#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q8X482	uniprotkb:Q60598	intact:EBI-22229752|ensemblbacteria:AAG56991	intact:EBI-397955|intact:EBI-4281621|uniprotkb:Q3UGC2|ensembl:ENSMUSP00000099368	psi-mi:espfu_eco57(display_long)|uniprotkb:EspF-like protein encoded on prophage U(gene name synonym)|uniprotkb:Tir-cytoskeleton coupling protein TccP(gene name synonym)|uniprotkb:"espF(U)"(gene name)|psi-mi:"espF(U)"(display_short)|uniprotkb:tccP(gene name synonym)|uniprotkb:Z3072(locus name)	psi-mi:src8_mouse(display_long)|uniprotkb:Cttn(gene name)|psi-mi:Cttn(display_short)|uniprotkb:Ems1(gene name synonym)	psi-mi:"MI:0049"(filter binding)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-22267818|imex:IM-27552-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG56991(transcript)|ensemblbacteria:Z3072(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0030430"(host cell cytoplasm)|mint:Q8X482|interpro:IPR006891(EspF protein)	refseq:NP_031829.2|ensembl:ENSMUSG00000031078(gene)|ensembl:ENSMUST00000103079(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030054"(cell junction)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051015"(actin filament binding)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|interpro:IPR036028|mint:Q60598|rcsb pdb:2F9X|rcsb pdb:3ULR|rcsb pdb:5NV1|rcsb pdb:5NVJ|rcsb pdb:5NXJ|reactome:R-MMU-8856828|dip:DIP-31562N	-	-	-	figure legend:fig. 4a left|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/12/02	2019/12/05	rogid:5ta4O6gj+U5E7doG3zmjLy36nJ483334	rogid:4hLyMrquWdlJnIX2pHuXqYtQgkE10090	rigid:9yuum9lt9eFKpJdH0OAI3CyXGpI	false	his tag:c-c|myc tag:?-?	glutathione s tranferase tag:?-?|binding-associated region:488-546	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q60598	uniprotkb:Q7DB77	intact:EBI-397955|intact:EBI-4281621|uniprotkb:Q3UGC2|ensembl:ENSMUSP00000099368	intact:EBI-6480811|ensemblbacteria:AAG58825|ensemblbacteria:BAB37984|uniprotkb:Q7A9Q1|uniprotkb:Q9R396	psi-mi:src8_mouse(display_long)|uniprotkb:Cttn(gene name)|psi-mi:Cttn(display_short)|uniprotkb:Ems1(gene name synonym)	psi-mi:tir_eco57(display_long)|uniprotkb:tir(gene name)|psi-mi:tir(display_short)|uniprotkb:espE(gene name synonym)|uniprotkb:Secreted effector protein Tir(gene name synonym)|uniprotkb:Z5112(locus name)|uniprotkb:ECs4561(locus name)	psi-mi:"MI:0049"(filter binding)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-22274828|imex:IM-27552-12	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_031829.2|ensembl:ENSMUSG00000031078(gene)|ensembl:ENSMUST00000103079(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030054"(cell junction)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051015"(actin filament binding)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|interpro:IPR036028|mint:Q60598|rcsb pdb:2F9X|rcsb pdb:3ULR|rcsb pdb:5NV1|rcsb pdb:5NVJ|rcsb pdb:5NXJ|reactome:R-MMU-8856828|dip:DIP-31562N	mint:Q7DB77|ensemblbacteria:AAG58825(transcript)|ensemblbacteria:BAB37984(transcript)|ensemblbacteria:ECs_4561(gene)|ensemblbacteria:Z5112(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0016021"(integral component of membrane)|go:"GO:0020002"(host cell plasma membrane)|rcsb pdb:2ZQK|rcsb pdb:2ZWK|refseq:WP_001301454.1|refseq:NP_312588.1|interpro:IPR022638|interpro:IPR022639|interpro:IPR003536(Translocated intimin receptor)|interpro:IPR022633|interpro:IPR037003	-	-	-	figure legend:fig. 5a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/12/02	2019/12/09	rogid:4hLyMrquWdlJnIX2pHuXqYtQgkE10090	rogid:w091zQ2yloHiTr07621yQtr7n0w83334	rigid:hy21jRD8pgXiayiN2bTNc/HYd8g	false	ha tag:?-?	his tag:?-?|biological feature:1-172	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q7DB77	uniprotkb:Q60598	intact:EBI-6480811|ensemblbacteria:AAG58825|ensemblbacteria:BAB37984|uniprotkb:Q7A9Q1|uniprotkb:Q9R396	intact:EBI-397955|intact:EBI-4281621|uniprotkb:Q3UGC2|ensembl:ENSMUSP00000099368	psi-mi:tir_eco57(display_long)|uniprotkb:tir(gene name)|psi-mi:tir(display_short)|uniprotkb:espE(gene name synonym)|uniprotkb:Secreted effector protein Tir(gene name synonym)|uniprotkb:Z5112(locus name)|uniprotkb:ECs4561(locus name)	psi-mi:src8_mouse(display_long)|uniprotkb:Cttn(gene name)|psi-mi:Cttn(display_short)|uniprotkb:Ems1(gene name synonym)	psi-mi:"MI:0049"(filter binding)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-22274960|imex:IM-27552-16	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	mint:Q7DB77|ensemblbacteria:AAG58825(transcript)|ensemblbacteria:BAB37984(transcript)|ensemblbacteria:ECs_4561(gene)|ensemblbacteria:Z5112(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0016021"(integral component of membrane)|go:"GO:0020002"(host cell plasma membrane)|rcsb pdb:2ZQK|rcsb pdb:2ZWK|refseq:WP_001301454.1|refseq:NP_312588.1|interpro:IPR022638|interpro:IPR022639|interpro:IPR003536(Translocated intimin receptor)|interpro:IPR022633|interpro:IPR037003	refseq:NP_031829.2|ensembl:ENSMUSG00000031078(gene)|ensembl:ENSMUST00000103079(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030054"(cell junction)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051015"(actin filament binding)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|interpro:IPR036028|mint:Q60598|rcsb pdb:2F9X|rcsb pdb:3ULR|rcsb pdb:5NV1|rcsb pdb:5NVJ|rcsb pdb:5NXJ|reactome:R-MMU-8856828|dip:DIP-31562N	-	-	-	figure legend:fig. 5c|comment:"we performed a gel overlay assay using several GST‐tagged cortactin constructs immobilized onto PVDF membranes. His‐Tir‐Nt was added as the probe and was found to bind weakly to cortactin wild type or the S/D form (Fig. 5C). Compared with that, the binding of His‐Tir‐Nt to Cort Y/D seemed to be only slightly improved (Fig. 5C, Y/D). Then, we decided to phosphorylate the GST‐Cort wild type in vitro using recombinant c‐Src. As shown in Fig. 5C (wild‐pY), His‐Tir‐Nt reacted strongly with this construct, confirming that tyrosine phosphorylation of cortactin is required to bind the N‐terminus of Tir from EHEC."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/12/02	2019/12/09	rogid:w091zQ2yloHiTr07621yQtr7n0w83334	rogid:4hLyMrquWdlJnIX2pHuXqYtQgkE10090	rigid:hy21jRD8pgXiayiN2bTNc/HYd8g	false	his tag:c-c|binding-associated region:1-172	glutathione s tranferase tag:?-?|binding-associated region:488-546|mutation increasing interaction:421-421,466-466|mutation with no effect:405-405,418-418	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q8X482	uniprotkb:Q60598	intact:EBI-22229752|ensemblbacteria:AAG56991	intact:EBI-397955|intact:EBI-4281621|uniprotkb:Q3UGC2|ensembl:ENSMUSP00000099368	psi-mi:espfu_eco57(display_long)|uniprotkb:EspF-like protein encoded on prophage U(gene name synonym)|uniprotkb:Tir-cytoskeleton coupling protein TccP(gene name synonym)|uniprotkb:"espF(U)"(gene name)|psi-mi:"espF(U)"(display_short)|uniprotkb:tccP(gene name synonym)|uniprotkb:Z3072(locus name)	psi-mi:src8_mouse(display_long)|uniprotkb:Cttn(gene name)|psi-mi:Cttn(display_short)|uniprotkb:Ems1(gene name synonym)	psi-mi:"MI:0096"(pull down)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-22272826|imex:IM-27552-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG56991(transcript)|ensemblbacteria:Z3072(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0030430"(host cell cytoplasm)|mint:Q8X482|interpro:IPR006891(EspF protein)	refseq:NP_031829.2|ensembl:ENSMUSG00000031078(gene)|ensembl:ENSMUST00000103079(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030054"(cell junction)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051015"(actin filament binding)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|interpro:IPR036028|mint:Q60598|rcsb pdb:2F9X|rcsb pdb:3ULR|rcsb pdb:5NV1|rcsb pdb:5NVJ|rcsb pdb:5NXJ|reactome:R-MMU-8856828|dip:DIP-31562N	-	-	-	figure legend:fig. 4a centre|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/12/02	2019/12/05	rogid:5ta4O6gj+U5E7doG3zmjLy36nJ483334	rogid:4hLyMrquWdlJnIX2pHuXqYtQgkE10090	rigid:9yuum9lt9eFKpJdH0OAI3CyXGpI	false	his tag:c-c|myc tag:?-?	glutathione s tranferase tag:?-?|binding-associated region:488-546|mutation increasing interaction:421-421,466-466|mutation with no effect:405-405,418-418	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q14247	uniprotkb:Q8X482	intact:EBI-351886|intact:EBI-3959948|ensembl:ENSP00000301843|ensembl:ENSP00000500756|uniprotkb:Q8N707|uniprotkb:Q96H99	intact:EBI-22229752|ensemblbacteria:AAG56991	psi-mi:src8_human(display_long)|uniprotkb:CTTN(gene name)|psi-mi:CTTN(display_short)|uniprotkb:EMS1(gene name synonym)|uniprotkb:Amplaxin(gene name synonym)|uniprotkb:Oncogene EMS1(gene name synonym)	psi-mi:espfu_eco57(display_long)|uniprotkb:EspF-like protein encoded on prophage U(gene name synonym)|uniprotkb:Tir-cytoskeleton coupling protein TccP(gene name synonym)|uniprotkb:"espF(U)"(gene name)|psi-mi:"espF(U)"(display_short)|uniprotkb:tccP(gene name synonym)|uniprotkb:Z3072(locus name)	psi-mi:"MI:0416"(fluorescence microscopy)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-22272907|imex:IM-27552-6	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_005222.2|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045296"(cadherin binding)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0051015"(actin filament binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|interpro:IPR036028|mint:Q14247|rcsb pdb:1X69|rcsb pdb:2D1X|reactome:R-HSA-5627123|reactome:R-HSA-8856828|ensembl:ENSG00000085733(gene)|ensembl:ENSG00000288401(gene)|ensembl:ENST00000301843(transcript)|ensembl:ENST00000671805(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|refseq:NP_001171669.1|refseq:NP_612632.1|dip:DIP-33190N	ensemblbacteria:AAG56991(transcript)|ensemblbacteria:Z3072(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0030430"(host cell cytoplasm)|mint:Q8X482|interpro:IPR006891(EspF protein)	go:"GO:0042995"(cell projection)	-	-	figure legend:fig. 4c|comment:"Infection of HeLa cells with DMEM‐activated EHEC expressing FLAG‐tagged EspFu (as in Fig. 2C and D) resulted in a perfect colocalization of EspFu‐FLAG and tyrosine‐phosphorylated cortactin, which was visible 45 min after infection (Fig. 4C). As the infection proceeded (120 min), cortactin was dephosphorylated and only EspFu‐FLAG was detected near the bacteria."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-hela)|taxid:9606(Homo sapiens epitheloid cervix carcinoma cells)	-	2019/12/02	2019/12/05	rogid:uzhAnL5Fu9Mgg9/LWeNiaYIwNVU9606	rogid:5ta4O6gj+U5E7doG3zmjLy36nJ483334	rigid:AffvIfSUbfE+9f/+4IHLjr9WDL0	false	-	flag tag:?-?	-	-	psi-mi:"MI:0422"(immunostaining)	psi-mi:"MI:0707"(anti tag immunostaining)
uniprotkb:Q14247	uniprotkb:Q7DB77	intact:EBI-351886|intact:EBI-3959948|ensembl:ENSP00000301843|ensembl:ENSP00000500756|uniprotkb:Q8N707|uniprotkb:Q96H99	intact:EBI-6480811|ensemblbacteria:AAG58825|ensemblbacteria:BAB37984|uniprotkb:Q7A9Q1|uniprotkb:Q9R396	psi-mi:src8_human(display_long)|uniprotkb:CTTN(gene name)|psi-mi:CTTN(display_short)|uniprotkb:EMS1(gene name synonym)|uniprotkb:Amplaxin(gene name synonym)|uniprotkb:Oncogene EMS1(gene name synonym)	psi-mi:tir_eco57(display_long)|uniprotkb:tir(gene name)|psi-mi:tir(display_short)|uniprotkb:espE(gene name synonym)|uniprotkb:Secreted effector protein Tir(gene name synonym)|uniprotkb:Z5112(locus name)|uniprotkb:ECs4561(locus name)	psi-mi:"MI:0416"(fluorescence microscopy)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-22274999|imex:IM-27552-20	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_005222.2|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045296"(cadherin binding)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0051015"(actin filament binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|interpro:IPR036028|mint:Q14247|rcsb pdb:1X69|rcsb pdb:2D1X|reactome:R-HSA-5627123|reactome:R-HSA-8856828|ensembl:ENSG00000085733(gene)|ensembl:ENSG00000288401(gene)|ensembl:ENST00000301843(transcript)|ensembl:ENST00000671805(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|refseq:NP_001171669.1|refseq:NP_612632.1|dip:DIP-33190N	mint:Q7DB77|ensemblbacteria:AAG58825(transcript)|ensemblbacteria:BAB37984(transcript)|ensemblbacteria:ECs_4561(gene)|ensemblbacteria:Z5112(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0016021"(integral component of membrane)|go:"GO:0020002"(host cell plasma membrane)|rcsb pdb:2ZQK|rcsb pdb:2ZWK|refseq:WP_001301454.1|refseq:NP_312588.1|interpro:IPR022638|interpro:IPR022639|interpro:IPR003536(Translocated intimin receptor)|interpro:IPR022633|interpro:IPR037003	go:"GO:0042995"(cell projection)	-	-	figure legend:fig. 5e|comment:"Furthermore, using immunofluorescence, Tir and tyrosine‐phosphorylated cortactin were found to colocalize beneath the attached bacteria in HeLa cells infected with DMEM‐activated EHEC for 45 min (Fig. 5E). Accordingly, due to the cortactin dephosphorylation, only Tir was detected in HeLa cells infected with EHEC for 120 min. Infection of HeLa cells with DMEM‐activated EHEC Δtir resulted in a complete failure to induce tyrosine phosphorylation of cortactin, as well as actin assembly (Fig. 5E, Δtir)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-hela)|taxid:9606(Homo sapiens epitheloid cervix carcinoma cells)	-	2019/12/02	2019/12/09	rogid:uzhAnL5Fu9Mgg9/LWeNiaYIwNVU9606	rogid:w091zQ2yloHiTr07621yQtr7n0w83334	rigid:f2eYnqQ2M783YtQMAV8mrYPfjUY	false	-	myc tag:?-?	-	-	psi-mi:"MI:0422"(immunostaining)	psi-mi:"MI:0422"(immunostaining)
uniprotkb:Q60598	uniprotkb:O08816	intact:EBI-397955|intact:EBI-4281621|uniprotkb:Q3UGC2|ensembl:ENSMUSP00000099368	intact:EBI-6142604	psi-mi:src8_mouse(display_long)|uniprotkb:Cttn(gene name)|psi-mi:Cttn(display_short)|uniprotkb:Ems1(gene name synonym)	psi-mi:wasl_rat(display_long)|uniprotkb:Wasl(gene name)|psi-mi:Wasl(display_short)	psi-mi:"MI:0018"(two hybrid)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22274713|imex:IM-27552-26	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_031829.2|ensembl:ENSMUSG00000031078(gene)|ensembl:ENSMUST00000103079(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030054"(cell junction)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051015"(actin filament binding)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|interpro:IPR036028|mint:Q60598|rcsb pdb:2F9X|rcsb pdb:3ULR|rcsb pdb:5NV1|rcsb pdb:5NVJ|rcsb pdb:5NXJ|reactome:R-MMU-8856828|dip:DIP-31562N	mint:O08816|rgd:735144|dip:DIP-17016N|go:"GO:0003779"(actin binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0008092"(cytoskeletal protein binding)|go:"GO:0009617"(response to bacterium)|go:"GO:0010324"(membrane invagination)|go:"GO:0016050"(vesicle organization)|go:"GO:0030027"(lamellipodium)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030048"(actin filament-based movement)|go:"GO:0030050"(vesicle transport along actin filament)|go:"GO:0030478"(actin cap)|go:"GO:0031252"(cell leading edge)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032880"(regulation of protein localization)|go:"GO:0034629"|go:"GO:0042802"(identical protein binding)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051301"(cell division)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0051653"(spindle localization)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0060997"(dendritic spine morphogenesis)|go:"GO:0097320"(plasma membrane tubulation)|go:"GO:0098794"(postsynapse)|go:"GO:0098974"(postsynaptic actin cytoskeleton organization)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903526"(negative regulation of membrane tubulation)|go:"GO:2000370"(positive regulation of clathrin-dependent endocytosis)|go:"GO:2000402"(negative regulation of lymphocyte migration)|go:"GO:2000601"(positive regulation of Arp2/3 complex-mediated actin nucleation)|interpro:IPR000095(PAK-box/P21-Rho-binding)|interpro:IPR000697(EVH1)|interpro:IPR003124(Actin-binding WH2)|interpro:IPR011026(Wiscott-Aldrich syndrome, C-terminal)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR030214|interpro:IPR033927|interpro:IPR036936|rcsb pdb:1MKE|rcsb pdb:2IFS|go:"GO:0000139"(Golgi membrane)	-	-	-	figure legend:fig. 4d|comment:"To further support the results from our Y2H assay, we decided to test the interaction of our cortactin constructs with N‐WASP. N‐WASP specifically interacted with the C‐terminus, but not with the N‐terminus of cortactin (Fig. 4D, grey bars). More importantly, N‐WASP was able to interact with Cort S/D, mimicking serine phosphorylation, but was almost completely unable to interact with the wild type or the construct mimicking tyrosine phosphorylation (Cort Y/D)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2019/12/02	2019/12/09	rogid:4hLyMrquWdlJnIX2pHuXqYtQgkE10090	rogid:lKgHtUQQEc8C14/X9D8WOvry2iM10116	rigid:HrrjCBXElXuLDNSKDWYcgjCHJTo	false	binding-associated region:488-546|mutation increasing interaction:421-421,466-466|mutation with no effect:525-525|mutation disrupting interaction:405-405,418-418	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q60598	uniprotkb:Q8X482	intact:EBI-397955|intact:EBI-4281621|uniprotkb:Q3UGC2|ensembl:ENSMUSP00000099368	intact:EBI-22229752|ensemblbacteria:AAG56991	psi-mi:src8_mouse(display_long)|uniprotkb:Cttn(gene name)|psi-mi:Cttn(display_short)|uniprotkb:Ems1(gene name synonym)	psi-mi:espfu_eco57(display_long)|uniprotkb:EspF-like protein encoded on prophage U(gene name synonym)|uniprotkb:Tir-cytoskeleton coupling protein TccP(gene name synonym)|uniprotkb:"espF(U)"(gene name)|psi-mi:"espF(U)"(display_short)|uniprotkb:tccP(gene name synonym)|uniprotkb:Z3072(locus name)	psi-mi:"MI:0018"(two hybrid)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22274767|imex:IM-27552-8	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_031829.2|ensembl:ENSMUSG00000031078(gene)|ensembl:ENSMUST00000103079(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030054"(cell junction)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051015"(actin filament binding)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|interpro:IPR036028|mint:Q60598|rcsb pdb:2F9X|rcsb pdb:3ULR|rcsb pdb:5NV1|rcsb pdb:5NVJ|rcsb pdb:5NXJ|reactome:R-MMU-8856828|dip:DIP-31562N	ensemblbacteria:AAG56991(transcript)|ensemblbacteria:Z3072(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0030430"(host cell cytoplasm)|mint:Q8X482|interpro:IPR006891(EspF protein)	-	-	-	figure legend:fig. 4d|comment:"the Y2H assay confirmed the interaction between EspFu and N‐WASP (top, white bar), as well as between EspFu and the C‐terminus of cortactin (black bars). More importantly, in accordance with the previous results, our Y2H assay showed a strong interaction between EspFu and Cort Y/D form, as compared with the wild‐type or Cort S/D mutant."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2019/12/02	2019/12/09	rogid:4hLyMrquWdlJnIX2pHuXqYtQgkE10090	rogid:5ta4O6gj+U5E7doG3zmjLy36nJ483334	rigid:9yuum9lt9eFKpJdH0OAI3CyXGpI	false	binding-associated region:488-546|mutation increasing interaction:421-421,466-466|mutation with no effect:405-405,418-418|mutation with no effect:525-525	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:O08816	uniprotkb:Q8X482	intact:EBI-6142604	intact:EBI-22229752|ensemblbacteria:AAG56991	psi-mi:wasl_rat(display_long)|uniprotkb:Wasl(gene name)|psi-mi:Wasl(display_short)	psi-mi:espfu_eco57(display_long)|uniprotkb:EspF-like protein encoded on prophage U(gene name synonym)|uniprotkb:Tir-cytoskeleton coupling protein TccP(gene name synonym)|uniprotkb:"espF(U)"(gene name)|psi-mi:"espF(U)"(display_short)|uniprotkb:tccP(gene name synonym)|uniprotkb:Z3072(locus name)	psi-mi:"MI:0018"(two hybrid)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22274805|imex:IM-27552-10	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	mint:O08816|rgd:735144|dip:DIP-17016N|go:"GO:0003779"(actin binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0008092"(cytoskeletal protein binding)|go:"GO:0009617"(response to bacterium)|go:"GO:0010324"(membrane invagination)|go:"GO:0016050"(vesicle organization)|go:"GO:0030027"(lamellipodium)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030048"(actin filament-based movement)|go:"GO:0030050"(vesicle transport along actin filament)|go:"GO:0030478"(actin cap)|go:"GO:0031252"(cell leading edge)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032880"(regulation of protein localization)|go:"GO:0034629"|go:"GO:0042802"(identical protein binding)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051301"(cell division)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0051653"(spindle localization)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0060997"(dendritic spine morphogenesis)|go:"GO:0097320"(plasma membrane tubulation)|go:"GO:0098794"(postsynapse)|go:"GO:0098974"(postsynaptic actin cytoskeleton organization)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903526"(negative regulation of membrane tubulation)|go:"GO:2000370"(positive regulation of clathrin-dependent endocytosis)|go:"GO:2000402"(negative regulation of lymphocyte migration)|go:"GO:2000601"(positive regulation of Arp2/3 complex-mediated actin nucleation)|interpro:IPR000095(PAK-box/P21-Rho-binding)|interpro:IPR000697(EVH1)|interpro:IPR003124(Actin-binding WH2)|interpro:IPR011026(Wiscott-Aldrich syndrome, C-terminal)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR030214|interpro:IPR033927|interpro:IPR036936|rcsb pdb:1MKE|rcsb pdb:2IFS|go:"GO:0000139"(Golgi membrane)	ensemblbacteria:AAG56991(transcript)|ensemblbacteria:Z3072(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0030430"(host cell cytoplasm)|mint:Q8X482|interpro:IPR006891(EspF protein)	-	-	-	figure legend:fig. 4d|comment:"the Y2H assay confirmed the interaction between EspFu and N‐WASP (top, white bar), as well as between EspFu and the C‐terminus of cortactin (black bars). More importantly, in accordance with the previous results, our Y2H assay showed a strong interaction between EspFu and Cort Y/D form, as compared with the wild‐type or Cort S/D mutant."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2019/12/02	2019/12/09	rogid:lKgHtUQQEc8C14/X9D8WOvry2iM10116	rogid:5ta4O6gj+U5E7doG3zmjLy36nJ483334	rigid:kpjsi7O6TBAKQFHiSDdtZHwSXD0	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q7DB77	uniprotkb:Q60598	intact:EBI-6480811|ensemblbacteria:AAG58825|ensemblbacteria:BAB37984|uniprotkb:Q7A9Q1|uniprotkb:Q9R396	intact:EBI-397955|intact:EBI-4281621|uniprotkb:Q3UGC2|ensembl:ENSMUSP00000099368	psi-mi:tir_eco57(display_long)|uniprotkb:tir(gene name)|psi-mi:tir(display_short)|uniprotkb:espE(gene name synonym)|uniprotkb:Secreted effector protein Tir(gene name synonym)|uniprotkb:Z5112(locus name)|uniprotkb:ECs4561(locus name)	psi-mi:src8_mouse(display_long)|uniprotkb:Cttn(gene name)|psi-mi:Cttn(display_short)|uniprotkb:Ems1(gene name synonym)	psi-mi:"MI:0018"(two hybrid)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22274982|imex:IM-27552-18	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	mint:Q7DB77|ensemblbacteria:AAG58825(transcript)|ensemblbacteria:BAB37984(transcript)|ensemblbacteria:ECs_4561(gene)|ensemblbacteria:Z5112(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0016021"(integral component of membrane)|go:"GO:0020002"(host cell plasma membrane)|rcsb pdb:2ZQK|rcsb pdb:2ZWK|refseq:WP_001301454.1|refseq:NP_312588.1|interpro:IPR022638|interpro:IPR022639|interpro:IPR003536(Translocated intimin receptor)|interpro:IPR022633|interpro:IPR037003	refseq:NP_031829.2|ensembl:ENSMUSG00000031078(gene)|ensembl:ENSMUST00000103079(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030054"(cell junction)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051015"(actin filament binding)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|interpro:IPR036028|mint:Q60598|rcsb pdb:2F9X|rcsb pdb:3ULR|rcsb pdb:5NV1|rcsb pdb:5NVJ|rcsb pdb:5NXJ|reactome:R-MMU-8856828|dip:DIP-31562N	-	-	-	figure legend:fig. 5d|comment:"the Y2H assay confirmed the interaction between EspFu and N‐WASP (top, white bar), as well as between EspFu and the C‐terminus of cortactin (black bars). More importantly, in accordance with the previous results, our Y2H assay showed a strong interaction between EspFu and Cort Y/D form, as compared with the wild‐type or Cort S/D mutant."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2019/12/02	2019/12/09	rogid:w091zQ2yloHiTr07621yQtr7n0w83334	rogid:4hLyMrquWdlJnIX2pHuXqYtQgkE10090	rigid:hy21jRD8pgXiayiN2bTNc/HYd8g	false	binding-associated region:1-172	binding-associated region:488-546|mutation increasing interaction:421-421,466-466|mutation with no effect:405-405,418-418	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q8X482	uniprotkb:Q60598	intact:EBI-22229752|ensemblbacteria:AAG56991	intact:EBI-397955|intact:EBI-4281621|uniprotkb:Q3UGC2|ensembl:ENSMUSP00000099368	psi-mi:espfu_eco57(display_long)|uniprotkb:EspF-like protein encoded on prophage U(gene name synonym)|uniprotkb:Tir-cytoskeleton coupling protein TccP(gene name synonym)|uniprotkb:"espF(U)"(gene name)|psi-mi:"espF(U)"(display_short)|uniprotkb:tccP(gene name synonym)|uniprotkb:Z3072(locus name)	psi-mi:src8_mouse(display_long)|uniprotkb:Cttn(gene name)|psi-mi:Cttn(display_short)|uniprotkb:Ems1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22272877|imex:IM-27552-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG56991(transcript)|ensemblbacteria:Z3072(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0030430"(host cell cytoplasm)|mint:Q8X482|interpro:IPR006891(EspF protein)	refseq:NP_031829.2|ensembl:ENSMUSG00000031078(gene)|ensembl:ENSMUST00000103079(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030054"(cell junction)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051015"(actin filament binding)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|interpro:IPR036028|mint:Q60598|rcsb pdb:2F9X|rcsb pdb:3ULR|rcsb pdb:5NV1|rcsb pdb:5NVJ|rcsb pdb:5NXJ|reactome:R-MMU-8856828|dip:DIP-31562N	-	-	-	figure legend:fig. 4b|comment:To confirm these findings further, we used lysates obtained from HeLa cells and D5 cells supplemented with purified His‐EspFu‐myc. HeLa cell lysates contain very low levels of tyrosine‐phosphorylated cortactin, in contrast to D5 lysates. As shown in Fig. 4B, tyrosine‐phosphorylated cortactin co‐immunoprecipitates with His‐EspFu‐myc from D5 cells lysates, and no cortactin was co‐immunoprecipitated from the HeLa cells lysates, strongly suggesting that EspFu specifically binds to the tyrosine‐phosphorylated form of cortactin.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/12/02	2019/12/05	rogid:5ta4O6gj+U5E7doG3zmjLy36nJ483334	rogid:4hLyMrquWdlJnIX2pHuXqYtQgkE10090	rigid:9yuum9lt9eFKpJdH0OAI3CyXGpI	false	myc tag:c-c|his tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q60598	uniprotkb:Q7DB77	intact:EBI-397955|intact:EBI-4281621|uniprotkb:Q3UGC2|ensembl:ENSMUSP00000099368	intact:EBI-6480811|ensemblbacteria:AAG58825|ensemblbacteria:BAB37984|uniprotkb:Q7A9Q1|uniprotkb:Q9R396	psi-mi:src8_mouse(display_long)|uniprotkb:Cttn(gene name)|psi-mi:Cttn(display_short)|uniprotkb:Ems1(gene name synonym)	psi-mi:tir_eco57(display_long)|uniprotkb:tir(gene name)|psi-mi:tir(display_short)|uniprotkb:espE(gene name synonym)|uniprotkb:Secreted effector protein Tir(gene name synonym)|uniprotkb:Z5112(locus name)|uniprotkb:ECs4561(locus name)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-22274926|imex:IM-27552-14	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_031829.2|ensembl:ENSMUSG00000031078(gene)|ensembl:ENSMUST00000103079(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030054"(cell junction)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051015"(actin filament binding)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|interpro:IPR036028|mint:Q60598|rcsb pdb:2F9X|rcsb pdb:3ULR|rcsb pdb:5NV1|rcsb pdb:5NVJ|rcsb pdb:5NXJ|reactome:R-MMU-8856828|dip:DIP-31562N	mint:Q7DB77|ensemblbacteria:AAG58825(transcript)|ensemblbacteria:BAB37984(transcript)|ensemblbacteria:ECs_4561(gene)|ensemblbacteria:Z5112(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0016021"(integral component of membrane)|go:"GO:0020002"(host cell plasma membrane)|rcsb pdb:2ZQK|rcsb pdb:2ZWK|refseq:WP_001301454.1|refseq:NP_312588.1|interpro:IPR022638|interpro:IPR022639|interpro:IPR003536(Translocated intimin receptor)|interpro:IPR022633|interpro:IPR037003	-	-	-	figure legend:fig. 5b|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/12/02	2019/12/09	rogid:4hLyMrquWdlJnIX2pHuXqYtQgkE10090	rogid:w091zQ2yloHiTr07621yQtr7n0w83334	rigid:hy21jRD8pgXiayiN2bTNc/HYd8g	false	phosphorylated residue:421-421	his tag:?-?|binding-associated region:1-172	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q8X482	uniprotkb:Q60598	intact:EBI-22229752|ensemblbacteria:AAG56991	intact:EBI-397955|intact:EBI-4281621|uniprotkb:Q3UGC2|ensembl:ENSMUSP00000099368	psi-mi:espfu_eco57(display_long)|uniprotkb:EspF-like protein encoded on prophage U(gene name synonym)|uniprotkb:Tir-cytoskeleton coupling protein TccP(gene name synonym)|uniprotkb:"espF(U)"(gene name)|psi-mi:"espF(U)"(display_short)|uniprotkb:tccP(gene name synonym)|uniprotkb:Z3072(locus name)	psi-mi:src8_mouse(display_long)|uniprotkb:Cttn(gene name)|psi-mi:Cttn(display_short)|uniprotkb:Ems1(gene name synonym)	psi-mi:"MI:0416"(fluorescence microscopy)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-22275012|imex:IM-27552-22	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG56991(transcript)|ensemblbacteria:Z3072(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0030430"(host cell cytoplasm)|mint:Q8X482|interpro:IPR006891(EspF protein)	refseq:NP_031829.2|ensembl:ENSMUSG00000031078(gene)|ensembl:ENSMUST00000103079(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030054"(cell junction)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051015"(actin filament binding)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|interpro:IPR036028|mint:Q60598|rcsb pdb:2F9X|rcsb pdb:3ULR|rcsb pdb:5NV1|rcsb pdb:5NVJ|rcsb pdb:5NXJ|reactome:R-MMU-8856828|dip:DIP-31562N	go:"GO:0042995"(cell projection)	-	-	figure legend:suppl fig 1b|comment:"when expressed in non‐infected D5 cells, EspFu‐FLAG was found to accumulate in tyrosine‐phosphorylated cortactin‐rich structures (Fig. S1B), again supporting the idea that EspFu associates with tyrosine‐phosphorylated cortactin inside the host cells."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-fibroblast)|taxid:10090(Mus musculus)	-	2019/12/02	2019/12/09	rogid:5ta4O6gj+U5E7doG3zmjLy36nJ483334	rogid:4hLyMrquWdlJnIX2pHuXqYtQgkE10090	rigid:9yuum9lt9eFKpJdH0OAI3CyXGpI	false	flag tag:?-?	-	-	-	psi-mi:"MI:0707"(anti tag immunostaining)	psi-mi:"MI:0422"(immunostaining)
uniprotkb:Q60598	uniprotkb:Q8X482	intact:EBI-397955|intact:EBI-4281621|uniprotkb:Q3UGC2|ensembl:ENSMUSP00000099368	intact:EBI-22229752|ensemblbacteria:AAG56991	psi-mi:src8_mouse(display_long)|uniprotkb:Cttn(gene name)|psi-mi:Cttn(display_short)|uniprotkb:Ems1(gene name synonym)	psi-mi:espfu_eco57(display_long)|uniprotkb:EspF-like protein encoded on prophage U(gene name synonym)|uniprotkb:Tir-cytoskeleton coupling protein TccP(gene name synonym)|uniprotkb:"espF(U)"(gene name)|psi-mi:"espF(U)"(display_short)|uniprotkb:tccP(gene name synonym)|uniprotkb:Z3072(locus name)	psi-mi:"MI:0416"(fluorescence microscopy)	Cantarelli et al. (2007)	imex:IM-27552|pubmed:17451412	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-22275030|imex:IM-27552-24	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_031829.2|ensembl:ENSMUSG00000031078(gene)|ensembl:ENSMUST00000103079(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030054"(cell junction)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051015"(actin filament binding)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|interpro:IPR036028|mint:Q60598|rcsb pdb:2F9X|rcsb pdb:3ULR|rcsb pdb:5NV1|rcsb pdb:5NVJ|rcsb pdb:5NXJ|reactome:R-MMU-8856828|dip:DIP-31562N	ensemblbacteria:AAG56991(transcript)|ensemblbacteria:Z3072(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0030430"(host cell cytoplasm)|mint:Q8X482|interpro:IPR006891(EspF protein)	go:"GO:0042995"(cell projection)	-	-	figure legend:supll fig 2b|comment:"Infection of HeLa cells with DMEM‐activated EHEC expressing FLAG‐tagged EspFu (as in Fig. 2C and D) resulted in a perfect colocalization of EspFu‐FLAG and tyrosine‐phosphorylated cortactin, which was visible 45 min after infection (Fig. 4C). As the infection proceeded (120 min), cortactin was dephosphorylated and only EspFu‐FLAG was detected near the bacteria."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10090(mouse-fibroblast)|taxid:10090(Mus musculus)	-	2019/12/02	2019/12/09	rogid:4hLyMrquWdlJnIX2pHuXqYtQgkE10090	rogid:5ta4O6gj+U5E7doG3zmjLy36nJ483334	rigid:9yuum9lt9eFKpJdH0OAI3CyXGpI	false	-	green fluorescent protein tag:?-?	-	-	psi-mi:"MI:0422"(immunostaining)	psi-mi:"MI:0707"(anti tag immunostaining)