#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q9Z1E1	uniprotkb:P56817	intact:EBI-920268|uniprotkb:Q9Z1E2|ensembl:ENSRNOP00000001098|ensembl:ENSRNOP00000071275	intact:EBI-2433139|uniprotkb:B0YIU9|uniprotkb:Q9BYB9|uniprotkb:Q9BYC0|uniprotkb:Q9BYC1|uniprotkb:Q9UJT5|uniprotkb:Q9ULS1|uniprotkb:E9PE65|uniprotkb:H7BXJ9|uniprotkb:A0M8W7|ensembl:ENSP00000318585	psi-mi:flot1_rat(display_long)|uniprotkb:Flot1(gene name)|psi-mi:Flot1(display_short)|uniprotkb:Reg2(gene name synonym)|uniprotkb:Reggie-2(gene name synonym)	psi-mi:bace1_human(display_long)|uniprotkb:BACE(gene name synonym)|uniprotkb:KIAA1149(gene name synonym)|uniprotkb:Beta-site amyloid precursor protein cleaving enzyme 1(gene name synonym)|uniprotkb:Membrane-associated aspartic protease 2(gene name synonym)|uniprotkb:Memapsin-2(gene name synonym)|uniprotkb:Aspartyl protease 2(gene name synonym)|uniprotkb:BACE1(gene name)|psi-mi:BACE1(display_short)	psi-mi:"MI:0096"(pull down)	John et al. (2014)	pubmed:24612608|imex:IM-22281	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9213775|imex:IM-22281-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rgd:70991|refseq:NP_073192.2|refseq:XP_006256086.1|ensembl:ENSRNOG00000000826(gene)|ensembl:ENSRNOT00000001098(transcript)|ensembl:ENSRNOT00000090039(transcript)|go:"GO:0001765"(membrane raft assembly)|go:"GO:0001819"(positive regulation of cytokine production)|go:"GO:0001931"(uropod)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0002020"(protease binding)|go:"GO:0002090"(regulation of receptor internalization)|go:"GO:0005768"(endosome)|go:"GO:0005769"(early endosome)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005886"(plasma membrane)|go:"GO:0005901"(caveola)|go:"GO:0005911"(cell-cell junction)|go:"GO:0005912"(adherens junction)|go:"GO:0007409"(axonogenesis)|go:"GO:0008180"(COP9 signalosome)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016600"(flotillin complex)|go:"GO:0022617"(extracellular matrix disassembly)|go:"GO:0030027"(lamellipodium)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032092"(positive regulation of protein binding)|go:"GO:0032226"(positive regulation of synaptic transmission, dopaminergic)|go:"GO:0032728"(positive regulation of interferon-beta production)|go:"GO:0033227"(dsRNA transport)|go:"GO:0034116"(positive regulation of heterotypic cell-cell adhesion)|go:"GO:0034141"(positive regulation of toll-like receptor 3 signaling pathway)|go:"GO:0034451"(centriolar satellite)|go:"GO:0034976"(response to endoplasmic reticulum stress)|go:"GO:0035023"(regulation of Rho protein signal transduction)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0044291"(cell-cell contact zone)|go:"GO:0044853"(plasma membrane raft)|go:"GO:0044854"(plasma membrane raft assembly)|go:"GO:0045121"(membrane raft)|go:"GO:0045202"(synapse)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048643"(positive regulation of skeletal muscle tissue development)|go:"GO:0048786"(presynaptic active zone)|go:"GO:0050821"(protein stabilization)|go:"GO:0051092"(positive regulation of NF-kappaB transcription factor activity)|go:"GO:0051580"(regulation of neurotransmitter uptake)|go:"GO:0060355"(positive regulation of cell adhesion molecule production)|go:"GO:0070528"(protein kinase C signaling)|go:"GO:0071360"(cellular response to exogenous dsRNA)|go:"GO:0072659"(protein localization to plasma membrane)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098793"(presynapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:1901741"(positive regulation of myoblast fusion)|go:"GO:1901890"(positive regulation of cell junction assembly)|go:"GO:1903044"(protein localization to membrane raft)|go:"GO:2000049"(positive regulation of cell-cell adhesion mediated by cadherin)|interpro:IPR001107(Band 7 protein)|interpro:IPR027705|interpro:IPR031905|interpro:IPR036013|mint:Q9Z1E1|reactome:R-RNO-5213460|reactome:R-RNO-5675482|reactome:R-RNO-8849932|reactome:R-RNO-8980692|reactome:R-RNO-9013026|reactome:R-RNO-9013106	refseq:NP_001193978.1|refseq:NP_036236.1|refseq:NP_620427.1|refseq:NP_620428.1|refseq:NP_620429.1|dip:DIP-41388N|ensembl:ENSG00000186318(gene)|ensembl:ENST00000313005(transcript)|go:"GO:0001540"(amyloid-beta binding)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004190"(aspartic-type endopeptidase activity)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005771"(multivesicular body)|go:"GO:0005788"(endoplasmic reticulum lumen)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006508"(proteolysis)|go:"GO:0006509"(membrane protein ectodomain proteolysis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0008233"(peptidase activity)|go:"GO:0008798"(beta-aspartyl-peptidase activity)|go:"GO:0009314"(response to radiation)|go:"GO:0009986"(cell surface)|go:"GO:0010008"(endosome membrane)|go:"GO:0010288"(response to lead ion)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016485"(protein processing)|go:"GO:0019899"(enzyme binding)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0034205"(amyloid-beta formation)|go:"GO:0042987"(amyloid precursor protein catabolic process)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043525"(positive regulation of neuron apoptotic process)|go:"GO:0045121"(membrane raft)|go:"GO:0050435"(amyloid-beta metabolic process)|go:"GO:0050966"(detection of mechanical stimulus involved in sensory perception of pain)|go:"GO:0055037"(recycling endosome)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0070931"(Golgi-associated vesicle lumen)|go:"GO:0071280"(cellular response to copper ion)|go:"GO:0071287"(cellular response to manganese ion)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:1904646"(cellular response to amyloid-beta)|go:"GO:1990000"(amyloid fibril formation)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001461(Peptidase A1)|interpro:IPR001969(Peptidase aspartic, active site)|interpro:IPR009119(Peptidase A1, beta-site APP cleaving enzyme, BACE)|interpro:IPR009120(Peptidase A1, beta-site APP cleaving enzyme 1, BACE 1)|interpro:IPR021109|interpro:IPR033121|interpro:IPR033874|mint:P56817|rcsb pdb:1FKN|rcsb pdb:1M4H|rcsb pdb:1PY1|rcsb pdb:1SGZ|rcsb pdb:1TQF|rcsb pdb:1UJJ|rcsb pdb:1UJK|rcsb pdb:1W50|rcsb pdb:1W51|rcsb pdb:1XN2|rcsb pdb:1XN3|rcsb pdb:1XS7|rcsb pdb:1YM2|rcsb pdb:1YM4|rcsb pdb:2B8L|rcsb pdb:2B8V|rcsb pdb:2F3E|rcsb pdb:2F3F|rcsb pdb:2FDP|rcsb pdb:2G94|rcsb pdb:2HIZ|rcsb pdb:2HM1|rcsb pdb:2IQG|rcsb pdb:2IRZ|rcsb pdb:2IS0|rcsb pdb:2NTR|rcsb pdb:2OAH|rcsb pdb:2OF0|rcsb pdb:2OHK|rcsb pdb:2OHL|rcsb pdb:2OHM|rcsb pdb:2OHN|rcsb pdb:2OHP|rcsb pdb:2OHQ|rcsb pdb:2OHR|rcsb pdb:2OHS|rcsb pdb:2OHT|rcsb pdb:2OHU|rcsb pdb:2P4J|rcsb pdb:2P83|rcsb pdb:2P8H|rcsb pdb:2PH6|rcsb pdb:2PH8|rcsb pdb:2Q11|rcsb pdb:2Q15|rcsb pdb:2QK5|rcsb pdb:2QMD|rcsb pdb:2QMF|rcsb pdb:2QMG|rcsb pdb:2QP8|rcsb pdb:2QU2|rcsb pdb:2QU3|rcsb pdb:2QZK|rcsb pdb:2QZL|rcsb pdb:2VA5|rcsb pdb:2VA6|rcsb pdb:2VA7|rcsb pdb:2VIE|rcsb pdb:2VIJ|rcsb pdb:2VIY|rcsb pdb:2VIZ|rcsb pdb:2VJ6|rcsb pdb:2VJ7|rcsb pdb:2VJ9|rcsb pdb:2VKM|rcsb pdb:2VNM|rcsb pdb:2VNN|rcsb pdb:2WEZ|rcsb pdb:2WF0|rcsb pdb:2WF1|rcsb pdb:2WF2|rcsb pdb:2WF3|rcsb pdb:2WF4|rcsb pdb:2WJO|rcsb pdb:2XFI|rcsb pdb:2XFJ|rcsb pdb:2XFK|rcsb pdb:2ZDZ|rcsb pdb:2ZE1|rcsb pdb:2ZHR|rcsb pdb:2ZHS|rcsb pdb:2ZHT|rcsb pdb:2ZHU|rcsb pdb:2ZHV|rcsb pdb:2ZJH|rcsb pdb:2ZJI|rcsb pdb:2ZJJ|rcsb pdb:2ZJK|rcsb pdb:2ZJL|rcsb pdb:2ZJM|rcsb pdb:2ZJN|rcsb pdb:3BRA|rcsb pdb:3BUF|rcsb pdb:3BUG|rcsb pdb:3BUH|rcsb pdb:3CIB|rcsb pdb:3CIC|rcsb pdb:3CID|rcsb pdb:3CKP|rcsb pdb:3CKR|rcsb pdb:3DM6|rcsb pdb:3DUY|rcsb pdb:3DV1|rcsb pdb:3DV5|rcsb pdb:3EXO|rcsb pdb:3FKT|rcsb pdb:3H0B|rcsb pdb:3HVG|rcsb pdb:3HW1|rcsb pdb:3I25|rcsb pdb:3IGB|rcsb pdb:3IN3|rcsb pdb:3IN4|rcsb pdb:3IND|rcsb pdb:3INE|rcsb pdb:3INF|rcsb pdb:3INH|rcsb pdb:3IVH|rcsb pdb:3IVI|rcsb pdb:3IXJ|rcsb pdb:3IXK|rcsb pdb:3K5C|rcsb pdb:3K5D|rcsb pdb:3K5F|rcsb pdb:3K5G|rcsb pdb:3KMX|rcsb pdb:3KMY|rcsb pdb:3KN0|rcsb pdb:3KYR|rcsb pdb:3L38|rcsb pdb:3L3A|rcsb pdb:3L58|rcsb pdb:3L59|rcsb pdb:3L5B|rcsb pdb:3L5C|rcsb pdb:3L5D|rcsb pdb:3L5E|rcsb pdb:3L5F|rcsb pdb:3LHG|rcsb pdb:3LNK|rcsb pdb:3LPI|rcsb pdb:3LPJ|rcsb pdb:3LPK|rcsb pdb:3MSJ|rcsb pdb:3MSK|rcsb pdb:3MSL|rcsb pdb:3N4L|rcsb pdb:3NSH|rcsb pdb:3OHF|rcsb pdb:3OHH|rcsb pdb:3OOZ|rcsb pdb:3PI5|rcsb pdb:3QBH|rcsb pdb:3QI1|rcsb pdb:3R1G|rcsb pdb:3R2F|rcsb pdb:3RSV|rcsb pdb:3RSX|rcsb pdb:3RTH|rcsb pdb:3RTM|rcsb pdb:3RTN|rcsb pdb:3RU1|rcsb pdb:3RVI|rcsb pdb:3S2O|rcsb pdb:3S7L|rcsb pdb:3S7M|rcsb pdb:3SKF|rcsb pdb:3TPJ|rcsb pdb:3TPL|rcsb pdb:3TPP|rcsb pdb:3TPR|rcsb pdb:3U6A|rcsb pdb:3UDH|rcsb pdb:3UDJ|rcsb pdb:3UDK|rcsb pdb:3UDM|rcsb pdb:3UDN|rcsb pdb:3UDP|rcsb pdb:3UDQ|rcsb pdb:3UDR|rcsb pdb:3UDY|rcsb pdb:3UFL|rcsb pdb:3UQP|rcsb pdb:3UQR|rcsb pdb:3UQU|rcsb pdb:3UQW|rcsb pdb:3UQX|rcsb pdb:3VEU|rcsb pdb:3VF3|rcsb pdb:3VG1|rcsb pdb:3VV6|rcsb pdb:3VV7|rcsb pdb:3VV8|rcsb pdb:3WB4|rcsb pdb:3WB5|rcsb pdb:3ZMG|rcsb pdb:3ZOV|rcsb pdb:4ACU|rcsb pdb:4ACX|rcsb pdb:4AZY|rcsb pdb:4B00|rcsb pdb:4B05|rcsb pdb:4B0Q|rcsb pdb:4B1C|rcsb pdb:4B1D|rcsb pdb:4B1E|rcsb pdb:4B70|rcsb pdb:4B72|rcsb pdb:4B77|rcsb pdb:4B78|rcsb pdb:4BEK|rcsb pdb:4BFD|rcsb pdb:4D83|rcsb pdb:4D85|rcsb pdb:4D88|rcsb pdb:4D89|rcsb pdb:4D8C|rcsb pdb:4DH6|rcsb pdb:4DI2|rcsb pdb:4DJU|rcsb pdb:4DJV|rcsb pdb:4DJW|rcsb pdb:4DJX|rcsb pdb:4DJY|rcsb pdb:4DPF|rcsb pdb:4DPI|rcsb pdb:4DUS|rcsb pdb:4DV9|rcsb pdb:4DVF|rcsb pdb:4EWO|rcsb pdb:4EXG|rcsb pdb:4FCO|rcsb pdb:4FGX|rcsb pdb:4FM7|rcsb pdb:4FM8|rcsb pdb:4FRI|rcsb pdb:4FRJ|rcsb pdb:4FRK|rcsb pdb:4FRS|rcsb pdb:4FS4|rcsb pdb:4FSE|rcsb pdb:4FSL|rcsb pdb:4GID|rcsb pdb:4GMI|rcsb pdb:4H1E|rcsb pdb:4H3F|rcsb pdb:4H3G|rcsb pdb:4H3I|rcsb pdb:4H3J|rcsb pdb:4HA5|rcsb pdb:4HZT|rcsb pdb:4I0D|rcsb pdb:4I0E|rcsb pdb:4I0F|rcsb pdb:4I0G|rcsb pdb:4I0H|rcsb pdb:4I0I|rcsb pdb:4I0J|rcsb pdb:4I0Z|rcsb pdb:4I10|rcsb pdb:4I11|rcsb pdb:3SKG|rcsb pdb:4I12|rcsb pdb:4I1C|rcsb pdb:4IVS|rcsb pdb:4IVT|rcsb pdb:4J0P|rcsb pdb:4J0T|rcsb pdb:4J0V|rcsb pdb:4J0Y|rcsb pdb:4J0Z|rcsb pdb:4J17|rcsb pdb:4J1C|rcsb pdb:4J1E|rcsb pdb:4J1F|rcsb pdb:4J1H|rcsb pdb:4J1I|rcsb pdb:4J1K|rcsb pdb:4JOO|rcsb pdb:4JP9|rcsb pdb:4JPC|rcsb pdb:4JPE|rcsb pdb:4K8S|rcsb pdb:4K9H|rcsb pdb:4KE0|rcsb pdb:4KE1|rcsb pdb:4L7G|rcsb pdb:4L7H|rcsb pdb:4L7J|rcsb pdb:4LC7|rcsb pdb:4LXA|rcsb pdb:4LXK|rcsb pdb:4LXM|rcsb pdb:4N00|rcsb pdb:4PZW|rcsb pdb:4PZX|rcsb pdb:4R5N|rcsb pdb:4R8Y|rcsb pdb:4R91|rcsb pdb:4R92|rcsb pdb:4R93|rcsb pdb:4R95|rcsb pdb:4RCD|rcsb pdb:4RCE|rcsb pdb:4RCF|rcsb pdb:4RRN|rcsb pdb:4RRO|rcsb pdb:4RRS|rcsb pdb:4TRW|rcsb pdb:4TRY|rcsb pdb:4TRZ|rcsb pdb:4WTU|rcsb pdb:4WY1|rcsb pdb:4WY6|rcsb pdb:4X2L|rcsb pdb:4X7I|rcsb pdb:4XKX|rcsb pdb:4XXS|rcsb pdb:4YBI|rcsb pdb:4ZPE|rcsb pdb:4ZPF|rcsb pdb:4ZPG|rcsb pdb:4ZSM|rcsb pdb:4ZSP|rcsb pdb:4ZSQ|rcsb pdb:4ZSR|rcsb pdb:5CLM|rcsb pdb:5DQC|rcsb pdb:5ENK|rcsb pdb:5ENM|rcsb pdb:5EZX|rcsb pdb:5EZZ|rcsb pdb:5F00|rcsb pdb:5F01|rcsb pdb:5HD0|rcsb pdb:5HDU|rcsb pdb:5HDV|rcsb pdb:5HDX|rcsb pdb:5HDZ|rcsb pdb:5HE4|rcsb pdb:5HE5|rcsb pdb:5HE7|rcsb pdb:5HTZ|rcsb pdb:5HU0|rcsb pdb:5HU1|rcsb pdb:5I3V|rcsb pdb:5I3W|rcsb pdb:5I3X|rcsb pdb:5I3Y|rcsb pdb:5IE1|rcsb pdb:5KQF|rcsb pdb:5KR8|rcsb pdb:5MBW|rcsb pdb:5MCO|rcsb pdb:5MCQ|rcsb pdb:5MXD|rcsb pdb:5QCO|rcsb pdb:5QCP|rcsb pdb:5QCQ|rcsb pdb:5QCR|rcsb pdb:5QCS|rcsb pdb:5QCT|rcsb pdb:5QCU|rcsb pdb:5QCV|rcsb pdb:5QCW|rcsb pdb:5QCX|rcsb pdb:5QCY|rcsb pdb:5QCZ|rcsb pdb:5QD0|rcsb pdb:5QD1|rcsb pdb:5QD2|rcsb pdb:5QD3|rcsb pdb:5QD4|rcsb pdb:5QD5|rcsb pdb:5QD6|rcsb pdb:5QD7|rcsb pdb:5QD8|rcsb pdb:5QD9|rcsb pdb:5QDA|rcsb pdb:5QDB|rcsb pdb:5QDC|rcsb pdb:5QDD|rcsb pdb:5T1U|rcsb pdb:5T1W|rcsb pdb:5TOL|rcsb pdb:5UYU|rcsb pdb:5V0N|rcsb pdb:5YGX|rcsb pdb:5YGY|rcsb pdb:6BFD|rcsb pdb:6BFE|rcsb pdb:6BFW|rcsb pdb:6BFX|rcsb pdb:6C2I|rcsb pdb:6DHC|rcsb pdb:6DMI|rcsb pdb:6E3Z|rcsb pdb:6EJ2|rcsb pdb:6EJ3|rcsb pdb:6EQM|rcsb pdb:6FGY|rcsb pdb:6JSE|rcsb pdb:6JSF|rcsb pdb:6JSG|rcsb pdb:6JSN|rcsb pdb:6JT3|rcsb pdb:6JT4|rcsb pdb:6NV7|rcsb pdb:6NV9|rcsb pdb:6NW3|rcsb pdb:6OD6|rcsb pdb:6PZ4|rcsb pdb:6UVP|rcsb pdb:6UVV|rcsb pdb:6UVY|rcsb pdb:6UWP|rcsb pdb:6UWV|rcsb pdb:6WNY|rcsb pdb:7B1E|rcsb pdb:7B1P|rcsb pdb:7B1Q|rcsb pdb:7D2V|rcsb pdb:7D2X|rcsb pdb:7D5A|rcsb pdb:7DCZ|reactome:R-HSA-977225	-	-	crc64:377CE4C824ACEF05	figure legend:f1d|comment:"To dissect the role of flotillins in BACE1 binding in more detail and to test if the  interaction is a direct one, we performed direct pulldown assays using bacterially  expressed, purified proteins (Figure 1D). Purified flotillin-1 again strongly bound to  the WT BACE1-GST tail, whereas no binding was observed with the LLAA tail. Only  a very weak binding that was close to the GST background level was observed with  purified flotillin-2. Taken together, these data show that flotillin-1 directly interacts  with the cytoplasmic tail of BACE1 and that this interaction is mediated by the well  known dileucine sorting motif in BACE1"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/21	2017/06/28	rogid:zQd1GziJRcfE7u4/b/800Dotg8A10116	rogid:3oEbxDI/Eu5LwK/3QKtUhS41n3o9606	intact-crc:4C2F5609EDD5F263|rigid:Nh++VvvA1VwewfoFejZkxnEB2W4	false	-	glutathione s tranferase tag:?-?|binding-associated region:479-501|mutation disrupting interaction:499-500	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q14254	uniprotkb:P56817	intact:EBI-348613|ensembl:ENSP00000378368	intact:EBI-2433139|uniprotkb:B0YIU9|uniprotkb:Q9BYB9|uniprotkb:Q9BYC0|uniprotkb:Q9BYC1|uniprotkb:Q9UJT5|uniprotkb:Q9ULS1|uniprotkb:E9PE65|uniprotkb:H7BXJ9|uniprotkb:A0M8W7|ensembl:ENSP00000318585	psi-mi:flot2_human(display_long)|uniprotkb:FLOT2(gene name)|psi-mi:FLOT2(display_short)|uniprotkb:ESA1(gene name synonym)|uniprotkb:M17S1(gene name synonym)|uniprotkb:Epidermal surface antigen(gene name synonym)|uniprotkb:Membrane component chromosome 17 surface marker 1(gene name synonym)	psi-mi:bace1_human(display_long)|uniprotkb:BACE(gene name synonym)|uniprotkb:KIAA1149(gene name synonym)|uniprotkb:Beta-site amyloid precursor protein cleaving enzyme 1(gene name synonym)|uniprotkb:Membrane-associated aspartic protease 2(gene name synonym)|uniprotkb:Memapsin-2(gene name synonym)|uniprotkb:Aspartyl protease 2(gene name synonym)|uniprotkb:BACE1(gene name)|psi-mi:BACE1(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	John et al. (2014)	pubmed:24612608|imex:IM-22281	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9213800|imex:IM-22281-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_004466.2|ensembl:ENSG00000132589(gene)|go:"GO:0008544"(epidermis development)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0016020"(membrane)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016600"(flotillin complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031982"(vesicle)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0044291"(cell-cell contact zone)|go:"GO:0044860"(protein localization to plasma membrane raft)|go:"GO:0045661"(regulation of myoblast differentiation)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050821"(protein stabilization)|go:"GO:0051092"(positive regulation of NF-kappaB transcription factor activity)|go:"GO:0070062"(extracellular exosome)|go:"GO:0072659"(protein localization to plasma membrane)|go:"GO:0098937"(anterograde dendritic transport)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099029"(anchored component of presynaptic active zone membrane)|ensembl:ENST00000394908(transcript)|go:"GO:0001765"(membrane raft assembly)|go:"GO:0001931"(uropod)|go:"GO:0002020"(protease binding)|go:"GO:0002080"(acrosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0005901"(caveola)|go:"GO:0005912"(adherens junction)|go:"GO:0005925"(focal adhesion)|go:"GO:0007155"(cell adhesion)|go:"GO:0099072"(regulation of postsynaptic membrane neurotransmitter receptor levels)|go:"GO:1902992"(negative regulation of amyloid precursor protein catabolic process)|go:"GO:1903905"(positive regulation of establishment of T cell polarity)|interpro:IPR001107(Band 7 protein)|interpro:IPR027705|interpro:IPR031905|interpro:IPR036013|mint:Q14254|reactome:R-HSA-5213460|reactome:R-HSA-5675482|reactome:R-HSA-8849932|reactome:R-HSA-8980692|reactome:R-HSA-9013026|reactome:R-HSA-9013106|reactome:R-HSA-9696264|reactome:R-HSA-9696273|refseq:XP_016879883.1|refseq:XP_016879884.1	refseq:NP_001193978.1|refseq:NP_036236.1|refseq:NP_620427.1|refseq:NP_620428.1|refseq:NP_620429.1|dip:DIP-41388N|ensembl:ENSG00000186318(gene)|ensembl:ENST00000313005(transcript)|go:"GO:0001540"(amyloid-beta binding)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004190"(aspartic-type endopeptidase activity)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005771"(multivesicular body)|go:"GO:0005788"(endoplasmic reticulum lumen)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006508"(proteolysis)|go:"GO:0006509"(membrane protein ectodomain proteolysis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0008233"(peptidase activity)|go:"GO:0008798"(beta-aspartyl-peptidase activity)|go:"GO:0009314"(response to radiation)|go:"GO:0009986"(cell surface)|go:"GO:0010008"(endosome membrane)|go:"GO:0010288"(response to lead ion)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016485"(protein processing)|go:"GO:0019899"(enzyme binding)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0034205"(amyloid-beta formation)|go:"GO:0042987"(amyloid precursor protein catabolic process)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043525"(positive regulation of neuron apoptotic process)|go:"GO:0045121"(membrane raft)|go:"GO:0050435"(amyloid-beta metabolic process)|go:"GO:0050966"(detection of mechanical stimulus involved in sensory perception of pain)|go:"GO:0055037"(recycling endosome)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0070931"(Golgi-associated vesicle lumen)|go:"GO:0071280"(cellular response to copper ion)|go:"GO:0071287"(cellular response to manganese ion)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:1904646"(cellular response to amyloid-beta)|go:"GO:1990000"(amyloid fibril formation)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001461(Peptidase A1)|interpro:IPR001969(Peptidase aspartic, active site)|interpro:IPR009119(Peptidase A1, beta-site APP cleaving enzyme, BACE)|interpro:IPR009120(Peptidase A1, beta-site APP cleaving enzyme 1, BACE 1)|interpro:IPR021109|interpro:IPR033121|interpro:IPR033874|mint:P56817|rcsb pdb:1FKN|rcsb pdb:1M4H|rcsb pdb:1PY1|rcsb pdb:1SGZ|rcsb pdb:1TQF|rcsb pdb:1UJJ|rcsb pdb:1UJK|rcsb pdb:1W50|rcsb pdb:1W51|rcsb pdb:1XN2|rcsb pdb:1XN3|rcsb pdb:1XS7|rcsb pdb:1YM2|rcsb pdb:1YM4|rcsb pdb:2B8L|rcsb pdb:2B8V|rcsb pdb:2F3E|rcsb pdb:2F3F|rcsb pdb:2FDP|rcsb pdb:2G94|rcsb pdb:2HIZ|rcsb pdb:2HM1|rcsb pdb:2IQG|rcsb pdb:2IRZ|rcsb pdb:2IS0|rcsb pdb:2NTR|rcsb pdb:2OAH|rcsb pdb:2OF0|rcsb pdb:2OHK|rcsb pdb:2OHL|rcsb pdb:2OHM|rcsb pdb:2OHN|rcsb pdb:2OHP|rcsb pdb:2OHQ|rcsb pdb:2OHR|rcsb pdb:2OHS|rcsb pdb:2OHT|rcsb pdb:2OHU|rcsb pdb:2P4J|rcsb pdb:2P83|rcsb pdb:2P8H|rcsb pdb:2PH6|rcsb pdb:2PH8|rcsb pdb:2Q11|rcsb pdb:2Q15|rcsb pdb:2QK5|rcsb pdb:2QMD|rcsb pdb:2QMF|rcsb pdb:2QMG|rcsb pdb:2QP8|rcsb pdb:2QU2|rcsb pdb:2QU3|rcsb pdb:2QZK|rcsb pdb:2QZL|rcsb pdb:2VA5|rcsb pdb:2VA6|rcsb pdb:2VA7|rcsb pdb:2VIE|rcsb pdb:2VIJ|rcsb pdb:2VIY|rcsb pdb:2VIZ|rcsb pdb:2VJ6|rcsb pdb:2VJ7|rcsb pdb:2VJ9|rcsb pdb:2VKM|rcsb pdb:2VNM|rcsb pdb:2VNN|rcsb pdb:2WEZ|rcsb pdb:2WF0|rcsb pdb:2WF1|rcsb pdb:2WF2|rcsb pdb:2WF3|rcsb pdb:2WF4|rcsb pdb:2WJO|rcsb pdb:2XFI|rcsb pdb:2XFJ|rcsb pdb:2XFK|rcsb pdb:2ZDZ|rcsb pdb:2ZE1|rcsb pdb:2ZHR|rcsb pdb:2ZHS|rcsb pdb:2ZHT|rcsb pdb:2ZHU|rcsb pdb:2ZHV|rcsb pdb:2ZJH|rcsb pdb:2ZJI|rcsb pdb:2ZJJ|rcsb pdb:2ZJK|rcsb pdb:2ZJL|rcsb pdb:2ZJM|rcsb pdb:2ZJN|rcsb pdb:3BRA|rcsb pdb:3BUF|rcsb pdb:3BUG|rcsb pdb:3BUH|rcsb pdb:3CIB|rcsb pdb:3CIC|rcsb pdb:3CID|rcsb pdb:3CKP|rcsb pdb:3CKR|rcsb pdb:3DM6|rcsb pdb:3DUY|rcsb pdb:3DV1|rcsb pdb:3DV5|rcsb pdb:3EXO|rcsb pdb:3FKT|rcsb pdb:3H0B|rcsb pdb:3HVG|rcsb pdb:3HW1|rcsb pdb:3I25|rcsb pdb:3IGB|rcsb pdb:3IN3|rcsb pdb:3IN4|rcsb pdb:3IND|rcsb pdb:3INE|rcsb pdb:3INF|rcsb pdb:3INH|rcsb pdb:3IVH|rcsb pdb:3IVI|rcsb pdb:3IXJ|rcsb pdb:3IXK|rcsb pdb:3K5C|rcsb pdb:3K5D|rcsb pdb:3K5F|rcsb pdb:3K5G|rcsb pdb:3KMX|rcsb pdb:3KMY|rcsb pdb:3KN0|rcsb pdb:3KYR|rcsb pdb:3L38|rcsb pdb:3L3A|rcsb pdb:3L58|rcsb pdb:3L59|rcsb pdb:3L5B|rcsb pdb:3L5C|rcsb pdb:3L5D|rcsb pdb:3L5E|rcsb pdb:3L5F|rcsb pdb:3LHG|rcsb pdb:3LNK|rcsb pdb:3LPI|rcsb pdb:3LPJ|rcsb pdb:3LPK|rcsb pdb:3MSJ|rcsb pdb:3MSK|rcsb pdb:3MSL|rcsb pdb:3N4L|rcsb pdb:3NSH|rcsb pdb:3OHF|rcsb pdb:3OHH|rcsb pdb:3OOZ|rcsb pdb:3PI5|rcsb pdb:3QBH|rcsb pdb:3QI1|rcsb pdb:3R1G|rcsb pdb:3R2F|rcsb pdb:3RSV|rcsb pdb:3RSX|rcsb pdb:3RTH|rcsb pdb:3RTM|rcsb pdb:3RTN|rcsb pdb:3RU1|rcsb pdb:3RVI|rcsb pdb:3S2O|rcsb pdb:3S7L|rcsb pdb:3S7M|rcsb pdb:3SKF|rcsb pdb:3TPJ|rcsb pdb:3TPL|rcsb pdb:3TPP|rcsb pdb:3TPR|rcsb pdb:3U6A|rcsb pdb:3UDH|rcsb pdb:3UDJ|rcsb pdb:3UDK|rcsb pdb:3UDM|rcsb pdb:3UDN|rcsb pdb:3UDP|rcsb pdb:3UDQ|rcsb pdb:3UDR|rcsb pdb:3UDY|rcsb pdb:3UFL|rcsb pdb:3UQP|rcsb pdb:3UQR|rcsb pdb:3UQU|rcsb pdb:3UQW|rcsb pdb:3UQX|rcsb pdb:3VEU|rcsb pdb:3VF3|rcsb pdb:3VG1|rcsb pdb:3VV6|rcsb pdb:3VV7|rcsb pdb:3VV8|rcsb pdb:3WB4|rcsb pdb:3WB5|rcsb pdb:3ZMG|rcsb pdb:3ZOV|rcsb pdb:4ACU|rcsb pdb:4ACX|rcsb pdb:4AZY|rcsb pdb:4B00|rcsb pdb:4B05|rcsb pdb:4B0Q|rcsb pdb:4B1C|rcsb pdb:4B1D|rcsb pdb:4B1E|rcsb pdb:4B70|rcsb pdb:4B72|rcsb pdb:4B77|rcsb pdb:4B78|rcsb pdb:4BEK|rcsb pdb:4BFD|rcsb pdb:4D83|rcsb pdb:4D85|rcsb pdb:4D88|rcsb pdb:4D89|rcsb pdb:4D8C|rcsb pdb:4DH6|rcsb pdb:4DI2|rcsb pdb:4DJU|rcsb pdb:4DJV|rcsb pdb:4DJW|rcsb pdb:4DJX|rcsb pdb:4DJY|rcsb pdb:4DPF|rcsb pdb:4DPI|rcsb pdb:4DUS|rcsb pdb:4DV9|rcsb pdb:4DVF|rcsb pdb:4EWO|rcsb pdb:4EXG|rcsb pdb:4FCO|rcsb pdb:4FGX|rcsb pdb:4FM7|rcsb pdb:4FM8|rcsb pdb:4FRI|rcsb pdb:4FRJ|rcsb pdb:4FRK|rcsb pdb:4FRS|rcsb pdb:4FS4|rcsb pdb:4FSE|rcsb pdb:4FSL|rcsb pdb:4GID|rcsb pdb:4GMI|rcsb pdb:4H1E|rcsb pdb:4H3F|rcsb pdb:4H3G|rcsb pdb:4H3I|rcsb pdb:4H3J|rcsb pdb:4HA5|rcsb pdb:4HZT|rcsb pdb:4I0D|rcsb pdb:4I0E|rcsb pdb:4I0F|rcsb pdb:4I0G|rcsb pdb:4I0H|rcsb pdb:4I0I|rcsb pdb:4I0J|rcsb pdb:4I0Z|rcsb pdb:4I10|rcsb pdb:4I11|rcsb pdb:3SKG|rcsb pdb:4I12|rcsb pdb:4I1C|rcsb pdb:4IVS|rcsb pdb:4IVT|rcsb pdb:4J0P|rcsb pdb:4J0T|rcsb pdb:4J0V|rcsb pdb:4J0Y|rcsb pdb:4J0Z|rcsb pdb:4J17|rcsb pdb:4J1C|rcsb pdb:4J1E|rcsb pdb:4J1F|rcsb pdb:4J1H|rcsb pdb:4J1I|rcsb pdb:4J1K|rcsb pdb:4JOO|rcsb pdb:4JP9|rcsb pdb:4JPC|rcsb pdb:4JPE|rcsb pdb:4K8S|rcsb pdb:4K9H|rcsb pdb:4KE0|rcsb pdb:4KE1|rcsb pdb:4L7G|rcsb pdb:4L7H|rcsb pdb:4L7J|rcsb pdb:4LC7|rcsb pdb:4LXA|rcsb pdb:4LXK|rcsb pdb:4LXM|rcsb pdb:4N00|rcsb pdb:4PZW|rcsb pdb:4PZX|rcsb pdb:4R5N|rcsb pdb:4R8Y|rcsb pdb:4R91|rcsb pdb:4R92|rcsb pdb:4R93|rcsb pdb:4R95|rcsb pdb:4RCD|rcsb pdb:4RCE|rcsb pdb:4RCF|rcsb pdb:4RRN|rcsb pdb:4RRO|rcsb pdb:4RRS|rcsb pdb:4TRW|rcsb pdb:4TRY|rcsb pdb:4TRZ|rcsb pdb:4WTU|rcsb pdb:4WY1|rcsb pdb:4WY6|rcsb pdb:4X2L|rcsb pdb:4X7I|rcsb pdb:4XKX|rcsb pdb:4XXS|rcsb pdb:4YBI|rcsb pdb:4ZPE|rcsb pdb:4ZPF|rcsb pdb:4ZPG|rcsb pdb:4ZSM|rcsb pdb:4ZSP|rcsb pdb:4ZSQ|rcsb pdb:4ZSR|rcsb pdb:5CLM|rcsb pdb:5DQC|rcsb pdb:5ENK|rcsb pdb:5ENM|rcsb pdb:5EZX|rcsb pdb:5EZZ|rcsb pdb:5F00|rcsb pdb:5F01|rcsb pdb:5HD0|rcsb pdb:5HDU|rcsb pdb:5HDV|rcsb pdb:5HDX|rcsb pdb:5HDZ|rcsb pdb:5HE4|rcsb pdb:5HE5|rcsb pdb:5HE7|rcsb pdb:5HTZ|rcsb pdb:5HU0|rcsb pdb:5HU1|rcsb pdb:5I3V|rcsb pdb:5I3W|rcsb pdb:5I3X|rcsb pdb:5I3Y|rcsb pdb:5IE1|rcsb pdb:5KQF|rcsb pdb:5KR8|rcsb pdb:5MBW|rcsb pdb:5MCO|rcsb pdb:5MCQ|rcsb pdb:5MXD|rcsb pdb:5QCO|rcsb pdb:5QCP|rcsb pdb:5QCQ|rcsb pdb:5QCR|rcsb pdb:5QCS|rcsb pdb:5QCT|rcsb pdb:5QCU|rcsb pdb:5QCV|rcsb pdb:5QCW|rcsb pdb:5QCX|rcsb pdb:5QCY|rcsb pdb:5QCZ|rcsb pdb:5QD0|rcsb pdb:5QD1|rcsb pdb:5QD2|rcsb pdb:5QD3|rcsb pdb:5QD4|rcsb pdb:5QD5|rcsb pdb:5QD6|rcsb pdb:5QD7|rcsb pdb:5QD8|rcsb pdb:5QD9|rcsb pdb:5QDA|rcsb pdb:5QDB|rcsb pdb:5QDC|rcsb pdb:5QDD|rcsb pdb:5T1U|rcsb pdb:5T1W|rcsb pdb:5TOL|rcsb pdb:5UYU|rcsb pdb:5V0N|rcsb pdb:5YGX|rcsb pdb:5YGY|rcsb pdb:6BFD|rcsb pdb:6BFE|rcsb pdb:6BFW|rcsb pdb:6BFX|rcsb pdb:6C2I|rcsb pdb:6DHC|rcsb pdb:6DMI|rcsb pdb:6E3Z|rcsb pdb:6EJ2|rcsb pdb:6EJ3|rcsb pdb:6EQM|rcsb pdb:6FGY|rcsb pdb:6JSE|rcsb pdb:6JSF|rcsb pdb:6JSG|rcsb pdb:6JSN|rcsb pdb:6JT3|rcsb pdb:6JT4|rcsb pdb:6NV7|rcsb pdb:6NV9|rcsb pdb:6NW3|rcsb pdb:6OD6|rcsb pdb:6PZ4|rcsb pdb:6UVP|rcsb pdb:6UVV|rcsb pdb:6UVY|rcsb pdb:6UWP|rcsb pdb:6UWV|rcsb pdb:6WNY|rcsb pdb:7B1E|rcsb pdb:7B1P|rcsb pdb:7B1Q|rcsb pdb:7D2V|rcsb pdb:7D2X|rcsb pdb:7D5A|rcsb pdb:7DCZ|reactome:R-HSA-977225	-	-	crc64:377CE4C824ACEF05	figure legend:f2a|comment:"To corroborate these findings in an in vivo context, we performed  coimmunoprecipitation experiments with endogenous flotillins and BACE1 (Figure  2A). For this, we used polyclonal flotillin-2 antibodies since they result in a  quantitative precipitation of the pool of flotillin-1/flotillin-2 complexes in the cells.  BACE1 was found to be immunoprecipitated with endogenous flotillin-2, verifying the  relevance of the pulldown data."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-hela)|taxid:9606(Homo sapiens epitheloid cervix carcinoma cells)	-	2014/02/21	2017/06/28	rogid:P9htlLzTYk+eDKKesqCEPe/1BJA9606	rogid:3oEbxDI/Eu5LwK/3QKtUhS41n3o9606	intact-crc:59F69E500D2B3093|rigid:A6i7Uc8ZcxcpW2mbzCpZ3yLUu0k	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:O75955	uniprotkb:P56817	intact:EBI-603643|ensembl:ENSP00000365569|ensembl:ENSP00000372873|ensembl:ENSP00000373056|ensembl:ENSP00000388861|ensembl:ENSP00000391438|uniprotkb:Q969J8|uniprotkb:Q9UHW1|uniprotkb:Q9UNV8|uniprotkb:B4DVY7	intact:EBI-2433139|uniprotkb:B0YIU9|uniprotkb:Q9BYB9|uniprotkb:Q9BYC0|uniprotkb:Q9BYC1|uniprotkb:Q9UJT5|uniprotkb:Q9ULS1|uniprotkb:E9PE65|uniprotkb:H7BXJ9|uniprotkb:A0M8W7|ensembl:ENSP00000318585	psi-mi:flot1_human(display_long)|uniprotkb:FLOT1(gene name)|psi-mi:FLOT1(display_short)	psi-mi:bace1_human(display_long)|uniprotkb:BACE(gene name synonym)|uniprotkb:KIAA1149(gene name synonym)|uniprotkb:Beta-site amyloid precursor protein cleaving enzyme 1(gene name synonym)|uniprotkb:Membrane-associated aspartic protease 2(gene name synonym)|uniprotkb:Memapsin-2(gene name synonym)|uniprotkb:Aspartyl protease 2(gene name synonym)|uniprotkb:BACE1(gene name)|psi-mi:BACE1(display_short)	psi-mi:"MI:0813"(proximity ligation assay)	John et al. (2014)	pubmed:24612608|imex:IM-22281	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:2364"(proximity)	psi-mi:"MI:0471"(MINT)	intact:EBI-9213821|imex:IM-22281-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0001819"(positive regulation of cytokine production)|go:"GO:0001931"(uropod)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0002020"(protease binding)|go:"GO:0002090"(regulation of receptor internalization)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005769"(early endosome)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005886"(plasma membrane)|go:"GO:0005901"(caveola)|go:"GO:0001765"(membrane raft assembly)|go:"GO:0005911"(cell-cell junction)|go:"GO:0005912"(adherens junction)|go:"GO:0005925"(focal adhesion)|go:"GO:0007409"(axonogenesis)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016600"(flotillin complex)|go:"GO:0022617"(extracellular matrix disassembly)|go:"GO:0030027"(lamellipodium)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032092"(positive regulation of protein binding)|go:"GO:0032226"(positive regulation of synaptic transmission, dopaminergic)|go:"GO:0032728"(positive regulation of interferon-beta production)|go:"GO:0033227"(dsRNA transport)|go:"GO:0034116"(positive regulation of heterotypic cell-cell adhesion)|go:"GO:0034141"(positive regulation of toll-like receptor 3 signaling pathway)|go:"GO:0034451"(centriolar satellite)|go:"GO:0034976"(response to endoplasmic reticulum stress)|go:"GO:0035023"(regulation of Rho protein signal transduction)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0044291"(cell-cell contact zone)|go:"GO:0044854"(plasma membrane raft assembly)|go:"GO:0045121"(membrane raft)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048643"(positive regulation of skeletal muscle tissue development)|go:"GO:0048786"(presynaptic active zone)|go:"GO:0050821"(protein stabilization)|go:"GO:0051092"(positive regulation of NF-kappaB transcription factor activity)|go:"GO:0051580"(regulation of neurotransmitter uptake)|go:"GO:0060355"(positive regulation of cell adhesion molecule production)|go:"GO:0070062"(extracellular exosome)|go:"GO:0070528"(protein kinase C signaling)|go:"GO:0071360"(cellular response to exogenous dsRNA)|go:"GO:0072659"(protein localization to plasma membrane)|go:"GO:0098691"(dopaminergic synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|go:"GO:1901741"(positive regulation of myoblast fusion)|go:"GO:1901890"(positive regulation of cell junction assembly)|go:"GO:1903044"(protein localization to membrane raft)|go:"GO:2000049"(positive regulation of cell-cell adhesion mediated by cadherin)|interpro:IPR001107(Band 7 protein)|interpro:IPR027705|interpro:IPR036013|mint:O75955|reactome:R-HSA-5213460|reactome:R-HSA-5675482|reactome:R-HSA-8849932|reactome:R-HSA-8980692|reactome:R-HSA-9013026|reactome:R-HSA-9013106|ensembl:ENSG00000137312(gene)|ensembl:ENSG00000206379(gene)|ensembl:ENSG00000206480(gene)|ensembl:ENSG00000230143(gene)|ensembl:ENSG00000232280(gene)|ensembl:ENST00000376389(transcript)|ensembl:ENST00000383382(transcript)|ensembl:ENST00000383562(transcript)|ensembl:ENST00000436822(transcript)|ensembl:ENST00000444632(transcript)|refseq:NP_005794.1|refseq:NP_001305804.1|refseq:XP_005248837.1	refseq:NP_001193978.1|refseq:NP_036236.1|refseq:NP_620427.1|refseq:NP_620428.1|refseq:NP_620429.1|dip:DIP-41388N|ensembl:ENSG00000186318(gene)|ensembl:ENST00000313005(transcript)|go:"GO:0001540"(amyloid-beta binding)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004190"(aspartic-type endopeptidase activity)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005771"(multivesicular body)|go:"GO:0005788"(endoplasmic reticulum lumen)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006508"(proteolysis)|go:"GO:0006509"(membrane protein ectodomain proteolysis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0008233"(peptidase activity)|go:"GO:0008798"(beta-aspartyl-peptidase activity)|go:"GO:0009314"(response to radiation)|go:"GO:0009986"(cell surface)|go:"GO:0010008"(endosome membrane)|go:"GO:0010288"(response to lead ion)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016485"(protein processing)|go:"GO:0019899"(enzyme binding)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0034205"(amyloid-beta formation)|go:"GO:0042987"(amyloid precursor protein catabolic process)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043525"(positive regulation of neuron apoptotic process)|go:"GO:0045121"(membrane raft)|go:"GO:0050435"(amyloid-beta metabolic process)|go:"GO:0050966"(detection of mechanical stimulus involved in sensory perception of pain)|go:"GO:0055037"(recycling endosome)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0070931"(Golgi-associated vesicle lumen)|go:"GO:0071280"(cellular response to copper ion)|go:"GO:0071287"(cellular response to manganese ion)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:1904646"(cellular response to amyloid-beta)|go:"GO:1990000"(amyloid fibril formation)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001461(Peptidase A1)|interpro:IPR001969(Peptidase aspartic, active site)|interpro:IPR009119(Peptidase A1, beta-site APP cleaving enzyme, BACE)|interpro:IPR009120(Peptidase A1, beta-site APP cleaving enzyme 1, BACE 1)|interpro:IPR021109|interpro:IPR033121|interpro:IPR033874|mint:P56817|rcsb pdb:1FKN|rcsb pdb:1M4H|rcsb pdb:1PY1|rcsb pdb:1SGZ|rcsb pdb:1TQF|rcsb pdb:1UJJ|rcsb pdb:1UJK|rcsb pdb:1W50|rcsb pdb:1W51|rcsb pdb:1XN2|rcsb pdb:1XN3|rcsb pdb:1XS7|rcsb pdb:1YM2|rcsb pdb:1YM4|rcsb pdb:2B8L|rcsb pdb:2B8V|rcsb pdb:2F3E|rcsb pdb:2F3F|rcsb pdb:2FDP|rcsb pdb:2G94|rcsb pdb:2HIZ|rcsb pdb:2HM1|rcsb pdb:2IQG|rcsb pdb:2IRZ|rcsb pdb:2IS0|rcsb pdb:2NTR|rcsb pdb:2OAH|rcsb pdb:2OF0|rcsb pdb:2OHK|rcsb pdb:2OHL|rcsb pdb:2OHM|rcsb pdb:2OHN|rcsb pdb:2OHP|rcsb pdb:2OHQ|rcsb pdb:2OHR|rcsb pdb:2OHS|rcsb pdb:2OHT|rcsb pdb:2OHU|rcsb pdb:2P4J|rcsb pdb:2P83|rcsb pdb:2P8H|rcsb pdb:2PH6|rcsb pdb:2PH8|rcsb pdb:2Q11|rcsb pdb:2Q15|rcsb pdb:2QK5|rcsb pdb:2QMD|rcsb pdb:2QMF|rcsb pdb:2QMG|rcsb pdb:2QP8|rcsb pdb:2QU2|rcsb pdb:2QU3|rcsb pdb:2QZK|rcsb pdb:2QZL|rcsb pdb:2VA5|rcsb pdb:2VA6|rcsb pdb:2VA7|rcsb pdb:2VIE|rcsb pdb:2VIJ|rcsb pdb:2VIY|rcsb pdb:2VIZ|rcsb pdb:2VJ6|rcsb pdb:2VJ7|rcsb pdb:2VJ9|rcsb pdb:2VKM|rcsb pdb:2VNM|rcsb pdb:2VNN|rcsb pdb:2WEZ|rcsb pdb:2WF0|rcsb pdb:2WF1|rcsb pdb:2WF2|rcsb pdb:2WF3|rcsb pdb:2WF4|rcsb pdb:2WJO|rcsb pdb:2XFI|rcsb pdb:2XFJ|rcsb pdb:2XFK|rcsb pdb:2ZDZ|rcsb pdb:2ZE1|rcsb pdb:2ZHR|rcsb pdb:2ZHS|rcsb pdb:2ZHT|rcsb pdb:2ZHU|rcsb pdb:2ZHV|rcsb pdb:2ZJH|rcsb pdb:2ZJI|rcsb pdb:2ZJJ|rcsb pdb:2ZJK|rcsb pdb:2ZJL|rcsb pdb:2ZJM|rcsb pdb:2ZJN|rcsb pdb:3BRA|rcsb pdb:3BUF|rcsb pdb:3BUG|rcsb pdb:3BUH|rcsb pdb:3CIB|rcsb pdb:3CIC|rcsb pdb:3CID|rcsb pdb:3CKP|rcsb pdb:3CKR|rcsb pdb:3DM6|rcsb pdb:3DUY|rcsb pdb:3DV1|rcsb pdb:3DV5|rcsb pdb:3EXO|rcsb pdb:3FKT|rcsb pdb:3H0B|rcsb pdb:3HVG|rcsb pdb:3HW1|rcsb pdb:3I25|rcsb pdb:3IGB|rcsb pdb:3IN3|rcsb pdb:3IN4|rcsb pdb:3IND|rcsb pdb:3INE|rcsb pdb:3INF|rcsb pdb:3INH|rcsb pdb:3IVH|rcsb pdb:3IVI|rcsb pdb:3IXJ|rcsb pdb:3IXK|rcsb pdb:3K5C|rcsb pdb:3K5D|rcsb pdb:3K5F|rcsb pdb:3K5G|rcsb pdb:3KMX|rcsb pdb:3KMY|rcsb pdb:3KN0|rcsb pdb:3KYR|rcsb pdb:3L38|rcsb pdb:3L3A|rcsb pdb:3L58|rcsb pdb:3L59|rcsb pdb:3L5B|rcsb pdb:3L5C|rcsb pdb:3L5D|rcsb pdb:3L5E|rcsb pdb:3L5F|rcsb pdb:3LHG|rcsb pdb:3LNK|rcsb pdb:3LPI|rcsb pdb:3LPJ|rcsb pdb:3LPK|rcsb pdb:3MSJ|rcsb pdb:3MSK|rcsb pdb:3MSL|rcsb pdb:3N4L|rcsb pdb:3NSH|rcsb pdb:3OHF|rcsb pdb:3OHH|rcsb pdb:3OOZ|rcsb pdb:3PI5|rcsb pdb:3QBH|rcsb pdb:3QI1|rcsb pdb:3R1G|rcsb pdb:3R2F|rcsb pdb:3RSV|rcsb pdb:3RSX|rcsb pdb:3RTH|rcsb pdb:3RTM|rcsb pdb:3RTN|rcsb pdb:3RU1|rcsb pdb:3RVI|rcsb pdb:3S2O|rcsb pdb:3S7L|rcsb pdb:3S7M|rcsb pdb:3SKF|rcsb pdb:3TPJ|rcsb pdb:3TPL|rcsb pdb:3TPP|rcsb pdb:3TPR|rcsb pdb:3U6A|rcsb pdb:3UDH|rcsb pdb:3UDJ|rcsb pdb:3UDK|rcsb pdb:3UDM|rcsb pdb:3UDN|rcsb pdb:3UDP|rcsb pdb:3UDQ|rcsb pdb:3UDR|rcsb pdb:3UDY|rcsb pdb:3UFL|rcsb pdb:3UQP|rcsb pdb:3UQR|rcsb pdb:3UQU|rcsb pdb:3UQW|rcsb pdb:3UQX|rcsb pdb:3VEU|rcsb pdb:3VF3|rcsb pdb:3VG1|rcsb pdb:3VV6|rcsb pdb:3VV7|rcsb pdb:3VV8|rcsb pdb:3WB4|rcsb pdb:3WB5|rcsb pdb:3ZMG|rcsb pdb:3ZOV|rcsb pdb:4ACU|rcsb pdb:4ACX|rcsb pdb:4AZY|rcsb pdb:4B00|rcsb pdb:4B05|rcsb pdb:4B0Q|rcsb pdb:4B1C|rcsb pdb:4B1D|rcsb pdb:4B1E|rcsb pdb:4B70|rcsb pdb:4B72|rcsb pdb:4B77|rcsb pdb:4B78|rcsb pdb:4BEK|rcsb pdb:4BFD|rcsb pdb:4D83|rcsb pdb:4D85|rcsb pdb:4D88|rcsb pdb:4D89|rcsb pdb:4D8C|rcsb pdb:4DH6|rcsb pdb:4DI2|rcsb pdb:4DJU|rcsb pdb:4DJV|rcsb pdb:4DJW|rcsb pdb:4DJX|rcsb pdb:4DJY|rcsb pdb:4DPF|rcsb pdb:4DPI|rcsb pdb:4DUS|rcsb pdb:4DV9|rcsb pdb:4DVF|rcsb pdb:4EWO|rcsb pdb:4EXG|rcsb pdb:4FCO|rcsb pdb:4FGX|rcsb pdb:4FM7|rcsb pdb:4FM8|rcsb pdb:4FRI|rcsb pdb:4FRJ|rcsb pdb:4FRK|rcsb pdb:4FRS|rcsb pdb:4FS4|rcsb pdb:4FSE|rcsb pdb:4FSL|rcsb pdb:4GID|rcsb pdb:4GMI|rcsb pdb:4H1E|rcsb pdb:4H3F|rcsb pdb:4H3G|rcsb pdb:4H3I|rcsb pdb:4H3J|rcsb pdb:4HA5|rcsb pdb:4HZT|rcsb pdb:4I0D|rcsb pdb:4I0E|rcsb pdb:4I0F|rcsb pdb:4I0G|rcsb pdb:4I0H|rcsb pdb:4I0I|rcsb pdb:4I0J|rcsb pdb:4I0Z|rcsb pdb:4I10|rcsb pdb:4I11|rcsb pdb:3SKG|rcsb pdb:4I12|rcsb pdb:4I1C|rcsb pdb:4IVS|rcsb pdb:4IVT|rcsb pdb:4J0P|rcsb pdb:4J0T|rcsb pdb:4J0V|rcsb pdb:4J0Y|rcsb pdb:4J0Z|rcsb pdb:4J17|rcsb pdb:4J1C|rcsb pdb:4J1E|rcsb pdb:4J1F|rcsb pdb:4J1H|rcsb pdb:4J1I|rcsb pdb:4J1K|rcsb pdb:4JOO|rcsb pdb:4JP9|rcsb pdb:4JPC|rcsb pdb:4JPE|rcsb pdb:4K8S|rcsb pdb:4K9H|rcsb pdb:4KE0|rcsb pdb:4KE1|rcsb pdb:4L7G|rcsb pdb:4L7H|rcsb pdb:4L7J|rcsb pdb:4LC7|rcsb pdb:4LXA|rcsb pdb:4LXK|rcsb pdb:4LXM|rcsb pdb:4N00|rcsb pdb:4PZW|rcsb pdb:4PZX|rcsb pdb:4R5N|rcsb pdb:4R8Y|rcsb pdb:4R91|rcsb pdb:4R92|rcsb pdb:4R93|rcsb pdb:4R95|rcsb pdb:4RCD|rcsb pdb:4RCE|rcsb pdb:4RCF|rcsb pdb:4RRN|rcsb pdb:4RRO|rcsb pdb:4RRS|rcsb pdb:4TRW|rcsb pdb:4TRY|rcsb pdb:4TRZ|rcsb pdb:4WTU|rcsb pdb:4WY1|rcsb pdb:4WY6|rcsb pdb:4X2L|rcsb pdb:4X7I|rcsb pdb:4XKX|rcsb pdb:4XXS|rcsb pdb:4YBI|rcsb pdb:4ZPE|rcsb pdb:4ZPF|rcsb pdb:4ZPG|rcsb pdb:4ZSM|rcsb pdb:4ZSP|rcsb pdb:4ZSQ|rcsb pdb:4ZSR|rcsb pdb:5CLM|rcsb pdb:5DQC|rcsb pdb:5ENK|rcsb pdb:5ENM|rcsb pdb:5EZX|rcsb pdb:5EZZ|rcsb pdb:5F00|rcsb pdb:5F01|rcsb pdb:5HD0|rcsb pdb:5HDU|rcsb pdb:5HDV|rcsb pdb:5HDX|rcsb pdb:5HDZ|rcsb pdb:5HE4|rcsb pdb:5HE5|rcsb pdb:5HE7|rcsb pdb:5HTZ|rcsb pdb:5HU0|rcsb pdb:5HU1|rcsb pdb:5I3V|rcsb pdb:5I3W|rcsb pdb:5I3X|rcsb pdb:5I3Y|rcsb pdb:5IE1|rcsb pdb:5KQF|rcsb pdb:5KR8|rcsb pdb:5MBW|rcsb pdb:5MCO|rcsb pdb:5MCQ|rcsb pdb:5MXD|rcsb pdb:5QCO|rcsb pdb:5QCP|rcsb pdb:5QCQ|rcsb pdb:5QCR|rcsb pdb:5QCS|rcsb pdb:5QCT|rcsb pdb:5QCU|rcsb pdb:5QCV|rcsb pdb:5QCW|rcsb pdb:5QCX|rcsb pdb:5QCY|rcsb pdb:5QCZ|rcsb pdb:5QD0|rcsb pdb:5QD1|rcsb pdb:5QD2|rcsb pdb:5QD3|rcsb pdb:5QD4|rcsb pdb:5QD5|rcsb pdb:5QD6|rcsb pdb:5QD7|rcsb pdb:5QD8|rcsb pdb:5QD9|rcsb pdb:5QDA|rcsb pdb:5QDB|rcsb pdb:5QDC|rcsb pdb:5QDD|rcsb pdb:5T1U|rcsb pdb:5T1W|rcsb pdb:5TOL|rcsb pdb:5UYU|rcsb pdb:5V0N|rcsb pdb:5YGX|rcsb pdb:5YGY|rcsb pdb:6BFD|rcsb pdb:6BFE|rcsb pdb:6BFW|rcsb pdb:6BFX|rcsb pdb:6C2I|rcsb pdb:6DHC|rcsb pdb:6DMI|rcsb pdb:6E3Z|rcsb pdb:6EJ2|rcsb pdb:6EJ3|rcsb pdb:6EQM|rcsb pdb:6FGY|rcsb pdb:6JSE|rcsb pdb:6JSF|rcsb pdb:6JSG|rcsb pdb:6JSN|rcsb pdb:6JT3|rcsb pdb:6JT4|rcsb pdb:6NV7|rcsb pdb:6NV9|rcsb pdb:6NW3|rcsb pdb:6OD6|rcsb pdb:6PZ4|rcsb pdb:6UVP|rcsb pdb:6UVV|rcsb pdb:6UVY|rcsb pdb:6UWP|rcsb pdb:6UWV|rcsb pdb:6WNY|rcsb pdb:7B1E|rcsb pdb:7B1P|rcsb pdb:7B1Q|rcsb pdb:7D2V|rcsb pdb:7D2X|rcsb pdb:7D5A|rcsb pdb:7DCZ|reactome:R-HSA-977225	-	-	crc64:377CE4C824ACEF05	figure legend:f2b|comment:"To dissect the role of flotillins in BACE1 binding in more detail and to test if the  interaction is a direct one, we performed direct pulldown assays using bacterially  expressed, purified proteins (Figure 1D). Purified flotillin-1 again strongly bound to  the WT BACE1-GST tail, whereas no binding was observed with the LLAA tail. Only  a very weak binding that was close to the GST background level was observed with  purified flotillin-2. Taken together, these data show that flotillin-1 directly interacts  with the cytoplasmic tail of BACE1 and that this interaction is mediated by the well  known dileucine sorting motif in BACE1.  To corroborate these findings in an in vivo context, we performed  coimmunoprecipitation experiments with endogenous flotillins and BACE1 (Figure  2A). For this, we used polyclonal flotillin-2 antibodies since they result in a  quantitative precipitation of the pool of flotillin-1/flotillin-2 complexes in the cells.  BACE1 was found to be immunoprecipitated with endogenous flotillin-2, verifying the  relevance of the pulldown data. Since the signals observed in the coprecipitation  experiment were very weak, we used the Proximity Ligation Assay (PLA) to verify the  interaction of endogenous BACE1 and flotillins. PLA is based on the use of specific  antibodies and can detect two proteins that are at very close vicinity (less than 40  nm) in cells and is generally used to study interaction of proteins [38-40]. Interacting  proteins are visualized as fluorescent signals whose localization in the cells can also  be assessed, giving information on the compartment in which the interaction takes  place. However, the signal intensities as such do not correlate with the strength or  quantity of the interaction. Quantification of the signals is done by counting the  amount of fluorescent signals per cells and comparing these to negative controls  (performed without the first antibody). PLA analysis of endogenous flotillin-1 or  flotillin-2 and BACE1 showed that the fluorescent dots that arise upon interaction of  these proteins were mainly detected in the perinuclear region of the cells, with  few dots residing in the cell periphery (Fig. 2B)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-hela)|taxid:9606(Homo sapiens epitheloid cervix carcinoma cells)	-	2014/02/21	2017/06/28	rogid:wRw9QYIRNJb0ZQtwjm/D+ydci8k9606	rogid:3oEbxDI/Eu5LwK/3QKtUhS41n3o9606	intact-crc:A7500368AEF069F7|rigid:yyJ5th5yUpjOzNmQE3U1kfsfw5I	false	-	-	-	-	psi-mi:"MI:0422"(immunostaining)	psi-mi:"MI:0422"(immunostaining)
uniprotkb:Q14254	uniprotkb:P56817	intact:EBI-348613|ensembl:ENSP00000378368	intact:EBI-2433139|uniprotkb:B0YIU9|uniprotkb:Q9BYB9|uniprotkb:Q9BYC0|uniprotkb:Q9BYC1|uniprotkb:Q9UJT5|uniprotkb:Q9ULS1|uniprotkb:E9PE65|uniprotkb:H7BXJ9|uniprotkb:A0M8W7|ensembl:ENSP00000318585	psi-mi:flot2_human(display_long)|uniprotkb:FLOT2(gene name)|psi-mi:FLOT2(display_short)|uniprotkb:ESA1(gene name synonym)|uniprotkb:M17S1(gene name synonym)|uniprotkb:Epidermal surface antigen(gene name synonym)|uniprotkb:Membrane component chromosome 17 surface marker 1(gene name synonym)	psi-mi:bace1_human(display_long)|uniprotkb:BACE(gene name synonym)|uniprotkb:KIAA1149(gene name synonym)|uniprotkb:Beta-site amyloid precursor protein cleaving enzyme 1(gene name synonym)|uniprotkb:Membrane-associated aspartic protease 2(gene name synonym)|uniprotkb:Memapsin-2(gene name synonym)|uniprotkb:Aspartyl protease 2(gene name synonym)|uniprotkb:BACE1(gene name)|psi-mi:BACE1(display_short)	psi-mi:"MI:0813"(proximity ligation assay)	John et al. (2014)	pubmed:24612608|imex:IM-22281	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:2364"(proximity)	psi-mi:"MI:0471"(MINT)	intact:EBI-9213813|imex:IM-22281-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_004466.2|ensembl:ENSG00000132589(gene)|go:"GO:0008544"(epidermis development)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0016020"(membrane)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016600"(flotillin complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031982"(vesicle)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0044291"(cell-cell contact zone)|go:"GO:0044860"(protein localization to plasma membrane raft)|go:"GO:0045661"(regulation of myoblast differentiation)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050821"(protein stabilization)|go:"GO:0051092"(positive regulation of NF-kappaB transcription factor activity)|go:"GO:0070062"(extracellular exosome)|go:"GO:0072659"(protein localization to plasma membrane)|go:"GO:0098937"(anterograde dendritic transport)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099029"(anchored component of presynaptic active zone membrane)|ensembl:ENST00000394908(transcript)|go:"GO:0001765"(membrane raft assembly)|go:"GO:0001931"(uropod)|go:"GO:0002020"(protease binding)|go:"GO:0002080"(acrosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0005901"(caveola)|go:"GO:0005912"(adherens junction)|go:"GO:0005925"(focal adhesion)|go:"GO:0007155"(cell adhesion)|go:"GO:0099072"(regulation of postsynaptic membrane neurotransmitter receptor levels)|go:"GO:1902992"(negative regulation of amyloid precursor protein catabolic process)|go:"GO:1903905"(positive regulation of establishment of T cell polarity)|interpro:IPR001107(Band 7 protein)|interpro:IPR027705|interpro:IPR031905|interpro:IPR036013|mint:Q14254|reactome:R-HSA-5213460|reactome:R-HSA-5675482|reactome:R-HSA-8849932|reactome:R-HSA-8980692|reactome:R-HSA-9013026|reactome:R-HSA-9013106|reactome:R-HSA-9696264|reactome:R-HSA-9696273|refseq:XP_016879883.1|refseq:XP_016879884.1	refseq:NP_001193978.1|refseq:NP_036236.1|refseq:NP_620427.1|refseq:NP_620428.1|refseq:NP_620429.1|dip:DIP-41388N|ensembl:ENSG00000186318(gene)|ensembl:ENST00000313005(transcript)|go:"GO:0001540"(amyloid-beta binding)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004190"(aspartic-type endopeptidase activity)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005771"(multivesicular body)|go:"GO:0005788"(endoplasmic reticulum lumen)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006508"(proteolysis)|go:"GO:0006509"(membrane protein ectodomain proteolysis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0008233"(peptidase activity)|go:"GO:0008798"(beta-aspartyl-peptidase activity)|go:"GO:0009314"(response to radiation)|go:"GO:0009986"(cell surface)|go:"GO:0010008"(endosome membrane)|go:"GO:0010288"(response to lead ion)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016485"(protein processing)|go:"GO:0019899"(enzyme binding)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0034205"(amyloid-beta formation)|go:"GO:0042987"(amyloid precursor protein catabolic process)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043525"(positive regulation of neuron apoptotic process)|go:"GO:0045121"(membrane raft)|go:"GO:0050435"(amyloid-beta metabolic process)|go:"GO:0050966"(detection of mechanical stimulus involved in sensory perception of pain)|go:"GO:0055037"(recycling endosome)|go:"GO:0060134"(prepulse inhibition)|go:"GO:0070931"(Golgi-associated vesicle lumen)|go:"GO:0071280"(cellular response to copper ion)|go:"GO:0071287"(cellular response to manganese ion)|go:"GO:0098686"(hippocampal mossy fiber to CA3 synapse)|go:"GO:1904646"(cellular response to amyloid-beta)|go:"GO:1990000"(amyloid fibril formation)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001461(Peptidase A1)|interpro:IPR001969(Peptidase aspartic, active site)|interpro:IPR009119(Peptidase A1, beta-site APP cleaving enzyme, BACE)|interpro:IPR009120(Peptidase A1, beta-site APP cleaving enzyme 1, BACE 1)|interpro:IPR021109|interpro:IPR033121|interpro:IPR033874|mint:P56817|rcsb pdb:1FKN|rcsb pdb:1M4H|rcsb pdb:1PY1|rcsb pdb:1SGZ|rcsb pdb:1TQF|rcsb pdb:1UJJ|rcsb pdb:1UJK|rcsb pdb:1W50|rcsb pdb:1W51|rcsb pdb:1XN2|rcsb pdb:1XN3|rcsb pdb:1XS7|rcsb pdb:1YM2|rcsb pdb:1YM4|rcsb pdb:2B8L|rcsb pdb:2B8V|rcsb pdb:2F3E|rcsb pdb:2F3F|rcsb pdb:2FDP|rcsb pdb:2G94|rcsb pdb:2HIZ|rcsb pdb:2HM1|rcsb pdb:2IQG|rcsb pdb:2IRZ|rcsb pdb:2IS0|rcsb pdb:2NTR|rcsb pdb:2OAH|rcsb pdb:2OF0|rcsb pdb:2OHK|rcsb pdb:2OHL|rcsb pdb:2OHM|rcsb pdb:2OHN|rcsb pdb:2OHP|rcsb pdb:2OHQ|rcsb pdb:2OHR|rcsb pdb:2OHS|rcsb pdb:2OHT|rcsb pdb:2OHU|rcsb pdb:2P4J|rcsb pdb:2P83|rcsb pdb:2P8H|rcsb pdb:2PH6|rcsb pdb:2PH8|rcsb pdb:2Q11|rcsb pdb:2Q15|rcsb pdb:2QK5|rcsb pdb:2QMD|rcsb pdb:2QMF|rcsb pdb:2QMG|rcsb pdb:2QP8|rcsb pdb:2QU2|rcsb pdb:2QU3|rcsb pdb:2QZK|rcsb pdb:2QZL|rcsb pdb:2VA5|rcsb pdb:2VA6|rcsb pdb:2VA7|rcsb pdb:2VIE|rcsb pdb:2VIJ|rcsb pdb:2VIY|rcsb pdb:2VIZ|rcsb pdb:2VJ6|rcsb pdb:2VJ7|rcsb pdb:2VJ9|rcsb pdb:2VKM|rcsb pdb:2VNM|rcsb pdb:2VNN|rcsb pdb:2WEZ|rcsb pdb:2WF0|rcsb pdb:2WF1|rcsb pdb:2WF2|rcsb pdb:2WF3|rcsb pdb:2WF4|rcsb pdb:2WJO|rcsb pdb:2XFI|rcsb pdb:2XFJ|rcsb pdb:2XFK|rcsb pdb:2ZDZ|rcsb pdb:2ZE1|rcsb pdb:2ZHR|rcsb pdb:2ZHS|rcsb pdb:2ZHT|rcsb pdb:2ZHU|rcsb pdb:2ZHV|rcsb pdb:2ZJH|rcsb pdb:2ZJI|rcsb pdb:2ZJJ|rcsb pdb:2ZJK|rcsb pdb:2ZJL|rcsb pdb:2ZJM|rcsb pdb:2ZJN|rcsb pdb:3BRA|rcsb pdb:3BUF|rcsb pdb:3BUG|rcsb pdb:3BUH|rcsb pdb:3CIB|rcsb pdb:3CIC|rcsb pdb:3CID|rcsb pdb:3CKP|rcsb pdb:3CKR|rcsb pdb:3DM6|rcsb pdb:3DUY|rcsb pdb:3DV1|rcsb pdb:3DV5|rcsb pdb:3EXO|rcsb pdb:3FKT|rcsb pdb:3H0B|rcsb pdb:3HVG|rcsb pdb:3HW1|rcsb pdb:3I25|rcsb pdb:3IGB|rcsb pdb:3IN3|rcsb pdb:3IN4|rcsb pdb:3IND|rcsb pdb:3INE|rcsb pdb:3INF|rcsb pdb:3INH|rcsb pdb:3IVH|rcsb pdb:3IVI|rcsb pdb:3IXJ|rcsb pdb:3IXK|rcsb pdb:3K5C|rcsb pdb:3K5D|rcsb pdb:3K5F|rcsb pdb:3K5G|rcsb pdb:3KMX|rcsb pdb:3KMY|rcsb pdb:3KN0|rcsb pdb:3KYR|rcsb pdb:3L38|rcsb pdb:3L3A|rcsb pdb:3L58|rcsb pdb:3L59|rcsb pdb:3L5B|rcsb pdb:3L5C|rcsb pdb:3L5D|rcsb pdb:3L5E|rcsb pdb:3L5F|rcsb pdb:3LHG|rcsb pdb:3LNK|rcsb pdb:3LPI|rcsb pdb:3LPJ|rcsb pdb:3LPK|rcsb pdb:3MSJ|rcsb pdb:3MSK|rcsb pdb:3MSL|rcsb pdb:3N4L|rcsb pdb:3NSH|rcsb pdb:3OHF|rcsb pdb:3OHH|rcsb pdb:3OOZ|rcsb pdb:3PI5|rcsb pdb:3QBH|rcsb pdb:3QI1|rcsb pdb:3R1G|rcsb pdb:3R2F|rcsb pdb:3RSV|rcsb pdb:3RSX|rcsb pdb:3RTH|rcsb pdb:3RTM|rcsb pdb:3RTN|rcsb pdb:3RU1|rcsb pdb:3RVI|rcsb pdb:3S2O|rcsb pdb:3S7L|rcsb pdb:3S7M|rcsb pdb:3SKF|rcsb pdb:3TPJ|rcsb pdb:3TPL|rcsb pdb:3TPP|rcsb pdb:3TPR|rcsb pdb:3U6A|rcsb pdb:3UDH|rcsb pdb:3UDJ|rcsb pdb:3UDK|rcsb pdb:3UDM|rcsb pdb:3UDN|rcsb pdb:3UDP|rcsb pdb:3UDQ|rcsb pdb:3UDR|rcsb pdb:3UDY|rcsb pdb:3UFL|rcsb pdb:3UQP|rcsb pdb:3UQR|rcsb pdb:3UQU|rcsb pdb:3UQW|rcsb pdb:3UQX|rcsb pdb:3VEU|rcsb pdb:3VF3|rcsb pdb:3VG1|rcsb pdb:3VV6|rcsb pdb:3VV7|rcsb pdb:3VV8|rcsb pdb:3WB4|rcsb pdb:3WB5|rcsb pdb:3ZMG|rcsb pdb:3ZOV|rcsb pdb:4ACU|rcsb pdb:4ACX|rcsb pdb:4AZY|rcsb pdb:4B00|rcsb pdb:4B05|rcsb pdb:4B0Q|rcsb pdb:4B1C|rcsb pdb:4B1D|rcsb pdb:4B1E|rcsb pdb:4B70|rcsb pdb:4B72|rcsb pdb:4B77|rcsb pdb:4B78|rcsb pdb:4BEK|rcsb pdb:4BFD|rcsb pdb:4D83|rcsb pdb:4D85|rcsb pdb:4D88|rcsb pdb:4D89|rcsb pdb:4D8C|rcsb pdb:4DH6|rcsb pdb:4DI2|rcsb pdb:4DJU|rcsb pdb:4DJV|rcsb pdb:4DJW|rcsb pdb:4DJX|rcsb pdb:4DJY|rcsb pdb:4DPF|rcsb pdb:4DPI|rcsb pdb:4DUS|rcsb pdb:4DV9|rcsb pdb:4DVF|rcsb pdb:4EWO|rcsb pdb:4EXG|rcsb pdb:4FCO|rcsb pdb:4FGX|rcsb pdb:4FM7|rcsb pdb:4FM8|rcsb pdb:4FRI|rcsb pdb:4FRJ|rcsb pdb:4FRK|rcsb pdb:4FRS|rcsb pdb:4FS4|rcsb pdb:4FSE|rcsb pdb:4FSL|rcsb pdb:4GID|rcsb pdb:4GMI|rcsb pdb:4H1E|rcsb pdb:4H3F|rcsb pdb:4H3G|rcsb pdb:4H3I|rcsb pdb:4H3J|rcsb pdb:4HA5|rcsb pdb:4HZT|rcsb pdb:4I0D|rcsb pdb:4I0E|rcsb pdb:4I0F|rcsb pdb:4I0G|rcsb pdb:4I0H|rcsb pdb:4I0I|rcsb pdb:4I0J|rcsb pdb:4I0Z|rcsb pdb:4I10|rcsb pdb:4I11|rcsb pdb:3SKG|rcsb pdb:4I12|rcsb pdb:4I1C|rcsb pdb:4IVS|rcsb pdb:4IVT|rcsb pdb:4J0P|rcsb pdb:4J0T|rcsb pdb:4J0V|rcsb pdb:4J0Y|rcsb pdb:4J0Z|rcsb pdb:4J17|rcsb pdb:4J1C|rcsb pdb:4J1E|rcsb pdb:4J1F|rcsb pdb:4J1H|rcsb pdb:4J1I|rcsb pdb:4J1K|rcsb pdb:4JOO|rcsb pdb:4JP9|rcsb pdb:4JPC|rcsb pdb:4JPE|rcsb pdb:4K8S|rcsb pdb:4K9H|rcsb pdb:4KE0|rcsb pdb:4KE1|rcsb pdb:4L7G|rcsb pdb:4L7H|rcsb pdb:4L7J|rcsb pdb:4LC7|rcsb pdb:4LXA|rcsb pdb:4LXK|rcsb pdb:4LXM|rcsb pdb:4N00|rcsb pdb:4PZW|rcsb pdb:4PZX|rcsb pdb:4R5N|rcsb pdb:4R8Y|rcsb pdb:4R91|rcsb pdb:4R92|rcsb pdb:4R93|rcsb pdb:4R95|rcsb pdb:4RCD|rcsb pdb:4RCE|rcsb pdb:4RCF|rcsb pdb:4RRN|rcsb pdb:4RRO|rcsb pdb:4RRS|rcsb pdb:4TRW|rcsb pdb:4TRY|rcsb pdb:4TRZ|rcsb pdb:4WTU|rcsb pdb:4WY1|rcsb pdb:4WY6|rcsb pdb:4X2L|rcsb pdb:4X7I|rcsb pdb:4XKX|rcsb pdb:4XXS|rcsb pdb:4YBI|rcsb pdb:4ZPE|rcsb pdb:4ZPF|rcsb pdb:4ZPG|rcsb pdb:4ZSM|rcsb pdb:4ZSP|rcsb pdb:4ZSQ|rcsb pdb:4ZSR|rcsb pdb:5CLM|rcsb pdb:5DQC|rcsb pdb:5ENK|rcsb pdb:5ENM|rcsb pdb:5EZX|rcsb pdb:5EZZ|rcsb pdb:5F00|rcsb pdb:5F01|rcsb pdb:5HD0|rcsb pdb:5HDU|rcsb pdb:5HDV|rcsb pdb:5HDX|rcsb pdb:5HDZ|rcsb pdb:5HE4|rcsb pdb:5HE5|rcsb pdb:5HE7|rcsb pdb:5HTZ|rcsb pdb:5HU0|rcsb pdb:5HU1|rcsb pdb:5I3V|rcsb pdb:5I3W|rcsb pdb:5I3X|rcsb pdb:5I3Y|rcsb pdb:5IE1|rcsb pdb:5KQF|rcsb pdb:5KR8|rcsb pdb:5MBW|rcsb pdb:5MCO|rcsb pdb:5MCQ|rcsb pdb:5MXD|rcsb pdb:5QCO|rcsb pdb:5QCP|rcsb pdb:5QCQ|rcsb pdb:5QCR|rcsb pdb:5QCS|rcsb pdb:5QCT|rcsb pdb:5QCU|rcsb pdb:5QCV|rcsb pdb:5QCW|rcsb pdb:5QCX|rcsb pdb:5QCY|rcsb pdb:5QCZ|rcsb pdb:5QD0|rcsb pdb:5QD1|rcsb pdb:5QD2|rcsb pdb:5QD3|rcsb pdb:5QD4|rcsb pdb:5QD5|rcsb pdb:5QD6|rcsb pdb:5QD7|rcsb pdb:5QD8|rcsb pdb:5QD9|rcsb pdb:5QDA|rcsb pdb:5QDB|rcsb pdb:5QDC|rcsb pdb:5QDD|rcsb pdb:5T1U|rcsb pdb:5T1W|rcsb pdb:5TOL|rcsb pdb:5UYU|rcsb pdb:5V0N|rcsb pdb:5YGX|rcsb pdb:5YGY|rcsb pdb:6BFD|rcsb pdb:6BFE|rcsb pdb:6BFW|rcsb pdb:6BFX|rcsb pdb:6C2I|rcsb pdb:6DHC|rcsb pdb:6DMI|rcsb pdb:6E3Z|rcsb pdb:6EJ2|rcsb pdb:6EJ3|rcsb pdb:6EQM|rcsb pdb:6FGY|rcsb pdb:6JSE|rcsb pdb:6JSF|rcsb pdb:6JSG|rcsb pdb:6JSN|rcsb pdb:6JT3|rcsb pdb:6JT4|rcsb pdb:6NV7|rcsb pdb:6NV9|rcsb pdb:6NW3|rcsb pdb:6OD6|rcsb pdb:6PZ4|rcsb pdb:6UVP|rcsb pdb:6UVV|rcsb pdb:6UVY|rcsb pdb:6UWP|rcsb pdb:6UWV|rcsb pdb:6WNY|rcsb pdb:7B1E|rcsb pdb:7B1P|rcsb pdb:7B1Q|rcsb pdb:7D2V|rcsb pdb:7D2X|rcsb pdb:7D5A|rcsb pdb:7DCZ|reactome:R-HSA-977225	-	-	crc64:377CE4C824ACEF05	figure legend:f2b|comment:"To dissect the role of flotillins in BACE1 binding in more detail and to test if the  interaction is a direct one, we performed direct pulldown assays using bacterially  expressed, purified proteins (Figure 1D). Purified flotillin-1 again strongly bound to  the WT BACE1-GST tail, whereas no binding was observed with the LLAA tail. Only  a very weak binding that was close to the GST background level was observed with  purified flotillin-2. Taken together, these data show that flotillin-1 directly interacts  with the cytoplasmic tail of BACE1 and that this interaction is mediated by the well  known dileucine sorting motif in BACE1.  To corroborate these findings in an in vivo context, we performed  coimmunoprecipitation experiments with endogenous flotillins and BACE1 (Figure  2A). For this, we used polyclonal flotillin-2 antibodies since they result in a  quantitative precipitation of the pool of flotillin-1/flotillin-2 complexes in the cells.  BACE1 was found to be immunoprecipitated with endogenous flotillin-2, verifying the  relevance of the pulldown data. Since the signals observed in the coprecipitation  experiment were very weak, we used the Proximity Ligation Assay (PLA) to verify the  interaction of endogenous BACE1 and flotillins. PLA is based on the use of specific  antibodies and can detect two proteins that are at very close vicinity (less than 40  nm) in cells and is generally used to study interaction of proteins [38-40]. Interacting  proteins are visualized as fluorescent signals whose localization in the cells can also  be assessed, giving information on the compartment in which the interaction takes  place. However, the signal intensities as such do not correlate with the strength or  quantity of the interaction. Quantification of the signals is done by counting the  amount of fluorescent signals per cells and comparing these to negative controls  (performed without the first antibody). PLA analysis of endogenous flotillin-1 or  flotillin-2 and BACE1 showed that the fluorescent dots that arise upon interaction of  these proteins were mainly detected in the perinuclear region of the cells, with  few dots residing in the cell periphery (Fig. 2B)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-hela)|taxid:9606(Homo sapiens epitheloid cervix carcinoma cells)	-	2014/02/21	2017/06/28	rogid:P9htlLzTYk+eDKKesqCEPe/1BJA9606	rogid:3oEbxDI/Eu5LwK/3QKtUhS41n3o9606	intact-crc:9B717045BE761A40|rigid:A6i7Uc8ZcxcpW2mbzCpZ3yLUu0k	false	-	-	-	-	psi-mi:"MI:0422"(immunostaining)	psi-mi:"MI:0422"(immunostaining)