016020"(membrane)|go:"GO:0016607"(nuclear speck)|go:"GO:0031297"(replication fork processing)|go:"GO:0032786"(positive regulation of DNA-templated transcription, elongation)|go:"GO:0046784"(viral mRNA export from host cell nucleus)|go:"GO:0062153"(C5-methylcytidine-containing RNA binding)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR035979|rcsb pdb:3ULH|reactome:R-HSA-159227|reactome:R-HSA-159230|reactome:R-HSA-159231|reactome:R-HSA-159236|reactome:R-HSA-72163|reactome:R-HSA-72187|reactome:R-HSA-73856|interpro:IPR025715	-	-	comment:"ORF57PmutGFP contains site-directed alterations of two proline residues within a PxxP poly-proline motif, situated in the previously identified minimal Aly-binding domain encompassing residues 181–215. GST-pulldown experiments and co-immunoprecipitation experiments were performed as described above using GST-ORF57Pmut and pORF57PmutGFP, respectively. In both cases the mutant ORF57 protein, which fails to bind Aly, also lacks the ability to interact with UIF (Figure 1A and 1B)."	figure legend:fig. 1c|comment:"ORF57 was readily precipitated using the Flag-specific antibody in contrast to negative controls. Moreover, the observed interaction was still detected in the presence of RNase suggesting the interactions are not due to RNA bridging (Figure 1C)"|variable_condition:RNAse treatment inhibit interaction|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2019/10/09	2019/10/10	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rogid:beNqQmz9NZk8EdpXoQs0lIxOvTk9606	rigid:GnOBomI+BdJbFVy/NI2oxMCWgv4	false	flag tag:?-?	green fluorescent protein tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q96QD9	uniprotkb:Q2HR75	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	intact:EBI-6884751|uniprotkb:O40938	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084538|imex:IM-27426-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	-	-	comment:"ORF57PmutGFP contains site-directed alterations of two proline residues within a PxxP poly-proline motif, situated in the previously identified minimal Aly-binding domain encompassing residues 181–215. GST-pulldown experiments and co-immunoprecipitation experiments were performed as described above using GST-ORF57Pmut and pORF57PmutGFP, respectively. In both cases the mutant ORF57 protein, which fails to bind Aly, also lacks the ability to interact with UIF (Figure 1A and 1B)."	figure legend:fig. 1c|comment:"ORF57 was readily precipitated using the Flag-specific antibody in contrast to negative controls. Moreover, the observed interaction was still detected in the presence of RNase suggesting the interactions are not due to RNA bridging (Figure 1C)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2019/10/09	2019/10/10	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rigid:/Hh8bE162GoFxWVo45nnyrvTzLE	false	flag tag:?-?	green fluorescent protein tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q2HR75	uniprotkb:Q86V81	intact:EBI-6884751|uniprotkb:O40938	intact:EBI-347640|uniprotkb:O43672	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:thoc4_human(display_long)|uniprotkb:ALYREF(gene name)|psi-mi:ALYREF(display_short)|uniprotkb:ALY(gene name synonym)|uniprotkb:Ally of AML-1 and LEF-1(gene name synonym)|uniprotkb:Aly/REF export factor(gene name synonym)|uniprotkb:BEF(gene name synonym)|uniprotkb:THOC4(gene name synonym)|uniprotkb:Transcriptional coactivator Aly/REF(gene name synonym)|uniprotkb:bZIP-enhancing factor BEF(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084731|imex:IM-27426-17	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	refseq:NP_005773.3|mint:Q86V81|dip:DIP-32711N|go:"GO:0000018"(regulation of DNA recombination)|go:"GO:0000346"(transcription export complex)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0001649"(osteoblast differentiation)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006405"(RNA export from nucleus)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016020"(membrane)|go:"GO:0016607"(nuclear speck)|go:"GO:0031297"(replication fork processing)|go:"GO:0032786"(positive regulation of DNA-templated transcription, elongation)|go:"GO:0046784"(viral mRNA export from host cell nucleus)|go:"GO:0062153"(C5-methylcytidine-containing RNA binding)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR035979|rcsb pdb:3ULH|reactome:R-HSA-159227|reactome:R-HSA-159230|reactome:R-HSA-159231|reactome:R-HSA-159236|reactome:R-HSA-72163|reactome:R-HSA-72187|reactome:R-HSA-73856|interpro:IPR025715	-	comment:"ORF57PmutGFP contains site-directed alterations of two proline residues within a PxxP poly-proline motif, situated in the previously identified minimal Aly-binding domain encompassing residues 181–215. GST-pulldown experiments and co-immunoprecipitation experiments were performed as described above using GST-ORF57Pmut and pORF57PmutGFP, respectively. In both cases the mutant ORF57 protein, which fails to bind Aly, also lacks the ability to interact with UIF (Figure 1A and 1B)."	-	figure legend:"fig. 6b (i)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2019/10/09	2019/10/10	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rogid:beNqQmz9NZk8EdpXoQs0lIxOvTk9606	rigid:XjDePWkSMambYR2BgjOaw8gooo8	false	green fluorescent protein tag:?-?	myc tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q2HR75	uniprotkb:Q96QD9	intact:EBI-6884751|uniprotkb:O40938	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084731|imex:IM-27426-17	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	-	comment:"ORF57PmutGFP contains site-directed alterations of two proline residues within a PxxP poly-proline motif, situated in the previously identified minimal Aly-binding domain encompassing residues 181–215. GST-pulldown experiments and co-immunoprecipitation experiments were performed as described above using GST-ORF57Pmut and pORF57PmutGFP, respectively. In both cases the mutant ORF57 protein, which fails to bind Aly, also lacks the ability to interact with UIF (Figure 1A and 1B)."	-	figure legend:"fig. 6b (i)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2019/10/09	2019/10/10	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rigid:XjDePWkSMambYR2BgjOaw8gooo8	false	green fluorescent protein tag:?-?	flag tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q2HR75	uniprotkb:Q86V81	intact:EBI-6884751|uniprotkb:O40938	intact:EBI-347640|uniprotkb:O43672	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:thoc4_human(display_long)|uniprotkb:ALYREF(gene name)|psi-mi:ALYREF(display_short)|uniprotkb:ALY(gene name synonym)|uniprotkb:Ally of AML-1 and LEF-1(gene name synonym)|uniprotkb:Aly/REF export factor(gene name synonym)|uniprotkb:BEF(gene name synonym)|uniprotkb:THOC4(gene name synonym)|uniprotkb:Transcriptional coactivator Aly/REF(gene name synonym)|uniprotkb:bZIP-enhancing factor BEF(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084750|imex:IM-27426-19	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	refseq:NP_005773.3|mint:Q86V81|dip:DIP-32711N|go:"GO:0000018"(regulation of DNA recombination)|go:"GO:0000346"(transcription export complex)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0001649"(osteoblast differentiation)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006405"(RNA export from nucleus)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016020"(membrane)|go:"GO:0016607"(nuclear speck)|go:"GO:0031297"(replication fork processing)|go:"GO:0032786"(positive regulation of DNA-templated transcription, elongation)|go:"GO:0046784"(viral mRNA export from host cell nucleus)|go:"GO:0062153"(C5-methylcytidine-containing RNA binding)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR035979|rcsb pdb:3ULH|reactome:R-HSA-159227|reactome:R-HSA-159230|reactome:R-HSA-159231|reactome:R-HSA-159236|reactome:R-HSA-72163|reactome:R-HSA-72187|reactome:R-HSA-73856|interpro:IPR025715	-	comment:"ORF57PmutGFP contains site-directed alterations of two proline residues within a PxxP poly-proline motif, situated in the previously identified minimal Aly-binding domain encompassing residues 181–215. GST-pulldown experiments and co-immunoprecipitation experiments were performed as described above using GST-ORF57Pmut and pORF57PmutGFP, respectively. In both cases the mutant ORF57 protein, which fails to bind Aly, also lacks the ability to interact with UIF (Figure 1A and 1B)."	-	figure legend:"fig. 6b (ii)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2019/10/09	2019/10/10	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rogid:beNqQmz9NZk8EdpXoQs0lIxOvTk9606	rigid:XjDePWkSMambYR2BgjOaw8gooo8	false	green fluorescent protein tag:?-?	myc tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q2HR75	uniprotkb:Q96QD9	intact:EBI-6884751|uniprotkb:O40938	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084750|imex:IM-27426-19	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	-	comment:"ORF57PmutGFP contains site-directed alterations of two proline residues within a PxxP poly-proline motif, situated in the previously identified minimal Aly-binding domain encompassing residues 181–215. GST-pulldown experiments and co-immunoprecipitation experiments were performed as described above using GST-ORF57Pmut and pORF57PmutGFP, respectively. In both cases the mutant ORF57 protein, which fails to bind Aly, also lacks the ability to interact with UIF (Figure 1A and 1B)."	-	figure legend:"fig. 6b (ii)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2019/10/09	2019/10/10	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rigid:XjDePWkSMambYR2BgjOaw8gooo8	false	green fluorescent protein tag:?-?	flag tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q96QD9	uniprotkb:Q13838	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	intact:EBI-348622|uniprotkb:B0S8C0|uniprotkb:Q5STB8|uniprotkb:O43496|uniprotkb:Q0EFA1|uniprotkb:Q2L6F9|uniprotkb:Q53GL9|uniprotkb:Q5RJ64|uniprotkb:Q5RJ66|uniprotkb:Q5ST94|uniprotkb:Q5STB4|uniprotkb:Q5STB5|uniprotkb:Q5STB7|uniprotkb:Q5STU4|uniprotkb:Q5STU5|uniprotkb:Q5STU6|uniprotkb:Q5STU8|uniprotkb:Q71V76|ensembl:ENSP00000373000|ensembl:ENSP00000379475|ensembl:ENSP00000383151|ensembl:ENSP00000383152|ensembl:ENSP00000387994|ensembl:ENSP00000388880|ensembl:ENSP00000390999|ensembl:ENSP00000391463|ensembl:ENSP00000393712|ensembl:ENSP00000394160|ensembl:ENSP00000398775|ensembl:ENSP00000399030|ensembl:ENSP00000400326|ensembl:ENSP00000404695|ensembl:ENSP00000405560|ensembl:ENSP00000407419|ensembl:ENSP00000411136|ensembl:ENSP00000411853|ensembl:ENSP00000416269	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:dx39b_human(display_long)|uniprotkb:UAP56(gene name synonym)|uniprotkb:DDX39B(gene name)|psi-mi:DDX39B(display_short)|uniprotkb:DEAD box protein UAP56(gene name synonym)|uniprotkb:56 kDa U2AF65-associated protein(gene name synonym)|uniprotkb:ATP-dependent RNA helicase p47(gene name synonym)|uniprotkb:HLA-B-associated transcript 1 protein(gene name synonym)|uniprotkb:BAT1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084519|imex:IM-27426-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	refseq:NP_004631.1|refseq:NP_542165.1|ensembl:ENSG00000198563(gene)|ensembl:ENSG00000215425(gene)|ensembl:ENSG00000225073(gene)|ensembl:ENSG00000225859(gene)|ensembl:ENSG00000229496(gene)|ensembl:ENSG00000230624(gene)|ensembl:ENSG00000235439(gene)|ensembl:ENST00000383508(transcript)|ensembl:ENST00000396172(transcript)|ensembl:ENST00000400295(transcript)|ensembl:ENST00000400296(transcript)|ensembl:ENST00000412106(transcript)|ensembl:ENST00000412330(transcript)|ensembl:ENST00000413678(transcript)|ensembl:ENST00000414440(transcript)|ensembl:ENST00000415689(transcript)|ensembl:ENST00000416863(transcript)|ensembl:ENST00000430784(transcript)|ensembl:ENST00000431360(transcript)|ensembl:ENST00000441425(transcript)|ensembl:ENST00000445218(transcript)|ensembl:ENST00000448296(transcript)|ensembl:ENST00000453138(transcript)|ensembl:ENST00000456476(transcript)|ensembl:ENST00000456666(transcript)|ensembl:ENST00000458640(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000346"(transcription export complex)|go:"GO:0001889"(liver development)|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005688"(U6 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0006405"(RNA export from nucleus)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0008186"(ATP-dependent activity, acting on RNA)|go:"GO:0008380"(RNA splicing)|go:"GO:0010501"(RNA secondary structure unwinding)|go:"GO:0016363"(nuclear matrix)|go:"GO:0016607"(nuclear speck)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0030621"(U4 snRNA binding)|go:"GO:0032786"(positive regulation of DNA-templated transcription, elongation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043008"(ATP-dependent protein binding)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045727"(positive regulation of translation)|go:"GO:0046784"(viral mRNA export from host cell nucleus)|go:"GO:0061051"(positive regulation of cell growth involved in cardiac muscle cell development)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1904707"(positive regulation of vascular associated smooth muscle cell proliferation)|go:"GO:2000002"(negative regulation of DNA damage checkpoint)|go:"GO:2000573"(positive regulation of DNA biosynthetic process)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR014014(RNA helicase, DEAD-box type, Q motif)|interpro:IPR027417|mint:Q13838|rcsb pdb:1T5I|rcsb pdb:1T6N|rcsb pdb:1XTI|rcsb pdb:1XTJ|rcsb pdb:1XTK|rcsb pdb:7APK|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|reactome:R-HSA-9013418	-	-	-	figure legend:fig. 1b|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2019/10/09	2019/10/10	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rogid:Cl/rf1oq31fZbLHC2foUXu9MBOE9606	rigid:9X49oH3b/oQqfjtT3ur4Dg8Jbco	false	flag tag:?-?	myc tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q96QD9	uniprotkb:Q2HR75	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	intact:EBI-6884751|uniprotkb:O40938	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084573|imex:IM-27426-11	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	-	-	-	figure legend:fig. 2|comment:"In order to address potential overexpression artefacts of the above co-immunoprecipitation experiments and also determine whether ORF57 interacts with UIF during KSHV lytic replication, latently-infected BCBL-1 cells remained uninduced or reactivated using the phorbol ester, TPA. Lytic expression was confirmed by the detection of ORF57 using Western blot analysis in the reactivated samples (Figure 2). Uninduced and reactivated cell lysates were then incubated with no antibody control, ORF57- or UIF-specific antibodies. Reciprocal western blot analysis using the antibodies in reverse demonstrated that ORF57 interacts with UIF during KSHV lytic replication (Figure 2). Therefore, these data provide the first evidence of a viral protein associating with UIF."|variable_condition:TPA treatment|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-bcbl_1)|taxid:9606(Homo sapiens lymphoma cell line)	-	2019/10/09	2019/10/10	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rigid:/Hh8bE162GoFxWVo45nnyrvTzLE	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q96QD9	uniprotkb:Q2HR75	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	intact:EBI-6884751|uniprotkb:O40938	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084579|imex:IM-27426-23	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	-	-	-	figure legend:fig. 2|comment:"In order to address potential overexpression artefacts of the above co-immunoprecipitation experiments and also determine whether ORF57 interacts with UIF during KSHV lytic replication, latently-infected BCBL-1 cells remained uninduced or reactivated using the phorbol ester, TPA. Lytic expression was confirmed by the detection of ORF57 using Western blot analysis in the reactivated samples (Figure 2). Uninduced and reactivated cell lysates were then incubated with no antibody control, ORF57- or UIF-specific antibodies. Reciprocal western blot analysis using the antibodies in reverse demonstrated that ORF57 interacts with UIF during KSHV lytic replication (Figure 2). Therefore, these data provide the first evidence of a viral protein associating with UIF."|variable_condition:TPA treatment|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-bcbl_1)|taxid:9606(Homo sapiens lymphoma cell line)	-	2019/10/09	2019/10/10	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rigid:/Hh8bE162GoFxWVo45nnyrvTzLE	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q2HR75	uniprotkb:Q96QD9	intact:EBI-6884751|uniprotkb:O40938	intact:EBI-724553|uniprotkb:Q96SL9|uniprotkb:Q9BQI8|uniprotkb:A8MY74|uniprotkb:B2RCB2|uniprotkb:B7Z3R4|uniprotkb:B7Z7V1|uniprotkb:B7Z8I0|uniprotkb:B7ZAJ3|uniprotkb:C9J7P6|uniprotkb:C9JNG6|uniprotkb:C9JTH3|uniprotkb:C9JY50|ensembl:ENSP00000241502	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:uif_human(display_long)|uniprotkb:UIF(gene name synonym)|uniprotkb:FYTTD1(gene name)|psi-mi:FYTTD1(display_short)|uniprotkb:Forty-two-three domain-containing protein 1(gene name synonym)	psi-mi:"MI:0428"(imaging technique)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084607|imex:IM-27426-13	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	refseq:NP_001011537.2|refseq:NP_115664.2|ensembl:ENSG00000122068(gene)|ensembl:ENST00000241502(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0016607"(nuclear speck)|interpro:IPR009782(Protein of unknown function DUF1346)|mint:Q96QD9|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|dip:DIP-62119N	-	-	-	figure legend:fig. 3b, 3d|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2019/10/09	2019/10/10	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rogid:mMARxli9v/w8ggb5tjd/5hvSxnI9606	rigid:/Hh8bE162GoFxWVo45nnyrvTzLE	false	mcherry fluorescent protein tag:?-?	green fluorescent protein tag:?-?	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
uniprotkb:Q2HR75	uniprotkb:Q13769	intact:EBI-6884751|uniprotkb:O40938	intact:EBI-5280316|uniprotkb:O60839|uniprotkb:Q9UPZ5|ensembl:ENSP00000380969|ensembl:ENSP00000380970|ensembl:ENSP00000380971|ensembl:ENSP00000420306	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:thoc5_human(display_long)|uniprotkb:THOC5(gene name)|psi-mi:THOC5(display_short)|uniprotkb:C22orf19(gene name synonym)|uniprotkb:KIAA0983(gene name synonym)|uniprotkb:Functional spliceosome-associated protein 79(gene name synonym)|uniprotkb:NF2/meningioma region protein pK1.3(gene name synonym)|uniprotkb:Placental protein 39.2(gene name synonym)|uniprotkb:hTREX90(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084769|imex:IM-27426-27	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	refseq:NP_001002877.1|refseq:NP_001002878.1|refseq:NP_001002879.1|refseq:NP_003669.4|ensembl:ENSG00000100296(gene)|ensembl:ENST00000397871(transcript)|ensembl:ENST00000397872(transcript)|ensembl:ENST00000397873(transcript)|ensembl:ENST00000490103(transcript)|go:"GO:0000346"(transcription export complex)|go:"GO:0000347"(THO complex)|go:"GO:0000445"(THO complex part of transcription export complex)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0006397"(mRNA processing)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0008380"(RNA splicing)|go:"GO:0030224"(monocyte differentiation)|go:"GO:0032786"(positive regulation of DNA-templated transcription, elongation)|go:"GO:0046784"(viral mRNA export from host cell nucleus)|go:"GO:0060215"(primitive hemopoiesis)|go:"GO:2000002"(negative regulation of DNA damage checkpoint)|interpro:IPR019163|mint:Q13769|rcsb pdb:7APK|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856	-	comment:"ORF57PmutGFP contains site-directed alterations of two proline residues within a PxxP poly-proline motif, situated in the previously identified minimal Aly-binding domain encompassing residues 181–215. GST-pulldown experiments and co-immunoprecipitation experiments were performed as described above using GST-ORF57Pmut and pORF57PmutGFP, respectively. In both cases the mutant ORF57 protein, which fails to bind Aly, also lacks the ability to interact with UIF (Figure 1A and 1B)."	-	figure legend:"fig.7b (ii)"|comment:"results showed that expression of ORF57 in the wild type 293 cell line led to the precipitation of UAP56, FSAP79 and TAP suggesting that ORF57 expression leads to the formation of an export competent ribonucleoprotein particle (Figure 7B). Similar complex formation was observed in cell lines depleted singularly for Aly and UIF, where ORF57 can precipitate UAP56, FSAP79 and TAP (Figure 7B). In contrast, depletion of Aly and UIF in combination significantly reduced the interaction between ORF57 and the hTREX components and the nuclear export factor TAP. Importantly, these data demonstrate that either Aly or UIF are required for the formation of an ORF57-mediated nuclear export competent ribonucleoprotein particle."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2019/10/09	2019/10/14	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rogid:JoeUa2WhtV/dR2HSjUEBAHDZZtw9606	rigid:NpAUdjoM0EJbComI8TpZhjw+FLk	false	green fluorescent protein tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q2HR75	uniprotkb:Q13838	intact:EBI-6884751|uniprotkb:O40938	intact:EBI-348622|uniprotkb:B0S8C0|uniprotkb:Q5STB8|uniprotkb:O43496|uniprotkb:Q0EFA1|uniprotkb:Q2L6F9|uniprotkb:Q53GL9|uniprotkb:Q5RJ64|uniprotkb:Q5RJ66|uniprotkb:Q5ST94|uniprotkb:Q5STB4|uniprotkb:Q5STB5|uniprotkb:Q5STB7|uniprotkb:Q5STU4|uniprotkb:Q5STU5|uniprotkb:Q5STU6|uniprotkb:Q5STU8|uniprotkb:Q71V76|ensembl:ENSP00000373000|ensembl:ENSP00000379475|ensembl:ENSP00000383151|ensembl:ENSP00000383152|ensembl:ENSP00000387994|ensembl:ENSP00000388880|ensembl:ENSP00000390999|ensembl:ENSP00000391463|ensembl:ENSP00000393712|ensembl:ENSP00000394160|ensembl:ENSP00000398775|ensembl:ENSP00000399030|ensembl:ENSP00000400326|ensembl:ENSP00000404695|ensembl:ENSP00000405560|ensembl:ENSP00000407419|ensembl:ENSP00000411136|ensembl:ENSP00000411853|ensembl:ENSP00000416269	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:dx39b_human(display_long)|uniprotkb:UAP56(gene name synonym)|uniprotkb:DDX39B(gene name)|psi-mi:DDX39B(display_short)|uniprotkb:DEAD box protein UAP56(gene name synonym)|uniprotkb:56 kDa U2AF65-associated protein(gene name synonym)|uniprotkb:ATP-dependent RNA helicase p47(gene name synonym)|uniprotkb:HLA-B-associated transcript 1 protein(gene name synonym)|uniprotkb:BAT1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084769|imex:IM-27426-27	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	refseq:NP_004631.1|refseq:NP_542165.1|ensembl:ENSG00000198563(gene)|ensembl:ENSG00000215425(gene)|ensembl:ENSG00000225073(gene)|ensembl:ENSG00000225859(gene)|ensembl:ENSG00000229496(gene)|ensembl:ENSG00000230624(gene)|ensembl:ENSG00000235439(gene)|ensembl:ENST00000383508(transcript)|ensembl:ENST00000396172(transcript)|ensembl:ENST00000400295(transcript)|ensembl:ENST00000400296(transcript)|ensembl:ENST00000412106(transcript)|ensembl:ENST00000412330(transcript)|ensembl:ENST00000413678(transcript)|ensembl:ENST00000414440(transcript)|ensembl:ENST00000415689(transcript)|ensembl:ENST00000416863(transcript)|ensembl:ENST00000430784(transcript)|ensembl:ENST00000431360(transcript)|ensembl:ENST00000441425(transcript)|ensembl:ENST00000445218(transcript)|ensembl:ENST00000448296(transcript)|ensembl:ENST00000453138(transcript)|ensembl:ENST00000456476(transcript)|ensembl:ENST00000456666(transcript)|ensembl:ENST00000458640(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000346"(transcription export complex)|go:"GO:0001889"(liver development)|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005688"(U6 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0006405"(RNA export from nucleus)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0008186"(ATP-dependent activity, acting on RNA)|go:"GO:0008380"(RNA splicing)|go:"GO:0010501"(RNA secondary structure unwinding)|go:"GO:0016363"(nuclear matrix)|go:"GO:0016607"(nuclear speck)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0030621"(U4 snRNA binding)|go:"GO:0032786"(positive regulation of DNA-templated transcription, elongation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043008"(ATP-dependent protein binding)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045727"(positive regulation of translation)|go:"GO:0046784"(viral mRNA export from host cell nucleus)|go:"GO:0061051"(positive regulation of cell growth involved in cardiac muscle cell development)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1904707"(positive regulation of vascular associated smooth muscle cell proliferation)|go:"GO:2000002"(negative regulation of DNA damage checkpoint)|go:"GO:2000573"(positive regulation of DNA biosynthetic process)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR014014(RNA helicase, DEAD-box type, Q motif)|interpro:IPR027417|mint:Q13838|rcsb pdb:1T5I|rcsb pdb:1T6N|rcsb pdb:1XTI|rcsb pdb:1XTJ|rcsb pdb:1XTK|rcsb pdb:7APK|reactome:R-HSA-159236|reactome:R-HSA-72187|reactome:R-HSA-73856|reactome:R-HSA-9013418	-	comment:"ORF57PmutGFP contains site-directed alterations of two proline residues within a PxxP poly-proline motif, situated in the previously identified minimal Aly-binding domain encompassing residues 181–215. GST-pulldown experiments and co-immunoprecipitation experiments were performed as described above using GST-ORF57Pmut and pORF57PmutGFP, respectively. In both cases the mutant ORF57 protein, which fails to bind Aly, also lacks the ability to interact with UIF (Figure 1A and 1B)."	-	figure legend:"fig.7b (ii)"|comment:"results showed that expression of ORF57 in the wild type 293 cell line led to the precipitation of UAP56, FSAP79 and TAP suggesting that ORF57 expression leads to the formation of an export competent ribonucleoprotein particle (Figure 7B). Similar complex formation was observed in cell lines depleted singularly for Aly and UIF, where ORF57 can precipitate UAP56, FSAP79 and TAP (Figure 7B). In contrast, depletion of Aly and UIF in combination significantly reduced the interaction between ORF57 and the hTREX components and the nuclear export factor TAP. Importantly, these data demonstrate that either Aly or UIF are required for the formation of an ORF57-mediated nuclear export competent ribonucleoprotein particle."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2019/10/09	2019/10/14	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rogid:Cl/rf1oq31fZbLHC2foUXu9MBOE9606	rigid:NpAUdjoM0EJbComI8TpZhjw+FLk	false	green fluorescent protein tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q2HR75	uniprotkb:Q9UBU9	intact:EBI-6884751|uniprotkb:O40938	intact:EBI-398874|uniprotkb:Q99799|uniprotkb:Q9UQL2|uniprotkb:B4E269|ensembl:ENSP00000436679|ensembl:ENSP00000294172	psi-mi:icp27_hhv8p(display_long)|uniprotkb:EB2 protein homolog(gene name synonym)|uniprotkb:ORF57(orf name)	psi-mi:nxf1_human(display_long)|uniprotkb:NXF1(gene name)|psi-mi:NXF1(display_short)|uniprotkb:TAP(gene name synonym)|uniprotkb:Tip-associating protein(gene name synonym)|uniprotkb:Tip-associated protein(gene name synonym)|uniprotkb:mRNA export factor TAP(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Jackson et al. (2011)	pubmed:21814512|imex:IM-27426	taxid:868565(hhv8p)|taxid:868565(Human herpesvirus 8)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22084769|imex:IM-27426-27	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0003723"(RNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0042025"(host cell nucleus)|go:"GO:0046872"(metal ion binding)|rcsb pdb:5ZB1|rcsb pdb:5ZB3|interpro:IPR008648(Herpesvirus transcriptional regulator)|refseq:YP_001129410.1|mint:Q2HR75	refseq:NP_001074960.1|refseq:NP_006353.2|ensembl:ENSP00000294172|ensembl:ENST00000531709|ensembl:ENSP00000453885|ensembl:ENSP00000436679|dip:DIP-31789N|ensembl:ENST00000294172(transcript)|ensembl:ENST00000532297(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005643"(nuclear pore)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0010494"(cytoplasmic stress granule)|go:"GO:0015031"(protein transport)|go:"GO:0016607"(nuclear speck)|go:"GO:0016973"("poly(A)+ mRNA export from nucleus")|go:"GO:0042405"(nuclear inclusion body)|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR002075(Nuclear transport factor 2)|interpro:IPR005637(TAP, C-terminal)|interpro:IPR009060(UBA-like)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR015245(Tap, RNA-binding)|interpro:IPR018222|interpro:IPR030217|interpro:IPR032675|interpro:IPR032710|interpro:IPR035979|mint:Q9UBU9|rcsb pdb:1FO1|rcsb pdb:1FT8|rcsb pdb:1GO5|rcsb pdb:1JKG|rcsb pdb:1JN5|rcsb pdb:1KOH|rcsb pdb:1KOO|rcsb pdb:1OAI|rcsb pdb:2Z5K|rcsb pdb:2Z5M|rcsb pdb:3RW6|rcsb pdb:3RW7|rcsb pdb:4WYK|rcsb pdb:6E5U|reactome:R-HSA-159227|reactome:R-HSA-159230|reactome:R-HSA-159231|reactome:R-HSA-159236|ensembl:ENSG00000162231(gene)	-	comment:"ORF57PmutGFP contains site-directed alterations of two proline residues within a PxxP poly-proline motif, situated in the previously identified minimal Aly-binding domain encompassing residues 181–215. GST-pulldown experiments and co-immunoprecipitation experiments were performed as described above using GST-ORF57Pmut and pORF57PmutGFP, respectively. In both cases the mutant ORF57 protein, which fails to bind Aly, also lacks the ability to interact with UIF (Figure 1A and 1B)."	-	figure legend:"fig.7b (ii)"|comment:"results showed that expression of ORF57 in the wild type 293 cell line led to the precipitation of UAP56, FSAP79 and TAP suggesting that ORF57 expression leads to the formation of an export competent ribonucleoprotein particle (Figure 7B). Similar complex formation was observed in cell lines depleted singularly for Aly and UIF, where ORF57 can precipitate UAP56, FSAP79 and TAP (Figure 7B). In contrast, depletion of Aly and UIF in combination significantly reduced the interaction between ORF57 and the hTREX components and the nuclear export factor TAP. Importantly, these data demonstrate that either Aly or UIF are required for the formation of an ORF57-mediated nuclear export competent ribonucleoprotein particle."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2019/10/09	2019/10/14	rogid:uz5jAyUcPT0g+MBLU1hpzevlfJ8868565	rogid:a983QBMgsm07FV/IcYsO37Sk0hU9606	rigid:NpAUdjoM0EJbComI8TpZhjw+FLk	false	green fluorescent protein tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)