#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:F2D891 uniprotkb:F2D891 intact:EBI-9259445 intact:EBI-9259445 psi-mi:f2d891_horvv(display_long) psi-mi:f2d891_horvv(display_long) psi-mi:"MI:0071"(molecular sieving) Bollivar et al. (2014) pubmed:24661504|imex:IM-22557 taxid:112509(horvd)|taxid:112509("Hordeum vulgare var. distichum (Two-rowed barley)") taxid:112509(horvd)|taxid:112509("Hordeum vulgare var. distichum (Two-rowed barley)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9259443|imex:IM-22557-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR019616|interpro:IPR038409|go:"GO:0042802"(identical protein binding) interpro:IPR019616|interpro:IPR038409|go:"GO:0042802"(identical protein binding) - - - figure legend:f2|comment:"The recombinant Ycf54 protein was observed in several different oligomeric states when separated on gel-permeation chromatography (Fig. 2A), corresponding to approximate monomer (25 kDa; 1.2 x predicted size), trimer (57 kDa; 2.7 x predicted size) and pentamer (108 kDa; 5.1 x predicted size), though the largest molecular weight complex reported here is beyond the resolution of the chromatography column used so the predicted size of this complex should be treated with some caution. When separated on SDS-PAGE the polypeptides present in the multimeric complexes on gel permeation chromatography ran at the same size as the expressed Ycf54 polypeptide (Fig. 2B)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/03/20 2014/10/16 rogid:0n2aGeZ5U4q3TUXeC5+49nDqhl8112509 rogid:0n2aGeZ5U4q3TUXeC5+49nDqhl8112509 intact-crc:B8755DC74AD24F67|rigid:96y+C5PfZ3OSNFbH5nbzKz6fHlE false his tag:n-n his tag:n-n 3 0 psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) uniprotkb:F2D891 uniprotkb:F2D891 intact:EBI-9259445 intact:EBI-9259445 psi-mi:f2d891_horvv(display_long) psi-mi:f2d891_horvv(display_long) psi-mi:"MI:0071"(molecular sieving) Bollivar et al. (2014) pubmed:24661504|imex:IM-22557 taxid:112509(horvd)|taxid:112509("Hordeum vulgare var. distichum (Two-rowed barley)") taxid:112509(horvd)|taxid:112509("Hordeum vulgare var. distichum (Two-rowed barley)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9259450|imex:IM-22557-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR019616|interpro:IPR038409|go:"GO:0042802"(identical protein binding) interpro:IPR019616|interpro:IPR038409|go:"GO:0042802"(identical protein binding) - - - figure legend:f2|comment:"The recombinant Ycf54 protein was observed in several different oligomeric states when separated on gel-permeation chromatography (Fig. 2A), corresponding to approximate monomer (25 kDa; 1.2 x predicted size), trimer (57 kDa; 2.7 x predicted size) and pentamer (108 kDa; 5.1 x predicted size), though the largest molecular weight complex reported here is beyond the resolution of the chromatography column used so the predicted size of this complex should be treated with some caution. When separated on SDS-PAGE the polypeptides present in the multimeric complexes on gel permeation chromatography ran at the same size as the expressed Ycf54 polypeptide (Fig. 2B)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/03/20 2014/10/16 rogid:0n2aGeZ5U4q3TUXeC5+49nDqhl8112509 rogid:0n2aGeZ5U4q3TUXeC5+49nDqhl8112509 intact-crc:95AFF68A3247DA9F|rigid:96y+C5PfZ3OSNFbH5nbzKz6fHlE false his tag:n-n his tag:n-n 5 0 psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)