#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P09482	uniprotkb:P09484	intact:EBI-10686088|ensembl:ENSGALP00000009302	intact:EBI-10686072	psi-mi:acha4_chick(display_long)|uniprotkb:CHRNA4(gene name)|psi-mi:CHRNA4(display_short)	psi-mi:achb2_chick(display_long)|uniprotkb:CHRNB2(gene name)|psi-mi:CHRNB2(display_short)|uniprotkb:Neuronal acetylcholine receptor non-alpha-1 chain(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Anand et al. (1991)	pubmed:2040627|imex:IM-24068	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10766422|imex:IM-24068-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_990145.1|ensembl:ENSGALG00000005801(gene)|ensembl:ENSGALT00000009316(transcript)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006281"(DNA repair)|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR036719|interpro:IPR036734	interpro:IPR032932|refseq:NP_990144.1|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR018000|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043204"(perikaryon)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|interpro:IPR036719|interpro:IPR036734	-	-	-	figure legend:Fig. 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:8355(xenla-oocyte)|taxid:8355(Xenopus laevis)	-	2015/04/17	2015/04/23	rogid:6Zi4QLM752J2YldSj21kGxfeEp09031	rogid:W8PeYpMt64vzQGyNy39VgQbSd889031	rigid:A4mkfEARbeNDQz8UX/hUFPZYaPU	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P09482	uniprotkb:P09484	intact:EBI-10686088|ensembl:ENSGALP00000009302	intact:EBI-10686072	psi-mi:acha4_chick(display_long)|uniprotkb:CHRNA4(gene name)|psi-mi:CHRNA4(display_short)	psi-mi:achb2_chick(display_long)|uniprotkb:CHRNB2(gene name)|psi-mi:CHRNB2(display_short)|uniprotkb:Neuronal acetylcholine receptor non-alpha-1 chain(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Anand et al. (1991)	pubmed:2040627|imex:IM-24068	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10766435|imex:IM-24068-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_990145.1|ensembl:ENSGALG00000005801(gene)|ensembl:ENSGALT00000009316(transcript)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006281"(DNA repair)|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR036719|interpro:IPR036734	interpro:IPR032932|refseq:NP_990144.1|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR018000|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043204"(perikaryon)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|interpro:IPR036719|interpro:IPR036734	-	-	-	figure legend:Fig. 2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:8355(xenla-oocyte)|taxid:8355(Xenopus laevis)	-	2015/04/17	2015/04/23	rogid:6Zi4QLM752J2YldSj21kGxfeEp09031	rogid:W8PeYpMt64vzQGyNy39VgQbSd889031	rigid:A4mkfEARbeNDQz8UX/hUFPZYaPU	false	35s radiolabel:?-?	35s radiolabel:?-?	-	-	psi-mi:"MI:0815"(confirmation by molecular weight)	psi-mi:"MI:0815"(confirmation by molecular weight)
uniprotkb:P09482	uniprotkb:P09484	intact:EBI-10686088|ensembl:ENSGALP00000009302	intact:EBI-10686072	psi-mi:acha4_chick(display_long)|uniprotkb:CHRNA4(gene name)|psi-mi:CHRNA4(display_short)	psi-mi:achb2_chick(display_long)|uniprotkb:CHRNB2(gene name)|psi-mi:CHRNB2(display_short)|uniprotkb:Neuronal acetylcholine receptor non-alpha-1 chain(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Anand et al. (1991)	pubmed:2040627|imex:IM-24068	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10766454|imex:IM-24068-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_990145.1|ensembl:ENSGALG00000005801(gene)|ensembl:ENSGALT00000009316(transcript)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006281"(DNA repair)|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR036719|interpro:IPR036734	interpro:IPR032932|refseq:NP_990144.1|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR018000|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043204"(perikaryon)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|interpro:IPR036719|interpro:IPR036734	-	-	-	figure legend:"Fig. 4 (lane 2), Table 1"|comment:Protein complexes were isolated on a sucrose gradient and affinity purified with anti-beta2. Stoichiometry was determined by liquid scintillation counting of the [35S]methionine signature of each band normalised to the number of methionines in each protein.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:8355(xenla-oocyte)|taxid:8355(Xenopus laevis)	-	2015/04/17	2015/04/23	rogid:6Zi4QLM752J2YldSj21kGxfeEp09031	rogid:W8PeYpMt64vzQGyNy39VgQbSd889031	rigid:A4mkfEARbeNDQz8UX/hUFPZYaPU	false	35s radiolabel:?-?	35s radiolabel:?-?	2	3	psi-mi:"MI:0815"(confirmation by molecular weight)	psi-mi:"MI:0815"(confirmation by molecular weight)
uniprotkb:P02710	uniprotkb:P02718	intact:EBI-9008972	intact:EBI-8614178|intact:MINT-8146728	psi-mi:acha_tetcf(display_long)|uniprotkb:CHRNA1(gene name)|psi-mi:CHRNA1(display_short)	psi-mi:achd_tetcf(display_long)|uniprotkb:chrnd(gene name)|psi-mi:chrnd(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Anand et al. (1991)	pubmed:2040627|imex:IM-24068	taxid:7787(tetcf)|taxid:7787("Torpedo californica (Pacific electric ray)")	taxid:7787(tetcf)|taxid:7787("Torpedo californica (Pacific electric ray)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10766472|imex:IM-24068-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|rcsb pdb:1ABT|rcsb pdb:1DXZ|rcsb pdb:1IDG|rcsb pdb:1IDH|rcsb pdb:1LK1|rcsb pdb:1LXG|rcsb pdb:1LXH|rcsb pdb:1OLK|rcsb pdb:1TOR|rcsb pdb:1TOS|rcsb pdb:3MRA|interpro:IPR036719|interpro:IPR036734|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0045211"(postsynaptic membrane)|rcsb pdb:6UWZ	go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0045211"(postsynaptic membrane)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|rcsb pdb:1OLK|interpro:IPR018000|interpro:IPR006201(Neurotransmitter-gated ion-channel)|rcsb pdb:1EQ8|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|rcsb pdb:1LK1|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|rcsb pdb:1OED|interpro:IPR036719|interpro:IPR036734|rcsb pdb:6UWZ	-	-	comment:mint	figure legend:"Fig. 5 (lane 1)"|comment:Protein complexes were isolated on a sucrose gradient and affinity purified with anti-alpha1. Stoichiometry was determined by liquid scintillation counting of the [35S]methionine signature of each band normalised to the number of methionines in each protein.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:8355(xenla-oocyte)|taxid:8355(Xenopus laevis)	-	2015/04/17	2015/04/17	rogid:kS40VQzsNz5LlvUrueBI24aEy+07787	rogid:j2sFFRtuKnIRTA/DzWmCia68ShE7787	rigid:pxCMirOVxT2Gm3bI8qVRDR3cY/k	false	35s radiolabel:?-?	35s radiolabel:?-?	2	1	psi-mi:"MI:0815"(confirmation by molecular weight)	psi-mi:"MI:0815"(confirmation by molecular weight)
uniprotkb:P02710	uniprotkb:P02714	intact:EBI-9008972	intact:EBI-9008968	psi-mi:acha_tetcf(display_long)|uniprotkb:CHRNA1(gene name)|psi-mi:CHRNA1(display_short)	psi-mi:achg_tetcf(display_long)|uniprotkb:CHRNG(gene name)|psi-mi:CHRNG(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Anand et al. (1991)	pubmed:2040627|imex:IM-24068	taxid:7787(tetcf)|taxid:7787("Torpedo californica (Pacific electric ray)")	taxid:7787(tetcf)|taxid:7787("Torpedo californica (Pacific electric ray)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10766472|imex:IM-24068-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|rcsb pdb:1ABT|rcsb pdb:1DXZ|rcsb pdb:1IDG|rcsb pdb:1IDH|rcsb pdb:1LK1|rcsb pdb:1LXG|rcsb pdb:1LXH|rcsb pdb:1OLK|rcsb pdb:1TOR|rcsb pdb:1TOS|rcsb pdb:3MRA|interpro:IPR036719|interpro:IPR036734|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0045211"(postsynaptic membrane)|rcsb pdb:6UWZ	interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|rcsb pdb:1LK1|rcsb pdb:1OED|rcsb pdb:1OLK|interpro:IPR036719|interpro:IPR036734|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0045211"(postsynaptic membrane)|rcsb pdb:6UWZ	-	-	-	figure legend:"Fig. 5 (lane 1)"|comment:Protein complexes were isolated on a sucrose gradient and affinity purified with anti-alpha1. Stoichiometry was determined by liquid scintillation counting of the [35S]methionine signature of each band normalised to the number of methionines in each protein.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:8355(xenla-oocyte)|taxid:8355(Xenopus laevis)	-	2015/04/17	2015/04/17	rogid:kS40VQzsNz5LlvUrueBI24aEy+07787	rogid:5ViH5NkAFChDFcjKFETfGpDSJKM7787	rigid:pxCMirOVxT2Gm3bI8qVRDR3cY/k	false	35s radiolabel:?-?	35s radiolabel:?-?	2	1	psi-mi:"MI:0815"(confirmation by molecular weight)	psi-mi:"MI:0815"(confirmation by molecular weight)
uniprotkb:P02710	uniprotkb:P02712	intact:EBI-9008972	intact:EBI-9008960	psi-mi:acha_tetcf(display_long)|uniprotkb:CHRNA1(gene name)|psi-mi:CHRNA1(display_short)	psi-mi:achb_tetcf(display_long)|uniprotkb:CHRNB1(gene name)|psi-mi:CHRNB1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Anand et al. (1991)	pubmed:2040627|imex:IM-24068	taxid:7787(tetcf)|taxid:7787("Torpedo californica (Pacific electric ray)")	taxid:7787(tetcf)|taxid:7787("Torpedo californica (Pacific electric ray)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10766472|imex:IM-24068-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|rcsb pdb:1ABT|rcsb pdb:1DXZ|rcsb pdb:1IDG|rcsb pdb:1IDH|rcsb pdb:1LK1|rcsb pdb:1LXG|rcsb pdb:1LXH|rcsb pdb:1OLK|rcsb pdb:1TOR|rcsb pdb:1TOS|rcsb pdb:3MRA|interpro:IPR036719|interpro:IPR036734|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0045211"(postsynaptic membrane)|rcsb pdb:6UWZ	interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|rcsb pdb:1LK1|rcsb pdb:1OED|rcsb pdb:1OLK|interpro:IPR036719|interpro:IPR036734|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0045211"(postsynaptic membrane)|rcsb pdb:6UWZ	-	-	-	figure legend:"Fig. 5 (lane 1)"|comment:Protein complexes were isolated on a sucrose gradient and affinity purified with anti-alpha1. Stoichiometry was determined by liquid scintillation counting of the [35S]methionine signature of each band normalised to the number of methionines in each protein.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:8355(xenla-oocyte)|taxid:8355(Xenopus laevis)	-	2015/04/17	2015/04/17	rogid:kS40VQzsNz5LlvUrueBI24aEy+07787	rogid:UUleriIK14iT8vvpPlyHKIrG1Fc7787	rigid:pxCMirOVxT2Gm3bI8qVRDR3cY/k	false	35s radiolabel:?-?	35s radiolabel:?-?	2	1	psi-mi:"MI:0815"(confirmation by molecular weight)	psi-mi:"MI:0815"(confirmation by molecular weight)
uniprotkb:P02708	uniprotkb:P02718	intact:EBI-9008819|uniprotkb:D3DPE8|uniprotkb:B4DRV6|ensembl:ENSP00000261007	intact:EBI-8614178|intact:MINT-8146728	psi-mi:acha_human(display_long)|uniprotkb:CHRNA1(gene name)|psi-mi:CHRNA1(display_short)|uniprotkb:ACHRA(gene name synonym)|uniprotkb:CHNRA(gene name synonym)	psi-mi:achd_tetcf(display_long)|uniprotkb:chrnd(gene name)|psi-mi:chrnd(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Anand et al. (1991)	pubmed:2040627|imex:IM-24068	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:7787(tetcf)|taxid:7787("Torpedo californica (Pacific electric ray)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10766499|imex:IM-24068-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_000070.1|refseq:NP_001034612.1|interpro:IPR036719|interpro:IPR036734|rcsb pdb:1Y5P|rcsb pdb:1Y5T|rcsb pdb:1Y6C|rcsb pdb:4ZJS|rcsb pdb:5HBT|reactome:R-HSA-629594|reactome:R-HSA-629597|ensembl:ENSG00000138435(gene)|ensembl:ENST00000261007(transcript)|go:"GO:0003009"(skeletal muscle contraction)|go:"GO:0005216"(ion channel activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007274"(neuromuscular synaptic transmission)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0009986"(cell surface)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0019228"(neuronal action potential)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042166"(acetylcholine binding)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0046716"(muscle cell cellular homeostasis)|go:"GO:0048630"(skeletal muscle tissue growth)|go:"GO:0050877"(nervous system process)|go:"GO:0050881"(musculoskeletal movement)|go:"GO:0050905"(neuromuscular process)|go:"GO:0070050"(neuron cellular homeostasis)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|go:"GO:1904315"(transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000	go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0045211"(postsynaptic membrane)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|rcsb pdb:1OLK|interpro:IPR018000|interpro:IPR006201(Neurotransmitter-gated ion-channel)|rcsb pdb:1EQ8|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|rcsb pdb:1LK1|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|rcsb pdb:1OED|interpro:IPR036719|interpro:IPR036734|rcsb pdb:6UWZ	-	-	comment:mint	figure legend:"Fig. 5 (lane 2)"|comment:Protein complexes were isolated on a sucrose gradient and affinity purified with anti-alpha1. Stoichiometry was determined by liquid scintillation counting of the [35S]methionine signature of each band normalised to the number of methionines in each protein.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:8355(xenla-oocyte)|taxid:8355(Xenopus laevis)	-	2015/04/17	2015/04/17	rogid:72gC9QEvJzwEExc2m6UKXM/rKS49606	rogid:j2sFFRtuKnIRTA/DzWmCia68ShE7787	rigid:Nn9pq/V+j2sUywHW7GqXSgQ5QkM	false	35s radiolabel:?-?	35s radiolabel:?-?	2	1	psi-mi:"MI:0815"(confirmation by molecular weight)	psi-mi:"MI:0815"(confirmation by molecular weight)
uniprotkb:P02708	uniprotkb:P02714	intact:EBI-9008819|uniprotkb:D3DPE8|uniprotkb:B4DRV6|ensembl:ENSP00000261007	intact:EBI-9008968	psi-mi:acha_human(display_long)|uniprotkb:CHRNA1(gene name)|psi-mi:CHRNA1(display_short)|uniprotkb:ACHRA(gene name synonym)|uniprotkb:CHNRA(gene name synonym)	psi-mi:achg_tetcf(display_long)|uniprotkb:CHRNG(gene name)|psi-mi:CHRNG(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Anand et al. (1991)	pubmed:2040627|imex:IM-24068	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:7787(tetcf)|taxid:7787("Torpedo californica (Pacific electric ray)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10766499|imex:IM-24068-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_000070.1|refseq:NP_001034612.1|interpro:IPR036719|interpro:IPR036734|rcsb pdb:1Y5P|rcsb pdb:1Y5T|rcsb pdb:1Y6C|rcsb pdb:4ZJS|rcsb pdb:5HBT|reactome:R-HSA-629594|reactome:R-HSA-629597|ensembl:ENSG00000138435(gene)|ensembl:ENST00000261007(transcript)|go:"GO:0003009"(skeletal muscle contraction)|go:"GO:0005216"(ion channel activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007274"(neuromuscular synaptic transmission)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0009986"(cell surface)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0019228"(neuronal action potential)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042166"(acetylcholine binding)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0046716"(muscle cell cellular homeostasis)|go:"GO:0048630"(skeletal muscle tissue growth)|go:"GO:0050877"(nervous system process)|go:"GO:0050881"(musculoskeletal movement)|go:"GO:0050905"(neuromuscular process)|go:"GO:0070050"(neuron cellular homeostasis)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|go:"GO:1904315"(transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000	interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|rcsb pdb:1LK1|rcsb pdb:1OED|rcsb pdb:1OLK|interpro:IPR036719|interpro:IPR036734|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0045211"(postsynaptic membrane)|rcsb pdb:6UWZ	-	-	-	figure legend:"Fig. 5 (lane 2)"|comment:Protein complexes were isolated on a sucrose gradient and affinity purified with anti-alpha1. Stoichiometry was determined by liquid scintillation counting of the [35S]methionine signature of each band normalised to the number of methionines in each protein.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:8355(xenla-oocyte)|taxid:8355(Xenopus laevis)	-	2015/04/17	2015/04/17	rogid:72gC9QEvJzwEExc2m6UKXM/rKS49606	rogid:5ViH5NkAFChDFcjKFETfGpDSJKM7787	rigid:Nn9pq/V+j2sUywHW7GqXSgQ5QkM	false	35s radiolabel:?-?	35s radiolabel:?-?	2	1	psi-mi:"MI:0815"(confirmation by molecular weight)	psi-mi:"MI:0815"(confirmation by molecular weight)
uniprotkb:P02708	uniprotkb:P02712	intact:EBI-9008819|uniprotkb:D3DPE8|uniprotkb:B4DRV6|ensembl:ENSP00000261007	intact:EBI-9008960	psi-mi:acha_human(display_long)|uniprotkb:CHRNA1(gene name)|psi-mi:CHRNA1(display_short)|uniprotkb:ACHRA(gene name synonym)|uniprotkb:CHNRA(gene name synonym)	psi-mi:achb_tetcf(display_long)|uniprotkb:CHRNB1(gene name)|psi-mi:CHRNB1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Anand et al. (1991)	pubmed:2040627|imex:IM-24068	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:7787(tetcf)|taxid:7787("Torpedo californica (Pacific electric ray)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10766499|imex:IM-24068-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_000070.1|refseq:NP_001034612.1|interpro:IPR036719|interpro:IPR036734|rcsb pdb:1Y5P|rcsb pdb:1Y5T|rcsb pdb:1Y6C|rcsb pdb:4ZJS|rcsb pdb:5HBT|reactome:R-HSA-629594|reactome:R-HSA-629597|ensembl:ENSG00000138435(gene)|ensembl:ENST00000261007(transcript)|go:"GO:0003009"(skeletal muscle contraction)|go:"GO:0005216"(ion channel activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007274"(neuromuscular synaptic transmission)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0009986"(cell surface)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0019228"(neuronal action potential)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042166"(acetylcholine binding)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0046716"(muscle cell cellular homeostasis)|go:"GO:0048630"(skeletal muscle tissue growth)|go:"GO:0050877"(nervous system process)|go:"GO:0050881"(musculoskeletal movement)|go:"GO:0050905"(neuromuscular process)|go:"GO:0070050"(neuron cellular homeostasis)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|go:"GO:1904315"(transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000	interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|rcsb pdb:1LK1|rcsb pdb:1OED|rcsb pdb:1OLK|interpro:IPR036719|interpro:IPR036734|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0045211"(postsynaptic membrane)|rcsb pdb:6UWZ	-	-	-	figure legend:"Fig. 5 (lane 2)"|comment:Protein complexes were isolated on a sucrose gradient and affinity purified with anti-alpha1. Stoichiometry was determined by liquid scintillation counting of the [35S]methionine signature of each band normalised to the number of methionines in each protein.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:8355(xenla-oocyte)|taxid:8355(Xenopus laevis)	-	2015/04/17	2015/04/17	rogid:72gC9QEvJzwEExc2m6UKXM/rKS49606	rogid:UUleriIK14iT8vvpPlyHKIrG1Fc7787	rigid:Nn9pq/V+j2sUywHW7GqXSgQ5QkM	false	35s radiolabel:?-?	35s radiolabel:?-?	2	1	psi-mi:"MI:0815"(confirmation by molecular weight)	psi-mi:"MI:0815"(confirmation by molecular weight)
uniprotkb:P09482	uniprotkb:P09484	intact:EBI-10686088|ensembl:ENSGALP00000009302	intact:EBI-10686072	psi-mi:acha4_chick(display_long)|uniprotkb:CHRNA4(gene name)|psi-mi:CHRNA4(display_short)	psi-mi:achb2_chick(display_long)|uniprotkb:CHRNB2(gene name)|psi-mi:CHRNB2(display_short)|uniprotkb:Neuronal acetylcholine receptor non-alpha-1 chain(gene name synonym)	psi-mi:"MI:0096"(pull down)	Anand et al. (1991)	pubmed:2040627|imex:IM-24068	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10766462|imex:IM-24068-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_990145.1|ensembl:ENSGALG00000005801(gene)|ensembl:ENSGALT00000009316(transcript)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006281"(DNA repair)|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR036719|interpro:IPR036734	interpro:IPR032932|refseq:NP_990144.1|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR018000|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043204"(perikaryon)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|interpro:IPR036719|interpro:IPR036734	-	-	-	figure legend:"Fig. 4 (lane 1), Table 1"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:8355(xenla-oocyte)|taxid:8355(Xenopus laevis)	-	2015/04/17	2015/04/23	rogid:6Zi4QLM752J2YldSj21kGxfeEp09031	rogid:W8PeYpMt64vzQGyNy39VgQbSd889031	rigid:A4mkfEARbeNDQz8UX/hUFPZYaPU	false	35s radiolabel:?-?	35s radiolabel:?-?	2	3	psi-mi:"MI:0815"(confirmation by molecular weight)	psi-mi:"MI:0815"(confirmation by molecular weight)