#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P06492	uniprotkb:Q7XYY2	intact:EBI-7489933|uniprotkb:Q09I86|intact:MINT-275736|uniprotkb:B9VQH6	intact:EBI-1386292|uniprotkb:F4ICJ2|uniprotkb:Q9C6M3|ensemblplants:AT1G25540.1	psi-mi:vp16_hhv11(display_long)|uniprotkb:Vmw65(gene name synonym)|uniprotkb:ICP25(gene name synonym)|uniprotkb:Alpha-TIF(gene name synonym)|uniprotkb:Alpha trans-inducing protein(gene name synonym)|uniprotkb:UL48(orf name)	psi-mi:med25_arath(display_long)|uniprotkb:MED25(gene name)|psi-mi:MED25(display_short)|uniprotkb:MED25_1(gene name synonym)|uniprotkb:PFT1(gene name synonym)|uniprotkb:Phytochrome and flowering time 1 protein(gene name synonym)|uniprotkb:Phytochrome and flowering time regulatory protein 1(gene name synonym)|uniprotkb:F2J7.4(orf name)|uniprotkb:At1g25540(locus name)	psi-mi:"MI:0809"(bimolecular fluorescence complementation)	Fujiwara et al. (2014)	pubmed:25150167|imex:IM-23059	taxid:10299(hhv11)|taxid:10299("Human herpesvirus 1 (strain 17) (HHV-1)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9685262|imex:IM-23059-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:YP_009137123.1|mint:P06492|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0001046"(core promoter sequence-specific DNA binding)|go:"GO:0003677"(DNA binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0019033"(viral tegument)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0039660"(structural constituent of virion)|go:"GO:0039695"(DNA-templated viral transcription)|go:"GO:0042025"(host cell nucleus)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046782"(regulation of viral transcription)|go:"GO:0046809"(replication compartment)|go:"GO:0051701"(biological process involved in interaction with host)|go:"GO:0065003"(protein-containing complex assembly)|go:"GO:0140297"(DNA-binding transcription factor binding)|interpro:IPR003174("Alpha trans-inducing protein (Alpha-TIF)")|interpro:IPR021051|interpro:IPR036538|rcsb pdb:16VP|rcsb pdb:2PHE|rcsb pdb:2PHG|dip:DIP-41776N	refseq:NP_173925.3|refseq:NP_001077596.1|dip:DIP-39923N|ensemblplants:AT1G25540(gene)|ensemblplants:AT1G25540.1(transcript)|go:"GO:0003677"(DNA binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005634"(nucleus)|go:"GO:0005667"(transcription regulator complex)|go:"GO:0009585"(red, far-red light phototransduction)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009908"(flower development)|go:"GO:0009909"(regulation of flower development)|go:"GO:0009911"(positive regulation of flower development)|go:"GO:0010091"(trichome branching)|go:"GO:0010114"(response to red light)|go:"GO:0010218"(response to far red light)|go:"GO:0016592"(mediator complex)|go:"GO:0031349"(positive regulation of defense response)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050832"(defense response to fungus)|go:"GO:1905499"(trichome papilla formation)|interpro:IPR021419	-	comment:mint|function:"Responsible for transcriptional activation of immediate early promoters (alpha genes). Activation requires the formation of a heteromeric complex with the host cell factor. These two proteins then assemble with the octamer motif-binding protein Oct-1 on the cis-acting target sequence: 5'-TAATGARAT-3'"	-	figure legend:f4b|comment:"To test whether plant Med25 also interacts with VP16 in vivo, we performed  immunoprecipitation assays using the N. benthamiana system to co-express the  tagged Arabidopsis proteins (Figure 4A). FLAG-tagged Med25 (FLAG-Med25) was  co-expressed with GFP-FLC, GFP-FLC-VP16, GFP-VP16, or GFP, and the protein  complex including the GFP-tagged proteins was immunoprecipitated with an α-GFP  antibody. FLAG-Med25 was co-immunoprecipitated with the VP16-fused proteins,  GFP-FLC-VP16 and GFP-VP16, but not with GFP or GFP-FLC without VP16 fusion,  indicating that VP16 directly or indirectly interacts with Arabidopsis Med25 in vivo."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4100(nicbe)|taxid:4100(Nicotiana benthamiana)	-	2014/08/05	2014/10/16	rogid:8rAT+y7LSTotMP4XcD+eSyMpff010299	rogid:QxVH3bP188+lGliiHSlp1FrdTy43702	intact-crc:061715D965696E22|rigid:OPJfRItpqEL5GD4IjUHqViLAiOg	false	binding-associated region:413-490|yellow fluorescent protein tag:n-n	yellow fluorescent protein tag:c-c	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)
uniprotkb:P06492	uniprotkb:Q7XYY2	intact:EBI-7489933|uniprotkb:Q09I86|intact:MINT-275736|uniprotkb:B9VQH6	intact:EBI-1386292|uniprotkb:F4ICJ2|uniprotkb:Q9C6M3|ensemblplants:AT1G25540.1	psi-mi:vp16_hhv11(display_long)|uniprotkb:Vmw65(gene name synonym)|uniprotkb:ICP25(gene name synonym)|uniprotkb:Alpha-TIF(gene name synonym)|uniprotkb:Alpha trans-inducing protein(gene name synonym)|uniprotkb:UL48(orf name)	psi-mi:med25_arath(display_long)|uniprotkb:MED25(gene name)|psi-mi:MED25(display_short)|uniprotkb:MED25_1(gene name synonym)|uniprotkb:PFT1(gene name synonym)|uniprotkb:Phytochrome and flowering time 1 protein(gene name synonym)|uniprotkb:Phytochrome and flowering time regulatory protein 1(gene name synonym)|uniprotkb:F2J7.4(orf name)|uniprotkb:At1g25540(locus name)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Fujiwara et al. (2014)	pubmed:25150167|imex:IM-23059	taxid:10299(hhv11)|taxid:10299("Human herpesvirus 1 (strain 17) (HHV-1)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9685240|imex:IM-23059-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:YP_009137123.1|mint:P06492|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0001046"(core promoter sequence-specific DNA binding)|go:"GO:0003677"(DNA binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0019033"(viral tegument)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0039660"(structural constituent of virion)|go:"GO:0039695"(DNA-templated viral transcription)|go:"GO:0042025"(host cell nucleus)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046782"(regulation of viral transcription)|go:"GO:0046809"(replication compartment)|go:"GO:0051701"(biological process involved in interaction with host)|go:"GO:0065003"(protein-containing complex assembly)|go:"GO:0140297"(DNA-binding transcription factor binding)|interpro:IPR003174("Alpha trans-inducing protein (Alpha-TIF)")|interpro:IPR021051|interpro:IPR036538|rcsb pdb:16VP|rcsb pdb:2PHE|rcsb pdb:2PHG|dip:DIP-41776N	refseq:NP_173925.3|refseq:NP_001077596.1|dip:DIP-39923N|ensemblplants:AT1G25540(gene)|ensemblplants:AT1G25540.1(transcript)|go:"GO:0003677"(DNA binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005634"(nucleus)|go:"GO:0005667"(transcription regulator complex)|go:"GO:0009585"(red, far-red light phototransduction)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009908"(flower development)|go:"GO:0009909"(regulation of flower development)|go:"GO:0009911"(positive regulation of flower development)|go:"GO:0010091"(trichome branching)|go:"GO:0010114"(response to red light)|go:"GO:0010218"(response to far red light)|go:"GO:0016592"(mediator complex)|go:"GO:0031349"(positive regulation of defense response)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050832"(defense response to fungus)|go:"GO:1905499"(trichome papilla formation)|interpro:IPR021419	-	comment:mint|function:"Responsible for transcriptional activation of immediate early promoters (alpha genes). Activation requires the formation of a heteromeric complex with the host cell factor. These two proteins then assemble with the octamer motif-binding protein Oct-1 on the cis-acting target sequence: 5'-TAATGARAT-3'"	-	figure legend:f4a|comment:"To test whether plant Med25 also interacts with VP16 in vivo, we performed  immunoprecipitation assays using the N. benthamiana system to co-express the  tagged Arabidopsis proteins (Figure 4A). FLAG-tagged Med25 (FLAG-Med25) was  co-expressed with GFP-FLC, GFP-FLC-VP16, GFP-VP16, or GFP, and the protein  complex including the GFP-tagged proteins was immunoprecipitated with an α-GFP  antibody. FLAG-Med25 was co-immunoprecipitated with the VP16-fused proteins,  GFP-FLC-VP16 and GFP-VP16, but not with GFP or GFP-FLC without VP16 fusion,  indicating that VP16 directly or indirectly interacts with Arabidopsis Med25 in vivo."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4100(nicbe)|taxid:4100(Nicotiana benthamiana)	-	2014/08/05	2014/10/16	rogid:8rAT+y7LSTotMP4XcD+eSyMpff010299	rogid:QxVH3bP188+lGliiHSlp1FrdTy43702	intact-crc:AF5CAEC2FEC2BA51|rigid:OPJfRItpqEL5GD4IjUHqViLAiOg	false	green fluorescent protein tag:?-?|binding-associated region:413-490	flag tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)