#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:Q9Z2I6 uniprotkb:P0DPI0 intact:EBI-8178859|intact:MINT-1795475|ensembl:ENSRNOP00000024459 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 psi-mi:sv2c_rat(display_long)|uniprotkb:Sv2c(gene name)|psi-mi:Sv2c(display_short) psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:"MI:0096"(pull down) - (2014) imex:IM-22230|pubmed:24583011 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:1491(clobo)|taxid:1491(Clostridium botulinum) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9118895|imex:IM-22230-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:5JMC|refseq:XP_017446285.1|refseq:NP_113781.1|rgd:619718|ensembl:ENSRNOG00000018094(gene)|ensembl:ENSRNOT00000024459(transcript)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259 rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) - comment:mint|function:Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles|function:Receptor for the botulinium neurotoxin type A/BOTA comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" figure legend:f2|comment:"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:ndr8X1sl1ne8Knc4Hcpv24uqsHA10116 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 intact-crc:72AE68BFB4FA030C|rigid:WvFS7Cs/7eb772+sTlq0INsXCPc false binding-associated region:454-579|glutathione s tranferase tag:?-? mutation decreasing interaction:1131-1131|his tag:?-? - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0DPI0 uniprotkb:Q02563 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 intact:EBI-466194|uniprotkb:Q58DZ8|ensembl:ENSRNOP00000028760 psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:sv2a_rat(display_long)|uniprotkb:Sv2a(gene name)|psi-mi:Sv2a(display_short)|uniprotkb:Sv2(gene name synonym) psi-mi:"MI:0096"(pull down) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9118780|imex:IM-22230-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) rgd:619715|refseq:NP_476558.2|refseq:XP_006233014.1|ensembl:ENSRNOT00000028760(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0014052"(regulation of gamma-aminobutyric acid secretion)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016082"(synaptic vesicle priming)|go:"GO:0019901"(protein kinase binding)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030425"(dendrite)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0045202"(synapse)|go:"GO:0048786"(presynaptic active zone)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259|mint:Q02563|ensembl:ENSRNOG00000021182(gene) - comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" - figure legend:sf1|comment:"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 rogid:a7TA6gTNT5eWAIVLxjT1dgUQzn410116 intact-crc:5C399ACB6CE05CF3|rigid:XRONUvQYt++Owq/3bScBAKn3MRM false binding-associated region:449-1296|tandem tag:?-? binding-associated region:468-594|glutathione s tranferase tag:?-? - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) uniprotkb:P0DPI0 uniprotkb:Q9Z2I6 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 intact:EBI-8178859|intact:MINT-1795475|ensembl:ENSRNOP00000024459 psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:sv2c_rat(display_long)|uniprotkb:Sv2c(gene name)|psi-mi:Sv2c(display_short) psi-mi:"MI:0096"(pull down) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9119010|imex:IM-22230-8 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) rcsb pdb:5JMC|refseq:XP_017446285.1|refseq:NP_113781.1|rgd:619718|ensembl:ENSRNOG00000018094(gene)|ensembl:ENSRNOT00000024459(transcript)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259 - comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" comment:mint|function:Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles|function:Receptor for the botulinium neurotoxin type A/BOTA figure legend:f2|comment:"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 rogid:ndr8X1sl1ne8Knc4Hcpv24uqsHA10116 intact-crc:1B40EBB5787E57C8|rigid:WvFS7Cs/7eb772+sTlq0INsXCPc false mutation decreasing interaction:1064-1064|his tag:?-? glutathione s tranferase tag:?-?|binding-associated region:454-579 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0DPI0 uniprotkb:Q9Z2I6 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 intact:EBI-8178859|intact:MINT-1795475|ensembl:ENSRNOP00000024459 psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:sv2c_rat(display_long)|uniprotkb:Sv2c(gene name)|psi-mi:Sv2c(display_short) psi-mi:"MI:0096"(pull down) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9119075|imex:IM-22230-11 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) rcsb pdb:5JMC|refseq:XP_017446285.1|refseq:NP_113781.1|rgd:619718|ensembl:ENSRNOG00000018094(gene)|ensembl:ENSRNOT00000024459(transcript)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259 - comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" comment:mint|function:Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles|function:Receptor for the botulinium neurotoxin type A/BOTA figure legend:f2|comment:"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 rogid:ndr8X1sl1ne8Knc4Hcpv24uqsHA10116 intact-crc:CE4899655E2EDC25|rigid:WvFS7Cs/7eb772+sTlq0INsXCPc false mutation decreasing interaction:1287-1287|his tag:?-? binding-associated region:454-579|glutathione s tranferase tag:?-? - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0DPI0 uniprotkb:Q9Z2I6 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 intact:EBI-8178859|intact:MINT-1795475|ensembl:ENSRNOP00000024459 psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:sv2c_rat(display_long)|uniprotkb:Sv2c(gene name)|psi-mi:Sv2c(display_short) psi-mi:"MI:0096"(pull down) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9119090|imex:IM-22230-12 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) rcsb pdb:5JMC|refseq:XP_017446285.1|refseq:NP_113781.1|rgd:619718|ensembl:ENSRNOG00000018094(gene)|ensembl:ENSRNOT00000024459(transcript)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259 - comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" comment:mint|function:Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles|function:Receptor for the botulinium neurotoxin type A/BOTA figure legend:f|comment:"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 rogid:ndr8X1sl1ne8Knc4Hcpv24uqsHA10116 intact-crc:43AD74238E95B4F0|rigid:WvFS7Cs/7eb772+sTlq0INsXCPc false mutation decreasing interaction:1292-1292|his tag:?-? binding-associated region:454-579|glutathione s tranferase tag:?-? - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0DPI0 uniprotkb:Q9Z2I6 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 intact:EBI-8178859|intact:MINT-1795475|ensembl:ENSRNOP00000024459 psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:sv2c_rat(display_long)|uniprotkb:Sv2c(gene name)|psi-mi:Sv2c(display_short) psi-mi:"MI:0096"(pull down) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9118960|imex:IM-22230-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) rcsb pdb:5JMC|refseq:XP_017446285.1|refseq:NP_113781.1|rgd:619718|ensembl:ENSRNOG00000018094(gene)|ensembl:ENSRNOT00000024459(transcript)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259 - comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" comment:mint|function:Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles|function:Receptor for the botulinium neurotoxin type A/BOTA figure legend:f2|comment:"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 rogid:ndr8X1sl1ne8Knc4Hcpv24uqsHA10116 intact-crc:CB2E4F39A4488D4A|rigid:WvFS7Cs/7eb772+sTlq0INsXCPc false his tag:?-?|mutation decreasing interaction:1188-1188 glutathione s tranferase tag:?-?|binding-associated region:454-579 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:Q9Z2I6 uniprotkb:P0DPI0 intact:EBI-8178859|intact:MINT-1795475|ensembl:ENSRNOP00000024459 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 psi-mi:sv2c_rat(display_long)|uniprotkb:Sv2c(gene name)|psi-mi:Sv2c(display_short) psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:"MI:0096"(pull down) - (2014) imex:IM-22230|pubmed:24583011 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:1491(clobo)|taxid:1491(Clostridium botulinum) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9118756|imex:IM-22230-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:5JMC|refseq:XP_017446285.1|refseq:NP_113781.1|rgd:619718|ensembl:ENSRNOG00000018094(gene)|ensembl:ENSRNOT00000024459(transcript)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259 rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) - comment:mint|function:Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles|function:Receptor for the botulinium neurotoxin type A/BOTA comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" figure legend:f1a|comment:"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:ndr8X1sl1ne8Knc4Hcpv24uqsHA10116 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 intact-crc:5C39859C0D9B5C92|rigid:WvFS7Cs/7eb772+sTlq0INsXCPc false glutathione s tranferase tag:?-?|binding-associated region:454-579 his tag:?-? - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) uniprotkb:P0DPI0 uniprotkb:Q9Z2I6 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 intact:EBI-8178859|intact:MINT-1795475|ensembl:ENSRNOP00000024459 psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:sv2c_rat(display_long)|uniprotkb:Sv2c(gene name)|psi-mi:Sv2c(display_short) psi-mi:"MI:0096"(pull down) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9118993|imex:IM-22230-7 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) rcsb pdb:5JMC|refseq:XP_017446285.1|refseq:NP_113781.1|rgd:619718|ensembl:ENSRNOG00000018094(gene)|ensembl:ENSRNOT00000024459(transcript)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259 - comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" comment:mint|function:Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles|function:Receptor for the botulinium neurotoxin type A/BOTA figure legend:f2|comment:"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 rogid:ndr8X1sl1ne8Knc4Hcpv24uqsHA10116 intact-crc:E50BFE5A862A4A5A|rigid:WvFS7Cs/7eb772+sTlq0INsXCPc false his tag:?-?|mutation decreasing interaction:1063-1063 binding-associated region:454-579|glutathione s tranferase tag:?-? - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0DPI0 uniprotkb:Q9Z2I6 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 intact:EBI-8178859|intact:MINT-1795475|ensembl:ENSRNOP00000024459 psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:sv2c_rat(display_long)|uniprotkb:Sv2c(gene name)|psi-mi:Sv2c(display_short) psi-mi:"MI:0096"(pull down) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9118977|imex:IM-22230-6 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) rcsb pdb:5JMC|refseq:XP_017446285.1|refseq:NP_113781.1|rgd:619718|ensembl:ENSRNOG00000018094(gene)|ensembl:ENSRNOT00000024459(transcript)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259 - comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" comment:mint|function:Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles|function:Receptor for the botulinium neurotoxin type A/BOTA figure legend:f2|comment:"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 rogid:ndr8X1sl1ne8Knc4Hcpv24uqsHA10116 intact-crc:1DCB20F5B75C2C1D|rigid:WvFS7Cs/7eb772+sTlq0INsXCPc false mutation decreasing interaction:905-905|his tag:?-? binding-associated region:454-579|glutathione s tranferase tag:?-? - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0DPI0 uniprotkb:Q9Z2I6 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 intact:EBI-8178859|intact:MINT-1795475|ensembl:ENSRNOP00000024459 psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:sv2c_rat(display_long)|uniprotkb:Sv2c(gene name)|psi-mi:Sv2c(display_short) psi-mi:"MI:0096"(pull down) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9118929|imex:IM-22230-4 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) rcsb pdb:5JMC|refseq:XP_017446285.1|refseq:NP_113781.1|rgd:619718|ensembl:ENSRNOG00000018094(gene)|ensembl:ENSRNOT00000024459(transcript)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259 - comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" comment:mint|function:Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles|function:Receptor for the botulinium neurotoxin type A/BOTA figure legend:f2|comment:"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 rogid:ndr8X1sl1ne8Knc4Hcpv24uqsHA10116 intact-crc:B93DD086F7B51F8A|rigid:WvFS7Cs/7eb772+sTlq0INsXCPc false his tag:?-?|mutation decreasing interaction:1163-1163 glutathione s tranferase tag:?-?|binding-associated region:454-579 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:Q9Z2I6 uniprotkb:P0DPI0 intact:EBI-8178859|intact:MINT-1795475|ensembl:ENSRNOP00000024459 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 psi-mi:sv2c_rat(display_long)|uniprotkb:Sv2c(gene name)|psi-mi:Sv2c(display_short) psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:"MI:0096"(pull down) - (2014) imex:IM-22230|pubmed:24583011 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:1491(clobo)|taxid:1491(Clostridium botulinum) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9119028|imex:IM-22230-9 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:5JMC|refseq:XP_017446285.1|refseq:NP_113781.1|rgd:619718|ensembl:ENSRNOG00000018094(gene)|ensembl:ENSRNOT00000024459(transcript)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259 rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) - comment:mint|function:Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles|function:Receptor for the botulinium neurotoxin type A/BOTA comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" figure legend:f2|comment:"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:ndr8X1sl1ne8Knc4Hcpv24uqsHA10116 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 intact-crc:5960684495141502|rigid:WvFS7Cs/7eb772+sTlq0INsXCPc false glutathione s tranferase tag:?-?|binding-associated region:454-579 his tag:?-?|mutation decreasing interaction:1108-1108 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:Q9Z2I6 uniprotkb:P0DPI0 intact:EBI-8178859|intact:MINT-1795475|ensembl:ENSRNOP00000024459 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 psi-mi:sv2c_rat(display_long)|uniprotkb:Sv2c(gene name)|psi-mi:Sv2c(display_short) psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:"MI:0096"(pull down) - (2014) imex:IM-22230|pubmed:24583011 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:1491(clobo)|taxid:1491(Clostridium botulinum) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-9119048|imex:IM-22230-10 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:5JMC|refseq:XP_017446285.1|refseq:NP_113781.1|rgd:619718|ensembl:ENSRNOG00000018094(gene)|ensembl:ENSRNOT00000024459(transcript)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259 rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) - comment:mint|function:Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles|function:Receptor for the botulinium neurotoxin type A/BOTA comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" figure legend:f2|comment:"This direct protein-protein interaction is clearly demonstrated in a GST pull down assay employing full-length recombinant H6tBoNTA and rat GST-rSV2C 454-579 (Fig. 1A). The weaker binding of HCA to recombinant rat GST-rSV2A 468-594 is also shown (Fig. S1) whereas binding to non-glycosylated rSV2B is hardly detectable. Well characterised neutralising monoclonal antibodies directed against different epitopes within the HC-fragment of BoNT/A [25-29] were screened for interference with the SV2C interaction"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:ndr8X1sl1ne8Knc4Hcpv24uqsHA10116 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 intact-crc:393EC6FF6D2B311D|rigid:WvFS7Cs/7eb772+sTlq0INsXCPc false glutathione s tranferase tag:?-?|binding-associated region:454-579 his tag:?-?|mutation decreasing interaction:1156-1156 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P30996 uniprotkb:Q02563 intact:EBI-9119207 intact:EBI-466194|uniprotkb:Q58DZ8|ensembl:ENSRNOP00000028760 psi-mi:bxf_clobo(display_long)|uniprotkb:botF(gene name)|psi-mi:botF(display_short)|uniprotkb:Bontoxilysin-F(gene name synonym) psi-mi:sv2a_rat(display_long)|uniprotkb:Sv2a(gene name)|psi-mi:Sv2a(display_short)|uniprotkb:Sv2(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0471"(MINT) intact:EBI-9119236|imex:IM-22230-15 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008289"(lipid binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR012500(Clostridium neurotoxin, translocation)|rcsb pdb:2A8A|rcsb pdb:2A97|reactome:R-HSA-5250981|interpro:IPR036248 rgd:619715|refseq:NP_476558.2|refseq:XP_006233014.1|ensembl:ENSRNOT00000028760(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0014052"(regulation of gamma-aminobutyric acid secretion)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016082"(synaptic vesicle priming)|go:"GO:0019901"(protein kinase binding)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030425"(dendrite)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0045202"(synapse)|go:"GO:0048786"(presynaptic active zone)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259|mint:Q02563|ensembl:ENSRNOG00000021182(gene) - - - figure legend:f4|comment:HCA mutants R1156M and V1287E showed similar affinity to SV2A and SV2B as wild-type HCA indicating subtle differences between the mode of interaction of SV2A/B and of SV2C with BoNT/A|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:VkdPeGwB+fU9RbXbftw8PkIJTLo1491 rogid:a7TA6gTNT5eWAIVLxjT1dgUQzn410116 intact-crc:67EE3852EFEB9636|rigid:RT6tuTQ9wR8CiGBXyLmJgwZu26Q false binding-associated region:437-1274|tandem tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P30996 uniprotkb:Q63564 intact:EBI-9119207 intact:EBI-9254006 psi-mi:bxf_clobo(display_long)|uniprotkb:botF(gene name)|psi-mi:botF(display_short)|uniprotkb:Bontoxilysin-F(gene name synonym) psi-mi:sv2b_rat(display_long)|uniprotkb:Sv2b(gene name)|psi-mi:Sv2b(display_short)|uniprotkb:Sv2bb(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0471"(MINT) intact:EBI-9119236|imex:IM-22230-15 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008289"(lipid binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR012500(Clostridium neurotoxin, translocation)|rcsb pdb:2A8A|rcsb pdb:2A97|reactome:R-HSA-5250981|interpro:IPR036248 interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR022308|mint:Q63564|rgd:619716|refseq:NP_476555.1|interpro:IPR036259|go:"GO:0001504"(neurotransmitter uptake)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|go:"GO:0043195"(terminal bouton) - - - figure legend:f4|comment:HCA mutants R1156M and V1287E showed similar affinity to SV2A and SV2B as wild-type HCA indicating subtle differences between the mode of interaction of SV2A/B and of SV2C with BoNT/A|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:VkdPeGwB+fU9RbXbftw8PkIJTLo1491 rogid:PnkAiRP4l+Bkf3cK1gDGTPCsT/810116 intact-crc:67EE3852EFEB9636|rigid:RT6tuTQ9wR8CiGBXyLmJgwZu26Q false binding-associated region:437-1274|tandem tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P0DPI0 uniprotkb:Q63564 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 intact:EBI-9254006 psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:sv2b_rat(display_long)|uniprotkb:Sv2b(gene name)|psi-mi:Sv2b(display_short)|uniprotkb:Sv2bb(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0471"(MINT) intact:EBI-9119119|imex:IM-22230-13 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR022308|mint:Q63564|rgd:619716|refseq:NP_476555.1|interpro:IPR036259|go:"GO:0001504"(neurotransmitter uptake)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|go:"GO:0043195"(terminal bouton) - comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" - figure legend:f4|comment:HCA mutants R1156M and V1287E showed similar affinity to SV2A and SV2B as wild-type HCA indicating subtle differences between the mode of interaction of SV2A/B and of SV2C with BoNT/A|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 rogid:PnkAiRP4l+Bkf3cK1gDGTPCsT/810116 intact-crc:95B742EBA31797E4|rigid:7P2uuCdUTVkFX9T7CdRZckkemPI false tandem tag:?-?|binding-associated region:423-1251|mutation disrupting interaction:1292-1292 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P0DPI0 uniprotkb:Q02563 intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845 intact:EBI-466194|uniprotkb:Q58DZ8|ensembl:ENSRNOP00000028760 psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym) psi-mi:sv2a_rat(display_long)|uniprotkb:Sv2a(gene name)|psi-mi:Sv2a(display_short)|uniprotkb:Sv2(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0471"(MINT) intact:EBI-9119119|imex:IM-22230-13 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding) rgd:619715|refseq:NP_476558.2|refseq:XP_006233014.1|ensembl:ENSRNOT00000028760(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0014052"(regulation of gamma-aminobutyric acid secretion)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016082"(synaptic vesicle priming)|go:"GO:0019901"(protein kinase binding)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030425"(dendrite)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0045202"(synapse)|go:"GO:0048786"(presynaptic active zone)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259|mint:Q02563|ensembl:ENSRNOG00000021182(gene) - comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure" - figure legend:f4|comment:HCA mutants R1156M and V1287E showed similar affinity to SV2A and SV2B as wild-type HCA indicating subtle differences between the mode of interaction of SV2A/B and of SV2C with BoNT/A|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491 rogid:a7TA6gTNT5eWAIVLxjT1dgUQzn410116 intact-crc:95B742EBA31797E4|rigid:7P2uuCdUTVkFX9T7CdRZckkemPI false tandem tag:?-?|binding-associated region:423-1251|mutation disrupting interaction:1292-1292 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q00496 uniprotkb:Q63564 intact:EBI-9119157|uniprotkb:Q45862 intact:EBI-9254006 psi-mi:bxe_clobo(display_long)|uniprotkb:botE(gene name)|psi-mi:botE(display_short)|uniprotkb:Bontoxilysin-E(gene name synonym) psi-mi:sv2b_rat(display_long)|uniprotkb:Sv2b(gene name)|psi-mi:Sv2b(display_short)|uniprotkb:Sv2bb(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0471"(MINT) intact:EBI-9119190|imex:IM-22230-14 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:WP_003372387.1|reactome:R-HSA-5250992|dip:DIP-46083N|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008289"(lipid binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|rcsb pdb:1T3A|rcsb pdb:1T3C|rcsb pdb:1ZKW|rcsb pdb:1ZKX|rcsb pdb:1ZL5|rcsb pdb:1ZL6|rcsb pdb:1ZN3|rcsb pdb:3FFZ|rcsb pdb:7K7Y|rcsb pdb:7K84 interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR022308|mint:Q63564|rgd:619716|refseq:NP_476555.1|interpro:IPR036259|go:"GO:0001504"(neurotransmitter uptake)|go:"GO:0001669"(acrosomal vesicle)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|go:"GO:0043195"(terminal bouton) - - - figure legend:f4|comment:HCA mutants R1156M and V1287E showed similar affinity to SV2A and SV2B as wild-type HCA indicating subtle differences between the mode of interaction of SV2A/B and of SV2C with BoNT/A|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:zOobvRGIyO5Ji/AoLT/+CNWua001491 rogid:PnkAiRP4l+Bkf3cK1gDGTPCsT/810116 intact-crc:22275B4D1811F069|rigid:imXaCUupO6pQlUHq0CF1KOTXuA8 false binding-associated region:423-1251|tandem tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q00496 uniprotkb:Q02563 intact:EBI-9119157|uniprotkb:Q45862 intact:EBI-466194|uniprotkb:Q58DZ8|ensembl:ENSRNOP00000028760 psi-mi:bxe_clobo(display_long)|uniprotkb:botE(gene name)|psi-mi:botE(display_short)|uniprotkb:Bontoxilysin-E(gene name synonym) psi-mi:sv2a_rat(display_long)|uniprotkb:Sv2a(gene name)|psi-mi:Sv2a(display_short)|uniprotkb:Sv2(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) - (2014) imex:IM-22230|pubmed:24583011 taxid:1491(clobo)|taxid:1491(Clostridium botulinum) taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0471"(MINT) intact:EBI-9119190|imex:IM-22230-14 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:WP_003372387.1|reactome:R-HSA-5250992|dip:DIP-46083N|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008289"(lipid binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|rcsb pdb:1T3A|rcsb pdb:1T3C|rcsb pdb:1ZKW|rcsb pdb:1ZKX|rcsb pdb:1ZL5|rcsb pdb:1ZL6|rcsb pdb:1ZN3|rcsb pdb:3FFZ|rcsb pdb:7K7Y|rcsb pdb:7K84 rgd:619715|refseq:NP_476558.2|refseq:XP_006233014.1|ensembl:ENSRNOT00000028760(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0006874"(cellular calcium ion homeostasis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0014052"(regulation of gamma-aminobutyric acid secretion)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016082"(synaptic vesicle priming)|go:"GO:0019901"(protein kinase binding)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030425"(dendrite)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0045202"(synapse)|go:"GO:0048786"(presynaptic active zone)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0098982"(GABA-ergic synapse)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259|mint:Q02563|ensembl:ENSRNOG00000021182(gene) - - - figure legend:f4|comment:HCA mutants R1156M and V1287E showed similar affinity to SV2A and SV2B as wild-type HCA indicating subtle differences between the mode of interaction of SV2A/B and of SV2C with BoNT/A|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/02/06 2014/10/16 rogid:zOobvRGIyO5Ji/AoLT/+CNWua001491 rogid:a7TA6gTNT5eWAIVLxjT1dgUQzn410116 intact-crc:22275B4D1811F069|rigid:imXaCUupO6pQlUHq0CF1KOTXuA8 false binding-associated region:423-1251|tandem tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot)