#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:E6YC17	uniprotkb:E6YC17	intact:EBI-6912955	intact:EBI-6912955	psi-mi:e6yc17_arahy(display_long)	psi-mi:e6yc17_arahy(display_long)	psi-mi:"MI:0424"(protein kinase assay)	Paul et al. (2014)	pubmed:24996184|imex:IM-22937	taxid:3818(arahy)|taxid:3818("Arachis hypogaea (Peanut)")	taxid:3818(arahy)|taxid:3818("Arachis hypogaea (Peanut)")	psi-mi:"MI:0217"(phosphorylation reaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9631055|imex:IM-22937-1	-	-	psi-mi:"MI:0503"(self)	psi-mi:"MI:0503"(self)	psi-mi:"MI:0503"(self)	psi-mi:"MI:0503"(self)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0004672"(protein kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|rcsb pdb:6M0U|interpro:IPR032675|interpro:IPR011009(Protein kinase-like)|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR024788|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008271(Serine/threonine protein kinase, active site)	go:"GO:0004672"(protein kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|rcsb pdb:6M0U|interpro:IPR032675|interpro:IPR011009(Protein kinase-like)|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR024788|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008271(Serine/threonine protein kinase, active site)	-	-	-	figure legend:F1A F3C|comment:"As indicated in Fig. 3C, no  autophosphorylation was detected in the void fraction (P1) of AhSYMRK. We also tested the  fractions eluting in between P1 and P2 containing higher order oligomeric fractions (data not shown), but autophosphorylation was only detectable in the dimeric fraction of AhSYMRK.  Immunoblot analysis of P1 and P2 using α-pY670 (665–674) and α-pY indicated that both the  oligomeric and dimeric fractions of WT AhSYMRK were autophosphorylated on gatekeeper  tyrosine. This indicated that AhSYMRK could actively autophosphorylate on gatekeeper tyrosine  before oligomerising into inactive higher order structures."|comment:"As indicated in Fig. 1A, the wild-type AhSYMRK and the Y670F mutant actively autophosphorylated but the activity of Y670E kinase was severely affected. Thus a phosphomimic (Y670E) but not a structure-mimic (Y670F) substitution in the gatekeeper position has adverse effect on the catalytic activity of AhSYMRK"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/23	2014/10/16	rogid:cIwtyxCGAn7VEeU+PhA2k1mt3YM3818	rogid:cIwtyxCGAn7VEeU+PhA2k1mt3YM3818	intact-crc:3ACC54028C98699C|rigid:jjw358Z34eZngehEqosgrFySpwo	false	his tag:?-?|mutation decreasing interaction:670-670	his tag:?-?|mutation decreasing interaction:670-670	2	0	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
uniprotkb:E6YC17	uniprotkb:E6YC17	intact:EBI-6912955	intact:EBI-6912955	psi-mi:e6yc17_arahy(display_long)	psi-mi:e6yc17_arahy(display_long)	psi-mi:"MI:0071"(molecular sieving)	Paul et al. (2014)	pubmed:24996184|imex:IM-22937	taxid:3818(arahy)|taxid:3818("Arachis hypogaea (Peanut)")	taxid:3818(arahy)|taxid:3818("Arachis hypogaea (Peanut)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9631204|imex:IM-22937-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0004672"(protein kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|rcsb pdb:6M0U|interpro:IPR032675|interpro:IPR011009(Protein kinase-like)|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR024788|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008271(Serine/threonine protein kinase, active site)	go:"GO:0004672"(protein kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|rcsb pdb:6M0U|interpro:IPR032675|interpro:IPR011009(Protein kinase-like)|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR024788|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008271(Serine/threonine protein kinase, active site)	-	-	-	figure legend:F3A F3B SF1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/23	2014/10/16	rogid:cIwtyxCGAn7VEeU+PhA2k1mt3YM3818	rogid:cIwtyxCGAn7VEeU+PhA2k1mt3YM3818	intact-crc:9C3B9B0A96A67827|rigid:jjw358Z34eZngehEqosgrFySpwo	false	-	-	2	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:E6YC17	uniprotkb:E6YC17	intact:EBI-6912955	intact:EBI-6912955	psi-mi:e6yc17_arahy(display_long)	psi-mi:e6yc17_arahy(display_long)	psi-mi:"MI:0071"(molecular sieving)	Paul et al. (2014)	pubmed:24996184|imex:IM-22937	taxid:3818(arahy)|taxid:3818("Arachis hypogaea (Peanut)")	taxid:3818(arahy)|taxid:3818("Arachis hypogaea (Peanut)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9631242|imex:IM-22937-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0004672"(protein kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|rcsb pdb:6M0U|interpro:IPR032675|interpro:IPR011009(Protein kinase-like)|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR024788|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008271(Serine/threonine protein kinase, active site)	go:"GO:0004672"(protein kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|rcsb pdb:6M0U|interpro:IPR032675|interpro:IPR011009(Protein kinase-like)|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR024788|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008271(Serine/threonine protein kinase, active site)	-	-	-	figure legend:F3A F3B SF1|comment:"WT AhSYMRK eluted  predominantly at around 76 kDa (peak at 16.5 ml) where the catalytically inactive K625E  AhSYMRK eluted at around 40 kDa (peak at 17.28 ml) (Fig. 3A and Supplemental Fig. S1),  suggesting the active WT polypeptide to be stable in a dimeric form. The monomeric form of  K625E polypeptide ruled out the possibility of any influence of the His6-tag on the dimerisation  of AhSYMRK kinase domain (Fig. 3A). We noticed that with increasing concentrations around  75 μM, the WT polypeptide showed an increased tendency to oligomerise and form aggregates  that eluted within void volume. This indicated that the WT AhSYMRK had a propensity to  oligomerise and this propensity was concentration-dependent. Western blot analysis by α-His6 as  well as α-SYMRK confirmed that the void volume fraction as well as other fractions eluting at  higher molecular weight, consisted of aggregates of the WT AhSYMRK polypeptide (Fig. 3B)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/23	2014/10/16	rogid:cIwtyxCGAn7VEeU+PhA2k1mt3YM3818	rogid:cIwtyxCGAn7VEeU+PhA2k1mt3YM3818	intact-crc:2A45C6E5CFE82B10|rigid:5CFR5iUoHPz/TBYqu36uhZ/gkRM	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)