#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P43026	uniprotkb:P43026	intact:EBI-8571476|ensembl:ENSP00000363489|ensembl:ENSP00000363492|uniprotkb:Q96SB1|intact:MINT-7264433|uniprotkb:E1P5Q2	intact:EBI-8571476|ensembl:ENSP00000363489|ensembl:ENSP00000363492|uniprotkb:Q96SB1|intact:MINT-7264433|uniprotkb:E1P5Q2	psi-mi:gdf5_human(display_long)|uniprotkb:GDF5(gene name)|psi-mi:GDF5(display_short)|uniprotkb:CDMP1(gene name synonym)|uniprotkb:Cartilage-derived morphogenetic protein 1(gene name synonym)|uniprotkb:Radotermin(gene name synonym)|uniprotkb:BMP14(gene name synonym)|uniprotkb:Bone morphogenetic protein 14(gene name synonym)|uniprotkb:Lipopolysaccharide-associated protein 4(gene name synonym)	psi-mi:gdf5_human(display_long)|uniprotkb:GDF5(gene name)|psi-mi:GDF5(display_short)|uniprotkb:CDMP1(gene name synonym)|uniprotkb:Cartilage-derived morphogenetic protein 1(gene name synonym)|uniprotkb:Radotermin(gene name synonym)|uniprotkb:BMP14(gene name synonym)|uniprotkb:Bone morphogenetic protein 14(gene name synonym)|uniprotkb:Lipopolysaccharide-associated protein 4(gene name synonym)	psi-mi:"MI:0107"(surface plasmon resonance)	Thieme et al. (2014)	imex:IM-23073|pubmed:25174448	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9693712|imex:IM-23073-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001306067.1|ensembl:ENSG00000125965(gene)|ensembl:ENST00000374369(transcript)|ensembl:ENST00000374372(transcript)|go:"GO:0005125"(cytokine activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0007179"(transforming growth factor beta receptor signaling pathway)|go:"GO:0007267"(cell-cell signaling)|go:"GO:0008083"(growth factor activity)|go:"GO:0009612"(response to mechanical stimulus)|go:"GO:0010862"(positive regulation of pathway-restricted SMAD protein phosphorylation)|go:"GO:0030326"(embryonic limb morphogenesis)|go:"GO:0030509"(BMP signaling pathway)|go:"GO:0030513"(positive regulation of BMP signaling pathway)|go:"GO:0032331"(negative regulation of chondrocyte differentiation)|go:"GO:0032332"(positive regulation of chondrocyte differentiation)|go:"GO:0035136"(forelimb morphogenesis)|go:"GO:0035137"(hindlimb morphogenesis)|go:"GO:0036122"(BMP binding)|go:"GO:0040014"(regulation of multicellular organism growth)|go:"GO:0042802"(identical protein binding)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0043932"(ossification involved in bone remodeling)|go:"GO:0045666"(positive regulation of neuron differentiation)|go:"GO:0050680"(negative regulation of epithelial cell proliferation)|go:"GO:0060390"(regulation of SMAD protein signal transduction)|go:"GO:0060395"(SMAD protein signal transduction)|interpro:IPR001839(Transforming growth factor beta)|interpro:IPR015615(Transforming growth factor-beta-related)|interpro:IPR017948|interpro:IPR029034|mint:P43026|rcsb pdb:1WAQ|rcsb pdb:2BHK|rcsb pdb:3EVS|rcsb pdb:3QB4|rcsb pdb:5HK5|rcsb pdb:6Z3G|rcsb pdb:6Z3H|go:"GO:0060591"(chondroblast differentiation)|go:"GO:2001054"(negative regulation of mesenchymal cell apoptotic process)|interpro:IPR001111("Transforming growth factor beta (TGFb), N-terminal")|rcsb pdb:6Z3J|rcsb pdb:6Z3L|rcsb pdb:6Z3M|reactome:R-HSA-2129379|refseq:NP_000548.2|dip:DIP-5823N|refseq:XP_011527377.1	refseq:NP_001306067.1|ensembl:ENSG00000125965(gene)|ensembl:ENST00000374369(transcript)|ensembl:ENST00000374372(transcript)|go:"GO:0005125"(cytokine activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0007179"(transforming growth factor beta receptor signaling pathway)|go:"GO:0007267"(cell-cell signaling)|go:"GO:0008083"(growth factor activity)|go:"GO:0009612"(response to mechanical stimulus)|go:"GO:0010862"(positive regulation of pathway-restricted SMAD protein phosphorylation)|go:"GO:0030326"(embryonic limb morphogenesis)|go:"GO:0030509"(BMP signaling pathway)|go:"GO:0030513"(positive regulation of BMP signaling pathway)|go:"GO:0032331"(negative regulation of chondrocyte differentiation)|go:"GO:0032332"(positive regulation of chondrocyte differentiation)|go:"GO:0035136"(forelimb morphogenesis)|go:"GO:0035137"(hindlimb morphogenesis)|go:"GO:0036122"(BMP binding)|go:"GO:0040014"(regulation of multicellular organism growth)|go:"GO:0042802"(identical protein binding)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0043932"(ossification involved in bone remodeling)|go:"GO:0045666"(positive regulation of neuron differentiation)|go:"GO:0050680"(negative regulation of epithelial cell proliferation)|go:"GO:0060390"(regulation of SMAD protein signal transduction)|go:"GO:0060395"(SMAD protein signal transduction)|interpro:IPR001839(Transforming growth factor beta)|interpro:IPR015615(Transforming growth factor-beta-related)|interpro:IPR017948|interpro:IPR029034|mint:P43026|rcsb pdb:1WAQ|rcsb pdb:2BHK|rcsb pdb:3EVS|rcsb pdb:3QB4|rcsb pdb:5HK5|rcsb pdb:6Z3G|rcsb pdb:6Z3H|go:"GO:0060591"(chondroblast differentiation)|go:"GO:2001054"(negative regulation of mesenchymal cell apoptotic process)|interpro:IPR001111("Transforming growth factor beta (TGFb), N-terminal")|rcsb pdb:6Z3J|rcsb pdb:6Z3L|rcsb pdb:6Z3M|reactome:R-HSA-2129379|refseq:NP_000548.2|dip:DIP-5823N|refseq:XP_011527377.1	-	comment:mint	comment:mint	figure legend:f7a|comment:"part. In order to address this assumption via an independent experimental approach,  the interaction of the proregion with the mature part was analyzed in SPR experiments.  Mature GDF5 was immobilized and the proregion used as a soluble analyte. Association of  the wild type proregion with mature GDF5 deviated from a 1 : 1 interaction. The curve shape  was interpreted according to two binding reactions and one association constant with KD =  15.1 nM and the second one with KD = 911 nM. It is possible that the low affinity interaction  reflects the association of the proregion with the mature part that has already bound one  molecule of proregion. Association of GDF5 with the T201P proregion occurred at clearly  reduced affinities of KD = 304 nM and KD = 7.2 μM."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:15.1x10^-9(molar)|kd:911.0x10^-9(molar)	2014/08/25	2014/10/16	rogid:1+H+MmShXRpzq5qIDid7rv8ApMk9606	rogid:1+H+MmShXRpzq5qIDid7rv8ApMk9606	intact-crc:CE23C9C6F12795D5|rigid:Y4Czg+i2ftOxJbSW1VwxyFTjGEk	false	binding-associated region:28-381	binding-associated region:382-501	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P43026	uniprotkb:P43026	intact:EBI-8571476|ensembl:ENSP00000363489|ensembl:ENSP00000363492|uniprotkb:Q96SB1|intact:MINT-7264433|uniprotkb:E1P5Q2	intact:EBI-8571476|ensembl:ENSP00000363489|ensembl:ENSP00000363492|uniprotkb:Q96SB1|intact:MINT-7264433|uniprotkb:E1P5Q2	psi-mi:gdf5_human(display_long)|uniprotkb:GDF5(gene name)|psi-mi:GDF5(display_short)|uniprotkb:CDMP1(gene name synonym)|uniprotkb:Cartilage-derived morphogenetic protein 1(gene name synonym)|uniprotkb:Radotermin(gene name synonym)|uniprotkb:BMP14(gene name synonym)|uniprotkb:Bone morphogenetic protein 14(gene name synonym)|uniprotkb:Lipopolysaccharide-associated protein 4(gene name synonym)	psi-mi:gdf5_human(display_long)|uniprotkb:GDF5(gene name)|psi-mi:GDF5(display_short)|uniprotkb:CDMP1(gene name synonym)|uniprotkb:Cartilage-derived morphogenetic protein 1(gene name synonym)|uniprotkb:Radotermin(gene name synonym)|uniprotkb:BMP14(gene name synonym)|uniprotkb:Bone morphogenetic protein 14(gene name synonym)|uniprotkb:Lipopolysaccharide-associated protein 4(gene name synonym)	psi-mi:"MI:0107"(surface plasmon resonance)	Thieme et al. (2014)	imex:IM-23073|pubmed:25174448	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9693723|imex:IM-23073-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001306067.1|ensembl:ENSG00000125965(gene)|ensembl:ENST00000374369(transcript)|ensembl:ENST00000374372(transcript)|go:"GO:0005125"(cytokine activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0007179"(transforming growth factor beta receptor signaling pathway)|go:"GO:0007267"(cell-cell signaling)|go:"GO:0008083"(growth factor activity)|go:"GO:0009612"(response to mechanical stimulus)|go:"GO:0010862"(positive regulation of pathway-restricted SMAD protein phosphorylation)|go:"GO:0030326"(embryonic limb morphogenesis)|go:"GO:0030509"(BMP signaling pathway)|go:"GO:0030513"(positive regulation of BMP signaling pathway)|go:"GO:0032331"(negative regulation of chondrocyte differentiation)|go:"GO:0032332"(positive regulation of chondrocyte differentiation)|go:"GO:0035136"(forelimb morphogenesis)|go:"GO:0035137"(hindlimb morphogenesis)|go:"GO:0036122"(BMP binding)|go:"GO:0040014"(regulation of multicellular organism growth)|go:"GO:0042802"(identical protein binding)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0043932"(ossification involved in bone remodeling)|go:"GO:0045666"(positive regulation of neuron differentiation)|go:"GO:0050680"(negative regulation of epithelial cell proliferation)|go:"GO:0060390"(regulation of SMAD protein signal transduction)|go:"GO:0060395"(SMAD protein signal transduction)|interpro:IPR001839(Transforming growth factor beta)|interpro:IPR015615(Transforming growth factor-beta-related)|interpro:IPR017948|interpro:IPR029034|mint:P43026|rcsb pdb:1WAQ|rcsb pdb:2BHK|rcsb pdb:3EVS|rcsb pdb:3QB4|rcsb pdb:5HK5|rcsb pdb:6Z3G|rcsb pdb:6Z3H|go:"GO:0060591"(chondroblast differentiation)|go:"GO:2001054"(negative regulation of mesenchymal cell apoptotic process)|interpro:IPR001111("Transforming growth factor beta (TGFb), N-terminal")|rcsb pdb:6Z3J|rcsb pdb:6Z3L|rcsb pdb:6Z3M|reactome:R-HSA-2129379|refseq:NP_000548.2|dip:DIP-5823N|refseq:XP_011527377.1	refseq:NP_001306067.1|ensembl:ENSG00000125965(gene)|ensembl:ENST00000374369(transcript)|ensembl:ENST00000374372(transcript)|go:"GO:0005125"(cytokine activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0007179"(transforming growth factor beta receptor signaling pathway)|go:"GO:0007267"(cell-cell signaling)|go:"GO:0008083"(growth factor activity)|go:"GO:0009612"(response to mechanical stimulus)|go:"GO:0010862"(positive regulation of pathway-restricted SMAD protein phosphorylation)|go:"GO:0030326"(embryonic limb morphogenesis)|go:"GO:0030509"(BMP signaling pathway)|go:"GO:0030513"(positive regulation of BMP signaling pathway)|go:"GO:0032331"(negative regulation of chondrocyte differentiation)|go:"GO:0032332"(positive regulation of chondrocyte differentiation)|go:"GO:0035136"(forelimb morphogenesis)|go:"GO:0035137"(hindlimb morphogenesis)|go:"GO:0036122"(BMP binding)|go:"GO:0040014"(regulation of multicellular organism growth)|go:"GO:0042802"(identical protein binding)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0043932"(ossification involved in bone remodeling)|go:"GO:0045666"(positive regulation of neuron differentiation)|go:"GO:0050680"(negative regulation of epithelial cell proliferation)|go:"GO:0060390"(regulation of SMAD protein signal transduction)|go:"GO:0060395"(SMAD protein signal transduction)|interpro:IPR001839(Transforming growth factor beta)|interpro:IPR015615(Transforming growth factor-beta-related)|interpro:IPR017948|interpro:IPR029034|mint:P43026|rcsb pdb:1WAQ|rcsb pdb:2BHK|rcsb pdb:3EVS|rcsb pdb:3QB4|rcsb pdb:5HK5|rcsb pdb:6Z3G|rcsb pdb:6Z3H|go:"GO:0060591"(chondroblast differentiation)|go:"GO:2001054"(negative regulation of mesenchymal cell apoptotic process)|interpro:IPR001111("Transforming growth factor beta (TGFb), N-terminal")|rcsb pdb:6Z3J|rcsb pdb:6Z3L|rcsb pdb:6Z3M|reactome:R-HSA-2129379|refseq:NP_000548.2|dip:DIP-5823N|refseq:XP_011527377.1	-	comment:mint	comment:mint	figure legend:f7b|comment:"part. In order to address this assumption via an independent experimental approach,  the interaction of the proregion with the mature part was analyzed in SPR experiments.  Mature GDF5 was immobilized and the proregion used as a soluble analyte. Association of  the wild type proregion with mature GDF5 deviated from a 1 : 1 interaction. The curve shape  was interpreted according to two binding reactions and one association constant with KD =  15.1 nM and the second one with KD = 911 nM. It is possible that the low affinity interaction  reflects the association of the proregion with the mature part that has already bound one  molecule of proregion. Association of GDF5 with the T201P proregion occurred at clearly  reduced affinities of KD = 304 nM and KD = 7.2 μM."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:304.0x10^-9(molar)|kd:7.2x10^-6(molar)	2014/08/25	2014/10/16	rogid:1+H+MmShXRpzq5qIDid7rv8ApMk9606	rogid:1+H+MmShXRpzq5qIDid7rv8ApMk9606	intact-crc:1B6279B61D24BCD4|rigid:Y4Czg+i2ftOxJbSW1VwxyFTjGEk	false	binding-associated region:382-501	mutation decreasing interaction:201-201|binding-associated region:28-381	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)