#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:Q03774 uniprotkb:Q12009 intact:EBI-19486|uniprotkb:D6VSE5|ensemblfungi:YDR165W intact:EBI-19552|uniprotkb:D6VRF3|ensemblfungi:YDL201W psi-mi:trm82_yeast(display_long)|uniprotkb:TRM82(gene name)|psi-mi:TRM82(display_short)|uniprotkb:YDR165W(locus name)|uniprotkb:YD8358.19(orf name)|uniprotkb:Transfer RNA methyltransferase 82(gene name synonym) psi-mi:trmb_yeast(display_long)|uniprotkb:"tRNA (guanine(46)-N(7))-methyltransferase"(gene name synonym)|uniprotkb:TRM8(gene name)|psi-mi:TRM8(display_short)|uniprotkb:YDL201W(locus name)|uniprotkb:D1075(orf name)|uniprotkb:"tRNA(m7G46)-methyltransferase"(gene name synonym)|uniprotkb:Transfer RNA methyltransferase 8(gene name synonym) psi-mi:"MI:0515"(methyltransferase assay) Alexandrov et al. (2002) pubmed:12403464|imex:IM-26751 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0213"(methylation reaction) psi-mi:"MI:0469"(IntAct) intact:EBI-12519061|imex:IM-26751-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:1343"(enzyme regulator) psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_010449.1|sgd:S000002572|ensemblfungi:YDR165W(gene)|ensemblfungi:YDR165W_mRNA(transcript)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0043527"(tRNA methyltransferase complex)|go:"GO:0106004"("tRNA (guanine-N7)-methylation")|go:"GO:0106143"("tRNA (m7G46) methyltransferase complex")|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR028884|interpro:IPR036322|rcsb pdb:2VDU|dip:DIP-8795N|mint:Q03774 refseq:NP_010080.1|sgd:S000002360|ensemblfungi:YDL201W(gene)|ensemblfungi:YDL201W_mRNA(transcript)|go:"GO:0000049"(tRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0008176"("tRNA (guanine-N7-)-methyltransferase activity")|go:"GO:0030488"(tRNA methylation)|go:"GO:0036265"("RNA (guanine-N7)-methylation")|go:"GO:0043527"(tRNA methyltransferase complex)|go:"GO:0106004"("tRNA (guanine-N7)-methylation")|go:"GO:0106143"("tRNA (m7G46) methyltransferase complex")|interpro:IPR003358(Putative methyltransferase)|interpro:IPR025763|interpro:IPR029063|rcsb pdb:2VDU|rcsb pdb:2VDV|dip:DIP-8614N|mint:Q12009 go:"GO:0008176"("tRNA (guanine-N7-)-methyltransferase activity") crc64:F1E0847F44376969 crc64:0280B9A7B02C85B7 figure legend:Fig 7 B|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2016/09/13 2016/09/28 rogid:YRWUfQr4Jlzws67uFucIXn9XQGI559292 rogid:06ACXIJdYzcgtDlZwCmDsidozno559292 - false tandem tag:n-n tandem tag:n-n - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:Q03774 intact:EBI-11705578 intact:EBI-19486|uniprotkb:D6VSE5|ensemblfungi:YDR165W rnacentral:URS00000890D0_559292 psi-mi:trm82_yeast(display_long)|uniprotkb:TRM82(gene name)|psi-mi:TRM82(display_short)|uniprotkb:YDR165W(locus name)|uniprotkb:YD8358.19(orf name)|uniprotkb:Transfer RNA methyltransferase 82(gene name synonym) psi-mi:trna phe gaa precursor(display_short)|psi-mi:EBI-11705578(display_long) psi-mi:"MI:0515"(methyltransferase assay) Alexandrov et al. (2002) pubmed:12403464|imex:IM-26751 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0213"(methylation reaction) psi-mi:"MI:0469"(IntAct) intact:EBI-12519061|imex:IM-26751-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:1343"(enzyme regulator) psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0325"(transfer rna) refseq:NP_010449.1|sgd:S000002572|ensemblfungi:YDR165W(gene)|ensemblfungi:YDR165W_mRNA(transcript)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0043527"(tRNA methyltransferase complex)|go:"GO:0106004"("tRNA (guanine-N7)-methylation")|go:"GO:0106143"("tRNA (m7G46) methyltransferase complex")|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR028884|interpro:IPR036322|rcsb pdb:2VDU|dip:DIP-8795N|mint:Q03774 - go:"GO:0008176"("tRNA (guanine-N7-)-methyltransferase activity") crc64:F1E0847F44376969 - figure legend:Fig 7 B|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2016/09/13 2016/09/28 rogid:YRWUfQr4Jlzws67uFucIXn9XQGI559292 - - false tandem tag:n-n 32p radiolabel:?-?|RNA chemical modification:?-?("CHEBI:20794") - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:Q03774 uniprotkb:Q12009 intact:EBI-19486|uniprotkb:D6VSE5|ensemblfungi:YDR165W intact:EBI-19552|uniprotkb:D6VRF3|ensemblfungi:YDL201W psi-mi:trm82_yeast(display_long)|uniprotkb:TRM82(gene name)|psi-mi:TRM82(display_short)|uniprotkb:YDR165W(locus name)|uniprotkb:YD8358.19(orf name)|uniprotkb:Transfer RNA methyltransferase 82(gene name synonym) psi-mi:trmb_yeast(display_long)|uniprotkb:"tRNA (guanine(46)-N(7))-methyltransferase"(gene name synonym)|uniprotkb:TRM8(gene name)|psi-mi:TRM8(display_short)|uniprotkb:YDL201W(locus name)|uniprotkb:D1075(orf name)|uniprotkb:"tRNA(m7G46)-methyltransferase"(gene name synonym)|uniprotkb:Transfer RNA methyltransferase 8(gene name synonym) psi-mi:"MI:0515"(methyltransferase assay) Alexandrov et al. (2002) pubmed:12403464|imex:IM-26751 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0213"(methylation reaction) psi-mi:"MI:0469"(IntAct) intact:EBI-12519457|imex:IM-26751-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:1343"(enzyme regulator) psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_010449.1|sgd:S000002572|ensemblfungi:YDR165W(gene)|ensemblfungi:YDR165W_mRNA(transcript)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0043527"(tRNA methyltransferase complex)|go:"GO:0106004"("tRNA (guanine-N7)-methylation")|go:"GO:0106143"("tRNA (m7G46) methyltransferase complex")|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR028884|interpro:IPR036322|rcsb pdb:2VDU|dip:DIP-8795N|mint:Q03774 refseq:NP_010080.1|sgd:S000002360|ensemblfungi:YDL201W(gene)|ensemblfungi:YDL201W_mRNA(transcript)|go:"GO:0000049"(tRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0008176"("tRNA (guanine-N7-)-methyltransferase activity")|go:"GO:0030488"(tRNA methylation)|go:"GO:0036265"("RNA (guanine-N7)-methylation")|go:"GO:0043527"(tRNA methyltransferase complex)|go:"GO:0106004"("tRNA (guanine-N7)-methylation")|go:"GO:0106143"("tRNA (m7G46) methyltransferase complex")|interpro:IPR003358(Putative methyltransferase)|interpro:IPR025763|interpro:IPR029063|rcsb pdb:2VDU|rcsb pdb:2VDV|dip:DIP-8614N|mint:Q12009 go:"GO:0008176"("tRNA (guanine-N7-)-methyltransferase activity") crc64:F1E0847F44376969 crc64:0280B9A7B02C85B7 figure legend:Fig 8|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2016/09/13 2016/09/28 rogid:YRWUfQr4Jlzws67uFucIXn9XQGI559292 rogid:06ACXIJdYzcgtDlZwCmDsidozno559292 - false tandem tag:n-n tandem tag:n-n - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:Q03774 intact:EBI-11705578 intact:EBI-19486|uniprotkb:D6VSE5|ensemblfungi:YDR165W rnacentral:URS00000890D0_559292 psi-mi:trm82_yeast(display_long)|uniprotkb:TRM82(gene name)|psi-mi:TRM82(display_short)|uniprotkb:YDR165W(locus name)|uniprotkb:YD8358.19(orf name)|uniprotkb:Transfer RNA methyltransferase 82(gene name synonym) psi-mi:trna phe gaa precursor(display_short)|psi-mi:EBI-11705578(display_long) psi-mi:"MI:0515"(methyltransferase assay) Alexandrov et al. (2002) pubmed:12403464|imex:IM-26751 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0213"(methylation reaction) psi-mi:"MI:0469"(IntAct) intact:EBI-12519457|imex:IM-26751-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:1343"(enzyme regulator) psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0325"(transfer rna) refseq:NP_010449.1|sgd:S000002572|ensemblfungi:YDR165W(gene)|ensemblfungi:YDR165W_mRNA(transcript)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0043527"(tRNA methyltransferase complex)|go:"GO:0106004"("tRNA (guanine-N7)-methylation")|go:"GO:0106143"("tRNA (m7G46) methyltransferase complex")|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR028884|interpro:IPR036322|rcsb pdb:2VDU|dip:DIP-8795N|mint:Q03774 - go:"GO:0008176"("tRNA (guanine-N7-)-methyltransferase activity") crc64:F1E0847F44376969 comment:To identify the methylated position, the RNA was subjected to borohydride reduction followed by aniline cleavage of the RNA figure legend:Fig 8|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2016/09/13 2016/09/28 rogid:YRWUfQr4Jlzws67uFucIXn9XQGI559292 - - false tandem tag:n-n RNA chemical modification:64-64("CHEBI:20794")|32p radiolabel:91-91 - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:Q03774 uniprotkb:Q12009 intact:EBI-19486|uniprotkb:D6VSE5|ensemblfungi:YDR165W intact:EBI-19552|uniprotkb:D6VRF3|ensemblfungi:YDL201W psi-mi:trm82_yeast(display_long)|uniprotkb:TRM82(gene name)|psi-mi:TRM82(display_short)|uniprotkb:YDR165W(locus name)|uniprotkb:YD8358.19(orf name)|uniprotkb:Transfer RNA methyltransferase 82(gene name synonym) psi-mi:trmb_yeast(display_long)|uniprotkb:"tRNA (guanine(46)-N(7))-methyltransferase"(gene name synonym)|uniprotkb:TRM8(gene name)|psi-mi:TRM8(display_short)|uniprotkb:YDL201W(locus name)|uniprotkb:D1075(orf name)|uniprotkb:"tRNA(m7G46)-methyltransferase"(gene name synonym)|uniprotkb:Transfer RNA methyltransferase 8(gene name synonym) psi-mi:"MI:0004"(affinity chromatography technology) Alexandrov et al. (2002) pubmed:12403464|imex:IM-26751 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-12519542|imex:IM-26751-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_010449.1|sgd:S000002572|ensemblfungi:YDR165W(gene)|ensemblfungi:YDR165W_mRNA(transcript)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0043527"(tRNA methyltransferase complex)|go:"GO:0106004"("tRNA (guanine-N7)-methylation")|go:"GO:0106143"("tRNA (m7G46) methyltransferase complex")|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR028884|interpro:IPR036322|rcsb pdb:2VDU|dip:DIP-8795N|mint:Q03774 refseq:NP_010080.1|sgd:S000002360|ensemblfungi:YDL201W(gene)|ensemblfungi:YDL201W_mRNA(transcript)|go:"GO:0000049"(tRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0008176"("tRNA (guanine-N7-)-methyltransferase activity")|go:"GO:0030488"(tRNA methylation)|go:"GO:0036265"("RNA (guanine-N7)-methylation")|go:"GO:0043527"(tRNA methyltransferase complex)|go:"GO:0106004"("tRNA (guanine-N7)-methylation")|go:"GO:0106143"("tRNA (m7G46) methyltransferase complex")|interpro:IPR003358(Putative methyltransferase)|interpro:IPR025763|interpro:IPR029063|rcsb pdb:2VDU|rcsb pdb:2VDV|dip:DIP-8614N|mint:Q12009 - crc64:F1E0847F44376969 crc64:0280B9A7B02C85B7 figure legend:Fig 9|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) - 2016/09/13 2016/09/14 rogid:YRWUfQr4Jlzws67uFucIXn9XQGI559292 rogid:06ACXIJdYzcgtDlZwCmDsidozno559292 rigid:dLhujMC1adf3uCBDKwLcwHD9F6A false myc tag:n-n tandem tag:c-c - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot)