#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
intact:EBI-11701537	uniprotkb:P04802	rnacentral:URS000027E1E5_559292	intact:EBI-18655|uniprotkb:D6VXY6|ensemblfungi:YLL018C	psi-mi:trna_asp_guc(display_short)|psi-mi:EBI-11701537(display_long)	psi-mi:sydc_yeast(display_long)|uniprotkb:DPS1(gene name)|psi-mi:DPS1(display_short)|uniprotkb:APS(gene name synonym)|uniprotkb:APS1(gene name synonym)|uniprotkb:Aspartyl-tRNA synthetase(gene name synonym)|uniprotkb:L1295(orf name)|uniprotkb:YLL018C(locus name)	psi-mi:"MI:0114"(x-ray crystallography)	Cavarelli et al. (1994)	pubmed:8313877|imex:IM-26587	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-12561366|imex:IM-26587-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0325"(transfer rna)	psi-mi:"MI:0326"(protein)	-	refseq:NP_013083.1|sgd:S000003941|ensemblfungi:YLL018C(gene)|ensemblfungi:YLL018C_mRNA(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0004815"(aspartate-tRNA ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006422"(aspartyl-tRNA aminoacylation)|go:"GO:0017101"(aminoacyl-tRNA synthetase multienzyme complex)|interpro:IPR002312(Aspartyl-tRNA synthetase, class IIb)|interpro:IPR004364("Aminoacyl-tRNA synthetase, class II (D, K and N)")|interpro:IPR004365(Nucleic acid binding, OB-fold, tRNA/helicase-type)|interpro:IPR004523(Aspartyl-tRNA synthetase, class IIb, archea/euk type)|interpro:IPR006195(Aminoacyl-tRNA synthetase, class II)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|rcsb pdb:1ASY|rcsb pdb:1ASZ|rcsb pdb:1EOV|dip:DIP-4093N|mint:P04802	-	-	crc64:6656279B3E9011A5	figure legend:Table 1|3d-r-factors:0.225|3d-resolution:2.7|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/18	2016/10/18	-	rogid:Rr9kHQcG78vfO3DYbDL+FDBevUw559292	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:22660"	chebi:"CHEBI:15422"	intact:EBI-12561617	intact:EBI-1108845|chebi:"CHEBI:40938"|chebi:"CHEBI:10841"|chebi:"CHEBI:10789"|chebi:"CHEBI:2359"|chebi:"CHEBI:13236"|chebi:"CHEBI:22249"	psi-mi:aspartic acid(display_short)|psi-mi:"CHEBI:22660"(display_long)|intact:"(+-)-Aspartic acid"(synonym)|intact:"(R,S)-Aspartic acid"(synonym)|intact:2-aminobutanedioic acid(synonym)|intact:Asp(synonym)|intact:Aspartic acid(synonym)|intact:D(synonym)|intact:DL-Aminosuccinic acid(synonym)|intact:DL-Asparagic acid(synonym)|intact:aspartic acid(iupac name)	psi-mi:atp(display_short)|psi-mi:"CHEBI:15422"(display_long)|intact:Adenosine 5'-triphosphate(synonym)|intact:Adenosine triphosphate(synonym)|intact:ADENOSINE-5'-TRIPHOSPHATE(synonym)|intact:Adephos(synonym)|intact:Adetol(synonym)|intact:Adynol(synonym)|intact:Atipi(synonym)|intact:ATP(synonym)|intact:Atriphos(synonym)|intact:Cardenosine(synonym)|intact:Fosfobion(synonym)|intact:Glucobasin(synonym)|intact:H4atp(synonym)|intact:Myotriphos(synonym)|intact:Triadenyl(synonym)|intact:Triphosphaden(synonym)|intact:"adenosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:"MI:0114"(x-ray crystallography)	Cavarelli et al. (1994)	pubmed:8313877|imex:IM-26587	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-12561609|imex:IM-26587-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0328"(small molecule)	-	-	-	-	-	figure legend:Table 1|3d-r-factors:0.212|3d-resolution:3.0|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/18	2017/02/27	-	-	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:22660"	uniprotkb:P04802	intact:EBI-12561617	intact:EBI-18655|uniprotkb:D6VXY6|ensemblfungi:YLL018C	psi-mi:aspartic acid(display_short)|psi-mi:"CHEBI:22660"(display_long)|intact:"(+-)-Aspartic acid"(synonym)|intact:"(R,S)-Aspartic acid"(synonym)|intact:2-aminobutanedioic acid(synonym)|intact:Asp(synonym)|intact:Aspartic acid(synonym)|intact:D(synonym)|intact:DL-Aminosuccinic acid(synonym)|intact:DL-Asparagic acid(synonym)|intact:aspartic acid(iupac name)	psi-mi:sydc_yeast(display_long)|uniprotkb:DPS1(gene name)|psi-mi:DPS1(display_short)|uniprotkb:APS(gene name synonym)|uniprotkb:APS1(gene name synonym)|uniprotkb:Aspartyl-tRNA synthetase(gene name synonym)|uniprotkb:L1295(orf name)|uniprotkb:YLL018C(locus name)	psi-mi:"MI:0114"(x-ray crystallography)	Cavarelli et al. (1994)	pubmed:8313877|imex:IM-26587	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-12561609|imex:IM-26587-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:NP_013083.1|sgd:S000003941|ensemblfungi:YLL018C(gene)|ensemblfungi:YLL018C_mRNA(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0004815"(aspartate-tRNA ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006422"(aspartyl-tRNA aminoacylation)|go:"GO:0017101"(aminoacyl-tRNA synthetase multienzyme complex)|interpro:IPR002312(Aspartyl-tRNA synthetase, class IIb)|interpro:IPR004364("Aminoacyl-tRNA synthetase, class II (D, K and N)")|interpro:IPR004365(Nucleic acid binding, OB-fold, tRNA/helicase-type)|interpro:IPR004523(Aspartyl-tRNA synthetase, class IIb, archea/euk type)|interpro:IPR006195(Aminoacyl-tRNA synthetase, class II)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|rcsb pdb:1ASY|rcsb pdb:1ASZ|rcsb pdb:1EOV|dip:DIP-4093N|mint:P04802	-	-	crc64:6656279B3E9011A5	figure legend:Table 1|3d-r-factors:0.212|3d-resolution:3.0|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/18	2017/02/27	-	rogid:Rr9kHQcG78vfO3DYbDL+FDBevUw559292	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:22660"	intact:EBI-11701537	intact:EBI-12561617	rnacentral:URS000027E1E5_559292	psi-mi:aspartic acid(display_short)|psi-mi:"CHEBI:22660"(display_long)|intact:"(+-)-Aspartic acid"(synonym)|intact:"(R,S)-Aspartic acid"(synonym)|intact:2-aminobutanedioic acid(synonym)|intact:Asp(synonym)|intact:Aspartic acid(synonym)|intact:D(synonym)|intact:DL-Aminosuccinic acid(synonym)|intact:DL-Asparagic acid(synonym)|intact:aspartic acid(iupac name)	psi-mi:trna_asp_guc(display_short)|psi-mi:EBI-11701537(display_long)	psi-mi:"MI:0114"(x-ray crystallography)	Cavarelli et al. (1994)	pubmed:8313877|imex:IM-26587	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-12561609|imex:IM-26587-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0325"(transfer rna)	-	-	-	-	-	figure legend:Table 1|3d-r-factors:0.212|3d-resolution:3.0|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/18	2017/02/27	-	-	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15422"	uniprotkb:P04802	intact:EBI-1108845|chebi:"CHEBI:40938"|chebi:"CHEBI:10841"|chebi:"CHEBI:10789"|chebi:"CHEBI:2359"|chebi:"CHEBI:13236"|chebi:"CHEBI:22249"	intact:EBI-18655|uniprotkb:D6VXY6|ensemblfungi:YLL018C	psi-mi:atp(display_short)|psi-mi:"CHEBI:15422"(display_long)|intact:Adenosine 5'-triphosphate(synonym)|intact:Adenosine triphosphate(synonym)|intact:ADENOSINE-5'-TRIPHOSPHATE(synonym)|intact:Adephos(synonym)|intact:Adetol(synonym)|intact:Adynol(synonym)|intact:Atipi(synonym)|intact:ATP(synonym)|intact:Atriphos(synonym)|intact:Cardenosine(synonym)|intact:Fosfobion(synonym)|intact:Glucobasin(synonym)|intact:H4atp(synonym)|intact:Myotriphos(synonym)|intact:Triadenyl(synonym)|intact:Triphosphaden(synonym)|intact:"adenosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:sydc_yeast(display_long)|uniprotkb:DPS1(gene name)|psi-mi:DPS1(display_short)|uniprotkb:APS(gene name synonym)|uniprotkb:APS1(gene name synonym)|uniprotkb:Aspartyl-tRNA synthetase(gene name synonym)|uniprotkb:L1295(orf name)|uniprotkb:YLL018C(locus name)	psi-mi:"MI:0114"(x-ray crystallography)	Cavarelli et al. (1994)	pubmed:8313877|imex:IM-26587	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13646161|wwpdb:1ASZ|imex:IM-26587-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:NP_013083.1|sgd:S000003941|ensemblfungi:YLL018C(gene)|ensemblfungi:YLL018C_mRNA(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0004815"(aspartate-tRNA ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006422"(aspartyl-tRNA aminoacylation)|go:"GO:0017101"(aminoacyl-tRNA synthetase multienzyme complex)|interpro:IPR002312(Aspartyl-tRNA synthetase, class IIb)|interpro:IPR004364("Aminoacyl-tRNA synthetase, class II (D, K and N)")|interpro:IPR004365(Nucleic acid binding, OB-fold, tRNA/helicase-type)|interpro:IPR004523(Aspartyl-tRNA synthetase, class IIb, archea/euk type)|interpro:IPR006195(Aminoacyl-tRNA synthetase, class II)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|rcsb pdb:1ASY|rcsb pdb:1ASZ|rcsb pdb:1EOV|dip:DIP-4093N|mint:P04802	-	-	crc64:6656279B3E9011A5	figure legend:Table 1|3d-r-factors:0.203|3d-resolution:3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/18	2017/02/15	-	rogid:Rr9kHQcG78vfO3DYbDL+FDBevUw559292	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15422"	intact:EBI-11701537	intact:EBI-1108845|chebi:"CHEBI:40938"|chebi:"CHEBI:10841"|chebi:"CHEBI:10789"|chebi:"CHEBI:2359"|chebi:"CHEBI:13236"|chebi:"CHEBI:22249"	rnacentral:URS000027E1E5_559292	psi-mi:atp(display_short)|psi-mi:"CHEBI:15422"(display_long)|intact:Adenosine 5'-triphosphate(synonym)|intact:Adenosine triphosphate(synonym)|intact:ADENOSINE-5'-TRIPHOSPHATE(synonym)|intact:Adephos(synonym)|intact:Adetol(synonym)|intact:Adynol(synonym)|intact:Atipi(synonym)|intact:ATP(synonym)|intact:Atriphos(synonym)|intact:Cardenosine(synonym)|intact:Fosfobion(synonym)|intact:Glucobasin(synonym)|intact:H4atp(synonym)|intact:Myotriphos(synonym)|intact:Triadenyl(synonym)|intact:Triphosphaden(synonym)|intact:"adenosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:trna_asp_guc(display_short)|psi-mi:EBI-11701537(display_long)	psi-mi:"MI:0114"(x-ray crystallography)	Cavarelli et al. (1994)	pubmed:8313877|imex:IM-26587	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13646161|wwpdb:1ASZ|imex:IM-26587-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0325"(transfer rna)	-	-	-	-	-	figure legend:Table 1|3d-r-factors:0.203|3d-resolution:3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/18	2017/02/15	-	-	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:22660"	uniprotkb:P04802	intact:EBI-12561617	intact:EBI-18655|uniprotkb:D6VXY6|ensemblfungi:YLL018C	psi-mi:aspartic acid(display_short)|psi-mi:"CHEBI:22660"(display_long)|intact:"(+-)-Aspartic acid"(synonym)|intact:"(R,S)-Aspartic acid"(synonym)|intact:2-aminobutanedioic acid(synonym)|intact:Asp(synonym)|intact:Aspartic acid(synonym)|intact:D(synonym)|intact:DL-Aminosuccinic acid(synonym)|intact:DL-Asparagic acid(synonym)|intact:aspartic acid(iupac name)	psi-mi:sydc_yeast(display_long)|uniprotkb:DPS1(gene name)|psi-mi:DPS1(display_short)|uniprotkb:APS(gene name synonym)|uniprotkb:APS1(gene name synonym)|uniprotkb:Aspartyl-tRNA synthetase(gene name synonym)|uniprotkb:L1295(orf name)|uniprotkb:YLL018C(locus name)	psi-mi:"MI:0415"(enzymatic study)	Cavarelli et al. (1994)	pubmed:8313877|imex:IM-26587	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-12561638|imex:IM-26587-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:NP_013083.1|sgd:S000003941|ensemblfungi:YLL018C(gene)|ensemblfungi:YLL018C_mRNA(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0004815"(aspartate-tRNA ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006422"(aspartyl-tRNA aminoacylation)|go:"GO:0017101"(aminoacyl-tRNA synthetase multienzyme complex)|interpro:IPR002312(Aspartyl-tRNA synthetase, class IIb)|interpro:IPR004364("Aminoacyl-tRNA synthetase, class II (D, K and N)")|interpro:IPR004365(Nucleic acid binding, OB-fold, tRNA/helicase-type)|interpro:IPR004523(Aspartyl-tRNA synthetase, class IIb, archea/euk type)|interpro:IPR006195(Aminoacyl-tRNA synthetase, class II)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|rcsb pdb:1ASY|rcsb pdb:1ASZ|rcsb pdb:1EOV|dip:DIP-4093N|mint:P04802	go:"GO:0004815"(aspartate-tRNA ligase activity)|go:"GO:0006422"(aspartyl-tRNA aminoacylation)	-	crc64:6656279B3E9011A5	figure legend:Table  3|comment:"Authors classify residues potentially relevant for the reaction in 3 groups and identify by extensive mutagenesis analysis the essential ones, listed below:   Group 1, residues that contact ATP: Arg325, Asp471, Glu478  Group 2, residues that contact amino acid: Gln303, Asp342, Arg485, Glu344, Lys306  Group 3, residues that contact tRNA: Ser301 Glu327, His334  However, individually all these residues contribute only weakly to the tRNA-enzyme binding energy, with little changes in Kd"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	km:25.0x10^-4(molar)|kd:3.0x10^-8(molar)|kcat:83.0x10^-2(second -1)	2016/10/18	2017/03/08	-	rogid:Rr9kHQcG78vfO3DYbDL+FDBevUw559292	-	false	-	mutation decreasing interaction:303-303|mutation decreasing interaction:334-334|mutation decreasing interaction:329-329|mutation decreasing interaction:334-334	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:22660"	intact:EBI-11701537	intact:EBI-12561617	rnacentral:URS000027E1E5_559292	psi-mi:aspartic acid(display_short)|psi-mi:"CHEBI:22660"(display_long)|intact:"(+-)-Aspartic acid"(synonym)|intact:"(R,S)-Aspartic acid"(synonym)|intact:2-aminobutanedioic acid(synonym)|intact:Asp(synonym)|intact:Aspartic acid(synonym)|intact:D(synonym)|intact:DL-Aminosuccinic acid(synonym)|intact:DL-Asparagic acid(synonym)|intact:aspartic acid(iupac name)	psi-mi:trna_asp_guc(display_short)|psi-mi:EBI-11701537(display_long)	psi-mi:"MI:0415"(enzymatic study)	Cavarelli et al. (1994)	pubmed:8313877|imex:IM-26587	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-12561638|imex:IM-26587-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0325"(transfer rna)	-	-	go:"GO:0004815"(aspartate-tRNA ligase activity)|go:"GO:0006422"(aspartyl-tRNA aminoacylation)	-	-	figure legend:Table  3|comment:"Authors classify residues potentially relevant for the reaction in 3 groups and identify by extensive mutagenesis analysis the essential ones, listed below:   Group 1, residues that contact ATP: Arg325, Asp471, Glu478  Group 2, residues that contact amino acid: Gln303, Asp342, Arg485, Glu344, Lys306  Group 3, residues that contact tRNA: Ser301 Glu327, His334  However, individually all these residues contribute only weakly to the tRNA-enzyme binding energy, with little changes in Kd"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	km:25.0x10^-4(molar)|kd:3.0x10^-8(molar)|kcat:83.0x10^-2(second -1)	2016/10/18	2017/03/08	-	-	-	false	-	32p radiolabel:?-?	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)