#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P74182	uniprotkb:P74182	intact:EBI-9691722|ensemblbacteria:BAA18271	intact:EBI-9691722|ensemblbacteria:BAA18271	psi-mi:p74182_syny3(display_long)|uniprotkb:slr1270(locus name)	psi-mi:p74182_syny3(display_long)|uniprotkb:slr1270(locus name)	psi-mi:"MI:0071"(molecular sieving)	Agarwal et al. (2014)	pubmed:25218435|imex:IM-23170	taxid:1111708(syny3)|taxid:1111708("Synechocystis sp. (strain PCC 6803 / Kazusa)")	taxid:1111708(syny3)|taxid:1111708("Synechocystis sp. (strain PCC 6803 / Kazusa)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9691719|imex:IM-23170-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR003423(Outer membrane efflux protein)|ensemblbacteria:BAA18271(gene)|ensemblbacteria:BAA18271(transcript)|go:"GO:0015562"(efflux transmembrane transporter activity)|go:"GO:0030288"(outer membrane-bounded periplasmic space)|go:"GO:0042802"(identical protein binding)	interpro:IPR003423(Outer membrane efflux protein)|ensemblbacteria:BAA18271(gene)|ensemblbacteria:BAA18271(transcript)|go:"GO:0015562"(efflux transmembrane transporter activity)|go:"GO:0030288"(outer membrane-bounded periplasmic space)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:f3|comment:"Slr1270  fractions purified by Ni-NTA chromatography were pooled and refolded through stepwise  dialysis in the presence of 0.1% UDM detergent. The refolded protein, loaded on a Superdex-  200 size exclusion FPLC column, eluted in two peaks at 8.3 mL and 10.7 mL (Fig. 3A), similar  to the elution pattern of native E. coli TolC (elution curve in red), supporting correct refolding of  the Slr1270. The peak at 8.3 mL corresponds to protein aggregates with Mr > 400 kDa (Fig. 3A).  The peak at 10.7 mL contained trimeric Slr1270 protein (Fig. 3A) as it displayed a single band  on clear-native PAGE corresponding to a trimer similar to native E. coli TolC (Fig. 3B, lanes 2  and 5). The small difference in migration is consistent with the difference in protein size  (Slr1270 monomer ~ 55 kDa; E. coli TolC monomer ~ 50 kDa). However, the Slr1270 protein  showed a concentration dependent aggregation (Fig. 3B, lanes 3-4)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/08/20	2014/10/16	rogid:RS2c5Iby/6asB/KwKbiVtmOPTOg1111708	rogid:RS2c5Iby/6asB/KwKbiVtmOPTOg1111708	intact-crc:1161E0D1A6C77C64|rigid:OwepLonf6UZBpHSQ+We/kvmnDIE	false	his tag:?-?	his tag:?-?	-	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P74182	uniprotkb:P74182	intact:EBI-9691722|ensemblbacteria:BAA18271	intact:EBI-9691722|ensemblbacteria:BAA18271	psi-mi:p74182_syny3(display_long)|uniprotkb:slr1270(locus name)	psi-mi:p74182_syny3(display_long)|uniprotkb:slr1270(locus name)	psi-mi:"MI:0071"(molecular sieving)	Agarwal et al. (2014)	pubmed:25218435|imex:IM-23170	taxid:1111708(syny3)|taxid:1111708("Synechocystis sp. (strain PCC 6803 / Kazusa)")	taxid:1111708(syny3)|taxid:1111708("Synechocystis sp. (strain PCC 6803 / Kazusa)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9691758|imex:IM-23170-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR003423(Outer membrane efflux protein)|ensemblbacteria:BAA18271(gene)|ensemblbacteria:BAA18271(transcript)|go:"GO:0015562"(efflux transmembrane transporter activity)|go:"GO:0030288"(outer membrane-bounded periplasmic space)|go:"GO:0042802"(identical protein binding)	interpro:IPR003423(Outer membrane efflux protein)|ensemblbacteria:BAA18271(gene)|ensemblbacteria:BAA18271(transcript)|go:"GO:0015562"(efflux transmembrane transporter activity)|go:"GO:0030288"(outer membrane-bounded periplasmic space)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:f3|comment:"Slr1270  fractions purified by Ni-NTA chromatography were pooled and refolded through stepwise  dialysis in the presence of 0.1% UDM detergent. The refolded protein, loaded on a Superdex-  200 size exclusion FPLC column, eluted in two peaks at 8.3 mL and 10.7 mL (Fig. 3A), similar  to the elution pattern of native E. coli TolC (elution curve in red), supporting correct refolding of  the Slr1270. The peak at 8.3 mL corresponds to protein aggregates with Mr > 400 kDa (Fig. 3A).  The peak at 10.7 mL contained trimeric Slr1270 protein (Fig. 3A) as it displayed a single band  on clear-native PAGE corresponding to a trimer similar to native E. coli TolC (Fig. 3B, lanes 2  and 5). The small difference in migration is consistent with the difference in protein size  (Slr1270 monomer ~ 55 kDa; E. coli TolC monomer ~ 50 kDa). However, the Slr1270 protein  showed a concentration dependent aggregation (Fig. 3B, lanes 3-4)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/08/20	2014/10/16	rogid:RS2c5Iby/6asB/KwKbiVtmOPTOg1111708	rogid:RS2c5Iby/6asB/KwKbiVtmOPTOg1111708	intact-crc:F6695865B29299F0|rigid:1cWRxO2bfz6Fsr05AW1aiYwzV/g	false	his tag:?-?	his tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P02930	uniprotkb:P02930	intact:EBI-875614|uniprotkb:Q2M9G5|ensemblbacteria:AAC76071|ensemblbacteria:BAE77091	intact:EBI-875614|uniprotkb:Q2M9G5|ensemblbacteria:AAC76071|ensemblbacteria:BAE77091	psi-mi:tolc_ecoli(display_long)|uniprotkb:tolC(gene name)|psi-mi:tolC(display_short)|uniprotkb:mtcB(gene name synonym)|uniprotkb:mukA(gene name synonym)|uniprotkb:refI(gene name synonym)|uniprotkb:Multidrug efflux pump subunit TolC(gene name synonym)|uniprotkb:Outer membrane factor TolC(gene name synonym)|uniprotkb:colE1-i(gene name synonym)|uniprotkb:toc(gene name synonym)|uniprotkb:weeA(gene name synonym)|uniprotkb:JW5503(locus name)|uniprotkb:b3035(locus name)	psi-mi:tolc_ecoli(display_long)|uniprotkb:tolC(gene name)|psi-mi:tolC(display_short)|uniprotkb:mtcB(gene name synonym)|uniprotkb:mukA(gene name synonym)|uniprotkb:refI(gene name synonym)|uniprotkb:Multidrug efflux pump subunit TolC(gene name synonym)|uniprotkb:Outer membrane factor TolC(gene name synonym)|uniprotkb:colE1-i(gene name synonym)|uniprotkb:toc(gene name synonym)|uniprotkb:weeA(gene name synonym)|uniprotkb:JW5503(locus name)|uniprotkb:b3035(locus name)	psi-mi:"MI:0071"(molecular sieving)	Agarwal et al. (2014)	pubmed:25218435|imex:IM-23170	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9691781|imex:IM-23170-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417507.2|refseq:WP_000735278.1|dip:DIP-11007N|ensemblbacteria:AAC76071(transcript)|ensemblbacteria:b3035(gene)|ensemblbacteria:BAE77091(gene)|ensemblbacteria:BAE77091(transcript)|go:"GO:0005216"(ion channel activity)|go:"GO:0009279"(cell outer membrane)|go:"GO:0009636"(response to toxic substance)|go:"GO:0014070"(response to organic cyclic compound)|go:"GO:0015125"(bile acid transmembrane transporter activity)|go:"GO:0015288"(porin activity)|go:"GO:0015562"(efflux transmembrane transporter activity)|go:"GO:0015721"(bile acid and bile salt transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030288"(outer membrane-bounded periplasmic space)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042493"|go:"GO:0042802"(identical protein binding)|go:"GO:0042930"(enterobactin transport)|go:"GO:0045203"(integral component of cell outer membrane)|go:"GO:0046677"(response to antibiotic)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:1990196"(MacAB-TolC complex)|go:"GO:1990281"(efflux pump complex)|interpro:IPR003423(Outer membrane efflux protein)|interpro:IPR010130(Type I secretion outer membrane protein, TolC)|rcsb pdb:1EK9|rcsb pdb:1TQQ|rcsb pdb:2VDD|rcsb pdb:2VDE|rcsb pdb:2WMZ|rcsb pdb:2XMN|rcsb pdb:5NG5|rcsb pdb:5NIK|rcsb pdb:5NIL|rcsb pdb:5O66|rcsb pdb:5V5S|rcsb pdb:6WXH|rcsb pdb:6WXI	refseq:NP_417507.2|refseq:WP_000735278.1|dip:DIP-11007N|ensemblbacteria:AAC76071(transcript)|ensemblbacteria:b3035(gene)|ensemblbacteria:BAE77091(gene)|ensemblbacteria:BAE77091(transcript)|go:"GO:0005216"(ion channel activity)|go:"GO:0009279"(cell outer membrane)|go:"GO:0009636"(response to toxic substance)|go:"GO:0014070"(response to organic cyclic compound)|go:"GO:0015125"(bile acid transmembrane transporter activity)|go:"GO:0015288"(porin activity)|go:"GO:0015562"(efflux transmembrane transporter activity)|go:"GO:0015721"(bile acid and bile salt transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030288"(outer membrane-bounded periplasmic space)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042493"|go:"GO:0042802"(identical protein binding)|go:"GO:0042930"(enterobactin transport)|go:"GO:0045203"(integral component of cell outer membrane)|go:"GO:0046677"(response to antibiotic)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:1990196"(MacAB-TolC complex)|go:"GO:1990281"(efflux pump complex)|interpro:IPR003423(Outer membrane efflux protein)|interpro:IPR010130(Type I secretion outer membrane protein, TolC)|rcsb pdb:1EK9|rcsb pdb:1TQQ|rcsb pdb:2VDD|rcsb pdb:2VDE|rcsb pdb:2WMZ|rcsb pdb:2XMN|rcsb pdb:5NG5|rcsb pdb:5NIK|rcsb pdb:5NIL|rcsb pdb:5O66|rcsb pdb:5V5S|rcsb pdb:6WXH|rcsb pdb:6WXI	-	-	-	figure legend:f3a f3c|comment:"Slr1270  fractions purified by Ni-NTA chromatography were pooled and refolded through stepwise  dialysis in the presence of 0.1% UDM detergent. The refolded protein, loaded on a Superdex-  200 size exclusion FPLC column, eluted in two peaks at 8.3 mL and 10.7 mL (Fig. 3A), similar  to the elution pattern of native E. coli TolC (elution curve in red), supporting correct refolding of  the Slr1270. The peak at 8.3 mL corresponds to protein aggregates with Mr > 400 kDa (Fig. 3A).  The peak at 10.7 mL contained trimeric Slr1270 protein (Fig. 3A) as it displayed a single band  on clear-native PAGE corresponding to a trimer similar to native E. coli TolC (Fig. 3B, lanes 2  and 5). The small difference in migration is consistent with the difference in protein size  (Slr1270 monomer ~ 55 kDa; E. coli TolC monomer ~ 50 kDa). However, the Slr1270 protein  showed a concentration dependent aggregation (Fig. 3B, lanes 3-4)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/08/20	2014/10/16	rogid:67LQDsYiAt6tDwigzlhM3Pp5+JA83333	rogid:67LQDsYiAt6tDwigzlhM3Pp5+JA83333	intact-crc:9120BF27295C0E6A|rigid:jYYvtdyJteICiGPMzsrlAL9DGbc	false	-	-	3	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)